Tlr5

Summary

Gene Symbol: Tlr5
Description: toll-like receptor 5
Species: mouse

Top Publications

  1. doi Metabolic syndrome and altered gut microbiota in mice lacking Toll-like receptor 5
    Matam Vijay-Kumar
    Department of Pathology, Emory University, Atlanta, GA 30322, USA
    Science 328:228-31. 2010
  2. pmc TLR5 signaling stimulates the innate production of IL-17 and IL-22 by CD3(neg)CD127+ immune cells in spleen and mucosa
    Laurye Van Maele
    Institut Pasteur de Lille, Centre d Infection et d Immunité de Lille, France
    J Immunol 185:1177-85. 2010
  3. pmc Control of Pseudomonas aeruginosa in the lung requires the recognition of either lipopolysaccharide or flagellin
    Reuben Ramphal
    Unité de Défense Innée et Inflammation, Institut Pasteur, Paris, France
    J Immunol 181:586-92. 2008
  4. pmc Toll-like receptor 5-deficient mice have dysregulated intestinal gene expression and nonspecific resistance to Salmonella-induced typhoid-like disease
    Matam Vijay-Kumar
    Department of Pathology, Emory University, Atlanta, GA 30322, USA
    Infect Immun 76:1276-81. 2008
  5. pmc Direct stimulation of tlr5+/+ CD11c+ cells is necessary for the adjuvant activity of flagellin
    John T Bates
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Immunol 182:7539-47. 2009
  6. pmc Deletion of TLR5 results in spontaneous colitis in mice
    Matam Vijay-Kumar
    Department of Pathology, Emory University, Atlanta, Georgia 30322, USA
    J Clin Invest 117:3909-21. 2007
  7. ncbi Cloning and characterization of the murine toll-like receptor 5 (Tlr5) gene: sequence and mRNA expression studies in Salmonella-susceptible MOLF/Ei mice
    G Sebastiani
    Department of Biochemistry, Center for the Study of Host Resistance, McGill University, Montreal General Hospital, Quebec, H3G 1A4, Canada
    Genomics 64:230-40. 2000
  8. pmc Involvement of Toll-like receptor 5 in the recognition of flagellated bacteria
    Vincent Feuillet
    Centre d immunologie de Marseille Luminy, Universite de la Mediterranee, Case 906, 13288 Marseille, France
    Proc Natl Acad Sci U S A 103:12487-92. 2006
  9. doi TLR5 is not required for flagellin-mediated exacerbation of DSS colitis
    Sabine M Ivison
    Division of Infectious Diseases, Department of Medicine, University of British Columbia, Vancouver, BC, Canada
    Inflamm Bowel Dis 16:401-9. 2010
  10. pmc Induction of adaptive immunity by flagellin does not require robust activation of innate immunity
    Catherine J Sanders
    Department of Pathology, Emory University, Atlanta, GA 30322, USA
    Eur J Immunol 39:359-71. 2009

Research Grants

Scientific Experts

  • Carles Ubeda
  • Irene Jarchum
  • T Eoin West
  • Cornelis van 't Veer
  • Christian Weile
  • Danielle Malo
  • Anna Riccioli
  • Scott C Wesselkamper
  • STEVEN MIZEL
  • THOMAS HAWN
  • Edward A Miao
  • Satoshi Uematsu
  • Benedicte Fournier
  • Paul L Bollyky
  • Shizuo Akira
  • Matam Vijay-Kumar
  • Andrew T Gewirtz
  • Luigi Franchi
  • Jesse D Aitken
  • Gabriel Nunez
  • Yoon Jeong Choi
  • Catherine J Sanders
  • Reuben Ramphal
  • Erica Andersen-Nissen
  • Cristiano Rumio
  • Richard A Flavell
  • Laure Janot
  • Jean Claude Sirard
  • Marco Palazzo
  • Megan E Himmel
  • Andrew S Neish
  • Thirumala Devi Kanneganti
  • Amal Amer
  • Lyudmila G Burdelya
  • Adriana Flores-Langarica
  • Melissa A Kinnebrew
  • Jorge Henao-Mejia
  • Kosuke Kato
  • Zhongcheng Shi
  • Johan Garaude
  • Jung Hee Joo
  • Magali Noval Rivas
  • Donna Kirkland
  • Ingrid Leiner
  • Eric G Pamer
  • Tobias M Hohl
  • Chao Shi
  • Mattias Enoksson
  • F A Carvalho
  • Shirdi E Letran
  • Felix Yarovinsky
  • N Oshima
  • Marcel R de Zoete
  • Yan Sun
  • Sabine M Ivison
  • Sang Hoon Rhee
  • Fu Shin Yu
  • Eunok Im
  • Mizuho Hasegawa
  • Christelle Langlet
  • Frederic A Carvalho
  • Laurye Van Maele
  • Young Sang Koh
  • Charalabos Pothoulakis
  • Bernhard Ryffel
  • Francois Erard
  • Andrea Balsari
  • Himanshu Kumar
  • Delphine Cayet
  • Amy E Morris
  • John T Bates
  • Gijs Hardenberg
  • Anna Rydström
  • Juan R Cubillos-Ruiz
  • Juan Luis Herrera
  • Megan K Levings
  • Eloïse Raoust
  • Shanshan Cai
  • Theodore S Steiner
  • Ildelfonso Vicente-Suarez
  • Shanthi V Sitaraman
  • A Marijke Keestra
  • Michel Chignard
  • Jos P M van Putten
  • Viviane Balloy
  • Naohiro Inohara
  • Clément Nempont
  • Ok S Shin
  • Hui Wu
  • Taro Kawai

Detail Information

Publications82

  1. doi Metabolic syndrome and altered gut microbiota in mice lacking Toll-like receptor 5
    Matam Vijay-Kumar
    Department of Pathology, Emory University, Atlanta, GA 30322, USA
    Science 328:228-31. 2010
    ..Here, we show that mice genetically deficient in Toll-like receptor 5 (TLR5), a component of the innate immune system that is expressed in the gut mucosa and that helps defend against ..
  2. pmc TLR5 signaling stimulates the innate production of IL-17 and IL-22 by CD3(neg)CD127+ immune cells in spleen and mucosa
    Laurye Van Maele
    Institut Pasteur de Lille, Centre d Infection et d Immunité de Lille, France
    J Immunol 185:1177-85. 2010
    ..In this study, we observed that the TLR5 agonist flagellin induced swift and transient transcription of genes encoding IL-17 and IL-22 in lymphoid, gut, and ..
  3. pmc Control of Pseudomonas aeruginosa in the lung requires the recognition of either lipopolysaccharide or flagellin
    Reuben Ramphal
    Unité de Défense Innée et Inflammation, Institut Pasteur, Paris, France
    J Immunol 181:586-92. 2008
    ..We conclude that the interactions of either TLR4 with LPS or TLR5 with flagellin can effectively defend the lung from P...
  4. pmc Toll-like receptor 5-deficient mice have dysregulated intestinal gene expression and nonspecific resistance to Salmonella-induced typhoid-like disease
    Matam Vijay-Kumar
    Department of Pathology, Emory University, Atlanta, GA 30322, USA
    Infect Immun 76:1276-81. 2008
    The recognition of flagellin by Toll-like receptor 5 (TLR5) is the dominant means by which model intestinal epithelia activate proinflammatory gene expression in response to Salmonella enterica...
  5. pmc Direct stimulation of tlr5+/+ CD11c+ cells is necessary for the adjuvant activity of flagellin
    John T Bates
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Immunol 182:7539-47. 2009
    ..of CD4(+) T cell adoptive transfers and bone marrow chimera mice in which the presence or absence of potential tlr5(+/+) CD11c(+) cells was controlled by injection of diphtheria toxin...
  6. pmc Deletion of TLR5 results in spontaneous colitis in mice
    Matam Vijay-Kumar
    Department of Pathology, Emory University, Atlanta, Georgia 30322, USA
    J Clin Invest 117:3909-21. 2007
    ..Here, we show that the flagellin receptor TLR5 has an essential and nonredundant role in protecting the gut from enteric microbes...
  7. ncbi Cloning and characterization of the murine toll-like receptor 5 (Tlr5) gene: sequence and mRNA expression studies in Salmonella-susceptible MOLF/Ei mice
    G Sebastiani
    Department of Biochemistry, Center for the Study of Host Resistance, McGill University, Montreal General Hospital, Quebec, H3G 1A4, Canada
    Genomics 64:230-40. 2000
    ..In this paper, we describe the cloning and characterization of the mouse homologue of human TLR5. Mouse Tlr5 encodes a 859-amino-acid protein that contains an N-terminal signal sequence, a leucine-rich repeat ..
  8. pmc Involvement of Toll-like receptor 5 in the recognition of flagellated bacteria
    Vincent Feuillet
    Centre d immunologie de Marseille Luminy, Universite de la Mediterranee, Case 906, 13288 Marseille, France
    Proc Natl Acad Sci U S A 103:12487-92. 2006
    ..b>TLR5 is a sensor for monomeric flagellin, which is a component of bacterial flagella known to be a virulence factor...
  9. doi TLR5 is not required for flagellin-mediated exacerbation of DSS colitis
    Sabine M Ivison
    Division of Infectious Diseases, Department of Medicine, University of British Columbia, Vancouver, BC, Canada
    Inflamm Bowel Dis 16:401-9. 2010
    ..Flagellin is also the only known ligand for Toll-like receptor 5 (TLR5), a key protein in innate immunity...
  10. pmc Induction of adaptive immunity by flagellin does not require robust activation of innate immunity
    Catherine J Sanders
    Department of Pathology, Emory University, Atlanta, GA 30322, USA
    Eur J Immunol 39:359-71. 2009
    ..Here, we examined the role of TLR5 and MyD88 in promoting innate and humoral immunity to flagellin using a prime/boost immunization regimen...
  11. ncbi Flagellin treatment protects against chemicals, bacteria, viruses, and radiation
    Matam Vijay-Kumar
    Department of Pathology, Emory University, Atlanta, GA 30322, USA
    J Immunol 180:8280-5. 2008
    ..We reasoned that, because it preferentially activates epithelial cells, the TLR5 agonist flagellin might not induce severe inflammatory pathology and yet be an ideal agent to provide such non-..
  12. ncbi Critical role for Ipaf in Pseudomonas aeruginosa-induced caspase-1 activation
    Luigi Franchi
    Department of Pathology and Comprehensive Cancer Center, The University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Eur J Immunol 37:3030-9. 2007
    ..flagellin was essential for caspase-1 and IL-1beta and this activity depended on Ipaf and the adaptor ASC but not TLR5. Notably, P. aeruginosa induced macrophage cell death and this event relied on flagellin and Ipaf but not on ASC...
  13. pmc Toll-like receptor 5-dependent regulation of inflammation in systemic Salmonella enterica Serovar typhimurium infection
    Benedicte Fournier
    Department of Pathology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Infect Immun 77:4121-9. 2009
    ..differences between WT and aflagellate Salmonella were highly attenuated in Toll-like receptor 5-deficient (TLR5(-/-)) mice, indicating involvement of TLR5-dependent signaling...
  14. ncbi Detection of pathogenic intestinal bacteria by Toll-like receptor 5 on intestinal CD11c+ lamina propria cells
    Satoshi Uematsu
    Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Suita Osaka 565 0871, Japan
    Nat Immunol 7:868-74. 2006
    Toll-like receptors (TLRs) recognize distinct microbial components and induce innate immune responses. TLR5 is triggered by bacterial flagellin. Here we generated Tlr5-/- mice and assessed TLR5 function in vivo...
  15. ncbi Cytoplasmic flagellin activates caspase-1 and secretion of interleukin 1beta via Ipaf
    Edward A Miao
    Institute for Systems Biology, Seattle, Washington 98103, USA
    Nat Immunol 7:569-75. 2006
    ....
  16. ncbi Cytosolic flagellin requires Ipaf for activation of caspase-1 and interleukin 1beta in salmonella-infected macrophages
    Luigi Franchi
    Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Nat Immunol 7:576-82. 2006
    ..Thus, detection of flagellin through Ipaf induces caspase-1 activation independently of Toll-like receptor 5 in salmonella-infected and lipopolysaccharide-tolerized macrophages...
  17. ncbi Altered inflammatory responses in TLR5-deficient mice infected with Legionella pneumophila
    Thomas R Hawn
    Department of Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA
    J Immunol 179:6981-7. 2007
    ..We examined the role of TLR5 during the murine response to aerosolized Lp infection...
  18. doi Simultaneous targeting of toll- and nod-like receptors induces effective tumor-specific immune responses
    Johan Garaude
    Immunology Institute, Department of Medicine, The Tisch Cancer Institute, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA
    Sci Transl Med 4:120ra16. 2012
    ..tumor cells constitutes an effective antitumor vaccination strategy that relies on simultaneous engagement of TLR5 and the Nod-like receptors (NLRs) NLRC4/NAIP5 (neuronal apoptosis inhibitory protein 5) by flagellin along with ..
  19. doi Induction of Paneth cell degranulation by orally administered Toll-like receptor ligands
    Cristiano Rumio
    Department of Human Morphology and Biomedical Sciences Città Studi University of Milan, Milan, Italy
    J Cell Physiol 227:1107-13. 2012
    ..The TLR3 agonist polyinosinic-polycytidylic acid also induced rapid degranulation, whereas the TLR4 and TLR5 agonists LPS and flagellin, respectively, induced late degranulation mediated by TNF-α...
  20. pmc Central role of liver in anticancer and radioprotective activities of Toll-like receptor 5 agonist
    Lyudmila G Burdelya
    Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Proc Natl Acad Sci U S A 110:E1857-66. 2013
    Vertebrate Toll-like receptor 5 (TLR5) recognizes bacterial flagellin proteins and activates innate immune responses to motile bacteria...
  21. doi Systemic flagellin immunization stimulates mucosal CD103+ dendritic cells and drives Foxp3+ regulatory T cell and IgA responses in the mesenteric lymph node
    Adriana Flores-Langarica
    Medical Research Council Centre for Immune Regulation, Division of Immunity and Infection, Institute of Biomedical Research, University of Birmingham, Birmingham B15 2TT, United Kingdom
    J Immunol 189:5745-54. 2012
    ..Salmonella enterica serovar Typhimurium (FliC) can impact markedly on host immunity, in part via its recognition by TLR5. In this study, we show that systemic immunization with soluble FliC (sFliC) drives distinct immune responses ..
  22. pmc Interleukin 23 production by intestinal CD103(+)CD11b(+) dendritic cells in response to bacterial flagellin enhances mucosal innate immune defense
    Melissa A Kinnebrew
    Infectious Diseases Service, Department of Medicine, Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Immunity 36:276-87. 2012
    ..Systemic administration of flagellin, a bacterial protein that stimulates Toll-like receptor 5 (TLR5), induces epithelial expression of RegIIIγ and protects mice from intestinal colonization with antibiotic-..
  23. pmc B cell-intrinsic MyD88 signaling prevents the lethal dissemination of commensal bacteria during colonic damage
    Donna Kirkland
    Department of Immunology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    Immunity 36:228-38. 2012
    ..Our results indicate that the lack of intact MyD88 signaling in B cells, coupled with impaired epithelial integrity, enables commensal bacteria to function as highly pathogenic organisms, causing rapid host death...
  24. pmc Membrane-tethered MUC1 mucin is phosphorylated by epidermal growth factor receptor in airway epithelial cells and associates with TLR5 to inhibit recruitment of MyD88
    Kosuke Kato
    Department of Physiology, Center for Inflammation, Translational and Clinical Lung Research, Temple University School of Medicine, Philadelphia, PA 19140, USA
    J Immunol 188:2014-22. 2012
    ..In this article, we elucidate the mechanism by which MUC1 inhibits TLR5 signaling...
  25. pmc MyD88 is critically involved in immune tolerance breakdown at environmental interfaces of Foxp3-deficient mice
    Magali Noval Rivas
    Division of Immunology, Allergy and Rheumatology, Department of Pediatrics, The David Geffen School of Medicine at the University of California at Los Angeles, Los Angeles, California, USA
    J Clin Invest 122:1933-47. 2012
    ..Moreover, microbial factors may play a substantial role in the pathogenesis of human autoimmune disease resulting from Treg deficiency...
  26. doi Dual oxidase 2 is essential for the toll-like receptor 5-mediated inflammatory response in airway mucosa
    Jung Hee Joo
    Research Center for Natural Human Defense System, Yonsei University College of Medicine, Seoul, South Korea
    Antioxid Redox Signal 16:57-70. 2012
    ..However, the molecular mechanism between TLR and NOX-mediated reactive oxygen species (ROS) production in human airway mucosa has been poorly understood...
  27. pmc Familial transmission rather than defective innate immunity shapes the distinct intestinal microbiota of TLR-deficient mice
    Carles Ubeda
    Infectious Diseases Service, Department of Medicine, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    J Exp Med 209:1445-56. 2012
    ..Comparison of MyD88-, TLR2-, TLR4-, TLR5-, and TLR9-deficient mice and their respective wild-type (WT) littermates demonstrated that the impact of TLR ..
  28. pmc Toll-like receptor 11 (TLR11) prevents Salmonella penetration into the murine Peyer patches
    Zhongcheng Shi
    Texas A and M University Health Science Center, Houston, Texas 77030, USA
    J Biol Chem 287:43417-23. 2012
    ..Here, we report that, in contrast to TLR5 as a "carrier of Salmonella," TLR11 works as a "blocker of Salmonella" to prevent highly invasive Salmonella from ..
  29. pmc Inflammasome-mediated dysbiosis regulates progression of NAFLD and obesity
    Jorge Henao-Mejia
    Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Nature 482:179-85. 2012
    ....
  30. pmc Flagellin stimulates protective lung mucosal immunity: role of cathelicidin-related antimicrobial peptide
    Fu Shin Yu
    Department of Ophthalmology, Wayne State University, Detroit, MI 48201, USA
    J Immunol 185:1142-9. 2010
    ..In this study, we evaluated the effect of the TLR5 ligand flagellin on stimulation of antibacterial mucosal immunity in a lethal murine Pseudomonas aeruginosa ..
  31. pmc Bone marrow mesenchymal stem and progenitor cells induce monocyte emigration in response to circulating toll-like receptor ligands
    Chao Shi
    Immunology Program, Sloan Kettering Institute, Infectious Diseases Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Immunity 34:590-601. 2011
    ..Our findings suggest that bone marrow MSCs and CAR cells respond to circulating microbial molecules and regulate bloodstream monocyte frequencies by secreting MCP1 in proximity to bone marrow vascular sinuses...
  32. pmc TRIF modulates TLR5-dependent responses by inducing proteolytic degradation of TLR5
    Yoon Jeong Choi
    Division of Digestive Diseases, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 285:21382-90. 2010
    ..an adaptor molecule mediating TLR3 signaling and MyD88-independent signaling of TLR4, plays an inhibitory role in TLR5-elicited responses by inducing proteolytic degradation of TLR5...
  33. doi Toll-like receptor stimulation differentially regulates vasoactive intestinal peptide type 2 receptor in macrophages
    Juan Luis Herrera
    CABIMER Andalusian Center for Molecular Biology and Regenerative Medicine, CSIC University of Seville UPO Junta de Andalucia, Seville, Spain
    J Cell Mol Med 13:3209-17. 2009
    ..Remarkably, imiquimod--a synthetic TLR7 ligand--led to a potent up-regulation of VPAC(2) gene expression. TLR5 stimulation by flagellin present in gram-positive and gram-negative bacteria did not affect VPAC(2) mRNA...
  34. pmc Reconstitution of a functional Toll-like receptor 5 binding site in Campylobacter jejuni flagellin
    Marcel R de Zoete
    Department of Infectious Diseases and Immunology, Utrecht University, TD Utrecht, The Netherlands
    J Biol Chem 285:12149-58. 2010
    ..Flagellins from most species activate Toll-like receptor 5 (TLR5)...
  35. doi PKC-alpha controls MYD88-dependent TLR/IL-1R signaling and cytokine production in mouse and human dendritic cells
    Christelle Langlet
    Institute for Medical Immunology, Universite Libre de Bruxelles, Charleroi, Belgium
    Eur J Immunol 40:505-15. 2010
    ..or HBDDe, downregulated the production of classical inflammatory/immunomodulatory cytokines induced by TLR2, TLR5 or IL-1R but not by TLR3 stimulation...
  36. pmc MyD88-dependent signaling contributes to host defense against ehrlichial infection
    Young Sang Koh
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts, United States of America
    PLoS ONE 5:e11758. 2010
    ....
  37. pmc TLR4 and TLR5 on corneal macrophages regulate Pseudomonas aeruginosa keratitis by signaling through MyD88-dependent and -independent pathways
    Yan Sun
    Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, OH 44106, USA
    J Immunol 185:4272-83. 2010
    ..PAO1, we found that F4/80(+) macrophages were the predominant cell type in the cornea expressing TLR2, TLR4, and TLR5. Depletion of macrophages and dendritic cells using transgenic Mafia mice, in which Fas ligand is selectively ..
  38. pmc Toll-like receptor 5 stimulation protects mice from acute Clostridium difficile colitis
    Irene Jarchum
    MImmunology Program, Department of Medicine Division of Infectious Disease, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Infect Immun 79:1498-503. 2011
    ..We found that administration of purified Salmonella-derived flagellin, a Toll-like receptor 5 (TLR5) agonist, protects mice from C. difficile colitis by delaying C...
  39. doi Mast cells as sensors of cell injury through IL-33 recognition
    Mattias Enoksson
    Clinical Immunology and Allergy Unit, Department of Medicine, Karolinska Institute, SE 171 76 Stockholm, Sweden
    J Immunol 186:2523-8. 2011
    ..Our findings identify IL-33 as a key danger signal released by necrotic structural cells capable of activating mast cells, thus providing novel insights concerning the role of mast cells as sensors of cell injury...
  40. pmc Delineation of the role of Toll-like receptor signaling during peritonitis by a gradually growing pathogenic Escherichia coli
    Cornelis van 't Veer
    Center for Infection and Immunity Amsterdam, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
    J Biol Chem 286:36603-18. 2011
    ..coli peritonitis models. The present study displays how the kinetics of pro- and anti-inflammatory mechanisms are regulated by TLRs during peritonitis by a gradually growing E. coli load and how these kinetics may affect outcome...
  41. pmc Glucose activation of islets of Langerhans up-regulates Toll-like receptor 5: possible mechanism of protection
    C Weile
    The Bartholin Institute, Rigshospitalet, Copenhagen, Denmark
    Clin Exp Immunol 166:251-7. 2011
    ..We have found that the expression of TLR5 is up-regulated by glucose activation of isolated islets of Langerhans, in contrast to other investigated TLRs (TLR-..
  42. pmc TLR5 functions as an endocytic receptor to enhance flagellin-specific adaptive immunity
    Shirdi E Letran
    Center for Infectious Diseases and Microbiology Translational Research, Department of Medicine, Division of Gastroenterology, Hepatology and Nutrition, McGuire Translational Research Facility, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Eur J Immunol 41:29-38. 2011
    ..Here, we demonstrate a previously unknown function for TLR5, namely that it enhances MHC class-II presentation of flagellin epitopes to CD4(+) T cells and is required for ..
  43. pmc TLR5 or NLRC4 is necessary and sufficient for promotion of humoral immunity by flagellin
    Matam Vijay-Kumar
    Department of Pathology, Emory University, Atlanta, GA 30322, USA
    Eur J Immunol 40:3528-34. 2010
    ..flagellin to induce cytokines and elicit/promote murine antibody responses upon deletion of the flagellin receptors TLR5 and/or NLRC4 (also referred to as IPAF) using a prime/boost regimen...
  44. pmc Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria
    Edward A Miao
    Institute for Systems Biology, Seattle, Washington, USA
    Nat Immunol 11:1136-42. 2010
    ..This demonstrates that activation of caspase-1 clears intracellular bacteria in vivo independently of IL-1β and IL-18 and establishes pyroptosis as an efficient mechanism of bacterial clearance by the innate immune system...
  45. pmc TRIF mediates Toll-like receptor 5-induced signaling in intestinal epithelial cells
    Yoon Jeong Choi
    Division of Digestive Diseases, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 285:37570-8. 2010
    ..However, TLR5, the specific receptor for flagellin, is known to utilize only MyD88 to elicit inflammatory responses, and an ..
  46. pmc TLR5 activation induces secretory interleukin-1 receptor antagonist (sIL-1Ra) and reduces inflammasome-associated tissue damage
    F A Carvalho
    Department of Pathology, Emory University School of Medicine, Atlanta, Georgia, USA
    Mucosal Immunol 4:102-11. 2011
    Toll-like receptor-5 (TLR5)-mediated detection of flagellin induces nuclear factor (NF)-κB-mediated transcription of host defense gene expression, whereas recognition of intracellular flagellin by interleukin (IL)-1-converting enzyme ..
  47. pmc Transitions in oral and intestinal microflora composition and innate immune receptor-dependent stimulation during mouse development
    Mizuho Hasegawa
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Infect Immun 78:639-50. 2010
    ..The major stimulatory activity of the intestinal microflora was still intact in NOD1-, NOD2-, TLR2-, TLR4-, TLR5-, TLR9-, TLR11-, ASC-, or RICK-deficient cells but still relied on the adaptor MyD88...
  48. ncbi Characterization of the mouse genes for mitochondrial transcription factors B1 and B2
    Anja Rantanen
    Department of Medical Nutrition, Karolinska Institutet, Novum, Huddinge Hospital, S 14186 Huddinge, Sweden
    Mamm Genome 14:1-6. 2003
    ....
  49. ncbi Ligand-induced differential cross-regulation of Toll-like receptors 2, 4 and 5 in intestinal epithelial cells
    Remon A M H van Aubel
    Department of Infectious Diseases and Immunology, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    Mol Immunol 44:3702-14. 2007
    Toll-like receptors (TLR) 2, TLR4 and TLR5 are primary mucosal sensors of microbial patterns...
  50. ncbi Cutting edge: Tlr5-/- mice are more susceptible to Escherichia coli urinary tract infection
    Erica Andersen-Nissen
    Institute for Systems Biology, Seattle, WA 98103, USA
    J Immunol 178:4717-20. 2007
    Although TLR5 regulates the innate immune response to bacterial flagellin, it is unclear whether its function is essential during in vivo murine infections...
  51. ncbi Activation of enteroendocrine cells via TLRs induces hormone, chemokine, and defensin secretion
    Marco Palazzo
    Department of Human Morphology, Institute of Pathology, Universita degli Studi di Milano, Via Mangiagalli 31, Milan, Italy
    J Immunol 178:4296-303. 2007
    ....
  52. ncbi Variable requirement of dendritic cells for recruitment of NK and T cells to different TLR agonists
    Takefumi Uchida
    Department of Surgery, University of Florida College of Medicine, 1600 SW Archer Road, Gainesville, FL 32610, USA
    J Immunol 178:3886-92. 2007
    ..subsets of DCs express a variety of TLRs, with conventional DCs responding in vitro to predominantly TLR3, TLR4, TLR5, and TLR9 ligands, and plasmacytoid DCs responding mainly to TLR7 and TLR9 ligands...
  53. pmc A conserved surface on Toll-like receptor 5 recognizes bacterial flagellin
    Erica Andersen-Nissen
    Institute for Systems Biology, Seattle, WA 98103, USA
    J Exp Med 204:393-403. 2007
    ..We demonstrate that mouse and human TLR5 discriminate between different flagellins, and we use this difference to map the flagellin recognition site on TLR5 ..
  54. ncbi MyD88-dependent and MyD88-independent pathways in synergy, priming, and tolerance between TLR agonists
    Aranya Bagchi
    Department of Anesthesia and Critical Care, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA
    J Immunol 178:1164-71. 2007
    ..Pam3Cys, flagellin, and CpG DNA, which act through TLR2 (peptidoglycan-associated lipoprotein and Pam3Cys), TLR5, and TLR9, respectively. D and I agonists were markedly synergistic in inducing cytokine production in vivo in mice...
  55. ncbi Pathological role of Toll-like receptor signaling in cerebral malaria
    Cevayir Coban
    Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Int Immunol 19:67-79. 2007
    ..These results strongly suggest that TLR2- and/or TLR9-mediated, MyD88-dependent brain pathogenesis may play a critical role in CM, the lethal complication during PbA infection...
  56. ncbi Sertoli cells initiate testicular innate immune responses through TLR activation
    Anna Riccioli
    Department of Histology and Medical Embryology, Istituto Pasteur Fondazione Cenci Bolognetti, University of Rome La Sapienza, Rome, Italy
    J Immunol 177:7122-30. 2006
    ..We measured the expression of TLR2, TLR4, TLR5, and TLR6 mRNAs and confirmed by FACS analysis the presence of proteins TLR2 and TLR5 on which we focused our study...
  57. ncbi Regulation of Legionella phagosome maturation and infection through flagellin and host Ipaf
    Amal Amer
    Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Biol Chem 281:35217-23. 2006
    ..Activation of caspase-1 in response to Legionella infection also required host Ipaf, but not TLR5. In the absence of Ipaf or caspase-1 activation, the LCP acquired endoplasmic reticulum-derived vesicles, avoided ..
  58. ncbi Tlr5 is not primarily associated with susceptibility to Salmonella Typhimurium infection in MOLF/Ei mice
    Isabelle Angers
    Department of Human Genetics, McGill University, Montreal, Quebec, Canada H3G 1A4
    Mamm Genome 17:385-97. 2006
    ..A member of the Toll-like receptors family, Tlr5, located on distal Chromosome 1, was previously shown to be a candidate gene for Ity3 based on expression studies ..
  59. ncbi Gangliosides inhibit flagellin signaling in the absence of an effect on flagellin binding to toll-like receptor 5
    A Phillip West
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    J Biol Chem 280:9482-8. 2005
    ..12213-12219) concluded that gangliosides serve as co-receptors for flagellin signaling via toll-like receptor 5 (TLR5)...
  60. ncbi Enhanced egg-induced immunopathology correlates with high IFN-gamma in murine schistosomiasis: identification of two epistatic genetic intervals
    Laura I Rutitzky
    Department of Pathology, Tufts University School of Medicine, 150 Harrison Avenue, Boston, MA 02111, USA
    J Immunol 174:435-40. 2005
    ....
  61. ncbi In vitro and ex vivo activation of the TLR5 signaling pathway in intestinal epithelial cells by a commensal Escherichia coli strain
    Jean Christophe Bambou
    INSERM, EMI 0212, 156 rue de Vaugirard, 75015 Paris, France
    J Biol Chem 279:42984-92. 2004
    ..MG1655 flagellin was necessary and sufficient to trigger this pro-inflammatory pathway via its interaction with TLR5 and the subsequent recruitment of the adaptor protein MyD88...
  62. ncbi Induction of macrophage nitric oxide production by Gram-negative flagellin involves signaling via heteromeric Toll-like receptor 5/Toll-like receptor 4 complexes
    Steven B Mizel
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Immunol 170:6217-23. 2003
    The induction of cytokine synthesis by flagellin is mediated by a Toll-like receptor 5 (TLR5) signaling pathway...
  63. ncbi Blocking of the TLR5 activation domain hampers protective potential of flagellin DNA vaccine
    Sukumar Saha
    Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, 3 9 Fukuura, Kanazawa Ku, Yokohama, Japan
    J Immunol 179:1147-54. 2007
    ..is readily produced and manipulated as a recombinant protein and has intrinsic adjuvant activity mediated through TLR5. Although DNA vaccines encoding native Pseudomonas B-type (FliC) or A-type (FlaA) flagellin are strongly ..
  64. pmc Distinct roles for MyD88 and Toll-like receptors 2, 5, and 9 in phagocytosis of Borrelia burgdorferi and cytokine induction
    Ok S Shin
    Department of Pathology Immunology, Sackler School for Graduate Biomedical Studies, Tufts University, 155 Harrison Avenue, Boston, Massachusetts 02111, USA
    Infect Immun 76:2341-51. 2008
    ..burgdorferi. Knockouts of TLR2 and TLR9 or knockdown of TLR5 by small interfering RNA produced no defects in phagocytosis of B. burgdorferi...
  65. doi Expression patterns and functions of toll-like receptors in mouse sertoli cells
    Hui Wu
    Department of Cell Biology, School of Basic Medicine, Peking Union Medical College, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, People s Republic of China
    Endocrinology 149:4402-12. 2008
    ..We demonstrated that TLR2, TLR3, TLR4, and TLR5 are highly expressed; TLR6, TLR7, and TLR13 are expressed at relatively low level; and TLR1, TLR8, TLR9, TLR11, and ..
  66. pmc Role of Toll-like receptor 5 in the innate immune response to acute P. aeruginosa pneumonia
    Amy E Morris
    Harborview Medical Center, Seattle, WA 98104, USA
    Am J Physiol Lung Cell Mol Physiol 297:L1112-9. 2009
    ..The contribution of Toll-like receptor 5 (TLR5) to the innate immune response to this organism is incompletely understood...
  67. pmc Toll-like receptor signaling in airborne Burkholderia thailandensis infection
    T Eoin West
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Washington School of Medicine, Seattle, Washington 98104, USA
    Infect Immun 77:5612-22. 2009
    ..thailandensis pneumonia. TLR2, TLR4, and TLR5 mediate NF-kappaB activation by B. thailandensis in transfected HEK293 or CHO cells...
  68. ncbi Deletion of flagellin's hypervariable region abrogates antibody-mediated neutralization and systemic activation of TLR5-dependent immunity
    Clément Nempont
    Institut National de la Sante et de la Recherche Medicale, Unité 801, Institut Pasteur de Lille, Universite de Lille 2, Lille, France
    J Immunol 181:2036-43. 2008
    ..is a unique MAMP because it harbors 1) an antigenic hypervariable region and 2) a conserved domain involved in TLR5-dependent systemic and mucosal proinflammatory and adjuvant activities...
  69. pmc TLR5 ligation by flagellin converts tolerogenic dendritic cells into activating antigen-presenting cells that preferentially induce T-helper 1 responses
    Ildelfonso Vicente-Suarez
    Division of Immunology and Division of Malignant Hematology, H Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, United States
    Immunol Lett 125:114-8. 2009
    ..Here we show for the first time that Tol-DCs express higher levels of TLR5 mRNA, but not TLR4 or TLR9 mRNA relative to DCs differentiated with GM-CSF and IL-4 (BM-DCs)...
  70. doi Pathogen recognition in the innate immune response
    Himanshu Kumar
    Laboratory of Host Defense, WPI Immunology Frontier Research Center, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Biochem J 420:1-16. 2009
    ..In the present review, we will discuss the recent progress in the study of pathogen recognition by TLRs, RLRs and NLRs and their signalling pathways...
  71. pmc The toll-like receptor signaling molecule Myd88 contributes to pancreatic beta-cell homeostasis in response to injury
    Paul L Bollyky
    Benaroya Research Institute, Seattle, Washington, United States of America
    PLoS ONE 4:e5063. 2009
    ..These data suggest that Myd88 signaling and certain TLR ligands mediate a homeostatic effect on beta-cells primarily in the setting of injury...
  72. pmc Myeloid differentiation protein-2-dependent and -independent neutrophil accumulation during Escherichia coli pneumonia
    Shanshan Cai
    Laboratory of Lung Biology, Department of Pathobiological Sciences and Center for Experimental Infectious Disease Research, Louisiana State University LSU, Baton Rouge, Louisiana 70803, USA
    Am J Respir Cell Mol Biol 40:701-9. 2009
    ..coli exhibited attenuated neutrophil accumulation, whereas MD-2/TLR5(-/-) mice inoculated with E. coli showed further attenuated neutrophil influx and impaired bacterial clearance...
  73. doi Regulation of humoral and cellular gut immunity by lamina propria dendritic cells expressing Toll-like receptor 5
    Satoshi Uematsu
    Laboratory of Host Defense, Immunology Frontier Research Center, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Nat Immunol 9:769-76. 2008
    ..identify a subset of CD11c(hi)CD11b(hi) lamina propria dendritic cells (LPDCs) that expressed Toll-like receptor 5 (TLR5) in the small intestine...
  74. pmc The role of T-regulatory cells and Toll-like receptors in the pathogenesis of human inflammatory bowel disease
    Megan E Himmel
    Department of Surgery, University of British Columbia, and Immunity and Infection Research Centre, Vancouver Coastal Health Research Institute, Vancouver, Canada
    Immunology 125:145-53. 2008
    ..In addition, we discuss evidence that direct signaling via TLRs to T cells can affect IBD and that T-cell-dependent responses to bacterial proteins, such as flagellin, are central to the aetiology of this disease...
  75. doi Radioresistant cells expressing TLR5 control the respiratory epithelium's innate immune responses to flagellin
    Laure Janot
    University of Orleans and CNRS, Molecular Immunology and Embryology, UMR 6218, Institut de Transgénose, Orleans, France
    Eur J Immunol 39:1587-96. 2009
    ..Flagellin-induced signalling involves TLR5 and MyD88 and, according to some reports, TLR4...
  76. doi Monocyte and neutrophil recruitment during oral Salmonella infection is driven by MyD88-derived chemokines
    Anna Rydström
    Department of Microbiology and Immunology, Goteborg University, Goteborg, Sweden
    Eur J Immunol 39:3019-30. 2009
    ..phagocyte recruitment to PP and MLN was intact in mice lacking TLR4 alone and when signaling through TLR4 and TLR5 was simultaneously absent; however, recruitment was compromised in MyD88(-/-) and more so in MyD88(-/-)TLR4(-/-) ..
  77. pmc Pseudomonas aeruginosa LPS or flagellin are sufficient to activate TLR-dependent signaling in murine alveolar macrophages and airway epithelial cells
    Eloïse Raoust
    Unité de Défense Innée et Inflammation, Institut Pasteur, Paris, France
    PLoS ONE 4:e7259. 2009
    ..Based on whole animal experiments, using TLR knockout mice, the control of this bacterium is believed to occur by the recognition of LPS and flagellin by TLRs 2,4 and 5, respectively...
  78. pmc Quantitative trait analysis of the development of pulmonary tolerance to inhaled zinc oxide in mice
    Scott C Wesselkamper
    Department of Environmental Health, University of Cincinnati Medical Center, Cincinnati, OH 45267, USA
    Respir Res 6:73. 2005
    ..The BALB/cByJ (CBy) and DBA/2J (D2) strains were identified as tolerant and non-tolerant, respectively. The present study was designed to identify candidate genes that control the development of pulmonary tolerance to inhaled ZnO...
  79. pmc Polyethylenimine-based siRNA nanocomplexes reprogram tumor-associated dendritic cells via TLR5 to elicit therapeutic antitumor immunity
    Juan R Cubillos-Ruiz
    Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, New Hampshire, USA
    J Clin Invest 119:2231-44. 2009
    ..PEI triggered robust and selective TLR5 activation in vitro and elicited the production of hallmark TLR5-inducible cytokines in WT mice, but not in Tlr5-/- ..
  80. doi Immune responses of TLR5(+) lamina propria dendritic cells in enterobacterial infection
    Satoshi Uematsu
    Laboratory of Host Defense, WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan
    J Gastroenterol 44:803-11. 2009
    Toll-like receptors (TLRs) recognize distinct microbial components and induce innate immune responses. TLR5 has been shown to recognize bacterial flagellin...
  81. pmc A20 is an early responding negative regulator of Toll-like receptor 5 signalling in intestinal epithelial cells during inflammation
    N Oshima
    Department of Internal Medicine II, Shimane University School of Medicine, Izumo, Shimane, Japan
    Clin Exp Immunol 159:185-98. 2010
    ..Our results provide new insights into the negative feedback regulation of TLR-5 signalling that maintains the innate immune system in the gut...

Research Grants16

  1. TLR5 and Pulmonary Innate Immunity
    THOMAS HAWN; Fiscal Year: 2009
    ..We have also shown that approximately 10% of individuals have a TLRS stop codon polymorphism (TLR5-392STOP) that inhibits flagellin signaling in a dominant fashion and is associated with susceptibility to pneumonia ..
  2. TRAINING PROGRAM IN IMMUNOLOGY AND PATHOGENESIS
    STEVEN MIZEL; Fiscal Year: 2007
    ..The success of the training program is evidenced by the very strong publication record of the trainees and the very high quality of their subsequent postdoctoral positions. ..
  3. Preclinical Development of Flagellin as a Mucosal Adjuvant in Biodefense Vaccines
    STEVEN MIZEL; Fiscal Year: 2007
    ..The completion of these aims will provide a very strong foundation for the clinical evaluation of the mucosal flagellin/Y. pestis vaccine in humans. ..
  4. TLR5 and Pulmonary Innate Immunity
    THOMAS HAWN; Fiscal Year: 2007
    ..We have also shown that approximately 10% of individuals have a TLRS stop codon polymorphism (TLR5-392STOP) that inhibits flagellin signaling in a dominant fashion and is associated with susceptibility to pneumonia ..
  5. Activation of inflammatory cells by flagellin
    STEVEN MIZEL; Fiscal Year: 2006
    ..Flagellin signaling is mediated by a toll-like receptor (TLR) protein, TLR5, but can be modulated by another TLR, TLR4...
  6. Toll-like Receptors and the Innate Immune Response
    THOMAS HAWN; Fiscal Year: 2003
    ..tuberculosis and L. major will reveal novel insights about innate immunity and infectious diseases that will have implications for vaccine development. ..