Gene Symbol: Ap2a1
Description: adaptor-related protein complex 2, alpha 1 subunit
Alias: AP-2 complex subunit alpha-1, adaptor protein complex AP-2, alpha 1 subunit, alpha-adaptin A
Species: rat
Products:     Ap2a1

Top Publications

  1. Drake M, Traub L. Interaction of two structurally distinct sequence types with the clathrin terminal domain beta-propeller. J Biol Chem. 2001;276:28700-9 pubmed
    ..Together, the data provide evidence for two interaction surfaces upon certain endocytic accessory proteins that could cooperate with other coat components to enhance clathrin bud formation at the cell surface. ..
  2. Legendre Guillemin V, Metzler M, Charbonneau M, Gan L, Chopra V, Philie J, et al. HIP1 and HIP12 display differential binding to F-actin, AP2, and clathrin. Identification of a novel interaction with clathrin light chain. J Biol Chem. 2002;277:19897-904 pubmed
    ..Interestingly, in both HIP1 and HIP12, this domain binds directly to the clathrin light chain. Our data suggest that HIP1 and HIP12 play related yet distinct functional roles in clathrin-mediated endocytosis. ..
  3. Moulakakis C, Steinhäuser C, Biedziak D, Freundt K, Reiling N, Stamme C. Surfactant Protein A Enhances Constitutive Immune Functions of Clathrin Heavy Chain and Clathrin Adaptor Protein 2. Am J Respir Cell Mol Biol. 2016;55:92-104 pubmed publisher
  4. Onishi K, Shafer B, Lo C, Tissir F, Goffinet A, Zou Y. Antagonistic functions of Dishevelleds regulate Frizzled3 endocytosis via filopodia tips in Wnt-mediated growth cone guidance. J Neurosci. 2013;33:19071-85 pubmed publisher
  5. Wasiak S, Legendre Guillemin V, Puertollano R, Blondeau F, Girard M, de Heuvel E, et al. Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics. J Cell Biol. 2002;158:855-62 pubmed
    ..These data suggest a role for enthoprotin in clathrin-mediated budding on internal membranes. Our study reveals the utility of proteomics in the identification of novel vesicle trafficking proteins...
  6. Duncker S, Franz C, Kuhn S, Schulte U, Campanelli D, Brandt N, et al. Otoferlin couples to clathrin-mediated endocytosis in mature cochlear inner hair cells. J Neurosci. 2013;33:9508-19 pubmed publisher
    ..We suggest that an otoferlin-AP-2 interaction drives Ca(2+)- and stimulus-dependent compensating CME in mature IHCs. ..
  7. Rotem Yehudar R, Galperin E, Horowitz M. Association of insulin-like growth factor 1 receptor with EHD1 and SNAP29. J Biol Chem. 2001;276:33054-60 pubmed
  8. Bai M, Gad H, Turacchio G, Cocucci E, Yang J, Li J, et al. ARFGAP1 promotes AP-2-dependent endocytosis. Nat Cell Biol. 2011;13:559-67 pubmed publisher
    ..The role of ARFGAP1 in AP-2-regulated endocytosis has mechanistic parallels with its roles in COPI transport, as both its GAP activity and coat function contribute to promoting AP-2 transport. ..
  9. Wigge P, Vallis Y, McMahon H. Inhibition of receptor-mediated endocytosis by the amphiphysin SH3 domain. Curr Biol. 1997;7:554-60 pubmed
    ..We propose that amphiphysin is not only required for synaptic-vesicle endocytosis, but might also be a key player in dynamin recruitment in all cells undergoing receptor-mediated endocytosis. ..

More Information


  1. Mishra S, Keyel P, Hawryluk M, Agostinelli N, Watkins S, Traub L. Disabled-2 exhibits the properties of a cargo-selective endocytic clathrin adaptor. EMBO J. 2002;21:4915-26 pubmed
  2. Hayashi H, Inamura K, Aida K, Naoi S, Horikawa R, Nagasaka H, et al. AP2 adaptor complex mediates bile salt export pump internalization and modulates its hepatocanalicular expression and transport function. Hepatology. 2012;55:1889-900 pubmed publisher
  3. Slepnev V, Ochoa G, Butler M, Grabs D, De Camilli P. Role of phosphorylation in regulation of the assembly of endocytic coat complexes. Science. 1998;281:821-4 pubmed
    ..Thus, phosphorylation regulates the association and dissociation cycle of the clathrin-based endocytic machinery, and calcium-dependent dephosphorylation of endocytic proteins could prepare nerve terminals for a burst of endocytosis. ..
  4. Zhao Z, Sagare A, Ma Q, Halliday M, Kong P, Kisler K, et al. Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance. Nat Neurosci. 2015;18:978-87 pubmed publisher
    ..Thus, PICALM regulates Aβ BBB transcytosis and clearance, which has implications for Aβ brain homeostasis and clearance therapy. ..
  5. Yoshida H, Nagaoka A, Kusaka Kikushima A, Tobiishi M, Kawabata K, Sayo T, et al. KIAA1199, a deafness gene of unknown function, is a new hyaluronan binding protein involved in hyaluronan depolymerization. Proc Natl Acad Sci U S A. 2013;110:5612-7 pubmed publisher
    ..These data suggest that KIAA1199 is a unique hyaladherin with a key role in HA catabolism in the dermis of the skin and arthritic synovium. ..
  6. Di Paolo G, Sankaranarayanan S, Wenk M, Daniell L, Perucco E, Caldarone B, et al. Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice. Neuron. 2002;33:789-804 pubmed
    ..These defects correlate with increased mortality due to rare irreversible seizures and with major learning deficits, suggesting a critical role of amphiphysin for higher brain functions. ..
  7. Kamiguchi H, Yoshihara F. The role of endocytic l1 trafficking in polarized adhesion and migration of nerve growth cones. J Neurosci. 2001;21:9194-203 pubmed
    ..These results indicate that the growth cone actively translocates CAMs to create spatial asymmetry in adhesive interactions with its environment and that this spatial asymmetry is important for growth cone migration. ..
  8. Saheki Y, De Camilli P. Synaptic vesicle endocytosis. Cold Spring Harb Perspect Biol. 2012;4:a005645 pubmed publisher
  9. Nashida T, Yoshie S, Imai A, Shimomura H. Co-localization of rab4 with endocytosis-related proteins in the rat parotid glands. Arch Histol Cytol. 2003;66:45-52 pubmed
    ..Rab5A immunoreactivity was distributed all around the cytoplasm. These findings suggested that Rab4 participates in endocytosis at the apical membrane of parotid glands. ..
  10. Yoshida H, Nagaoka A, Nakamura S, Sugiyama Y, Okada Y, Inoue S. Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization. FEBS Open Bio. 2013;3:352-6 pubmed publisher
    ..We conclude that like hKIAA1199, mKiaa1199 is a hyaladherin, leading to HA depolymerization. ..
  11. Chen H, Slepnev V, Di Fiore P, De Camilli P. The interaction of epsin and Eps15 with the clathrin adaptor AP-2 is inhibited by mitotic phosphorylation and enhanced by stimulation-dependent dephosphorylation in nerve terminals. J Biol Chem. 1999;274:3257-60 pubmed