Srsf2

Summary

Gene Symbol: Srsf2
Description: serine/arginine-rich splicing factor 2
Alias: D11Wsu175e, MRF-1, Pr264, SC35, Sfrs10, Sfrs2, serine/arginine-rich splicing factor 2, myelin regulatory factor 1, splicing component, 35 kDa, splicing factor Sc35, splicing factor, arginine/serine-rich 2 (SC-35)
Species: mouse
Products:     Srsf2

Top Publications

  1. Muralikrishna B, Dhawan J, Rangaraj N, Parnaik V. Distinct changes in intranuclear lamin A/C organization during myoblast differentiation. J Cell Sci. 2001;114:4001-11 pubmed
    ..Our results suggest that muscle cell differentiation is accompanied by regulated rearrangements in the organization of the A-type lamins. ..
  2. Tarapore P, Shinmura K, Suzuki H, Tokuyama Y, Kim S, Mayeda A, et al. Thr199 phosphorylation targets nucleophosmin to nuclear speckles and represses pre-mRNA processing. FEBS Lett. 2006;580:399-409 pubmed
    ..These findings indicate the involvement of NPM in the regulation of pre-mRNA processing, and its activity is controlled by CDK2-mediated phosphorylation on Thr(199). ..
  3. Bermingham J, Arden K, Naumova A, Sapienza C, Viars C, Fu X, et al. Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2). Genomics. 1995;29:70-9 pubmed
    ..3-q22. Recombinant inbred mapping of the mouse ASF/SF2 gene (Sfrs1 locus) and the mouse SC-35 gene (Sfrs2 locus) demonstrates that both genes are located in a part of mouse chromosome 11 that is homologous to human ..
  4. Lin S, Coutinho Mansfield G, Wang D, Pandit S, Fu X. The splicing factor SC35 has an active role in transcriptional elongation. Nat Struct Mol Biol. 2008;15:819-26 pubmed publisher
    ..transcriptional apparatus to efficiently modify nascent RNA, here we report that a well-studied splicing factor, SC35, affects transcriptional elongation in a gene-specific manner...
  5. Xiao R, Sun Y, Ding J, Lin S, Rose D, Rosenfeld M, et al. Splicing regulator SC35 is essential for genomic stability and cell proliferation during mammalian organogenesis. Mol Cell Biol. 2007;27:5393-402 pubmed
    ..We report here that the SR protein SC35 controls cell proliferation during pituitary gland development but is completely dispensable in terminal ..
  6. Colwill K, Feng L, Yeakley J, Gish G, Caceres J, Pawson T, et al. SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors. J Biol Chem. 1996;271:24569-75 pubmed
    ..These results suggest that SRPK1 and Clk/Sty may play different roles in regulating SR splicing factors, and suggest that Clk/Sty has a broader substrate specificity than SRPK1. ..
  7. Lin S, Xiao R, Sun P, Xu X, Fu X. Dephosphorylation-dependent sorting of SR splicing factors during mRNP maturation. Mol Cell. 2005;20:413-25 pubmed
    ..We demonstrate that ASF/SF2 and SC35 are each required for cell viability, but, surprisingly, the effector RS domain of ASF/SF2 is dispensable for cell ..
  8. Wang H, Xu X, Ding J, Bermingham J, Fu X. SC35 plays a role in T cell development and alternative splicing of CD45. Mol Cell. 2001;7:331-42 pubmed
    ..Here we report that Cre-mediated conditional deletion of the prototypical SR protein SC35 in the thymus causes a defect in T cell maturation...
  9. Potter G, Beaudoin G, DeRenzo C, Zarach J, Chen S, Thompson C. The hairless gene mutated in congenital hair loss disorders encodes a novel nuclear receptor corepressor. Genes Dev. 2001;15:2687-701 pubmed
    ..The discovery that Hr is a corepressor provides a molecular basis for specific hair loss syndromes in both humans and mice. ..

More Information

Publications56

  1. Ding J, Xu X, Yang D, Chu P, Dalton N, Ye Z, et al. Dilated cardiomyopathy caused by tissue-specific ablation of SC35 in the heart. EMBO J. 2004;23:885-96 pubmed
    ..Here we report that tissue-specific ablation of the splicing factor SC35 in the heart causes dilated cardiomyopathy (DCM)...
  2. Hernandez F, Perez M, Lucas J, Mata A, Bhat R, Avila J. Glycogen synthase kinase-3 plays a crucial role in tau exon 10 splicing and intranuclear distribution of SC35. Implications for Alzheimer's disease. J Biol Chem. 2004;279:3801-6 pubmed
    ..Immunofluorescence studies showed that after GSK-3 inhibition, SC35, a member of the SR family, is redistributed and enriched in nuclear speckles and colocalizes with the kinase...
  3. Kim E, Ilagan J, Liang Y, Daubner G, Lee S, Ramakrishnan A, et al. SRSF2 Mutations Contribute to Myelodysplasia by Mutant-Specific Effects on Exon Recognition. Cancer Cell. 2015;27:617-30 pubmed publisher
    ..Here we report that mutations affecting the splicing factor SRSF2 directly impair hematopoietic differentiation in vivo, which is not due to SRSF2 loss of function...
  4. Han J, Ding J, Byeon C, Kim J, Hertel K, Jeong S, et al. SR proteins induce alternative exon skipping through their activities on the flanking constitutive exons. Mol Cell Biol. 2011;31:793-802 pubmed publisher
  5. Kadri F, Pacifici M, Wilk A, Parker Struckhoff A, Del Valle L, Hauser K, et al. HIV-1-Tat Protein Inhibits SC35-mediated Tau Exon 10 Inclusion through Up-regulation of DYRK1A Kinase. J Biol Chem. 2015;290:30931-46 pubmed publisher
    ..The splicing factor SC35/SRSF2 binds to nuclear RNA and facilitates the incorporation of exon 10 in the TAU molecule...
  6. Karunakaran D, Congdon S, Guerrette T, Banday A, Lemoine C, Chhaya N, et al. The expression analysis of Sfrs10 and Celf4 during mouse retinal development. Gene Expr Patterns. 2013;13:425-36 pubmed publisher
    ..Here we report the expression of an ASF, serine-arginine rich splicing factor 10 (Sfrs10) and a mRNA translation regulation factor, CUGBP, elav like family member 4 (Celf4) in the developing mouse retina...
  7. Ji X, Zhou Y, Pandit S, Huang J, Li H, Lin C, et al. SR proteins collaborate with 7SK and promoter-associated nascent RNA to release paused polymerase. Cell. 2013;153:855-68 pubmed publisher
    ..We report that SRSF2 (also known as SC35, an SR-splicing factor) is part of the 7SK complex assembled at gene promoters and plays a ..
  8. Haque N, Buchberg A, Khalili K. Isolation and characterization of MRF-1, a brain-derived DNA-binding protein with a capacity to regulate expression of myelin basic protein gene. J Biol Chem. 1994;269:31149-56 pubmed
    ..homology in the central and the COOH-terminal regions of this protein with the previously identified splicing factor SC35. Cotransfection studies indicated that MRF-1 increases transcription of the MBP promoter in glial cells and ..
  9. Malatesta M, Caporaloni C, Gavaudan S, Rocchi M, Serafini S, Tiberi C, et al. Ultrastructural morphometrical and immunocytochemical analyses of hepatocyte nuclei from mice fed on genetically modified soybean. Cell Struct Funct. 2002;27:173-80 pubmed
    ..In conclusion, our data suggest that GM soybean intake can influence hepatocyte nuclear features in young and adult mice; however, the mechanisms responsible for such alterations remain unknown. ..
  10. Cheng Y, Luo C, Wu W, Xie Z, Fu X, Feng Y. Liver-Specific Deletion of SRSF2 Caused Acute Liver Failure and Early Death in Mice. Mol Cell Biol. 2016;36:1628-38 pubmed publisher
    ..Here, we show that mice lacking the splicing factor SRSF2 but not SRSF1 in hepatocytes have severe liver pathology and biochemical abnormalities...
  11. Cazalla D, Newton K, Caceres J. A novel SR-related protein is required for the second step of Pre-mRNA splicing. Mol Cell Biol. 2005;25:2969-80 pubmed
    ..SRrp53 also regulates alternative splicing in a concentration-dependent manner. Taken together, these results suggest that SRrp53 is a novel SR-related protein that has a role both in constitutive and in alternative splicing. ..
  12. Ferreira J, Carmo Fonseca M. The biogenesis of the coiled body during early mouse development. Development. 1995;121:601-12 pubmed
    ..Our results suggest that the embryonic 'nucleolus-like body' is a structural scaffold that nucleates independently the formation of the coiled body and the assembly of the machinery responsible for ribosome biosynthesis. ..
  13. Mardaryev A, Gdula M, Yarker J, Emelianov V, Emelianov V, Poterlowicz K, et al. p63 and Brg1 control developmentally regulated higher-order chromatin remodelling at the epidermal differentiation complex locus in epidermal progenitor cells. Development. 2014;141:101-11 pubmed publisher
    ..the locus relocates away from the nuclear periphery towards the nuclear interior into a compartment enriched in SC35-positive nuclear speckles...
  14. Ihara M, Stein P, Schultz R. UBE2I (UBC9), a SUMO-conjugating enzyme, localizes to nuclear speckles and stimulates transcription in mouse oocytes. Biol Reprod. 2008;79:906-13 pubmed publisher
    ..which were localized mainly on the nuclear membrane and in the nucleoplasm, UBE2I strikingly colocalized with SFRS2, which is a component of nuclear speckles and critical for mRNA processing...
  15. Manley J, Krainer A. A rational nomenclature for serine/arginine-rich protein splicing factors (SR proteins). Genes Dev. 2010;24:1073-4 pubmed publisher
  16. Zhang B, Arun G, Mao Y, Lazar Z, Hung G, Bhattacharjee G, et al. The lncRNA Malat1 is dispensable for mouse development but its transcription plays a cis-regulatory role in the adult. Cell Rep. 2012;2:111-23 pubmed publisher
    ..However, among a small number of genes that were dysregulated in adult Malat1 knockout mice, many were Malat1 neighboring genes, thus indicating a potential cis-regulatory role of Malat1 gene transcription. ..
  17. Saitoh N, Sakamoto C, Hagiwara M, Agredano Moreno L, Jiménez García L, Nakao M. The distribution of phosphorylated SR proteins and alternative splicing are regulated by RANBP2. Mol Biol Cell. 2012;23:1115-28 pubmed publisher
    ..This study demonstrates that the speckled distribution of phosphorylated pre-mRNA processing factors is regulated by the nucleocytoplasmic transport system in mammalian cells and that it is important for alternative splicing. ..
  18. Pandit S, Zhou Y, Shiue L, Coutinho Mansfield G, Li H, Qiu J, et al. Genome-wide analysis reveals SR protein cooperation and competition in regulated splicing. Mol Cell. 2013;50:223-35 pubmed publisher
    ..Here, we report global analyses of two prototypical SR proteins, SRSF1 (SF2/ASF) and SRSF2 (SC35), using splicing-sensitive arrays and CLIP-seq on mouse embryo fibroblasts (MEFs)...
  19. Pedrotti S, Busà R, Compagnucci C, Sette C. The RNA recognition motif protein RBM11 is a novel tissue-specific splicing regulator. Nucleic Acids Res. 2012;40:1021-32 pubmed publisher
    ..RBM11 is localized in the nucleoplasm and enriched in SRSF2-containing splicing speckles...
  20. Melko M, Nguyen L, Shaw M, Jolly L, Bardoni B, Gecz J. Loss of FMR2 further emphasizes the link between deregulation of immediate early response genes FOS and JUN and intellectual disability. Hum Mol Genet. 2013;22:2984-91 pubmed publisher
    ..These results suggest that FMR2 is an upstream regulator of FOS and JUN, and further link deregulation of the immediate early response genes to the pathology of ID- and FRAXE-associated ID in particular. ..
  21. Botti V, McNicoll F, Steiner M, Richter F, Solovyeva A, Wegener M, et al. Cellular differentiation state modulates the mRNA export activity of SR proteins. J Cell Biol. 2017;216:1993-2009 pubmed publisher
    ..As expected, SRSF2 and SRSF5 shuttle poorly in HeLa cells but surprisingly display considerable shuttling in pluripotent murine P19 ..
  22. Colla S, Ong D, Ogoti Y, Marchesini M, Mistry N, Clise Dwyer K, et al. Telomere dysfunction drives aberrant hematopoietic differentiation and myelodysplastic syndrome. Cancer Cell. 2015;27:644-57 pubmed publisher
    ..myeloid progenitor (CMP) differentiation by repressing the expression of mRNA splicing/processing genes, including SRSF2. RNA-seq analyses of telomere dysfunctional CMP identified aberrantly spliced transcripts linked to pathways ..
  23. Hahn P, Böse J, Edler S, Lengeling A. Genomic structure and expression of Jmjd6 and evolutionary analysis in the context of related JmjC domain containing proteins. BMC Genomics. 2008;9:293 pubmed publisher
    ..Finally, we discuss possibilities of the involvement of Jmjd6 and 1110005A03Rik in an antagonistic biochemical pathway. ..
  24. Leu S, Ouyang P. Spatial and temporal expression profile of pinin during mouse development. Gene Expr Patterns. 2006;6:620-31 pubmed
    ..Altogether these data provide us with a better understanding of the tissue distribution pattern of N-pnn during mouse development. ..
  25. Moen P, Johnson C, Byron M, Shopland L, de la Serna I, Imbalzano A, et al. Repositioning of muscle-specific genes relative to the periphery of SC-35 domains during skeletal myogenesis. Mol Biol Cell. 2004;15:197-206 pubmed
    ..Results demonstrate a cell type-specific reorganization of specific developmentally regulated loci relative to large domains of RNA metabolic factors, which may facilitate developmental regulation of genome expression...
  26. Abe K, Yamamoto R, Franke V, Cao M, Suzuki Y, Suzuki M, et al. The first murine zygotic transcription is promiscuous and uncoupled from splicing and 3' processing. EMBO J. 2015;34:1523-37 pubmed publisher
    ..The results also suggest that the first zygotic transcription itself is an active component of chromatin remodeling in 1-cell embryos. ..
  27. Qian W, Liang H, Shi J, Jin N, Grundke Iqbal I, Iqbal K, et al. Regulation of the alternative splicing of tau exon 10 by SC35 and Dyrk1A. Nucleic Acids Res. 2011;39:6161-71 pubmed publisher
    ..b>Splicing factor SC35, a member of the superfamily of the serine/arginine-rich (SR) proteins, promotes tau exon 10 inclusion...
  28. Berkovits B, Wang L, Guarnieri P, Wolgemuth D. The testis-specific double bromodomain-containing protein BRDT forms a complex with multiple spliceosome components and is required for mRNA splicing and 3'-UTR truncation in round spermatids. Nucleic Acids Res. 2012;40:7162-75 pubmed publisher
    ..We selected four of these genes to characterize: Srsf2, Ddx5, Hnrnpk and Tardbp...
  29. Inoue D, Abdel Wahab O. Modeling SF3B1 Mutations in Cancer: Advances, Challenges, and Opportunities. Cancer Cell. 2016;30:371-373 pubmed publisher
    ..The knockin mouse model described represents a valuable tool to dissect the effects of SF3B1 mutations on transformation, splicing, and less well-characterized functions of SF3B1. ..
  30. Yang L, Embree L, Tsai S, Hickstein D. Oncoprotein TLS interacts with serine-arginine proteins involved in RNA splicing. J Biol Chem. 1998;273:27761-4 pubmed
    ..The first SR protein is the mouse homolog of human splicing factor SC35, and the second SR member is a novel 183-amino acid protein that we term TASR (TLS-associated serine-..
  31. Komeno Y, Huang Y, Qiu J, Lin L, Xu Y, Zhou Y, et al. SRSF2 Is Essential for Hematopoiesis, and Its Myelodysplastic Syndrome-Related Mutations Dysregulate Alternative Pre-mRNA Splicing. Mol Cell Biol. 2015;35:3071-82 pubmed publisher
    ..b>SRSF2, a member of the serine/arginine-rich (SR) family of splicing factors, is one of the mutation targets associated ..
  32. Lee J, Nobumori C, Tu Y, Choi C, Yang S, Jung H, et al. Modulation of LMNA splicing as a strategy to treat prelamin A diseases. J Clin Invest. 2016;126:1592-602 pubmed publisher
    ..studies revealed that the exon 11 sequences contain binding sites for serine/arginine-rich splicing factor 2 (SRSF2), and SRSF2 knockdown lowered lamin A production in cells and in murine tissues...
  33. Ayane M, Preuss U, Kohler G, Nielsen P. A differentially expressed murine RNA encoding a protein with similarities to two types of nucleic acid binding motifs. Nucleic Acids Res. 1991;19:1273-8 pubmed
    ..In tissue culture, the expression of X16 mRNA can be induced by serum. The conserved protein motifs and expression pattern suggest that X16 could be involved in RNA processing correlating with cellular proliferation. ..
  34. Wang Q, Moore M, Adelmant G, Marto J, Silver P. PQBP1, a factor linked to intellectual disability, affects alternative splicing associated with neurite outgrowth. Genes Dev. 2013;27:615-26 pubmed publisher
    ..Our results indicate that PQBP1 can affect the AS of multiple mRNAs and indicate specific affected targets whose splice site determination may contribute to the disease phenotype in PQBP1-linked neurological disorders. ..
  35. Tripathi V, Song D, Zong X, Shevtsov S, Hearn S, Fu X, et al. SRSF1 regulates the assembly of pre-mRNA processing factors in nuclear speckles. Mol Biol Cell. 2012;23:3694-706 pubmed publisher
    ..Our results suggest that SR proteins mediate the assembly of nuclear speckles and regulate gene expression by influencing both transcriptional and posttranscriptional activities within the cell nucleus. ..
  36. Mende Y, Jakubik M, Riessland M, Schoenen F, Rossbach K, Kleinridders A, et al. Deficiency of the splicing factor Sfrs10 results in early embryonic lethality in mice and has no impact on full-length SMN/Smn splicing. Hum Mol Genet. 2010;19:2154-67 pubmed publisher
    The SR-like splicing factor SFRS10 (Htra2-beta1) is well known to influence various alternatively spliced exons without being an essential splicing factor...
  37. Ricciardi S, Kilstrup Nielsen C, Bienvenu T, Jacquette A, Landsberger N, Broccoli V. CDKL5 influences RNA splicing activity by its association to the nuclear speckle molecular machinery. Hum Mol Genet. 2009;18:4590-602 pubmed publisher
    ..These findings identify a biological process whose disregulation might affect neuronal maturation and activity in CDKL5-related disorders. ..
  38. Tominaga K, Johmura Y, Nishizuka M, Imagawa M. Fad24, a mammalian homolog of Noc3p, is a positive regulator in adipocyte differentiation. J Cell Sci. 2004;117:6217-26 pubmed
  39. Parfenov V, Pochukalina G, Davis D, Reinbold R, Scholer H, Murti K. Nuclear distribution of Oct-4 transcription factor in transcriptionally active and inactive mouse oocytes and its relation to RNA polymerase II and splicing factors. J Cell Biochem. 2003;89:720-32 pubmed
  40. Xu G, Arnaout M. WAC, a novel WW domain-containing adapter with a coiled-coil region, is colocalized with splicing factor SC35. Genomics. 2002;79:87-94 pubmed
    ..WAC existed mainly in a tyrosine-phosphorylated form. Immunofluorescence analysis colocalized WAC with SC35, the marker for pre-mRNA splicing machinery...
  41. Nie G, Li Y, Batten L, Griffiths B, Wang J, Findlay J, et al. Uterine expression of alternatively spliced mRNAs of mouse splicing factor SC35 during early pregnancy. Mol Hum Reprod. 2000;6:1131-9 pubmed
    ..One of the gene fragments identified was homologous to human SC35 splicing factor; the mouse counterpart had not then been cloned...
  42. Thoren L, Nørgaard G, Weischenfeldt J, Waage J, Jakobsen J, Damgaard I, et al. UPF2 is a critical regulator of liver development, function and regeneration. PLoS ONE. 2010;5:e11650 pubmed publisher
    ..Collectively, our data demonstrate the critical role of the NMD pathway in liver development, function and regeneration and highlights the importance of NMD for mammalian biology. ..
  43. Brower C, Veiga L, Jones R, Varshavsky A. Mouse Dfa is a repressor of TATA-box promoters and interacts with the Abt1 activator of basal transcription. J Biol Chem. 2010;285:17218-34 pubmed publisher
    ..Given these results, RNA interference was used to probe the influence of Dfa levels in luciferase reporter assays. We found that Dfa(A) acts as a repressor of TATA-box transcriptional promoters. ..
  44. Derlig K, Giessl A, Brandstätter J, Enz R, Dahlhaus R. Special characteristics of the transcription and splicing machinery in photoreceptor cells of the mammalian retina. Cell Tissue Res. 2015;362:281-94 pubmed publisher
  45. Lee S, Dvinge H, Kim E, Cho H, Micol J, Chung Y, et al. Modulation of splicing catalysis for therapeutic targeting of leukemia with mutations in genes encoding spliceosomal proteins. Nat Med. 2016;22:672-8 pubmed publisher
    ..To test this hypothesis, we engineered mice to express a mutated allele of serine/arginine-rich splicing factor 2 (Srsf2(P95H))-which commonly occurs in individuals with MDS and AML-in an inducible, hemizygous manner in hematopoietic ..
  46. Rivera Serrano E, Fritch E, Scholl E, Sherry B. A Cytoplasmic RNA Virus Alters the Function of the Cell Splicing Protein SRSF2. J Virol. 2017;91: pubmed publisher
    ..that T1L, but not T3D, μ2 localizes to nuclear speckles, where it forms a complex with the mRNA splicing factor SRSF2 and alters its subnuclear localization...
  47. Yan C, Tamm I. Molecular cloning and characterization of additional factors that bind to the interferon-alpha/beta response element B of the murine (2'-5')oligoadenylate synthetase ME-12 gene. Proc Natl Acad Sci U S A. 1992;89:8859-63 pubmed
    ..IREBF-2 shares marked similarity with some gene products of the herpes group of viruses. These gene products are potent transcription activators. IREBF-2 is constitutively expressed in BALB/c 3T3 cells. ..