INS

Summary

Gene Symbol: INS
Description: insulin
Alias: insulin, preproinsulin, proinsulin
Species: pig
Products:     INS

Top Publications

  1. Boutchueng Djidjou M, Collard Simard G, Fortier S, Hébert S, Kelly I, Landry C, et al. The last enzyme of the de novo purine synthesis pathway 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) plays a central role in insulin signaling and the Golgi/endosomes protein network. Mol Cell Proteomics. 2015;14:1079-92 pubmed publisher
    b>Insulin is internalized with its cognate receptor into the endosomal apparatus rapidly after binding to hepatocytes...
  2. Greaves R, Dodson G, Verma C. Altered ionization of the B13 Glu in insulin B9 and B10 mutants: a computational analysis. Protein Eng Des Sel. 2004;17:557-63 pubmed
    ..50 units has been reported for the B13 Glu residue in a dimeric B9 Ser --> Asp insulin mutant relative to the native dimer...
  3. Bou M, Todorcević M, Rodriguez J, Capilla E, Gutierrez J, Navarro I. Interplay of adiponectin, TNF? and insulin on gene expression, glucose uptake and PPAR?, AKT and TOR pathways in rainbow trout cultured adipocytes. Gen Comp Endocrinol. 2014;205:218-25 pubmed publisher
    ..trout adipocytes, obtained from visceral adipose tissue, the interplay of the adiponectin system, TNF? and insulin at a transcriptional level and, their effects on the adipogenic transcription factor PPAR?, as well as on the ..
  4. Amarger V, Nguyen M, Van Laere A, Braunschweig M, Nezer C, Georges M, et al. Comparative sequence analysis of the INS-IGF2-H19 gene cluster in pigs. Mamm Genome. 2002;13:388-98 pubmed
    ..Here we report two sequence contigs together comprising almost 90 kb containing the INS-IGF2 and H19 genes...
  5. Pandini G, Frasca F, Mineo R, Sciacca L, Vigneri R, Belfiore A. Insulin/insulin-like growth factor I hybrid receptors have different biological characteristics depending on the insulin receptor isoform involved. J Biol Chem. 2002;277:39684-95 pubmed
    The insulin receptor (IR) and the insulin-like growth factor I receptor (IGF-IR) have a highly homologous structure, but different biological effects...
  6. Badger J, Harris M, Reynolds C, Evans A, Dodson E, Dodson G, et al. Structure of the pig insulin dimer in the cubic crystal. Acta Crystallogr B. 1991;47 ( Pt 1):127-36 pubmed
    Atomic coordinates for pig insulin in the cubic crystal have been refined by reciprocal-space methods to an R factor of 0.173 for data between 10.0 and 1...
  7. Reynolds C, Elias A, Whisnant C. Effects of feeding pattern on ghrelin and insulin secretion in pigs. Domest Anim Endocrinol. 2010;39:90-6 pubmed publisher
    ..Plasma concentrations of ghrelin and insulin were determined by radioimmunoassay...
  8. Wientjes J, Soede N, Aarsse F, Laurenssen B, Koopmanschap R, Van Den Brand H, et al. Effects of dietary carbohydrate sources on plasma glucose, insulin and IGF-I levels in multiparous sows. J Anim Physiol Anim Nutr (Berl). 2012;96:494-505 pubmed publisher
    Effects of different carbohydrate sources on plasma glucose, insulin and insulin-like growth factor-I (IGF-I) levels were compared to subsequently be able to study effects of insulin-stimulating diets on follicle development in sows...
  9. Diao J, Wan Z, Chang W, Liang D. Structure of monomeric porcine DesB1-B2 despentapeptide (B26-B30) insulin at 1.65 A resolution. Acta Crystallogr D Biol Crystallogr. 1997;53:507-12 pubmed
    b>Insulin has a concentration of 10(-8)-10(-11) M in the blood which ensures that it circulates and exerts its physiological functions in vivo as a monomer...
  10. Dehoff M, Elgin R, Collier R, Clemmons D. Both type I and II insulin-like growth factor receptor binding increase during lactogenesis in bovine mammary tissue. Endocrinology. 1988;122:2412-7 pubmed
    Bovine GH is a potent stimulant of lactation, and the insulin-like growth factors I and II (IGF-I and -II) are believed to mediate GH's growth-promoting actions...

Detail Information

Publications36

  1. Boutchueng Djidjou M, Collard Simard G, Fortier S, Hébert S, Kelly I, Landry C, et al. The last enzyme of the de novo purine synthesis pathway 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) plays a central role in insulin signaling and the Golgi/endosomes protein network. Mol Cell Proteomics. 2015;14:1079-92 pubmed publisher
    b>Insulin is internalized with its cognate receptor into the endosomal apparatus rapidly after binding to hepatocytes...
  2. Greaves R, Dodson G, Verma C. Altered ionization of the B13 Glu in insulin B9 and B10 mutants: a computational analysis. Protein Eng Des Sel. 2004;17:557-63 pubmed
    ..50 units has been reported for the B13 Glu residue in a dimeric B9 Ser --> Asp insulin mutant relative to the native dimer...
  3. Bou M, Todorcević M, Rodriguez J, Capilla E, Gutierrez J, Navarro I. Interplay of adiponectin, TNF? and insulin on gene expression, glucose uptake and PPAR?, AKT and TOR pathways in rainbow trout cultured adipocytes. Gen Comp Endocrinol. 2014;205:218-25 pubmed publisher
    ..trout adipocytes, obtained from visceral adipose tissue, the interplay of the adiponectin system, TNF? and insulin at a transcriptional level and, their effects on the adipogenic transcription factor PPAR?, as well as on the ..
  4. Amarger V, Nguyen M, Van Laere A, Braunschweig M, Nezer C, Georges M, et al. Comparative sequence analysis of the INS-IGF2-H19 gene cluster in pigs. Mamm Genome. 2002;13:388-98 pubmed
    ..Here we report two sequence contigs together comprising almost 90 kb containing the INS-IGF2 and H19 genes...
  5. Pandini G, Frasca F, Mineo R, Sciacca L, Vigneri R, Belfiore A. Insulin/insulin-like growth factor I hybrid receptors have different biological characteristics depending on the insulin receptor isoform involved. J Biol Chem. 2002;277:39684-95 pubmed
    The insulin receptor (IR) and the insulin-like growth factor I receptor (IGF-IR) have a highly homologous structure, but different biological effects...
  6. Badger J, Harris M, Reynolds C, Evans A, Dodson E, Dodson G, et al. Structure of the pig insulin dimer in the cubic crystal. Acta Crystallogr B. 1991;47 ( Pt 1):127-36 pubmed
    Atomic coordinates for pig insulin in the cubic crystal have been refined by reciprocal-space methods to an R factor of 0.173 for data between 10.0 and 1...
  7. Reynolds C, Elias A, Whisnant C. Effects of feeding pattern on ghrelin and insulin secretion in pigs. Domest Anim Endocrinol. 2010;39:90-6 pubmed publisher
    ..Plasma concentrations of ghrelin and insulin were determined by radioimmunoassay...
  8. Wientjes J, Soede N, Aarsse F, Laurenssen B, Koopmanschap R, Van Den Brand H, et al. Effects of dietary carbohydrate sources on plasma glucose, insulin and IGF-I levels in multiparous sows. J Anim Physiol Anim Nutr (Berl). 2012;96:494-505 pubmed publisher
    Effects of different carbohydrate sources on plasma glucose, insulin and insulin-like growth factor-I (IGF-I) levels were compared to subsequently be able to study effects of insulin-stimulating diets on follicle development in sows...
  9. Diao J, Wan Z, Chang W, Liang D. Structure of monomeric porcine DesB1-B2 despentapeptide (B26-B30) insulin at 1.65 A resolution. Acta Crystallogr D Biol Crystallogr. 1997;53:507-12 pubmed
    b>Insulin has a concentration of 10(-8)-10(-11) M in the blood which ensures that it circulates and exerts its physiological functions in vivo as a monomer...
  10. Dehoff M, Elgin R, Collier R, Clemmons D. Both type I and II insulin-like growth factor receptor binding increase during lactogenesis in bovine mammary tissue. Endocrinology. 1988;122:2412-7 pubmed
    Bovine GH is a potent stimulant of lactation, and the insulin-like growth factors I and II (IGF-I and -II) are believed to mediate GH's growth-promoting actions...
  11. Qiao Z, Guo Z, Feng Y. In vitro folding/unfolding of insulin/single-chain insulin. Protein Pept Lett. 2006;13:423-9 pubmed
    ..the in vitro oxidative folding process of two single-chain insulins: porcine insulin precursor (PIP) and human proinsulin (HPI)...
  12. Tang Y, Wang S, Chen Y, Xu G, Feng Y. In vitro insulin refolding: characterization of the intermediates and the putative folding pathway. Sci China C Life Sci. 2007;50:717-25 pubmed
    The in vitro refolding process of the double-chain insulin was studied based on the investigation of in vitro single-chain insulin refolding...
  13. Yang S, Xu C, Wu J, Yang G. SOCS3 inhibits insulin signaling in porcine primary adipocytes. Mol Cell Biochem. 2010;345:45-52 pubmed publisher
    b>Insulin resistance is a major player in the pathogenesis of type II diabetes, the metabolic syndrome, and obesity. SOCS3 plays an important role in the development of insulin resistance...
  14. Van Laere A, Nguyen M, Braunschweig M, Nezer C, Collette C, Moreau L, et al. A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature. 2003;425:832-6 pubmed
    ..paternally expressed QTL affecting muscle growth, fat deposition and size of the heart in pigs maps to the IGF2 (insulin-like growth factor 2) region...
  15. Whittingham J, Chaudhuri S, Dodson E, Moody P, Dodson G. X-ray crystallographic studies on hexameric insulins in the presence of helix-stabilizing agents, thiocyanate, methylparaben, and phenol. Biochemistry. 1995;34:15553-63 pubmed
    Three X-ray crystallographic studies have been carried out on pig insulin in the presence of three ligands, thiocyanate, methylparaben (methyl p-hydroxybenzoate), and phenol...
  16. Ellis L, Clauser E, Morgan D, Edery M, Roth R, Rutter W. Replacement of insulin receptor tyrosine residues 1162 and 1163 compromises insulin-stimulated kinase activity and uptake of 2-deoxyglucose. Cell. 1986;45:721-32 pubmed
    b>Insulin stimulates the autophosphorylation of tyrosine residues of the beta subunit of the insulin receptor (IR); this modified insulin-independent kinase has increased activity toward exogenous substrates in vitro...
  17. Xu B, Huang K, Chu Y, Hu S, Nakagawa S, Wang S, et al. Decoding the cryptic active conformation of a protein by synthetic photoscanning: insulin inserts a detachable arm between receptor domains. J Biol Chem. 2009;284:14597-608 pubmed publisher
    ..A model is provided by insulin, a vertebrate hormone central to the control of metabolism...
  18. Iwai W, Yamada T, Kurihara K, Ohnishi Y, Kobayashi Y, Tanaka I, et al. A neutron crystallographic analysis of T6 porcine insulin at 2.1 A resolution. Acta Crystallogr D Biol Crystallogr. 2009;65:1042-50 pubmed publisher
    Neutron diffraction data for T(6) porcine insulin were collected to 2.1 A resolution from a single crystal partly deuterated by exchange of mother liquor...
  19. Babenko V, Dzwolak W. Amino acid sequence determinants in self-assembly of insulin chiral amyloid superstructures: role of C-terminus of B-chain in association of fibrils. FEBS Lett. 2013;587:625-30 pubmed publisher
    ..amyloid superstructures is a newly recognised phenomenon observed upon agitation-assisted fibrillation of bovine insulin. Here, by surveying several amyloidogenic precursors we examine whether formation of such entities is unique to ..
  20. Li Y, Huang L, Yang X, Wang C, Sun Y, Gong H, et al. The effect of exposing a critical hydrophobic patch on amyloidogenicity and fibril structure of insulin. Biochem Biophys Res Commun. 2013;440:56-61 pubmed publisher
    ..In this study, insulin was used as a model to investigate the effect of exposing a critical hydrophobic patch on amyloidogenicity and ..
  21. Renner S, Braun Reichhart C, Blutke A, Herbach N, Emrich D, Streckel E, et al. Permanent neonatal diabetes in INS(C94Y) transgenic pigs. Diabetes. 2013;62:1505-11 pubmed publisher
    Mutations in the insulin (INS) gene may cause permanent neonatal diabetes mellitus (PNDM). Ins2 mutant mouse models provided important insights into the disease mechanisms of PNDM but have limitations for translational research...
  22. Lisi G, Png C, Wilcox D. Thermodynamic contributions to the stability of the insulin hexamer. Biochemistry. 2014;53:3576-84 pubmed publisher
    The insulin hexamer is resistant to degradation and fibrillation, which makes it an important quaternary structure for its in vivo storage in Zn(2+)- and Ca(2+)-rich vesicles in the pancreas and for pharmaceutical formulations...
  23. Singh R, Bansal R, Rathore A, Goel G. Equilibrium Ensembles for Insulin Folding from Bias-Exchange Metadynamics. Biophys J. 2017;112:1571-1585 pubmed publisher
    ..we have used well-tempered bias exchange metadynamics simulations to determine the equilibrium ensembles of an insulin molecule under amyloidogenic conditions of low pH and high temperature...
  24. Harb G, Toreson J, Dufour J, Korbutt G. Acute exposure to streptozotocin but not human proinflammatory cytokines impairs neonatal porcine islet insulin secretion in vitro but not in vivo. Xenotransplantation. 2007;14:580-90 pubmed
    ..In vitro exposure to STZ, but not cytokines, significantly reduced NPI glucose stimulated insulin secretion (1.1 +/- 0.1 vs. 4.3 +/- 1.3-fold stimulation index in STZ vs. control, P < 0...
  25. Segner H, Blair J, Wirtz G, Miller M. Cultured trout liver cells: utilization of substrates and response to hormones. In Vitro Cell Dev Biol Anim. 1994;30A:306-11 pubmed
    ..dihydroxyacetone, and glycerol for glucose and lactate formation; b) the effects of the pancreatic hormones insulin and glucagon on cellular glucose formation, lactate formation, and fatty acid synthesis; and c) the effects of ..
  26. Chance R, Ellis R, Bromer W. Porcine proinsulin: characterization and amino acid sequence. Science. 1968;161:165-7 pubmed
    b>Proinsulin in nearly homogeneous form has been isolated from a preparation of porcine insulin. A molecular weight close to 9100 was calculated from the amino acid composition and from sedimentation-equilibrium studies...
  27. Grzech M, Dahlhoff M, Herbach N, Habermann F, Renner Müller I, Wanke R, et al. Specific transgene expression in mouse pancreatic beta-cells under the control of the porcine insulin promoter. Mol Cell Endocrinol. 2010;315:219-24 pubmed publisher
    ..The rat insulin 2 gene (Ins2) promoter, widely used to achieve transgene expression in pancreatic beta-cells of mice, also ..
  28. Zhang Z, Wu L, Qiao Z, Qiao M, Feng Y, Guo Z. Contribution of the conserved A16Leu to insulin foldability. Protein J. 2008;27:192-6 pubmed
    Contributions of the evolutionarily conserved A16Leu and B17Leu to insulin foldability were characterized by evaluating folding properties of single-chain insulin analogs...
  29. Langston J, Li W, Harrison L, Aw T. Activation of promoter activity of the catalytic subunit of ?-glutamylcysteine ligase (GCL) in brain endothelial cells by insulin requires antioxidant response element 4 and altered glycemic status: implication for GCL expression and GSH synthesis. Free Radic Biol Med. 2011;51:1749-57 pubmed publisher
    Our recent finding that insulin increased the expression of the glutamate-cysteine ligase catalytic subunit (GCLc) with coincident increases in GCL activity and cellular glutathione (GSH) in human brain microvascular endothelial cells (..
  30. Liu X, Ruan J, Xia J, Yang S, Fan J, Li K. The study of regulatory effects of Pdx-1, MafA and NeuroD1 on the activity of porcine insulin promoter and the expression of human islet amyloid polypeptide. Mol Cell Biochem. 2014;394:59-66 pubmed publisher
    The purpose of the present study was to determine the activation of porcine insulin promoter (PIP) by three transcription factors: pancreatic and duodenal homeobox 1 (Pdx-1), v-maf musculoaponeurotic fibrosarcoma oncogene (MafA) and ..
  31. De W, Ai rong Z, Yan L, Sheng yu X, Hai yan G, Yong Z. Effect of feeding allowance level on embryonic survival, IGF-1, insulin, GH, leptin and progesterone secretion in early pregnancy gilts. J Anim Physiol Anim Nutr (Berl). 2009;93:577-85 pubmed publisher
    ..Concentrations of insulin-like growth factor I (IGF)-1, insulin, growth harmone (GH), leptin and progesterone in serum and uterine ..
  32. Sasaoka T, Rose D, Jhun B, Saltiel A, Draznin B, Olefsky J. Evidence for a functional role of Shc proteins in mitogenic signaling induced by insulin, insulin-like growth factor-1, and epidermal growth factor. J Biol Chem. 1994;269:13689-94 pubmed
    Shc proteins contain a single SH2 domain, lack catalytic activity, and are substrates for activated receptors for insulin, insulin-like growth factor-1 (IGF-1), and epidermal growth factor (EGF)...
  33. Baker E, Blundell T, Cutfield J, Cutfield S, Dodson E, Dodson G, et al. The structure of 2Zn pig insulin crystals at 1.5 A resolution. Philos Trans R Soc Lond B Biol Sci. 1988;319:369-456 pubmed
    The paper describes the arrangement of the atoms within rhombohedral crystals of 2Zn pig insulin as seen in electron density maps calculated from X-ray data extending to 1...
  34. Shahidullah M, Mandal A, Delamere N. Src Family Kinase Links Insulin Signaling to Short Term Regulation of Na,K-ATPase in Nonpigmented Ciliary Epithelium. J Cell Physiol. 2017;232:1489-1500 pubmed publisher
    b>Insulin has been shown to elicit changes of Na,K-ATPase activity in various tissues...
  35. Balschmidt P, Hansen F, Dodson E, Dodson G, Korber F. Structure of porcine insulin cocrystallized with clupeine Z. Acta Crystallogr B. 1991;47 ( Pt 6):975-86 pubmed
    The crystal structure of NPH-insulin, pig insulin cocrystallized with zinc, m-cresol and protamine, has been solved by molecular replacement and refined using restrained least-squares refinement methods...
  36. Argetsinger L, Hsu G, Myers M, Billestrup N, White M, Carter Su C. Growth hormone, interferon-gamma, and leukemia inhibitory factor promoted tyrosyl phosphorylation of insulin receptor substrate-1. J Biol Chem. 1995;270:14685-92 pubmed
    ..In the present study, GH is shown to stimulate tyrosyl phosphorylation of insulin receptor substrate 1 (IRS-1), the principle substrate of the insulin receptor...