Gene Symbol: NUP60
- A nuclear envelope protein linking nuclear pore basket assembly, SUMO protease regulation, and mRNA surveillanceAlaron Lewis
Departments of Cell Biology, Yale University, New Haven, CT 06520, USA
J Cell Biol 178:813-27. 2007..In esc1Delta cells, Ulp1 and nuclear basket components Nup60 and Mlp1 no longer distribute broadly around the nuclear periphery, but co-localize in a small number of dense-..
- Minimal nuclear pore complexes define FG repeat domains essential for transportLisa A Strawn
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, 3120A MRBIII, 465 21st Avenue South, Nashville, TN 37232 8240, USA
Nat Cell Biol 6:197-206. 2004..Significantly, symmetric deletions caused mild reductions in Kap95-Kap60-mediated import rates, but virtually abolished Kap104 import. These results suggest the existence of multiple translocation pathways...
- A gradient of affinity for the karyopherin Kap95p along the yeast nuclear pore complexBrook Pyhtila
Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
J Biol Chem 278:42699-709. 2003..We conclude that a high affinity binding site for Kap95p at the nuclear basket increases the translocation efficiency of Kap95p import complexes across the NPC...
- The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear poresTamas Fischer
Biochemie Zentrum Heidelberg BZH, Im Neuenheimer Feld 328, Germany
EMBO J 21:5843-52. 2002..Significantly, mutations in Sac3p or Thp1p lead to strong mRNA export defects. Taken together, our data suggest that the novel Sac3p-Thp1p complex functions by docking the mRNP to specific nucleoporins at the nuclear entrance of the NPC...
- Nup2p dynamically associates with the distal regions of the yeast nuclear pore complexD J Dilworth
Institute for Systems Biology, Seattle, Washington, 98105, USA
J Cell Biol 153:1465-78. 2001..On the nucleoplasmic face, where the Ran--GTP levels are predicted to be high, Nup2p binds to Nup60p. Deletion of NUP60 renders Nup2p nucleoplasmic and compromises Nup2p-mediated recycling of Kap60p/Srp1p...
- Nuclear architecture and spatial positioning help establish transcriptional states of telomeres in yeastFrank Feuerbach
Laboratoire de Biologie Cellulaire du Noyau, CNRS URA1773, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
Nat Cell Biol 4:214-21. 2002..Our data support a model in which domains of enriched transcriptional regulators allow genes to determine transcriptional states by spatial positioning...
- The nucleoporin Nup60p functions as a Gsp1p-GTP-sensitive tether for Nup2p at the nuclear pore complexD Denning
Cancer Biology Program, Stanford Medical School, Stanford University, CA 94305, USA
J Cell Biol 154:937-50. 2001..The results suggest a dynamic interaction, controlled by the nucleoplasmic concentration of Gsp1p-GTP, between Nup60p and Nup2p at the NPC...
- Prevalent positive epistasis in Escherichia coli and Saccharomyces cerevisiae metabolic networksXionglei He
Department of Ecology and Evolutionary Biology, Ann Arbor, Michigan, USA
Nat Genet 42:272-6. 2010..We offer mechanistic explanations of these findings and experimentally validate them for 61 S. cerevisiae gene pairs...
- An endoribonuclease functionally linked to perinuclear mRNP quality control associates with the nuclear pore complexesMichal Skruzny
Biochemie Zentrum der Universität Heidelberg BZH, Heidelberg, Germany
PLoS Biol 7:e8. 2009..Swt1 and the THO/TREX and TREX-2 complexes, and with components of the perinuclear mRNP surveillance system, Mlp1, Nup60, and Esc1...
- General, rapid, and transcription-dependent fragmentation of nucleolar antigens in S. cerevisiae mRNA export mutantsRune Thomsen
Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, University of Aarhus, DK 8000 Aarhus C, Denmark
RNA 14:706-16. 2008..Consistent with a causal role of excess nuclear mRNA, nucleolar morphology in an mRNA export mutant environment remains intact when transcription by RNA polymerase II is inhibited...
- Arx1 is a nuclear export receptor for the 60S ribosomal subunit in yeastNai Jung Hung
Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Mol Biol Cell 19:735-44. 2008..These results show that Arx1 can directly bridge the interaction between the pre-60S particle and the NPC and thus is a third export receptor for the 60S subunit in yeast...
- Discovering novel interactions at the nuclear pore complex using bead halo: a rapid method for detecting molecular interactions of high and low affinity at equilibriumSamir S Patel
Department of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, CA 95064, USA
Mol Cell Proteomics 7:121-31. 2008..Finally, Bead Halo detected binding of the nups Gle1, Nup60, and Nsp1 to phospholipid bilayers...
- Lumenal interactions in nuclear pore complex assembly and stabilityWilliam T Yewdell
Howard Hughes Medical Institute, New York, NY 10065, USA
Mol Biol Cell 22:1375-88. 2011..These findings support a role for Heh1p in the assembly or stability of the NPC, potentially through the formation of a lumenal bridge with Pom152p...
- Nuclear mRNA export requires specific FG nucleoporins for translocation through the nuclear pore complexLaura J Terry
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
J Cell Biol 178:1121-32. 2007..These results pinpoint distinct steps in the mRNA export mechanism that regulate NPC translocation efficiency...
- Topoisomerase I-dependent viability loss in saccharomyces cerevisiae mutants defective in both SUMO conjugation and DNA repairXiaole L Chen
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Genetics 177:17-30. 2007..Sumoylation is also required for growth of top1Delta cells. These results suggest that the SUMO pathway has a complex effect on genome stability that involves several mechanistically distinct processes...
- Proteome-wide identification of in vivo targets of DNA damage checkpoint kinasesMarcus B Smolka
Ludwig Institute for Cancer Research, La Jolla, CA 92093 0653, USA
Proc Natl Acad Sci U S A 104:10364-9. 2007..In addition to several known targets, 50 previously undescribed targets of the DNA damage checkpoint were identified, suggesting that a wide range of cellular processes is likely regulated by Mec1, Tel1, and Rad53...
- Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processesBenoit Palancade
Institut Curie, Centre de Recherche, and Unité Mixte de Recherche 144 Centre National de la Recherche Scientifique, F 75248 Paris, France
Mol Biol Cell 18:2912-23. 2007..Here, we show that double-strand break (DSB) appearance is linked to a shared function of the Nup84 and the Nup60/Mlp1-2 complexes...
- The role of karyopherins in the regulated sumoylation of septinsTaras Makhnevych
Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
J Cell Biol 177:39-49. 2007..We present a model in which Ulp1p is maintained at the NPC during interphase and transiently interacts with the septin ring during mitosis...
- Interactions between Mad1p and the nuclear transport machinery in the yeast Saccharomyces cerevisiaeRobert J Scott
Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada, T6G 2H7
Mol Biol Cell 16:4362-74. 2005..Our further analysis also showed that only the C terminus of Mad1p is required for SAC function and that the NPC, through Nup53p, may act to regulate the duration of the SAC response...
- Nuclear pores and nuclear assemblyS K Vasu
Section of Cell and Developmental Biology, Division of Biology, University of California San Diego, La Jolla, CA 92093 0347, USA
Curr Opin Cell Biol 13:363-75. 2001....