rubredoxin

Summary

Gene Symbol: rubredoxin
Description: rubredoxin
Alias: PF1282
Species: Pyrococcus furiosus DSM 3638

Top Publications

  1. Blake P, Park J, Zhou Z, Hare D, Adams M, Summers M. Solution-state structure by NMR of zinc-substituted rubredoxin from the marine hyperthermophilic archaebacterium Pyrococcus furiosus. Protein Sci. 1992;1:1508-21 pubmed
    The three-dimensional solution-state structure is reported for the zinc-substituted form of rubredoxin (Rd) from the marine hyperthermophilic archaebacterium Pyrococcus furiosus, an organism that grows optimally at 100 degrees C...
  2. Strop P, Mayo S. Contribution of surface salt bridges to protein stability. Biochemistry. 2000;39:1251-5 pubmed
    ..Here we present the NMR structure of a hyperthermophilic rubredoxin variant (PFRD-XC4) and the thermodynamic analysis of two surface salt bridges by double mutant cycles...
  3. Day M, Hsu B, Joshua Tor L, Park J, Zhou Z, Adams M, et al. X-ray crystal structures of the oxidized and reduced forms of the rubredoxin from the marine hyperthermophilic archaebacterium Pyrococcus furiosus. Protein Sci. 1992;1:1494-507 pubmed
    The structures of the oxidized and reduced forms of the rubredoxin from the archaebacterium, Pyrococcus furiosus, an organism that grows optimally at 100 degrees C, have been determined by X-ray crystallography to a resolution of 1.8 A...
  4. Blake P, Park J, Bryant F, Aono S, Magnuson J, Eccleston E, et al. Determinants of protein hyperthermostability: purification and amino acid sequence of rubredoxin from the hyperthermophilic archaebacterium Pyrococcus furiosus and secondary structure of the zinc adduct by NMR. Biochemistry. 1991;30:10885-95 pubmed
    The purification, amino acid sequence, and two-dimensional 1H NMR results are reported for the rubredoxin (Rd) from the hyperthermophilic archaebacterium Pyrococcus furiosus, an organism that grows optimally at 100 degrees C...
  5. Jenney F, Verhagen M, Cui X, Adams M. Anaerobic microbes: oxygen detoxification without superoxide dismutase. Science. 1999;286:306-9 pubmed
    ..anaerobe Pyrococcus furiosus uses electrons from reduced nicotinamide adenine dinucleotide phosphate, by way of rubredoxin and an oxidoreductase, to reduce superoxide to hydrogen peroxide, which is then reduced to water by peroxidases...
  6. Kurihara K, Tanaka I, Chatake T, Adams M, Jenney F, Moiseeva N, et al. Neutron crystallographic study on rubredoxin from Pyrococcus furiosus by BIX-3, a single-crystal diffractometer for biomacromolecules. Proc Natl Acad Sci U S A. 2004;101:11215-20 pubmed publisher
    The structure of a partially deuterated rubredoxin from the hyperthermophilic archaeon Pyrococcus furiosus, an organism that grows optimally at 100 degrees C, was determined by using the neutron single-crystal diffractometer dedicated for ..
  7. Chandrayan S, Prakash S, Ahmed S, Guptasarma P. Hyperthermophile protein behavior: partially-structured conformations of Pyrococcus furiosus rubredoxin monomers generated through forced cold-denaturation and refolding. PLoS ONE. 2014;9:e80014 pubmed publisher
    ..Here, we describe the behavior of another hyperthermophile protein, the small, monomeric, 53 residues-long rubredoxin from Pyrococcus furiosus (PfRd), which is one of the most thermostable proteins known to man...
  8. Prakash S, Sundd M, Guptasarma P. The key to the extraordinary thermal stability of P. furiosus holo-rubredoxin: iron binding-guided packing of a core aromatic cluster responsible for high kinetic stability of the native structure. PLoS ONE. 2014;9:e89703 pubmed publisher
    Pyrococcus furiosus rubredoxin (PfRd), a small, monomeric, 53 residues-long, iron-containing, electron-transfer protein of known structure is sometimes referred to as being the most structurally-stable protein known to man...
  9. Tian F, Valafar H, Prestegard J. A dipolar coupling based strategy for simultaneous resonance assignment and structure determination of protein backbones. J Am Chem Soc. 2001;123:11791-6 pubmed
    ..Experimental data were collected in less than one week on a small redox protein, rubredoxin, that was 15N enriched but not enriched above 1% natural abundance in 13C...

More Information

Publications16

  1. Chatake T, Kurihara K, Tanaka I, Tsyba I, Bau R, Jenney F, et al. A neutron crystallographic analysis of a rubredoxin mutant at 1.6 A resolution. Acta Crystallogr D Biol Crystallogr. 2004;60:1364-73 pubmed
    A neutron diffraction study has been carried out at 1.6 A resolution on a mutant rubredoxin from Pyrococcus furiosus using the BIX-3 single-crystal diffractometer at the JRR-3 reactor of the Japan Atomic Energy Research Institute...
  2. Ergenekan C, Tan M, Ichiye T. Effects of environment on the structure of Pyrococcus furiosus rubredoxin: a molecular dynamics study. Proteins. 2005;61:823-8 pubmed
    ..of Pyrococcus furiosus have been performed to elucidate the effects of the environment on the structure of rubredoxin, and proteins in general...
  3. Munshi P, Chung S, Blakeley M, Weiss K, Myles D, Meilleur F. Rapid visualization of hydrogen positions in protein neutron crystallographic structures. Acta Crystallogr D Biol Crystallogr. 2012;68:35-41 pubmed publisher
    ..Comparison of perdeuterated rubredoxin structures refined against neutron data sets collected over hours and up to 5 d shows that rapid neutron data ..
  4. Blake P, Lee B, Summers M, Adams M, Park J, Zhou Z, et al. Quantitative measurement of small through-hydrogen-bond and 'through-space' 1H-113Cd and 1H-199Hg J couplings in metal-substituted rubredoxin from Pyrococcus furiosus. J Biomol NMR. 1992;2:527-33 pubmed
    ..difference spectrum, and is demonstrated for measuring 1H-113Cd and 1H-199Hg J couplings in metal-substituted rubredoxin (M(r) approximately 5.4 kDa) from Pyrococcus furiosus...
  5. Meilleur F, Munshi P, Robertson L, Stoica A, Crow L, Kovalevsky A, et al. The IMAGINE instrument: first neutron protein structure and new capabilities for neutron macromolecular crystallography. Acta Crystallogr D Biol Crystallogr. 2013;69:2157-60 pubmed publisher
    The first high-resolution neutron protein structure of perdeuterated rubredoxin from Pyrococcus furiosus (PfRd) determined using the new IMAGINE macromolecular neutron crystallography instrument at the Oak Ridge National Laboratory is ..
  6. Blake P, Day M, Hsu B, Joshua Tor L, Park J, Hare D, et al. Comparison of the X-ray structure of native rubredoxin from Pyrococcus furiosus with the NMR structure of the zinc-substituted protein. Protein Sci. 1992;1:1522-5 pubmed publisher
    The three-dimensional X-ray structures of the oxidized and reduced forms of rubredoxin from Pyrococcus furiosus, determined at -161 degrees C, and the NMR structure of the zinc-substituted protein, determined in solution at 45 degrees C, ..
  7. Anderson J, Hern ndez G, LeMaster D. A billion-fold range in acidity for the solvent-exposed amides of Pyrococcus furiosus rubredoxin. Biochemistry. 2008;47:6178-88 pubmed publisher
    The exchange rates of the static solvent-accessible amide hydrogens of Pyrococcus furiosus rubredoxin range from near the diffusion-limited rate to a billion-fold slower for the non-hydrogen-bonded Val 38 (eubacterial numbering)...