Genomes and Genes
Gene Symbol: Tjp1
Description: tight junction protein 1
Alias: ZO1, tight junction protein ZO-1, zona occludens protein 1, zonula occludens protein 1
- Zhadanov A, Provance D, Speer C, Coffin J, Goss D, Blixt J, et al. Absence of the tight junctional protein AF-6 disrupts epithelial cell-cell junctions and cell polarity during mouse development. Curr Biol. 1999;9:880-8 pubmed..The loss of neuroepithelial polarity in mutants is consistent with a loss of efficacy of the cell-cell junctions that have a critical role in establishing apical/basolateral asymmetry. ..
- Cain S, Martinez G, Kokkinos M, Turner K, Richardson R, Abud H, et al. Differential requirement for beta-catenin in epithelial and fiber cells during lens development. Dev Biol. 2008;321:420-33 pubmed publisher..cells displayed altered apical-basal polarity as evidenced by altered distribution of the tight junction protein, ZO1, disruption of apical actin filaments and abnormal deposition of extracellular matrix, resulting in a deficient ..
- Umeda K, Matsui T, Nakayama M, Furuse K, Sasaki H, Furuse M, et al. Establishment and characterization of cultured epithelial cells lacking expression of ZO-1. J Biol Chem. 2004;279:44785-94 pubmed..Finally, we discussed advantageous aspects of the gene knock-out system with cultured epithelial cells in epithelial cell biology. ..
- Furuse M, Itoh M, Hirase T, Nagafuchi A, Yonemura S, Tsukita S. Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions. J Cell Biol. 1994;127:1617-26 pubmed..The coincidence of the sequence necessary for the ZO-1 association with that for the TJ localization suggests that the association with underlying cytoskeletons through ZO-1 is required for occludin to be localized at TJ. ..
- Hieda Y, Iwai K, Morita T, Nakanishi Y. Mouse embryonic submandibular gland epithelium loses its tissue integrity during early branching morphogenesis. Dev Dyn. 1996;207:395-403 pubmed
- Kan N, Stemmler M, Junghans D, Kanzler B, de Vries W, Dominis M, et al. Gene replacement reveals a specific role for E-cadherin in the formation of a functional trophectoderm. Development. 2007;134:31-41 pubmed..Thus, N-cadherin can maintain epithelia in differentiating ES cells, but not during the formation of the trophectoderm. Our results point to a specific and unique function for E-cadherin during mouse preimplantation development. ..
- Sugihara Mizuno Y, Adachi M, Kobayashi Y, Hamazaki Y, Nishimura M, Imai T, et al. Molecular characterization of angiomotin/JEAP family proteins: interaction with MUPP1/Patj and their endogenous properties. Genes Cells. 2007;12:473-86 pubmed
- Srinivasan K, Roosa J, Olsen O, Lee S, Bredt D, McConnell S. MALS-3 regulates polarity and early neurogenesis in the developing cerebral cortex. Development. 2008;135:1781-90 pubmed publisher..Our results suggest that MALS-3 plays a role in maintaining apicobasal polarity and is required for normal neurogenesis in the developing cortex. ..
- Sheth B, Nowak R, Anderson R, Kwong W, Papenbrock T, Fleming T. Tight junction protein ZO-2 expression and relative function of ZO-1 and ZO-2 during mouse blastocyst formation. Exp Cell Res. 2008;314:3356-68 pubmed publisher..In contrast, ZO-1 knockdown, or combined ZO-1 and ZO-2 knockdown, generated a more severe inhibition of blastocoel formation indicating distinct roles for ZO proteins in blastocyst morphogenesis. ..
- Itoh M, Nagafuchi A, Yonemura S, Kitani Yasuda T, Tsukita S. The 220-kD protein colocalizing with cadherins in non-epithelial cells is identical to ZO-1, a tight junction-associated protein in epithelial cells: cDNA cloning and immunoelectron microscopy. J Cell Biol. 1993;121:491-502 pubmed..Possible functions of the 220-kD protein (ZO-1) are discussed with special reference to the molecular mechanism for adherens and tight junction formation. ..
- Woods D, Bryant P. ZO-1, DlgA and PSD-95/SAP90: homologous proteins in tight, septate and synaptic cell junctions. Mech Dev. 1993;44:85-9 pubmed
- Wang H, Ding T, Brown N, Yamamoto Y, Prince L, Reese J, et al. Zonula occludens-1 (ZO-1) is involved in morula to blastocyst transformation in the mouse. Dev Biol. 2008;318:112-25 pubmed publisher..These results provide the first evidence that ZO-1 is involved in blastocyst formation from the morula by regulating accumulation of fluid and differentiation of nonpolar blastomeres to polar trophoblast cells...
- Yu D, Marchiando A, Weber C, Raleigh D, Wang Y, Shen L, et al. MLCK-dependent exchange and actin binding region-dependent anchoring of ZO-1 regulate tight junction barrier function. Proc Natl Acad Sci U S A. 2010;107:8237-41 pubmed publisher..These data demonstrate a critical role for the ZO-1 ABR in barrier function and suggest that MLCK-dependent ZO-1 exchange is essential to this mechanism of barrier regulation. ..
- Larina O, Thorn P. Ca2+ dynamics in salivary acinar cells: distinct morphology of the acinar lumen underlies near-synchronous global Ca2+ responses. J Cell Sci. 2005;118:4131-9 pubmed..We suggest that this may be a structural adaptation in submandibular cells to support the copious amounts of fluid secreted. ..
- Roselli S, Heidet L, Sich M, Henger A, Kretzler M, Gubler M, et al. Early glomerular filtration defect and severe renal disease in podocin-deficient mice. Mol Cell Biol. 2004;24:550-60 pubmed..was modified in Nphs2-/- kidneys: the expression of the nephrin gene was downregulated, whereas that of the ZO1 and CD2AP genes appeared to be upregulated...
- Kim E, Lee Y, Kim J, Song B, Kim S, Huh J, et al. Extracellular domain of V-set and immunoglobulin domain containing 1 (VSIG1) interacts with sertoli cell membrane protein, while its PDZ-binding motif forms a complex with ZO-1. Mol Cells. 2010;30:443-8 pubmed publisher..Our findings suggest that mouse VSIG1 interacts with Sertoli cells by heterophilic adhesion via its first Ig-like domain. In addition, its cytoplasmic domain is critical for binding to ZO-1. ..
- Kirschner N, Haftek M, Niessen C, Behne M, Furuse M, Moll I, et al. CD44 regulates tight-junction assembly and barrier function. J Invest Dermatol. 2011;131:932-43 pubmed publisher..Taken together, the results reveal an important function for CD44 in the assembly and function of TJs, suggesting their involvement in the skin barrier phenotype of CD44 KO mice. ..
- Xu J, Kausalya P, Phua D, Ali S, Hossain Z, Hunziker W. Early embryonic lethality of mice lacking ZO-2, but Not ZO-3, reveals critical and nonredundant roles for individual zonula occludens proteins in mammalian development. Mol Cell Biol. 2008;28:1669-78 pubmed publisher..Thus, ZO-2 is required for mouse embryonic development, but ZO-3 is dispensable. This is to our knowledge the first report showing that an individual ZO protein plays a nonredundant and critical role in mammalian development. ..
- Yamazaki Y, Umeda K, Wada M, Nada S, Okada M, Tsukita S, et al. ZO-1- and ZO-2-dependent integration of myosin-2 to epithelial zonula adherens. Mol Biol Cell. 2008;19:3801-11 pubmed publisher..Here, we immunofluorescently showed that myosin-2 failed to be integrated into the ZA in cultured epithelial-type ZO1(ko)/2(kd) Eph4 cells lacking ZO-1 and -2 (zonula occludens-1 and -2) by knockout and knockdown, respectively...
- VandenBerg A, Sassoon D. Non-canonical Wnt signaling regulates cell polarity in female reproductive tract development via van gogh-like 2. Development. 2009;136:1559-70 pubmed publisher..These studies demonstrate that the establishment and maintenance of cell polarity through non-canonical Wnt signaling are required for FRT development. ..
- Katsuno T, Umeda K, Matsui T, Hata M, Tamura A, Itoh M, et al. Deficiency of zonula occludens-1 causes embryonic lethal phenotype associated with defected yolk sac angiogenesis and apoptosis of embryonic cells. Mol Biol Cell. 2008;19:2465-75 pubmed publisher..To investigate the role of ZO-1 (encoded by Tjp1) in vivo, ZO-1 knockout (Tjp1(-/-)) mice were generated by gene targeting...
- Hashizume A, Ueno T, Furuse M, Tsukita S, Nakanishi Y, Hieda Y. Expression patterns of claudin family of tight junction membrane proteins in developing mouse submandibular gland. Dev Dyn. 2004;231:425-31 pubmed..These results will provide a valuable resource for further investigation of tubulogenesis and physiological regulation of claudin-based tight junctions. ..
- Phillips H, Rhee H, Murdoch J, Hildreth V, Peat J, Anderson R, et al. Disruption of planar cell polarity signaling results in congenital heart defects and cardiomyopathy attributable to early cardiomyocyte disorganization. Circ Res. 2007;101:137-45 pubmed..We propose that heterozygosity for mutations in different genes in the planar cell polarity pathway may be an important mechanism for congenital heart defects and cardiomyopathy in humans. ..
- Citi S, Cordenonsi M. Tight junction proteins. Biochim Biophys Acta. 1998;1448:1-11 pubmed
- Xu J, Lim S, Ng M, Ali S, Kausalya J, Limviphuvadh V, et al. ZO-1 regulates Erk, Smad1/5/8, Smad2, and RhoA activities to modulate self-renewal and differentiation of mouse embryonic stem cells. Stem Cells. 2012;30:1885-900 pubmed publisherZO-1/Tjp1 is a cytosolic adaptor that links tight junction (TJ) transmembrane proteins to the actin cytoskeleton and has also been implicated in regulating cell proliferation and differentiation by interacting with transcriptional ..
- Umeda K, Ikenouchi J, Katahira Tayama S, Furuse K, Sasaki H, Nakayama M, et al. ZO-1 and ZO-2 independently determine where claudins are polymerized in tight-junction strand formation. Cell. 2006;126:741-54 pubmed..When NZO-1 was forcibly recruited to lateral membranes and dimerized, claudins were dramatically polymerized. These findings indicate that ZO-1 and ZO-2 can independently determine whether and where claudins are polymerized. ..
- Strumpf D, Mao C, Yamanaka Y, Ralston A, Chawengsaksophak K, Beck F, et al. Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Development. 2005;132:2093-102 pubmed..Thus, Cdx2 is essential for segregation of the ICM and TE lineages at the blastocyst stage by ensuring repression of Oct4 and Nanog in the TE. ..
- Hirose T, Karasawa M, Sugitani Y, Fujisawa M, Akimoto K, Ohno S, et al. PAR3 is essential for cyst-mediated epicardial development by establishing apical cortical domains. Development. 2006;133:1389-98 pubmed..By contrast, ZO1 and alpha4/beta1 integrins normally localize to cell-cell junctions and basal domains, respectively...
- Adachi M, Inoko A, Hata M, Furuse K, Umeda K, Itoh M, et al. Normal establishment of epithelial tight junctions in mice and cultured cells lacking expression of ZO-3, a tight-junction MAGUK protein. Mol Cell Biol. 2006;26:9003-15 pubmed..These findings indicate that ZO-3 is dispensable in vivo in terms of individual viability, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment. ..
- Colussi C, Rosati J, Straino S, Spallotta F, Berni R, Stilli D, et al. N?-lysine acetylation determines dissociation from GAP junctions and lateralization of connexin 43 in normal and dystrophic heart. Proc Natl Acad Sci U S A. 2011;108:2795-800 pubmed publisher..These results suggest that Cx43 N(?)-lysine acetylation may have physiopathological consequences for cell to cell coupling and cardiac function. ..
- Eisenhofer G, Tian H, Holmes C, Matsunaga J, Roffler Tarlov S, Hearing V. Tyrosinase: a developmentally specific major determinant of peripheral dopamine. FASEB J. 2003;17:1248-55 pubmed..The transient nature of this source of dopamine reflects a developmental switch in tyrosinase-dependent production of dopamine to production of melanin. ..
- Chattopadhyay R, Dyukova E, Singh N, Ohba M, Mobley J, Rao G. Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-hydroxyeicosatetraenoic acid partly via protein kinase C ?-mediated zona occludens-1 phosphorylation at threonine 770/772. J Biol Chem. 2014;289:3148-63 pubmed publisher
- Li X, Olson C, Lu S, Kamasawa N, Yasumura T, Rash J, et al. Neuronal connexin36 association with zonula occludens-1 protein (ZO-1) in mouse brain and interaction with the first PDZ domain of ZO-1. Eur J Neurosci. 2004;19:2132-46 pubmed..These results demonstrating a Cx36/ZO-1 association suggest a regulatory and/or scaffolding role of ZO-1 at gap junctions that form electrical synapses between neurons in mammalian brain. ..
- Ma X, Sung D, Yang Y, Wakabayashi Y, Adelstein R. Nonmuscle myosin IIB regulates epicardial integrity and epicardium-derived mesenchymal cell maturation. J Cell Sci. 2017;130:2696-2706 pubmed publisher..Our findings provide a novel mechanism linking epicardial formation and epicardial function to the activity of the cytoplasmic motor protein NMIIB. ..
- Hulander M, Kiernan A, Blomqvist S, Carlsson P, Samuelsson E, Johansson B, et al. Lack of pendrin expression leads to deafness and expansion of the endolymphatic compartment in inner ears of Foxi1 null mutant mice. Development. 2003;130:2013-25 pubmed..Thus, mutations in FOXI1 could prove to cause a Pendred syndrome-like human deafness...
- Lee S, Shin J, Sagong B, Kim U, Bok J. Spatiotemporal expression patterns of clusterin in the mouse inner ear. Cell Tissue Res. 2017;370:89-97 pubmed publisher..These specific spatiotemporal expression patterns suggest the possible roles of CLU in inner ear development and in maintaining proper hearing function. ..
- Okabe N, Shimizu K, Ozaki Kuroda K, Nakanishi H, Morimoto K, Takeuchi M, et al. Contacts between the commissural axons and the floor plate cells are mediated by nectins. Dev Biol. 2004;273:244-56 pubmed..These results indicate that the contacts between the commissural axons and the floor plate cells are mediated by the hetero-trans-interaction between nectin-1 and -3 and involved in regulation of the trajectory of the commissural axons. ..
- Schmidt A, Utepbergenov D, Krause G, Blasig I. Use of surface plasmon resonance for real-time analysis of the interaction of ZO-1 and occludin. Biochem Biophys Res Commun. 2001;288:1194-9 pubmed..We demonstrate the use of SPR spectroscopy as an effective approach for characterisation of the interactions of junction proteins. ..
- Naim E, Bernstein A, Bertram J, Caruana G. Mutagenesis of the epithelial polarity gene, discs large 1, perturbs nephrogenesis in the developing mouse kidney. Kidney Int. 2005;68:955-65 pubmed..1 and/or GUK domains of Dlg1 disrupt epithelial polarity and perturb nephrogenesis either as a secondary consequence to a defect in ureteric branching morphogenesis and/or delay in mesenchyme-to- epithelial transition. ..
- Marty Santos L, Cleaver O. Pdx1 regulates pancreas tubulogenesis and E-cadherin expression. Development. 2016;143:101-12 pubmed publisher..These findings demonstrate a novel link between regulators of epithelial architecture, specification of pancreatic cell fate and organogenesis. ..
- Prin F, Serpente P, Itasaki N, Gould A. Hox proteins drive cell segregation and non-autonomous apical remodelling during hindbrain segmentation. Development. 2014;141:1492-502 pubmed publisher..This study suggests that most, if not all, Hox proteins share a common potential to induce cell segregation but in some contexts this is masked or modulated by other transcription factors. ..
- Mellas R, Kim H, Osinski J, Sadibasic S, Gronostajski R, Cho M, et al. NFIB regulates embryonic development of submandibular glands. J Dent Res. 2015;94:312-9 pubmed publisher..These findings indicate that the transcription factor NFIB is not required for branching morphogenesis but plays a key role in tubule cell differentiation during mouse SMG development. ..
- Johansson J, Voss U, Kesavan G, Kostetskii I, Wierup N, Radice G, et al. N-cadherin is dispensable for pancreas development but required for beta-cell granule turnover. Genesis. 2010;48:374-81 pubmed publisher..The number of insulin secretory granules is significantly reduced in N-cadherin-deficient beta-cells, and as a consequence insulin secretion is decreased. ..
- Valenta T, Gay M, Steiner S, Draganova K, Zemke M, Hoffmans R, et al. Probing transcription-specific outputs of ?-catenin in vivo. Genes Dev. 2011;25:2631-43 pubmed publisher..Our model animals thus allow dissecting signaling and structural functions of ?-catenin in vivo and provide the first genetic tool to generate cells and tissues that entirely and exclusively lack canonical Wnt pathway activity. ..
- Li Y, Litingtung Y, Ten Dijke P, Chiang C. Aberrant Bmp signaling and notochord delamination in the pathogenesis of esophageal atresia. Dev Dyn. 2007;236:746-54 pubmed..Notably, ablating Bmp7 function in Nog(-/-) embryos rescued EA/TEF and notochord branching defects, establishing a critical role of Noggin-mediated Bmp7 antagonism in EA/TEF pathogenesis...
- Tan X, Yuan Y, Zeng G, Apte U, Thompson M, Cieply B, et al. Beta-catenin deletion in hepatoblasts disrupts hepatic morphogenesis and survival during mouse development. Hepatology. 2008;47:1667-79 pubmed publisher..Beta-catenin regulates multiple, critical events during the process of hepatic morphogenesis, including hepatoblast maturation, expansion, and survival, making it indispensable to survival. ..
- Jeanne M, Jorgensen J, Gould D. Molecular and Genetic Analyses of Collagen Type IV Mutant Mouse Models of Spontaneous Intracerebral Hemorrhage Identify Mechanisms for Stroke Prevention. Circulation. 2015;131:1555-65 pubmed publisher..Our data are the first to show therapeutic prevention in vivo of ICH resulting from Col4a1 mutation and imply that a mechanism-based therapy promoting protein folding might also prevent ICH in patients with COL4A1 and COL4A2 mutations. ..
- Schell C, Kretz O, Bregenzer A, Rogg M, HelmstÃ¤dter M, Lisewski U, et al. Podocyte-Specific Deletion of Murine CXADR Does Not Impair Podocyte Development, Function or Stress Response. PLoS ONE. 2015;10:e0129424 pubmed publisher..Thus these data indicate that in contrast to lower vertebrate models, mammalian podocytes have acquired molecular programs to compensate for the loss of Cxadr. ..
- Lachke S, Higgins A, Inagaki M, Saadi I, Xi Q, Long M, et al. The cell adhesion gene PVRL3 is associated with congenital ocular defects. Hum Genet. 2012;131:235-50 pubmed publisher..Collectively, these data identify PVRL3 as a critical gene involved in a Nectin-mediated cell-cell adhesion mechanism in human ocular development. ..
- Arbeille E, Reynaud F, Sanyas I, Bozon M, Kindbeiter K, Causeret F, et al. Cerebrospinal fluid-derived Semaphorin3B orients neuroepithelial cell divisions in the apicobasal axis. Nat Commun. 2015;6:6366 pubmed publisher..Thus, extrinsic control mediated by the Semaphorin signalling orients progenitor divisions in neurogenic zones. ..
- Liu L, Jiang Y, Steinle J. Toll-Like Receptor 4 Reduces Occludin and Zonula Occludens 1 to Increase Retinal Permeability Both in vitro and in vivo. J Vasc Res. 2017;54:367-375 pubmed publisher..In conclusion, these studies demonstrate that TLR4 in EC strongly regulates retinal permeability and neuronal and vascular changes following exposure to stressors such as I/R. ..
- Zhong Z, Deane R, Ali Z, Parisi M, Shapovalov Y, O Banion M, et al. ALS-causing SOD1 mutants generate vascular changes prior to motor neuron degeneration. Nat Neurosci. 2008;11:420-2 pubmed publisher..SOD1 mutant-mediated endothelial damage accumulated before motor neuron degeneration and the neurovascular inflammatory response occurred, indicating that it was a central contributor to disease initiation. ..
- Son A, Cooper M, Sheleg M, Sun Y, Kleiman N, Zhou R. Further analysis of the lens of ephrin-A5-/- mice: development of postnatal defects. Mol Vis. 2013;19:254-66 pubmed..These observations, in concert with our previous study, strongly suggest that ephrin-A5 has a critical role in postnatal lens fiber organization to maintain lens transparency. ..
- Yates L, Schnatwinkel C, Hazelwood L, Chessum L, Paudyal A, Hilton H, et al. Scribble is required for normal epithelial cell-cell contacts and lumen morphogenesis in the mammalian lung. Dev Biol. 2013;373:267-80 pubmed publisher..Thus we reveal novel and important roles for Scrib in lung development operating via the PCP pathway, and in regulating junctional complexes and cell cohesion. ..
- Soofi A, Levitan I, Dressler G. Two novel EGFP insertion alleles reveal unique aspects of Pax2 function in embryonic and adult kidneys. Dev Biol. 2012;365:241-50 pubmed publisher..These experiments provide new insight into the role of Pax2 protein levels in determining correct renal architecture and cell fate. These new Pax2 alleles are valuable genetic reagents for in vivo studies of urogenital development. ..
- Gye M, Oh Y, Lee J, Shim S, Choi K, Ahn H. Expression of coxsackievirus and adenovirus receptor isoforms in developing mouse bladder uroepithelium. Urology. 2011;77:1009.e9-1009.e18 pubmed publisher..The expression of CAR in the uroepithelial cells can be integrated as a part of the strategy for virus-mediated gene therapy in the bladder uroepithelium. ..
- D Atri F, Nadalutti F, Citi S. Evidence for a functional interaction between cingulin and ZO-1 in cultured cells. J Biol Chem. 2002;277:27757-64 pubmed
- Grisanti L, Revenkova E, Gordon R, Iomini C. Primary cilia maintain corneal epithelial homeostasis by regulation of the Notch signaling pathway. Development. 2016;143:2160-71 pubmed publisher..Thus, we have uncovered a function of the primary cilium in maintaining homeostasis of the CE by balancing proliferation and vertical migration of basal CECs through modulation of Notch signaling. ..
- Pantaleon M, Kanai Azuma M, Mattick J, Kaibuchi K, Kaye P, Wood S. FAM deubiquitylating enzyme is essential for preimplantation mouse embryo development. Mech Dev. 2001;109:151-60 pubmed..However, the nascent protein was mislocalised at the apical surface of blastomeres. Therefore FAM is required for preimplantation mouse embryo development and regulates beta-catenin and AF-6 in vivo. ..
- Ciolofan C, Lynn B, Wellershaus K, Willecke K, Nagy J. Spatial relationships of connexin36, connexin57 and zonula occludens-1 in the outer plexiform layer of mouse retina. Neuroscience. 2007;148:473-88 pubmed
- Müller S, Portwich M, Schmidt A, Utepbergenov D, Huber O, Blasig I, et al. The tight junction protein occludin and the adherens junction protein alpha-catenin share a common interaction mechanism with ZO-1. J Biol Chem. 2005;280:3747-56 pubmed
- Zemke A, Snyder J, Brockway B, Drake J, Reynolds S, Kaminski N, et al. Molecular staging of epithelial maturation using secretory cell-specific genes as markers. Am J Respir Cell Mol Biol. 2009;40:340-8 pubmed publisher..We have defined a broader repertoire of Clara cell-specific genes that allows staging of epithelial maturation during development and repair. ..
- Zhang L, Deng M, Kao C, Kao W, Xia Y. MEK kinase 1 regulates c-Jun phosphorylation in the control of corneal morphogenesis. Mol Vis. 2003;9:584-93 pubmed..Altogether, MEKK1 plays a major role in ocular surface morphogenesis and its ablation leads to damage and various eye manifestations at postnatal stages. ..