Gene Symbol: Afdn
Description: afadin, adherens junction formation factor
Alias: Af6, Mllt4, afadin, myeloid/lymphoid or mixed lineage-leukemia translocation to 4 homolog, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4, myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4, protein Af-6, translocated to, 4
Species: rat
Products:     Afdn

Top Publications

  1. Takahashi K, Nakanishi H, Miyahara M, Mandai K, Satoh K, Satoh A, et al. Nectin/PRR: an immunoglobulin-like cell adhesion molecule recruited to cadherin-based adherens junctions through interaction with Afadin, a PDZ domain-containing protein. J Cell Biol. 1999;145:539-49 pubmed
    We have isolated a novel actin filament-binding protein, named afadin, localized at cadherin-based cell-cell adherens junctions (AJs) in various tissues and cell lines...
  2. Buchert M, Poon C, King J, Baechi T, D Abaco G, Hollande F, et al. AF6/s-afadin is a dual residency protein and localizes to a novel subnuclear compartment. J Cell Physiol. 2007;210:212-23 pubmed
    The AF6/afadin protein is a component of cell membranes at specialized sites of cell-cell contact. Two main splice variants exist, known as l- and s-afadin, respectively...
  3. Hock B, Böhme B, Karn T, Yamamoto T, Kaibuchi K, Holtrich U, et al. PDZ-domain-mediated interaction of the Eph-related receptor tyrosine kinase EphB3 and the ras-binding protein AF6 depends on the kinase activity of the receptor. Proc Natl Acad Sci U S A. 1998;95:9779-84 pubmed
    ..the Eph-related RTKs EphA7, EphB2, EphB3, EphB5, and EphB6 interact with the PDZ domain of the ras-binding protein AF6. A mutational analysis revealed that six C-terminal residues of the receptors are involved in binding to the PDZ ..
  4. Xie Z, Huganir R, Penzes P. Activity-dependent dendritic spine structural plasticity is regulated by small GTPase Rap1 and its target AF-6. Neuron. 2005;48:605-18 pubmed
    ..These results identify a signaling pathway that regulates activity-dependent synaptic structural plasticity and coordinates it with functional plasticity. ..
  5. Mandai K, Nakanishi H, Satoh A, Obaishi H, Wada M, Nishioka H, et al. Afadin: A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherens junction. J Cell Biol. 1997;139:517-28 pubmed
    ..We named p205 l-afadin (a large splicing variant of AF-6 protein localized at adherens junction) and p190 s-afadin (a small splicing ..
  6. Kawabe H, Hata Y, Takeuchi M, Ide N, Mizoguchi A, Takai Y. nArgBP2, a novel neural member of ponsin/ArgBP2/vinexin family that interacts with synapse-associated protein 90/postsynaptic density-95-associated protein (SAPAP). J Biol Chem. 1999;274:30914-8 pubmed
    ..nArgBP2 bound to not only SAPAP but also vinculin and l-afadin, known to bind to ponsin and vinexin. nArgBP2 may be implicated in the protein network around SAPAP in the PSD.
  7. Nojima H, Adachi M, Matsui T, Okawa K, Tsukita S, Tsukita S. IQGAP3 regulates cell proliferation through the Ras/ERK signalling cascade. Nat Cell Biol. 2008;10:971-8 pubmed publisher
    ..Thus, IQGAP3 regulates the promotion of cell proliferation through Ras-dependent ERK activation. ..
  8. Pantaleon M, Kanai Azuma M, Mattick J, Kaibuchi K, Kaye P, Wood S. FAM deubiquitylating enzyme is essential for preimplantation mouse embryo development. Mech Dev. 2001;109:151-60 pubmed
    ..However, the nascent protein was mislocalised at the apical surface of blastomeres. Therefore FAM is required for preimplantation mouse embryo development and regulates beta-catenin and AF-6 in vivo. ..
  9. Tachibana K, Nakanishi H, Mandai K, Ozaki K, Ikeda W, Yamamoto Y, et al. Two cell adhesion molecules, nectin and cadherin, interact through their cytoplasmic domain-associated proteins. J Cell Biol. 2000;150:1161-76 pubmed
    ..cell-cell adhesion system at cadherin-based cell-cell adherens junctions (AJs) consisting of at least nectin and l-afadin. Nectin is a Ca(2+)-independent homophilic immunoglobulin-like adhesion molecule, and l-afadin is an actin ..

More Information


  1. Rehm K, Panzer L, van Vliet V, Genot E, Linder S. Drebrin preserves endothelial integrity by stabilizing nectin at adherens junctions. J Cell Sci. 2013;126:3756-69 pubmed publisher
    ..stabilization occurs through a chain of interactions: drebrin binding to F-actin, interaction of drebrin and afadin through their polyproline and PR1-2 regions, and recruitment of nectin through the PDZ region of afadin...
  2. Fukumoto Y, Kurita S, Takai Y, Ogita H. Role of scaffold protein afadin dilute domain-interacting protein (ADIP) in platelet-derived growth factor-induced cell movement by activating Rac protein through Vav2 protein. J Biol Chem. 2011;286:43537-48 pubmed publisher
    ..We show here that the scaffold protein afadin dilute domain-interacting protein (ADIP), an afadin-binding protein, is involved in the regulation of cell ..
  3. Méndez J, De Haro Hernández R, Conejo V. Spermine increases arginase activity in the liver after carbon tetrachloride-induced hepatic injury in Long-Evans rats. Biomed Pharmacother. 2006;60:82-5 pubmed
  4. Manabe N, Hirai S, Imai F, Nakanishi H, Takai Y, Ohno S. Association of ASIP/mPAR-3 with adherens junctions of mouse neuroepithelial cells. Dev Dyn. 2002;225:61-9 pubmed
    ..on tight junctions in cultured epithelial cells, it localizes on adherens junctions outlined by beta-catenin and afadin at the luminal surface, an apical end of the neuroepithelium in developing mouse central nervous systems...
  5. VanLeeuwen J, Rafalovich I, Sellers K, Jones K, Griffith T, Huda R, et al. Coordinated nuclear and synaptic shuttling of afadin promotes spine plasticity and histone modifications. J Biol Chem. 2014;289:10831-42 pubmed publisher
    ..that activation of NMDA receptors induces the bi-directional and concomitant shuttling of the scaffold protein afadin from the cytosol to the nucleus and synapses...
  6. Inagaki M, Irie K, Ishizaki H, Tanaka Okamoto M, Morimoto K, Inoue E, et al. Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development. Development. 2005;132:1525-37 pubmed
  7. Umeda K, Iwasawa N, Negishi M, Oinuma I. A short splicing isoform of afadin suppresses the cortical axon branching in a dominant-negative manner. Mol Biol Cell. 2015;26:1957-70 pubmed publisher
    ..We recently identified afadin, an F-actin-binding protein, as an effector of R-Ras mediating axon branching through F-actin reorganization...
  8. Haskin J, Szargel R, Shani V, Mekies L, Rott R, Lim G, et al. AF-6 is a positive modulator of the PINK1/parkin pathway and is deficient in Parkinson's disease. Hum Mol Genet. 2013;22:2083-96 pubmed publisher
    ..The identification of AF-6 as a positive modulator of parkin translocation to the mitochondria sheds light on the mechanisms involved in PD and underscores AF-6 as a novel target for future therapeutics. ..
  9. Kanai Azuma M, Mattick J, Kaibuchi K, Wood S. Co-localization of FAM and AF-6, the mammalian homologues of Drosophila faf and canoe, in mouse eye development. Mech Dev. 2000;91:383-6 pubmed
    ..Expression is not limited to the epithelia however, as FAM and AF-6 also co-localize during lens fibre development as well as in sub-populations of the neural retina. ..
  10. Ooshio T, Irie K, Morimoto K, Fukuhara A, Imai T, Takai Y. Involvement of LMO7 in the association of two cell-cell adhesion molecules, nectin and E-cadherin, through afadin and alpha-actinin in epithelial cells. J Biol Chem. 2004;279:31365-73 pubmed
    ..recruit cadherins to the nectin-based cell-cell adhesion sites through their cytoplasm-associated proteins, afadin and catenins, it is not fully understood how nectins are physically associated with cadherins...
  11. Srivastava D, Copits B, Xie Z, Huda R, Jones K, Mukherji S, et al. Afadin is required for maintenance of dendritic structure and excitatory tone. J Biol Chem. 2012;287:35964-74 pubmed publisher
    ..In this study, we find that afadin, a component of N-cadherin·?-catenin·?-N-catenin adhesion complexes, is required for the maintenance of ..
  12. Gil Sanz C, Landeira B, Ramos C, Costa M, M ller U. Proliferative defects and formation of a double cortex in mice lacking Mltt4 and Cdh2 in the dorsal telencephalon. J Neurosci. 2014;34:10475-87 pubmed publisher
    ..Here we show that the adherens junction proteins afadin and CDH2 are critical for the control of cell proliferation in the dorsal telencephalon and for the formation of ..
  13. Nakano K, Matuda S, Yamanaka T, Tsubouchi H, Nakagawa S, Titani K, et al. Purification and molecular cloning of succinyltransferase of the rat alpha-ketoglutarate dehydrogenase complex. Absence of a sequence motif of the putative E3 and/or E1 binding site. J Biol Chem. 1991;266:19013-7 pubmed
    ..These results show that the rat succinyltransferase does not have the sequence motif of the putative E3- and/or E1-binding site. ..
  14. Sato T, Kanai Y, Noma T, Kanai Azuma M, Taya S, Matsui T, et al. A close correlation in the expression patterns of Af-6 and Usp9x in Sertoli and granulosa cells of mouse testis and ovary. Reproduction. 2004;128:583-94 pubmed
    ..It further suggests that the post-translational regulation of Af-6 by Usp9x may be one potential pathway to control the cell adhesion dynamics in mammalian gametogenesis. ..
  15. Takekuni K, Ikeda W, Fujito T, Morimoto K, Takeuchi M, Monden M, et al. Direct binding of cell polarity protein PAR-3 to cell-cell adhesion molecule nectin at neuroepithelial cells of developing mouse. J Biol Chem. 2003;278:5497-500 pubmed
    ..Nectins are associated with the actin cytoskeleton through afadin, of which the PDZ domain binds to nectins through their C-terminal four amino acids...
  16. Ebnet K, Schulz C, Meyer Zu Brickwedde M, Pendl G, Vestweber D. Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1. J Biol Chem. 2000;275:27979-88 pubmed
    ..Our results suggest that JAM can be recruited to intercellular junctions by its interaction with the PDZ domain-containing proteins AF-6 and possibly ZO-1. ..
  17. Ishiuchi T, Takeichi M. Nectins localize Willin to cell-cell junctions. Genes Cells. 2012;17:387-97 pubmed publisher
    ..In addition, we report that the positioning of nectins at the AJC is dependent on their binding to afadin. Thus, our results suggest that the nectin-afadin interaction plays a role in the correct localization of Willin.
  18. Asada M, Irie K, Morimoto K, Yamada A, Ikeda W, Takeuchi M, et al. ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. J Biol Chem. 2003;278:4103-11 pubmed
    b>Afadin is an actin filament (F-actin)-binding protein that is associated with the cytoplasmic tail of nectin, a Ca(2+)-independent immunoglobulin-like cell-cell adhesion molecule...