Gene Symbol: Vps35
Description: VPS35 retromer complex component
Alias: AI647796, Mem3, vacuolar protein sorting-associated protein 35, maternal embryonic message 3, maternal-embryonic 3, vacuolar protein sorting 35, vesicle protein sorting 35
Species: mouse
Products:     Vps35

Top Publications

  1. Belenkaya T, Wu Y, Tang X, Zhou B, Cheng L, Sharma Y, et al. The retromer complex influences Wnt secretion by recycling wntless from endosomes to the trans-Golgi network. Dev Cell. 2008;14:120-31 pubmed
    ..Here, we have examined a role of Vps35, an essential retromer subunit, in Wnt signaling in Drosophila and mammalian cells...
  2. Collins B, Skinner C, Watson P, Seaman M, Owen D. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Nat Struct Mol Biol. 2005;12:594-602 pubmed
  3. Harbour M, Breusegem S, Antrobus R, Freeman C, Reid E, Seaman M. The cargo-selective retromer complex is a recruiting hub for protein complexes that regulate endosomal tubule dynamics. J Cell Sci. 2010;123:3703-17 pubmed publisher
    ..two protein complexes that act together in endosome-to-Golgi retrieval; the cargo-selective complex is a trimer of VPS35, VPS29 and VPS26 that sorts cargo into tubules for retrieval to the Golgi...
  4. Wang C, Tang F, Peng Y, Shen C, Mei L, Xiong W. VPS35 regulates developing mouse hippocampal neuronal morphogenesis by promoting retrograde trafficking of BACE1. Biol Open. 2012;1:1248-57 pubmed publisher
    b>VPS35, a major component of the retromer, plays an important role in the selective endosome-to-Golgi retrieval of membrane proteins...
  5. Wen L, Tang F, Hong Y, Luo S, Wang C, He W, et al. VPS35 haploinsufficiency increases Alzheimer's disease neuropathology. J Cell Biol. 2011;195:765-79 pubmed publisher
    b>VPS35, a major component of the retromer complex, is important for endosome-to-Golgi retrieval of membrane proteins. Although implicated in Alzheimer's disease (AD), how VPS35 regulates AD-associated pathology is unknown...
  6. Xia W, Tang F, Xiong L, Xiong S, Jung J, Lee D, et al. Vps35 loss promotes hyperresorptive osteoclastogenesis and osteoporosis via sustained RANKL signaling. J Cell Biol. 2013;200:821-37 pubmed publisher
    ..We demonstrate in this paper that RANK trafficking, signaling, and function are regulated by VPS35, a major component of the retromer essential for selective endosome to Golgi retrieval of membrane proteins...
  7. Muhammad A, Flores I, Zhang H, Yu R, Staniszewski A, Planel E, et al. Retromer deficiency observed in Alzheimer's disease causes hippocampal dysfunction, neurodegeneration, and Abeta accumulation. Proc Natl Acad Sci U S A. 2008;105:7327-32 pubmed publisher
    ..By recapitulating features of the disease, these animal models suggest that retromer deficiency observed in late-onset Alzheimer's disease can contribute to disease pathogenesis. ..
  8. Kim E, Lee Y, Lee H, Kim J, Song B, Huh J, et al. Implication of mouse Vps26b-Vps29-Vps35 retromer complex in sortilin trafficking. Biochem Biophys Res Commun. 2010;403:167-71 pubmed publisher
    ..cargo recognition heterotrimer consisted of the vacuolar protein sorting (Vps) subunits Vps26, Vps29, and Vps35. In mammals, there are two different isoforms of Vps26, Vps26a and Vps26b, that localize to the endosome, and to ..
  9. MacLeod D, Rhinn H, Kuwahara T, Zolin A, Di Paolo G, McCabe B, et al. RAB7L1 interacts with LRRK2 to modify intraneuronal protein sorting and Parkinson's disease risk. Neuron. 2013;77:425-39 pubmed publisher
    ..defects in RAB7L1 or LRRK2 led to endolysosomal and Golgi apparatus sorting defects and deficiency of the VPS35 component of the retromer complex...

More Information


  1. Gokool S, Tattersall D, Reddy J, Seaman M. Identification of a conserved motif required for Vps35p/Vps26p interaction and assembly of the retromer complex. Biochem J. 2007;408:287-95 pubmed
    ..The recognition of cargo proteins by the retromer is performed by the Vps35p/VPS35 (where Vps is vacuolar protein sorting) component, which together with Vps26p/VPS26 and Vps29p/VPS29, forms the ..
  2. Kim E, Lee J, Baek D, Lee S, Kim M, Kim S, et al. Identification of novel retromer complexes in the mouse testis. Biochem Biophys Res Commun. 2008;375:16-21 pubmed publisher
    ..in mouse tissues were determined by Western blot and RT-PCR analyses, and the direct interaction of Vps26aT with Vps35 was also demonstrated by immunoprecipitation and pull-down assay using antibodies raised against each Vps ..
  3. Tian Y, Tang F, Sun X, Wen L, Mei L, Tang B, et al. VPS35-deficiency results in an impaired AMPA receptor trafficking and decreased dendritic spine maturation. Mol Brain. 2015;8:70 pubmed publisher
    b>Vacuolar protein sorting 35 (VPS35), a key component of retromer, plays an important role in endosome-to-Golgi retrieval of membrane proteins...
  4. Tang F, Erion J, Tian Y, Liu W, Yin D, Ye J, et al. VPS35 in Dopamine Neurons Is Required for Endosome-to-Golgi Retrieval of Lamp2a, a Receptor of Chaperone-Mediated Autophagy That Is Critical for α-Synuclein Degradation and Prevention of Pathogenesis of Parkinson's Disease. J Neurosci. 2015;35:10613-28 pubmed publisher
    Vacuolar protein sorting-35 (VPS35) is essential for endosome-to-Golgi retrieval of membrane proteins. Mutations in the VPS35 gene have been identified in patients with autosomal dominant PD...
  5. Temkin P, Morishita W, Goswami D, Arendt K, Chen L, Malenka R. The Retromer Supports AMPA Receptor Trafficking During LTP. Neuron. 2017;94:74-82.e5 pubmed publisher
    ..Using in vivo knockdown of the critical retromer component VPS35, we demonstrate a specific role for this endosomal sorting complex in the trafficking of AMPA receptors during ..
  6. Tang F, Liu W, Hu J, Erion J, Ye J, Mei L, et al. VPS35 Deficiency or Mutation Causes Dopaminergic Neuronal Loss by Impairing Mitochondrial Fusion and Function. Cell Rep. 2015;12:1631-43 pubmed publisher
    Vacuolar protein sorting-35 (VPS35) is a retromer component for endosomal trafficking. Mutations of VPS35 have been linked to familial Parkinson's disease (PD)...
  7. Hwang S, Benjamin L, Oh B, Rothstein J, Ackerman S, Beddington R, et al. Genetic mapping and embryonic expression of a novel, maternally transcribed gene Mem3. Mamm Genome. 1996;7:586-90 pubmed
    ..The genomic locus of Mem3 has been mapped to Chromosome (Chr) 8 near the D8Mit78 marker and the glutaryl CoA dehydrogenase (Gcdh) locus...
  8. Van Dyck F, Braem C, Chen Z, Declercq J, Deckers R, Kim B, et al. Loss of the PlagL2 transcription factor affects lacteal uptake of chylomicrons. Cell Metab. 2007;6:406-13 pubmed
    ..PlagL2 thus regulates important aspects of dietary lipid absorption, and the PlagL2(-/-) animal model has implications for the amelioration of obesity and the metabolic syndrome. ..
  9. Ishizu N, Yui D, Hebisawa A, Aizawa H, Cui W, Fujita Y, et al. Impaired striatal dopamine release in homozygous Vps35 D620N knock-in mice. Hum Mol Genet. 2016;25:4507-4517 pubmed publisher
    Point mutations in the vacuolar protein sorting 35 gene (VPS35) have been associated with an autosomal dominant form of late-onset Parkinson disease (PARK17), but there has been considerable debate over whether it is caused by a loss- or ..
  10. Dhungel N, Eleuteri S, Li L, Kramer N, Chartron J, Spencer B, et al. Parkinson's disease genes VPS35 and EIF4G1 interact genetically and converge on α-synuclein. Neuron. 2015;85:76-87 pubmed publisher
    ..Here we report an unexpected genetic interaction between two PD genes, VPS35 and EIF4G1. We provide evidence that EIF4G1 upregulation causes defects associated with protein misfolding...
  11. Liu W, Tang F, Lin S, Zhao K, Mei L, Ye J, et al. Vps35-deficiency impairs SLC4A11 trafficking and promotes corneal dystrophy. PLoS ONE. 2017;12:e0184906 pubmed publisher
    Vps35 (vacuolar protein sorting 35) is a major component of retromer that selectively promotes endosome-to-Golgi retrieval of transmembrane proteins...
  12. George A, Leahy H, Zhou J, Morin P. The vacuolar-ATPase inhibitor bafilomycin and mutant VPS35 inhibit canonical Wnt signaling. Neurobiol Dis. 2007;26:125-33 pubmed
    ..a possible indirect interaction between the LRP6 intracellular domain and vacuolar protein sorting protein 35 (VPS35)...
  13. Zhou G, Na S, Niedra R, Seed B. Deficits in receptor-mediated endocytosis and recycling in cells from mice with Gpr107 locus disruption. J Cell Sci. 2014;127:3916-27 pubmed publisher
    ..studies and proteomic analyses suggest that GPR107 associates with clathrin and the retromer protein VPS35 and that GPR107 might be responsible for the return of receptors to the plasma membrane from endocytic ..
  14. Liu W, Tang F, Erion J, Xiao H, Ye J, Xiong W. Vps35 haploinsufficiency results in degenerative-like deficit in mouse retinal ganglion neurons and impairment of optic nerve injury-induced gliosis. Mol Brain. 2014;7:10 pubmed publisher
    VPS35 (vacuolar protein sorting 35) is a major component of retromer that selectively promotes endosome-to-Golgi retrieval of transmembrane proteins...
  15. Wang C, Niu M, Zhou Z, Zheng X, Zhang L, Tian Y, et al. VPS35 regulates cell surface recycling and signaling of dopamine receptor D1. Neurobiol Aging. 2016;46:22-31 pubmed publisher
    b>Vacuolar protein sorting 35 (VPS35) is a retromer complex component regulating membrane protein trafficking and retrieval...
  16. Bartuzi P, Billadeau D, Favier R, Rong S, Dekker D, Fedoseienko A, et al. CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL. Nat Commun. 2016;7:10961 pubmed publisher
    ..Altogether, this study provides valuable insights into the mechanisms regulating cholesterol homeostasis and LDLR trafficking. ..
  17. Kerr M, Bennetts J, Simpson F, Thomas E, Flegg C, Gleeson P, et al. A novel mammalian retromer component, Vps26B. Traffic. 2005;6:991-1001 pubmed
    The mammalian retromer protein complex, which consists of three proteins--Vps26, Vps29, and Vps35--in association with members of the sorting nexin family of proteins, has been implicated in the trafficking of receptors and their ligands ..
  18. Chen C, Garcia Santos D, Ishikawa Y, Seguin A, Li L, Fegan K, et al. Snx3 regulates recycling of the transferrin receptor and iron assimilation. Cell Metab. 2013;17:343-52 pubmed publisher
    ..This impaired iron assimilation can be complemented with non-Tf iron chelates. We show that Snx3 and Vps35, a component of the retromer, interact with Tfrc to sort it to the recycling endosomes...
  19. Xiong L, Xia W, Tang F, Pan J, Mei L, Xiong W. Retromer in Osteoblasts Interacts With Protein Phosphatase 1 Regulator Subunit 14C, Terminates Parathyroid Hormone's Signaling, and Promotes Its Catabolic Response. EBioMedicine. 2016;9:45-60 pubmed publisher
    ..Here, we provide evidence for vacuolar protein sorting 35 (VPS35), a major component of retromer, in regulating PTH1R trafficking, turning off PTH signaling, and ..
  20. Zavodszky E, Seaman M, Moreau K, Jimenez Sanchez M, Breusegem S, Harbour M, et al. Mutation in VPS35 associated with Parkinson's disease impairs WASH complex association and inhibits autophagy. Nat Commun. 2014;5:3828 pubmed publisher
    ..b>VPS35 is a component of the retromer complex, which mediates endosome-to-Golgi retrieval of membrane proteins such as ..
  21. de Groot R, Farin H, Macůrková M, van Es J, Clevers H, Korswagen H. Retromer dependent recycling of the Wnt secretion factor Wls is dispensable for stem cell maintenance in the mammalian intestinal epithelium. PLoS ONE. 2013;8:e76971 pubmed publisher
    ..To answer this question, we generated a conditional Vps35 (fl) allele...
  22. Zhang P, Yu L, Gao J, Fu Q, Dai F, Zhao Y, et al. Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21. Genomics. 2000;70:253-7 pubmed
    ..The studies on yeast (Saccharomyces cerevisiae) VPS35, a hydrophilic membrane protein having a direct role in the retrieval of cargo proteins, suggest a mechanism ..
  23. Yin J, Liu X, He Q, Zhou L, Yuan Z, Zhao S. Vps35-dependent recycling of Trem2 regulates microglial function. Traffic. 2016;17:1286-1296 pubmed publisher
    ..is internalized in a clathrin-dependent manner and then recycled back to the plasma membrane through vacuolar protein sorting 35 (Vps35), the key component of cargo recognition core of retromer complex, but not Rab11...