PLA2G1B

Summary

Gene Symbol: PLA2G1B
Description: phospholipase A2, group IB (pancreas)
Alias: PLA2, sPLA(2)-IB, group IB phospholipase A2, group IB secretory phospholipase A(2), phosphatidylcholine 2-acylhydrolase 1B, phospholipase A2, major isoenzyme
Species: pig

Top Publications

  1. pmc Solution structure of porcine pancreatic phospholipase A2
    B van den Berg
    Center for Biomembranes and Lipid Enzymology, Utrecht University, The Netherlands
    EMBO J 14:4123-31. 1995
  2. doi Thermodynamic reciprocity of the inhibitor binding to the active site and the interface binding region of IB phospholipase A2
    Otto G Berg
    Department of Molecular Evolution, Uppsala University Evolutionary Biology Center, Uppsala, Sweden
    Biochemistry 48:3209-18. 2009
  3. ncbi Structure of porcine pancreatic phospholipase A2 at 2.6 A resolution and comparison with bovine phospholipase A2
    B W Dijkstra
    J Mol Biol 168:163-79. 1983
  4. ncbi NMR structures of phospholipase A2 reveal conformational changes during interfacial activation
    B van den Berg
    Department of Enzymology and Protein Engineering, Utrecht University, The Netherlands
    Nat Struct Biol 2:402-6. 1995
  5. ncbi Solution structure of porcine pancreatic phospholipase A2 complexed with micelles and a competitive inhibitor
    B van den Berg
    Center for Biomembranes and Lipid Enzymology, Utrecht University, The Netherlands
    J Biomol NMR 5:110-21. 1995
  6. ncbi Group IB secretory phospholipase A2 stimulates CXC chemokine ligand 8 production via ERK and NF-kappa B in human neutrophils
    Eun Jin Jo
    Medical Research Center for Cancer Molecular Therapy and Dong A University, Busan 602 714, Korea
    J Immunol 173:6433-9. 2004
  7. ncbi Distribution of reaction products in phospholipase A2 hydrolysis
    Hanna P Wacklin
    Oxford University, Physical and Theoretical Chemistry Laboratory, South Parks Road, Oxford OX1 3QZ, UK
    Biochim Biophys Acta 1768:1036-49. 2007
  8. pmc Structural basis for bile salt inhibition of pancreatic phospholipase A2
    Ying H Pan
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    J Mol Biol 369:439-50. 2007
  9. ncbi MMP production in human fibrosarcoma cells and their invasiveness are regulated by group IB secretory phospholipase A2 receptor-mediated activation of cytosolic phospholipase A2
    Michal Gorovetz
    Department of Pharmacology, Hebrew University School of Pharmacy, Hebrew University Hadassah Medical School, Jerusalem, Israel 91120
    Front Biosci 13:1917-25. 2008
  10. doi Contiguous binding of decylsulfate on the interface-binding surface of pancreatic phospholipase A2
    Shi Bai
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 47:2899-907. 2008

Scientific Experts

  • Hanna P Wacklin
  • Otto G Berg
  • Diethard R L Monbaliu
  • Mahendra K Jain
  • Shi Bai
  • Michal Gorovetz
  • Ying H Pan
  • B van den Berg
  • Eun Jin Jo
  • G H de Haas
  • Martine M J Crabbé
  • Bao Zhu Yu
  • Marcel M M Zeegers
  • Christel N T Dubuisson
  • Jacques M F Pirenne
  • Johan M J Fevery
  • Jos F van Pelt
  • Ouri Schwob
  • Saul Yedgar
  • Reuven Reich
  • Miron Krimsky
  • H M Verheij
  • Brian J Bahnson
  • R Boelens
  • R Kaptein
  • M Tessari
  • Yoe Sik Bae
  • Suk Hwan Baek
  • Dae Won Park
  • Hyun Kyu Kang
  • Youl Nam Lee
  • Ha Young Lee
  • Jung Im Kim
  • Jong Young Kwak
  • R Dijkman
  • B W Dijkstra
  • M M Thunnissen
  • K H Kalk
  • A J Slotboom
  • J Drenth
  • W C Puijk
  • J J Seilhamer
  • P De Geus
  • N Dekker
  • A G Tomasselli
  • P P Bonsen
  • L L van Deenen
  • A R Peters
  • L K Johnson
  • O Kuipers
  • E Ab
  • R Renetseder
  • C J Van den Bergh
  • G de Haas
  • J Fisher
  • E Oriaku
  • F J Kezdy
  • W Nieuwenhuizen
  • J Hui
  • W G Hol
  • H Zurcher-Neely
  • I M Reardon
  • M Yamanaka
  • O P Kuipers
  • R L Heinrikson
  • W P Hoekstra
  • T L Randall

Detail Information

Publications20

  1. pmc Solution structure of porcine pancreatic phospholipase A2
    B van den Berg
    Center for Biomembranes and Lipid Enzymology, Utrecht University, The Netherlands
    EMBO J 14:4123-31. 1995
    The lipolytic enzyme phospholipase A2 (PLA2) is involved in the degradation of high-molecular weight phospholipid aggregates in vivo...
  2. doi Thermodynamic reciprocity of the inhibitor binding to the active site and the interface binding region of IB phospholipase A2
    Otto G Berg
    Department of Molecular Evolution, Uppsala University Evolutionary Biology Center, Uppsala, Sweden
    Biochemistry 48:3209-18. 2009
    Interfacial activation of pig pancreatic IB phospholipase A(2) (PLA2) is modeled in terms of the three discrete premicellar complexes (E(i)(#), i = 1, 2, or 3) consecutively formed by the cooperative binding of a monodisperse amphiphile ..
  3. ncbi Structure of porcine pancreatic phospholipase A2 at 2.6 A resolution and comparison with bovine phospholipase A2
    B W Dijkstra
    J Mol Biol 168:163-79. 1983
    ..In addition to the calcium ion in the active site, a second calcium ion is present in the crystals; this is located on a crystallographic 2-fold axis and stabilizes the interaction between two neighbouring molecules...
  4. ncbi NMR structures of phospholipase A2 reveal conformational changes during interfacial activation
    B van den Berg
    Department of Enzymology and Protein Engineering, Utrecht University, The Netherlands
    Nat Struct Biol 2:402-6. 1995
    It has long been proposed that the higher activity of phospholipase A2 (PLA2) for substrates presented as multimolecular aggregates compared to dispersed molecules (interfacial activation) arises due to a conformational change in the ..
  5. ncbi Solution structure of porcine pancreatic phospholipase A2 complexed with micelles and a competitive inhibitor
    B van den Berg
    Center for Biomembranes and Lipid Enzymology, Utrecht University, The Netherlands
    J Biomol NMR 5:110-21. 1995
    The three-dimensional structure of porcine pancreatic PLA2 (PLA2), present in a 40 kDa ternary complex with micelles and a competitive inhibitor, has been determined using multidimensional heteronuclear NMR spectroscopy...
  6. ncbi Group IB secretory phospholipase A2 stimulates CXC chemokine ligand 8 production via ERK and NF-kappa B in human neutrophils
    Eun Jin Jo
    Medical Research Center for Cancer Molecular Therapy and Dong A University, Busan 602 714, Korea
    J Immunol 173:6433-9. 2004
    ..Taken together, we suggest that sPLA(2)-IB plays a role in the modulation of inflammatory and immune responses via the sPLA(2) receptor, by inducing CXCL8 in human neutrophils...
  7. ncbi Distribution of reaction products in phospholipase A2 hydrolysis
    Hanna P Wacklin
    Oxford University, Physical and Theoretical Chemistry Laboratory, South Parks Road, Oxford OX1 3QZ, UK
    Biochim Biophys Acta 1768:1036-49. 2007
    ..These are discussed in terms of a model of fatty-acid mediated activation of PLA(2)...
  8. pmc Structural basis for bile salt inhibition of pancreatic phospholipase A2
    Ying H Pan
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    J Mol Biol 369:439-50. 2007
    ..of amphipathic bile salts have been thought to interact with and activate the enzyme group-IB phospholipase A2 (PLA2) derived from the pancreas...
  9. ncbi MMP production in human fibrosarcoma cells and their invasiveness are regulated by group IB secretory phospholipase A2 receptor-mediated activation of cytosolic phospholipase A2
    Michal Gorovetz
    Department of Pharmacology, Hebrew University School of Pharmacy, Hebrew University Hadassah Medical School, Jerusalem, Israel 91120
    Front Biosci 13:1917-25. 2008
    ....
  10. doi Contiguous binding of decylsulfate on the interface-binding surface of pancreatic phospholipase A2
    Shi Bai
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 47:2899-907. 2008
    Pig pancreatic IB phospholipase A 2 (PLA2) forms three distinguishable premicellar E i (#) ( i = 1, 2, and 3) complexes at successively higher decylsulfate concentrations. The Hill coefficient for E 1 (#) is n 1 = 1...
  11. doi Increased serum phospholipase A2 activity after non-heart-beating donor liver transplantation and association with ischemia-reperfusion injury
    Diethard R L Monbaliu
    Department of Abdominal Transplant Surgery, University Hospitals Leuven, Leuven, Belgium
    J Surg Res 151:125-31. 2009
    ..A role for sPLA(2) in LTx from non-heart-beating donors (NHBD) has never been demonstrated. Furthermore data on the natural sPLA(2) inhibition capacity are scarce...
  12. ncbi The primary structure of phospholipase A2 from porcine pancreas. A reinvestigation
    W C Puijk
    Biochim Biophys Acta 492:254-9. 1977
    ..The structure which is presented here shows a high degree of homology with the amino acid sequence of snake venom and horse pancreas phospholipase A2...
  13. ncbi Studies on phospholipase A and its zymogen from porcine pancreas. I. The complete amino acid sequence
    G H de Haas
    Biochim Biophys Acta 221:31-53. 1970
  14. ncbi Studies on phospholipase A and its zymogen from porcine pancreas. II. The assignment of the position of the six disulfide bridges
    G H de Haas
    Biochim Biophys Acta 221:54-61. 1970
  15. pmc Expression of porcine pancreatic phospholipase A2. Generation of active enzyme by sequence-specific cleavage of a hybrid protein from Escherichia coli
    P De Geus
    Nucleic Acids Res 15:3743-59. 1987
    ..The released (pro)PLA was purified (yields of 2-3 mg/ltr of culture medium), and showed identical properties compared to native (pro)PLA...
  16. ncbi Pancreatic phospholipase A2: isolation of the human gene and cDNAs from porcine pancreas and human lung
    J J Seilhamer
    DNA 5:519-27. 1986
    Oligonucleotide probes synthesized using the published protein sequence for porcine pancreatic phospholipase A2 (PLA2; Puijk et al., 1977), were used to screen a cDNA library made from porcine pancreas...
  17. ncbi Dimerization and activation of porcine pancreatic phospholipase A2 via substrate level acylation of lysine 56
    A G Tomasselli
    Biopolymer Chemistry Unit, Upjohn Company, Kalamazoo, Michigan 49001
    J Biol Chem 264:10041-7. 1989
    ..L., and Kézdy, F. J. (1988) J. Biol. Chem. 263, 11237-11241), strongly support the proposal that interfacial activation of monomeric phospholipases is due to substrate-level autoacylation resulting in fully potentiated dimeric enzymes...
  18. ncbi Structure of an engineered porcine phospholipase A2 with enhanced activity at 2.1 A resolution. Comparison with the wild-type porcine and Crotalus atrox phospholipase A2
    M M Thunnissen
    Laboratory of Chemical Physics, University of Groningen, The Netherlands
    J Mol Biol 216:425-39. 1990
    ..In the mutant structure, this hydrophobic pocket is now occupied by the disulfide bond between residues 61 and 91. A detailed description of the second binding site for a calcium ion in this enzyme is given...
  19. ncbi X-ray structure of phospholipase A2 complexed with a substrate-derived inhibitor
    M M Thunnissen
    Laboratory of Chemical Physics, University of Groningen, The Netherlands
    Nature 347:689-91. 1990
    ....
  20. ncbi Porcine pancreatic phospholipase A2: sequence-specific 1H and 15N NMR assignments and secondary structure
    N Dekker
    Center for Biomembranes and Lipid Enzymology, State University of Utrecht, The Netherlands
    Biochemistry 30:3135-46. 1991
    ..This and the absence of several expected NOEs imply that this region has a less well defined structure in solution. Finally, the hydrogen bond between residues 78-81, which is part of a beta-sheet, does not show slow exchange behavior...