Gene Symbol: acrosin
Description: acrosin
Alias: Acro, acrosin, acrosin prepropeptide
Species: rat
Products:     acrosin

Top Publications

  1. NagDas S, Skudlarek M, Orgebin Crist M, Tulsiani D. Biochemical alterations in the proacrosin-acrosin system during epididymal maturation of the rat spermatozoa. J Androl. 1992;13:36-43 pubmed
    b>Acrosin, an acrosomal serine protease, is believed to have a role in fertilization. The enzyme is synthesized in an enzymatically inactive precursor form, proacrosin, and is processed to enzymatically active form(s)...
  2. Isotani A, Matsumura T, Ogawa M, Tanaka T, Yamagata K, Ikawa M, et al. A delayed sperm penetration of cumulus layers by disruption of acrosin gene in rats. Biol Reprod. 2017;97:61-68 pubmed publisher
    b>Acrosin, the trypsin-like serine protease in the sperm acrosome, was long viewed as a key enzyme required for zona pellucida penetration to fertilize eggs...
  3. Zheng X, Geiger M, Ecke S, Bielek E, Donner P, Eberspächer U, et al. Inhibition of acrosin by protein C inhibitor and localization of protein C inhibitor to spermatozoa. Am J Physiol. 1994;267:C466-72 pubmed
    ..We analyzed the interaction of PCI with acrosin, a serine protease stored in its zymogen form in the acrosome of spermatozoa...
  4. Klemm U, Flake A, Engel W. Rat sperm acrosin: cDNA sequence, derived primary structure and phylogenetic origin. Biochim Biophys Acta. 1991;1090:270-2 pubmed like the catalytic triad (His-70, Asp-124, Ser-222) are highly conserved not only between the available acrosin primary structures of different mammals but also in comparison with other serine proteinases...
  5. Lister P. Implementing Project 2000 in a context of change. Sr Nurse. 1992;12:34-6 pubmed
  6. Nayernia K, Drabent B, Meinhardt A, Adham I, Schwandt I, Muller C, et al. Triple knockouts reveal gene interactions affecting fertility of male mice. Mol Reprod Dev. 2005;70:406-16 pubmed
    ..These findings demonstrate that male fertility involves synergistic interactions of genes that function in sperm motility and sperm-egg adhesion during fertilization. ..
  7. Elce J, McIntyre E. Purification of bovine and human acrosin. Can J Biochem. 1982;60:8-14 pubmed
    b>Acrosin from human spermatozoa was required for studies of immunological interference with fertilization, but not detailed purification scheme was available for the human enzyme...
  8. Yudin A, VandeVoort C, Li M, Overstreet J. PH-20 but not acrosin is involved in sperm penetration of the macaque zona pellucida. Mol Reprod Dev. 1999;53:350-62 pubmed
    In this study, we investigated the functions of PH-20 and acrosin during the interaction of macaque sperm with the zona pellucida...
  9. Adham I, Nayernia K, Engel W. Spermatozoa lacking acrosin protein show delayed fertilization. Mol Reprod Dev. 1997;46:370-6 pubmed
    b>Acrosin (ACR), a serine proteinase located in the acrosome of the sperm, has been presumed to be involved in the recognition and binding of the sperm to the zona pellucida of the ovum and the sperm penetration through the zona pellucida...

Scientific Experts

More Information


  1. Baba T, Azuma S, Kashiwabara S, Toyoda Y. Sperm from mice carrying a targeted mutation of the acrosin gene can penetrate the oocyte zona pellucida and effect fertilization. J Biol Chem. 1994;269:31845-9 pubmed
    The physiological function of mammalian sperm acrosin has long been believed to be involved in the limited proteolysis of the oocyte zona pellucida, thus enabling the sperm to penetrate this extracellular matrix and to gain access to the ..
  2. Martinez Menarguez J, Geuze H, Ballesta J. Evidence for a nonlysosomal origin of the acrosome. J Histochem Cytochem. 1996;44:313-20 pubmed
    ..cryosections of rat testis, using a variety of antibodies against endosomal/lysosomal marker protein and acrosin, the major secretory protein of sperm cells...
  3. Nayernia K, Reim K, Oberwinkler H, Engel W. Diploid expression and translational regulation of rat acrosin gene. Biochem Biophys Res Commun. 1994;202:88-93 pubmed
    b>Acrosin, a sperm acrosomal serine protease has been implicated in the recognition, binding and penetration of the zona pellucida of the ovum...
  4. Nayernia K, Nieter S, Kremling H, Oberwinkler H, Engel W. Functional and molecular characterization of the transcriptional regulatory region of the proacrosin gene. J Biol Chem. 1994;269:32181-6 pubmed
    Proacrosin, the zymogen form of the serine protease acrosin, is located within the acrosomal vesicle of mammalian spermatozoa and has been suggested to be involved in the fertilization process...
  5. Nayernia K, Meinhardt A, Drabent B, Adham I, Muller C, Steckel M, et al. Synergistic effects of germ cell expressed genes on male fertility in mice. Cytogenet Genome Res. 2003;103:314-20 pubmed
    ..Proacrosin is an acrosomal protease synthesized as a proenzyme and activated into acrosin during the acrosome reaction. The linker histone subtype H1...
  6. Chen J, Menesini Chen M, Giachetti D, Matteucci F, Barbetti M, Sensini C, et al. Correlation between male fertility and acrosin-like protease activity in rats treated with Spartium junceum. Zygote. 1993;1:309-13 pubmed
    ..injections of extracts of Spartium junceum in adult male rats were shown to reduce the rate of fertility and acrosin enzyme activity...
  7. Rao M, Shah K. Endocrine approach to male fertility control by steroid hormone combination in rat Rattus norvegicus L. Indian J Exp Biol. 1998;36:775-9 pubmed
    ..Sperm acrosomal enzymes such as acrosin and hyaluronidase were also affected leading to a loss of their function...
  8. Kremling H, Flake A, Adham I, Radtke J, Engel W. Exon-intron structure and nucleotide sequence of the rat proacrosin gene. DNA Seq. 1991;2:57-60 pubmed
    ..The transcription initiation site was determined at position 471 (564 nucleotides upstream of ATG), TATA- and TAAT-boxes were found 588 and 656 bp upstream of the ATG-translation start point, respectively. ..
  9. De Jonge C, Mack S, Zaneveld L. Inhibition of the human sperm acrosome reaction by proteinase inhibitors. Gamete Res. 1989;23:387-97 pubmed
    ..proteinase inhibitor 4'-acetamidophenyl 4-guanidinobenzoate (AGB), an inhibitor of proacrosin activation and of acrosin, when added at either the onset of incubation or to capacitated spermatozoa, 5 min prior to stimulation by dbcAMP,..
  10. Honda A, Siruntawineti J, Baba T. Role of acrosomal matrix proteases in sperm-zona pellucida interactions. Hum Reprod Update. 2002;8:405-12 pubmed
    ..Of the acrosomal proteases identified, acrosin exhibits two distinct activities: (i) an enzymatic activity as a trypsin-like serine protease; and (ii) a lectin-..
  11. Furlong L, Veaute C, Vazquez Levin M. Binding of recombinant human proacrosin/acrosin to zona pellucida glycoproteins. II. Participation of mannose residues in the interaction. Fertil Steril. 2005;83:1791-6 pubmed
    To assess the interaction of human proacrosin/acrosin with mannose residues coupled to a protein backbone. Prospective study. Basic research laboratory...
  12. Johnson R, Jakobs K, Schultz G. Extraction of the adenylate cyclase-activating factor of bovine sperm and its identification as a trypsin-like protease. J Biol Chem. 1985;260:114-21 pubmed
    ..alpha-Chymotrypsin, trypsin, and sperm acrosin were comparably effective in stimulating the platelet adenylate cyclase 5- to 8-fold, with concentrations ..