Gene Symbol: Ap2b1
Description: adaptor-related protein complex 2, beta 1 subunit
Alias: AP-2 complex subunit beta, AP-1 complex subunit beta-1-like, AP105B, adapter-related protein complex 2 beta subunit, adapter-related protein complex 2 subunit beta, adaptor protein complex AP-2 subunit beta, adaptor-related protein complex 2 subunit beta, beta-2-adaptin, beta-adaptin, beta2-adaptin, clathrin assembly protein complex 2 beta large chain, plasma membrane adaptor HA2/AP2 adaptin beta subunit
Species: rat
Products:     Ap2b1

Top Publications

  1. Ponnambalam S, Robinson M, Jackson A, Peiperl L, Parham P. Conservation and diversity in families of coated vesicle adaptins. J Biol Chem. 1990;265:4814-20 pubmed
    The complete sequence of the beta adaptin subunit of the plasma membrane adaptor complex from coated vesicles has been elucidated...
  2. Saheki Y, De Camilli P. Synaptic vesicle endocytosis. Cold Spring Harb Perspect Biol. 2012;4:a005645 pubmed publisher
  3. Hao W, Luo Z, Zheng L, Prasad K, Lafer E. AP180 and AP-2 interact directly in a complex that cooperatively assembles clathrin. J Biol Chem. 1999;274:22785-94 pubmed
    ..Phosphorylation of AP180, by modulating the affinity of AP180 for AP-2, may contribute to the regulation of clathrin assembly in vivo. ..
  4. Bai M, Gad H, Turacchio G, Cocucci E, Yang J, Li J, et al. ARFGAP1 promotes AP-2-dependent endocytosis. Nat Cell Biol. 2011;13:559-67 pubmed publisher
    ..The role of ARFGAP1 in AP-2-regulated endocytosis has mechanistic parallels with its roles in COPI transport, as both its GAP activity and coat function contribute to promoting AP-2 transport. ..
  5. Rudinskiy N, Grishchuk Y, Vaslin A, Puyal J, Delacourte A, Hirling H, et al. Calpain hydrolysis of alpha- and beta2-adaptins decreases clathrin-dependent endocytosis and may promote neurodegeneration. J Biol Chem. 2009;284:12447-58 pubmed publisher
  6. Mishra S, Keyel P, Hawryluk M, Agostinelli N, Watkins S, Traub L. Disabled-2 exhibits the properties of a cargo-selective endocytic clathrin adaptor. EMBO J. 2002;21:4915-26 pubmed
  7. Tokumitsu H, Hatano N, Yokokura S, Sueyoshi Y, Nozaki N, Kobayashi R. Phosphorylation of Numb regulates its interaction with the clathrin-associated adaptor AP-2. FEBS Lett. 2006;580:5797-801 pubmed
    ..These findings suggest a novel mechanism for the regulation of Numb-mediated endocytosis, namely through direct phosphorylation. ..
  8. Page L, Robinson M. Targeting signals and subunit interactions in coated vesicle adaptor complexes. J Cell Biol. 1995;131:619-30 pubmed
    ..These results indicate that the adaptor subunits act in concert to target the complex to the appropriate membrane. ..
  9. Slepnev V, Ochoa G, Butler M, Grabs D, De Camilli P. Role of phosphorylation in regulation of the assembly of endocytic coat complexes. Science. 1998;281:821-4 pubmed
    ..Thus, phosphorylation regulates the association and dissociation cycle of the clathrin-based endocytic machinery, and calcium-dependent dephosphorylation of endocytic proteins could prepare nerve terminals for a burst of endocytosis. ..

More Information


  1. Mishra S, Keyel P, Edeling M, Dupin A, Owen D, Traub L. Functional dissection of an AP-2 beta2 appendage-binding sequence within the autosomal recessive hypercholesterolemia protein. J Biol Chem. 2005;280:19270-80 pubmed
    ..We suggested that biased beta subunit engagement by ARH and the only other beta2 appendage selective adaptor, beta-arrestin, promotes efficient incorporation of this mechanistically distinct subset of CLASPs into clathrin-coated buds. ..
  2. Matsuda S, Kakegawa W, Budisantoso T, Nomura T, Kohda K, Yuzaki M. Stargazin regulates AMPA receptor trafficking through adaptor protein complexes during long-term depression. Nat Commun. 2013;4:2759 pubmed publisher
  3. Hood F, Royle S. Functional equivalence of the clathrin heavy chains CHC17 and CHC22 in endocytosis and mitosis. J Cell Sci. 2009;122:2185-90 pubmed publisher
    ..We find that CHC17 and CHC22 are functionally equivalent. We also describe how previous work on CHC22 has involved a splice variant that is not usually expressed in cells. ..
  4. Fukamachi K, Matsuoka Y, Ohno H, Hamaguchi T, Tsuda H. Neuronal leucine-rich repeat protein-3 amplifies MAPK activation by epidermal growth factor through a carboxyl-terminal region containing endocytosis motifs. J Biol Chem. 2002;277:43549-52 pubmed
    ..We propose that NLRR-3 potentiates Ras-MAPK signaling by facilitating internalization of EGF in clathrin-coated vesicles. ..
  5. Collins B, McCoy A, Kent H, Evans P, Owen D. Molecular architecture and functional model of the endocytic AP2 complex. Cell. 2002;109:523-35 pubmed
    ..A model for AP2 recruitment and activation is proposed. ..
  6. Drake M, Traub L. Interaction of two structurally distinct sequence types with the clathrin terminal domain beta-propeller. J Biol Chem. 2001;276:28700-9 pubmed
    ..Together, the data provide evidence for two interaction surfaces upon certain endocytic accessory proteins that could cooperate with other coat components to enhance clathrin bud formation at the cell surface. ..
  7. Gallusser A, Kirchhausen T. The beta 1 and beta 2 subunits of the AP complexes are the clathrin coat assembly components. EMBO J. 1993;12:5237-44 pubmed
    ..It is proposed that the principal function of the beta subunits is to initiate coat formation, while the remaining subunits of the AP complexes have other roles in coated pit and coated vesicle function...
  8. Kirchhausen T, Nathanson K, Matsui W, Vaisberg A, Chow E, Burne C, et al. Structural and functional division into two domains of the large (100- to 115-kDa) chains of the clathrin-associated protein complex AP-2. Proc Natl Acad Sci U S A. 1989;86:2612-6 pubmed
    ..Their amino-terminal domains are relatively invariant whereas their carboxyl-terminal domains are variable in both sequence and length. We propose that the variable domains select receptors for inclusion in coated vesicles. ..
  9. Morgan J, Prasad K, Hao W, Augustine G, Lafer E. A conserved clathrin assembly motif essential for synaptic vesicle endocytosis. J Neurosci. 2000;20:8667-76 pubmed
    ..These results reveal the structural basis for clathrin assembly and provide novel insights into the molecular mechanism of clathrin-mediated synaptic vesicle endocytosis. ..