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Genomes and Genes | puhASummaryGene Symbol: puhA Description: reaction centre H protein Species: Top Publications
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Publications
Structure of the reaction center from Rhodobacter sphaeroides R-26 and 2.4.1: symmetry relations and sequence comparisons between different speciesH Komiya
University of California, Los Angeles 90024
Proc Natl Acad Sci U S A 85:9012-6. 1988..A method is presented for characterizing the exposure of alpha-helices to the membrane based on the periodicity of conserved residues. This method may prove useful for modeling the three-dimensional structures of membrane proteins...- Structure of the reaction center from Rhodobacter sphaeroides R-26: protein-cofactor (quinones and Fe2+) interactionsJ P Allen
University of California, San Diego, La Jolla 92093
Proc Natl Acad Sci U S A 85:8487-91. 1988..The interactions between QB and the residues that have been changed in herbicide-resistant mutants are described. The environment of the nonheme iron is compared to the environments of metal ions in other proteins... - A new metal-binding site in photosynthetic bacterial reaction centers that modulates QA to QB electron transferL M Utschig
Chemistry Division, Argonne National Laboratory, Illinois 60439, USA
Biochemistry 37:8278-81. 1998..Inspection of the RC crystal structure suggests a cluster of histidine ligands located beneath the QB binding pocket as a potential binding site... - Quinone (QB) reduction by B-branch electron transfer in mutant bacterial reaction centers from Rhodobacter sphaeroides: quantum efficiency and X-ray structureM L Paddock
Department of Physics, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
Biochemistry 44:6920-8. 2005..In this structure a nonfunctional quinone was found to occupy a new site near M258 and M268. The implications of this work to trap intermediate states are discussed... - The structure of a mutant photosynthetic reaction center shows unexpected changes in main chain orientations and quinone positionP R Pokkuluri
Biosciences Division, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439, USA
Biochemistry 41:5998-6007. 2002.... - Identification of the proton pathway in bacterial reaction centers: inhibition of proton transfer by binding of Zn2+ or Cd2+M L Paddock
Department of Physics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 96:6183-8. 1999..The large change in the rate of proton transfer caused by the stoichiometric binding of the metal ion shows that there is one dominant site of proton entry into the RC from which proton transfer to QB-* occurs... - Structure of the photosynthetic reaction centre from Rhodobacter sphaeroides at 2.65 A resolution: cofactors and protein-cofactor interactionsU Ermler
Max Planck Institut fur Biophysik, Frankfurt Main, Germany
Structure 2:925-36. 1994..There are discrepancies between these structures, however, and to resolve these we have determined the structure to higher resolution than before, using a new crystal form... - Structural details of an interaction between cardiolipin and an integral membrane proteinK E McAuley
Division of Biochemistry, University of Glasgow, Glasgow, G12 8QQ, United Kingdom
Proc Natl Acad Sci U S A 96:14706-11. 1999..The possible significance of this interaction between an integral membrane protein and cardiolipin is considered... - Interruption of the water chain in the reaction center from Rhodobacter sphaeroides reduces the rates of the proton uptake and of the second electron transfer to QBL Baciou
Max Planck Institut fur Biophysik, Frankfurt Main, Germany
Biochemistry 34:7967-72. 1995..The relative decrease of the kAB(2) rate values in the mutants is more pronounced above pH 8. Our results indicate that the mutations have specifically altered the pathway of proton transfer to QB.(ABSTRACT TRUNCATED AT 250 WORDS).. - Preliminary characterization by X-ray diffraction of crystals of photochemical reaction centres from wild-type Rhodopseudomonas spheroidesA Ducruix
J Mol Biol 193:419-21. 1987..Orthorhombic crystals (space group P2(1)2(1)2(1)) diffract to 3.5 A resolution. The unit cell parameters are a = 142.5 A, b = 141.5 A, c = 80 A; they are compatible with the presence of one reaction centre per asymmetric unit... - The bacterial photosynthetic reaction center as a model for membrane proteinsD C Rees
Department of Chemistry and Biochemistry, University of California, Los Angeles 90024
Annu Rev Biochem 58:607-33. 1989 - Comparison of reaction centers from Rhodobacter sphaeroides and Rhodopseudomonas viridis: overall architecture and protein-pigment interactionsO El-Kabbani
Biological and Medical Research Division, Argonne National Laboratory, Illinois 60439
Biochemistry 30:5361-9. 1991..sphaeroides RC structure previously described by Yeates et al. [(1988) Proc. Natl. Acad. Sci. U.S.A. 85, 7993-7997] and Allen et al. [(1988) Proc. Natl. Acad. Sci. U.S.A. 85, 8487-8491]... - Specific alteration of the oxidation potential of the electron donor in reaction centers from Rhodobacter sphaeroidesX Lin
Department of Chemistry and Biochemistry, Arizona State University, Tempe 85287 1604
Proc Natl Acad Sci U S A 91:10265-9. 1994..These results suggest a role for protein-cofactor interactions--in particular, histidine-donor interactions--in establishing the redox potentials needed for electron transfer in biological systems... - X-ray structure determination of the cytochrome c2: reaction center electron transfer complex from Rhodobacter sphaeroidesHerbert L Axelrod
Department of Physics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0319, USA
J Mol Biol 319:501-15. 2002..This domain, in addition to contributing to the binding, may help steer the unbound proteins toward the right conformation... - Design of a redox-linked active metal site: manganese bound to bacterial reaction centers at a site resembling that of photosystem IIM Thielges
Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA
Biochemistry 44:7389-94. 2005..These reaction centers illustrate the successful design of a redox active metal center in a protein complex... - Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides at 2.35A resolutionGergely Katona
Department of Molecular Biotechnology, Chalmers University of Technology, P O Box 462, SE 405 30 Gothenburg, Sweden
J Mol Biol 331:681-92. 2003..Comparisons with the X-ray structures of this reaction centre deriving from detergent-based crystals are drawn, indicating that a slight compression occurs in this lipid-rich environment... - X-Ray structure determination of three mutants of the bacterial photosynthetic reaction centers from Rb. sphaeroides; altered proton transfer pathwaysQiang Xu
Department of Physics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
Structure 12:703-15. 2004..The electrostatic changes near Glu-H173 cause it to be a good proton donor and acceptor, and the structural changes create a cavity which accommodates water molecules that connect Glu-H173 to other proton transfer components... - Construction and characterization of a mutant of Rhodobacter sphaeroides with the reaction center as the sole pigment-protein complexM R Jones
Department of Molecular Biology and Biotechnology, University of Sheffield, U K
Biochemistry 31:4458-65. 1992..A second strain, named RCLH11, which is devoid of the peripheral LH2 antenna complex has also been constructed. A description of the properties of these strains is presented... - Crystallization of reaction center from Rhodopseudomonas sphaeroides: preliminary characterizationJ P Allen
Proc Natl Acad Sci U S A 81:4795-9. 1984..The two bands at 535 and 544 nm assigned to the Qx transitions of the bacteriopheophytins were resolved and preliminary orientations of some of the pigments were obtained... - Structure of the membrane-bound protein photosynthetic reaction center from Rhodobacter sphaeroidesC H Chang
Biological and Medical Research Division, Argonne National Laboratory, Illinois 60439
Biochemistry 30:5352-60. 1991..The conserved residues at the N-termini of the L and M chains were identified as recognition sites for the H chain... - Protein regulation of carotenoid binding; gatekeeper and locking amino acid residues in reaction centers of Rhodobacter sphaeroidesAleksander W Roszak
Department of Chemistry, IBLS, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
Structure 12:765-73. 2004..The study reveals the specific molecular interactions that control how the carotenoid protects the photosynthetic apparatus against photo-induced oxidative destruction... - Structural consequences of the replacement of glycine M203 with aspartic acid in the reaction center from Rhodobacter sphaeroidesP K Fyfe
Krebs Institute for Biomolecular Research and Robert Hill Institute for Photosynthesis, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2UH, United Kingdom
Biochemistry 39:5953-60. 2000..We examine whether the observed structural changes can provide new or alternative explanations for the absorbance and electron-transfer properties of reaction centers with the FM197R and GM203D mutations... - Structural homology of reaction centers from Rhodopseudomonas sphaeroides and Rhodopseudomonas viridis as determined by x-ray diffractionJ P Allen
Proc Natl Acad Sci U S A 83:8589-93. 1986..Unlike the RCs from R. viridis, both quinones are retained in the RCs from R. sphaeroides. The secondary quinone is located near the position related by the 2-fold symmetry axis to the primary quinone... - Individual interactions influence the crystalline order for membrane proteinsA Camara-Artigas
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 1604, USA
Acta Crystallogr D Biol Crystallogr 57:1281-6. 2001..The results suggest that the contact regions are crucial for obtaining highly ordered crystals of membrane proteins... - Light-induced structural changes in photosynthetic reaction center: implications for mechanism of electron-proton transferM H Stowell
Division of Chemistry and Chemical Engineering, 147 75CH, California Institute of Technology, Pasadena, CA 91125, USA
Science 276:812-6. 1997..In addition, several water channels (putative proton pathways) leading from the QB pocket to the surface of the RC were delineated, one of which leads directly to the membrane surface... - Determination of the binding sites of the proton transfer inhibitors Cd2+ and Zn2+ in bacterial reaction centersH L Axelrod
Department of Physics 0319, 9500 Gilman Drive, University of California at San Diego, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 97:1542-7. 2000..The position of the Cd(2+) and Zn(2+) localizes the proton entry into the RC near Asp-H124, His-H126, and His-H128. Based on the location of the metal, likely pathways of proton transfer from the aqueous surface to Q(B) are proposed... - X-ray structure analyses of photosynthetic reaction center variants from Rhodobacter sphaeroides: structural changes induced by point mutations at position L209 modulate electron and proton transferA Kuglstatter
Max Planck Institut fur Biophysik, H Hoffmann Strasse 7, 60528 Frankfurt M, Germany
Biochemistry 40:4253-60. 2001..In the Pro L209 --> Glu reaction center, the carboxylic side chain of Glu L209 is located within the water chain, and the binding site of Q(B) remains unchanged compared to the wild-type structure... - Primary structure of the reaction center from Rhodopseudomonas sphaeroidesJ C Williams
Department of Biology, University of California, San Diego, La Jolla 92093
Proteins 1:312-25. 1986.... - Effects of hydrogen bonding to a bacteriochlorophyll-bacteriopheophytin dimer in reaction centers from Rhodobacter sphaeroidesJ P Allen
Department of Chemistry and Biochemistry, Arizona State University, Tempe 85287 1604, USA
Biochemistry 35:6612-9. 1996..The results show that the properties of the heterodimer are different depending on which side is hydrogen-bonded and suggest that the hydrogen bonds alter the energy of the internal charge transfer state in a well-defined manner... - Interactions between lipids and bacterial reaction centers determined by protein crystallographyA Camara-Artigas
Department of Chemistry and Biochemistry and Center for the Study of Early Events in Photosynthesis, Arizona State University, Tempe, AZ 85287 1604, USA
Proc Natl Acad Sci U S A 99:11055-60. 2002.... - Structure of the reaction center from Rhodobacter sphaeroides R-26 and 2.4.1: protein-cofactor (bacteriochlorophyll, bacteriopheophytin, and carotenoid) interactionsT O Yeates
University of California, Los Angeles 90024
Proc Natl Acad Sci U S A 85:7993-7. 1988..sphaeroides 2.4.1 consists largely of aromatic residues of the M subunit. A role of BB in the triplet energy transfer from D to C and the reason for the preferential ease of removal of BB from the RC is proposed... - Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides: monitoring the optical properties of the complex from bacterial cell to crystalK E McAuley-Hecht
Division of Biochemistry and Molecular Biology and Department of Chemistry, University of Glasgow, Glasgow G12 8QQ, United Kingdom
Biochemistry 37:4740-50. 1998..Changes in the optical spectrum of the FM197R/YM177F reaction center are discussed with respect to the altered structure of the complex... - Structure of the reaction center from Rhodobacter sphaeroides R-26: the cofactorsJ P Allen
Proc Natl Acad Sci U S A 84:5730-4. 1987..The structure is well suited to produce light-induced charge separation across the membrane. Most of the structural features predicted from physical and biochemical measurements are confirmed by the x-ray structure... - Structure of the reaction center from Rhodobacter sphaeroides R-26: membrane-protein interactionsT O Yeates
Proc Natl Acad Sci U S A 84:6438-42. 1987..The electrostatic potential of the cofactors was calculated. The results show an asymmetry in the potential between the two possible pathways of electron transfer, with the A branch being preferred electrostatically... - Structure of the reaction center from Rhodobacter sphaeroides R-26: the protein subunitsJ P Allen
Proc Natl Acad Sci U S A 84:6162-6. 1987..The structural homology with RCs from other purple bacteria is discussed. A structure of the complex formed between the water soluble cytochrome c2 and the RC from Rb. sphaeroides is proposed... - An examination of how structural changes can affect the rate of electron transfer in a mutated bacterial photoreaction centreJ P Ridge
Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2UH, U K
Biochem J 351:567-78. 2000.... - Strong effects of an individual water molecule on the rate of light-driven charge separation in the Rhodobacter sphaeroides reaction centerJane A Potter
Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, United Kingdom
J Biol Chem 280:27155-64. 2005..Possible functions of this water molecule are discussed, including a switching role in which the redox potential of the B(A) acceptor is rapidly modulated in response to oxidation of the primary electron donor... - Crystallographic analyses of site-directed mutants of the photosynthetic reaction center from Rhodobacter sphaeroidesA J Chirino
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125
Biochemistry 33:4584-93. 1994..The M219HC diffraction data indicate that the overall tertiary structure of the reaction center is maintained in the absence of the non-heme iron...