Gene Symbol: glbN
Description: hemoglobin GlbN
Species: Mycobacterium tuberculosis H37Rv
Ouellet Y, Daigle R, Lag e P, Dantsker D, Milani M, Bolognesi M, et al
. Ligand binding to truncated hemoglobin N from Mycobacterium tuberculosis is strongly modulated by the interplay between the distal heme pocket residues and internal water. J Biol Chem. 2008;283:27270-8 pubmed publisher
..They also showed that a water molecule is stabilized next to the heme iron through hydrogen-bonding with Tyr(B10) and Gln(E11)...
Choudhary M, Jawaid S, Ahuja M, Shiva N, Gupta P, Bhuyan A, et al
. Open reading frame yjbI of Bacillus subtilis codes for truncated hemoglobin. Protein Expr Purif. 2005;41:363-72 pubmed
..Presumably, only a small portion of the protein is involved in peroxidase activity, which is exposed with increasing concentration of the denaturants. ..
Ouellet Y, Milani M, Couture M, Bolognesi M, Guertin M. Ligand interactions in the distal heme pocket of Mycobacterium tuberculosis truncated hemoglobin N: roles of TyrB10 and GlnE11 residues. Biochemistry. 2006;45:8770-81 pubmed
..These findings suggest that the functional processes of ligand binding and diffusion are controlled in trHbN through the dynamic interaction of residues Y(B10), Q(E11), F(E15), and the heme ligand...
Bidon Chanal A, Marti M, Estrin D, Luque F. Dynamical regulation of ligand migration by a gate-opening molecular switch in truncated hemoglobin-N from Mycobacterium tuberculosis. J Am Chem Soc. 2007;129:6782-8 pubmed
..The results support a molecular mechanism evolved to ensure access of NO to the heme cavity in the oxygenated form of the protein, which should warrant survival of the microorganism under stress conditions. ..
Koebke K, Waletzko M, Pacheco A. Direct Monitoring of the Reaction between Photochemically Generated Nitric Oxide and Mycobacterium tuberculosis Truncated Hemoglobin N Wild Type and Variant Forms: An Assessment of Computational Mechanistic Predictions. Biochemistry. 2016;55:686-96 pubmed publisher
Daigle R, Guertin M, Lag e P. Structural characterization of the tunnels of Mycobacterium tuberculosis truncated hemoglobin N from molecular dynamics simulations. Proteins. 2009;75:735-47 pubmed publisher
..We propose that the EH and the long tunnels are used for apolar ligands storage. The trajectories bring important new structural insights related to trHbN function and to ligand diffusion in proteins...
Savard P, Daigle R, Morin S, Sebilo A, Meindre F, Lag e P, et al
. Structure and dynamics of Mycobacterium tuberculosis truncated hemoglobin N: insights from NMR spectroscopy and molecular dynamics simulations. Biochemistry. 2011;50:11121-30 pubmed publisher
..This work constitutes the first study of a truncated hemoglobin dynamics performed by solution heteronuclear relaxation NMR spectroscopy...
Milani M, Pesce A, Ouellet Y, Ascenzi P, Guertin M, Bolognesi M. Mycobacterium tuberculosis hemoglobin N displays a protein tunnel suited for O2 diffusion to the heme. EMBO J. 2001;20:3902-9 pubmed
..It is proposed that in trHbN, heme Fe/O2 stereochemistry and the protein matrix tunnel may promote O2/NO chemistry in vivo, as a M.tuberculosis defense mechanism against macrophage nitrosative stress. ..
Milani M, Pesce A, Ouellet Y, Dewilde S, Friedman J, Ascenzi P, et al
. Heme-ligand tunneling in group I truncated hemoglobins. J Biol Chem. 2004;279:21520-5 pubmed