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Genomes and Genes | NFI1SummaryGene Symbol: NFI1 Description: Nfi1p Alias: SIZ2 Species: Top Publications
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A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organizationXiaolan Zhao
Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Proc Natl Acad Sci U S A 102:4777-82. 2005..We propose that Mms21 sumoylates proteins involved in these diverse processes and that the other members of the complex, particularly Smc5/6, facilitate proper substrate sumoylation by localizing Mms21 to specific chromosomal regions...- Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongationBen Montpetit
Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
J Cell Biol 174:653-63. 2006..These data suggest that sumoylation of Ndc10 and other kinetochore proteins play a critical role during the mitotic process... - Topoisomerase I-dependent viability loss in saccharomyces cerevisiae mutants defective in both SUMO conjugation and DNA repairXiaole L Chen
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Genetics 177:17-30. 2007Siz1 and Siz2/Nfi1 are the two Siz/PIAS SUMO E3 ligases in Saccharomyces cerevisiae. Here we show that siz1Delta siz2Delta mutants fail to grow in the absence of the homologous recombination pathway or the Fen1 ortholog RAD27... - Stimulation of in vitro sumoylation by Slx5-Slx8: evidence for a functional interaction with the SUMO pathwayTatsuya Ii
Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854, USA
DNA Repair (Amst) 6:1679-91. 2007..Interestingly, a functional RING-finger domain is not required for this stimulation in vitro. These biochemical data demonstrate for the first time that the Slx5 and Slx8 complex is capable of interacting directly with the SUMO pathway... - Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in vitroYoshimitsu Takahashi
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033
J Biochem 133:415-22. 2003..We demonstrate here that the second PIAS-type Nfi1/Siz2 is also a SUMO ligase... - SIZ1/SIZ2 control of chromosome transmission fidelity is mediated by the sumoylation of topoisomerase IIYoshimitsu Takahashi
National Institutes of Health, National Institute of Child Health and Human Development, Laboratory of Gene Regulation and Development, Bethesda, Maryland 20892, USA
Genetics 172:783-94. 2006..By combining mutations in the TOP2 sumoylation sites and the SIZ1 and SIZ2 genes we demonstrate that the minichromosome segregation defect and dicentric minichromosome stabilization, both .. - Misregulation of 2 microm circle copy number in a SUMO pathway mutantXiaole L Chen
Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, 233 South 10th St, BLSB 231, Philadelphia, PA 19107, USA
Mol Cell Biol 25:4311-20. 2005..However, yeast mutants lacking the SUMO ligases Siz1 and Siz2/Nfi1 are viable, even though they show dramatically reduced levels of SUMO conjugation... - Saccharomyces cerevisiae SMT4 encodes an evolutionarily conserved protease with a role in chromosome condensation regulationA V Strunnikov
National Institute of Child Health and Human Development, National Library of Medicine, National Institutes of Health, Bethesda, MD 20892, USA
Genetics 158:95-107. 2001..The SIZ1 gene disruption is synthetically lethal with the SIZ2 deletion. We propose that SMT4, SIZ1, and SIZ2 are involved in a novel pathway of chromosome maintenance. - Deficient SUMO attachment to Flp recombinase leads to homologous recombination-dependent hyperamplification of the yeast 2 microm circle plasmidLing Xiong
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
Mol Biol Cell 20:1241-51. 2009..This work also illustrates the importance of using cir(o) strains when studying mutants that affect the yeast SUMO pathway, to avoid confusing direct functions of the SUMO pathway with secondary effects of 2 microm amplification... - Cooperation of sumoylated chromosomal proteins in rDNA maintenanceYoshimitsu Takahashi
Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, NIH, Bethesda, MD, USA
PLoS Genet 4:e1000215. 2008..In addition, binding of cohesin and condensin to rDNA is altered in the mms21-CH E3-deficient mutant... - Quality control of a transcriptional regulator by SUMO-targeted degradationZheng Wang
Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
Mol Cell Biol 29:1694-706. 2009..These results therefore demonstrate that Mot1 is an in vivo STUbL target in yeast and suggest a role for SUMO-targeted degradation in protein quality control... - The Saccharomyces cerevisiae Esc2 and Smc5-6 proteins promote sister chromatid junction-mediated intra-S repairJulie Sollier
IFOM, the FIRC Institute for Molecular Oncology Foundation, IFOM IEO Campus, 20139 Milan, Italy
Mol Biol Cell 20:1671-82. 2009.... - Sumoylation and the structural maintenance of chromosomes (Smc) 5/6 complex slow senescence through recombination intermediate resolutionAlejandro Chavez
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 285:11922-30. 2010..Further, we provide evidence that Mms21 and Smc5/6 prevent aberrant recombination between sister telomeres and also globally facilitate resolution of sister chromatid HR intermediates... - The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeastHelder C Ferreira
Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
Nat Cell Biol 13:867-74. 2011..We find that the PIAS-like SUMO E3 ligase Siz2 sumoylates both Yku70/80 and Sir4 in vivo and promotes telomere anchoring to the nuclear envelope... - SUMOylation regulates telomere length homeostasis by targeting Cdc13Lisa E Hang
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
Nat Struct Mol Biol 18:920-6. 2011.... - Elg1, the major subunit of an alternative RFC complex, interacts with SUMO-processing proteinsOren Parnas
Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel
Cell Cycle 10:2894-903. 2011..between the N terminus of Elg1 and Slx5 is mediated by poly-SUMO chains but not by PCNA modifications, and requires Siz2, but not Siz1, activity... - The spindle positioning protein Kar9p interacts with the sumoylation machinery in Saccharomyces cerevisiaeNida Meednu
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
Genetics 180:2033-55. 2008..We propose a model in which sumoylation regulates spindle positioning by restricting Kar9p to one SPB. These findings raise the possibility that sumoylation could regulate other microtubule-dependent processes... - SUMO mediates interaction of Ebp2p, the yeast homolog of Epstein-Barr virus nuclear antigen 1-binding protein 2, with a RING finger protein Ris1pChiharu Shirai
Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Kagamiyama, Higashi Hiroshima, Japan
Biosci Biotechnol Biochem 72:1881-6. 2008..A SUMO-binding motif of Ris1p was required for interaction with Ebp2p. These results suggest that SUMO mediates the interaction between Ebp2p and SUMO related proteins and that Ebp2p switches its interaction partners via sumoylation... - Smc5-Smc6 complex suppresses gross chromosomal rearrangements mediated by break-induced replicationsJi Young Hwang
Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, 49 Convent Drive, Bethesda, MD 20892 USA
DNA Repair (Amst) 7:1426-36. 2008.... - An E3-like factor that promotes SUMO conjugation to the yeast septinsE S Johnson
Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107, USA
Cell 106:735-44. 2001..cerevisiae septins in vivo and strongly stimulates septin sumoylation in vitro. Siz1 and the related protein Siz2 promote SUMO conjugation to different substrates at different stages of the cell cycle and, together, are required .. - The SUMO ubiquitin-protein isopeptide ligase family member Miz1/PIASxbeta /Siz2 is a transcriptional cofactor for TFII-IMaria Isabel Tussie-Luna
Department of Pathology, Programs in Immunology and Genetics, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
J Biol Chem 277:43185-93. 2002..and mouse cDNA libraries in parallel, we have identified the RING-like zinc finger containing Miz1/PIASxbeta/Siz2, which is a ubiquitin-protein isopeptide ligase in the SUMO pathway, as the potential nuclear cofactor that .. - Proteins interacting with Lin 1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiaeAgnieszka Bialkowska
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Poland
Yeast 19:1323-33. 2002..second round of two-hybrid assay with Lin1p as the bait resulted in the identification of six proteins: Prp8, Slx5, Siz2, Wss1, Rfc1 and YIL149w... - Genetic analysis connects SLX5 and SLX8 to the SUMO pathway in Saccharomyces cerevisiaeZheng Wang
Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
Genetics 172:1499-509. 2006.... - The jmjN and jmjC domains of the yeast zinc finger protein Gis1 interact with 19 proteins involved in transcription, sumoylation and DNA repairSusanna Tronnersjö
Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, P O Box 7080, 75007, Uppsala, Sweden
Mol Genet Genomics 277:57-70. 2007..helicase Sgs1, the silencing factors Esc1 and Sir4, the URI-type prefoldin Bud27 and the PIAS type SUMO ligase Nfi1/Siz2. Extensive interaction cross dependencies further suggest that the proteins form a larger complex... - Multiple domains in Siz SUMO ligases contribute to substrate selectivityAlison Reindle
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
J Cell Sci 119:4749-57. 2006Saccharomyces cerevisiae contains two Siz/PIAS SUMO E3 ligases, Siz1 and Siz2/Nfi1, and one other known ligase, Mms21... - Ecm11 protein of yeast Saccharomyces cerevisiae is regulated by sumoylation during meiosisApolonija Bedina Zavec
Laboratory for Biosynthesis and Biotransformation, National Institute of Chemistry, Hajdrihova, Ljubljana, Slovenia
FEMS Yeast Res 8:64-70. 2008..It was shown that Ecm11 interacts with Siz2 SUMO ligase in a two-hybrid system; although Siz2 is not essential for the Ecm11 sumoylation. - Activation of the Slx5-Slx8 ubiquitin ligase by poly-small ubiquitin-like modifier conjugatesJanet R Mullen
Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854, USA
J Biol Chem 283:19912-21. 2008..To determine the specificity of Slx5-Slx8, we assayed its Ub ligase activity using sumoylated Siz2 as an in vitro substrate... - A role for SUMO in nucleotide excision repairHannah R Silver
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
DNA Repair (Amst) 10:1243-51. 2011The two Siz/PIAS SUMO E3 ligases Siz1 and Siz2 are responsible for the vast majority of sumoylation in Saccharomyces cerevisiae. We found that siz1? siz2? mutants are sensitive to ultra-violet (UV) light...