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| MRC1SummaryGene Symbol: MRC1 Description: Mrc1p Alias: YCL060C Species: Top Publications
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Publications
Mrc1, Tof1 and Csm3 inhibit CAG.CTG repeat instability by at least two mechanismsDavid F Razidlo
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Box 986805, Omaha, NE 68198 6805, USA
DNA Repair (Amst) 7:633-40. 2008..CTG repeat contraction rates were sought using a disruption library. This screen identified Mrc1, the homolog of human Claspin, which mediates the replication and DNA damage checkpoints, and also couples the ..- Mrc1 and DNA polymerase epsilon function together in linking DNA replication and the S phase checkpointHuiqiang Lou
Braun Laboratories, California Institute of Technology, Pasadena, CA 91125, USA
Mol Cell 32:106-17. 2008Yeast Mrc1, ortholog of metazoan Claspin, is both a central component of normal DNA replication forks and a mediator of the S phase checkpoint... - Mrc1 is a replication fork component whose phosphorylation in response to DNA replication stress activates Rad53Alexander J Osborn
Verna and Marrs MacLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
Genes Dev 17:1755-67. 2003..In budding yeast, this pathway includes the kinases Mec1 and Rad53. Here we report that the Mediator protein Mrc1, which is required for normal DNA replication and for activation of Rad53, is present at replication forks... - Genetic dissection of parallel sister-chromatid cohesion pathwaysHong Xu
Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5S 1A8, Canada
Genetics 176:1417-29. 2007..These data defined two cohesion pathways, one containing CSM3, TOF1, CTF4, and CHL1, and the second containing MRC1, CTF18, CTF8, and DCC1... - Mrc1 is required for normal progression of replication forks throughout chromatin in S. cerevisiaeShawn J Szyjka
Molecular and Computational Biology Program, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089, USA
Mol Cell 19:691-7. 2005b>Mrc1 associates with replication forks, where it transmits replication stress signals and is required for normal replisome pausing in response to nucleotide depletion... - Mrc1, a non-essential DNA replication protein, is required for telomere end protection following loss of capping by Cdc13, Yku or telomeraseNathalie Grandin
UMR CNRS no 5161, Ecole Normale Superieure de Lyon, IFR128 BioSciences Gerland, 46, Allee d Italie, 69364 Lyon, France
Mol Genet Genomics 277:685-99. 2007..Here, we report that in the absence of Mrc1, a component of the replication forks, telomeres of cdc13 or yku70 mutants exhibited increased degradation, while .. - The Tof1p-Csm3p protein complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiaeBidyut K Mohanty
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
Proc Natl Acad Sci U S A 103:897-902. 2006..Other genes such as MRC1, WSS1, and PSY2 that are also involved in the MRC1 checkpoint pathway were not involved in this global control... - Mrc1 and Tof1 regulate DNA replication forks in different ways during normal S phaseBen Hodgson
Cancer Research U K, Paterson Institute for Cancer Research, University of Manchester, Manchester M20 4BX, United Kingdom
Mol Biol Cell 18:3894-902. 2007The Mrc1 and Tof1 proteins are conserved throughout evolution, and in budding yeast they are known to associate with the MCM helicase and regulate the progression of DNA replication forks... - Mrc1 transduces signals of DNA replication stress to activate Rad53A A Alcasabas
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
Nat Cell Biol 3:958-65. 2001..We describe a conserved protein Mrc1, mediator of the replication checkpoint, required for activation of ScRad53 and SpCds1 during replication stress... - Mrc1 and Tof1 promote replication fork progression and recovery independently of Rad53Hélène Tourrière
Institute of Human Genetics, Centre National de la Recherche Scientifique, UPR 1142, 34396 Montpellier Cedex 5, France
Mol Cell 19:699-706. 2005..The critical role of Mrc1p in HU is therefore to promote fork recovery in a Rad53p-independent manner, presumably through the formation of a stable fork-pausing complex... - S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complexYuki Katou
Genome Structure and Function Team, Human Genome Research Group, RIKEN Genomic Science Center, 1 7 22 Suehiro cho, Japan
Nature 424:1078-83. 2003..Here we demonstrate that the checkpoint regulatory proteins Tof1 and Mrc1 interact directly with the DNA replication machinery in Saccharomyces cerevisiae... - Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication forkArturo Calzada
Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK
Genes Dev 19:1905-19. 2005..The budding yeast proteins Mrc1 and Tof1 associate with the putative MCM-Cdc45 helicase and limit progression of the replisome when nucleotides are .. - Reconstitution of Rad53 activation by Mec1 through adaptor protein Mrc1Sheng Hong Chen
Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093 0653, USA
J Biol Chem 284:18593-604. 2009..Using an activity-based assay for Rad53, we found that Mrc1, a replication fork-associated protein, cooperates with Mec1 to activate Rad53 directly... - Differential regulation of homologous recombination at DNA breaks and replication forks by the Mrc1 branch of the S-phase checkpointConstance Alabert
Department of Genome Dynamics, Institute of Human Genetics, CNRS UPR 1142, Montpellier, France
EMBO J 28:1131-41. 2009..to replication stress also depends on the Mec1 kinase, which activates the DNA replication checkpoint in an Mrc1-dependent manner in response to fork arrest... - Contrasting roles of checkpoint proteins as recombination modulators at Fob1-Ter complexes with or without fork arrestBidyut K Mohanty
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
Eukaryot Cell 8:487-95. 2009..Other checkpoint proteins of the checkpoint adapter/mediator class such as Mrc1 and Rad9, which channel signals from the sensor to the effector kinase, tended to suppress recombination at Fob1-.. - A SUMO-like domain protein, Esc2, is required for genome integrity and sister chromatid cohesion in Saccharomyces cerevisiaeTomoko Ohya
Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
Genetics 180:41-50. 2008.... - Replication stalling at unstable inverted repeats: interplay between DNA hairpins and fork stabilizing proteinsIrina Voineagu
Department of Biology, Tufts University, Medford, MA 02155, USA
Proc Natl Acad Sci U S A 105:9936-41. 2008..Finally, we report that yeast fork-stabilizing proteins, Tof1 and Mrc1, are required to counteract repeat-mediated replication stalling... - Orchestration of the S-phase and DNA damage checkpoint pathways by replication forks from early originsJulie M Caldwell
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
J Cell Biol 180:1073-86. 2008..Thus, oncogene-mediated deregulation of cyclins in the early stages of cancer development could contribute to genomic instability through a deficiency in the forks required to establish the S-phase checkpoint... - Mrc1 phosphorylation in response to DNA replication stress is required for Mec1 accumulation at the stalled forkMaria L Naylor
Department of Genetics, Harvard Medical School, Division of Genetics, Brigham and Women s Hospital, Howard Hughes Medical Institute, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 106:12765-70. 2009..The budding yeast Mec1 sensor kinase, Mrc1 mediator, and Rad53 effector kinase are central to this signal transduction cascade in S phase... - The direct binding of Mrc1, a checkpoint mediator, to Mcm6, a replication helicase, is essential for the replication checkpoint against methyl methanesulfonate-induced stressMakiko Komata
Laboratory of Chromosome Structure and Function, Department of Biological Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama City, Kanagawa, Japan
Mol Cell Biol 29:5008-19. 2009b>Mrc1 plays a role in mediating the DNA replication checkpoint. We surveyed replication elongation proteins that interact directly with Mrc1 and identified a replicative helicase, Mcm6, as a specific Mrc1-binding protein... - Inviability of a DNA2 deletion mutant is due to the DNA damage checkpointMartin E Budd
California Institute of Technology, Pasadena, CA USA
Cell Cycle 10:1690-8. 2011..of a novel, spontaneously arising suppressor of dna2? now reveals that mutation of rad9 and double mutation of rad9 mrc1 can also suppress the lethality of dna2? mutants... - New functions of Ctf18-RFC in preserving genome stability outside its role in sister chromatid cohesionLionel Gellon
Department of Biology, Tufts University, Medford, Massachusetts, United States of America
PLoS Genet 7:e1001298. 2011..First, genetic instability in mutants of Ctf18-RFC was exacerbated by simultaneous deletion of the fork stabilizer Mrc1, but suppressed by deletion of the repair protein Rad52... - Ploidy dictates repair pathway choice under DNA replication stressXin Chenglin Li
Department of Molecular Biology and Genetics, College of Agriculture and Life Sciences, Cornell University, Ithaca, New York 14853, USA
Genetics 187:1031-40. 2011..In response to replication stress, early events associated with ploidy dictate the repair pathway choice. This study uncovers a fundamental difference between haplophase and diplophase in the maintenance of genome integrity... - Post-replication repair suppresses duplication-mediated genome instabilityChristopher D Putnam
Ludwig Institute for Cancer Research, University of California San Diego School of Medicine, La Jolla, California, United States of America
PLoS Genet 6:e1000933. 2010..Our analysis is consistent with models in which PRR prevents replication damage from becoming double strand breaks (DSBs) and/or regulates the activity of HR on DSBs... - MRC1-dependent scaling of the budding yeast DNA replication timing programAmnon Koren
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Genome Res 20:781-90. 2010..In sharp contrast, cells deleted of MRC1, a gene implicated in replication fork stabilization and in the replication checkpoint pathway, maintained wild-.. - The amino-terminal TPR domain of Dia2 tethers SCF(Dia2) to the replisome progression complexHiroko Morohashi
Cancer Research UK Paterson Institute for Cancer Research, University of Manchester, UK
Curr Biol 19:1943-9. 2009..This interaction requires the RPC components Mrc1 and Ctf4, both of which associate with a tetratricopeptide repeat (TPR) domain located at the amino terminus of .. - SCF(Dia2) regulates DNA replication forks during S-phase in budding yeastSatoru Mimura
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya, Japan
EMBO J 28:3693-705. 2009..Using modified yeast two-hybrid screening, we have identified components of the replisome (Mrc1, Ctf4 and Mcm2), as Dia2-binding proteins. Mrc1 and Ctf4 were ubiquitinated by SCF(Dia2) both in vivo and in vitro... - Csm3, Tof1, and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forksMasashige Bando
Department of Biological Science, Laboratory of Chromosome Structure and Function, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, B 20, 4259, Nagatsuta, Midori ku, Yokohama City, Kanagawa, 226 8501, Japan
J Biol Chem 284:34355-65. 2009b>Mrc1 (mediator of replication checkpoint), Tof1 (topoisomerase I interacting factor), and Csm3 (chromosome segregation in meiosis) are checkpoint-mediator proteins that function during DNA replication and activate the effector kinase .. - The S-phase checkpoint is required to respond to R-loops accumulated in THO mutantsBelén Gómez-González
Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Seville, Spain
Mol Cell Biol 29:5203-13. 2009..In light of these results, we propose a model in which R-loop-mediated recombination is explained by template switching... - Cellular morphogenesis under stress is influenced by the sphingolipid pathway gene ISC1 and DNA integrity checkpoint genes in Saccharomyces cerevisiaeKaushlendra Tripathi
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
Genetics 189:533-47. 2011..That is, yeast carrying deletions of both ISC1 and a replication checkpoint mediator gene including MRC1, TOF1, or CSM3 display basal morphological defects, which increase following HU treatment... - Ccr4 contributes to tolerance of replication stress through control of CRT1 mRNA poly(A) tail lengthRobert N Woolstencroft
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, Canada
J Cell Sci 119:5178-92. 2006..These results implicate the coordinated regulation of Crt1 via Ccr4 and Dun1 as a crucial nodal point in the response to DNA replication stress... - Dominant TEL1-hy mutations compensate for Mec1 lack of functions in the DNA damage responseVeronica Baldo
Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, P zza della Scienza 2, 20126 Milan, Italy
Mol Cell Biol 28:358-75. 2008..Finally, Tel1-hy544 can activate the checkpoint more efficiently than wild-type Tel1, while it causes telomere shortening, indicating that the checkpoint and telomeric functions of Tel1 can be separable... - Abrogation of the Chk1-Pds1 checkpoint leads to tolerance of persistent single-strand breaks in Saccharomyces cerevisiaeAnandi S Karumbati
Department of Pathology, University of Michigan Medical School, Ann Arbor, 48109 0602, USA
Genetics 169:1833-44. 2005..We propose a model in which recombinational repair during S phase coupled with failure of the metaphase-anaphase checkpoint allows for tolerance of persistent single-strand breaks at the expense of genome stability... - Mechanistically distinct roles for Sgs1p in checkpoint activation and replication fork maintenanceLotte Bjergbaek
Department of Molecular Biology and NCCR, Frontiers in Genetics, University of Geneva, Quai Ernest Ansermet 30, Geneva, Switzerland
EMBO J 24:405-17. 2005..function, the Sgs1p/Top3p complex acts in parallel to the Claspin-related adaptor, Mrc1p, although the sgs1 and mrc1 mutations are epistatic for Rad53p activation... - Loss of rereplication control in Saccharomyces cerevisiae results in extensive DNA damageBrian M Green
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143 2200, USA
Mol Biol Cell 16:421-32. 2005..In contrast, Mrc1, a checkpoint protein required for recognition of replication stress, does not play a role in the response to .. - Ccr4-not complex mRNA deadenylase activity contributes to DNA damage responses in Saccharomyces cerevisiaeAna Traven
St Vincent s Institute of Medical Research, Fitzroy, Victoria 3065, Australia
Genetics 169:65-75. 2005..the major cytoplasmic mRNA deadenylase complex, have complex genetic interactions with the checkpoint genes DUN1, MRC1, RAD9, and RAD17 in response to DNA-damaging agents hydroxyurea (HU) and methylmethane sulfonate (MMS)... - Rad53 kinase activation-independent replication checkpoint function of the N-terminal forkhead-associated (FHA1) domainBrietta L Pike
St Vincent s Institute of Medical Research, Department of Medicine, The University of Melbourne, Fitzroy, Victoria, Australia
J Biol Chem 279:39636-44. 2004..an important mechanistic difference to the homologous Schizosaccharomyces pombe FHA domain that is required for Mrc1-dependent activation of the corresponding Cds1 kinase... - Saccharomyces cerevisiae Rrm3p DNA helicase promotes genome integrity by preventing replication fork stalling: viability of rrm3 cells requires the intra-S-phase checkpoint and fork restart activitiesJorge Z Torres
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
Mol Cell Biol 24:3198-212. 2004..The rrm3 system provides a unique opportunity to learn the fate of forks whose progress is impaired by natural impediments rather than by exogenous DNA damage... - The role of Cdh1p in maintaining genomic stability in budding yeastKaren E Ross
The Laboratory of Molecular and Cellular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Genetics 165:489-503. 2003..We propose that the failure to degrade cyclins at the end of mitosis leaves cdh1delta mutant strains with abnormal Cdc28p/Clb activity that interferes with proper chromosome segregation... - DNA helicase gene interaction network defined using synthetic lethality analyzed by microarraySiew Loon Ooi
Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, 617 Hunterian Building, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
Nat Genet 35:277-86. 2003..SGS1 and SRS2 have synthetic defects with MRC1 but not RAD9, suggesting that SGS1 and SRS2 function in a parallel pathway with MRC1 to transduce the DNA .. - Uncoupling of unwinding from DNA synthesis implies regulation of MCM helicase by Tof1/Mrc1/Csm3 checkpoint complexMarina N Nedelcheva
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria
J Mol Biol 347:509-21. 2005..In concordance with this suggestion, we found that the Tof1/Csm3/Mrc1 checkpoint complex interacts directly with the MCM helicase during both replication fork progression and when the .. - RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complexMichael Chang
Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada
EMBO J 24:2024-33. 2005..In addition, rmi1Delta strains fail to fully activate Rad53 upon exposure to DNA-damaging agents, suggesting that Rmi1 is also an important part of the Rad53-dependent DNA damage response... - Genetic analysis of Saccharomyces cerevisiae H2A serine 129 mutant suggests a functional relationship between H2A and the sister-chromatid cohesion partners Csm3-Tof1 for the repair of topoisomerase I-induced DNA damageChristophe Redon
NIH, NCI, DBS, Laboratory of Molecular Pharmacology, Bethesda, Maryland 20892, USA
Genetics 172:67-76. 2006..We now report that efficient repair also requires proteins involved in chromatid cohesion: Csm3; Tof1; Mrc1, and Dcc1. Epistasis analysis defined several pathways involving these proteins... - Mrc1 protects uncapped budding yeast telomeres from exonuclease EXO1Avgi Tsolou
Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Henry Wellcome Laboratory for Biogerontology Research, Newcastle University, Newcastle upon Tyne NE4 6BE, UK
DNA Repair (Amst) 6:1607-17. 2007b>Mrc1 (Mediator of Replication Checkpoint 1) is a component of the DNA replication fork machinery and is necessary for checkpoint activation after replication stress. In this study, we addressed the role of Mrc1 at uncapped telomeres... - Local chromatin structure at the ribosomal DNA causes replication fork pausing and genome instability in the absence of the S. cerevisiae DNA helicase Rrm3pJorge Z Torres
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
Genes Dev 18:498-503. 2004.... - Suppression of spontaneous genome rearrangements in yeast DNA helicase mutantsKristina H Schmidt
Ludwig Institute for Cancer Research, University of California at San Diego, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 103:18196-201. 2006..DNA damage sensors and results from activation of the Mec1/Rad9-dependent DNA damage response rather than the Mrc1-dependent replication stress response... - Mgs1 and Rad18/Rad5/Mms2 are required for survival of Saccharomyces cerevisiae mutants with novel temperature/cold sensitive alleles of the DNA polymerase delta subunit, Pol31Niloofar Davoodi Vijeh Motlagh
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Sendai, Miyagi 980 8578, Japan
DNA Repair (Amst) 5:1459-74. 2006..Deletion of SGS1, RAD52, SRS2, MRC1 or RAD24 had a deleterious effect only in combination with those pol31 alleles that had a phenotype as single .. - Regulation of DNA replication machinery by Mrc1 in fission yeastNaoki Nitani
Department of Biological Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
Genetics 174:155-65. 2006..b>Mrc1 is required to activate Cds1 and prevent the replication machinery from uncoupling from DNA synthesis... - Mrc1 and Srs2 are major actors in the regulation of spontaneous crossoverThomas Robert
Commissariat a l Energie Atomique, UMR217 CNRS CEA DSV DRR SRMC LERA, Fontenay aux Roses, France
EMBO J 25:2837-46. 2006..Our results support the view that Mrc1 plays a specific role in DNA replication, promoting the Srs2 recruitment to PCNA independently of checkpoint .. - A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recoveryMichael Christopher Keogh
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 439:497-501. 2006..The dephosphorylation of gammaH2AX by the HTP-C is necessary for efficient recovery from the DNA damage checkpoint... - Asf1 links Rad53 to control of chromatin assemblyF Hu
Verna and Mars McLean Department of Biochemistry and Molecular Biology, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
Genes Dev 15:1061-6. 2001..Thus, checkpoint pathways directly regulate chromatin assembly to promote survival in response to DNA damage and replication blocks... - GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forksAgnieszka Gambus
Cancer Research UK, Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, M20 4BX, UK
Nat Cell Biol 8:358-66. 2006..RPC components include the essential initiation and elongation factor, Cdc45, the checkpoint mediator Mrc1, the Tof1-Csm3 complex that allows replication forks to pause at protein-DNA barriers, the histone chaperone FACT (..
Research Grants
- Suppression of translocations by RecQ-like DNA helicasesKRISTINA HILDEGARD SCHMIDT; Fiscal Year: 2010....
- Suppression of translocations by RecQ-like DNA helicasesKRISTINA HILDEGARD SCHMIDT; Fiscal Year: 2010....