Gene Symbol: PF_RS03035
Description: ornithine carbamoyltransferase
Species: Pyrococcus furiosus DSM 3638
Legrain C, Villeret V, Roovers M, Gigot D, Dideberg O, Pi rard A, et al
. Biochemical characterisation of ornithine carbamoyltransferase from Pyrococcus furiosus. Eur J Biochem. 1997;247:1046-55 pubmed
..Recombinant P. furiosus OTCase synthesised by Escherichia coli proved less stable than the native enzyme. In Saccharomyces cerevisiae, however, an enzyme apparently identical to the native one could be obtained...
Villeret V, Clantin B, Tricot C, Legrain C, Roovers M, Stalon V, et al
. The crystal structure of Pyrococcus furiosus ornithine carbamoyltransferase reveals a key role for oligomerization in enzyme stability at extremely high temperatures. Proc Natl Acad Sci U S A. 1998;95:2801-6 pubmed
..The present crystallographic analysis of PF OTCase provides a structural illustration that oligomerization can play a major role in extreme thermal stabilization...
Massant J, Verstreken P, Durbecq V, Kholti A, Legrain C, Beeckmans S, et al
. Metabolic channeling of carbamoyl phosphate, a thermolabile intermediate: evidence for physical interaction between carbamate kinase-like carbamoyl-phosphate synthetase and ornithine carbamoyltransferase from the hyperthermophile Pyrococcus furiosus. J Biol Chem. 2002;277:18517-22 pubmed
..Therefore, by physically interacting with each other, CKase and OTCase prevent the thermodenaturation of carbamoyl phosphate in the aqueous cytoplasmic environment...
Massant J, Wouters J, Glansdorff N. Refined structure of Pyrococcus furiosus ornithine carbamoyltransferase at 1.87 A. Acta Crystallogr D Biol Crystallogr. 2003;59:2140-9 pubmed
..However, the interfaces between catalytic trimers in the dodecameric oligomer are mainly hydrophobic and also involve aromatic-aromatic and cation-pi interactions...
Roovers M, Hethke C, Legrain C, Thomm M, Glansdorff N. Isolation of the gene encoding Pyrococcus furiosus ornithine carbamoyltransferase and study of its expression profile in vivo and in vitro. Eur J Biochem. 1997;247:1038-45 pubmed
..Upstream from argF is a putative gene for diphthine synthetase, a eukaryotic enzyme assumed to occur also in archaea but not in bacteria. ..
Roovers M, Sanchez R, Legrain C, Glansdorff N. Experimental evolution of enzyme temperature activity profile: selection in vivo and characterization of low-temperature-adapted mutants of Pyrococcus furiosus ornithine carbamoyltransferase. J Bacteriol. 2001;183:1101-5 pubmed
..The mutants were double ones, still complementing at 15 degrees C, a temperature already in the psychrophilic range. Their kinetic analysis is reported. ..
Clantin B, Tricot C, Lonhienne T, Stalon V, Villeret V. Probing the role of oligomerization in the high thermal stability of Pyrococcus furiosus ornithine carbamoyltransferase by site-specific mutants. Eur J Biochem. 2001;268:3937-42 pubmed
..Finally, the E25Q/M29A/W33A variant shows a very large decrease in heat stability and is a trimer. These results suggest that extreme thermal stabilization of this OTCase is achieved in part through oligomerization...