esxB

Summary

Gene Symbol: esxB
Description: ESAT-6-like protein EsxB
Alias: cfp10, lhp
Species: Mycobacterium tuberculosis H37Rv
Products:     esxB

Top Publications

  1. Renshaw P, Lightbody K, Veverka V, Muskett F, Kelly G, Frenkiel T, et al. Structure and function of the complex formed by the tuberculosis virulence factors CFP-10 and ESAT-6. EMBO J. 2005;24:2491-8 pubmed
  2. Okkels L, Müller E, Schmid M, Rosenkrands I, Kaufmann S, Andersen P, et al. CFP10 discriminates between nonacetylated and acetylated ESAT-6 of Mycobacterium tuberculosis by differential interaction. Proteomics. 2004;4:2954-60 pubmed
    ..In 2-DE blot overlay assays, the recombinant 10 kDa culture filtrate protein (CFP10) discriminated N-terminal acetylated and nonacetylated ESAT-6 by differential interaction, whereas removal of the ..
  3. Champion P, Stanley S, Champion M, Brown E, Cox J. C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. Science. 2006;313:1632-6 pubmed
    ..Attachment of the signal to yeast ubiquitin was sufficient for secretion from M. tuberculosis cells, demonstrating that this ESX-1 signal is portable. ..
  4. Berthet F, Rasmussen P, Rosenkrands I, Andersen P, Gicquel B. A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10). Microbiology. 1998;144 ( Pt 11):3195-203 pubmed
    ..The promoter region of M. tuberculosis esat-6 was cloned and characterized. A new gene, designated lhp and cotranscribed with esat-6, was identified. Moreover, computer searches in the M...
  5. Lu J, Zou L, Liu B, Li X, Tan J, Zhao A, et al. Development of a time-resolved fluoroimmunoassay of CFP-10 for rapid diagnosis of tuberculous pleural effusion. Tuberculosis (Edinb). 2015;95:426-31 pubmed publisher
    ..Therefore, the TRF of CFP-10 may be used for the rapid diagnosis of tuberculous pleural effusion and further monitoring the clinical therapeutic efficacy of tuberculosis. ..
  6. Refaya A, Sivakumar S, Sundararaman B, Narayanan S. Polymorphism in the RD1 locus and its effect on downstream genes among South Indian clinical isolates of Mycobacterium tuberculosis. J Med Microbiol. 2012;61:1352-9 pubmed publisher
    ..These data suggest that changes in transcription levels resulting from insertion of different copy numbers of the repeat element may affect regulation of gene expression in M. tuberculosis. ..
  7. Sinha A, Singh A, Satchidanandam V, Natarajan K. Impaired generation of reactive oxygen species during differentiation of dendritic cells (DCs) by Mycobacterium tuberculosis secretory antigen (MTSA) and subsequent activation of MTSA-DCs by mycobacteria results in increased intracellular survival. J Immunol. 2006;177:468-78 pubmed
    ..bovis BCG. Overall our results suggest that DCs differentiated with Ags such as MTSA may provide a niche for survival and/or growth of mycobacteria following sequestration of ROS. ..
  8. Das K, Thomas T, Garnica O, Dhandayuthapani S. Recombinant Bacillus subtilis spores for the delivery of Mycobacterium tuberculosis Ag85B-CFP10 secretory antigens. Tuberculosis (Edinb). 2016;101S:S18-S27 pubmed publisher
    ..subtilis spores for the expression of two major immunodominant antigens of Mycobacterium tuberculosis, Ag85B and CFP10. We created three recombinant B...
  9. Meher A, Bal N, Chary K, Arora A. Mycobacterium tuberculosis H37Rv ESAT-6-CFP-10 complex formation confers thermodynamic and biochemical stability. FEBS J. 2006;273:1445-62 pubmed
    ..The ITC and thermal unfolding approach described in this study can readily be applied to characterization of the 11 other pairs of ESAT-6 family proteins and for screening ESAT-6 and CFP-10 mutants. ..

More Information

Publications17

  1. Callahan B, Nguyen K, Collins A, Valdes K, Caplow M, Crossman D, et al. Conservation of structure and protein-protein interactions mediated by the secreted mycobacterial proteins EsxA, EsxB, and EspA. J Bacteriol. 2010;192:326-35 pubmed publisher
    Mycobacterium tuberculosis EsxA and EsxB proteins are founding members of the WXG100 (WXG) protein family, characterized by their small size (approximately 100 amino acids) and conserved WXG amino acid motif. M...
  2. Champion P, Champion M, Manzanillo P, Cox J. ESX-1 secreted virulence factors are recognized by multiple cytosolic AAA ATPases in pathogenic mycobacteria. Mol Microbiol. 2009;73:950-62 pubmed publisher
    ..Ultimately, understanding the details of ESX-1 targeting may allow for engineering of better vaccines to prevent tuberculosis. ..
  3. Murthy M, Kaliappan T, Raja A. Cytokine and chemokine responses to selected early secreted antigenic target-6 and culture filtrate protein-10 peptides in tuberculosis. J Interferon Cytokine Res. 2011;31:299-307 pubmed publisher
    ..Monocyte chemoattractant protein-1 positive monocytes increased in response to the peptides Esp1, Esp6, Cfp8, and Cfp9 in PTB. These peptides deserve attention for further immune studies. ..
  4. Soo P, Horng Y, Chen A, Yang S, Chang K, Lee J, et al. Validation of nanodiamond-extracted CFP-10 antigen as a biomarker in clinical isolates of Mycobacterium tuberculosis complex in broth culture media. Tuberculosis (Edinb). 2015;95:620-4 pubmed publisher
    ..6 and the CFP-10 antigen could maintain good signal intensity for a week. Our results suggest that, with the DND MALDI-TOF mass spectrometry approach, CFP-10 antigen can be used as an early diagnosis biomarker in clinical practice. ..
  5. Yang H, Chen H, Liu Z, Ma H, Qin L, Jin R, et al. A novel B-cell epitope identified within Mycobacterium tuberculosis CFP10/ESAT-6 protein. PLoS ONE. 2013;8:e52848 pubmed publisher
    The 10-kDa culture filtrate protein (CFP10) and 6-kDa early-secreted target antigen (ESAT-6) play important roles in mycobacterial virulence and pathogenesis through a 1:1 complex formation (CFP10/ESAT-6 protein, CE protein), which have ..
  6. McLaughlin B, Chon J, MacGurn J, Carlsson F, Cheng T, Cox J, et al. A mycobacterium ESX-1-secreted virulence factor with unique requirements for export. PLoS Pathog. 2007;3:e105 pubmed
    ..The results establish EspB as a substrate of ESX-1 that is required for virulence and growth in macrophages and suggests that the contribution of ESX-1 to virulence may arise from the secretion of multiple independent substrates. ..
  7. Kimuda S, Andia Biraro I, Egesa M, Bagaya B, Raynes J, Levin J, et al. Use of QuantiFERON®-TB Gold in-tube culture supernatants for measurement of antibody responses. PLoS ONE. 2017;12:e0188396 pubmed publisher
    ..tb specific cytokines. Further antibody and antibody/cytokine combinations need to be explored to achieve better diagnostic accuracy. ..
  8. Chen J, Zhang M, Rybniker J, Basterra L, Dhar N, Tischler A, et al. Phenotypic profiling of Mycobacterium tuberculosis EspA point mutants reveals that blockage of ESAT-6 and CFP-10 secretion in vitro does not always correlate with attenuation of virulence. J Bacteriol. 2013;195:5421-30 pubmed publisher
    ..However, our data also indicate for the first time that blockage of M. tuberculosis ESAT-6 and CFP-10 secretion in vitro and attenuation are mutually exclusive...