3 alpha hydroxysteroid dehydrogenase

Summary

Gene Symbol: 3 alpha hydroxysteroid dehydrogenase
Description: aldo-keto reductase family 1, member C14
Alias: Akr1c9, 3-alpha-hydroxysteroid dehydrogenase, 3-alpha-HSD, hydroxyprostaglandin dehydrogenase, steroid 3-alpha-dehydrogenase
Species: rat

Top Publications

  1. ncbi Elucidation of a complete kinetic mechanism for a mammalian hydroxysteroid dehydrogenase (HSD) and identification of all enzyme forms on the reaction coordinate: the example of rat liver 3alpha-HSD (AKR1C9)
    William C Cooper
    Center of Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6084, USA
    J Biol Chem 282:33484-93. 2007
  2. ncbi Isolation and partial characterization of a full-length cDNA clone for 3 alpha-hydroxysteroid dehydrogenase: a potential target enzyme for nonsteroidal anti-inflammatory drugs
    J Pawlowski
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104 6084
    Agents Actions 34:289-93. 1991
  3. doi Molecular cloning of rat liver 3α-hydroxysteroid dehydrogenase and identification of structurally related proteins from rat lung and kidney
    K C Cheng
    Department of Pediatrics, Cornell University Medical College, New York, NY 10021, USA
    J Steroid Biochem Mol Biol 43:1083-8. 1992
  4. ncbi Anatomical and cellular localization of neuroactive 5 alpha/3 alpha-reduced steroid-synthesizing enzymes in the spinal cord
    Christine Patte-Mensah
    Laboratoire de Neurophysiologie Cellulaire et Intégrée, Unité Mixte de Recherche 7519 Centre National de la Recherche Scientifique, Universite Louis Pasteur, 67084 Strasbourg, France
    J Comp Neurol 477:286-99. 2004
  5. ncbi Structure of 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase complexed with NADP+
    M J Bennett
    Department of Biochemistry and Biophysics, Johnson Research Foundation, University of Pennsylvania School of Medicine, Philadelphia 19104 6059, USA
    Biochemistry 35:10702-11. 1996
  6. ncbi Rat hepatic 3 alpha-hydroxysteroid dehydrogenase: expression of cDNA and physiological function in bile acid biosynthetic pathway
    E Usui
    Department of Biochemistry, Hiroshima University School of Dentistry
    J Biochem 115:230-7. 1994
  7. ncbi Distribution of 3 alpha-hydroxysteroid dehydrogenase in rat brain and molecular cloning of multiple cDNAs encoding structurally related proteins in humans
    M Khanna
    Department of Pediatrics, Cornell University Medical College, New York, NY 10021, USA
    J Steroid Biochem Mol Biol 53:41-6. 1995
  8. pmc Purification of NADPH-dependent dehydroascorbate reductase from rat liver and its identification with 3 alpha-hydroxysteroid dehydrogenase
    B Del Bello
    Instituto di Patologia Generale, Universita di Siena, Italy
    Biochem J 304:385-90. 1994
  9. pmc Three-dimensional structure of rat liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase: a member of the aldo-keto reductase superfamily
    S S Hoog
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104 6084
    Proc Natl Acad Sci U S A 91:2517-21. 1994
  10. ncbi Affinity labeling of 3 alpha-hydroxysteroid dehydrogenase with 3 alpha-bromoacetoxyandrosterone and 11 alpha-bromoacetoxyprogesterone. Isolation and sequence of active site peptides containing reactive cysteines; sequence confirmation using nucleotide seq
    T M Penning
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104
    J Biol Chem 266:8826-34. 1991

Detail Information

Publications17

  1. ncbi Elucidation of a complete kinetic mechanism for a mammalian hydroxysteroid dehydrogenase (HSD) and identification of all enzyme forms on the reaction coordinate: the example of rat liver 3alpha-HSD (AKR1C9)
    William C Cooper
    Center of Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6084, USA
    J Biol Chem 282:33484-93. 2007
    ..the complete kinetic mechanism of a mammalian HSD using rat 3alpha-HSD of the aldo-keto reductase superfamily (AKR1C9) with the substrate pairs androstane-3,17-dione and NADPH (reduction) and androsterone and NADP(+) (oxidation)...
  2. ncbi Isolation and partial characterization of a full-length cDNA clone for 3 alpha-hydroxysteroid dehydrogenase: a potential target enzyme for nonsteroidal anti-inflammatory drugs
    J Pawlowski
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104 6084
    Agents Actions 34:289-93. 1991
    ..cytosol is a monomeric (Mr 34000) NAD(P)+ dependent oxidoreductase which displays 9-, 11- & 15-hydroxyprostaglandin dehydrogenase activity...
  3. doi Molecular cloning of rat liver 3α-hydroxysteroid dehydrogenase and identification of structurally related proteins from rat lung and kidney
    K C Cheng
    Department of Pediatrics, Cornell University Medical College, New York, NY 10021, USA
    J Steroid Biochem Mol Biol 43:1083-8. 1992
    ....
  4. ncbi Anatomical and cellular localization of neuroactive 5 alpha/3 alpha-reduced steroid-synthesizing enzymes in the spinal cord
    Christine Patte-Mensah
    Laboratoire de Neurophysiologie Cellulaire et Intégrée, Unité Mixte de Recherche 7519 Centre National de la Recherche Scientifique, Universite Louis Pasteur, 67084 Strasbourg, France
    J Comp Neurol 477:286-99. 2004
    ..These results are the first demonstrating that oligodendrocytes and neurons of the SC possess the key enzymatic complex for synthesizing potent neuroactive steroids that may control spinal sensorimotor processes...
  5. ncbi Structure of 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase complexed with NADP+
    M J Bennett
    Department of Biochemistry and Biophysics, Johnson Research Foundation, University of Pennsylvania School of Medicine, Philadelphia 19104 6059, USA
    Biochemistry 35:10702-11. 1996
    ..Knowledge of the position of this water molecule, combined with the stereochemistry of hydride transfer, suggests that the alpha face of a bound steroid will be oriented toward the side of the apolar cleft containing Trp 86...
  6. ncbi Rat hepatic 3 alpha-hydroxysteroid dehydrogenase: expression of cDNA and physiological function in bile acid biosynthetic pathway
    E Usui
    Department of Biochemistry, Hiroshima University School of Dentistry
    J Biochem 115:230-7. 1994
    ..abstract truncated at 250 words)..
  7. ncbi Distribution of 3 alpha-hydroxysteroid dehydrogenase in rat brain and molecular cloning of multiple cDNAs encoding structurally related proteins in humans
    M Khanna
    Department of Pediatrics, Cornell University Medical College, New York, NY 10021, USA
    J Steroid Biochem Mol Biol 53:41-6. 1995
    ..We are currently investigating the functional relationship between the enzymes encoded by these human cDNAs and 3 alpha-hydroxysteroid dehydrogenase...
  8. pmc Purification of NADPH-dependent dehydroascorbate reductase from rat liver and its identification with 3 alpha-hydroxysteroid dehydrogenase
    B Del Bello
    Instituto di Patologia Generale, Universita di Siena, Italy
    Biochem J 304:385-90. 1994
    ..Internal primary structure data were obtained from CNBr-derived fragments and definitely proved the identity of NADPH-dependent DHAA reductase with 3 alpha-hydroxysteroid dehydrogenase...
  9. pmc Three-dimensional structure of rat liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase: a member of the aldo-keto reductase superfamily
    S S Hoog
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104 6084
    Proc Natl Acad Sci U S A 91:2517-21. 1994
    ..In the 3 alpha-HSD structure these residues are on the periphery of the barrel and are unlikely to participate in catalysis...
  10. ncbi Affinity labeling of 3 alpha-hydroxysteroid dehydrogenase with 3 alpha-bromoacetoxyandrosterone and 11 alpha-bromoacetoxyprogesterone. Isolation and sequence of active site peptides containing reactive cysteines; sequence confirmation using nucleotide seq
    T M Penning
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104
    J Biol Chem 266:8826-34. 1991
    ..By contrast the 11 alpha-bromoacetoxy group in Br11P labeled several reactive cysteines which may be involved in the binding of glucocorticoids and NSAIDs...
  11. ncbi Genomic structure of rat 3alpha-hydroxysteroid/dihydrodiol dehydrogenase (3alpha-HSD/DD, AKR1C9)
    H K Lin
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104 6084, USA
    J Steroid Biochem Mol Biol 71:29-39. 1999
    ..It is unknown whether the type 2 and type 3 3alpha-HSD/DD genes represent pseudo-genes or whether they represent genes that are differentially expressed in other rat tissues...
  12. ncbi Alanine scanning mutagenesis of the testosterone binding site of rat 3 alpha-hydroxysteroid dehydrogenase demonstrates contact residues influence the rate-determining step
    Vladi V Heredia
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6084, USA
    Biochemistry 43:5832-41. 2004
    ..and steroid turnover by these enzymes were analyzed using rat 3alpha-hydroxysteroid dehydrogenase (3alpha-HSD, AKR1C9) as the protein model...
  13. ncbi Molecular cloning and expression of rat liver 3 alpha-hydroxysteroid dehydrogenase
    K C Cheng
    Department of Pediatrics Cornell University Medical College, New York, New York 10021
    Mol Endocrinol 5:823-8. 1991
    Complementary DNA clones encoding 3 alpha-hydroxysteroid dehydrogenase (3 alpha HSD) were isolated from a rat liver cDNA lambda gt11 expression library using monoclonal antibodies as probes. The sizes of the cDNA inserts ranged from 1...
  14. ncbi Dissection of the physiological interconversion of 5alpha-DHT and 3alpha-diol by rat 3alpha-HSD via transient kinetics shows that the chemical step is rate-determining: effect of mutating cofactor and substrate-binding pocket residues on catalysis
    Vladi V Heredia
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6084, USA
    Biochemistry 43:12028-37. 2004
    ..To identify the rate-determining step in this physiologically important reaction, rat liver 3alpha-HSD (AKR1C9) was used as the protein model for the human homologues in fluorescence stopped-flow transient kinetic and kinetic ..
  15. ncbi Cloning and sequencing of the cDNA for rat liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase
    J E Pawlowski
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104 6084
    J Biol Chem 266:8820-5. 1991
    ..It is identical to dihydrodiol dehydrogenase and it can function as a 9-, 11-, and 15-hydroxyprostaglandin dehydrogenase. Its reactions are potently inhibited by the nonsteroidal anti-inflammatory drugs (NSAIDs)...
  16. doi Semicomprehensive analysis of the postnatal age-related changes in the mRNA expression of sex steroidogenic enzymes and sex steroid receptors in the male rat hippocampus
    Tetsuya Kimoto
    Department of Biophysics and Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo 153 8902, Japan
    Endocrinology 151:5795-806. 2010
    ..These results suggest that the hippocampal sex steroidogenic properties are substantially altered during the postnatal development processes, which might contribute to brain sexual maturation...
  17. ncbi Molecular structure of rat hepatic 3 alpha-hydroxysteroid dehydrogenase. A member of the oxidoreductase gene family
    A Stolz
    Division of Gastrointestinal and Liver Diseases, University of Southern California School of Medicine 90033
    J Biol Chem 266:15253-7. 1991
    ..The significant homology of 3 alpha-HSD with both prostaglandin F synthetase and aldose reductases suggest a subdivision of monomeric, NADPH reductases within the larger oxidoreductases superfamily...