Gene Symbol: hemB
Description: delta-aminolevulinic acid dehydratase
Species: Pseudomonas aeruginosa PAO1
Frère F, Reents H, Schubert W, Heinz D, Jahn D. Tracking the evolution of porphobilinogen synthase metal dependence in vitro. J Mol Biol. 2005;345:1059-70 pubmed
Frankenberg N, Kittel T, Hungerer C, Romling U, Jahn D. Cloning, mapping and functional characterization of the hemB gene of Pseudomonas aeruginosa, which encodes a magnesium-dependent 5-aminolevulinic acid dehydratase. Mol Gen Genet. 1998;257:485-9 pubmed
..In Escherichia coli, the enzyme is encoded by the gene hemB. The hemB gene was cloned from Pseudomonas aeruginosa by functional complementation of an E. coli hemB mutant...
Frankenberg N, Erskine P, Cooper J, Shoolingin Jordan P, Jahn D, Heinz D. High resolution crystal structure of a Mg2+-dependent porphobilinogen synthase. J Mol Biol. 1999;289:591-602 pubmed
..We conclude that the observed differences in the active sites of both monomers might be induced by Mg2+-binding to this remote site and propose a structure-based mechanism for this allosteric Mg2+in rate enhancement. ..
Frère F, Schubert W, Stauffer F, Frankenberg N, Neier R, Jahn D, et al
. Structure of porphobilinogen synthase from Pseudomonas aeruginosa in complex with 5-fluorolevulinic acid suggests a double Schiff base mechanism. J Mol Biol. 2002;320:237-47 pubmed
..Based on these structural data, we postulate a catalytic mechanism for P. aeruginosa PBGS initiated by a C-C bond formation between A and P-side ALA, followed by the formation of the intersubstrate Schiff base yielding the product PBG. ..