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Genomes and Genes | ompASummaryGene Symbol: ompA Description: outer membrane protein A (3a;II*;G;d) Alias: ECK0948, JW0940, con, tolG, tut Species: Top Publications
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The structure of SecB/OmpA as visualized by electron microscopy: The mature region of the precursor protein binds asymmetrically to SecBYing Tang
State Key Laboratory of Biomembrane and Membrane Biotechnology, Center for Structural Biology, School of Life Science, Tsinghua University, Beijing 100084, China
Biochem Biophys Res Commun 393:698-702. 2010..the binding pattern of SecB and the mature region of the preprotein, here, we visualized the structure of the SecB/OmpA complex by electron microscopy...- Escherichia coli interaction with human brain microvascular endothelial cells induces signal transducer and activator of transcription 3 association with the C-terminal domain of Ec-gp96, the outer membrane protein A receptor for invasionRavi Maruvada
Division of Infectious Diseases, The Saban Research Institute, Childrens Hospital Los Angeles, CA 90027, USA
Cell Microbiol 10:2326-38. 2008..Together, our findings identified a novel interaction of Stat3 with Ec-gp96, upstream of PI3-kinase and PKC-alpha activation that is required for the invasion of E. coli into HBMEC... - Proteomic analysis of Escherichia coli biofilms reveals the overexpression of the outer membrane protein OmpARowan Orme
University of Manchester, Faculty of Medicine and Human Health, Centre for Molecular Medicine, Department of Medical Genetics, Manchester, UK
Proteomics 6:4269-77. 2006..report we have used proteomic analysis coupled with immunoassays to show that the major outer membrane protein (OmpA) of Escherichia coli is overexpressed during biofilm formation... - Conserved small non-coding RNAs that belong to the sigmaE regulon: role in down-regulation of outer membrane proteinsJesper Johansen
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230, Odense M, Denmark
J Mol Biol 364:1-8. 2006..Recently MicA was shown to act in destabilizing the ompA transcript when rapidly grown cells entered the stationary phase of growth... - A naturally occurring novel allele of Escherichia coli outer membrane protein A reduces sensitivity to bacteriophageMichelle L Power
Department of Biological Sciences, Macquarie University, North Ryde, Sydney, NSW 2109, Australia
Appl Environ Microbiol 72:7930-2. 2006A novel Escherichia coli outer membrane protein A (OmpA) was discovered through a proteomic investigation of cell surface proteins... - The ompA 5' untranslated RNA segment functions in Escherichia coli as a growth-rate-regulated mRNA stabilizer whose activity is unrelated to translational efficiencyS A Emory
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
J Bacteriol 172:4472-81. 1990The 5' untranslated region (UTR) of the long-lived Escherichia coli ompA message can function in vivo as an mRNA stabilizer... - Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAsIsabella Moll
Department of Microbiology and Genetics, University Departments at the Vienna Biocenter, Dr Bohrgasse 9, 1030 Vienna, Austria
RNA 9:1308-14. 2003..More recent work suggested a role of Hfq in cellular physiology through its interaction with ompA mRNA and small RNAs (sRNAs), some of which are involved in translational regulation... - Differential effects of yfgL mutation on Escherichia coli outer membrane proteins and lipopolysaccharideEmily S Charlson
School of Life Sciences, Arizona State University, Tempe, AZ 85287 4501, USA
J Bacteriol 188:7186-94. 2006..Without YfgL, the levels of OmpA, OmpF, and LamB are significantly reduced, while OmpC levels are slightly reduced... - Down-regulation of porins by a small RNA bypasses the essentiality of the regulated intramembrane proteolysis protease RseP in Escherichia coliVéronique Douchin
Signalisation et Réseaux de Régulations Bactériens, Institut de Genetique et Microbiologie, CNRS UMR8621 IFR115, Centre Scientifique d Orsay, Universite Paris Sud, 91405 Orsay Cedex, France
J Biol Chem 281:12253-9. 2006..We used the property that small RNAs are often involved in RNA-RNA interactions to capture RseX putative partners; ompA and ompC mRNA, which encode two major outer membrane proteins, were identified... - The targeting pathway of Escherichia coli presecretory and integral membrane proteins is specified by the hydrophobicity of the targeting signalH C Lee
Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 10, Room 9D 20, Bethesda, MD 20892 1810, USA
Proc Natl Acad Sci U S A 98:3471-6. 2001..have demonstrated that presecretory proteins such as maltose binding protein (MBP) and outer membrane protein A (OmpA) are targeted to the Escherichia coli inner membrane by the molecular chaperone SecB, but that integral membrane .. - Regulation of ompA mRNA stability: the role of a small regulatory RNA in growth phase-dependent controlAnders Aamann Rasmussen
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
Mol Microbiol 58:1421-9. 2005The Escherichia coli ompA mRNA, encoding a highly abundant outer membrane protein, has served as a model for regulated mRNA decay in bacteria... - Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNAKlas I Udekwu
Department of Cell and Molecular Biology, Uppsala University, S 75124 Uppsala, Sweden
Genes Dev 19:2355-66. 2005This paper shows that the small RNA MicA (previously SraD) is an antisense regulator of ompA in Escherichia coli. MicA accumulates upon entry into stationary phase and down-regulates the level of ompA mRNA... - Nucleotide sequence of the gene ompA coding the outer membrane protein II of Escherichia coli K-12E Beck
Nucleic Acids Res 8:3011-27. 1980A nucleotide sequence of 2271 basepairs has been determined from cloned E. coli DNA which contains ompA. Withing that sequence, starting at nucleotide 1037, an open translational reading frame encodes a protein of 367 amino acids which .. - Simultaneous force and fluorescence measurements of a protein that forms a bond between a living bacterium and a solid surfaceBrian H Lower
Pacific Northwest National Laboratory, Richland, Washington, USA
J Bacteriol 187:2127-37. 2005..The chimera was composed of a portion of outer membrane protein A (OmpA) fused to the cyan-fluorescent protein AmCyan... - MD simulations of spontaneous membrane protein/detergent micelle formationPeter J Bond
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
J Am Chem Soc 126:15948-9. 2004..formation around representatives of the two major families of membrane proteins, a small beta-barrel protein, OmpA, and a model alpha-helical protein, glycophorin A... - Phage-induced change in the stability of mRNAsHiroyuki Ueno
Department of Biology, Graduate School of Science, Osaka University, 1 1 Machikaneyama cho, Toyonaka shi, Osaka 560 0043, Japan
Virology 329:134-41. 2004..Stable E. coli mRNAs such as lpp and ompA were drastically destabilized immediately after infection... - Improved methods for producing outer membrane vesicles in Gram-negative bacteriaThomas Henry
Centre d immunologie de Marseille Luminy, Parc Scientifique de Luminy, Case 906, 13288 Marseille 9, France
Res Microbiol 155:437-46. 2004..coli or Shigella flexneri cell envelope and to induce production of high amounts of vesicles. This technique was further found to work efficiently in Salmonella enterica serovar Typhimurium... - A molecular phylogeny of enteric bacteria and implications for a bacterial species conceptJ E Wertz
Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06511, USA
J Evol Biol 16:1236-48. 2003..Sequences from five housekeeping genes (gapA, groEL, gyrA, ompA, and pgi) and the 16S rRNA gene were used to infer individual gene trees and were concatenated to infer a composite .. - Temperature-dependent stability and translation of Escherichia coli ompA mRNATaras Afonyushkin
Department of Microbiology and Genetics, University of Vienna, Dr Bohrgasse 9 4, A 1030 Vienna, Austria
Biochem Biophys Res Commun 311:604-9. 2003RNase E is known to affect the turnover of ompA mRNA in a growth rate-dependent manner... - YidC is strictly required for membrane insertion of subunits a and c of the F(1)F(0)ATP synthase and SecE of the SecYEG translocaseLiang Yi
Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
Biochemistry 42:10537-44. 2003..In contrast, we found that protein export of OmpA is severely blocked at 25 degrees C when YidC is depleted, which may be due to the decreased SecE level, as a 50% .. - Outer membrane protein A and cytotoxic necrotizing factor-1 use diverse signaling mechanisms for Escherichia coli K1 invasion of human brain microvascular endothelial cellsNaveed Ahmed Khan
Division of Pediatric Infectious Diseases, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
Microb Pathog 35:35-42. 2003..We previously have shown that outer membrane protein A (OmpA) and cytotoxic necrotizing factor-1 (CNF1) contribute to E. coli K1 invasion of BMEC... - Direct demonstration of ATP-dependent release of SecA from a translocating preprotein by surface plasmon resonanceJeanine de Keyzer
Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
J Biol Chem 278:29581-6. 2003..SecA binds tightly to the SecYEG.proOmpA complex and is released only upon ATP hydrolysis. The results provide direct evidence for a model in which SecA cycles at the SecYEG complex during translocation... - Folding and insertion of the outer membrane protein OmpA is assisted by the chaperone Skp and by lipopolysaccharidePaula V Bulieris
Fachbereich Biologie, Fach M 694, Universitat Konstanz, D 78457 Konstanz, Germany
J Biol Chem 278:9092-9. 2003We have studied the folding pathway of a beta-barrel membrane protein using outer membrane protein A (OmpA) of Escherichia coli as an example... - Escherichia coli outer membrane protein A adheres to human brain microvascular endothelial cellsSooan Shin
Division of Infectious Diseases, Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
Biochem Biophys Res Commun 330:1199-204. 2005..The outer membrane protein A (OmpA) assembles a beta-barrel structure having four surface-exposed loops in E. coli outer membrane... - Bacterial phage receptors, versatile tools for display of polypeptides on the cell surfaceH Etz
Antigen Discovery Group, Intercell Biomedizinische Forschungs und Entwicklungs AG, Rennweg 95B, 1030 Vienna, Austria
J Bacteriol 183:6924-35. 2001..In contrast, the receptors of the phages K3 and lambda, OmpA and LamB, accepted only insertions in their respective loop 4 of up to 40 amino acids containing the T7 tag... - Promiscuous substrate recognition in folding and assembly activities of the trigger factor chaperoneErik Martinez-Hackert
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
Cell 138:923-34. 2009..The structure of the complex reveals the molecular basis of substrate recognition by TF, indicates how TF could accelerate protein folding, and suggests a role for TF in the biogenesis of protein complexes... - OmpA influences Escherichia coli biofilm formation by repressing cellulose production through the CpxRA two-component systemQun Ma
Artie McFerrin Department of Chemical Engineering, Texas A and M University, College Station, TX 77843 3122, USA
Environ Microbiol 11:2735-46. 2009Previously we discovered that OmpA of Escherichia coli increases biofilm formation on polystyrene surfaces (González Barrios et al., Biotechnol Bioeng, 93:188-200, 2006a)... - Charged amino acids in a preprotein inhibit SecA-dependent protein translocationNico Nouwen
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands
J Mol Biol 386:1000-10. 2009..These results indicate that both clusters of positively and negatively charged amino acids are poor substrates for the Sec translocase and that this is reflected by their inability to stimulate the ATPase activity of SecA... - Changes in Escherichia coli outer membrane subproteome under environmental conditions inducing the viable but nonculturable stateAlicia Muela
Departamento de Inmunologia, Microbiologia y Parasitologia, Facultad de Ciencia y Tecnologia, Universidad del Pais Vasco, Bilbao, Spain
FEMS Microbiol Ecol 64:28-36. 2008..However, proteins exclusive to the VBNC state were not detected... - The trimeric periplasmic chaperone Skp of Escherichia coli forms 1:1 complexes with outer membrane proteins via hydrophobic and electrostatic interactionsJian Qu
Fachbereich Biologie, Universitat Konstanz, Universitatsstrasse 10, D 78464 Konstanz, Germany
J Mol Biol 374:91-105. 2007..We have examined the binding of Skp to various OMPs of different origin, size, and function. These were OmpA, OmpG, and YaeT (Omp85) from Escherichia coli, the translocator domain of the autotransporter NalP from Neisseria .. - Genes under positive selection in Escherichia coliLise Petersen
Bioinformatics Centre, University of Copenhagen, Copenhagen DK 2200, Denmark
Genome Res 17:1336-43. 2007..Based on structural evidence, we hypothesize that the selection acting on transposases is related to the genomic conflict between transposable elements and the host genome... - Sorting signal of Escherichia coli OmpA is modified by oligo-(R)-3-hydroxybutyrateMo Xian
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA
Biochim Biophys Acta 1768:2660-6. 2007Escherichia coli outer membrane protein A (OmpA) is a well-established model for the study of membrane assembly... - Adapted tolerance to benzalkonium chloride in Escherichia coli K-12 studied by transcriptome and proteome analysesErlend Bore
MATFORSK, Norwegian Food Research Institute, Osloveien 1, N 1430 As, Norway
Microbiology 153:935-46. 2007..The results revealed that BC treatment might result in superoxide stress in E. coli... - Amphipathic polymers: tools to fold integral membrane proteins to their active formCosmin L Pocanschi
Fachbereich Biologie, Fach M 694, Universitat Konstanz, D 78457 Konstanz, Germany
Biochemistry 45:13954-61. 2006..The feasibility of the approach is demonstrated using as models OmpA and FomA, two outer membrane proteins from the eubacteria Escherichia coli and Fusobacterium nucleatum, .. - Electrostatic couplings in OmpA ion-channel gating suggest a mechanism for pore openingHeedeok Hong
Department of Molecular Physiology and Biological Physics, University of Virginia Health System, PO Box 800736, Charlottesville, Virginia 22908 0736, USA
Nat Chem Biol 2:627-35. 2006..The gate of the OmpA channel is formed by the central Glu52-Arg138 salt bridge, which can open to form alternate ion pairs with Lys82 .. - The majority of Escherichia coli mRNAs undergo post-transcriptional modification in exponentially growing cellsBijoy K Mohanty
Department of Genetics, University of Georgia, Athens, GA 30602, USA
Nucleic Acids Res 34:5695-704. 2006..PCR analysis indicates that the extent of polyadenylation of individual full-length transcripts such as lpp and ompA varies significantly in wild-type cells. The data presented here demonstrates that polyadenylation in E... - Probing the adaptive response of Escherichia coli to extracellular Zn(II)Tara K Sigdel
Department of Chemistry and Biochemistry, Miami University, 112 Hughes Hall, Oxford, OH 45056, USA
Biometals 19:461-71. 2006..coli proteins, and the results are discussed in light of recent genomic profiling studies on the adaptive response of E. coli cells to stress by Zn(II) excess... - Interaction of E. coli outer-membrane protein A with sugars on the receptors of the brain microvascular endothelial cellsDeepshikha Datta
Materials and Process Simulation Center MC 139 74, California Institute of Technology, Pasadena, California 91125, USA
Proteins 50:213-21. 2003..Experimental studies have shown that outer-membrane protein A (OmpA) of E... - The influence of amino substitutions within the mature part of an Escherichia coli outer membrane protein (OmpA) on assembly of the polypeptide into its membraneM Klose
Max Planck Institut fur Biologie, Tubingen, Federal Republic of Germany
J Biol Chem 263:13297-302. 1988The membrane part of the 325-residue outer membrane protein OmpA of Escherichia coli encompasses residues 1-177... - OmpA protein of Escherichia coli outer membrane occurs in open and closed channel formsE Sugawara
Department of Molecular and Cell Biology, University of California, Berkeley 94720 3206
J Biol Chem 269:17981-7. 1994b>OmpA protein of Escherichia coli outer membrane can produce diffusion channels when reconstituted into proteoliposomes (Sugawara, E., and Nikaido, H. (1992) J. Biol. Chem. 267, 2507-2511)... - Kinetics of folding and membrane insertion of a beta-barrel membrane proteinT Surrey
Max Planck Institut fur Biologie, Abteilung Membranbiochemie, Tubingen, Federal Republic of Germany
J Biol Chem 270:28199-203. 1995We have studied the kinetics of folding and membrane insertion of the outer membrane protein OmpA of Escherichia coli. In the native structure, its membrane-inserted domain forms a beta-barrel... - Interaction of cAMP receptor protein with the ompA gene, a gene for a major outer membrane protein of Escherichia coliR N Movva
FEBS Lett 128:186-90. 1981 - Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12R Chen
Proc Natl Acad Sci U S A 77:4592-6. 1980The amino acid sequence of major outer membrane protein II (ompA protein) from Escherichia coli K-12 has been determined. The transmembrane polypeptide consists of 325 residues, resulting in a molecular weight of 35,159... - Resistance to apramycin in Escherichia coli isolated from animals: detection of a novel aminoglycoside-modifying enzymeR W Hedges
J Gen Microbiol 130:473-82. 1984..Synthesis of this enzyme was specified by a chromosomal gene located near pyrD at about 20 min on the map of the E. coli K12 chromosome... - Characterisation of the promoters for the ompA gene which encodes a major outer membrane protein of Escherichia coliS T Cole
Mol Gen Genet 188:472-9. 1982The regulatory region of the ompA gene from Escherichia coli has been characterized by biochemical and genetic approaches... - Resolving the native conformation of Escherichia coli OmpAAlexander Negoda
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA
FEBS J 277:4427-37. 2010The native conformation of the 325-residue outer membrane protein A (OmpA) of Escherichia coli has been a matter of contention. A narrow-pore, two-domain structure has vied with a large-pore, single-domain structure... - Internal deletions in the gene for an Escherichia coli outer membrane protein define an area possibly important for recognition of the outer membrane by this polypeptideM Klose
Max Planck Institut fur Biologie, Tubingen, Federal Republic of Germany
J Biol Chem 263:13291-6. 1988A series of overlapping deletions has been constructed in the ompA gene which encodes the 325-residue Escherichia coli outer membrane protein OmpA... - Decay of ompA mRNA and processing of 9S RNA are immediately affected by shifts in growth rate, but in opposite mannersD Georgellis
Karolinska Institute, Department of Bacteriology, Stockholm, Sweden
J Bacteriol 174:5382-90. 1992..coli in continuous cultures at various growth rates, we provide definitive evidence that the stability of the ompA mRNA is growth rate dependent... - A 5'-terminal stem-loop structure can stabilize mRNA in Escherichia coliS A Emory
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
Genes Dev 6:135-48. 1992The 5'-untranslated region of the long-lived Escherichia coli ompA transcript functions as an mRNA stabilizer capable of prolonging the lifetime in E. coli of a number of heterologous messages to which it is fused... - Major proteins of the Escherichia coli outer cell envelope membrane. Interaction of protein II with lipopolysaccharideM Schweizer
Eur J Biochem 82:211-7. 1978 - Major proteins of the Escherichia coli outer cell envelope membrane as bacteriophage receptorsD B Datta
J Bacteriol 131:821-9. 1977..In addition, the association of proteins Ia and Ib with the murein layer of the cell envelope makes this pair trans-membrane proteins... - Prediction of transmembrane beta-strands from hydrophobic characteristics of proteinsM M Gromiha
Department of Physics, Bharathidasan University, Tiruchirapalli, Tamil Nadu, India
Int J Pept Protein Res 42:420-31. 1993..The predicted beta-structure contents in OmpA, porin from E. coli and maltoporin compared with the Raman spectroscopic results at 95% level... - Hfq (HF1) stimulates ompA mRNA decay by interfering with ribosome bindingO Vytvytska
Institute of Microbiology and Genetics, Vienna Biocenter, 1030 Vienna, Austria
Genes Dev 14:1109-18. 2000..The Escherichia coli ompA mRNA has served as one of the paradigms for regulated mRNA decay in prokaryotes... - Polyphosphate kinase is a component of the Escherichia coli RNA degradosomeE Blum
Nuffield Department of Clinical Biochemistry, Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, UK
Mol Microbiol 26:387-98. 1997..An E. coli strain deleted for the ppk gene showed increased stability of the ompA mRNA. Purified His-tagged PPK was shown to bind RNA, and RNA binding was prevented by hydrolysable ATP... - Structural and functional roles of the surface-exposed loops of the beta-barrel membrane protein OmpA from Escherichia coliR Koebnik
Max Planck Institut fur Biologie, Abteilung Mikrobiologie, D 72076 Tubingen, Germany
J Bacteriol 181:3688-94. 1999The N-terminal domain of the OmpA protein from Escherichia coli, consisting of 170 amino acid residues, is embedded in the outer membrane, in the form of an antiparallel beta-barrel whose eight transmembrane beta-strands are connected by .. - TonB interacts with nonreceptor proteins in the outer membrane of Escherichia coliPenelope I Higgs
School of Molecular Biosciences, Washington State University, Pullman, Washington 99164 4233, USA
J Bacteriol 184:1640-8. 2002..Subsequently, two additional contacts of TonB with the outer membrane proteins Lpp and, putatively, OmpA were identified by in vivo cross-linking... - Structure of outer membrane protein A transmembrane domain by NMR spectroscopyA Arora
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908 0736, USA
Nat Struct Biol 8:334-8. 2001..Moreover, data from NMR dynamic experiments reveal a gradient of conformational flexibility in the structure that may contribute to the membrane channel function of this protein... - Host factor I, Hfq, binds to Escherichia coli ompA mRNA in a growth rate-dependent fashion and regulates its stabilityO Vytvytska
Institute of Microbiology and Genetics, Vienna Biocenter, University of Vienna, Dr Bohr Gasse 9, A 1030 Vienna, Austria
Proc Natl Acad Sci U S A 95:14118-23. 1998The stability of the ompA mRNA depends on the bacterial growth rate. The 5' untranslated region is the stability determinant of this transcript and the target of the endoribonuclease, RNase E, the key player of mRNA degradation... - Outer membrane protein A of Escherichia coli inserts and folds into lipid bilayers by a concerted mechanismJ H Kleinschmidt
Department of Molecular Physiology and Biological Physics, Center for Structural Biology, University of Virginia Health Sciences Center, Charlottesville 22906 0011, USA
Biochemistry 38:5006-16. 1999Unfolded outer membrane protein A (OmpA) of Escherichia coli spontaneously inserts and refolds into lipid bilayers upon dilution of denaturing urea... - Proteomics on full-length membrane proteins using mass spectrometryJ le Coutre
Howard Hughes Medical Institute, Departments of Physiology and of Microbiology and Molecular Genetics, Molecular Biology Institute, University of California, Los Angeles 90095, USA
Biochemistry 39:4237-42. 2000..Finally, using in-line LC-MS, unknown proteins can be identified from solubilized Escherichia coli membranes without prior purification... - Basic amino acids in a distinct subset of signal peptides promote interaction with the signal recognition particleJanine H Peterson
Genetics and Biochemistry Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 0538, USA
J Biol Chem 278:46155-62. 2003..acids in a signal peptide influence the targeting of two Escherichia coli proteins, maltose binding protein and OmpA. Whereas both proteins are normally targeted to the inner membrane by SecB, we found that replacement of their .. - Molecular considerations in the evolution of bacterial genesJ G Lawrence
Department of Genetics, Washington University School of Medicine, St Louis, MO 63110
J Mol Evol 33:241-50. 1991..rates at the loci encoding glyceraldehyde-3-phosphate dehydrogenase (gap) and outer membrane protein 3A (ompA) were examined in 12 species of enteric bacteria... - Major outer membrane protein d of Escherichia coli K12. Purification and in vitro activity of bacteriophages k3 and f-pilus mediated conjugationL van Alphen
FEBS Lett 75:285-90. 1977 - SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coliHolger Thie
Technische Universitat Braunschweig, Institut für Biochemie und Biotechnologie, Abteilung Biotechnologie, Spielmannstr 7, 38106 Braunschweig, Germany
N Biotechnol 25:49-54. 2008..Four leader peptides using the Sec pathway (PelB, OmpA, PhoA and pIII) and three using the SRP pathway (DsbA, TorT and TolB) were compared... - Controlling membrane permeability with bacterial porins: application to encapsulated enzymesM Winterhalter
Institut de Pharmacologie et Biologie Structurale, UMR, CNRS 5089, 205, rte de Narbonne, F 31077 Toulouse, France
Talanta 55:965-71. 2001..Another application of this kind of stabilisation is in the field of single enzyme activity recording... - The cavity-chaperone Skp protects its substrate from aggregation but allows independent folding of substrate domainsTroy A Walton
Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA
Proc Natl Acad Sci U S A 106:1772-7. 2009..The 17-kDa protein (Skp) is a periplasmic chaperone that assists the folding and insertion of many OMPs, including OmpA, a model OMP with a membrane embedded beta-barrel domain and a periplasmic alphabeta domain... - Ligand crowding at a nascent signal sequenceGottfried Eisner
Institut fur Biochemie und Molekularbiologie, Hermann Herder Strasse 7, D 79104 Freiburg, Germany
J Cell Biol 163:35-44. 2003..coli secretory protein gradually becomes sequestered by TF. Although TF thereby might control the accessibility of pOmpA's signal sequence to Ffh and SecA, it does not influence interaction of pOmpA with SecB... - Export chaperone SecB uses one surface of interaction for diverse unfolded polypeptide ligandsAngela A Lilly
Department of Biochemistry, University of Missouri, Columbia, Missouri 65211, USA
Protein Sci 18:1860-8. 2009..We examined SecB in complex with the unfolded precursor form of outer membrane protein OmpA as well as with a truncated version of OmpA that includes the transmembrane domain and lacks both the signal .. - NMR study of a membrane protein in detergent-free aqueous solutionManuela Zoonens
Unite Mixte de Recherche 7099, Centre National de la Recherche Scientifique CNRS Université Paris 7, Institut de Biologie Physico Chimique CNRS FRC 550, 11 rue Pierre et Marie Curie, F 75005 Paris, France
Proc Natl Acad Sci U S A 102:8893-8. 2005..The potential of this approach to MP studies by solution NMR is discussed... - The periplasmic chaperone Skp facilitates targeting, insertion, and folding of OmpA into lipid membranes with a negative membrane surface potentialGeetika J Patel
Fachbereich Biologie, Universitat Konstanz, Germany
Biochemistry 48:10235-45. 2009..Here we compare spontaneous folding of outer membrane protein A (OmpA) of Escherichia coli from its urea-unfolded form and from the complex with its periplasmic chaperone Skp into lipid .. - Molecular dynamics simulations of a membrane protein-micelle complex in vacuoRosmarie Friemann
Department of Cell and Molecular Biology, Uppsala University, Box 596, SE 751 24 Uppsala, Sweden
J Am Chem Soc 131:16606-7. 2009.... - Sigma E controls biogenesis of the antisense RNA MicAKlas I Udekwu
Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, S 75124 Uppsala, Sweden
Nucleic Acids Res 35:1279-88. 2007..work by us and others showed that the antisense RNA MicA downregulates the synthesis of the outer membrane protein OmpA upon entry into stationary phase... - OmpA of a septicemic Escherichia coli O78--secretion and convergent evolutionUri Gophna
Department of Molecular Microbiology and Biotechnology, The George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Int J Med Microbiol 294:373-81. 2004b>OmpA is an important constituent of the outer membrane of Gram-negative bacteria. OmpA is involved in a variety of host-bacteria interactions, including crossing of the blood-brain barrier by E... - Serum amyloid A protein binds to outer membrane protein A of gram-negative bacteriaRanjeeta Hari-Dass
Immunology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
J Biol Chem 280:18562-7. 2005..by matrix-assisted laser desorption ionization time-of-flight mass spectrometry to be outer membrane protein A (OmpA). OmpA-deficient E. coli did not bind SAA, and following purification of OmpA the protein retained binding activity... - Orientation of beta-barrel proteins OmpA and FhuA in lipid membranes. Chain length dependence from infrared dichroismMuthu Ramakrishnan
Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
Biochemistry 44:3515-23. 2005The outer-membrane proteins OmpA and FhuA of Escherichia coli are monomeric beta-barrels of widely differing size... - Stepwise movement of preproteins in the process of translocation across the cytoplasmic membrane of Escherichia coliK Uchida
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
J Biol Chem 270:30862-8. 1995..These results can be best interpreted as that the translocation of preproteins through the secretory machinery takes place in every 30 amino acid residues and that SecA is responsible for the stepwise movement... - Synonymous codon selection controls in vivo turnover and amount of mRNA in Escherichia coli bla and ompA genesA Deana
Laboratoire Structure et Dynamique du Génome, Centre de Genetique Moleculaire, Centre National de la Recherch Scientifique, Gif sur Yvette, France
J Bacteriol 178:2718-20. 1996A number of silent codon changes were made in two Escherichia coli genes. For the ompA gene, the replacement of seven consecutive frequently used codons with synonymous infrequently used codons reduced the ompA mRNA level and its half-.. - Secondary and tertiary structure formation of the beta-barrel membrane protein OmpA is synchronized and depends on membrane thicknessJörg H Kleinschmidt
Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0736, USA
J Mol Biol 324:319-30. 2002..insertion and folding of a beta-barrel membrane protein has been studied using the outer membrane protein A (OmpA) as an example... - Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresisM P Molloy
Australian Proteome Analysis Facility, School of Biological Sciences, Macquarie University, Sydney, NSW, Australia
Electrophoresis 19:837-44. 1998.... - Outer membrane protein A of Escherichia coli forms temperature-sensitive channels in planar lipid bilayersE Zakharian
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA
FEBS Lett 555:229-35. 2003The temperature dependence of single-channel conductance and open probability for outer membrane protein A (OmpA) of Escherichia coli were examined in planar lipid bilayers... - Growth of Escherichia coli MG1655 on LB medium: monitoring utilization of amino acids, peptides, and nucleotides with transcriptional microarraysMark V Baev
Integrated Genomics Inc, Chicago, IL 60612, USA
Appl Microbiol Biotechnol 71:317-22. 2006..glnALG, glnK-amtB, dppABCDF, and oppABCDF operons as well as the genes coding for outer membrane proteins, porins OmpA and OmpC, and proteases OmpP and OmpT... - Folding kinetics of the outer membrane proteins OmpA and FomA into phospholipid bilayersJörg H Kleinschmidt
Fachbereich Biologie, Fach M 694, Universitat Konstanz, Germany
Chem Phys Lipids 141:30-47. 2006..mechanism of outer membrane proteins (OMPs) of Gram-negative bacteria into lipid bilayers has been studied using OmpA of E. coli and FomA of F. nucleatum as examples... - Mutual regulation of Crl and Fur in Escherichia coli W3110Cécile Lelong
Laboratoire Adaptation et Pathogenie des Microorganismes, Institut Jean Roget, Domaine de la Merci, Universite Joseph Fourier, BP 170, 38042 Grenoble Cedex 9, France
Mol Cell Proteomics 6:660-8. 2007..Crl modulated the affinity of Fur at the fur promoter but not at the crl promoter. The triad RpoS-Crl-Fur may thus represent the centerpiece of a global regulatory system of response to different stresses... - Increasing the accuracy of solution NMR structures of membrane proteins by application of residual dipolar couplings. High-resolution structure of outer membrane protein ATomasz Cierpicki
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
J Am Chem Soc 128:6947-51. 2006..Here we demonstrate that significant improvement in the structure accuracy of the membrane protein OmpA can be achieved by refinement with residual dipolar couplings (RDCs)... - Bacteriophage receptor area of outer membrane protein OmpA of Escherichia coli K-12R Morona
J Bacteriol 164:539-43. 1985A number of T-even-like bacteriophages use the outer membrane protein OmpA of Escherichia coli as a receptor... - Chaperonins govern growth of Escherichia coli at low temperaturesManuel Ferrer
Nat Biotechnol 21:1266-7. 2003 - Escherichia coli K-12 outer membrane protein (OmpA) as a bacteriophage receptor: analysis of mutant genes expressing altered proteinsR Morona
J Bacteriol 159:570-8. 1984The outer membrane protein OmpA of Escherichia coli K-12 serves as a receptor for a number of T-even-like phages. We have isolated a series of ompA mutants which are resistant to such phages but which still produce the OmpA protein... - Beta-barrel proteins that reside in the Escherichia coli outer membrane in vivo demonstrate varied folding behavior in vitroNancy K Burgess
T C Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218, USA
J Biol Chem 283:26748-58. 2008..We cloned, expressed, and folded nine OMPs: outer membrane protein X (OmpX), OmpW, OmpA, the crcA gene product (PagP), OmpT, outer membrane phospholipase A (OmpLa), the fadl gene product (FadL), the yaet .. - Pore-forming activity of OmpA protein of Escherichia coliE Sugawara
Department of Molecular and Cell Biology, University of California, Berkeley 94720
J Biol Chem 267:2507-11. 1992Escherichia coli outer membrane protein OmpA was purified to homogeneity, as a monomer, from a K12 derivative deficient in both OmpF and OmpC porins... - Structure of the outer membrane protein A transmembrane domainA Pautsch
Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Freiburg im Breisgau, Germany
Nat Struct Biol 5:1013-7. 1998The outer membrane protein A of Escherichia coli (OmpA) is an intensely studied example in the field of membrane protein folding... - RNase R affects gene expression in stationary phase: regulation of ompAJosé Marques Andrade
Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Apartado 127, 2781 901 Oeiras, Portugal
Mol Microbiol 60:219-28. 2006..demonstrate that RNase R is induced in stationary phase and is involved in the post-transcriptional regulation of ompA mRNA. This work is the first report of RNase R activity on a full length mRNA... - Translational control and target recognition by Escherichia coli small RNAs in vivoJohannes H Urban
Max Planck Institute for Infection Biology, RNA Biology Group, Chariteplatz 1, 10117 Berlin, Germany
Nucleic Acids Res 35:1018-37. 2007..g. despite the fact that ompA fusion mRNA decay could no longer be promoted by MicA. This is the first study in which multiple well-defined E...