Gene Symbol: Ubtf
Description: upstream binding transcription factor, RNA polymerase I
Alias: A930005G04Rik, NOR-90, Tcfubf, UBF, UBF-1, UBF1, nucleolar transcription factor 1, transcription factor UBF, upstream-binding factor 1
Species: mouse
Products:     Ubtf

Top Publications

  1. Poortinga G, Hannan K, Snelling H, Walkley C, Jenkins A, Sharkey K, et al. MAD1 and c-MYC regulate UBF and rDNA transcription during granulocyte differentiation. EMBO J. 2004;23:3325-35 pubmed
    ..by MAD1 was associated with its ability to interact directly with the promoter of upstream binding factor (UBF), an rDNA regulatory factor. Conversely, c-MYC activated transcription from the UBF promoter...
  2. Hannan K, Brandenburger Y, Jenkins A, Sharkey K, Cavanaugh A, Rothblum L, et al. mTOR-dependent regulation of ribosomal gene transcription requires S6K1 and is mediated by phosphorylation of the carboxy-terminal activation domain of the nucleolar transcription factor UBF. Mol Cell Biol. 2003;23:8862-77 pubmed
    ..led to a rapid dephosphorylation of the carboxy-terminal activation domain of the rDNA transcription factor, UBF, which significantly reduced its ability to associate with the basal rDNA transcription factor SL-1...
  3. Sanij E, Poortinga G, Sharkey K, Hung S, Holloway T, Quin J, et al. UBF levels determine the number of active ribosomal RNA genes in mammals. J Cell Biol. 2008;183:1259-74 pubmed publisher
    ..by the mutation of an extracellular signal-regulated kinase site within the high mobility group box 1 domain of UBF1, which is required for its ability to bend and loop DNA in vitro...
  4. Stefanovsky V, Moss T. The splice variants of UBF differentially regulate RNA polymerase I transcription elongation in response to ERK phosphorylation. Nucleic Acids Res. 2008;36:5093-101 pubmed publisher
    The mammalian architectural HMGB-Box transcription factor UBF is ubiquitously expressed in two variant forms as the result of a differential splicing event, that in the UBF2 deletes 37 amino acid from the second of six HMGB-boxes...
  5. Meraner J, Lechner M, Loidl A, Goralik Schramel M, Voit R, Grummt I, et al. Acetylation of UBF changes during the cell cycle and regulates the interaction of UBF with RNA polymerase I. Nucleic Acids Res. 2006;34:1798-806 pubmed
    The upstream binding factor UBF, an activator of RNA polymerase I transcription, is posttranslationally modified by phosphorylation and acetylation. We found that in NIH3T3 cells, UBF is acetylated in S-phase but not in G1-phase...
  6. Yuan X, Zhou Y, Casanova E, Chai M, Kiss E, Grone H, et al. Genetic inactivation of the transcription factor TIF-IA leads to nucleolar disruption, cell cycle arrest, and p53-mediated apoptosis. Mol Cell. 2005;19:77-87 pubmed
    ..The striking correlation between perturbation of nucleolar function, elevated levels of p53, and induction of cell suicide supports the view that the nucleolus is a stress sensor that regulates p53 activity. ..
  7. Nagai K. Molecular evolution of Sry and Sox gene. Gene. 2001;270:161-9 pubmed
    ..Subsequent divergences include the nucleolar UBF, nuclear SSRP as well as fungal mating protein Mc, MAT and Ste11...
  8. Lee J, Hwang Y, Boo J, Han D, Kwon O, Todorova K, et al. Dysregulation of upstream binding factor-1 acetylation at K352 is linked to impaired ribosomal DNA transcription in Huntington's disease. Cell Death Differ. 2011;18:1726-35 pubmed publisher
    ..We identified that UBF1 acetylation at Lys (K) 352 by CREB binding protein (CBP) is crucial for the transcriptional activity of rDNA...
  9. Herdman C, Mars J, Stefanovsky V, Tremblay M, Sabourin Felix M, Lindsay H, et al. A unique enhancer boundary complex on the mouse ribosomal RNA genes persists after loss of Rrn3 or UBF and the inactivation of RNA polymerase I transcription. PLoS Genet. 2017;13:e1006899 pubmed publisher
    ..basal transcription factors, show that preinitiation complex formation is driven by the architectural factor UBF (UBTF) independently of transcription, and that RPI termination and release corresponds with the site of TTF1 binding...

More Information


  1. Sanij E, Diesch J, Lesmana A, Poortinga G, Hein N, Lidgerwood G, et al. A novel role for the Pol I transcription factor UBTF in maintaining genome stability through the regulation of highly transcribed Pol II genes. Genome Res. 2015;25:201-12 pubmed publisher
    ..Upstream binding transcription factor (UBTF, also called UBF) is thought to function exclusively in RNA polymerase I (Pol I)-specific transcription of the ..
  2. Guetg C, Lienemann P, Sirri V, Grummt I, Hernandez Verdun D, Hottiger M, et al. The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats. EMBO J. 2010;29:2135-46 pubmed publisher
    ..These findings point to a role of TIP5 in protecting genome stability and suggest that it can play a role in the cellular transformation process. ..
  3. Mekhail K, Khacho M, Carrigan A, Hache R, Gunaratnam L, Lee S. Regulation of ubiquitin ligase dynamics by the nucleolus. J Cell Biol. 2005;170:733-44 pubmed
    ..Data shown here provide the first evidence that cells have evolved a mechanism to regulate molecular networks by reversibly switching proteins between a mobile and static state. ..
  4. Prisco M, Maiorana A, Guerzoni C, Calin G, Calabretta B, Voit R, et al. Role of pescadillo and upstream binding factor in the proliferation and differentiation of murine myeloid cells. Mol Cell Biol. 2004;24:5421-33 pubmed
    Pescadillo (PES1) and the upstream binding factor (UBF1) play a role in ribosome biogenesis, which regulates cell size, an important component of cell proliferation...
  5. Zhang S, Köhler C, Hemmerich P, Grosse F. Nuclear DNA helicase II (RNA helicase A) binds to an F-actin containing shell that surrounds the nucleolus. Exp Cell Res. 2004;293:248-58 pubmed
    ..05 microg/ml actinomycin D, while nucleolin and the upstream binding factor (UBF) remained there...
  6. Hamdane N, Herdman C, Mars J, Stefanovsky V, Tremblay M, Moss T. Depletion of the cisplatin targeted HMGB-box factor UBF selectively induces p53-independent apoptotic death in transformed cells. Oncotarget. 2015;6:27519-36 pubmed publisher
    Cisplatin-DNA adducts act as strong decoys for the Upstream Binding Factor UBF (UBTF) and have been shown to inhibit transcription of the ribosomal RNA genes by RNA polymerase I...
  7. Hamdane N, Stefanovsky V, Tremblay M, Németh A, Paquet E, Lessard F, et al. Conditional inactivation of Upstream Binding Factor reveals its epigenetic functions and the existence of a somatic nucleolar precursor body. PLoS Genet. 2014;10:e1004505 pubmed publisher
    Upstream Binding Factor (UBF) is a unique multi-HMGB-box protein first identified as a co-factor in RNA polymerase I (RPI/PolI) transcription...
  8. Mariappan M, D Silva K, Lee M, Sataranatarajan K, Barnes J, Choudhury G, et al. Ribosomal biogenesis induction by high glucose requires activation of upstream binding factor in kidney glomerular epithelial cells. Am J Physiol Renal Physiol. 2011;300:F219-30 pubmed publisher
    ..Inhibition of Erk, p70S6 kinase, and UBF1 by transfecting GECs with their respective inactive mutants abolished laminin ?1 synthesis, protein synthesis, and ..
  9. Hisatake K, Nishimura T, Maeda Y, Hanada K, Song C, Muramatsu M. Cloning and structural analysis of cDNA and the gene for mouse transcription factor UBF. Nucleic Acids Res. 1991;19:4631-7 pubmed
    The gene and protein structure of the mouse UBF (mUBF), a transcription factor for mouse ribosomal RNA gene, have been determined by cDNA and genomic clones. The unique mUBF gene consists of 21 exons spanning over 13 kb...
  10. Seither P, Iben S, Thiry M, Grummt I. PAF67, a novel protein that is associated with the initiation-competent form of RNA polymerase I. Biol Chem. 2001;382:1163-70 pubmed
    ..The results suggest that association of PAF67 with the 'core' enzyme endows Pol I with the capability to assemble into a productive transcription initiation complex at the rDNA promoter. ..
  11. Nonnekens J, Pérez Fernández J, Theil A, Gadal O, Bonnart C, Giglia Mari G. Mutations in TFIIH causing trichothiodystrophy are responsible for defects in ribosomal RNA production and processing. Hum Mol Genet. 2013;22:2881-93 pubmed publisher
    ..Our findings provide evidence that defective ribosome synthesis represents a new faulty mechanism involved in the pathophysiology of TFIIH-related diseases. ..
  12. Tu X, Baffa R, Luke S, Prisco M, Baserga R. Intracellular redistribution of nuclear and nucleolar proteins during differentiation of 32D murine hemopoietic cells. Exp Cell Res. 2003;288:119-30 pubmed
    ..studied were the insulin receptor substrate-1 (IRS-1), the ID2 protein, nucleolin, and the upstream binding factor (UBF), all of which are involved directly or indirectly in the differentiation program...
  13. Pistoni M, Verrecchia A, Doni M, Guccione E, Amati B. Chromatin association and regulation of rDNA transcription by the Ras-family protein RasL11a. EMBO J. 2010;29:1215-24 pubmed publisher
    ..the nucleolus of interphase mouse fibroblasts, where it co-localized with the RNA polymerase I-specific transcription factor UBF. Similar to UBF, RasL11a also marked the active subset of rDNA repeats (also called nucleolar organizers, ..
  14. Sun H, Tu X, Liu M, Baserga R. Dual regulation of upstream binding factor 1 levels by IRS-1 and ERKs in IGF-1-receptor signaling. J Cell Physiol. 2007;212:780-6 pubmed
    The Upstream Binding Factor 1 (UBF1) is a nucleolar protein that participates in the regulation of RNA polymerase I activity and ribosomal RNA (rRNA) synthesis...
  15. Young D, Hassan M, Pratap J, Galindo M, Zaidi S, Lee S, et al. Mitotic occupancy and lineage-specific transcriptional control of rRNA genes by Runx2. Nature. 2007;445:442-6 pubmed
    ..Runx2 chromosomal foci are associated with open chromatin, co-localize with the RNA Pol I transcription factor UBF1, and undergo transition into nucleoli at sites of rRNA synthesis during interphase...
  16. Drakas R, Tu X, Baserga R. Control of cell size through phosphorylation of upstream binding factor 1 by nuclear phosphatidylinositol 3-kinase. Proc Natl Acad Sci U S A. 2004;101:9272-6 pubmed
    ..Activation of UBF1 requires its phosphorylation...
  17. Valdez B, Henning D, So R, Dixon J, Dixon M. The Treacher Collins syndrome (TCOF1) gene product is involved in ribosomal DNA gene transcription by interacting with upstream binding factor. Proc Natl Acad Sci U S A. 2004;101:10709-14 pubmed
    ..we show that treacle is involved in ribosomal DNA gene transcription by interacting with upstream binding factor (UBF)...
  18. Wu A, Tu X, Prisco M, Baserga R. Regulation of upstream binding factor 1 activity by insulin-like growth factor I receptor signaling. J Biol Chem. 2005;280:2863-72 pubmed
    The upstream binding factor 1 (UBF1) is one of the proteins in a complex that regulates the activity of RNA polymerase I, which controls the rate of ribosomal RNA (rRNA) synthesis...
  19. van de Nobelen S, Rosa Garrido M, Leers J, Heath H, Soochit W, Joosen L, et al. CTCF regulates the local epigenetic state of ribosomal DNA repeats. Epigenetics Chromatin. 2010;3:19 pubmed publisher
    ..Using biotinylated CTCF as bait, we identified upstream binding factor (UBF) and multiple other components of the RNA polymerase I complex as potential CTCF-interacting partners...
  20. Brandenburger Y, Arthur J, Woodcock E, Du X, Gao X, Autelitano D, et al. Cardiac hypertrophy in vivo is associated with increased expression of the ribosomal gene transcription factor UBF. FEBS Lett. 2003;548:79-84 pubmed
    The ribosomal DNA transcription-specific factor, UBF, is a key target for the regulation of ribosomal RNA synthesis and hypertrophic growth of isolated neonatal cardiomyocytes...
  21. Wang Q, Miyakoda M, Yang W, Khillan J, Stachura D, Weiss M, et al. Stress-induced apoptosis associated with null mutation of ADAR1 RNA editing deaminase gene. J Biol Chem. 2004;279:4952-61 pubmed
  22. Achiron A, Mashiach R, Zilkha Falb R, Meijler M, Gurevich M. Polymerase I pathway inhibitor ameliorates experimental autoimmune encephalomyelitis. J Neuroimmunol. 2013;263:91-7 pubmed publisher
    ..Our findings demonstrate that POL1 pathway inhibition delayed and suppressed the development of EAE and ameliorated the disease in mice with persistent clinical signs. ..
  23. Koné M, Fleurot R, Chebrout M, Debey P, Beaujean N, Bonnet Garnier A. Three-Dimensional Distribution of UBF and Nopp140 in Relationship to Ribosomal DNA Transcription During Mouse Preimplantation Development. Biol Reprod. 2016;94:95 pubmed publisher
    ..study, we analyzed nucleologenesis during mouse early embryonic development using 3D-immunofluorescent detection of UBF and Nopp140, two proteins associated with different nucleolar compartments...
  24. Hamdane N, Tremblay M, Dillinger S, Stefanovsky V, Nemeth A, Moss T. Disruption of the UBF gene induces aberrant somatic nucleolar bodies and disrupts embryo nucleolar precursor bodies. Gene. 2017;612:5-11 pubmed publisher
    ..We found that disruption of the mouse Upstream Binding Factor (UBF (UBTF)) gene caused disassembly of somatic nucleoli and the accumulation of the key rRNA gene transcription factors into ..