Gene Symbol: Ybx2
Description: Y box protein 2
Alias: Msy2, Y-box-binding protein 2, Y-box protein MSY2, germ cell-specific Y-box-binding protein
Species: mouse
Products:     Ybx2

Top Publications

  1. Giorgini F, Davies H, Braun R. MSY2 and MSY4 bind a conserved sequence in the 3' untranslated region of protamine 1 mRNA in vitro and in vivo. Mol Cell Biol. 2001;21:7010-9 pubmed
    ..shown that a sequence-specific RNA binding activity present in spermatogenic cells contains the two Y-box proteins MSY2 and MSY4...
  2. Gu W, Tekur S, Reinbold R, Eppig J, Choi Y, Zheng J, et al. Mammalian male and female germ cells express a germ cell-specific Y-Box protein, MSY2. Biol Reprod. 1998;59:1266-74 pubmed
    ..homologue of the Xenopus germ cell-specific nucleic acid-binding protein FRGY2 (mRNP3+4), hereafter designated MSY2. MSY2 is a member of the Y box multigene family of proteins; it contains the cold shock domain that is highly ..
  3. Lu Z, Books J, Ley T. Cold shock domain family members YB-1 and MSY4 share essential functions during murine embryogenesis. Mol Cell Biol. 2006;26:8410-7 pubmed
    Three cold shock domain (CSD) family members (YB-1, MSY2, and MSY4) exist in vertebrate species ranging from frogs to humans...
  4. Yang J, Medvedev S, Yu J, Tang L, Agno J, Matzuk M, et al. Absence of the DNA-/RNA-binding protein MSY2 results in male and female infertility. Proc Natl Acad Sci U S A. 2005;102:5755-60 pubmed
    b>MSY2, a germ-cell-specific member of the Y-box family of DNA-/RNA-binding proteins, is proposed to function as a coactivator of transcription in the nucleus and to stabilize and store maternal and paternal mRNAs in the cytoplasm...
  5. Yu J, Hecht N, Schultz R. RNA-binding properties and translation repression in vitro by germ cell-specific MSY2 protein. Biol Reprod. 2002;67:1093-8 pubmed
    The large amount of MSY2 protein, a mouse germ cell-specific Y-box protein, in oocytes and its degradation by the late two-cell stage suggest that MSY2 may stabilize and/or regulate the translation of maternal mRNAs...
  6. Yang J, Medvedev S, Reddi P, Schultz R, Hecht N. The DNA/RNA-binding protein MSY2 marks specific transcripts for cytoplasmic storage in mouse male germ cells. Proc Natl Acad Sci U S A. 2005;102:1513-8 pubmed
    ..The germ cell Y-box protein, MSY2, constituting approximately 0...
  7. Davies H, Giorgini F, Fajardo M, Braun R. A sequence-specific RNA binding complex expressed in murine germ cells contains MSY2 and MSY4. Dev Biol. 2000;221:87-100 pubmed
    ..by electrophoretic mobility shift assays and immunoprecipitation indicates the activity is composed of MSY4 and MSY2, another mouse Y box protein...
  8. Cullinane D, Chowdhury T, Kleene K. Mechanisms of translational repression of the Smcp mRNA in round spermatids. Reproduction. 2015;149:43-54 pubmed publisher
    ..RNA affinity chromatography and mass spectrometry sequencing identified Y-box protein 2 (YBX2/MSY2) as the major protein that interacts with the 3' terminus of the Smcp 3'-UTR and a Y-box recognition sequence, ..
  9. Yang J, Morales C, Medvedev S, Schultz R, Hecht N. In the absence of the mouse DNA/RNA-binding protein MSY2, messenger RNA instability leads to spermatogenic arrest. Biol Reprod. 2007;76:48-54 pubmed
    b>MSY2 is a member of the Y-box family of proteins solely expressed in male and female germ cells...

More Information


  1. Snyder E, Soundararajan R, Sharma M, Dearth A, Smith B, Braun R. Compound Heterozygosity for Y Box Proteins Causes Sterility Due to Loss of Translational Repression. PLoS Genet. 2015;11:e1005690 pubmed publisher
    The Y-box proteins YBX2 and YBX3 bind RNA and DNA and are required for metazoan development and fertility...
  2. Kleene K. Position-dependent interactions of Y-box protein 2 (YBX2) with mRNA enable mRNA storage in round spermatids by repressing mRNA translation and blocking translation-dependent mRNA decay. Mol Reprod Dev. 2016;83:190-207 pubmed publisher
    ..Two lines of evidence implicate the highly abundant and well-known translational repressor, Y-box protein 2 (YBX2), as a critical factor: First, protamine 1 (Prm1) and sperm-mitochondria cysteine-rich protein (Smcp) mRNAs are ..
  3. Krentz A, Murphy M, Sarver A, Griswold M, Bardwell V, Zarkower D. DMRT1 promotes oogenesis by transcriptional activation of Stra8 in the mammalian fetal ovary. Dev Biol. 2011;356:63-70 pubmed publisher
    ..Thus DMRT1 controls Stra8 sex-specifically, activating it in the fetal ovary and repressing it in the adult testis. ..
  4. Yu J, Hecht N, Schultz R. Expression of MSY2 in mouse oocytes and preimplantation embryos. Biol Reprod. 2001;65:1260-70 pubmed
    ..b>MSY2, a member of a multifunctional Y-box protein family, is implicated in repressing the translation of paternal mRNAs...
  5. Medvedev S, Yang J, Hecht N, Schultz R. CDC2A (CDK1)-mediated phosphorylation of MSY2 triggers maternal mRNA degradation during mouse oocyte maturation. Dev Biol. 2008;321:205-15 pubmed publisher
    ..Messenger RNA is extremely stable during oocyte growth in mouse and MSY2, an abundant germ cell-specific RNA-binding protein, likely serves as a mediator of global mRNA stability...
  6. Xu M, Medvedev S, Yang J, Hecht N. MIWI-independent small RNAs (MSY-RNAs) bind to the RNA-binding protein, MSY2, in male germ cells. Proc Natl Acad Sci U S A. 2009;106:12371-6 pubmed publisher
    The germ cell-specific DNA/RNA-binding protein MSY2 binds small RNAs (MSY-RNAs) that are approximately 25-31 nt in length, often initiate with a 5' adenine, and are expressed in both germ cells and somatic cells...
  7. Bailey A, Batista P, Gold R, Chen Y, de Rooij D, Chang H, et al. The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline. elife. 2017;6: pubmed publisher
  8. Flemr M, Ma J, Schultz R, Svoboda P. P-body loss is concomitant with formation of a messenger RNA storage domain in mouse oocytes. Biol Reprod. 2010;82:1008-17 pubmed publisher
    ..by loss of P-bodies and a subcortical accumulation of several RNA-binding proteins, including DDX6, CPEB, YBX2 (MSY2), and the exon junction complex...
  9. Xu D, Xu S, Kyaw A, Lim Y, Chia S, Chee Siang D, et al. RNA Binding Protein Ybx2 Regulates RNA Stability During Cold-Induced Brown Fat Activation. Diabetes. 2017;66:2987-3000 pubmed publisher
    ..role of RBPs in BAT, we profiled >400 RBPs in different adipose depots and identified Y-box binding protein 2 (Ybx2) as a novel regulator in BAT activation...
  10. Ottolenghi C, Omari S, Garcia Ortiz J, Uda M, Crisponi L, Forabosco A, et al. Foxl2 is required for commitment to ovary differentiation. Hum Mol Genet. 2005;14:2053-62 pubmed
    ..This suggests the possible continued involvement of sex-determining genes in maintaining ovarian function throughout female reproductive life. ..
  11. Yu J, Deng M, Medvedev S, Yang J, Hecht N, Schultz R. Transgenic RNAi-mediated reduction of MSY2 in mouse oocytes results in reduced fertility. Dev Biol. 2004;268:195-206 pubmed
    b>MSY2 is implicated in regulating the stability and translation of maternal mRNAs during mouse oogenesis...
  12. Pangas S, Choi Y, Ballow D, Zhao Y, Westphal H, Matzuk M, et al. Oogenesis requires germ cell-specific transcriptional regulators Sohlh1 and Lhx8. Proc Natl Acad Sci U S A. 2006;103:8090-5 pubmed
    ..In addition, we show that Lhx8 is downstream of Sohlh1 and critical in fertility. Thus, Sohlh1 and Lhx8 are two germ cell-specific, critical regulators of oogenesis. ..
  13. Yu J, Hecht N, Schultz R. Requirement for RNA-binding activity of MSY2 for cytoplasmic localization and retention in mouse oocytes. Dev Biol. 2003;255:249-62 pubmed
    b>MSY2, a mouse germ cell-specific Y-box protein, is implicated in the global regulation of the stability and/or translation of maternal mRNAs in the mouse oocyte...
  14. Liu X, Morency E, Li T, Qin H, Zhang X, Zhang X, et al. Role for PADI6 in securing the mRNA-MSY2 complex to the oocyte cytoplasmic lattices. Cell Cycle. 2017;16:360-366 pubmed publisher
    ..Consistent with this observation, the amount of the major RNA binding protein, MSY2, that is associated with the insoluble fraction of the oocytes after Triton X-100 extraction is also markedly ..
  15. Soyal S, Amleh A, Dean J. FIGalpha, a germ cell-specific transcription factor required for ovarian follicle formation. Development. 2000;127:4645-54 pubmed
    ..The persistence of FIGalpha in adult females suggests that it may regulate additional pathways that are essential for normal ovarian development. ..
  16. Herbert T, Hecht N. The mouse Y-box protein, MSY2, is associated with a kinase on non-polysomal mouse testicular mRNAs. Nucleic Acids Res. 1999;27:1747-53 pubmed
    ..The two predominant proteins identified were: a Y-box protein (MSY2), the mammalian homolog to the Xenopus oocyte masking protein FRGY2/mRNP3+4, and a poly(A) binding protein...
  17. Medvedev S, Pan H, Schultz R. Absence of MSY2 in mouse oocytes perturbs oocyte growth and maturation, RNA stability, and the transcriptome. Biol Reprod. 2011;85:575-83 pubmed publisher
    ..b>MSY2, a germ cell-specific RNA-binding protein, is implicated in regulating mRNA stability...
  18. Yamaguchi S, Hong K, Liu R, Shen L, Inoue A, Diep D, et al. Tet1 controls meiosis by regulating meiotic gene expression. Nature. 2012;492:443-7 pubmed publisher
    ..Our study thus establishes a function for Tet1 in meiosis and meiotic gene activation in female germ cells. ..
  19. Schmidt D, Ovitt C, Anlag K, Fehsenfeld S, Gredsted L, Treier A, et al. The murine winged-helix transcription factor Foxl2 is required for granulosa cell differentiation and ovary maintenance. Development. 2004;131:933-42 pubmed
    ..In addition to providing a molecular mechanism for premature ovarian failure in BPES, these results suggest that granulosa cell function is not only crucial for oocyte growth but also to maintain follicular quiescence in vivo. ..