Gene Symbol: Tnni1
Description: troponin I, skeletal, slow 1
Alias: 2700018B22Rik, AI747285, ssTnI, troponin I, slow skeletal muscle, troponin I, slow-twitch isoform
Species: mouse
Products:     Tnni1

Top Publications

  1. van Rooij E, Quiat D, Johnson B, Sutherland L, Qi X, Richardson J, et al. A family of microRNAs encoded by myosin genes governs myosin expression and muscle performance. Dev Cell. 2009;17:662-73 pubmed publisher
  2. Niro C, Demignon J, Vincent S, Liu Y, Giordani J, Sgarioto N, et al. Six1 and Six4 gene expression is necessary to activate the fast-type muscle gene program in the mouse primary myotome. Dev Biol. 2010;338:168-82 pubmed publisher
    ..This in vivo wide transcriptomal analysis of the function of the master myogenic determinants, Six, identifies them as novel markers for the differential activation of a specific muscle program during mammalian somitic myogenesis. ..
  3. Franco D, Icardo J. Molecular characterization of the ventricular conduction system in the developing mouse heart: topographical correlation in normal and congenitally malformed hearts. Cardiovasc Res. 2001;49:417-29 pubmed
    ..Our results do not provide evidence to support an extra-cardiac origin of the ventricular CS. ..
  4. Guenet J, Simon Chazottes D, Gravel M, Hastings K, Schiaffino S. Cardiac and skeletal muscle troponin I isoforms are encoded by a dispersed gene family on mouse chromosomes 1 and 7. Mamm Genome. 1996;7:13-5 pubmed
    ..The slow skeletal muscle troponin I locus (Tnni1) mapped to Chromosome (Chr) 1...
  5. MacLean D, Meedel T, Hastings K. Tissue-specific alternative splicing of ascidian troponin I isoforms. Redesign of a protein isoform-generating mechanism during chordate evolution. J Biol Chem. 1997;272:32115-20 pubmed
  6. Sakakibara I, Santolini M, Ferry A, Hakim V, Maire P. Six homeoproteins and a Iinc-RNA at the fast MYH locus lock fast myofiber terminal phenotype. PLoS Genet. 2014;10:e1004386 pubmed publisher
    ..Functional fast-sarcomeric unit formation is achieved by the coordinate expression of fast MYHs and linc-MYH, under the control of a common Six-bound enhancer. ..
  7. Delgado Olguin P, Huang Y, Li X, Christodoulou D, Seidman C, Seidman J, et al. Epigenetic repression of cardiac progenitor gene expression by Ezh2 is required for postnatal cardiac homeostasis. Nat Genet. 2012;44:343-7 pubmed publisher
    ..Our results suggest that epigenetic dysregulation in embryonic progenitor cells is a predisposing factor for adult disease and dysregulated stress responses. ..
  8. Hagiwara N, Ma B, Ly A. Slow and fast fiber isoform gene expression is systematically altered in skeletal muscle of the Sox6 mutant, p100H. Dev Dyn. 2005;234:301-11 pubmed
    ..Together with our earlier report, demonstrating early postnatal muscle defects in the Sox6 null-p100H mutant, the present results suggest that Sox6 likely plays an important role in muscle development...
  9. Liu Z, Li W, Ma X, Ding N, Spallotta F, Southon E, et al. Essential role of the zinc finger transcription factor Casz1 for mammalian cardiac morphogenesis and development. J Biol Chem. 2014;289:29801-16 pubmed publisher
    ..This suggests that CASZ1 is a novel 1p36 CHD gene and that the abnormal expression of cardiac morphogenesis and contraction genes induced by loss of Casz1 contributes to the heart defect. ..

Scientific Experts

More Information


  1. Daou N, Lecolle S, Lefebvre S, Della Gaspera B, Charbonnier F, Chanoine C, et al. A new role for the calcineurin/NFAT pathway in neonatal myosin heavy chain expression via the NFATc2/MyoD complex during mouse myogenesis. Development. 2013;140:4914-25 pubmed publisher
    ..Altogether, our findings demonstrate that the calcineurin/NFAT pathway plays a new role in establishing the early muscle fiber type in immature myofibers during embryogenesis. ..
  2. Potthoff M, Arnold M, McAnally J, Richardson J, Bassel Duby R, Olson E. Regulation of skeletal muscle sarcomere integrity and postnatal muscle function by Mef2c. Mol Cell Biol. 2007;27:8143-51 pubmed
    ..These results reveal a key role for Mef2c in maintenance of sarcomere integrity and postnatal maturation of skeletal muscle. ..
  3. Engel P, Kobayashi T, Biesiadecki B, Davis J, Tikunova S, Wu S, et al. Identification of a region of troponin I important in signaling cross-bridge-dependent activation of cardiac myofilaments. J Biol Chem. 2007;282:183-93 pubmed
    ..activation of force, especially at acidic pH, in myofilaments in which slow skeletal troponin I (ssTnI) replaced cardiac TnI (cTnI) indicates the significance of a His in ssTnI that is an homologous Ala in cTnI...
  4. Layland J, Cave A, Warren C, Grieve D, Sparks E, Kentish J, et al. Protection against endotoxemia-induced contractile dysfunction in mice with cardiac-specific expression of slow skeletal troponin I. FASEB J. 2005;19:1137-9 pubmed
    ..LPS) treatment in transgenic mice (TG) with cardiac-specific replacement of cTnI by slow skeletal TnI (ssTnI, which lacks the PKA phosphorylation sites) and matched nontransgenic littermates (NTG) on a CD1 background...
  5. Zhao W, Dhoot G. Both smooth and skeletal muscle precursors are present in foetal mouse oesophagus and they follow different differentiation pathways. Dev Dyn. 2000;218:587-602 pubmed
  6. Zhang W, Behringer R, Olson E. Inactivation of the myogenic bHLH gene MRF4 results in up-regulation of myogenin and rib anomalies. Genes Dev. 1995;9:1388-99 pubmed
    ..These results demonstrate an unanticipated regulatory relationship between myogenin and MRF4 and suggest that MRF4 influences rib outgrowth through an indirect mechanism. ..
  7. Pott C, Willkomm L, Grafweg S, Bölck B, Dorn G, Schwinger R, et al. Reduced troponin I phosphorylation and increased Ca(2+)-dependent ATP-consumption in triton X-skinned fiber preparations from Galphaq overexpressor mice. Mol Cell Biochem. 2008;314:133-41 pubmed publisher
    ..This may result from a decreased phosphorylation of troponin I in Galphaq-OE. ..
  8. Du J, Nan C, Huang J, Zhang C, Liu J, Jia P, et al. Functional characterization of mouse fetal TnI gene promoters in myocardial cells. J Biomed Sci. 2008;15:605-13 pubmed publisher
    Two major troponin I (TnI) genes, fetal TnI (ssTnI) and adult TnI (cTnI), are expressed in the mammalian heart under the control of a developmentally regulated program...
  9. Nassar R, Malouf N, Mao L, Rockman H, Oakeley A, Frye J, et al. cTnT1, a cardiac troponin T isoform, decreases myofilament tension and affects the left ventricular pressure waveform. Am J Physiol Heart Circ Physiol. 2005;288:H1147-56 pubmed
  10. Juretic N, Urzua U, Munroe D, Jaimovich E, Riveros N. Differential gene expression in skeletal muscle cells after membrane depolarization. J Cell Physiol. 2007;210:819-30 pubmed the expression of molecules involved in muscle cell structure were observed; K+-depolarization increased Tnni1 and Acta1 mRNA levels in both differentiated C2C12 and rat skeletal muscle cells in primary culture...
  11. Górnikiewicz B, Ronowicz A, Krzemiński M, Sachadyn P. Changes in gene methylation patterns in neonatal murine hearts: Implications for the regenerative potential. BMC Genomics. 2016;17:231 pubmed publisher
    ..This conclusion is supported by the evidence that an increase in DNA methylation in the neonatal murine heart from day 1 to day 7 occurs in the promoter regions of genes playing important roles in cardiovascular system development. ..
  12. Issa L, Palmer S, Guven K, Santucci N, Hodgson V, Popovic K, et al. MusTRD can regulate postnatal fiber-specific expression. Dev Biol. 2006;293:104-15 pubmed
    ..These data are consistent with our initial predictions for hMusTRD1alpha1 and suggest that slow fiber genes contain a specific common regulatory element that can be targeted by MusTRD proteins. ..
  13. Spies N, Smith C, Rodriguez J, Baker J, Batzoglou S, Sidow A. Constraint and divergence of global gene expression in the mammalian embryo. elife. 2015;4:e05538 pubmed publisher
    ..The widespread effect of maternal and embryonic genotype in conjunction with the purifying selection we uncovered suggests that embryogenesis is an important and understudied reservoir of phenotypic variation. ..
  14. Tsika R, Schramm C, Simmer G, Fitzsimons D, Moss R, Ji J. Overexpression of TEAD-1 in transgenic mouse striated muscles produces a slower skeletal muscle contractile phenotype. J Biol Chem. 2008;283:36154-67 pubmed publisher
    ..These novel in vivo data support a role for TEAD-1 in modulating slow muscle gene expression. ..
  15. Oh M, Rybkin I, Copeland V, Czubryt M, Shelton J, van Rooij E, et al. Calcineurin is necessary for the maintenance but not embryonic development of slow muscle fibers. Mol Cell Biol. 2005;25:6629-38 pubmed
    ..These results demonstrate that developmental patterning of slow fibers is independent of calcineurin, while the maintenance of the slow-fiber phenotype in the adult requires calcineurin activity...
  16. Braun T, Arnold H. Inactivation of Myf-6 and Myf-5 genes in mice leads to alterations in skeletal muscle development. EMBO J. 1995;14:1176-86 pubmed
    ..Our results provide evidence that skeletal myogenesis can proceed in the absence of two myogenic factors, Myf-5 and Myf-6, therefore they must exert largely non-redundant functions in vivo. ..
  17. Barton P, Mullen A, Cullen M, Dhoot G, Simon Chazottes D, Guenet J. Genes encoding troponin I and troponin T are organized as three paralogous pairs in the mouse genome. Mamm Genome. 2000;11:926-9 pubmed
  18. Chen Z, Friedrich G, Soriano P. Transcriptional enhancer factor 1 disruption by a retroviral gene trap leads to heart defects and embryonic lethality in mice. Genes Dev. 1994;8:2293-301 pubmed
    ..Although transcription of a number of muscle-specific genes believed to be TEF-1 targets appears normal, the defect in cardiogenesis is likely attributable to diminished transcription of one or several cardiac-specific genes. ..
  19. Zhu L, Lyons G, Juhasz O, Joya J, Hardeman E, Wade R. Developmental regulation of troponin I isoform genes in striated muscles of transgenic mice. Dev Biol. 1995;169:487-503 pubmed
    ..In addition, we provide the first evidence that the fast-twitch skeletal muscle isoform of troponin I, TnIf, is transiently expressed during early cardiac muscle development. ..
  20. Xu Y, Liu L, Pan B, Zhu J, Nan C, Huang X, et al. DNA methylation regulates mouse cardiac myofibril gene expression during heart development. J Biomed Sci. 2015;22:88 pubmed publisher
    ..studies have demonstrated that the expression of fetal troponin I gene (also called slow skeletal troponin I, ssTnI) is predominated in the fetal stage, reduced after birth and disappeared in the adulthood...
  21. Koppe R, Hallauer P, Karpati G, Hastings K. cDNA clone and expression analysis of rodent fast and slow skeletal muscle troponin I mRNAs. J Biol Chem. 1989;264:14327-33 pubmed
  22. Riedel B, Jia Y, Du J, Akerman S, Huang X. Thyroid hormone inhibits slow skeletal TnI expression in cardiac TnI-null myocardial cells. Tissue Cell. 2005;37:47-51 pubmed
    ..Two isoforms of TnI (the fetal form ssTnI and the adult form cTnI) are mainly expressed in the heart under a developmentally regulated program...
  23. Bröhl D, Vasyutina E, Czajkowski M, Griger J, Rassek C, Rahn H, et al. Colonization of the satellite cell niche by skeletal muscle progenitor cells depends on Notch signals. Dev Cell. 2012;23:469-81 pubmed publisher
    ..We conclude that Notch signals control homing of satellite cells, stimulating them to contribute to their own microenvironment and to adhere to myofibers. ..
  24. Huang X, Pi Y, Lee K, Henkel A, Gregg R, Powers P, et al. Cardiac troponin I gene knockout: a mouse model of myocardial troponin I deficiency. Circ Res. 1999;84:1-8 pubmed