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| PLA2G1BSummaryGene Symbol: PLA2G1B Description: phospholipase A2, group IB (pancreas) Alias: PLA2, sPLA(2)-IB, group IB phospholipase A2, group IB secretory phospholipase A(2), phosphatidylcholine 2-acylhydrolase 1B, phospholipase A2, major isoenzyme Species: pig Top Publications
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Publications
Solution structure of porcine pancreatic phospholipase A2B van den Berg
Center for Biomembranes and Lipid Enzymology, Utrecht University, The Netherlands
EMBO J 14:4123-31. 1995The lipolytic enzyme phospholipase A2 (PLA2) is involved in the degradation of high-molecular weight phospholipid aggregates in vivo...
Thermodynamic reciprocity of the inhibitor binding to the active site and the interface binding region of IB phospholipase A2Otto G Berg
Department of Molecular Evolution, Uppsala University Evolutionary Biology Center, Uppsala, Sweden
Biochemistry 48:3209-18. 2009Interfacial activation of pig pancreatic IB phospholipase A(2) (PLA2) is modeled in terms of the three discrete premicellar complexes (E(i)(#), i = 1, 2, or 3) consecutively formed by the cooperative binding of a monodisperse amphiphile ..
Structure of porcine pancreatic phospholipase A2 at 2.6 A resolution and comparison with bovine phospholipase A2B W Dijkstra
J Mol Biol 168:163-79. 1983..In addition to the calcium ion in the active site, a second calcium ion is present in the crystals; this is located on a crystallographic 2-fold axis and stabilizes the interaction between two neighbouring molecules...- NMR structures of phospholipase A2 reveal conformational changes during interfacial activationB van den Berg
Department of Enzymology and Protein Engineering, Utrecht University, The Netherlands
Nat Struct Biol 2:402-6. 1995It has long been proposed that the higher activity of phospholipase A2 (PLA2) for substrates presented as multimolecular aggregates compared to dispersed molecules (interfacial activation) arises due to a conformational change in the .. - Solution structure of porcine pancreatic phospholipase A2 complexed with micelles and a competitive inhibitorB van den Berg
Center for Biomembranes and Lipid Enzymology, Utrecht University, The Netherlands
J Biomol NMR 5:110-21. 1995The three-dimensional structure of porcine pancreatic PLA2 (PLA2), present in a 40 kDa ternary complex with micelles and a competitive inhibitor, has been determined using multidimensional heteronuclear NMR spectroscopy... - Group IB secretory phospholipase A2 stimulates CXC chemokine ligand 8 production via ERK and NF-kappa B in human neutrophilsEun Jin Jo
Medical Research Center for Cancer Molecular Therapy and Dong A University, Busan 602 714, Korea
J Immunol 173:6433-9. 2004..Taken together, we suggest that sPLA(2)-IB plays a role in the modulation of inflammatory and immune responses via the sPLA(2) receptor, by inducing CXCL8 in human neutrophils... - Distribution of reaction products in phospholipase A2 hydrolysisHanna P Wacklin
Oxford University, Physical and Theoretical Chemistry Laboratory, South Parks Road, Oxford OX1 3QZ, UK
Biochim Biophys Acta 1768:1036-49. 2007..These are discussed in terms of a model of fatty-acid mediated activation of PLA(2)... - Structural basis for bile salt inhibition of pancreatic phospholipase A2Ying H Pan
Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
J Mol Biol 369:439-50. 2007..of amphipathic bile salts have been thought to interact with and activate the enzyme group-IB phospholipase A2 (PLA2) derived from the pancreas... - MMP production in human fibrosarcoma cells and their invasiveness are regulated by group IB secretory phospholipase A2 receptor-mediated activation of cytosolic phospholipase A2Michal Gorovetz
Department of Pharmacology, Hebrew University School of Pharmacy, Hebrew University Hadassah Medical School, Jerusalem, Israel 91120
Front Biosci 13:1917-25. 2008.... - Contiguous binding of decylsulfate on the interface-binding surface of pancreatic phospholipase A2Shi Bai
Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
Biochemistry 47:2899-907. 2008Pig pancreatic IB phospholipase A 2 (PLA2) forms three distinguishable premicellar E i (#) ( i = 1, 2, and 3) complexes at successively higher decylsulfate concentrations. The Hill coefficient for E 1 (#) is n 1 = 1... - Increased serum phospholipase A2 activity after non-heart-beating donor liver transplantation and association with ischemia-reperfusion injuryDiethard R L Monbaliu
Department of Abdominal Transplant Surgery, University Hospitals Leuven, Leuven, Belgium
J Surg Res 151:125-31. 2009..A role for sPLA(2) in LTx from non-heart-beating donors (NHBD) has never been demonstrated. Furthermore data on the natural sPLA(2) inhibition capacity are scarce... - The primary structure of phospholipase A2 from porcine pancreas. A reinvestigationW C Puijk
Biochim Biophys Acta 492:254-9. 1977..The structure which is presented here shows a high degree of homology with the amino acid sequence of snake venom and horse pancreas phospholipase A2... - Studies on phospholipase A and its zymogen from porcine pancreas. I. The complete amino acid sequenceG H de Haas
Biochim Biophys Acta 221:31-53. 1970 - Studies on phospholipase A and its zymogen from porcine pancreas. II. The assignment of the position of the six disulfide bridgesG H de Haas
Biochim Biophys Acta 221:54-61. 1970 - Expression of porcine pancreatic phospholipase A2. Generation of active enzyme by sequence-specific cleavage of a hybrid protein from Escherichia coliP De Geus
Nucleic Acids Res 15:3743-59. 1987..The released (pro)PLA was purified (yields of 2-3 mg/ltr of culture medium), and showed identical properties compared to native (pro)PLA... - Pancreatic phospholipase A2: isolation of the human gene and cDNAs from porcine pancreas and human lungJ J Seilhamer
DNA 5:519-27. 1986Oligonucleotide probes synthesized using the published protein sequence for porcine pancreatic phospholipase A2 (PLA2; Puijk et al., 1977), were used to screen a cDNA library made from porcine pancreas... - Dimerization and activation of porcine pancreatic phospholipase A2 via substrate level acylation of lysine 56A G Tomasselli
Biopolymer Chemistry Unit, Upjohn Company, Kalamazoo, Michigan 49001
J Biol Chem 264:10041-7. 1989..L., and Kézdy, F. J. (1988) J. Biol. Chem. 263, 11237-11241), strongly support the proposal that interfacial activation of monomeric phospholipases is due to substrate-level autoacylation resulting in fully potentiated dimeric enzymes... - Structure of an engineered porcine phospholipase A2 with enhanced activity at 2.1 A resolution. Comparison with the wild-type porcine and Crotalus atrox phospholipase A2M M Thunnissen
Laboratory of Chemical Physics, University of Groningen, The Netherlands
J Mol Biol 216:425-39. 1990..In the mutant structure, this hydrophobic pocket is now occupied by the disulfide bond between residues 61 and 91. A detailed description of the second binding site for a calcium ion in this enzyme is given... - X-ray structure of phospholipase A2 complexed with a substrate-derived inhibitorM M Thunnissen
Laboratory of Chemical Physics, University of Groningen, The Netherlands
Nature 347:689-91. 1990.... - Porcine pancreatic phospholipase A2: sequence-specific 1H and 15N NMR assignments and secondary structureN Dekker
Center for Biomembranes and Lipid Enzymology, State University of Utrecht, The Netherlands
Biochemistry 30:3135-46. 1991..This and the absence of several expected NOEs imply that this region has a less well defined structure in solution. Finally, the hydrogen bond between residues 78-81, which is part of a beta-sheet, does not show slow exchange behavior...