Gene Symbol: Arhgef2
Description: Rho/Rac guanine nucleotide exchange factor 2
Alias: rho guanine nucleotide exchange factor 2, GEF-H1, guanine nucleotide exchange factor H1, rho/rac guanine nucleotide exchange factor (GEF) 2
Species: rat
Products:     Arhgef2

Top Publications

  1. Ren Y, Li R, Zheng Y, Busch H. Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases. J Biol Chem. 1998;273:34954-60 pubmed
    ..These results suggest that GEF-H1 may have a direct role in activation of Rac and/or Rho and in bringing the activated GTPase to specific target sites such as microtubules. ..
  2. Krendel M, Zenke F, Bokoch G. Nucleotide exchange factor GEF-H1 mediates cross-talk between microtubules and the actin cytoskeleton. Nat Cell Biol. 2002;4:294-301 pubmed
    ..Furthermore, these effects are inhibited by dominant-negative versions of GEF-H1. Thus, GEF-H1 links changes in microtubule integrity to Rho-dependent regulation of the actin cytoskeleton. ..
  3. Mouawad F, Aoudjit L, Jiang R, Szaszi K, Takano T. Role of guanine nucleotide exchange factor-H1 in complement-mediated RhoA activation in glomerular epithelial cells. J Biol Chem. 2014;289:4206-18 pubmed publisher
    ..In summary, we have identified complement-induced ERK-dependent GEF-H1 activation as the upstream mechanism of RhoA stimulation, and this pathway has a protective role against cell death. ..
  4. Shiba Y, Randazzo P. GEFH1 binds ASAP1 and regulates podosome formation. Biochem Biophys Res Commun. 2011;406:574-9 pubmed publisher
    ..Reduced expression of GEFH1, achieved with siRNA treatment, did not affect matrix degradation by podosomes but increased the rate of podosome assembly. Based on these results, we conclude that GEFH1 is a negative regulator of podosomes. ..
  5. Gauthier Fisher A, Lin D, Greeve M, Kaplan D, Rottapel R, Miller F. Lfc and Tctex-1 regulate the genesis of neurons from cortical precursor cells. Nat Neurosci. 2009;12:735-44 pubmed publisher
    ..We found that Lfc (Arhgef2), a Rho-specific guanine nucleotide exchange factor that interacts with spindle microtubules, and its negative ..
  6. Luo T, Roman P, Liu C, Sun X, Park Y, Hu B. Upregulation of the GEF-H1 pathway after transient cerebral ischemia. Exp Neurol. 2015;263:306-13 pubmed publisher
    ..The initial upregulation of GEF-H1 activity may contribute to excitotoxicity, whereas the late upregulation of GEF-H1 protein may promote neuroplasticity after brain ischemia. ..
  7. Torres Cruz F, Rodríguez Cruz F, Escobar Herrera J, Barragán Andrade N, Basurto Islas G, Ripova D, et al. Expression of Tau Produces Aberrant Plasma Membrane Blebbing in Glial Cells Through RhoA-ROCK-Dependent F-Actin Remodeling. J Alzheimers Dis. 2016;52:463-82 pubmed publisher
    ..In AD and other tauopathies, these Tau-induced abnormalities may occur and contribute to the impairment of glial activity. ..
  8. Kang M, Guo Y, Huganir R. AMPA receptor and GEF-H1/Lfc complex regulates dendritic spine development through RhoA signaling cascade. Proc Natl Acad Sci U S A. 2009;106:3549-54 pubmed publisher
    ..Altogether, these results strongly suggest that GEF-H1 is an important mediator of AMPA-R activity-dependent structural plasticity in neurons. ..
  9. Nie M, Balda M, Matter K. Stress- and Rho-activated ZO-1-associated nucleic acid binding protein binding to p21 mRNA mediates stabilization, translation, and cell survival. Proc Natl Acad Sci U S A. 2012;109:10897-902 pubmed publisher
    ..We thus identify a unique type of stress and Rho signaling activated pathway that drives mRNA stabilization and translation and links the cellular stress response to p21 expression and cell survival...

More Information


  1. Sabirzhanova I, Liu C, Zhao J, Bramlett H, Dietrich W, Hu B. Changes in the GEF-H1 pathways after traumatic brain injury. J Neurotrauma. 2013;30:1449-56 pubmed publisher
  2. Pathak R, DerMardirossian C. GEF-H1: orchestrating the interplay between cytoskeleton and vesicle trafficking. Small Gtpases. 2013;4:174-9 pubmed publisher
    ..These results provide a framework for understanding how RhoA/GEF-H1 regulates the coordination of actin and microtubule cytoskeleton modulation and vesicle trafficking during migration and cell division. ..
  3. Ravindran E, Hu H, Yuzwa S, Hernández Miranda L, Kraemer N, Ninnemann O, et al. Homozygous ARHGEF2 mutation causes intellectual disability and midbrain-hindbrain malformation. PLoS Genet. 2017;13:e1006746 pubmed publisher
    ..We identified, by means of whole exome sequencing, a homozygous frameshift mutation in the ARHGEF2 as a cause of intellectual disability, a midbrain-hindbrain malformation, and mild microcephaly in a ..
  4. Ryan X, Alldritt J, Svenningsson P, Allen P, Wu G, Nairn A, et al. The Rho-specific GEF Lfc interacts with neurabin and spinophilin to regulate dendritic spine morphology. Neuron. 2005;47:85-100 pubmed
    ..Thus, interaction between Lfc and neurabin/spinophilin selectively regulates Rho-dependent organization of F-actin in spines and is a link between the microtubule and F-actin cytoskeletons in dendrites. ..
  5. Fukazawa A, Alonso C, Kurachi K, Gupta S, Lesser C, McCormick B, et al. GEF-H1 mediated control of NOD1 dependent NF-kappaB activation by Shigella effectors. PLoS Pathog. 2008;4:e1000228 pubmed publisher
    ..The guanine nucleotide exchange factor H1 (GEF-H1) mediates RhoA activation required for cell invasion by the enteroinvasive pathogen ..
  6. Zhao Y, Alonso C, Ballester I, Song J, Chang S, Guleng B, et al. Control of NOD2 and Rip2-dependent innate immune activation by GEF-H1. Inflamm Bowel Dis. 2012;18:603-12 pubmed publisher
    ..We utilized gene-specific siRNA mediated knockdown and expression of guanine nucleotide exchange factor H1 (GEF-H1) in wildtype, Rip2-, and Nod2-deficient macrophages, HCT-116 and HEK 293 cells to ..
  7. Arnette C, Frye K, Kaverina I. Microtubule and Actin Interplay Drive Intracellular c-Src Trafficking. PLoS ONE. 2016;11:e0148996 pubmed publisher
    ..Our results indicate that regulation of c-Src trafficking requires both microtubules and actin polymerization, and that GEF-H1 coordinates c-Src trafficking, acting as a molecular switch between these two mechanisms. ..