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Genomes and Genes | RAD18SummaryGene Symbol: RAD18 Description: Rad18p Species: Top Publications
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The RAD6 DNA damage tolerance pathway operates uncoupled from the replication fork and is functional beyond S phaseGeorgios I Karras
Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Cell 141:255-67. 2010..We therefore propose that the RAD6 pathway acts on single-stranded gaps left behind newly restarted replication forks...- Activation of ubiquitin-dependent DNA damage bypass is mediated by replication protein aAdelina A Davies
Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK
Mol Cell 29:625-36. 2008..We found that RPA directly interacts with the ubiquitin ligase responsible for the modification of PCNA, Rad18, both in yeast and in mammalian cells... - Ubiquitylation of the 9-1-1 checkpoint clamp is independent of rad6-rad18 and DNA damageAdelina A Davies
Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK
Cell 141:1080-7. 2010A recent report proposed a function of the ubiquitin conjugation factors Rad6 and Rad18 comparable to the bacterial SOS response, controlling damage-induced transcriptional activation and contributing to checkpoint signaling... - Control of spontaneous and damage-induced mutagenesis by SUMO and ubiquitin conjugationPhilipp Stelter
Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043 Marburg, Germany
Nature 425:188-91. 2003..Our findings assign a function to SUMO during S phase and demonstrate how ubiquitin and SUMO, by regulating the accuracy of replication and repair, contribute to overall genomic stability... - Mechanistic analysis of PCNA poly-ubiquitylation by the ubiquitin protein ligases Rad18 and Rad5Joanne L Parker
Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms, Herts, UK
EMBO J 28:3657-66. 2009..analysed the mechanism of poly-ubiquitylation of the replication clamp PCNA by two cooperating E2-E3 pairs, Rad6-Rad18 and Ubc13-Mms2-Rad5... - Saccharomyces cerevisiae MGS1 is essential in strains deficient in the RAD6-dependent DNA damage tolerance pathwayTakashi Hishida
Department of Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3 1, Suita, Osaka 565 0871, Japan
EMBO J 21:2019-29. 2002..We found that mgs1 is synthetic lethal with rad6 and exhibits a synergistic growth defect with rad18 and rad5, which are members of the RAD6 epistasis group important for tolerance of DNA damage during DNA .. - Mgs1 and Rad18/Rad5/Mms2 are required for survival of Saccharomyces cerevisiae mutants with novel temperature/cold sensitive alleles of the DNA polymerase delta subunit, Pol31Niloofar Davoodi Vijeh Motlagh
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Sendai, Miyagi 980 8578, Japan
DNA Repair (Amst) 5:1459-74. 2006..Surprisingly, deletions of RAD18 and MGS1 aggravated the temperature sensitivity conferred by most ts or cs alleles and specifically suppressed the .. - SUMOylation regulates Rad18-mediated template switchDana Branzei
IFOM, the FIRC Institute for Molecular Oncology Foundation, IFOM IEO Campus, Via Adamello 16, 20139 Milan, Italy
Nature 456:915-20. 2008..Gap-filling repair requires homologous recombination as well as Rad18- and Rad5-mediated proliferating cell nuclear antigen (PCNA) polyubiquitylation... - Regulation of gross chromosomal rearrangements by ubiquitin and SUMO ligases in Saccharomyces cerevisiaeAkira Motegi
Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Building 49, Room 4A22, Bethesda, MD 20892, USA
Mol Cell Biol 26:1424-33. 2006..Previously, we showed that inactivation of Rad5 or Rad18, ubiquitin ligases (E3) targeting for proliferating cell nuclear antigen (PCNA), increases the de novo telomere .. - The Saccharomyces cerevisiae Rad6 postreplication repair and Siz1/Srs2 homologous recombination-inhibiting pathways process DNA damage that arises in asf1 mutantsEllen S Kats
Ludwig Institute for Cancer Research, Departments of Medicine and Cellular and Molecular Medicine, and Biomedical Sciences Graduate Program, UC San Diego School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093 0669, USA
Mol Cell Biol 29:5226-37. 2009..Our results show that ASF1 probably contributes to the maintenance of genome stability through multiple mechanisms, some of which involve the PRR and HRS pathways... - RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMOCarsten Hoege
Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany
Nature 419:135-41. 2002..enzymes RAD6 and the MMS2-UBC13 heterodimer, which are recruited to chromatin by the RING-finger proteins RAD18 and RAD5, respectively... - The post-replication repair RAD18 and RAD6 genes are involved in the prevention of spontaneous mutations caused by 7,8-dihydro-8-oxoguanine in Saccharomyces cerevisiaeMarcelo de Padula
CEA, Departement de Radiobiologie et Radiopathologie, UMR217 CNRS Radiobiologie Moléculaire et Cellulaire, BP6, 92265 Fontenay aux Roses, France
Nucleic Acids Res 32:5003-10. 2004..In the present study, we show the RAD18 and RAD6 genes that are required to initiate post-replication repair (PRR) are also involved in the prevention of .. - SUMO modification of PCNA is controlled by DNAJoanne L Parker
Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, UK
EMBO J 27:2422-31. 2008..Instead, the stimulatory effect of DNA on conjugation is mainly attributable to DNA binding of PCNA itself. These findings imply a change in the properties of PCNA upon loading that enhances its capacity to be sumoylated... - Genetic analysis of ionizing radiation-induced mutagenesis in Saccharomyces cerevisiae reveals TransLesion Synthesis (TLS) independent of PCNA K164 SUMOylation and ubiquitinationClark C Chen
Department of Neurosurgery, Massachusetts General Hospital, 55 Fruit St, Boston, MA 02114, USA
DNA Repair (Amst) 5:1475-88. 2006..Further analysis of a mutant simultaneously defective in SUMOylation and mono-ubiquitination (rad18 siz1) revealed that these modifications redundantly affected TLS as well as NHEJ... - Functional and physical interaction of yeast Mgs1 with PCNA: impact on RAD6-dependent DNA damage toleranceTakashi Hishida
Genome Dynamics Group, Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3 1, Suita, Osaka 565 0871, Japan
Mol Cell Biol 26:5509-17. 2006..We also show that PCNA sumoylation inhibits the growth of mgs1 rad18 double mutants, in which PCNA sumoylation and the Srs2 DNA helicase coordinately prevent RAD52-dependent .. - Architecture and assembly of poly-SUMO chains on PCNA in Saccharomyces cerevisiaeHanna Windecker
Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK
J Mol Biol 376:221-31. 2008.... - Crosstalk between SUMO and ubiquitin on PCNA is mediated by recruitment of the helicase Srs2pEfterpi Papouli
Cancer Research UK, Clare Hall Laboratories, Blanche Lane, South Mimms, Herts EN6 3LD, United Kingdom
Mol Cell 19:123-33. 2005..Our findings suggest a mechanism by which SUMO and ubiquitin cooperatively control the choice of pathway for the processing of DNA lesions during replication... - Ubiquitylation of yeast proliferating cell nuclear antigen and its implications for translesion DNA synthesisLajos Haracska
Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H 6701 Szeged, Hungary
Proc Natl Acad Sci U S A 103:6477-82. 2006The Rad6-Rad18 ubiquitin-conjugating enzyme complex promotes replication through DNA lesions by means of at least three different pathways: the DNA polymerase (Pol) eta- and zeta-dependent translesion DNA synthesis (TLS) and a Rad5-Mms2-.. - The RING finger ATPase Rad5p of Saccharomyces cerevisiae contributes to DNA double-strand break repair in a ubiquitin-independent mannerShuhua Chen
Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse D 35043, Marburg, Germany
Nucleic Acids Res 33:5878-86. 2005.... - Rad6-Rad18 mediates a eukaryotic SOS response by ubiquitinating the 9-1-1 checkpoint clampYu Fu
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada
Cell 133:601-11. 2008..Here, we demonstrate that the ubiquitination complex Rad6-Rad18 is required for the increased transcription of a large number of yeast genes in response to DNA damage... - Srs2 removes deadly recombination intermediates independently of its interaction with SUMO-modified PCNACyrille Le Breton
CEA DSV Institut de Radiobiologie Cellulaire et Moléculaire, UMR217 CNRS CEA, F 92265 Fontenay aux Roses, France
Nucleic Acids Res 36:4964-74. 2008..In addition, crossover (CO) frequencies are increased in both mutants. The different roles of Srs2, in relation to its eventual recruitment by sumoylated PCNA, are discussed... - Postreplication repair inhibits CAG.CTG repeat expansions in Saccharomyces cerevisiaeDanielle L Daee
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198, USA
Mol Cell Biol 27:102-10. 2007..Like srs2 mutants, expansions are elevated in rad18 and rad5 mutants, as well as the PRR-specific PCNA alleles pol30-K164R and pol30-K127/164R... - Analysis of mitotic and meiotic defects in Saccharomyces cerevisiae SRS2 DNA helicase mutantsF Palladino
Department of Biochemistry and Kaplan Cancer Center, New York University Medical Center, New York 10016
Genetics 132:23-37. 1992..of the SRS2 DNA helicase gene of Saccharomyces cerevisiae has been reported to suppress the UV sensitivity of rad18 mutants. New alleles of SRS2 were recovered using this suppressor phenotype... - Yeast DNA repair proteins Rad6 and Rad18 form a heterodimer that has ubiquitin conjugating, DNA binding, and ATP hydrolytic activitiesV Bailly
Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555 1061, USA
J Biol Chem 272:23360-5. 1997The RAD6 and RAD18 genes of Saccharomyces cerevisiae are required for postreplicative bypass of ultraviolet (UV)-damaged DNA and for UV mutagenesis... - The Saccharomyces cerevisiae RAD30 gene, a homologue of Escherichia coli dinB and umuC, is DNA damage inducible and functions in a novel error-free postreplication repair mechanismJ P McDonald
Section on DNA Replication, Repair and Mutagenesis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2725, USA
Genetics 147:1557-68. 1997..However, unlike rev mutants, no defect in UV-induced reversion was seen in rad30 strains. While rad6 and rad18 are both epistatic to rad30, no epistasis was observed with rev1, rev3, rev7 or rad5, all of which are members of .. - Specific complex formation between yeast RAD6 and RAD18 proteins: a potential mechanism for targeting RAD6 ubiquitin-conjugating activity to DNA damage sitesV Bailly
Department of Biophysics, University of Rochester, New York 14642
Genes Dev 8:811-20. 1994..Here, we show that RAD6 forms a specific complex with the product of the DNA repair gene RAD18. The biological significance of this interaction is attested by the observation that overproduction of the rad6 .. - MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathwayS Broomfield
Department of Microbiology, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK Canada S7N 5E5
Proc Natl Acad Sci U S A 95:5678-83. 1998..The rad6 and rad18 mutants are defective in both pathways, and the rev3 mutant affects only the mutagenesis pathway, but a yeast gene .. - Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repairH D Ulrich
Department of Molecular Cell Biology, Max Planck Institute for Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany
EMBO J 19:3388-97. 2000..We show that two chromatin-associated RING finger proteins, RAD18 and RAD5, play a central role in mediating physical contacts between the members of the RAD6 pathway... - Deletion of the SRS2 gene suppresses elevated recombination and DNA damage sensitivity in rad5 and rad18 mutants of Saccharomyces cerevisiaeA A Friedl
Institute of Radiation Biology, GSF National Research Center for Environment and Health, P O Box 1149, 85758, Oberschleissheim, Germany
Mutat Res 486:137-46. 2001The Saccharomyces cerevisiae genes RAD5, RAD18, and SRS2 are proposed to act in post-replicational repair of DNA damage... - Suppression of a new allele of the yeast RAD52 gene by overexpression of RAD51, mutations in srs2 and ccr4, or mating-type heterozygosityD Schild
Life Sciences Division, Lawrence Berkeley Laboratory, California 94720, USA
Genetics 140:115-27. 1995..rad52-20) is partially suppressed by an srs2 deletion; srs2 mutations normally act to suppress only rad6 and rad18 mutations... - The srs2 suppressor of UV sensitivity acts specifically on the RAD5- and MMS2-dependent branch of the RAD6 pathwayH D Ulrich
Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, D 35043 Marburg, Germany
Nucleic Acids Res 29:3487-94. 2001..Loss-of-function mutations in srs2 suppress the extreme sensitivity towards UV radiation of rad6 and rad18 mutants, both of which are impaired in post-replication DNA repair and damage-induced mutagenesis... - The hyper-gene conversion hpr5-1 mutation of Saccharomyces cerevisiae is an allele of the SRS2/RADH geneL Rong
Department of Biochemistry, New York University Medical Center, New York 10016
Genetics 127:75-85. 1991..This mutation suppresses the UV sensitive phenotype of rad18 mutations in hpr5-1 rad18 double mutants by channeling the aborted repair events into a recombination repair .. - The error-free component of the RAD6/RAD18 DNA damage tolerance pathway of budding yeast employs sister-strand recombinationHengshan Zhang
Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
Proc Natl Acad Sci U S A 102:15954-9. 2005..We have investigated this mechanism in Saccharomyces cerevisiae, in which it is the major component of the RAD6/RAD18 pathway, by transforming an isogenic set of rad1Delta excision-defective strains with plasmids that carry a single .. - The repair of DNA methylation damage in Saccharomyces cerevisiaeW Xiao
Department of Microbiology, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada
Curr Genet 30:461-8. 1996..We found that cells carrying rad6, rad18, rad50 and rad52 single mutations are far more sensitive to killing by MMS than the mag1 mutant, that double .. - Pol32, a subunit of Saccharomyces cerevisiae DNA polymerase delta, suppresses genomic deletions and is involved in the mutagenic bypass pathwayMeng Er Huang
UMR6061 CNRS, Genetique et Developpement, Faculte de Medecine, 35043 Rennes, France
Genetics 160:1409-22. 2002..Taken together, these observations indicate that Pol32 is important in ensuring genome stability and in mutagenesis... - SUMO-modified PCNA recruits Srs2 to prevent recombination during S phaseBoris Pfander
Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Nature 436:428-33. 2005..Our finding suggests a model in which SUMO-modified PCNA recruits Srs2 in S phase in order to prevent unwanted recombination events of replicating chromosomes... - The product of Saccharomyces cerevisiae WHIP/MGS1, a gene related to replication factor C genes, interacts functionally with DNA polymerase deltaD Branzei
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, 980 8578, Japan
Mol Genet Genomics 268:371-86. 2002..Possible roles of Mgs1, DNA polymerase delta, Rad18 and Mms2 in replication and replication fork restart are discussed. - Requirement of Nse1, a subunit of the Smc5-Smc6 complex, for Rad52-dependent postreplication repair of UV-damaged DNA in Saccharomyces cerevisiaeSergio R Santa Maria
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, 301 University Blvd, Galveston, TX 77555 1061, USA
Mol Cell Biol 27:8409-18. 2007In Saccharomyces cerevisiae, postreplication repair (PRR) of UV-damaged DNA occurs by a Rad6-Rad18- and an Mms2-Ubc13-Rad5-dependent pathway or by a Rad52-dependent pathway... - The genome maintenance factor Mgs1 is targeted to sites of replication stress by ubiquitylated PCNAIrene Saugar
Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, EN6 3LD, UK
Nucleic Acids Res 40:245-57. 2012..Our identification of Mgs1 as a UBZ-dependent downstream effector of ubiquitylated PCNA suggests an explanation for the ambivalent role of the protein in damage processing... - Elg1, the major subunit of an alternative RFC complex, interacts with SUMO-processing proteinsOren Parnas
Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel
Cell Cycle 10:2894-903. 2011..Thus our results highlight the many important roles played by Elg1, some of which are PCNA-dependent and some PCNA-independent... - The role of Holliday junction resolvases in the repair of spontaneous and induced DNA damageNeta Agmon
Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 69979, Israel
Nucleic Acids Res 39:7009-19. 2011..Finally, we show that yeast cells are unable to carry out meiosis in the absence of both resolvases. Our results show that both Yen1 and Mms4/Mus81 play important (although not identical) roles during vegetative growth and in meiosis... - Homologous recombination-dependent rescue of deficiency in the structural maintenance of chromosomes (Smc) 5/6 complexAlejandro Chavez
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 286:5119-25. 2011..These data as a whole highlight a role for Smc5/6 and Sgs1 in the resolution of Mph1-dependent HR intermediates... - Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruptionSierra Colavito
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
Nucleic Acids Res 37:6754-64. 2009..These results highlight the importance of Rad51 interaction in the anti-recombinase function of Srs2, and provide evidence that this Srs2 function can be uncoupled from its helicase activity... - Regulation of polymerase exchange between Poleta and Poldelta by monoubiquitination of PCNA and the movement of DNA polymerase holoenzymeZhihao Zhuang
Department of Chemistry, 414 Wartik Laboratory, Pennsylvania State University, University Park, PA 16802, USA
Proc Natl Acad Sci U S A 105:5361-6. 2008..Thus the removal of the ubiquitin moiety from PCNA is likely required for the reverse exchange step after the lesion bypass synthesis by Poleta... - RAD18 transmits DNA damage signalling to elicit homologous recombination repairJun Huang
Department of Therapeutic Radiology, Yale University School of Medicine, P O Box 208040, New Haven, CT 06520, USA
Nat Cell Biol 11:592-603. 2009..Here we describe a new role for the E3 ligase RAD18 as the integral component in translating the damage response signal to orchestrate homologous recombination repair .. - Post-replication repair suppresses duplication-mediated genome instabilityChristopher D Putnam
Ludwig Institute for Cancer Research, University of California San Diego School of Medicine, La Jolla, California, United States of America
PLoS Genet 6:e1000933. 2010..Here, we found that the RAD6- and RAD18-dependent post-replication repair (PRR) and the RAD5-, MMS2-, UBC13-dependent error-free PRR branch acted in .. - Srs2 plays a critical role in reversible G2 arrest upon chronic and low doses of UV irradiation via two distinct homologous recombination-dependent mechanisms in postreplication repair-deficient cellsTakashi Hishida
Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
Mol Cell Biol 30:4840-50. 2010..The first (required to suppress HR during PRR) is regulated by PCNA sumoylation, whereas the second (required for HR-dependent recovery following CLUV exposure) is regulated by CDK1-dependent phosphorylation... - The role of replication bypass pathways in dicentric chromosome formation in budding yeastAndrew L Paek
Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA
Genetics 186:1161-73. 2010..Fourth, by studying genes implicated in suppression of GCRs in other studies, we found that inverted repeat fusion has a profile of genetic regulation distinct from these other major forms of GCR formation... - The Rad1-Rad10 complex promotes the production of gross chromosomal rearrangements from spontaneous DNA damage in Saccharomyces cerevisiaeJi Young Hwang
Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Genetics 169:1927-37. 2005..Results presented here suggest that Rad1-Rad10 functions at different stages of GCR formation and that there is an alternative pathway for the GCR formation that is independent of Rad1-Rad10... - A new Saccharomyces cerevisiae strain with a mutant Smt3-deconjugating Ulp1 protein is affected in DNA replication and requires Srs2 and homologous recombination for its viabilityChristine Soustelle
Commissaritat à l Energy Atomique, UMR 217 CNRS CEA, DSV DRR, Fontenay aux Roses, France
Mol Cell Biol 24:5130-43. 2004..These structures are believed to generate different recombination intermediates. Some of them are fixed by recombination, and others require Srs2 to be reversed and fixed by an alternate pathway... - Delineating the requirements for spontaneous DNA damage resistance pathways in genome maintenance and viability in Saccharomyces cerevisiaeNatalie J Morey
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
Genetics 164:443-55. 2003..In diploids, the presence of PRR alone may confer a lethal mutation load or, alternatively, PRR alone may be insufficient to deal with potentially lethal, replication-blocking lesions... - Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoterAdam Wood
Department of Biochemistry, Saint Louis University School of Medicine, 1402 South Grand Boulevard, St Louis, MO 63104, USA
Mol Cell 11:267-74. 2003..These results suggest that Bre1 is the likely E3 enzyme that directs Rad6 to modify chromatin and ultimately to affect gene expression... - Roles of RAD6 epistasis group members in spontaneous polzeta-dependent translesion synthesis in Saccharomyces cerevisiaeBrenda K Minesinger
Biochemistry, Cell and Developmental Biology Program of the Graduate Division of Biological and Biomedical Sciences, Emory University, Atlanta, GA 30322, USA
Genetics 169:1939-55. 2005..data are consistent with a model in which Polzeta-dependent mutagenesis relies on the presence of either Rad5 or Rad18, which promote two distinct error-prone pathways that partially overlap with respect to lesion specificity... - Previously uncharacterized genes in the UV- and MMS-induced DNA damage response in yeastDenise Hanway
Department of Chemistry and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 99:10605-10. 2002..Epistatsis analysis of four of the genes was performed to determine the DNA damage repair pathways in which the protein products function... - Involvement of the yeast DNA polymerase delta in DNA repair in vivoL Giot
Institut Curie Biologie, Centre Universitaire, Orsay, France
Genetics 146:1239-51. 1997..SDP5 is most probably the p55 subunit of Pol delta of S. cerevisiae and seems to be associated with the catalytic subunit for both DNA replication and DNA repair... - Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage responseBryan M O'Neill
Department of Chemistry, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
Nucleic Acids Res 32:6519-30. 2004..Tof1 is known to be involved in stabilizing stalled replication forks and our data suggest that Wss1 and Psy2 similarly function to stabilize or process stalled or collapsed replication forks... - Requirement of ELC1 for RNA polymerase II polyubiquitylation and degradation in response to DNA damage in Saccharomyces cerevisiaeBalazs Ribar
Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, Galveston, TX 77555 1061, USA
Mol Cell Biol 26:3999-4005. 2006.... - Constitutive fusion of ubiquitin to PCNA provides DNA damage tolerance independent of translesion polymerase activitiesLandon Pastushok
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada
Nucleic Acids Res 38:5047-58. 2010..As expected, the DNA damage resistance provided by PCNA.Ub is not dependent on RAD18 or UBC13. Surprisingly, inactivation of TLS polymerases did not abolish PCNA... - The RAD6/BRE1 histone modification pathway in Saccharomyces confers radiation resistance through a RAD51-dependent process that is independent of RAD18John C Game
Life Sciences Division, Lawrence Berkeley National Laboratory, CA 94720, USA
Genetics 173:1951-68. 2006..We conclude that IR resistance conferred by BRE1 and DOT1 is mediated through homologous recombinational repair, not postreplication repair, and confirm findings of a G1 checkpoint role for the RAD6/BRE1/DOT1 pathway... - Phosphorylation of Rad55 on serines 2, 8, and 14 is required for efficient homologous recombination in the recovery of stalled replication forksKristina Herzberg
Section of Microbiology, University of California, Davis, Davis, CA 95616 8665, USA
Mol Cell Biol 26:8396-409. 2006..These results suggest that Rad55-S2,8,14 phosphorylation activates recombinational repair, allowing for faster recovery after genotoxic stress... - Meiotic recombination and sporulation in repair-deficient strains of yeastE L Dowling
Genetics 109:283-302. 1985..rad1, rad2, rad3, rad4, rad10, rad14 and rad16), and three were defective in mutagenic repair (rad5, rad9 and rad18)... - Rad18/Rad5/Mms2-mediated polyubiquitination of PCNA is implicated in replication completion during replication stressDana Branzei
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, 980 8578, Japan
Genes Cells 9:1031-42. 2004..mutants hys2-1 and cdc2-1 as well as the synthetic lethality of cdc2-1 pol32delta mutants, suggesting a role for Rad18 in modulating DNA replication... - The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 genes are required for tolerating irreparable, ultraviolet-induced DNA damageA G Paulovich
Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Genetics 150:75-93. 1998..Therefore, checkpoint genes not only control cell cycle progression in response to damage, but also play a role in accommodating DNA damage during replication... - Domains required for dimerization of yeast Rad6 ubiquitin-conjugating enzyme and Rad18 DNA binding proteinV Bailly
Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston 77555 1061, USA
Mol Cell Biol 17:4536-43. 1997..Rad6 mediates its DNA repair role via its association with Rad18, whose DNA binding activity may target the Rad6-Rad18 complex to damaged sites in DNA... - Characterization of postreplication repair in Saccharomyces cerevisiae and effects of rad6, rad18, rev3 and rad52 mutationsL Prakash
Mol Gen Genet 184:471-8. 1981..The rad6 mutant does not carry out postreplication repair, the rad18 and rad52 mutants show great inhibition while the rev3 mutation does not affect postreplication repair... - PCNA monoubiquitylation and DNA polymerase eta ubiquitin-binding domain are required to prevent 8-oxoguanine-induced mutagenesis in Saccharomyces cerevisiaePatricia Auffret van der Kemp
CEA, IRCM, UMR217 CNRS Radiobiologie Moléculaire et Cellulaire, 18 route du Panorama, BP6, 92265 Fontenay aux Roses, France
Nucleic Acids Res 37:2549-59. 2009..Here, we present evidence for cooperation between Rad18-Rad6-dependent monoubiquitylation of PCNA at K164, the damage-tolerant DNA polymerase eta and the mismatch repair .. - Methylation of histone H3 lysine-79 by Dot1p plays multiple roles in the response to UV damage in Saccharomyces cerevisiaeLindsey J Bostelman
Department of Biology, Denison University, 213 Talbot Hall, Granville, Ohio 43023, USA
DNA Repair (Amst) 6:383-95. 2007..The overall results indicate the existence of distinct and separable roles of histone H3 lysine-79 methylation in the response to UV damage, potentially serving to coordinate the various repair processes... - DNA sequence selective adenine alkylation, mechanism of adduct repair, and in vivo antitumor activity of the novel achiral seco-amino-cyclopropylbenz[e]indolone analogue of duocarmycin AS-I-145Konstantinos Kiakos
Cancer Research Drug DNA Interactions Research Group, Department of Oncology, University College London, London, United Kingdom
Mol Cancer Ther 6:2708-18. 2007..adduct elimination occurred in a transcription-coupled manner and was dependent on a functional NER pathway and Rad18. The involvement of NER as the predominant excision pathway was confirmed in mammalian DNA repair mutant cells... - UV sensitive mutations in histone H3 in Saccharomyces cerevisiae that alter specific K79 methylation states genetically act through distinct DNA repair pathwaysMargery L Evans
Department of Biology, Denison University, Granville, OH 43023, USA
Curr Genet 53:259-74. 2008.... - Identification of pathways controlling DNA damage induced mutation in Saccharomyces cerevisiaeEwa T Lis
Department of Chemistry, The Scripps Research Institute, CB262R, 10550N Torrey Pines Road, La Jolla, CA 92037, United States
DNA Repair (Amst) 7:801-10. 2008.... - Analysis of the spontaneous mutator phenotype associated with 20S proteasome deficiency in S. cerevisiaeJustyna McIntyre
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Poland
Mutat Res 593:153-63. 2006..occurring spontaneously in yeast cells deficient in 20S proteasome function are connected with the unique Rad6/Rad18-dependent error-prone translesion DNA synthesis (TLS) requiring the activities of both TLS polymerases: Pol eta .. - Multiple pathways cooperate to facilitate DNA replication fork progression through alkylated DNAMaría Victoria Vázquez
Centro de Biologia Molecular Severo Ochoa CSIC UAM, Cantoblanco, Madrid, Spain
DNA Repair (Amst) 7:1693-704. 2008.... - RAD6-RAD18-RAD5-pathway-dependent tolerance to chronic low-dose ultraviolet lightTakashi Hishida
Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
Nature 457:612-5. 2009..Here we examine the response of yeast cells to CLUV and identify a key role for the RAD6-RAD18-RAD5 error-free postreplication repair (RAD6 error-free PRR) pathway in promoting cell growth and survival... - Ubc9- and mms21-mediated sumoylation counteracts recombinogenic events at damaged replication forksDana Branzei
FIRC Institute of Molecular Oncology Foundation and Department of Biomedical Sciences and Biotechnology, Universita degli Studi di Milano, Via Adamello 16, 20139 Milan, Italy
Cell 127:509-22. 2006..Our results indicate that Ubc9- and Mms21-mediated sumoylation functions as a regulatory mechanism, different from that of replication checkpoints, to prevent pathological accumulation of cruciform structures at damaged forks... - Isolation of the RAD18 gene of Saccharomyces cerevisiae and construction of rad18 deletion mutantsF Fabre
Institut Curie Biologie, Centre Universitaire, Orsay, France
Mol Gen Genet 215:425-30. 1989The RAD18 gene of Saccharomyces cerevisiae is involved in mutagenic DNA repair. We describe its isolation from a yeast library introduced into the centromeric YCp50 vector, a low copy number plasmid... - Suppression of genetic defects within the RAD6 pathway by srs2 is specific for error-free post-replication repair but not for damage-induced mutagenesisStacey Broomfield
Department of Microbiology and Immunology, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK S7N 5E5, Canada
Nucleic Acids Res 30:732-9. 2002srs2 was isolated during a screen for mutants that could suppress the UV-sensitive phenotype of rad6 and rad18 cells... - A biological network in Saccharomyces cerevisiae prevents the deleterious effects of endogenous oxidative DNA damageMeng Er Huang
Ludwig Institute for Cancer Research, CMME 3058, 9500 Gilman Drive, La Jolla, California 92093, USA
Mol Cell 17:709-20. 2005..These findings may provide insight in understanding the consequences of various pathophysiological processes in regard to genomic instability... - Cooperation of replication protein A with the ubiquitin ligase Rad18 in DNA damage bypassDiana Huttner
Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts, UK
Cell Cycle 7:3629-33. 2008..Both in yeast and in mammalian cells, RPA physically interacts with Rad18, the ubiquitin ligase responsible for PCNA mono-ubiquitylation... - The RAD24 (= Rs1) gene product of Saccharomyces cerevisiae participates in two different pathways of DNA repairF Eckardt-Schupp
Genetics 115:83-90. 1987..Properties of the mutant are discussed which hint at the control of late steps in the pathways...
Research Grants
- REPAIR PATHWAYS FOR IONIZING RADIATION DAMAGE IN YEASTJohn Game; Fiscal Year: 2002..In a related aim, the order of gene function for RAD5O and RAD54 will be tested using different switching regimes with double mutants containing conditional alleles with differing permissive conditions. ..
- Repair Pathways for Ionizing Radiation Damage YeastJohn Game; Fiscal Year: 2006..Recent and existing mutants will be studied in detail with this system to determine the molecular involvement of each gene in DSB repair, sister chromatid exchange and recombination between chromosomes. ..