Gene Symbol: Akap5
Description: A-kinase anchoring protein 5
Alias: AKAP150, Akap79, P150, A-kinase anchor protein 5, A kinase (PRKA) anchor protein 5, A-kinase anchor protein 150 kDa, AKAP 150, AKAP-5, RII-B-binding protein, cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
Species: rat
Products:     Akap5

Top Publications

  1. Zhang X, Li L, McNaughton P. Proinflammatory mediators modulate the heat-activated ion channel TRPV1 via the scaffolding protein AKAP79/150. Neuron. 2008;59:450-61 pubmed publisher
    ..calcineurin depends on the formation of a signaling complex between these enzymes, the scaffolding protein AKAP79/150 and TRPV1. We identify a critical region in the TRPV1 C-terminal which mediates binding of AKAP79/150...
  2. Jeske N, Patwardhan A, Ruparel N, Akopian A, Shapiro M, Henry M. A-kinase anchoring protein 150 controls protein kinase C-mediated phosphorylation and sensitization of TRPV1. Pain. 2009;146:301-7 pubmed publisher
    ..Recent studies have reported that the A-kinase Anchoring Protein 150 (AKAP150) mediates PKA phosphorylation of TRPV1 in several nociceptive models...
  3. Gorski J, Gomez L, Scott J, Dell Acqua M. Association of an A-kinase-anchoring protein signaling scaffold with cadherin adhesion molecules in neurons and epithelial cells. Mol Biol Cell. 2005;16:3574-90 pubmed
    ..b>AKAP79/150 postsynaptic targeting requires three N-terminal basic domains that bind F-actin and acidic phospholipids...
  4. Bhattacharyya S, Biou V, Xu W, Schlüter O, Malenka R. A critical role for PSD-95/AKAP interactions in endocytosis of synaptic AMPA receptors. Nat Neurosci. 2009;12:172-81 pubmed publisher
    ..endocytosis of synaptic AMPARs in rat neuron cultures because of its binding to A kinase-anchoring protein 150 (AKAP150), a scaffold for specific protein kinases and phosphatases...
  5. Jeske N, Diogenes A, Ruparel N, Fehrenbacher J, Henry M, Akopian A, et al. A-kinase anchoring protein mediates TRPV1 thermal hyperalgesia through PKA phosphorylation of TRPV1. Pain. 2008;138:604-16 pubmed publisher
    ..In this study, we investigated the functional involvement of AKAP150 in mediating sensitization of TRPV1, and found that AKAP150 is co-expressed in trigeminal ganglia (TG) neurons ..
  6. Gomez L, Alam S, Smith K, Horne E, Dell Acqua M. Regulation of A-kinase anchoring protein 79/150-cAMP-dependent protein kinase postsynaptic targeting by NMDA receptor activation of calcineurin and remodeling of dendritic actin. J Neurosci. 2002;22:7027-44 pubmed
    ..synapses, the cAMP-dependent protein kinase (PKA), protein kinase C (PKC), and calcineurin (CaN) anchoring protein AKAP79/150 is recruited to NMDA and AMPA glutamate receptors by postsynaptic density (PSD)-95 family membrane-associated ..
  7. Smith K, Gibson E, Dell Acqua M. cAMP-dependent protein kinase postsynaptic localization regulated by NMDA receptor activation through translocation of an A-kinase anchoring protein scaffold protein. J Neurosci. 2006;26:2391-402 pubmed
    ..Here, we report that brief NMDA receptor activation leads to persistent redistribution of AKAP79/150 and PKA-RII, but not PP2B/CaN, from postsynaptic membranes to the cytoplasm in hippocampal slices...
  8. Robertson H, Gibson E, Benke T, Dell Acqua M. Regulation of postsynaptic structure and function by an A-kinase anchoring protein-membrane-associated guanylate kinase scaffolding complex. J Neurosci. 2009;29:7929-43 pubmed publisher
    ..In cultured hippocampal neurons, we observed that rat AKAP150 expression was low early in development but then increased coincident with spine formation and maturation...
  9. Bregman D, Bhattacharyya N, Rubin C. High affinity binding protein for the regulatory subunit of cAMP-dependent protein kinase II-B. Cloning, characterization, and expression of cDNAs for rat brain P150. J Biol Chem. 1989;264:4648-56 pubmed
    ..of a prototypic, nonabundant, RII-B-binding protein, we have cloned and characterized cDNAs for rat brain P150, a homolog of bovine brain P75...

More Information


  1. Oliveria S, Dell Acqua M, Sather W. AKAP79/150 anchoring of calcineurin controls neuronal L-type Ca2+ channel activity and nuclear signaling. Neuron. 2007;55:261-75 pubmed
    ..Here, we show that CaV1.2 interacts directly with AKAP79/150, which binds both PKA and the Ca2+/calmodulin-activated phosphatase calcineurin (CaN)...
  2. Hoshi N, Zhang J, Omaki M, Takeuchi T, Yokoyama S, Wanaverbecq N, et al. AKAP150 signaling complex promotes suppression of the M-current by muscarinic agonists. Nat Neurosci. 2003;6:564-71 pubmed
    ..We show here that rat KCNQ2 can bind directly to the multivalent A-kinase-anchoring protein AKAP150. Peptides that block AKAP150 binding to the KCNQ2 channel complex antagonize the muscarinic inhibition of the ..
  3. Genin A, French P, Doyere V, Davis S, Errington M, Maroun M, et al. LTP but not seizure is associated with up-regulation of AKAP-150. Eur J Neurosci. 2003;17:331-40 pubmed
  4. Liu G, Shi J, Yang L, Cao L, Park S, Cui J, et al. Assembly of a Ca2+-dependent BK channel signaling complex by binding to beta2 adrenergic receptor. EMBO J. 2004;23:2196-205 pubmed
    ..beta2-adrenergic receptor (beta2AR) with the pore forming alpha subunit of BKCa and an A-kinase-anchoring protein (AKAP79/150) for beta2 agonist regulation...
  5. Hall D, Davare M, Shi M, Allen M, Weisenhaus M, McKnight G, et al. Critical role of cAMP-dependent protein kinase anchoring to the L-type calcium channel Cav1.2 via A-kinase anchor protein 150 in neurons. Biochemistry. 2007;46:1635-46 pubmed
    ..2 subunit. Here we demonstrate that the A-kinase anchor protein 150 (AKAP150) is critical for PKA-mediated regulation of Cav1.2 in the brain...
  6. Colledge M, Dean R, Scott G, Langeberg L, Huganir R, Scott J. Targeting of PKA to glutamate receptors through a MAGUK-AKAP complex. Neuron. 2000;27:107-19 pubmed
    ..a macromolecular signaling complex through direct interaction between the MAGUK proteins, PSD-95 and SAP97, and AKAP79/150. The SH3 and GK regions of the MAGUKs mediate binding to the AKAP...
  7. Chaudhury S, Bal M, Belugin S, Shapiro M, Jeske N. AKAP150-mediated TRPV1 sensitization is disrupted by calcium/calmodulin. Mol Pain. 2011;7:34 pubmed publisher
    ..A-Kinase Anchoring Protein 79/150 (AKAP150) mediates phosphorylation of TRPV1 by Protein Kinases A and C, modulating channel activity...
  8. De Windt L, Lim H, Bueno O, Liang Q, Delling U, Braz J, et al. Targeted inhibition of calcineurin attenuates cardiac hypertrophy in vivo. Proc Natl Acad Sci U S A. 2001;98:3322-7 pubmed
    ..These results provide genetic evidence implicating calcineurin as an important mediator of the cardiac hypertrophic response in vivo. ..
  9. Wang D, Govindaiah G, Liu R, De Arcangelis V, Cox C, Xiang Y. Binding of amyloid beta peptide to beta2 adrenergic receptor induces PKA-dependent AMPA receptor hyperactivity. FASEB J. 2010;24:3511-21 pubmed publisher
    ..beta(2)AR and GluR1 also form a complex comprising postsynaptic density protein 95 (PSD95), PKA and its anchor AKAP150, and protein phosphotase 2A (PP2A)...
  10. Ku C, Sanborn B. Progesterone prevents the pregnancy-related decline in protein kinase A association with rat myometrial plasma membrane and A-kinase anchoring protein. Biol Reprod. 2002;67:605-9 pubmed
    ..protein kinase (PKA) in the plasma membrane compartment and its association with an A-kinase anchoring protein (AKAP150) is implicated in mediating cAMP regulatory events in the rat myometrium...
  11. Brandao K, Dell Acqua M, Levinson S. A-kinase anchoring protein 150 expression in a specific subset of TRPV1- and CaV 1.2-positive nociceptive rat dorsal root ganglion neurons. J Comp Neurol. 2012;520:81-99 pubmed publisher
    ..However, the expression pattern of AKAP150 in peripheral sensory neurons is unknown...
  12. Nikandrova Y, Jiao Y, Baucum A, Tavalin S, Colbran R. Ca2+/calmodulin-dependent protein kinase II binds to and phosphorylates a specific SAP97 splice variant to disrupt association with AKAP79/150 and modulate alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type glutamate receptor (AMPAR) activ. J Biol Chem. 2010;285:923-34 pubmed publisher
    ..complexes coordinated by synapse-associated protein 97 (SAP97) that contain A-kinase anchoring protein 79/150 (AKAP79/150), protein kinase A, and protein phosphatase 2B...
  13. Lee J, Chung M, Ro J. Activation of NMDA receptors leads to phosphorylation of TRPV1 S800 by protein kinase C and A-Kinase anchoring protein 150 in rat trigeminal ganglia. Biochem Biophys Res Commun. 2012;424:358-63 pubmed publisher
    A-Kinase anchoring protein 150 (AKAP150) is required for the phosphorylation of transient receptor potential cation channel subfamily V member 1 (TRPV1) by PKA or PKC in sensory neurons and, hence, affects TRPV1-dependent hyperalgesia ..
  14. Tasken K, Aandahl E. Localized effects of cAMP mediated by distinct routes of protein kinase A. Physiol Rev. 2004;84:137-67 pubmed
  15. Gardner L, Tavalin S, Goehring A, Scott J, Bahouth S. AKAP79-mediated targeting of the cyclic AMP-dependent protein kinase to the beta1-adrenergic receptor promotes recycling and functional resensitization of the receptor. J Biol Chem. 2006;281:33537-53 pubmed
    ..several endogenous AKAPs were identified in HEK-293 cells, small interfering RNA-mediated down-regulation of AKAP79 prevented the recycling of the beta(1)-AR in this cell line...
  16. Hoshi N, Scott J. Contextual utilization of enzymes in discrete AKAP79/150 signalling complexes. Eur J Cell Biol. 2006;85:621-2 pubmed
    ..We have developed a new strategy that combines RNA interference of the endogenous protein and replacement with AKAP79/150 forms unable to anchor selected binding partners...
  17. Swayze R, Lise M, Levinson J, Phillips A, El Husseini A. Modulation of dopamine mediated phosphorylation of AMPA receptors by PSD-95 and AKAP79/150. Neuropharmacology. 2004;47:764-78 pubmed
    ..These effects were disrupted by expression of mutant forms of the A-kinase anchoring protein (AKAP79/150) and the postsynaptic density protein, PSD-95, that fail to target synaptic sites...
  18. Chung M, Lee J, Joseph J, Saloman J, Ro J. Peripheral group I metabotropic glutamate receptor activation leads to muscle mechanical hyperalgesia through TRPV1 phosphorylation in the rat. J Pain. 2015;16:67-76 pubmed publisher
    ..article demonstrates that activation of mGlu1/5 leads to phosphorylation of a specific TRPV1 residue via PKC and AKAP150 in trigeminal sensory neurons and that functional interactions between glutamate receptors and TRPV1 mediate ..
  19. Seyler C, Scherer D, Köpple C, Kulzer M, Korkmaz S, Xynogalos P, et al. Role of plasma membrane-associated AKAPs for the regulation of cardiac IK1 current by protein kinase A. Naunyn Schmiedebergs Arch Pharmacol. 2017;390:493-503 pubmed publisher
    ..We observed functional and spatial coupling of the plasma membrane-associated AKAP15 and AKAP79 to Kir2.1 and Kir2.2 channel subunits, but not to Kir2.3 channels...
  20. Horne E, Dell Acqua M. Phospholipase C is required for changes in postsynaptic structure and function associated with NMDA receptor-dependent long-term depression. J Neurosci. 2007;27:3523-34 pubmed
    ..The scaffold proteins A-kinase-anchoring protein 79/150 (AKAP79/150) and postsynaptic density 95 (PSD95) form a complex that controls the opposing actions of the cAMP-dependent ..
  21. Willoughby D, Masada N, Wachten S, Pagano M, Halls M, Everett K, et al. AKAP79/150 interacts with AC8 and regulates Ca2+-dependent cAMP synthesis in pancreatic and neuronal systems. J Biol Chem. 2010;285:20328-42 pubmed publisher
    ..and high resolution live cell imaging techniques, we reveal an association of the Ca(2+)-stimulable AC8 with AKAP79/150 that limits the sensitivity of AC8 to intracellular Ca(2+) events...
  22. Jurado S, Biou V, Malenka R. A calcineurin/AKAP complex is required for NMDA receptor-dependent long-term depression. Nat Neurosci. 2010;13:1053-5 pubmed publisher
    b>AKAP79/150 is a protein scaffold that is thought to position specific kinases (protein kinase A and C) and phosphatases (calcineurin) in appropriate synaptic domains so that their activities can regulate excitatory synaptic strength...
  23. Lee Y, Koo Y, Cho C, Seo M, Kang U, Kim Y, et al. Repeated electroconvulsive shock treatment increases the expression of A kinase anchoring proteins in the rat hippocampus. Neurosci Lett. 2004;355:213-6 pubmed
    ..Repeated ECS treatment for 10 days resulted in increases in the levels of the protein and mRNA of AKAP150, yotiao, and ezrin in the rat hippocampus...
  24. Dodge K, Carr D, Yue C, Sanborn B. A role for AKAP (A kinase anchoring protein) scaffolding in the loss of a cyclic adenosine 3',5'-monophosphate inhibitory response in late pregnant rat myometrium. Mol Endocrinol. 1999;13:1977-87 pubmed
    ..More PKA was associated with AKAP150 on day 19 than on day 21, while the reverse was true for PP2B...
  25. Ruppelt A, Mosenden R, Grönholm M, Aandahl E, Tobin D, Carlson C, et al. Inhibition of T cell activation by cyclic adenosine 5'-monophosphate requires lipid raft targeting of protein kinase A type I by the A-kinase anchoring protein ezrin. J Immunol. 2007;179:5159-68 pubmed
    ..Finally, small interfering RNA-mediated knockdown of Ezrin abrogates cAMP regulation of IL-2. We propose that Ezrin is essential in the assembly of the cAMP-mediated regulatory pathway that modulates T cell immune responses. ..
  26. Lilly S, Alvarez F, Tietz E. Synaptic and subcellular localization of A-kinase anchoring protein 150 in rat hippocampal CA1 pyramidal cells: Co-localization with excitatory synaptic markers. Neuroscience. 2005;134:155-63 pubmed
    ..One of these is the A-kinase anchoring protein (AKAP150), which confers spatial specificity to protein kinase A and protein phosphatase 2B in the rat brain...
  27. Nauert J, Rigas J, Lester L. Identification of an IQGAP1/AKAP79 complex in beta-cells. J Cell Biochem. 2003;90:97-108 pubmed
    ..of IQGAP1 with PKA was shown to occur through a direct interaction between A kinase anchoring protein 79 (AKAP79) and the carboxyl-terminal domain of IQGAP1...
  28. Zeng C, Wang J, Li N, Shen M, Wang D, Yu Q, et al. AKAP150 mobilizes cPKC-dependent cardiac glucotoxicity. Am J Physiol Endocrinol Metab. 2014;307:E384-97 pubmed publisher
    Activation of conventional PKCs (cPKC) is a key signaling that directs the cardiac toxicity of hyperglycemia. AKAP150, a scaffold protein of the A-kinase anchoring proteins (AKAPs) family, is less defined regarding its capability to ..
  29. Dacher M, Gouty S, Dash S, Cox B, Nugent F. A-kinase anchoring protein-calcineurin signaling in long-term depression of GABAergic synapses. J Neurosci. 2013;33:2650-60 pubmed publisher
    ..local rise in postsynaptic Ca(2+) and CaN activation accompanied by PKA inhibition, which requires AKAP150 as a bridging signaling molecule...
  30. Li H, Pink M, Murphy J, Stein A, Dell Acqua M, Hogan P. Balanced interactions of calcineurin with AKAP79 regulate Ca2+-calcineurin-NFAT signaling. Nat Struct Mol Biol. 2012;19:337-45 pubmed publisher
    In hippocampal neurons, the scaffold protein AKAP79 recruits the phosphatase calcineurin to L-type Ca(2+) channels and couples Ca(2+) influx to activation of calcineurin and of its substrate, the transcription factor NFAT...
  31. Rhim J, Jang I, Yeo E, Song K, Park S. Role of protein kinase C-dependent A-kinase anchoring proteins in lysophosphatidic acid-induced cAMP signaling in human diploid fibroblasts. Aging Cell. 2006;5:451-61 pubmed
    ..We found that levels of protein kinase C (PKC)-dependent AKAPs, such as Gravin and AKAP79, were elevated in senescent cells...
  32. Keith D, Sanderson J, Gibson E, Woolfrey K, Robertson H, Olszewski K, et al. Palmitoylation of A-kinase anchoring protein 79/150 regulates dendritic endosomal targeting and synaptic plasticity mechanisms. J Neurosci. 2012;32:7119-36 pubmed publisher
    ..The A-kinase anchoring protein 79/150 (AKAP79/150) signaling scaffold regulates AMPAR phosphorylation, channel activity, and endosomal trafficking associated ..
  33. Chai S, Li M, Lan J, Xiong Z, Saugstad J, Simon R. A kinase-anchoring protein 150 and calcineurin are involved in regulation of acid-sensing ion channels ASIC1a and ASIC2a. J Biol Chem. 2007;282:22668-77 pubmed
    ..showed expression of ASIC1a and ASIC2a in punctate and clustering patterns in dendrites that co-localized with AKAP150. Inhibition of protein kinase A binding to AKAPs by Ht-31 peptide reduces ASIC currents in cortical neurons and ..
  34. Diering G, Gustina A, Huganir R. PKA-GluA1 coupling via AKAP5 controls AMPA receptor phosphorylation and cell-surface targeting during bidirectional homeostatic plasticity. Neuron. 2014;84:790-805 pubmed publisher
    ..Increased phosphorylation of S845 drove scaling up, while a knockin mutation of S845, or knockdown of the scaffold AKAP5, blocked scaling up...
  35. Jicha G, Weaver C, Lane E, Vianna C, Kress Y, Rockwood J, et al. cAMP-dependent protein kinase phosphorylations on tau in Alzheimer's disease. J Neurosci. 1999;19:7486-94 pubmed
    ..of PKA (Cbeta), the beta II regulatory subunit of PKA (RIIbeta), and the 79 kDa A-kinase-anchoring-protein (AKAP79), are tightly associated with the neurofibrillary pathology, positioning cAMP-dependent protein kinase to ..
  36. Nomura S, Kawanami H, Ueda H, Kizaki T, Ohno H, Izawa T. Possible mechanisms by which adipocyte lipolysis is enhanced in exercise-trained rats. Biochem Biophys Res Commun. 2002;295:236-42 pubmed
    ..The expression of PKA-anchoring protein 150 (AKAP150) in P(40) fraction was greater in exercise-trained (TR) than in control (C) rats...
  37. Kurihara K, Nakanishi N. Regulation of Na,K-ATPase by cAMP-dependent protein kinase anchored on membrane via A-kinase anchoring protein subtype, AKAP-150, in rat parotid gland. Ann N Y Acad Sci. 2003;986:636-8 pubmed
  38. Wu C, DiJulio D, Jacobson K, McKnight G, Watson E. The contribution of AKAP5 in amylase secretion from mouse parotid acini. Am J Physiol Cell Physiol. 2010;298:C1151-8 pubmed publisher
    ..In the present study, AKAP5 (formerly AKAP150) and AKAP6 were identified in mouse parotid acini by type II PKA regulatory subunit (RII) overlay assay and ..
  39. Fraser I, Cong M, Kim J, Rollins E, Daaka Y, Lefkowitz R, et al. Assembly of an A kinase-anchoring protein-beta(2)-adrenergic receptor complex facilitates receptor phosphorylation and signaling. Curr Biol. 2000;10:409-12 pubmed
    ..Here we show that the A-kinase-anchoring protein, AKAP79/150, co-precipitates with the beta(2)-AR in cell and tissue extracts, nucleating a signaling complex that includes ..
  40. Lin L, Sun W, Kung F, Dell Acqua M, Hoffman D. AKAP79/150 impacts intrinsic excitability of hippocampal neurons through phospho-regulation of A-type K+ channel trafficking. J Neurosci. 2011;31:1323-32 pubmed publisher
    ..As part of a previous study, we showed that AKAP79/150 interacts with Kv4.2 complexes and that the two proteins colocalize in hippocampal neurons...
  41. Li X, Nooh M, Bahouth S. Role of AKAP79/150 protein in ?1-adrenergic receptor trafficking and signaling in mammalian cells. J Biol Chem. 2013;288:33797-812 pubmed publisher
    ..We examined the role of AKAP79/150 (AKAP5) in trafficking and signaling of the ?1-adrenergic receptor (?1-AR)...
  42. Kurihara K, Nakanishi N, Amano O, Yamamoto M, Iseki S. Specific expression of an A-kinase anchoring protein subtype, AKAP-150, and specific regulatory mechanism for Na(+),K(+)-ATPase via protein kinase A in the parotid gland among the three major salivary glands of the rat. Biochem Pharmacol. 2003;66:239-50 pubmed
  43. Brandon N, Jovanovic J, Colledge M, Kittler J, Brandon J, Scott J, et al. A-kinase anchoring protein 79/150 facilitates the phosphorylation of GABA(A) receptors by cAMP-dependent protein kinase via selective interaction with receptor beta subunits. Mol Cell Neurosci. 2003;22:87-97 pubmed
    ..Here we establish that the A-kinase anchoring protein 150 (AKAP150), directly binds to the receptor beta1 and beta3, but not to alpha1, alpha2, alpha3, alpha6, beta2, gamma2, or ..
  44. Carnegie G, Means C, Scott J. A-kinase anchoring proteins: from protein complexes to physiology and disease. IUBMB Life. 2009;61:394-406 pubmed publisher
    ..These anchoring proteins form multi-protein complexes that integrate cAMP signaling with other pathways and signaling events. In this review, we focus on recent advances in the elucidation of AKAP function. ..