Gene Symbol: Akap12
Description: A-kinase anchoring protein 12
Alias: AKAP12A, AKAP12B, AKAP12G, A-kinase anchor protein 12, A kinase (PRKA) anchor protein (gravin) 12, A kinase (PRKA) anchor protein 12, AKAP-12, SSeCKS, Gravin, AKAP250
Species: rat
Products:     Akap12

Top Publications

  1. Lin X, Nelson P, Frankfort B, Tombler E, Johnson R, Gelman I. Isolation and characterization of a novel mitogenic regulatory gene, 322, which is transcriptionally suppressed in cells transformed by src and ras. Mol Cell Biol. 1995;15:2754-62 pubmed
    ..Thus, 322 represent a novel src- and ras-regulated gene which encodes a potential regulator of mitogenesis and/or tumor suppressor. ..
  2. Shao B, Li C, Yang H, Shen A, Wu X, Yuan Q, et al. The relationship between Src-suppressed C kinase substrate and ?-1,4 galactosyltransferase-I in the process of lipopolysaccharide-induced TNF-? secretion in rat primary astrocytes. Cell Mol Neurobiol. 2011;31:1047-56 pubmed publisher
    ..Inversely, ?-1,4-GalT-I had no significant effect on SSeCKS expression in the same way. In summary, our data indicated that SSeCKS could regulate LPS-induced TNF-? secretion through ?-1,4-GalT-I in rat primary astrocytes. ..
  3. Zan L, Wu H, Jiang J, Zhao S, Song Y, Teng G, et al. Temporal profile of Src, SSeCKS, and angiogenic factors after focal cerebral ischemia: correlations with angiogenesis and cerebral edema. Neurochem Int. 2011;58:872-9 pubmed publisher
    ..Dynamic temporal changes in Src and SSeCKS expression may modulate angiogenesis and cerebral edema formation after focal cerebral ischemia. ..
  4. Tasken K, Aandahl E. Localized effects of cAMP mediated by distinct routes of protein kinase A. Physiol Rev. 2004;84:137-67 pubmed
  5. Cheng C, Liu H, Ge H, Qian J, Qin J, Sun L, et al. Essential role of Src suppressed C kinase substrates in endothelial cell adhesion and spreading. Biochem Biophys Res Commun. 2007;358:342-8 pubmed
    ..These results demonstrated SSeCKS modulate endothelial cells adhesion and spreading by reorganization of the actin cytoskeleton. ..
  6. Chen L, Qin J, Cheng C, Liu H, Niu S, Qian J, et al. Developmental regulation of SSeCKS expression in rat brain. J Mol Neurosci. 2007;32:9-15 pubmed
    ..All the results suggested that SSeCKS might affect brain development and further research is needed to have a good understanding of its function and mechanism. ..
  7. Daino K, Roch Lefevre S, Ugolin N, Altmeyer Morel S, Guilly M, Chevillard S. Silencing of Cited2 and Akap12 genes in radiation-induced rat osteosarcomas. Biochem Biophys Res Commun. 2009;390:654-8 pubmed publisher
    ..rat OS tumors, Cited2 (Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2) and Akap12 (a kinase anchoring protein, also known as src-suppressed C-kinase substrate, SSeCKS) genes mapped to the most ..
  8. Streb J, Miano J. Cross-species sequence analysis reveals multiple charged residue-rich domains that regulate nuclear/cytoplasmic partitioning and membrane localization of a kinase anchoring protein 12 (SSeCKS/Gravin). J Biol Chem. 2005;280:28007-14 pubmed
    ..sequence analysis and deletion mapping to facilitate the identification of the targeting determinants of AKAP12 (also known as SSeCKS or Gravin)...
  9. Li H, Sun G, Fei L, Shen J. [Studies on the expression of Src-suppressed C kinase substrate mRNA in pulmonary microvascular endothelial cells induced by lipopolysaccharide]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2008;20:65-8 pubmed
    ..This indicate SSeCKS is related with LPS induced injury of RPMVEC. (2) Methylprednisolone sodium succinate inhibit the increase of SSeCKS mRNA induced by LPS. ..

More Information


  1. Chen S, Sun G, You Q, Wang N. [Induction effect of platelet-activating factor on Src-suppressed C kinase substrate gene expression in rat pulmonary microvascular endothelial cells]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010;22:135-8 pubmed
    ..001 9, P>0.05). PAF can up regulate the expression of SSeCKS mRNA in a dose- and time-dependent manner in RPMVECs. It is NF-KappaB rather than PKC signal pathway that is involved in modulation of the intracellular signaling process. ..
  2. Zhou Z, Tao T, Ji Y, Yang H, Wang Y, Cheng C, et al. SSeCKS promotes tumor necrosis factor-alpha autocrine via activating p38 and JNK pathways in Schwann cells. Cell Mol Neurobiol. 2010;30:701-7 pubmed publisher
    ..Such effects might be resulted in SSeCKS promoted p38 and JNK activation in SCs treated by TNF-alpha. Thus present data show that while SCs activation, SSeCKS may plays an important role in the release of inflammatory mediators. ..
  3. Rhim J, Jang I, Yeo E, Song K, Park S. Role of protein kinase C-dependent A-kinase anchoring proteins in lysophosphatidic acid-induced cAMP signaling in human diploid fibroblasts. Aging Cell. 2006;5:451-61 pubmed
  4. Wang P, Sun L, Shen A, Yang J, Li X, Liu H, et al. Involvement of SRC-suppressed C kinase substrate in neuronal death caused by the lipopolysaccharide-induced reactive astrogliosis. Inflammation. 2010;33:359-73 pubmed publisher
    ..This study delineates that SSeCKS may be important for host defenses in spinal inflammation and suggests a valuable molecular mechanism by which astrocytes modify neuronal viability during pathological states. ..
  5. Cheng C, Liu H, Ge H, Qian J, Qin J, Sun L, et al. Lipopolysaccharide induces expression of SSeCKS in rat lung microvascular endothelial cell. Mol Cell Biochem. 2007;305:1-8 pubmed
    ..We conclude that SSeCKS is located downstream of PKC and that SSeCKS and PKC are both necessary for LPS-induced stress fiber formation. ..
  6. You Q, Sun G, Wang N, Shen J, Wang Y. Interleukin-17F-induced pulmonary microvascular endothelial monolayer hyperpermeability via the protein kinase C pathway. J Surg Res. 2010;162:110-21 pubmed publisher
    ..IL-17F is an important mediator of increased endothelial permeability. PKC and SSeCKS are integral signaling components essential for IL-17F-induced hyperpermeability. ..
  7. Li X, Yan M, Hu L, Sun L, Zhang F, Ji H, et al. Involvement of Src-suppressed C kinase substrate in experimental autoimmune encephalomyelitis: a link between release of astrocyte proinflammatory factor and oligodendrocyte apoptosis. J Neurosci Res. 2010;88:1858-71 pubmed publisher
    ..Understanding the mechanism of SSeCKS in the pathogenesis of EAE may contribute to the development of new therapeutic strategies against EAE and MS. ..
  8. Sun L, Cheng C, Liu H, Xiao F, Qin J, Shao X, et al. Changes of Src-suppressed C kinase substrate expression in cytokine induced reactive C6 glioma cells. Neurosci Bull. 2007;23:101-6 pubmed
    ..TNF-alpha activates PKC and upregulates SSeCKS expression in C6 glioma cells. These effects are associated with PKC activity, suggesting that SSeCKS plays a role in response to glia activation in PKC mediated pathway. ..
  9. Liu Y, Gao L, Gelman I. SSeCKS/Gravin/AKAP12 attenuates expression of proliferative and angiogenic genes during suppression of v-Src-induced oncogenesis. BMC Cancer. 2006;6:105 pubmed
    ..Our data provide further evidence that SSeCKS can suppress Src-induced oncogenesis by modulating gene expression downstream of Src kinase activity. ..
  10. Cui Z, Tao T, Cheng C, Yang J, Shen Q, Ji Y, et al. SSeCKS promote beta-amyloid-induced PC12 cells neurotoxicity by up-regulating tau phosphorylation in Alzheimer's disease. Mol Cell Biochem. 2010;340:257-63 pubmed publisher
    ..In summary, our data indicate that SSeCKS may play a critical role in Abeta-induced PC12 cells apoptosis through its phosphorylation. ..
  11. Yan M, Cheng C, Jiang J, Liu Y, Gao Y, Guo Z, et al. Essential role of SRC suppressed C kinase substrates in Schwann cells adhesion, spreading and migration. Neurochem Res. 2009;34:1002-10 pubmed publisher
    ..These results demonstrated SSeCKS modulate Schwann cells adhesion, spreading and migration by reorganization of the actin cytoskeleton. ..
  12. Selvaraju V, Suresh S, Thirunavukkarasu M, Mannu J, Foye J, Mathur P, et al. Regulation of A-Kinase-Anchoring Protein 12 by Heat Shock Protein A12B to Prevent Ventricular Dysfunction Following Acute Myocardial Infarction in Diabetic Rats. J Cardiovasc Transl Res. 2017;10:209-220 pubmed publisher
    ..along with decreased expression of thioredoxin interacting protein (TXNIP) and A kinase anchoring protein 12 (AKAP12) in the DMI-AdHSPA12B compared to DMI-AdLacZ group...
  13. Coats S, Pabón Peña L, Covington J, Vaughan D. Ligand-specific control of src-suppressed C kinase substrate gene expression. Biochem Biophys Res Commun. 2002;297:1112-20 pubmed
    ..In addition, ectopic expression of recombinant SSeCKS recapitulates attributes of NaB-induced morphogenesis in KNRK cells. The data provide novel evidence that SSeCKS functions in PDGF-BB-, LPA-, EPA-, and NaB-mediated cell signaling. ..
  14. Carnegie G, Means C, Scott J. A-kinase anchoring proteins: from protein complexes to physiology and disease. IUBMB Life. 2009;61:394-406 pubmed publisher
    ..These anchoring proteins form multi-protein complexes that integrate cAMP signaling with other pathways and signaling events. In this review, we focus on recent advances in the elucidation of AKAP function. ..
  15. Streb J, Kitchen C, Gelman I, Miano J. Multiple promoters direct expression of three AKAP12 isoforms with distinct subcellular and tissue distribution profiles. J Biol Chem. 2004;279:56014-23 pubmed
    A Kinase Anchoring Protein 12 (AKAP12; also known as src-suppressed C kinase substrate (SSeCKS) and Gravin) is a multivalent anchoring protein with tumor suppressor activity...
  16. Yan M, Zhao J, Zhu S, Shao X, Zhang L, Gao H, et al. Expression of SRC suppressed C kinase substrate in rat neural tissues during inflammation. Neurochem Res. 2014;39:748-57 pubmed publisher
    ..These findings indicated that SSeCKS might play some important roles in sciatic nerve fibers and neurons in spinal cords and dorsal root ganglions after LPS injection. ..
  17. Chapline C, Mousseau B, Ramsay K, Duddy S, Li Y, Kiley S, et al. Identification of a major protein kinase C-binding protein and substrate in rat embryo fibroblasts. Decreased expression in transformed cells. J Biol Chem. 1996;271:6417-22 pubmed
    ..Our results emphasize that the role of PKCs in carcinogenesis may involve several factors, including the quantity and location of the PKCs isozymes and their downstream targets. ..
  18. Xia Y, Liu H, Shen A, Liu Y, Sun L, Tao T, et al. A critical role of SRC-suppressed C kinase substrate in rat astrocytes after chronic constriction injury. Neuromolecular Med. 2010;12:205-16 pubmed publisher
    ..Taken together, activated astrocytes released cytokines and iNOS after neuropathic pain via SSeCKS-ERK signaling. SSeCKS might be critical for the activation of astrocytes in the neuropathic pain. ..
  19. Ji Y, Tao T, Cheng C, Yang H, Wang Y, Yang J, et al. SSeCKS is a suppressor in Schwann cell differentiation and myelination. Neurochem Res. 2010;35:219-26 pubmed publisher
    ..In addition, we found suppression of SSeCKS expression promoted Akt serine 473 phosphorylation in cAMP-treated Schwann cells. In summary, our data indicated that SSeCKS was a negative regulator of myelinating glia differentiation. ..
  20. Chen L, Qin J, Cheng C, Niu S, Liu Y, Shi S, et al. Spatiotemporal expression of SSeCKS in injured rat sciatic nerve. Anat Rec (Hoboken). 2008;291:527-37 pubmed publisher
    ..These findings support the idea that SSeCKS participates in the adaptive response to peripheral nerve injury and may be associated with regeneration. ..
  21. You Q, Sun G, Wang N, Chen S, Luo Q. Role of src-suppressed C kinase substrate in rat pulmonary microvascular endothelial hyperpermeability stimulated by inflammatory cytokines. Inflamm Res. 2010;59:949-58 pubmed publisher
    ..PKC inhibitors also significantly decreased cytokine-induced hyperpermeability and SSeCKS expression. SSeCKS is involved in the endothelial hyperpermeability induced by IL-1? and TNF-? in inflammatory process. ..
  22. Xia W, Gelman I. Mitogen-induced, FAK-dependent tyrosine phosphorylation of the SSeCKS scaffolding protein. Exp Cell Res. 2002;277:139-51 pubmed
    ..These data suggest that mitogen-induced, FAK-dependent tyrosine phosphorylation of SSeCKS modulates its binding to the actin-based cytoskeleton, suggesting a role for SSeCKS in mitogen-induced cytoskeletal reorganization. ..
  23. Sun L, Cheng C, Liu H, Shen C, Xiao F, Qin J, et al. Src suppressed C kinase substrate regulates the lipopolysaccharide-induced TNF-alpha biosynthesis in rat astrocytes. J Mol Neurosci. 2007;32:16-24 pubmed
    ..These results suggest that SSeCKS is involved in the LPS-induced TNF-alpha expression in astrocytes mediated by PKC. ..