Gene Symbol: Vcl
Description: vinculin
Alias: 9430097D22, AA571387, AI462105, AW545629, vinculin, metavinculin
Species: mouse
Products:     Vcl

Top Publications

  1. Klemm A, Diez G, Alonso J, Goldmann W. Comparing the mechanical influence of vinculin, focal adhesion kinase and p53 in mouse embryonic fibroblasts. Biochem Biophys Res Commun. 2009;379:799-801 pubmed publisher
    ..the intracellular course of action in mouse embryonic fibroblasts deficient in the focal adhesion proteins vinculin and focal adhesion kinase (FAK) and the nuclear matrix protein p53 using magnetic tweezer and nanoparticle ..
  2. Patel B, Gingras A, Bobkov A, Fujimoto L, Zhang M, Liddington R, et al. The activity of the vinculin binding sites in talin is influenced by the stability of the helical bundles that make up the talin rod. J Biol Chem. 2006;281:7458-67 pubmed
    The talin rod contains approximately 11 vinculin binding sites (VBSs), each defined by hydrophobic residues in a series of amphipathic helices that are normally buried within the helical bundles that make up the rod...
  3. Chen H, Cohen D, Choudhury D, Kioka N, Craig S. Spatial distribution and functional significance of activated vinculin in living cells. J Cell Biol. 2005;169:459-70 pubmed
    Conformational change is believed to be important to vinculin's function at sites of cell adhesion. However, nothing is known about vinculin's conformation in living cells...
  4. Kanchanawong P, Shtengel G, Pasapera A, Ramko E, Davidson M, Hess H, et al. Nanoscale architecture of integrin-based cell adhesions. Nature. 2010;468:580-4 pubmed publisher
    ..tails, focal adhesion kinase and paxillin; an intermediate force-transduction layer containing talin and vinculin; and an uppermost actin-regulatory layer containing zyxin, vasodilator-stimulated phosphoprotein and α-actinin...
  5. Diez G, Auernheimer V, Fabry B, Goldmann W. Head/tail interaction of vinculin influences cell mechanical behavior. Biochem Biophys Res Commun. 2011;406:85-8 pubmed publisher
    This study evaluates the influence of vinculin in closed conformation on the mechanical properties of cells...
  6. Dey T, Mann M, Goldmann W. Comparing mechano-transduction in fibroblasts deficient of focal adhesion proteins. Biochem Biophys Res Commun. 2011;413:541-4 pubmed publisher
    ..MEFs showed morphological changes and levels of apoptosis of the order: focal adhesion kinase>p130Cas>vinculin compared to wildtype cells...
  7. Mierke C, Kollmannsberger P, Zitterbart D, Smith J, Fabry B, Goldmann W. Mechano-coupling and regulation of contractility by the vinculin tail domain. Biophys J. 2008;94:661-70 pubmed
    b>Vinculin binds to multiple focal adhesion and cytoskeletal proteins and has been implicated in transmitting mechanical forces between the actin cytoskeleton and integrins or cadherins...
  8. Zemljic Harpf A, Miller J, Henderson S, Wright A, Manso A, Elsherif L, et al. Cardiac-myocyte-specific excision of the vinculin gene disrupts cellular junctions, causing sudden death or dilated cardiomyopathy. Mol Cell Biol. 2007;27:7522-37 pubmed
    b>Vinculin is a ubiquitously expressed multiliganded protein that links the actin cytoskeleton to the cell membrane. In myocytes, it is localized in protein complexes which anchor the contractile apparatus to the sarcolemma...
  9. Saunders R, Holt M, Jennings L, Sutton D, Barsukov I, Bobkov A, et al. Role of vinculin in regulating focal adhesion turnover. Eur J Cell Biol. 2006;85:487-500 pubmed
    Although vinculin (-/-) mouse embryo fibroblasts assemble focal adhesions (FAs), they spread more slowly, less extensively, and close a wound more rapidly than vinculin (+/+) cells...

More Information


  1. Humphries J, Wang P, Streuli C, Geiger B, Humphries M, Ballestrem C. Vinculin controls focal adhesion formation by direct interactions with talin and actin. J Cell Biol. 2007;179:1043-57 pubmed
    Focal adhesions (FAs) regulate cell migration. Vinculin, with its many potential binding partners, can interconnect signals in FAs...
  2. Subauste M, Pertz O, Adamson E, Turner C, Junger S, Hahn K. Vinculin modulation of paxillin-FAK interactions regulates ERK to control survival and motility. J Cell Biol. 2004;165:371-81 pubmed
    Cells lacking vinculin are highly metastatic and motile. The reasons for this finding have remained unclear. Both enhanced survival and motility are critical to metastasis...
  3. DeMali K, Barlow C, Burridge K. Recruitment of the Arp2/3 complex to vinculin: coupling membrane protrusion to matrix adhesion. J Cell Biol. 2002;159:881-91 pubmed signals that trigger membrane protrusion, the actin-related protein (Arp)2/3 complex transiently binds to vinculin, an integrin-associated protein...
  4. Conti F, Monkley S, Wood M, Critchley D, Muller U. Talin 1 and 2 are required for myoblast fusion, sarcomere assembly and the maintenance of myotendinous junctions. Development. 2009;136:3597-606 pubmed publisher
    ..We conclude that talin 1 and 2 are crucial for skeletal muscle development, where they regulate myoblast fusion, sarcomere assembly and the maintenance of MTJs. ..
  5. Carisey A, Tsang R, Greiner A, Nijenhuis N, Heath N, Nazgiewicz A, et al. Vinculin regulates the recruitment and release of core focal adhesion proteins in a force-dependent manner. Curr Biol. 2013;23:271-81 pubmed publisher
    ..The molecular basis of how these sensing units are regulated, how they are implicated in transducing mechanical stimuli, and how this leads to a spatiotemporal coordination of FAs is unclear...
  6. Huttelmaier S, Illenberger S, Grosheva I, Rudiger M, Singer R, Jockusch B. Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins. J Cell Biol. 2001;155:775-86 pubmed
    By screening a yeast two-hybrid library with COOH-terminal fragments of vinculin/metavinculin as the bait, we identified a new protein termed raver1...
  7. Gertler F, Niebuhr K, Reinhard M, Wehland J, Soriano P. Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics. Cell. 1996;87:227-39 pubmed
    ..Expression of neural-enriched isoforms of Mena in fibroblasts induces the formation of abnormal F-actin-rich outgrowths, supporting a role for this protein in microfilament assembly and cell motility...
  8. Pasapera A, Schneider I, Rericha E, Schlaepfer D, Waterman C. Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation. J Cell Biol. 2010;188:877-90 pubmed publisher
    ..We find that FA recruitment of both the cytoskeletal adapter protein vinculin and the tyrosine kinase FA kinase (FAK) are myosin II and extracellular matrix (ECM) stiffness dependent...
  9. Gan B, Peng X, Nagy T, Alcaraz A, Gu H, Guan J. Role of FIP200 in cardiac and liver development and its regulation of TNFalpha and TSC-mTOR signaling pathways. J Cell Biol. 2006;175:121-33 pubmed
    ..Together, our results reveal that FIP200 functions as a regulatory node to couple two important signaling pathways to regulate cell growth and survival during mouse embryogenesis. ..
  10. Coll J, Ben Ze ev A, Ezzell R, Rodríguez Fernandez J, Baribault H, Oshima R, et al. Targeted disruption of vinculin genes in F9 and embryonic stem cells changes cell morphology, adhesion, and locomotion. Proc Natl Acad Sci U S A. 1995;92:9161-5 pubmed
    b>Vinculin, a major constituent of focal adhesions and zonula adherens junctions, is thought to be involved in linking the microfilaments to areas of cell-substrate and cell-cell contacts...
  11. Diez G, Kollmannsberger P, Mierke C, Koch T, Vali H, Fabry B, et al. Anchorage of vinculin to lipid membranes influences cell mechanical properties. Biophys J. 2009;97:3105-12 pubmed publisher
    The focal adhesion protein vinculin (1066 residues) can be separated into a 95-kDa head and a 30-kDa tail domain...
  12. Mierke C, Kollmannsberger P, Zitterbart D, Diez G, Koch T, Marg S, et al. Vinculin facilitates cell invasion into three-dimensional collagen matrices. J Biol Chem. 2010;285:13121-30 pubmed publisher
    The cytoskeletal protein vinculin contributes to the mechanical link of the contractile actomyosin cytoskeleton to the extracellular matrix (ECM) through integrin receptors...
  13. Xu W, Baribault H, Adamson E. Vinculin knockout results in heart and brain defects during embryonic development. Development. 1998;125:327-37 pubmed
    The vinculin gene codes for a cytoskeletal protein, found in focal adhesion plaques and in cell-cell adherens junctions. Vinculin was inactivated by homologous recombination using a targeting vector in embryonic stem (ES) cells...
  14. Zemljic Harpf A, Ponrartana S, Avalos R, Jordan M, Roos K, Dalton N, et al. Heterozygous inactivation of the vinculin gene predisposes to stress-induced cardiomyopathy. Am J Pathol. 2004;165:1033-44 pubmed
    Vinculin and its muscle splice variant metavinculin link focal adhesions and cell-to-cell contact sites to the actin cytoskeleton...
  15. Witt S, Zieseniss A, Fock U, Jockusch B, Illenberger S. Comparative biochemical analysis suggests that vinculin and metavinculin cooperate in muscular adhesion sites. J Biol Chem. 2004;279:31533-43 pubmed
    b>Metavinculin, the muscle-specific splice variant of the cell adhesion protein vinculin, is characterized by a 68-amino acid insert within the C-terminal tail domain...
  16. Mandai K, Nakanishi H, Satoh A, Takahashi K, Satoh K, Nishioka H, et al. Ponsin/SH3P12: an l-afadin- and vinculin-binding protein localized at cell-cell and cell-matrix adherens junctions. J Cell Biol. 1999;144:1001-17 pubmed
    ..Ponsin furthermore directly bound vinculin, an F-actin-binding protein localized at ZA in epithelial cells, at cell-cell AJ in nonepithelial cells, and at ..
  17. Liang X, Sun Y, Ye M, Scimia M, Cheng H, Martin J, et al. Targeted ablation of PINCH1 and PINCH2 from murine myocardium results in dilated cardiomyopathy and early postnatal lethality. Circulation. 2009;120:568-76 pubmed publisher
    ..These results demonstrate essential roles for PINCHs in myocardial growth, maturation, remodeling, and function and highlight the importance of studying the role of PINCHs in human cardiac injury and cardiomyopathy. ..
  18. Goldmann W, Ingber D. Intact vinculin protein is required for control of cell shape, cell mechanics, and rac-dependent lamellipodia formation. Biochem Biophys Res Commun. 2002;290:749-55 pubmed
    Studies were carried out using vinculin-deficient F9 embryonic carcinoma (gamma229) cells to analyze the relationship between structure and function within the focal adhesion protein vinculin, in the context of control of cell shape, cell ..
  19. Gingras A, Bate N, Goult B, Patel B, Kopp P, Emsley J, et al. Central region of talin has a unique fold that binds vinculin and actin. J Biol Chem. 2010;285:29577-87 pubmed publisher
    ..8) is higher than other rod domains. Both helical bundles contain vinculin-binding sites but that in the isolated 5-helix bundle is cryptic, whereas that in the isolated 4-helix bundle is ..
  20. Bois P, O Hara B, Nietlispach D, Kirkpatrick J, Izard T. The vinculin binding sites of talin and alpha-actinin are sufficient to activate vinculin. J Biol Chem. 2006;281:7228-36 pubmed
    b>Vinculin regulates both cell-cell and cell-matrix junctions and anchors adhesion complexes to the actin cytoskeleton through its interactions with the vinculin binding sites of alpha-actinin or talin...
  21. Menko A, Zhang L, Schiano F, Kreidberg J, Kukuruzinska M. Regulation of cadherin junctions during mouse submandibular gland development. Dev Dyn. 2002;224:321-33 pubmed
  22. Cattin M, Wang J, Weldrick J, Roeske C, Mak E, Thorn S, et al. Deletion of MLIP (muscle-enriched A-type lamin-interacting protein) leads to cardiac hyperactivation of Akt/mammalian target of rapamycin (mTOR) and impaired cardiac adaptation. J Biol Chem. 2015;290:26699-714 pubmed publisher
    ..Together, these results revealed that MLIP is required for normal myocardial adaptation to stress through integrated regulation of the Akt/mTOR pathways. ..
  23. Digman M, Wiseman P, Choi C, Horwitz A, Gratton E. Stoichiometry of molecular complexes at adhesions in living cells. Proc Natl Acad Sci U S A. 2009;106:2170-5 pubmed publisher
    ..We apply the cross-variance method to determine the stoichiometry of complexes containing paxillin and vinculin or focal adhesion kinase (FAK) in disassembling adhesions in mouse embryo fibroblasts expressing FAK, vinculin, ..
  24. Ohmori T, Kashiwakura Y, Ishiwata A, Madoiwa S, Mimuro J, Furukawa Y, et al. Vinculin is indispensable for repopulation by hematopoietic stem cells, independent of integrin function. J Biol Chem. 2010;285:31763-73 pubmed publisher
    b>Vinculin is a highly conserved actin-binding protein that is localized in integrin-mediated focal adhesion complexes...
  25. Ben Ze ev A, Reiss R, Bendori R, Gorodecki B. Transient induction of vinculin gene expression in 3T3 fibroblasts stimulated by serum-growth factors. Cell Regul. 1990;1:621-36 pubmed
    When stimulated with serum, quiescent Balb/C-3T3 fibroblasts were found to induce vinculin transcription transiently within 30 min, followed by accumulation of vinculin mRNA and protein synthesis between 2 and 4 h after stimulation and a ..
  26. Balasubramanian S, Quinones L, Kasiganesan H, Zhang Y, Pleasant D, Sundararaj K, et al. ?3 integrin in cardiac fibroblast is critical for extracellular matrix accumulation during pressure overload hypertrophy in mouse. PLoS ONE. 2012;7:e45076 pubmed publisher
    ..These results indicate that ?3 integrin-mediated Pyk2 signaling in cardiac fibroblasts plays a critical role in PO-induced cardiac fibrosis. ..
  27. Atherton P, Stutchbury B, Wang D, Jethwa D, Tsang R, Meiler Rodriguez E, et al. Vinculin controls talin engagement with the actomyosin machinery. Nat Commun. 2015;6:10038 pubmed publisher
    ..Here, we demonstrate how the actin-binding proteins talin and vinculin cooperate to provide this link...
  28. Hoffmeister M, Krieg J, Ehrke A, Seigfried F, Wischmann L, Dietmann P, et al. Developmental neurogenesis in mouse and Xenopus is impaired in the absence of Nosip. Dev Biol. 2017;429:200-212 pubmed publisher
    ..Nosip is a novel factor critical for neural stem cell/progenitor self-renewal and neurogenesis during mouse and Xenopus development and functions upstream of Rbp1 during early neurogenesis. ..
  29. Weller P, Ogryzko E, Corben E, Zhidkova N, Patel B, Price G, et al. Complete sequence of human vinculin and assignment of the gene to chromosome 10. Proc Natl Acad Sci U S A. 1990;87:5667-71 pubmed
    We have determined the complete sequence of human vinculin, a cytoskeletal protein associated with cell-cell and cell-matrix junctions...
  30. Tanoue T, Takeichi M. Mammalian Fat1 cadherin regulates actin dynamics and cell-cell contact. J Cell Biol. 2004;165:517-28 pubmed
    ..These results suggest that Fat1 regulates actin cytoskeletal organization at cell peripheries, thereby modulating cell contacts and polarity. ..
  31. Ishiyama N, Tanaka N, Abe K, Yang Y, Abbas Y, Umitsu M, et al. An autoinhibited structure of ?-catenin and its implications for vinculin recruitment to adherens junctions. J Biol Chem. 2013;288:15913-25 pubmed publisher
    Catenin is an actin- and vinculin-binding protein that regulates cell-cell adhesion by interacting with cadherin adhesion receptors through ?-catenin, but the mechanisms by which it anchors the cadherin-catenin complex to the actin ..
  32. Auernheimer V, Goldmann W. Serine phosphorylation on position 1033 of vinculin impacts cellular mechanics. Biochem Biophys Res Commun. 2014;450:1095-8 pubmed publisher
    This study evaluates the influence of S1033 vinculin phosphorylation on the mechanical properties of cells...
  33. Schwartz B, Marks M, Wittler L, Werber M, Währisch S, Nordheim A, et al. SRF is essential for mesodermal cell migration during elongation of the embryonic body axis. Mech Dev. 2014;133:23-35 pubmed publisher
    ..Thus, the primary role for SRF in the nascent mesoderm during elongation of the embryonic body axis is the activation of a migratory program, which is a prerequisite for axis extension. ..
  34. Chen J, Murchison E, Tang R, Callis T, Tatsuguchi M, Deng Z, et al. Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart failure. Proc Natl Acad Sci U S A. 2008;105:2111-6 pubmed publisher
    ..Together, our studies demonstrate essential roles for Dicer in cardiac contraction and indicate that miRNAs play critical roles in normal cardiac function and under pathological conditions. ..
  35. Holtz M, Misra R. Serum response factor is required for cell contact maintenance but dispensable for proliferation in visceral yolk sac endothelium. BMC Dev Biol. 2011;11:18 pubmed publisher
    ..adhesion (FA) integrins alpha5 and beta1 were largely unchanged in contrast to loss of the FA-associated molecule vinculin. The integrin binding partner fibronectin-1 was also profoundly decreased in the extracellular matrix, indicating ..
  36. Nagasato A, Yamashita H, Matsuo M, Ueda K, Kioka N. The distribution of vinculin to lipid rafts plays an important role in sensing stiffness of extracellular matrix. Biosci Biotechnol Biochem. 2017;81:1136-1147 pubmed publisher
    ..Our previous study has shown that the interaction of the focal adhesion protein vinculin with vinexin ? plays a critical role in sensing ECM stiffness and regulating stiffness-dependent cell migration...
  37. Kamiya K, Yum S, Kurebayashi N, Muraki M, Ogawa K, Karasawa K, et al. Assembly of the cochlear gap junction macromolecular complex requires connexin 26. J Clin Invest. 2014;124:1598-607 pubmed publisher
    ..Our results demonstrate that deafness-associated mutations in CX26 induce the macromolecular degradation of large gap junction complexes accompanied by an increase in caveolar structures. ..
  38. Moik D, Janbandhu V, Fassler R. Loss of migfilin expression has no overt consequences on murine development and homeostasis. J Cell Sci. 2011;124:414-21 pubmed publisher
    ..Our findings indicate that the roles of migfilin are functionally redundant during mouse development and tissue homeostasis. ..
  39. Liu F, Lee J, Wei H, Tanabe O, Engel J, Morrison S, et al. FIP200 is required for the cell-autonomous maintenance of fetal hematopoietic stem cells. Blood. 2010;116:4806-14 pubmed publisher
    ..These data identify FIP200 as a key intrinsic regulator of fetal HSCs and implicate a potential role for autophagy in the maintenance of fetal hematopoiesis and HSCs. ..
  40. Lien W, Gelfand V, Vasioukhin V. Alpha-E-catenin binds to dynamitin and regulates dynactin-mediated intracellular traffic. J Cell Biol. 2008;183:989-97 pubmed publisher
  41. Sents W, Meeusen B, Kalev P, Radaelli E, Sagaert X, Miermans E, et al. PP2A Inactivation Mediated by PPP2R4 Haploinsufficiency Promotes Cancer Development. Cancer Res. 2017;77:6825-6837 pubmed publisher
    ..Our results provide evidence for PPP2R4 as a haploinsufficient tumor suppressor gene, defining a high-penetrance genetic mechanism for PP2A inhibition in human cancer. Cancer Res; 77(24); 6825-37. ©2017 AACR. ..
  42. Park H, Lee J, Gouin E, Cossart P, Izard T. The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin. J Biol Chem. 2011;286:35096-103 pubmed publisher
    ..Here we report that the cell surface antigen sca4 of Rickettsia co-localizes with vinculin in cells at sites of focal adhesions in sca4-transfected cells and that sca4 binds to and activates vinculin ..
  43. Pashmforoush M, Pomies P, Peterson K, Kubalak S, Ross J, Hefti A, et al. Adult mice deficient in actinin-associated LIM-domain protein reveal a developmental pathway for right ventricular cardiomyopathy. Nat Med. 2001;7:591-7 pubmed
    ..Taken together, these studies point to a novel developmental pathway for RV dilated cardiomyopathy via instability of alpha-actinin complexes. ..
  44. Sanger J, Wang J, Gleason L, Chowrashi P, Dube D, Mittal B, et al. Arg/Abl-binding protein, a Z-body and Z-band protein, binds sarcomeric, costameric, and signaling molecules. Cytoskeleton (Hoboken). 2010;67:808-23 pubmed publisher
    ..GST-ArgBP2-SH3 bound actin, ?-actinin and vinculin proteins in blot overlays, cosedimentation assays, and EM negative staining techniques...
  45. Subauste M, Nalbant P, Adamson E, Hahn K. Vinculin controls PTEN protein level by maintaining the interaction of the adherens junction protein beta-catenin with the scaffolding protein MAGI-2. J Biol Chem. 2005;280:5676-81 pubmed
    ..Cancer cells frequently exhibit loss of cadherin, beta-catenin, alpha-catenin and/or vinculin, key elements of adherens junctions...
  46. Peng X, Wu X, Druso J, Wei H, Park A, Kraus M, et al. Cardiac developmental defects and eccentric right ventricular hypertrophy in cardiomyocyte focal adhesion kinase (FAK) conditional knockout mice. Proc Natl Acad Sci U S A. 2008;105:6638-43 pubmed publisher
    ..Last, we found that Src, but not PI3K, is important in mediating signal transduction for the regulation of MEF2a by FAK. Together, these results identified the role and mechanisms of FAK in embryonic cardiac development. ..
  47. Thomas W, Boscher C, Chu Y, Cuvelier D, Martinez Rico C, Seddiki R, et al. ?-Catenin and vinculin cooperate to promote high E-cadherin-based adhesion strength. J Biol Chem. 2013;288:4957-69 pubmed publisher
    ..The cadherin cytoplasmic domain recruits several partners, including catenins and vinculin, at sites of cell-cell adhesion...
  48. Plotnikov S, Pasapera A, Sabass B, Waterman C. Force fluctuations within focal adhesions mediate ECM-rigidity sensing to guide directed cell migration. Cell. 2012;151:1513-27 pubmed publisher
    ..We show that a FAK/phosphopaxillin/vinculin pathway is essential for high FA traction and to enable tugging FA traction over a broad range of ECM rigidities...
  49. Auernheimer V, Lautscham L, Leidenberger M, Friedrich O, Kappes B, Fabry B, et al. Vinculin phosphorylation at residues Y100 and Y1065 is required for cellular force transmission. J Cell Sci. 2015;128:3435-43 pubmed publisher
    The focal adhesion protein vinculin connects the actin cytoskeleton, through talin and integrins, with the extracellular matrix...
  50. Dumbauld D, Shin H, Gallant N, Michael K, Radhakrishna H, Garcia A. Contractility modulates cell adhesion strengthening through focal adhesion kinase and assembly of vinculin-containing focal adhesions. J Cell Physiol. 2010;223:746-56 pubmed publisher
    ..Reductions in adhesion strengthening by inhibitors of contractility correlated with loss of vinculin and talin from focal adhesions without changes in integrin binding...
  51. Rout U, Wang J, Paria B, Armant D. Alpha5beta1, alphaVbeta3 and the platelet-associated integrin alphaIIbbeta3 coordinately regulate adhesion and migration of differentiating mouse trophoblast cells. Dev Biol. 2004;268:135-51 pubmed
    ..These data demonstrate that trafficking of alphaIIb regulates adhesion between trophoblast cells and fibronectin as invasion of the endometrium commences. ..
  52. Migliorini D, Bogaerts S, Defever D, Vyas R, Denecker G, Radaelli E, et al. Cop1 constitutively regulates c-Jun protein stability and functions as a tumor suppressor in mice. J Clin Invest. 2011;121:1329-43 pubmed publisher
    ..In the absence of evidence for a genetic interaction between Cop1 and p53, our data strongly argue against the use of Cop1-inhibitory drugs for cancer therapy. ..
  53. Thompson P, Ramachandran S, Case L, Tolbert C, Tandon A, Pershad M, et al. A Structural Model for Vinculin Insertion into PIP2-Containing Membranes and the Effect of Insertion on Vinculin Activation and Localization. Structure. 2017;25:264-275 pubmed publisher
    b>Vinculin, a scaffolding protein that localizes to focal adhesions (FAs) and adherens junctions, links the actin cytoskeleton to the adhesive super-structure...
  54. Hölscher M, Silter M, Krull S, von Ahlen M, Hesse A, Schwartz P, et al. Cardiomyocyte-specific prolyl-4-hydroxylase domain 2 knock out protects from acute myocardial ischemic injury. J Biol Chem. 2011;286:11185-94 pubmed publisher
    ..This correlated with a decreased number of apoptotic cells in the infarcted myocardium in the cPhd2(-/-) mice and significantly improved cardiac function 3 weeks after myocardial infarction. ..
  55. Hoffmeister M, Prelle C, Küchler P, Kovacevic I, Moser M, Müller Esterl W, et al. The ubiquitin E3 ligase NOSIP modulates protein phosphatase 2A activity in craniofacial development. PLoS ONE. 2014;9:e116150 pubmed publisher
    ..We conclude, that NOSIP is a critical modulator of brain and craniofacial development in mice and a candidate gene for holoprosencephaly in humans. ..
  56. Sinn H, Balsamo J, Lilien J, Lin J. Localization of the novel Xin protein to the adherens junction complex in cardiac and skeletal muscle during development. Dev Dyn. 2002;225:1-13 pubmed
    ..Xin is detected earlier than vinculin in the developing heart and colocalizes with vinculin at the intercalated disc but not at the sarcolemma within ..
  57. Babic I, Sharma S, Black D. A role for polypyrimidine tract binding protein in the establishment of focal adhesions. Mol Cell Biol. 2009;29:5564-77 pubmed publisher
    ..This cytoplasmic PTB is associated with transcripts encoding the focal adhesion scaffolding proteins vinculin and alpha-actinin 4...
  58. van der Flier A, Kuikman I, Kramer D, Geerts D, Kreft M, Takafuta T, et al. Different splice variants of filamin-B affect myogenesis, subcellular distribution, and determine binding to integrin [beta] subunits. J Cell Biol. 2002;156:361-76 pubmed
    ..These findings suggest that specific combinations of filamin mRNA splicing events modulate the organization of the actin cytoskeleton and the binding affinity for integrins. ..
  59. Wang C, Liang C, Bian Z, Zhu Y, Guan J. FIP200 is required for maintenance and differentiation of postnatal neural stem cells. Nat Neurosci. 2013;16:532-42 pubmed publisher
    ..These data reveal that FIP200-mediated autophagy contributes to the maintenance and functions of NSCs through regulation of oxidative state. ..
  60. Hayashi H, Sone M, Ito S, Wakamatsu K, Kato M, Nakashima I, et al. A novel RFP-RET transgenic mouse model with abundant eumelanin in the cochlea. Hear Res. 2004;195:35-40 pubmed
    ..In addition, we provide evidence that eumelanin overproduction in the cochlea does not affect normal hearing. ..
  61. Tolbert C, Thompson P, Superfine R, Burridge K, Campbell S. Phosphorylation at Y1065 in vinculin mediates actin bundling, cell spreading, and mechanical responses to force. Biochemistry. 2014;53:5526-36 pubmed publisher
    b>Vinculin is an essential structural adaptor protein that localizes to sites of adhesion and is involved in a number of cell processes including adhesion, spreading, motility, force transduction, and cell survival...
  62. Kuroda M, Wada H, Kimura Y, Ueda K, Kioka N. Vinculin promotes nuclear localization of TAZ to inhibit ECM stiffness-dependent differentiation into adipocytes. J Cell Sci. 2017;130:989-1002 pubmed publisher
    ..In this study, we show that the cytoskeletal focal adhesion protein vinculin plays a critical role in the ECM stiffness-dependent adipocyte differentiation of MSCs...
  63. Dong Y, Zhang L, Zhang S, Bai Y, Chen H, Sun X, et al. Phosphatase of regenerating liver 2 (PRL2) is essential for placental development by down-regulating PTEN (Phosphatase and Tensin Homologue Deleted on Chromosome 10) and activating Akt protein. J Biol Chem. 2012;287:32172-9 pubmed publisher
    ..This study provides the first evidence that PRL2 is required for extra-embryonic development and associates the oncogenic properties of PRL2 with its ability to negatively regulate PTEN, thereby activating the PI3K-Akt pathway. ..
  64. Fukunaga T, Zou W, Warren J, Teitelbaum S. Vinculin regulates osteoclast function. J Biol Chem. 2014;289:13554-64 pubmed publisher
    Osteoclastic bone resorption depends upon the cell's ability to organize its cytoskeleton. Because vinculin (VCL) is an actin-binding protein, we asked whether it participates in skeletal degradation...
  65. Greiner T, Kesavan G, Ståhlberg A, Semb H. Rac1 regulates pancreatic islet morphogenesis. BMC Dev Biol. 2009;9:2 pubmed publisher
    ..Our results further emphasize Rac1 as a key regulator of cell migration and cell adhesion during tissue and organ morphogenesis. ..
  66. Seto J, Lek M, Quinlan K, Houweling P, Zheng X, Garton F, et al. Deficiency of ?-actinin-3 is associated with increased susceptibility to contraction-induced damage and skeletal muscle remodeling. Hum Mol Genet. 2011;20:2914-27 pubmed publisher
    ..In support of this, we demonstrated that the Z-disk proteins, ZASP, titin and vinculin preferentially bind to ?-actinin-2...
  67. Gavard J, Lambert M, Grosheva I, Marthiens V, Irinopoulou T, Riou J, et al. Lamellipodium extension and cadherin adhesion: two cell responses to cadherin activation relying on distinct signalling pathways. J Cell Sci. 2004;117:257-70 pubmed
    ..These results provide additional information on the mechanisms by which cadherin coordinates adhesion with dynamic changes in the cytoskeleton to control cell shape and intercellular junction organization. ..
  68. Halstead J, Savaskan N, van den Bout I, van Horck F, Hajdo Milasinovic A, Snell M, et al. Rac controls PIP5K localisation and PtdIns(4,5)P? synthesis, which modulates vinculin localisation and neurite dynamics. J Cell Sci. 2010;123:3535-46 pubmed publisher
    ..To clarify how increased levels of PtdIns(4,5)P? induce neurite retraction, we show that mutants of vinculin that are unable to interact with PtdIns(4,5)P?, attenuate PIP5K- and LPA-induced neurite retraction...
  69. Gallant N, Michael K, Garcia A. Cell adhesion strengthening: contributions of adhesive area, integrin binding, and focal adhesion assembly. Mol Biol Cell. 2005;16:4329-40 pubmed
    ..Adhesive area strongly modulated adhesion strength, integrin binding, and vinculin and talin recruitment, exhibiting linear increases for small areas...
  70. Bennett P, Maggs A, Baines A, Pinder J. The transitional junction: a new functional subcellular domain at the intercalated disc. Mol Biol Cell. 2006;17:2091-100 pubmed
    ..In particular, it provides a means for sarcomeres to be added to the ends of the cells during growth. This is of particular relevance to understanding myocyte elongation in dilated cardiomyopathy. ..
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    b>Vinculin (Vcl) plays a key structural role in ventricular myocytes that, when disrupted, can lead to contractile dysfunction and dilated cardiomyopathy...
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    ..Given these results, we conclude that Tln1 and Tln2 have distinct functions in the myocardium. Our data show that reduction of CM Tln1 expression can lead to improved cardiac remodeling following pressure overload. ..
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    ..However, the number of Tln2(cd/cd) pups surviving to adulthood was variable suggesting that such mice have an underlying defect. ..
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    ..Finally, we demonstrate that PINCH2 localizes to focal adhesions in NIH 3T3 cells and to Z-disks in primary rat cardiomyocytes. ..
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    Summary.? Vinculin links integrins to the cell cytoskeleton by virtue of its binding to proteins such as talin and F-actin...
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