Gene Symbol: PKC1
Alias: CLY15, HPO2, STT1
- A family of genes required for maintenance of cell wall integrity and for the stress response in Saccharomyces cerevisiaeJ Verna
Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA 93106, USA
Proc Natl Acad Sci U S A 94:13804-9. 1997The PKC1-MPK1 pathway in yeast functions in the maintenance of cell wall integrity and in the stress response. We have identified a family of genes that are putative regulators of this pathway...
- SBF cell cycle regulator as a target of the yeast PKC-MAP kinase pathwayK Madden
Department of Biology, Yale University, Post Office Box 208103, New Haven, CT 06520 8103, USA
Science 275:1781-4. 1997..In the budding yeast, PKC1 functions to activate the SLT2(MPK1) mitogen-activated protein (MAP) kinase cascade, which is required for the ..
- A role for the Pkc1 MAP kinase pathway of Saccharomyces cerevisiae in bud emergence and identification of a putative upstream regulatorJ V Gray
Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0448, USA
EMBO J 16:4924-37. 1997The protein kinase C of Saccharomyces cerevisiae, Pkc1, regulates a MAP kinase, Mpk1, whose activity is stimulated at the G1-S transition of the cell cycle and by perturbations to the cell surface, e.g. induced by heat shock...
- Saccharomyces cerevisiae mid2p is a potential cell wall stress sensor and upstream activator of the PKC1-MPK1 cell integrity pathwayT Ketela
Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1
J Bacteriol 181:3330-40. 1999..mid2Delta mutants can be suppressed by overexpression of upstream elements of the cell integrity pathway, including PKC1, RHO1, WSC1, and WSC2...
- Activation of yeast protein kinase C by Rho1 GTPaseY Kamada
Department of Biochemisty, Johns Hopkins University School of Public Health, Baltimore, Maryland 21205, USA
J Biol Chem 271:9193-6. 1996..defect that is similar to that of mutants in the cell integrity signaling pathway mediated by protein kinase C (Pkc1), which is suppressed by overexpression of Pkc1...
- Molecular analysis reveals localization of Saccharomyces cerevisiae protein kinase C to sites of polarized growth and Pkc1p targeting to the nucleus and mitotic spindleValerie Denis
Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
Eukaryot Cell 4:36-45. 2005..This novel nuclear and spindle localization of Pkc1p may provide a molecular explanation for previous observations that suggest a role for Pkc1p in regulating microtubule function...
- Dynamic, Rho1p-dependent localization of Pkc1p to sites of polarized growthP D Andrews
Department of Biochemistry, MSI Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, UK
J Cell Sci 113:2685-93. 2000..These results suggest that the roles of Pkc1p in both polarized growth and the response to cell wall stress are mediated by dynamic changes in its localization, and suggest an additional potential role in cytokinesis...
- Dissecting the protein kinase C/MAP kinase signalling pathway of Saccharomyces cerevisiaeD E Levin
Department of Biochemistry, Johns Hopkins University, School of Public Health, Baltimore, MD 21205
Cell Mol Biol Res 40:229-39. 1994The PKC1 gene of the budding yeast Saccharomyces cerevisiae encodes a homolog of the alpha, beta, and gamma isoforms of mammalian PKC that is essential for cell growth...
- A downstream target of RHO1 small GTP-binding protein is PKC1, a homolog of protein kinase C, which leads to activation of the MAP kinase cascade in Saccharomyces cerevisiaeH Nonaka
Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita, Japan
EMBO J 14:5931-8. 1995..Molecular cloning of the suppressor gene revealed that the mutation occurred at the pseuodosubstrate site of PKC1, a yeast homolog of mammalian protein kinase C...
- MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase CK Irie
Department of Molecular Biology, Faculty of Science, Nagoya University, Japan
Mol Cell Biol 13:3076-83. 1993The PKC1 gene of Saccharomyces cerevisiae encodes a homolog of mammalian protein kinase C that is required for normal growth and division of yeast cells...
- PDK1 homologs activate the Pkc1-mitogen-activated protein kinase pathway in yeastM Inagaki
Department of Molecular Biology, Graduate School of Science, Nagoya University, and CREST, Japan Science and Technology Corporation, Chikusa ku, Nagoya 464 8602, Japan
Mol Cell Biol 19:8344-52. 1999..A temperature-sensitive mutant, pkh1(D398G) pkh2, was phenotypically similar to mutants defective in the Pkc1-mitogen-activated protein kinase (MAPK) pathway...
- Saccharomyces cerevisiae PKC1 encodes a protein kinase C (PKC) homolog with a substrate specificity similar to that of mammalian PKCM Watanabe
Department of Biochemistry, Johns Hopkins University, School of Public Health, Baltimore, Maryland 21205
J Biol Chem 269:16829-36. 1994The PKC1 gene of the budding yeast Saccharomyces cerevisiae encodes a homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) that is essential for cell growth...
- The Rho1 effector Pkc1, but not Bni1, mediates signalling from Tor2 to the actin cytoskeletonS B Helliwell
Department of Biochemistry, University of Basel, Switzerland
Curr Biol 8:1211-4. 1998..Four Rho1 effectors are known, protein kinase C 1 (Pkc1), the formin-family protein Bni1, the glucan synthase Fks and the signalling protein Skn7 [2,3]...
- Rho1p-Bni1p-Spa2p interactions: implication in localization of Bni1p at the bud site and regulation of the actin cytoskeleton in Saccharomyces cerevisiaeT Fujiwara
Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565 0871, Japan
Mol Biol Cell 9:1221-33. 1998..These results indicate that both Rho1p and Spa2p are involved in the localization of Bni1p at the growth sites where Rho1p regulates reorganization of the actin cytoskeleton through Bni1p...
- Dominant mutations in a gene encoding a putative protein kinase (BCK1) bypass the requirement for a Saccharomyces cerevisiae protein kinase C homologK S Lee
Department of Biochemistry, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205
Mol Cell Biol 12:172-82. 1992The PKC1 gene of Saccharomyces cerevisiae encodes a homolog of mammalian protein kinase C that is required for yeast cell growth and division...
- Phosphorylation of Saccharomyces cerevisiae CTP synthetase at Ser424 by protein kinases A and C regulates phosphatidylcholine synthesis by the CDP-choline pathwayMal Gi Choi
Department of Food Science, Cook College, New Jersey Agricultural Experiment Station, Rutgers University, New Brunswick, New Jersey 08901, USA
J Biol Chem 278:23610-6. 2003....
- Activation of the protein kinase C1 pathway upon continuous heat stress in Saccharomyces cerevisiae is triggered by an intracellular increase in osmolarity due to trehalose accumulationFemke I C Mensonides
Swammerdam Institute of Life Sciences, Department of Molecular Microbial Physiology, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
Appl Environ Microbiol 71:4531-8. 2005..A detailed analysis was carried out of the accumulation of trehalose and the activation of the protein kinase C1 (PKC1) (cell integrity) pathway in both glucose- and ethanol-grown cells upon temperature upshifts within this narrow ..
- KNR4 is a member of the PKC1 signalling pathway and genetically interacts with BCK2, a gene involved in cell cycle progression in Saccharomyces cerevisiaeHelene Martin-Yken
Centre de Bioingenierie Gilbert Durand, UMR CNRS 5504, UR INRA 792, 31077 Toulouse, France
Curr Genet 41:323-32. 2002In budding yeast, PKC1 plays an essential role in cell wall integrity and cell proliferation through a bifurcated PKC1/mitogen-activated protein (MAP) kinase pathway...
- Phosphorylation of the yeast choline kinase by protein kinase C. Identification of Ser25 and Ser30 as major sites of phosphorylationMal Gi Choi
Department of Food Science, Cook College, New Jersey Agricultural Experiment Station, Rutgers University, New Brunswick, New Jersey 08901, USA
J Biol Chem 280:26105-12. 2005..Comparison of phosphopeptide maps of the wild type and S30A mutant choline kinase enzymes phosphorylated by protein kinase C confirmed that Ser30 was also a target site for protein kinase C...
- Pkc1p modifies CPY* degradation in the ERAD pathwayMihai Nita-Lazar
Department of Biochemistry and Cell Biology and the Institute of Cell and Developmental Biology, SUNY Stony Brook, NY, USA
Biochem Biophys Res Commun 332:357-61. 2005..Pkc1p has been identified as a component of the ERAD pathway, because deletion of the pkc1 gene impairs ERAD and causes accumulation of CPY* in the lumen of the ER, most probably because of the ..
- Saccharomyces cerevisiaeTSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complexHsiang Ling Ho
Institute of Biochemistry, School of Life Sciences and Department of Biochemistry, School of Medicine, National Yang Ming University, 155, Sec 2, Li Nong St, Shih Pai, Taipei, 112, Taiwan
Curr Genet 47:273-88. 2005..AVO1 suppressed one class of mutants, whereas active PKC1, AVO2, and SLM1 partially rescued another...
- Relationship between protein kinase C and derepression of different enzymesA P C Salgado
Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro, 35 400 000, MG, Ouro Preto, Brazil
FEBS Lett 532:324-32. 2002The PKC1 gene in the yeast Saccharomyces cerevisiae encodes for protein kinase C which is known to control a MAP kinase cascade consisting of different kinases: Bck1, Mkk1 and Mkk2, and Mpk1...
- Aberrant processing of the WSC family and Mid2p cell surface sensors results in cell death of Saccharomyces cerevisiae O-mannosylation mutantsMark Lommel
Institute of Cell Biology and Plant Physiology, University of Regensburg, 93040 Regensburg Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
Mol Cell Biol 24:46-57. 2004..These phenotypes are partially suppressed by overexpression of upstream elements of the protein kinase C (PKC1) cell integrity pathway, suggesting that the PKC1 pathway is defective in pmt2 pmt4Delta mutants...
- Formin-dependent actin assembly is regulated by distinct modes of Rho signaling in yeastYuqing Dong
Department of Molecular Biology and Genetics, Cornell University, 353 Biotechnology Building, Ithaca, NY 14853, USA
J Cell Biol 161:1081-92. 2003..These results show that formin function is under the control of three distinct, essential Rho signaling pathways...
- Disruption of the Saccharomyces cerevisiae cell-wall pathway gene SLG1 causes hypersensitivity to the antitumor drug bleomycinA Leduc
Centre de Recherche Guy Bernier, Maisonneuve Rosemont Hospital, 5415 de l Assomption, Montreal, Quebec H1T 2M4, Canada
Mol Genet Genomics 269:78-89. 2003..A plasmid that expresses the native SLG1 gene or that increases PKC1 gene dosage restored bleomycin resistance to the slg1Delta mutant...
- A genome-wide overexpression screen in yeast for small-molecule target identificationHendrik Luesch
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Chem Biol 12:55-63. 2005..1), an inhibitor identified from a kinase-directed library, was associated with the overexpression of Pkc1 and a subset of downstream kinases...
- Functional connection between the Clb5 cyclin, the protein kinase C pathway and the Swi4 transcription factor in Saccharomyces cerevisiaeEthel Queralt
Departament de Bioquimica i Biologia Molecular, Universitat de Valencia, C Dr Moliner 50, E 46100 Burjassot, Valencia, Spain
Genetics 171:1485-98. 2005..Interestingly, genes of the PKC pathway rescue the growth defect of clb5 swi4, and pkc1 and slt2 mutations are synthetic lethal with clb5, pointing to a connection between Clb5, the PKC pathway, and Swi4...
- Two proteins from Saccharomyces cerevisiae: Pfy1 and Pkc1, play a dual role in activating actin polymerization and in increasing cell viability in the adaptive response to oxidative stressNuria Pujol
Department de Ciències Mèdiques Bàsiques IRBLleida, Faculty of Medicine, University of Lleida, Lleida Spain
FEMS Yeast Res 9:1196-207. 2009In this work, we show that the proteins Pkc1 and Pfy1 play a role in the repolarization of the actin cytoskeleton and in cell survival in response to oxidative stress...
- New mutant versions of yeast FACT subunit Spt16 affect cell integrityAllyson F O'Donnell
Department of Biochemistry and Molecular Biology, Dalhousie University, Sir Charles Tupper Medical Building, 5850 College Street, Halifax, NS, B3H 1X5, Canada
Mol Genet Genomics 282:487-502. 2009..The overexpression of components of protein kinase C (Pkc1) signaling diminishes this spt16 ssd1-d temperature sensitivity, whereas gene deletions eliminating components of ..
- Mechanisms for concentrating Rho1 during cytokinesisSatoshi Yoshida
Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Division of Hematology Oncology, Children s Hospital Boston, Boston, Massachusetts 02115, USA
Genes Dev 23:810-23. 2009..These findings comprehensively define the targeting mechanisms of Rho1 essential for cytokinesis in yeast, and are likely to be relevant to cytokinesis in other organisms...
- Cell wall integrity MAPK pathway is essential for lipid homeostasisLilia R Nunez
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
J Biol Chem 283:34204-17. 2008..These results indicate that an intact cell wall integrity pathway is required for maintaining proper lipid homeostasis in yeast, especially when cells are grown in the absence of inositol...
- Involvement of Saccharomyces cerevisiae Avo3p/Tsc11p in maintaining TOR complex 2 integrity and coupling to downstream signalingHsiang Ling Ho
Institute of Biochemistry and Molecular Biology, School of Life Sciences, National Yang Ming University, 155, Sec 2, Li Nong St, Taipei 11221, Taiwan
Eukaryot Cell 7:1328-43. 2008..Our results also suggest that Avo2p/Slm1p-mediated signaling, but not Avo1p-mediated signaling, links to Rho1p activation specifically through the Rho1p-guanine nucleotide exchange factor Tus1p...
- G1/S cyclin-dependent kinase regulates small GTPase Rho1p through phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiaeKeiko Kono
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277 8562, Japan
Mol Biol Cell 19:1763-71. 2008..We propose that Cln2p/Cdc28p-dependent phosphorylation of Tus1p is required for appropriate temporal and spatial activation of Rho1p at the G1/S transition...
- Histone H2B ubiquitylation and H3 lysine 4 methylation prevent ectopic silencing of euchromatic loci important for the cellular response to heatAmy Leung
Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
Mol Biol Cell 22:2741-53. 2011..Our data reveal that H2B ubiquitylation/H3K4 methylation play an important role in limiting ectopic association of silencing proteins with euchromatic genes important for cell wall integrity and the response to heat...
- Rot1 plays an antagonistic role to Clb2 in actin cytoskeleton dynamics throughout the cell cycleM Angeles Juanes
Departament de Bioquimica i Biologia Molecular, Facultat de Ciencies Biologiques, Universitat de Valencia, 46100 Burjassot Valencia, Spain
J Cell Sci 120:2390-401. 2007..Several genetic interactions suggest a link between Rot1 and the ubiquitin-proteasome system and we show that the Clb2 cyclin is not properly degraded in rot1 cells...
- Thermosensitivity of the Saccharomyces cerevisiae gpp1gpp2 double deletion strain can be reduced by overexpression of genes involved in cell wall maintenanceIwona Wojda
Department of Invertebrate Immunology, Institute of Biology, Maria Curie Sklodowska University, Akademicka 19, 20 033, Lublin, Poland
Arch Microbiol 188:175-84. 2007..We also confirmed that the internal glycerol level changed in cells exposed to cell wall perturbation...
- The identification and characterisation of a functional interaction between arginyl-tRNA-protein transferase and topoisomerase IICatherine R Barker
School of Clinical Sciences, Division of Gastroenterology, The University of Liverpool, Henry Wellcome Laboratory of Molecular and Cellular Gastroenterology, Crown Street, Liverpool L69 3BX, UK
Biochem Biophys Res Commun 342:596-604. 2006..Ate1 is a component of the N-end rule protein degradation pathway which targets proteins for degradation. We also propose a previously unidentified role for Ate1 in modulating the level of topoisomerase II through the cell cycle...
- The identification of a functional interaction between PKC and topoisomerase IINathalie A P Mouchel
Compton Paddock Laboratories, Frilsham Home Farm Business Unit, Yattendon, Thatcham RG 18 0XT, UK
FEBS Lett 580:51-7. 2006..We identified an interaction between the catalytic domain of the yeast protein kinase 1 enzyme (Pkc1) and the N-terminal domain of the S. cerevisiae topoisomerase II. The S...
- The 'interactome' of the Knr4/Smi1, a protein implicated in coordinating cell wall synthesis with bud emergence in Saccharomyces cerevisiaeFadi Basmaji
Laboratoire de Biotechnologie et Bioprocédés, UMR CNRS 5504 and INRA 792, 135, Avenue de Rangueil, 31077 Toulouse Cedex 04, France
Mol Genet Genomics 275:217-30. 2006The integrity of the Saccharomyces cerevisiae cell wall requires a functional Pkc1-Slt2 MAP kinase pathway that contributes to transient growth arrest, enabling coordination of cell division with cell wall remodelling...
- Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutantCleslei F Zanelli
Department of Biological Sciences, School of Pharmaceutical Sciences, Sao Paulo State University, UNESP, Rodovia Araraquara Jaú, km 01, Araraquara, São Paulo 14801 902, Brazil
Genetics 171:1571-81. 2005..We have previously shown that high-copy PKC1 suppresses the phenotype of tif51A-1, a temperature-sensitive mutant of eIF5A in S. cerevisiae...
- Zds2p regulates Swe1p-dependent polarized cell growth in Saccharomyces cerevisiae via a novel Cdc55p interaction domainKimberly Yasutis
Departments of Biology and Cell Biology, University of Virginia, Charlottesville, VA 22904, USA
Mol Biol Cell 21:4373-86. 2010..This negative regulation requires the CDC55 gene. Together these data indicate that the Cdc55p/Zds2p module has a function in the regulation of the Swe1p-dependent G2/M checkpoint...
- Calmodulin controls organization of the actin cytoskeleton via regulation of phosphatidylinositol (4,5)-bisphosphate synthesis in Saccharomyces cerevisiaeSylvane Desrivieres
Division of Biochemistry, Biozentrum, University of Basel, Klingelbergstrasse 70, Switzerland
Biochem J 366:945-51. 2002..Finally, the cmd1-226 mutant exhibits reduced levels of phosphatidylinositol (4,5)-bisphosphate. These findings suggest that calmodulin positively controls MSS4 activity and thereby the actin cytoskeleton...
- Evidence for interaction of yeast protein kinase C with several subunits of oligosaccharyl transferaseH Park
Department of Biochemistry and Cell Biology, and Institute for Cell and Developmental Biology, SUNY at Stony Brook, 11794, USA
Glycobiology 10:737-44. 2000..experiments revealed that overexpression of two OT subunits rescued the growth defect caused by overexpression of a Pkc1 active site mutant, implying that there are specific genetic interactions between PKC1 and OT...
- Suppressors of a Saccharomyces cerevisiae pkc1 mutation identify alleles of the phosphatase gene PTC1 and of a novel gene encoding a putative basic leucine zipper proteinK N Huang
Institute of Cancer Research, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
Genetics 141:1275-85. 1995The PKC1 gene product, protein kinase C, regulates a mitogen-activated protein kinase (MAPK) cascade, which is implicated in cell wall metabolism...
- Pbp1p, a factor interacting with Saccharomyces cerevisiae poly(A)-binding protein, regulates polyadenylationD A Mangus
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0122, USA
Mol Cell Biol 18:7383-96. 1998..In the absence of Pbp1p, 3' termini of pre-mRNAs are properly cleaved but lack full-length poly(A) tails. These effects suggest that Pbp1p may act to repress the ability of Pab1p to negatively regulate polyadenylation...
- Identification of Kel1p, a kelch domain-containing protein involved in cell fusion and morphology in Saccharomyces cerevisiaeJ Philips
Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143 0448, USA
J Cell Biol 143:375-89. 1998..Coimmunoprecipitation and two-hybrid analyses indicated that Kel1p and Kel2p physically interact. We conclude that Kel1p has a role in cell morphogenesis and cell fusion and may antagonize the Pkc1p pathway...
- ROM7/BEM4 encodes a novel protein that interacts with the Rho1p small GTP-binding protein in Saccharomyces cerevisiaeH Hirano
Department of Molecular Biology and Biochemistry, Osaka University Medical School, Japan
Mol Cell Biol 16:4396-403. 1996..was suppressed by overexpression of RHO1, ROM2, which encodes a Rho1p-specific GDP/GTP exchange factor, or PKC1, which encodes a target of Rho1p...
- A screen for upstream components of the yeast protein kinase C signal transduction pathway identifies the product of the SLG1 geneJ J Jacoby
Institut fur Mikrobiologie, Heinrich Heine Universitat Dusseldorf, Germany
Mol Gen Genet 258:148-55. 1998..to caffeine can be partially suppressed by overexpression of genes for other components of the Pkc pathway, such as PKC1, SLT2, ROM2, and STE20...
- Dhh1p, a putative RNA helicase, associates with the general transcription factors Pop2p and Ccr4p from Saccharomyces cerevisiaeH Hata
Cellular Signaling Group, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan
Genetics 148:571-9. 1998..We have isolated five multicopy suppressor genes of a pop2 deletion mutation: CCR4, DHH1 (a putative RNA helicase), PKC1, STM1, and MPT5 (multicopy suppressor of pop two)...
- Protein-protein interactions in the yeast PKC1 pathway: Pkc1p interacts with a component of the MAP kinase cascadeG Paravicini
GLAXO Institue for Molecular Biology, Geneva, Switzerland
Mol Gen Genet 251:682-91. 1996..interactions was used to screen for proteins that interact in vivo with the Saccharomyces cerevisiae Pkc1 protein, a homolog of mammalian protein kinase C...
- A genetic screen reveals a role for the late G1-specific transcription factor Swi4p in diverse cellular functions including cytokinesisJ C Igual
Division of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK
J Cell Sci 110:1647-54. 1997..As overexpression or ectopic expression of CLN did not suppress the rsf1 swi4delta mutant phenotype, Swi4p must control some other gene(s) involved in cytokinesis...
- Functional interaction between the PKC1 pathway and CDC31 network of SPB duplication genesW Khalfan
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
Genetics 155:1543-59. 2000..Components of the PKC1 pathway were found to interact with these SPB duplication genes in two independent genetic screens...
- A novel gene, STT4, encodes a phosphatidylinositol 4-kinase in the PKC1 protein kinase pathway of Saccharomyces cerevisiaeS Yoshida
Department of Biology, Faculty of Science, University of Tokyo, Japan
J Biol Chem 269:1166-72. 1994A staurosporine-sensitive mutation (stt1) in yeast has been found in the PKC1 gene that encodes a protein kinase C homologue (Yoshida, S., Ikeda, E., Uno, I., and Mitsuzawa, H. (1992) Mol. Gen. Genet. 231, 337-344)...
- Calcineurin, the Ca2+/calmodulin-dependent protein phosphatase, is essential in yeast mutants with cell integrity defects and in mutants that lack a functional vacuolar H(+)-ATPaseP Garrett-Engele
Department of Biological Sciences, Stanford University, California 94305 5020
Mol Cell Biol 15:4103-14. 1995..Mutations in the genes for yeast protein kinase C (pkc1) and a MAP kinase (mpk1/slt2) disrupt a Ca(2+)-dependent signaling pathway required to maintain a normal cell wall ..
- Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathwayBob Chai
Department of Microbiology and Immunology and Morse Institute for Molecular Genetics, State University of New York, Brooklyn, New York 11203, USA
Genetics 161:575-84. 2002..Although the sth1-3ts mutant exhibits defects characteristic of PKC1 pathway mutants (caffeine and staurosporine sensitivities and an osmoremedial phenotype), only upstream components ..
- Regulation of yeast protein kinase C activity by interaction with the small GTPase Rho1p through its amino-terminal HR1 domainHans Peter Schmitz
Institut fur Mikrobiologie, Heinrich Heine Universitat Dusseldorf, Universitatsstr 1, Geb 26 12, Germany
Mol Microbiol 44:829-40. 2002..The intragenic lethality of mutants affected in both HR1A and the C1 domain reported in this work implies an essential role for Rho1p-Pkc1p interaction in yeast...
- Genetic interactions of yeast eukaryotic translation initiation factor 5A (eIF5A) reveal connections to poly(A)-binding protein and protein kinase C signalingSandro R Valentini
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
Genetics 160:393-405. 2002..the overexpression of which allows growth of a tif51A-1 strain at high temperature; these genes include PAB1, PKC1, and PKC1 regulators WSC1, WSC2, and WSC3...
- Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeastTobias Schmelzle
Department of Biochemistry, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
Mol Cell Biol 22:1329-39. 2002The PKC1-associated mitogen-activated protein (MAP) kinase pathway of Saccharomyces cerevisiae regulates cell integrity by controlling the actin cytoskeleton and cell wall synthesis...
- Characterization of the yeast (1-->6)-beta-glucan biosynthetic components, Kre6p and Skn1p, and genetic interactions between the PKC1 pathway and extracellular matrix assemblyT Roemer
Biology Department, McGill University, Montreal, Quebec, Canada
J Cell Biol 127:567-79. 1994..Deletion of the yeast protein kinase C homolog, PKC1, leads to a lysis defect (Levin, D. E., and E. Bartlett-Heubusch. 1992. Mutants in the S...
- Yeast Skn7p functions in a eukaryotic two-component regulatory pathwayJ L Brown
Department of Biology, McGill University, Montreal, Quebec, Canada
EMBO J 13:5186-94. 1994..Skn7p suppressed growth defects associated with a pkc1 delta mutation, raising the possibility that PKC1 might play a role in regulating Skn7p...
- The arrest of secretion response in yeast: signaling from the secretory path to the nucleus via Wsc proteins and Pkc1pJ Nanduri
Department of Pathology and Cell Biology Program, Case Western Reserve University, Cleveland, OH 44106, USA
Mol Cell 8:281-9. 2001..The activity of the downstream kinase, Pkc1p, is also required; however, the Pkc1p MAP kinase cascade is not. sec mutants initiate compensatory transcriptional changes distinct from those of the unfolded protein response...
- Coordinated regulation of gene expression by the cell cycle transcription factor Swi4 and the protein kinase C MAP kinase pathway for yeast cell integrityJ C Igual
Division of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK
EMBO J 15:5001-13. 1996..One of the isolated mutants, rsf8swi4delta, showed a growth defect due to cell lysis. rsf8 is allelic to PKC1, encoding a protein kinase C homologue which controls cell integrity...
- The hypo-osmolarity-sensitive phenotype of the Saccharomyces cerevisiae hpo2 mutant is due to a mutation in PKC1, which regulates expression of beta-glucanaseJ Shimizu
Central Research Laboratories, Asahi Breweries, Tokyo, Japan
Mol Gen Genet 242:641-8. 1994..Nine hypo-osmolarity-sensitive mutants were classified into two complementation groups, hpo1 and hpo2. Phase contrast microscopic observation showed that mutant cells bearing lesions at either locus became abnormally ..
- Down-regulation of Pkc1-mediated signaling by the deubiquitinating enzyme Ubp3Yuqi Wang
Department of Biology, Saint Louis University, St Louis, Missouri 63103, USA
J Biol Chem 283:1954-61. 2008..Here we provide evidence that in the yeast Saccharomyces cerevisiae, the Pkc1-mediated signaling pathway that controls the cell wall integrity is negatively regulated by the deubiquitinating ..
- Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth controlRobbie Loewith
Division of Biochemistry, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
Mol Cell 10:457-68. 2002..Thus, the distinct TOR complexes account for the diversity, specificity, and selective rapamycin inhibition of TOR signaling. TORC1 and possibly TORC2 are conserved from yeast to man...
- Saccharomyces cerevisiae YCRO17c/CWH43 encodes a putative sensor/transporter protein upstream of the BCK2 branch of the PKC1-dependent cell wall integrity pathwayH Martin-Yken
Centre de Bioingenierie Gilbert Durand, UMR CNRS 5504, UMR INRA 792, F 31077 Toulouse Cedex 04, France
Yeast 18:827-40. 2001..isolated for its Calcofluor white hypersensitivity, displays several cell wall defects similar to mutants in the PKC1-MPK1 pathway, including a growth defect and increased release of beta-1,6-glucan and beta-glucosylated proteins ..
- Mutations in SPC110, encoding the yeast spindle pole body calmodulin-binding protein, cause defects in cell integrity as well as spindle formationD A Stirling
Department of Biochemistry, University of Dundee, MSI WTB complex, DD1 5EH, Dundee, UK
Biochim Biophys Acta 1499:85-100. 2000..Our findings demonstrate a role for calcium in the Spc110p-calmodulin interaction in vivo and have important general implications for the interpretation of genetic interactions involving cell integrity genes...
- PtdIns(3)P accumulation in triple lipid-phosphatase-deletion mutants triggers lethal hyperactivation of the Rho1p/Pkc1p cell-integrity MAP kinase pathwayWilliam R Parrish
Department of Cellular and Molecular Medicine and the Howard Hughes Medical Institute, University of California at San Diego, School of Medicine, La Jolla, California 92093 0668, USA
J Cell Sci 118:5589-601. 2005..Interestingly, the most frequently isolated gene product was a truncated form of PKC1 (Pkc1-T615) that lacked the C-terminal kinase domain...
- Structure-function analysis of Knr4/Smi1, a newly member of intrinsically disordered proteins family, indispensable in the absence of a functional PKC1-SLT2 pathway in Saccharomyces cerevisiaeFabien Durand
University of Toulouse, INSA, UPS, INP and INRA, 135, Avenue de Rangeuil, F 31077, Toulouse, France
Yeast 25:563-76. 2008The coordination between cell wall synthesis and cell growth in the yeast Saccharomyces cerevisiae implicates the PKC1-dependent MAP kinase pathway...
- Calcofluor antifungal action depends on chitin and a functional high-osmolarity glycerol response (HOG) pathway: evidence for a physiological role of the Saccharomyces cerevisiae HOG pathway under noninducing conditionsL J García-Rodriguez
Instituto de Microbiologia Bioquimica, Consejo Superior de Investigaciones Cientificas Universidad de Salamanca, 37007 Salamanca, Spain
J Bacteriol 182:2428-37. 2000....
- Pkc1 and the upstream elements of the cell integrity pathway in Saccharomyces cerevisiae, Rom2 and Mtl1, are required for cellular responses to oxidative stressFelipe Vilella
Departament de Ciencies Mediques Basiques, Alcalde Rovira Roure 44, Universitat de Lleida, Lleida 25198, Spain
J Biol Chem 280:9149-59. 2005In this study we analyze the participation of the PKC1-MAPK cell integrity pathway in cellular responses to oxidative stress in Saccharomyces cerevisiae...
- PKC1, a protein kinase C homologue of Saccharomyces cerevisiae, participates in microtubule function through the yeast EB1 homologue, BIM1T Hosotani
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi Hiroshima, Japan 739 8527
Genes Cells 6:775-88. 2001..RSC is a chromatin-remodelling complex of Saccharomyces cerevisiae and essential for growth. Its catalytic subunit is encoded by the NPS1/STH1 gene. At the present time, little is known regarding the cellular function of RSC...
- Yeast MEK-dependent signal transduction: response thresholds and parameters affecting fidelityB Yashar
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599, USA
Mol Cell Biol 15:6545-53. 1995..This behavior suggests that Ste5p, which has been proposed to be a tether for the kinases in the mating pathway, contributes to Ste7p specificity...