Gene Symbol: Srsf1
Description: serine/arginine-rich splicing factor 1
Alias: 1110054N12Rik, 5730507C05Rik, 6330415C05Rik, AI482334, AW491331, Asf, Sf2, Sfrs1, serine/arginine-rich splicing factor 1, ASF/SF2, pre-mRNA-splicing factor SRp30a, splicing factor, arginine/serine-rich 1 (ASF/SF2)
Species: mouse
Products:     Srsf1

Top Publications

  1. Bernard D, Prasanth K, Tripathi V, Colasse S, Nakamura T, Xuan Z, et al. A long nuclear-retained non-coding RNA regulates synaptogenesis by modulating gene expression. EMBO J. 2010;29:3082-93 pubmed publisher
    ..Our results suggest that Malat1 regulates synapse formation by modulating the expression of genes involved in synapse formation and/or maintenance. ..
  2. Sun D, Novotny M, Bulek K, Liu C, Li X, Hamilton T. Treatment with IL-17 prolongs the half-life of chemokine CXCL1 mRNA via the adaptor TRAF5 and the splicing-regulatory factor SF2 (ASF). Nat Immunol. 2011;12:853-60 pubmed publisher
    ..other mRNAs through a pathway that involved the adaptor Act1, the adaptors TRAF2 or TRAF5 and the splicing factor SF2 (also known as alternative splicing factor (ASF))...
  3. Lin S, Xiao R, Sun P, Xu X, Fu X. Dephosphorylation-dependent sorting of SR splicing factors during mRNP maturation. Mol Cell. 2005;20:413-25 pubmed
    ..We demonstrate that ASF/SF2 and SC35 are each required for cell viability, but, surprisingly, the effector RS domain of ASF/SF2 is dispensable ..
  4. Bermingham J, Arden K, Naumova A, Sapienza C, Viars C, Fu X, et al. Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2). Genomics. 1995;29:70-9 pubmed
    ..Somatic cell hybrid mapping of the human ASF/SF2 gene (SFRS1 locus) reveals that it resides on chromosome 17, and fluorescence in situ hybridization refines this localization ..
  5. Houlard M, Artus J, Léguillier T, Vandormael Pournin S, Cohen Tannoudji M. DNA-RNA hybrids contribute to the replication dependent genomic instability induced by Omcg1 deficiency. Cell Cycle. 2011;10:108-17 pubmed
  6. Colwill K, Feng L, Yeakley J, Gish G, Caceres J, Pawson T, et al. SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors. J Biol Chem. 1996;271:24569-75 pubmed
    ..two kinases phosphorylate the same SR proteins in vitro, but that SRPK1 has the higher specific activity toward ASF/SF2. SRPK1, like Clk/Sty, phosphorylates ASF/SF2 in vitro on sites that are also phosphorylated in vivo...
  7. Vecerova J, Koberna K, Malinsky J, Soutoglou E, Sullivan T, Stewart C, et al. Formation of nuclear splicing factor compartments is independent of lamins A/C. Mol Biol Cell. 2004;15:4904-10 pubmed
    ..positional stability of splicing factor compartments is maintained and that the exchange dynamics of SF2/ASF between the compartments and the nucleoplasm is not affected by loss of lamin A/C...
  8. Ji X, Zhou Y, Pandit S, Huang J, Li H, Lin C, et al. SR proteins collaborate with 7SK and promoter-associated nascent RNA to release paused polymerase. Cell. 2013;153:855-68 pubmed publisher
    ..These findings reveal an unanticipated SR protein function, a role for promoter-proximal nascent RNA in gene activation, and an analogous mechanism to HIV Tat/TAR for activating cellular genes. ..
  9. Xu X, Yang D, Ding J, Wang W, Chu P, Dalton N, et al. ASF/SF2-regulated CaMKIIdelta alternative splicing temporally reprograms excitation-contraction coupling in cardiac muscle. Cell. 2005;120:59-72 pubmed
    ..We identify the essential splicing factor ASF/SF2 as a key component of the program, regulating a restricted set of tissue-specific alternative splicing events ..

More Information


  1. Cazalla D, Newton K, Caceres J. A novel SR-related protein is required for the second step of Pre-mRNA splicing. Mol Cell Biol. 2005;25:2969-80 pubmed
    ..SRrp53 also regulates alternative splicing in a concentration-dependent manner. Taken together, these results suggest that SRrp53 is a novel SR-related protein that has a role both in constitutive and in alternative splicing. ..
  2. Shimoni Sebag A, Lebenthal Loinger I, Zender L, Karni R. RRM1 domain of the splicing oncoprotein SRSF1 is required for MEK1-MAPK-ERK activation and cellular transformation. Carcinogenesis. 2013;34:2498-504 pubmed publisher
    ..We have shown previously that the splicing factor serine and arginine splicing factor 1 (SRSF1; SF2/ASF) is a proto-oncogene, which is amplified in breast cancer and transforms immortal cells when ..
  3. Kim T, Kim J, Oh J, Hong S, Kim D. Pressure-overload cardiac hypertrophy is associated with distinct alternative splicing due to altered expression of splicing factors. Mol Cells. 2014;37:81-7 pubmed publisher
    ..We found, through Western blotting, that the expression levels of three splicing factors, ESRP1, PTB and SF2/ASF, were significantly altered during cardiac hypertrophy...
  4. Xie N, Chen M, Dai R, Zhang Y, Zhao H, Song Z, et al. SRSF1 promotes vascular smooth muscle cell proliferation through a Δ133p53/EGR1/KLF5 pathway. Nat Commun. 2017;8:16016 pubmed publisher
    ..Here we report that SRSF1 (serine/arginine-rich splicing factor 1), an essential splicing factor, promotes VSMC proliferation and injury-..
  5. Tacke R, Boned A, Goridis C. ASF alternative transcripts are highly conserved between mouse and man. Nucleic Acids Res. 1992;20:5482 pubmed
  6. Kanadia R, Clark V, Punzo C, Trimarchi J, Cepko C. Temporal requirement of the alternative-splicing factor Sfrs1 for the survival of retinal neurons. Development. 2008;135:3923-33 pubmed publisher
    ..We have investigated the role of the alternative-splicing factor Sfrs1, an arginine/serine-rich (SR) protein family member, during mouse retinal development...
  7. Ayane M, Preuss U, Kohler G, Nielsen P. A differentially expressed murine RNA encoding a protein with similarities to two types of nucleic acid binding motifs. Nucleic Acids Res. 1991;19:1273-8 pubmed
    ..In tissue culture, the expression of X16 mRNA can be induced by serum. The conserved protein motifs and expression pattern suggest that X16 could be involved in RNA processing correlating with cellular proliferation. ..
  8. Zhong B, Liu X, Wang X, Chang S, Liu X, Wang A, et al. Negative regulation of IL-17-mediated signaling and inflammation by the ubiquitin-specific protease USP25. Nat Immunol. 2012;13:1110-7 pubmed publisher
    ..Thus, our results demonstrate that USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling. ..
  9. Verduci L, Simili M, Rizzo M, Mercatanti A, Evangelista M, Mariani L, et al. MicroRNA (miRNA)-mediated interaction between leukemia/lymphoma-related factor (LRF) and alternative splicing factor/splicing factor 2 (ASF/SF2) affects mouse embryonic fibroblast senescence and apoptosis. J Biol Chem. 2010;285:39551-63 pubmed publisher
    ..Both miRNAs are predicted to target alternative splicing factor/splicing factor 2 (ASF/SF2), a serine/arginine protein essential for cell viability...
  10. Manley J, Krainer A. A rational nomenclature for serine/arginine-rich protein splicing factors (SR proteins). Genes Dev. 2010;24:1073-4 pubmed publisher
  11. Han J, Ding J, Byeon C, Kim J, Hertel K, Jeong S, et al. SR proteins induce alternative exon skipping through their activities on the flanking constitutive exons. Mol Cell Biol. 2011;31:793-802 pubmed publisher
    ..We previously characterized SRSF1 (SF2/ASF)-dependent exon skipping of the CaMKII? gene during heart remodeling...
  12. Huang S, Cho A, Norton S, Liu E, Park J, Zhou A, et al. Coupled transcription-splicing regulation of mutually exclusive splicing events at the 5' exons of protein 4.1R gene. Blood. 2009;114:4233-42 pubmed publisher
    ..Furthermore, splicing factor SF2/ASF ultraviolet (UV) cross-linked to the exon 2'/2 junction CAGAGAA, a sequence that overlaps the distal U2AF(35)-..
  13. Tripathi V, Song D, Zong X, Shevtsov S, Hearn S, Fu X, et al. SRSF1 regulates the assembly of pre-mRNA processing factors in nuclear speckles. Mol Biol Cell. 2012;23:3694-706 pubmed publisher
    ..Depletion of SRSF1 in human cells compromises the association of splicing factors to nuclear speckles and influences the levels and ..
  14. Cartegni L, Krainer A. Disruption of an SF2/ASF-dependent exonic splicing enhancer in SMN2 causes spinal muscular atrophy in the absence of SMN1. Nat Genet. 2002;30:377-84 pubmed
    ..change occurs within a heptamer motif of an exonic splicing enhancer, which in SMN1 is recognized directly by SF2/ASF. The abrogation of the SF2/ASF-dependent ESE is the basis for inefficient inclusion of exon 7 in SMN2, resulting in ..
  15. Delestienne N, Wauquier C, Soin R, Dierick J, Gueydan C, Kruys V. The splicing factor ASF/SF2 is associated with TIA-1-related/TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression. FEBS J. 2010;277:2496-514 pubmed publisher
    ..Amongst the identified proteins, we found the splicing factor ASF/SF2, which is also present in TIA-1 protein complexes...
  16. Pedrotti S, Busà R, Compagnucci C, Sette C. The RNA recognition motif protein RBM11 is a novel tissue-specific splicing regulator. Nucleic Acids Res. 2012;40:1021-32 pubmed publisher
    ..of alternative 5' splice sites in BCL-X by binding to specific sequences in exon 2 and antagonizing the SR protein SRSF1. Thus, our findings identify RBM11 as a novel tissue-specific splicing factor with potential implication in the ..
  17. Cheng Y, Luo C, Wu W, Xie Z, Fu X, Feng Y. Liver-Specific Deletion of SRSF2 Caused Acute Liver Failure and Early Death in Mice. Mol Cell Biol. 2016;36:1628-38 pubmed publisher
    ..Here, we show that mice lacking the splicing factor SRSF2 but not SRSF1 in hepatocytes have severe liver pathology and biochemical abnormalities...
  18. Chen Z, Ma X, Zhang J, Hu J, Gorczynski R. Alternative splicing of CD200 is regulated by an exonic splicing enhancer and SF2/ASF. Nucleic Acids Res. 2010;38:6684-96 pubmed publisher
    ..splicing enhancer (ESE) located in exon 2, which is a putative binding site for a splicing regulatory protein SF2/ASF. Deletion or mutation of the ESE site decreased expression of the full-length CD200...
  19. Royce Tolland M, Andersen A, Koyfman H, Talbot D, Wutz A, Tonks I, et al. The A-repeat links ASF/SF2-dependent Xist RNA processing with random choice during X inactivation. Nat Struct Mol Biol. 2010;17:948-54 pubmed publisher
    ..We propose that modulation of Xist RNA processing may be part of the stochastic process that determines which X chromosome will be inactivated. ..
  20. Zerbe L, Pino I, Pio R, Cosper P, Dwyer Nield L, Meyer A, et al. Relative amounts of antagonistic splicing factors, hnRNP A1 and ASF/SF2, change during neoplastic lung growth: implications for pre-mRNA processing. Mol Carcinog. 2004;41:187-96 pubmed
    ..Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 and alternative splicing factor/splicing factor 2 (ASF/SF2) act antagonistically to modulate splice site selection...
  21. Colwill K, Pawson T, Andrews B, Prasad J, Manley J, Bell J, et al. The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution. EMBO J. 1996;15:265-75 pubmed
    ..In vitro, Clk/Sty efficiently phosphorylated the SR family member ASF/SF2 on serine residues located within its serine/arginine-rich region (the RS domain)...
  22. Hulse R, Drake R, Bates D, Donaldson L. The control of alternative splicing by SRSF1 in myelinated afferents contributes to the development of neuropathic pain. Neurobiol Dis. 2016;96:186-200 pubmed publisher
    ..that control of alternative pre-mRNA splicing, through the splice factor serine-arginine splice factor 1 (SRSF1), is integral to the processing of nociceptive information in the spinal cord...
  23. Lee M, Dworkin A, Gildea D, Trivedi N, Moorhead G, Crawford N. RRP1B is a metastasis modifier that regulates the expression of alternative mRNA isoforms through interactions with SRSF1. Oncogene. 2014;33:1818-27 pubmed publisher
    ..One such target is SRSF1 (serine/arginine-rich splicing factor 1) (SF2/ASF, splicing factor 2/alternative splicing factor), an essential ..
  24. Pandit S, Zhou Y, Shiue L, Coutinho Mansfield G, Li H, Qiu J, et al. Genome-wide analysis reveals SR protein cooperation and competition in regulated splicing. Mol Cell. 2013;50:223-35 pubmed publisher
    ..Here, we report global analyses of two prototypical SR proteins, SRSF1 (SF2/ASF) and SRSF2 (SC35), using splicing-sensitive arrays and CLIP-seq on mouse embryo fibroblasts (MEFs)...
  25. Hatakeyama S, Sugihara K, Nakayama J, Akama T, Wong S, Kawashima H, et al. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Proc Natl Acad Sci U S A. 2009;106:3095-100 pubmed publisher
    ..affinity chromatography were identified by proteomics as Ser/Arg-rich alternative pre-mRNA splicing factors or Sfrs1, Sfrs2, Sfrs5, and Sfrs7 gene products...
  26. Irwin S, Vandelft M, Pinchev D, Howell J, Graczyk J, Orr H, et al. RNA association and nucleocytoplasmic shuttling by ataxin-1. J Cell Sci. 2005;118:233-42 pubmed
    ..These results suggest that the normal role of ataxin-1 may be in RNA processing, perhaps nuclear RNA export. Thus, nuclear retention of mutant ataxin-1 may be an important toxic gain of function in SCA1 disease. ..
  27. Fregoso O, Das S, Akerman M, Krainer A. Splicing-factor oncoprotein SRSF1 stabilizes p53 via RPL5 and induces cellular senescence. Mol Cell. 2013;50:56-66 pubmed publisher
    ..Therefore, the cell has many safeguards against such misregulation. We report that the oncogenic splicing factor SRSF1, which is overexpressed in many cancers, stabilizes the tumor suppressor protein p53 by abrogating its MDM2-..