Gene Symbol: PF_RS04945
Description: DNA polymerase III sliding clamp
Species: Pyrococcus furiosus DSM 3638
Komori K, Ishino Y. Replication protein A in Pyrococcus furiosus is involved in homologous DNA recombination. J Biol Chem. 2001;276:25654-60 pubmed publisher
..furiosus cells. These results indicate that PfuRPA plays important roles in the homologous DNA recombination in P. furiosus...
Fujikane R, Shinagawa H, Ishino Y. The archaeal Hjm helicase has recQ-like functions, and may be involved in repair of stalled replication fork. Genes Cells. 2006;11:99-110 pubmed
..The functional interaction of Hjm with PCNA for its helicase activity further suggests that the Hjm works at stalled replication forks, as a member of the reconstituted replisomes to restart replication. ..
Komori K, Sakae S, Shinagawa H, Morikawa K, Ishino Y. A Holliday junction resolvase from Pyrococcus furiosus: functional similarity to Escherichia coli RuvC provides evidence for conserved mechanism of homologous recombination in Bacteria, Eukarya, and Archaea. Proc Natl Acad Sci U S A. 1999;96:8873-8 pubmed
..These results support the notion that the formation and resolution of Holliday junction is the common mechanism of homologous recombination in the three domains of life. ..
Cann I, Ishino S, Hayashi I, Komori K, Toh H, Morikawa K, et al
. Functional interactions of a homolog of proliferating cell nuclear antigen with DNA polymerases in Archaea. J Bacteriol. 1999;181:6591-9 pubmed
..This is the first stage in elucidating the archaeal DNA replication mechanism...
Matsumiya S, Ishino Y, Morikawa K. Crystal structure of an archaeal DNA sliding clamp: proliferating cell nuclear antigen from Pyrococcus furiosus. Protein Sci. 2001;10:17-23 pubmed publisher
..furiosus DNA polymerases in the absence of the clamp loader replication factor C in vitro...
Cann I, Ishino S, Yuasa M, Daiyasu H, Toh H, Ishino Y. Biochemical analysis of replication factor C from the hyperthermophilic archaeon Pyrococcus furiosus. J Bacteriol. 2001;183:2614-23 pubmed publisher
..furiosus. We propose that PfuRFC is required for efficient loading of PfuPCNA and that the role of RFC in processive DNA synthesis is conserved in Archaea and Eucarya...
Matsumiya S, Ishino S, Ishino Y, Morikawa K. Intermolecular ion pairs maintain the toroidal structure of Pyrococcus furiosus PCNA. Protein Sci. 2003;12:823-31 pubmed publisher
..The comparison of the crystal structures revealed intriguing conformational flexibility of each domain in the PfuPCNA subunit. This structural versatility of PCNA may be involved in the mechanisms for ring opening and closing...
Matsumiya S, Ishino S, Ishino Y, Morikawa K. Physical interaction between proliferating cell nuclear antigen and replication factor C from Pyrococcus furiosus. Genes Cells. 2002;7:911-22 pubmed
..A movement of the C-terminal region of the PCNA monomer by PIP-box binding may cause the PCNA ring to be more rigid, suitable for its functions...