Gene Symbol: St8sia4
Description: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4
Alias: PST, PST-1, SIAT8-D, ST8SiaIV, Siat8d, CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase, alpha-2,8-sialyltransferase 8D, polysialyltransferase-1, sialyltransferase 8 (alpha-2, 8-sialyltransferase) D, sialyltransferase 8D, sialyltransferase St8Sia IV, sialytransferase St8Sia IV
Species: mouse
Products:     St8sia4

Top Publications

  1. Yoshida Y, Kojima N, Kurosawa N, Hamamoto T, Tsuji S. Molecular cloning of Sia alpha 2,3Gal beta 1,4GlcNAc alpha 2,8-sialyltransferase from mouse brain. J Biol Chem. 1995;270:14628-33 pubmed
    ..The ST8Sia III gene was expressed only in brain and testis, and it appeared first in 20 postcoitum embryonal brain and then decreased. Therefore, the new alpha 2,8-sialyltransferase is closely involved in brain development. ..
  2. Angata K, Nakayama J, Fredette B, Chong K, Ranscht B, Fukuda M. Human STX polysialyltransferase forms the embryonic form of the neural cell adhesion molecule. Tissue-specific expression, neurite outgrowth, and chromosomal localization in comparison with another polysialyltransferase, PST. J Biol Chem. 1997;272:7182-90 pubmed
    b>PST and STX are polysialyltransferases that form polysialic acid in the neural cell adhesion molecule (N-CAM), although it is not known why these two polysialyltransferases exist...
  3. Galuska S, Oltmann Norden I, Geyer H, Weinhold B, Kuchelmeister K, Hildebrandt H, et al. Polysialic acid profiles of mice expressing variant allelic combinations of the polysialyltransferases ST8SiaII and ST8SiaIV. J Biol Chem. 2006;281:31605-15 pubmed
    ..The synthesis of this unique carbohydrate polymer depends on the polysialyltransferases ST8SiaII and ST8SiaIV. Aiming to understand in more detail the contributions of ST8SiaII and ST8SiaIV to polySia biosynthesis in vivo, ..
  4. Drake P, Nathan J, Stock C, Chang P, Muench M, Nakata D, et al. Polysialic acid, a glycan with highly restricted expression, is found on human and murine leukocytes and modulates immune responses. J Immunol. 2008;181:6850-8 pubmed
    ..This is the first demonstration of polySia expression and regulation on myeloid cells, and the results in animal models suggest a role for polySia in immune regulation. ..
  5. Koutsoudaki P, Hildebrandt H, Gudi V, Skripuletz T, Skuljec J, Stangel M. Remyelination after cuprizone induced demyelination is accelerated in mice deficient in the polysialic acid synthesizing enzyme St8siaIV. Neuroscience. 2010;171:235-44 pubmed publisher
    ..The enzyme St8siaIV is one of the two polysialyltransferases responsible for PSA synthesis, and it is predominantly active during ..
  6. Oltmann Norden I, Galuska S, Hildebrandt H, Geyer R, Gerardy Schahn R, Geyer H, et al. Impact of the polysialyltransferases ST8SiaII and ST8SiaIV on polysialic acid synthesis during postnatal mouse brain development. J Biol Chem. 2008;283:1463-71 pubmed
    ..Two polysialyltransferases, ST8SiaII and ST8SiaIV, mediate polySia biosynthesis...
  7. Eckhardt M, Bukalo O, Chazal G, Wang L, Goridis C, Schachner M, et al. Mice deficient in the polysialyltransferase ST8SiaIV/PST-1 allow discrimination of the roles of neural cell adhesion molecule protein and polysialic acid in neural development and synaptic plasticity. J Neurosci. 2000;20:5234-44 pubmed
    ..We used gene-targeting to generate mice lacking ST8SiaIV/PST-1, one of the polysialyltransferases responsible for addition of polysialic acid (PSA) to NCAM...
  8. Weinhold B, Seidenfaden R, Röckle I, Muhlenhoff M, Schertzinger F, Conzelmann S, et al. Genetic ablation of polysialic acid causes severe neurodevelopmental defects rescued by deletion of the neural cell adhesion molecule. J Biol Chem. 2005;280:42971-7 pubmed
  9. Nacher J, Guirado R, Varea E, Alonso Llosa G, Röckle I, Hildebrandt H. Divergent impact of the polysialyltransferases ST8SiaII and ST8SiaIV on polysialic acid expression in immature neurons and interneurons of the adult cerebral cortex. Neuroscience. 2010;167:825-37 pubmed publisher
    ..In the brain, PSA is exclusively synthesized by the two polysialyltransferases ST8SiaII and ST8SiaIV. To study their individual contribution to polysialylation in the adult, we analyzed PSA expression in mice ..

More Information


  1. Takashima S, Yoshida Y, Kanematsu T, Kojima N, Tsuji S. Genomic structure and promoter activity of the mouse polysialic acid synthase (mST8Sia IV/PST) gene. J Biol Chem. 1998;273:7675-83 pubmed
    The mouse gene encoding ST8Sia IV/PST, one of two polysialic acid synthases, was isolated and characterized. The mST8Sia IV/PST gene was found to comprise over 60 kilobases and to be composed of five exons...
  2. Galuska S, Rollenhagen M, Kaup M, Eggers K, Oltmann Norden I, Schiff M, et al. Synaptic cell adhesion molecule SynCAM 1 is a target for polysialylation in postnatal mouse brain. Proc Natl Acad Sci U S A. 2010;107:10250-5 pubmed publisher
    ..Both polysialyltransferases, ST8SiaII and ST8SiaIV, were able to polysialylate SynCAM 1 in vitro, and polysialylation of SynCAM 1 completely abolished homophilic ..
  3. Windfuhr M, Manegold A, Muhlenhoff M, Eckhardt M, Gerardy Schahn R. Molecular defects that cause loss of polysialic acid in the complementation group 2A10. J Biol Chem. 2000;275:32861-70 pubmed
    ..PSA biosynthesis is catalyzed by two polysialyltransferases, ST8SiaII and ST8SiaIV. The catalytic mechanisms of these enzymes are unknown...
  4. Rollenhagen M, Kuckuck S, Ulm C, Hartmann M, Galuska S, Geyer R, et al. Polysialylation of the synaptic cell adhesion molecule 1 (SynCAM 1) depends exclusively on the polysialyltransferase ST8SiaII in vivo. J Biol Chem. 2012;287:35170-80 pubmed publisher
    ..Analysis of mice lacking one of the two polysialyltransferases, ST8SiaII or ST8SiaIV, revealed that polysialylation of SynCAM 1 is exclusively mediated by ST8SiaII throughout postnatal brain ..
  5. Kuhn R, Lohler J, Rennick D, Rajewsky K, Muller W. Interleukin-10-deficient mice develop chronic enterocolitis. Cell. 1993;75:263-74 pubmed
    ..These results indicate that the bowel inflammation in the mutants originates from uncontrolled immune responses stimulated by enteric antigens and that IL-10 is an essential immunoregulator in the intestinal tract. ..
  6. Jungnickel J, Brämer C, Bronzlik P, Lipokatic Takacs E, Weinhold B, Gerardy Schahn R, et al. Level and localization of polysialic acid is critical for early peripheral nerve regeneration. Mol Cell Neurosci. 2009;40:374-81 pubmed publisher
    ..Furthermore, the contribution of sialyltransferases ST8SiaII and ST8SiaIV for the up-regulation of polySia has not been studied so far...
  7. Angata K, Huckaby V, Ranscht B, Terskikh A, Marth J, Fukuda M. Polysialic acid-directed migration and differentiation of neural precursors are essential for mouse brain development. Mol Cell Biol. 2007;27:6659-68 pubmed
    Polysialic acid, which is synthesized by two polysialyltransferases, ST8SiaII and ST8SiaIV, plays an essential role in brain development by modifying the neural cell adhesion molecule (NCAM)...
  8. Wang B, Hu H, Yu B. Molecular characterization of pig ST8Sia IV--a critical gene for the formation of neural cell adhesion molecule and its response to sialic acid supplement in piglets. Nutr Neurosci. 2006;9:147-54 pubmed
    ..This pig cDNA provides a basis for uncovering the roles of ST8Sia IV during piglet development and maturation. ..
  9. Schiff M, Röckle I, Burkhardt H, Weinhold B, Hildebrandt H. Thalamocortical pathfinding defects precede degeneration of the reticular thalamic nucleus in polysialic acid-deficient mice. J Neurosci. 2011;31:1302-12 pubmed publisher
    ..Genetic ablation of the polySia-synthesizing enzymes ST8SiaII and ST8SiaIV generates polySia-negative but NCAM-positive (II(-/-)IV(-/-)) mice characterized by severe defects of major brain ..
  10. Rollenhagen M, Buettner F, Reismann M, Jirmo A, Grove M, Behrens G, et al. Polysialic acid on neuropilin-2 is exclusively synthesized by the polysialyltransferase ST8SiaIV and attached to mucin-type o-glycans located between the b2 and c domain. J Biol Chem. 2013;288:22880-92 pubmed publisher
    ..molecule (NCAM), where it is synthesized on N-glycans by either of the two polysialyltransferases, ST8SiaII or ST8SiaIV. In contrast to NCAM, little is known on the biosynthesis of polySia on NRP2...
  11. Guan F, Wang X, He F. Promotion of cell migration by neural cell adhesion molecule (NCAM) is enhanced by PSA in a polysialyltransferase-specific manner. PLoS ONE. 2015;10:e0124237 pubmed publisher
    ..We cloned the genes for NCAM-140 and for polysialyltransferases STX and PST (responsible for PSA synthesis) from normal murine mammary gland epithelial (NMuMG) cells and transfected them into ..
  12. Calandreau L, Marquez C, Bisaz R, Fantin M, Sandi C. Differential impact of polysialyltransferase ST8SiaII and ST8SiaIV knockout on social interaction and aggression. Genes Brain Behav. 2010;9:958-67 pubmed publisher
    ..required for the polysialylation of NCAM, sialyltransferase-X (St8SiaII or STX) and polysialyltransferase (ST8SiaIV or PST), in a series of tests for social behaviors...
  13. Benani A, Hryhorczuk C, Gouazé A, Fioramonti X, Brenachot X, Guissard C, et al. Food intake adaptation to dietary fat involves PSA-dependent rewiring of the arcuate melanocortin system in mice. J Neurosci. 2012;32:11970-9 pubmed publisher
    ..Furthermore, defects in the hypothalamic plasticity-driven adaptive response to HFD are obesogenic and could be involved in the development of metabolic diseases. ..
  14. Thompson M, Foley D, Colley K. The polysialyltransferases interact with sequences in two domains of the neural cell adhesion molecule to allow its polysialylation. J Biol Chem. 2013;288:7282-93 pubmed publisher
    ..cell adhesion molecule (NCAM) is the major substrate for the polysialyltransferases (polySTs), ST8SiaII/STX and ST8SiaIV/PST. The polysialylation of NCAM N-glycans decreases cell adhesion and alters signaling...
  15. Werneburg S, Mühlenhoff M, Stangel M, Hildebrandt H. Polysialic acid on SynCAM 1 in NG2 cells and on neuropilin-2 in microglia is confined to intracellular pools that are rapidly depleted upon stimulation. Glia. 2015;63:1240-55 pubmed publisher
    ..Ncam(-/-) microglia, but this fraction resided on O-glycans and was produced by the second polysialyltransferase, ST8SIA4, indicating the presence of yet another polySia carrier in microglia...
  16. Szewczyk L, Brozko N, Nagalski A, Röckle I, Werneburg S, Hildebrandt H, et al. ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons. Glia. 2017;65:34-49 pubmed publisher
    ..glia 2016;65:34-49. ..
  17. Schiff M, Weinhold B, Grothe C, Hildebrandt H. NCAM and polysialyltransferase profiles match dopaminergic marker gene expression but polysialic acid is dispensable for development of the midbrain dopamine system. J Neurochem. 2009;110:1661-73 pubmed publisher
    ..As studied by quantitative real-time RT-PCR, mRNA profiles of NCAM and the polysialyltransferases, ST8SiaII and ST8SiaIV, matched the course of tyrosine hydroxylase, dopamine transporter, nur-related factor 1, and paired-like ..
  18. Eckhardt M, Gerardy Schahn R. Genomic organization of the murine polysialyltransferase gene ST8SiaIV (PST-1). Glycobiology. 1998;8:1165-72 pubmed
    Polysialic acid (PSA) is an important regulator of cellular interactions. Two enzymes (ST8SiaII and ST8SiaIV) are capable of synthesizing PSA...
  19. Senkov O, Sun M, Weinhold B, Gerardy Schahn R, Schachner M, Dityatev A. Polysialylated neural cell adhesion molecule is involved in induction of long-term potentiation and memory acquisition and consolidation in a fear-conditioning paradigm. J Neurosci. 2006;26:10888-109898 pubmed
    ..Mice lacking the polysialyltransferase (ST8SialV/PST) responsible for attachment of PSA to NCAM in adulthood showed a mild deficit only in hippocampal contextual ..
  20. Stoenica L, Senkov O, Gerardy Schahn R, Weinhold B, Schachner M, Dityatev A. In vivo synaptic plasticity in the dentate gyrus of mice deficient in the neural cell adhesion molecule NCAM or its polysialic acid. Eur J Neurosci. 2006;23:2255-64 pubmed
    ..stem cell-derived immature granule cells, but not in mice deficient either in the NCAM glycoprotein or the enzyme ST8SiaIV/PST involved in polysialylation of NCAM in mature neurons...
  21. Potschka H, Pekcec A, Weinhold B, Gerardy Schahn R. Deficiency of neural cell adhesion molecule or its polysialylation modulates pharmacological effects of the AMPA receptor antagonist NBQX. Neuroscience. 2008;152:1093-8 pubmed publisher
    ..NCAM and PSA (female NCAM knockout mice) or being drastically reduced in the level of PSA expression (female ST8SiaIV knockout mice). Studies were carried out with the respective wildtype littermate controls...
  22. Kiermaier E, Moussion C, Veldkamp C, Gerardy Schahn R, de Vries I, Williams L, et al. Polysialylation controls dendritic cell trafficking by regulating chemokine recognition. Science. 2016;351:186-90 pubmed publisher
    ..Thus, we describe a glycosylation-mediated immune cell trafficking disorder and its mechanistic basis. ..
  23. Ong E, Nakayama J, Angata K, Reyes L, Katsuyama T, Arai Y, et al. Developmental regulation of polysialic acid synthesis in mouse directed by two polysialyltransferases, PST and STX. Glycobiology. 1998;8:415-24 pubmed
    ..It has been shown that two enzymes, polysialyltransferase, PST, and sialyltransferase X, STX, form polysialic acid on N-CAM...
  24. Takashima S, Tachida Y, Nakagawa T, Hamamoto T, Tsuji S. Quantitative analysis of expression of mouse sialyltransferase genes by competitive PCR. Biochem Biophys Res Commun. 1999;260:23-7 pubmed
    ..Using this method, we can investigate the levels of gene expression of sialyltransferases in various cell lines and various tissues of mice, and can accurately determine their expression levels. ..
  25. Bélanger M, Di Cristo G. Sensory experience differentially modulates the mRNA expression of the polysialyltransferases ST8SiaII and ST8SiaIV in postnatal mouse visual cortex. PLoS ONE. 2011;6:e24874 pubmed publisher
    ..In the brain, PSA is exclusively synthesized by the two polysialyltransferases ST8SiaII (also known as STX) and ST8SiaIV (also known as PST)...
  26. Markram K, Gerardy Schahn R, Sandi C. Selective learning and memory impairments in mice deficient for polysialylated NCAM in adulthood. Neuroscience. 2007;144:788-96 pubmed
    ..Mice were tested for their performance in the water maze and auditory fear conditioning (AFC). We report that ST8SiaIV knockout mice were impaired in spatial as well as reversal learning in the water maze...
  27. Zhang Y, Zhang X, Yeh J, Richardson P, Bo X. Engineered expression of polysialic acid enhances Purkinje cell axonal regeneration in L1/GAP-43 double transgenic mice. Eur J Neurosci. 2007;25:351-61 pubmed the cerebellum of the L1/GAP-43 transgenic mice and a lentiviral vector (LV) carrying the polysialyltransferase (PST) cDNA (LV/PST) was injected into the lesion site to transduce the cells in the glial scar...
  28. Drake P, Stock C, Nathan J, Gip P, Golden K, Weinhold B, et al. Polysialic acid governs T-cell development by regulating progenitor access to the thymus. Proc Natl Acad Sci U S A. 2009;106:11995-2000 pubmed publisher
    ..This phenotype probably reflects a decreased capacity of the ST8Sia IV(-/-) progenitors to escape from the bone marrow niche. Collectively, these results provide evidence that polySia is involved in hematopoietic development. ..
  29. Miyoshi G, Fishell G. Dynamic FoxG1 expression coordinates the integration of multipolar pyramidal neuron precursors into the cortical plate. Neuron. 2012;74:1045-58 pubmed publisher
    ..Thus, the dynamic expression of FoxG1 during migration within the intermediate zone is essential for the proper assembly of the cerebral cortex. ..
  30. Jungnickel J, Eckhardt M, Haastert Talini K, Claus P, Bronzlik P, Lipokatic Takacs E, et al. Polysialyltransferase overexpression in Schwann cells mediates different effects during peripheral nerve regeneration. Glycobiology. 2012;22:107-15 pubmed publisher
    ..Using transgenic mice expressing polysialyltransferase ST8SiaIV under control of a glial-specific (proteolipid protein, PLP) promoter (PLP-ST8SiaIV-transgenic mice), we tested ..
  31. Kröcher T, Röckle I, Diederichs U, Weinhold B, Burkhardt H, Yanagawa Y, et al. A crucial role for polysialic acid in developmental interneuron migration and the establishment of interneuron densities in the mouse prefrontal cortex. Development. 2014;141:3022-32 pubmed publisher
    ..Polysialylation of NCAM is implemented by the two polysialyltransferases (polySTs) ST8SIA2 and ST8SIA4. Dysregulation of the polySia-NCAM system and variation in ST8SIA2 has been linked to schizophrenia and other ..
  32. Kurosawa N, Yoshida Y, Kojima N, Tsuji S. Polysialic acid synthase (ST8Sia II/STX) mRNA expression in the developing mouse central nervous system. J Neurochem. 1997;69:494-503 pubmed
    ..The regional and transient expression of ST8Sia II mRNA coincides with that of PSA, suggesting that ST8Sia II is closely involved in the biosynthesis and expression of PSA in the developing mouse CNS. ..
  33. Pekcec A, Weinhold B, Gerardy Schahn R, Potschka H. Polysialic acid affects pathophysiological consequences of status epilepticus. Neuroreport. 2010;21:549-53 pubmed publisher
    ..neural cell adhesion molecule by the attachment of polysialic acid residues through the polysialyl-transferase, ST8SiaIV, regulates neuronal plasticity and affects cellular alterations in the epileptic brain...
  34. Röckle I, Hildebrandt H. Deficits of olfactory interneurons in polysialyltransferase- and NCAM-deficient mice. Dev Neurobiol. 2016;76:421-33 pubmed publisher
    ..Polysialylation of NCAM is implemented by two polysialyltransferases, ST8SIA2 and ST8SIA4, with overlapping functions...
  35. Werneburg S, Buettner F, Erben L, Mathews M, Neumann H, Mühlenhoff M, et al. Polysialylation and lipopolysaccharide-induced shedding of E-selectin ligand-1 and neuropilin-2 by microglia and THP-1 macrophages. Glia. 2016;64:1314-30 pubmed publisher
    ..Thus, shedding of polySia-ESL-1 and polySia-NRP2 after LPS-induced activation of microglia and THP-1 macrophages may constitute a mechanism for negative feedback regulation. GLIA 2016 GLIA 2016;64:1314-1330. ..
  36. Yoshida Y, Kojima N, Tsuji S. Molecular cloning and characterization of a third type of N-glycan alpha 2,8-sialyltransferase from mouse lung. J Biochem. 1995;118:658-64 pubmed
    ..ST8Sia IV can synthesize polysialic acid chain in vitro without any initiator sialytransferase. ..
  37. Zerwas M, Trouche S, Richetin K, Escudé T, Halley H, Gerardy Schahn R, et al. Environmental enrichment rescues memory in mice deficient for the polysialytransferase ST8SiaIV. Brain Struct Funct. 2016;221:1591-605 pubmed publisher
    ..One of them, ST8SiaIV (PST), is predominantly expressed during adulthood whereas the other one, ST8SiaII (STX), dominates during ..