pknB

Summary

Gene Symbol: pknB
Description: serine/threonine-protein kinase PknB
Species: Mycobacterium tuberculosis H37Rv
Products:     pknB

Top Publications

  1. Young T, Delagoutte B, Endrizzi J, Falick A, Alber T. Structure of Mycobacterium tuberculosis PknB supports a universal activation mechanism for Ser/Thr protein kinases. Nat Struct Biol. 2003;10:168-74 pubmed
    ..Here we characterize PknB, a transmembrane signaling kinase from Mycobacterium tuberculosis...
  2. Parikh A, Verma S, Khan S, Prakash B, Nandicoori V. PknB-mediated phosphorylation of a novel substrate, N-acetylglucosamine-1-phosphate uridyltransferase, modulates its acetyltransferase activity. J Mol Biol. 2009;386:451-64 pubmed publisher
    ..and biochemical studies have shown that Mycobacterium tuberculosis serine/threonine protein kinases PknA and PknB play a role in modulating cell shape and possibly cell division...
  3. Boitel B, Ortiz Lombardia M, Duran R, Pompeo F, Cole S, Cerveñansky C, et al. PknB kinase activity is regulated by phosphorylation in two Thr residues and dephosphorylation by PstP, the cognate phospho-Ser/Thr phosphatase, in Mycobacterium tuberculosis. Mol Microbiol. 2003;49:1493-508 pubmed
    ..Here we focus on the catalytic domains of two trans-membrane enzymes, the Ser/Thr protein kinase PknB and the protein phosphatase PstP from Mycobacterium tuberculosis...
  4. Mieczkowski C, Iavarone A, Alber T. Auto-activation mechanism of the Mycobacterium tuberculosis PknB receptor Ser/Thr kinase. EMBO J. 2008;27:3186-97 pubmed publisher
    ..determined the crystal structure of a mutant of the Ser/Thr kinase domain (KD) of the mycobacterial sensor kinase PknB in complex with an ATP competitive inhibitor and discovered features consistent with an activation complex...
  5. Prisic S, Dankwa S, Schwartz D, Chou M, Locasale J, Kang C, et al. Extensive phosphorylation with overlapping specificity by Mycobacterium tuberculosis serine/threonine protein kinases. Proc Natl Acad Sci U S A. 2010;107:7521-6 pubmed publisher
    ..tuberculosis and create a resource for understanding how specific phosphorylation events modulate protein activity. The results further provide the potential to predict likely cognate STPKs for newly identified phosphoproteins. ..
  6. Chawla Y, Upadhyay S, Khan S, Nagarajan S, Forti F, Nandicoori V. Protein kinase B (PknB) of Mycobacterium tuberculosis is essential for growth of the pathogen in vitro as well as for survival within the host. J Biol Chem. 2014;289:13858-75 pubmed publisher
    The Mycobacterium tuberculosis protein kinase B (PknB) comprises an intracellular kinase domain, connected through a transmembrane domain to an extracellular region that contains four PASTA domains...
  7. Kang C, Abbott D, Park S, Dascher C, Cantley L, Husson R. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005;19:1692-704 pubmed
    The Mycobacterium tuberculosis genome contains 11 serine/threonine kinase genes including two, pknA and pknB, that are part of an operon encoding genes involved in cell shape control and cell wall synthesis...
  8. Mir M, Asong J, Li X, Cardot J, Boons G, Husson R. The extracytoplasmic domain of the Mycobacterium tuberculosis Ser/Thr kinase PknB binds specific muropeptides and is required for PknB localization. PLoS Pathog. 2011;7:e1002182 pubmed publisher
    The Mycobacterium tuberculosis Ser/Thr kinase PknB has been implicated in the regulation of cell growth and morphology in this organism...
  9. Villarino A, Duran R, Wehenkel A, Fernandez P, England P, Brodin P, et al. Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA domain-containing protein, through activation loop-mediated interactions. J Mol Biol. 2005;350:953-63 pubmed
    ..of GarA (Rv1827), a Forkhead-associated (FHA) domain-containing protein, as a putative physiological substrate of PknB, an essential Ser/Thr protein kinase from Mycobacterium tuberculosis...

More Information

Publications20

  1. Duran R, Villarino A, Bellinzoni M, Wehenkel A, Fernandez P, Boitel B, et al. Conserved autophosphorylation pattern in activation loops and juxtamembrane regions of Mycobacterium tuberculosis Ser/Thr protein kinases. Biochem Biophys Res Commun. 2005;333:858-67 pubmed
    ..of the entire intracellular regions of four 'receptor-like' protein kinases from Mycobacterium tuberculosis (PknB, PknD, PknE, and PknF), each consisting of an N-terminal kinase domain and a juxtamembrane region of varying length ..
  2. Roumestand C, Leiba J, Galophe N, Margeat E, Padilla A, Bessin Y, et al. Structural insight into the Mycobacterium tuberculosis Rv0020c protein and its interaction with the PknB kinase. Structure. 2011;19:1525-34 pubmed publisher
    ..tuberculosis, also called FhaA, is one of the major substrates of the essential Ser/Thr protein kinase (STPK) PknB. The protein is composed of three domains and is phosphorylated on a unique site in its N terminus...
  3. Anandan T, Han J, Baun H, Nyayapathy S, Brown J, Dial R, et al. Phosphorylation regulates mycobacterial proteasome. J Microbiol. 2014;52:743-54 pubmed publisher
    ..Here, we demonstrate that the α-subunit (PrcA) of the M. tuberculosis proteasome is phosphorylated by the PknB kinase at three threonine residues (T84, T202, and T178) in a sequential manner...
  4. Damle N, Mohanty D. Mechanism of autophosphorylation of mycobacterial PknB explored by molecular dynamics simulations. Biochemistry. 2014;53:4715-26 pubmed publisher
    Mycobacterial Ser/Thr kinase, PknB, is essential for the growth of the pathogen. Unphosphorylated PknB is catalytically inactive, and its activation requires autophosphorylation of Thr residues on the activation loop...
  5. Turapov O, Loraine J, Jenkins C, Barthe P, McFeely D, Forti F, et al. The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth. Open Biol. 2015;5:150025 pubmed publisher
    b>PknB is an essential serine/threonine protein kinase required for mycobacterial cell division and cell-wall biosynthesis...
  6. Kusebauch U, Ortega C, Ollodart A, Rogers R, Sherman D, Moritz R, et al. Mycobacterium tuberculosis supports protein tyrosine phosphorylation. Proc Natl Acad Sci U S A. 2014;111:9265-70 pubmed publisher
    ..Mutation of a phosphotyrosine site of the essential STPK PknB reduces its activity in vitro and in live Mtb, indicating that Tyr phosphorylation has a functional role in ..
  7. Munshi T, Gupta A, Evangelopoulos D, Guzman J, Gibbons S, Keep N, et al. Characterisation of ATP-dependent Mur ligases involved in the biogenesis of cell wall peptidoglycan in Mycobacterium tuberculosis. PLoS ONE. 2013;8:e60143 pubmed publisher
    ..via phosphorylation, we found that all of the Mur synthetases interacted with the Ser/Thr protein kinases, PknA and PknB. In addition, we critically analyzed the interaction network of all of the Mur synthetases with proteins involved ..
  8. Gupta M, Sajid A, Arora G, Tandon V, Singh Y. Forkhead-associated domain-containing protein Rv0019c and polyketide-associated protein PapA5, from substrates of serine/threonine protein kinase PknB to interacting proteins of Mycobacterium tuberculosis. J Biol Chem. 2009;284:34723-34 pubmed publisher
    ..the association of the previously uncharacterized FHA domain-containing protein Rv0019c with cognate STPK PknB. The consequent phosphorylation of Rv0019c is shown to be dependent on the conserved residues in the Rv0019c FHA ..
  9. Gupta A, Pal S, Pandey D, Fakir N, Rathod S, Sinha D, et al. PknB remains an essential and a conserved target for drug development in susceptible and MDR strains of M. Tuberculosis. Ann Clin Microbiol Antimicrob. 2017;16:56 pubmed publisher
    The Mycobacterium tuberculosis (M.tb) protein kinase B (PknB) which is now proved to be essential for the growth and survival of M.tb, is a transmembrane protein with a potential to be a good drug target...
  10. Lombana T, Echols N, Good M, Thomsen N, NG H, Greenstein A, et al. Allosteric activation mechanism of the Mycobacterium tuberculosis receptor Ser/Thr protein kinase, PknB. Structure. 2010;18:1667-77 pubmed publisher
    The essential Mycobacterium tuberculosis Ser/Thr protein kinase (STPK), PknB, plays a key role in regulating growth and division, but the structural basis of activation has not been defined...
  11. Prigozhin D, Papavinasasundaram K, Baer C, Murphy K, Moskaleva A, Chen T, et al. Structural and Genetic Analyses of the Mycobacterium tuberculosis Protein Kinase B Sensor Domain Identify a Potential Ligand-binding Site. J Biol Chem. 2016;291:22961-22969 pubmed
    ..Protein kinase B (PknB) is a transmembrane serine/threonine protein kinase that acts as an essential regulator of mycobacterial growth and ..