Gene Symbol: Sp7
Description: Sp7 transcription factor 7
Alias: 6430578P22Rik, C22, Osx, transcription factor Sp7, zinc finger protein osterix
Species: mouse
Products:     Sp7

Top Publications

  1. Nishio Y, Dong Y, Paris M, O Keefe R, Schwarz E, Drissi H. Runx2-mediated regulation of the zinc finger Osterix/Sp7 gene. Gene. 2006;372:62-70 pubmed
    The zinc finger transcription factor Osterix (Osx) regulates bone formation and osteoblast differentiation in vitro and in vivo...
  2. Zhou X, Zhang Z, Feng J, Dusevich V, Sinha K, Zhang H, et al. Multiple functions of Osterix are required for bone growth and homeostasis in postnatal mice. Proc Natl Acad Sci U S A. 2010;107:12919-24 pubmed publisher
    The transcription factor Osterix (Osx) is required for osteoblast differentiation and bone formation during embryonic development, but it is not known whether Osx has an essential function in postnatal bone growth and in bone homeostasis...
  3. Zhang C, Cho K, Huang Y, Lyons J, Zhou X, Sinha K, et al. Inhibition of Wnt signaling by the osteoblast-specific transcription factor Osterix. Proc Natl Acad Sci U S A. 2008;105:6936-41 pubmed publisher
    ..Here, we report that the osteoblast-specific transcription factor Osterix (Osx), which is required for osteoblast differentiation, inhibits Wnt pathway activity...
  4. Yang F, Tang W, So S, de Crombrugghe B, Zhang C. Sclerostin is a direct target of osteoblast-specific transcription factor osterix. Biochem Biophys Res Commun. 2010;400:684-8 pubmed publisher
    Osterix (Osx) is an osteoblast-specific transcription factor required for osteoblast differentiation and bone formation. Osx knock-out mice lack bone completely...
  5. Koga T, Matsui Y, Asagiri M, Kodama T, de Crombrugghe B, Nakashima K, et al. NFAT and Osterix cooperatively regulate bone formation. Nat Med. 2005;11:880-5 pubmed
    ..These results may provide important insight into the management of post-transplantation osteoporosis as well as a new strategy for promoting bone regeneration in osteopenic disease. ..
  6. Nishimura R, Wakabayashi M, Hata K, Matsubara T, Honma S, Wakisaka S, et al. Osterix regulates calcification and degradation of chondrogenic matrices through matrix metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2 during endochondral ossification. J Biol Chem. 2012;287:33179-90 pubmed
  7. Tao J, Chen S, Yang T, Dawson B, Munivez E, Bertin T, et al. Osteosclerosis owing to Notch gain of function is solely Rbpj-dependent. J Bone Miner Res. 2010;25:2175-83 pubmed publisher
    ..As such, it identifies Rbpj as a specific target for manipulating Notch signaling in a cell-autonomous fashion in osteoblasts in bone diseases where Notch may be dysregulated...
  8. Zhang C, Tang W, Li Y, Yang F, Dowd D, MacDonald P. Osteoblast-specific transcription factor Osterix increases vitamin D receptor gene expression in osteoblasts. PLoS ONE. 2011;6:e26504 pubmed publisher
    Osterix (Osx) is an osteoblast-specific transcription factor required for osteoblast differentiation from mesenchymal stem cells. In Osx knock-out mice, no bone formation occurs...
  9. Ulsamer A, Ortuño M, Ruiz S, Susperregui A, Osses N, Rosa J, et al. BMP-2 induces Osterix expression through up-regulation of Dlx5 and its phosphorylation by p38. J Biol Chem. 2008;283:3816-26 pubmed
    ..Thus, we propose that BMP activates expression of Osterix through the induction of Dlx5 and its further transcriptional activation by p38-mediated phosphorylation. ..

More Information


  1. Park J, Baek W, Kim Y, Kim J. In vivo expression of Osterix in mature granule cells of adult mouse olfactory bulb. Biochem Biophys Res Commun. 2011;407:842-7 pubmed publisher
    Osterix (Osx) has been identified as an osteoblast-specific transcription factor that is required for skeletogenesis. Here, we examined the expression of Osx in non-skeletal tissues...
  2. Lu X, Gilbert L, He X, Rubin J, Nanes M. Transcriptional regulation of the osterix (Osx, Sp7) promoter by tumor necrosis factor identifies disparate effects of mitogen-activated protein kinase and NF kappa B pathways. J Biol Chem. 2006;281:6297-306 pubmed
    ..Because OB differentiation requires the expression of the transcription factor osterix (Osx), we investigated TNF effects on Osx...
  3. Nakashima K, de Crombrugghe B. Transcriptional mechanisms in osteoblast differentiation and bone formation. Trends Genet. 2003;19:458-66 pubmed
    ..Runx2 and Osx, a newly identified zinc-finger-containing protein, are transcription factors that are expressed selectively and at ..
  4. Cao Z, Zhang H, Zhou X, Han X, Ren Y, Gao T, et al. Genetic evidence for the vital function of Osterix in cementogenesis. J Bone Miner Res. 2012;27:1080-92 pubmed publisher
    ..Osterix (OSX) is a transcriptional factor essential for osteogenesis, but its role in cementogenesis has not been addressed...
  5. Hu H, Hilton M, Tu X, Yu K, Ornitz D, Long F. Sequential roles of Hedgehog and Wnt signaling in osteoblast development. Development. 2005;132:49-60 pubmed
    ..Finally Wnt7b is identified as a potential endogenous ligand regulating osteogenesis. These data support a model that integrates Hh and Wnt signaling in the regulation of osteoblast development. ..
  6. Wang X, Goh C, Li B. p38 mitogen-activated protein kinase regulates osteoblast differentiation through osterix. Endocrinology. 2007;148:1629-37 pubmed
    ..Here we provide evidence that the effect of p38 MAPK on osteoblast differentiation can be mediated by osterix (Osx), a transcription factor necessary and sufficient for osteoblast differentiation...
  7. Baek W, Lee M, Jung J, Kim S, Akiyama H, de Crombrugghe B, et al. Positive regulation of adult bone formation by osteoblast-specific transcription factor osterix. J Bone Miner Res. 2009;24:1055-65 pubmed publisher
    Osterix (Osx) is essential for osteoblast differentiation and bone formation, because mice lacking Osx die within 1 h of birth with a complete absence of intramembranous and endochondral bone formation...
  8. Tu Q, Valverde P, Chen J. Osterix enhances proliferation and osteogenic potential of bone marrow stromal cells. Biochem Biophys Res Commun. 2006;341:1257-65 pubmed
    Osterix (Osx) is a zinc-finger-containing transcription factor that is expressed in osteoblasts of all endochondral and membranous bones. In Osx null mice osteoblast differentiation is impaired and bone formation is absent...
  9. Shibata S, Yokohama Tamaki T. An in situ hybridization study of Runx2, Osterix, and Sox9 in the anlagen of mouse mandibular condylar cartilage in the early stages of embryogenesis. J Anat. 2008;213:274-83 pubmed publisher
  10. Dobreva G, Chahrour M, Dautzenberg M, Chirivella L, Kanzler B, Farinas I, et al. SATB2 is a multifunctional determinant of craniofacial patterning and osteoblast differentiation. Cell. 2006;125:971-86 pubmed
    ..Thus, SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation...
  11. Sinha K, Yasuda H, Coombes M, Dent S, de Crombrugghe B. Regulation of the osteoblast-specific transcription factor Osterix by NO66, a Jumonji family histone demethylase. EMBO J. 2010;29:68-79 pubmed publisher
    Osterix (Osx) is an osteoblast-specific transcription factor required for osteoblast differentiation and bone formation...
  12. Akiyama H, Kim J, Nakashima K, Balmes G, Iwai N, Deng J, et al. Osteo-chondroprogenitor cells are derived from Sox9 expressing precursors. Proc Natl Acad Sci U S A. 2005;102:14665-70 pubmed
    ..Thus, our results strongly suggest that all osteo-chondroprogenitor cells, as well as progenitors in a variety of tissues, are derived from Sox9-expressing precursors during mouse embryogenesis. ..
  13. Matsubara T, Kida K, Yamaguchi A, Hata K, Ichida F, Meguro H, et al. BMP2 regulates Osterix through Msx2 and Runx2 during osteoblast differentiation. J Biol Chem. 2008;283:29119-25 pubmed publisher
    Osterix/Sp7, a member of the Sp1 transcription factor family, plays an essential role in bone formation and osteoblastogenesis...
  14. Yang L, Cheng P, Chen C, He H, Xie G, Zhou H, et al. miR-93/Sp7 function loop mediates osteoblast mineralization. J Bone Miner Res. 2012;27:1598-606 pubmed publisher
    ..Expression of the Sp7 transcription factor 7 (Sp7, Osterix), a zinc finger transcription factor and critical regulator of osteoblast ..
  15. Hilton M, Tu X, Wu X, Bai S, Zhao H, Kobayashi T, et al. Notch signaling maintains bone marrow mesenchymal progenitors by suppressing osteoblast differentiation. Nat Med. 2008;14:306-14 pubmed publisher
    ..Thus, mesenchymal progenitors may be expanded in vitro by activating the Notch pathway, whereas bone formation in vivo may be enhanced by transiently suppressing this pathway. ..
  16. Hill T, Später D, Taketo M, Birchmeier W, Hartmann C. Canonical Wnt/beta-catenin signaling prevents osteoblasts from differentiating into chondrocytes. Dev Cell. 2005;8:727-38 pubmed
    ..Thus, canonical Wnt/beta-catenin signaling is essential for skeletal lineage differentiation, preventing transdifferentiation of osteoblastic cells into chondrocytes. ..
  17. Baek W, de Crombrugghe B, Kim J. Postnatally induced inactivation of Osterix in osteoblasts results in the reduction of bone formation and maintenance. Bone. 2010;46:920-8 pubmed publisher
    Osterix (Osx) is a zinc-finger-containing transcription factor that is highly specific to osteoblasts in vivo...
  18. Tu X, Joeng K, Nakayama K, Nakayama K, Rajagopal J, Carroll T, et al. Noncanonical Wnt signaling through G protein-linked PKCdelta activation promotes bone formation. Dev Cell. 2007;12:113-27 pubmed
    ..Together, these results reveal a Wnt-dependent osteogenic mechanism, and they provide a potential target pathway for designing therapeutics to promote bone formation. ..
  19. Kim Y, Kim H, Park E, Lee B, Ryoo H, Kim S, et al. The bone-related Zn finger transcription factor Osterix promotes proliferation of mesenchymal cells. Gene. 2006;366:145-51 pubmed
    ..We propose that regulatory pathways operating upstream of or in parallel with Osterix are required for osteogenic conversion of uncommitted mesenchymal cells. ..
  20. Joeng K, Long F. The Gli2 transcriptional activator is a crucial effector for Ihh signaling in osteoblast development and cartilage vascularization. Development. 2009;136:4177-85 pubmed publisher
    ..These results therefore provide direct genetic evidence that Gli2 and Gli3 collectively mediate all major aspects of Ihh function during endochondral skeletal development. ..
  21. Nakashima K, Zhou X, Kunkel G, Zhang Z, Deng J, Behringer R, et al. The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell. 2002;108:17-29 pubmed
    We have identified a novel zinc finger-containing transcription factor, called Osterix (Osx), that is specifically expressed in all developing bones. In Osx null mice, no bone formation occurs...
  22. Zhang X, Ting K, Bessette C, Culiat C, Sung S, Lee H, et al. Nell-1, a key functional mediator of Runx2, partially rescues calvarial defects in Runx2(+/-) mice. J Bone Miner Res. 2011;26:777-91 pubmed publisher
  23. Tang W, Li Y, Osimiri L, Zhang C. Osteoblast-specific transcription factor Osterix (Osx) is an upstream regulator of Satb2 during bone formation. J Biol Chem. 2011;286:32995-3002 pubmed publisher
    Osterix (Osx) is an osteoblast-specific transcription factor essential for osteoblast differentiation and bone formation. Osx knock-out mice lack bone completely...
  24. Joeng K, Schumacher C, Zylstra Diegel C, Long F, Williams B. Lrp5 and Lrp6 redundantly control skeletal development in the mouse embryo. Dev Biol. 2011;359:222-9 pubmed publisher
    ..Thus, Lrp5 and 6 redundantly control embryonic skeletal development, likely through ?-catenin signaling. ..
  25. Tang W, Yang F, Li Y, de Crombrugghe B, Jiao H, Xiao G, et al. Transcriptional regulation of Vascular Endothelial Growth Factor (VEGF) by osteoblast-specific transcription factor Osterix (Osx) in osteoblasts. J Biol Chem. 2012;287:1671-8 pubmed publisher
    Osterix (Osx) is an osteoblast-specific transcription factor required for bone formation and osteoblast differentiation. The critical step in bone formation is to replace the avascular cartilage template with vascularized bone...
  26. Yadav V, Ryu J, Suda N, Tanaka K, Gingrich J, Schutz G, et al. Lrp5 controls bone formation by inhibiting serotonin synthesis in the duodenum. Cell. 2008;135:825-37 pubmed publisher
    ..By identifying duodenum-derived serotonin as a hormone inhibiting bone formation in an Lrp5-dependent manner, this study broadens our understanding of bone remodeling and suggests potential therapies to increase bone mass. ..
  27. Tominaga H, Maeda S, Miyoshi H, Miyazono K, Komiya S, Imamura T. Expression of osterix inhibits bone morphogenetic protein-induced chondrogenic differentiation of mesenchymal progenitor cells. J Bone Miner Metab. 2009;27:36-45 pubmed publisher
    ..These results suggest that the low levels of osterix expression remaining after knockdown are sufficient to block chondrogenesis, whereas higher expression may be required to promote osteoblastic differentiation. ..
  28. Kaback L, Soung D, Naik A, Smith N, Schwarz E, O Keefe R, et al. Osterix/Sp7 regulates mesenchymal stem cell mediated endochondral ossification. J Cell Physiol. 2008;214:173-82 pubmed
    We investigated the expression and regulation of the zinc finger protein Osterix (Osx) during endochondral ossification in mice...
  29. Cao Y, Jia S, Chakravarty G, de Crombrugghe B, Kleinerman E. The osterix transcription factor down-regulates interleukin-1 alpha expression in mouse osteosarcoma cells. Mol Cancer Res. 2008;6:119-26 pubmed publisher
    K7M2 mouse osteosarcoma cells form lytic tumors and are deficient in osterix (Osx), a zinc finger-containing transcription factor required for osteoblast differentiation and bone formation...
  30. Yang L, Tsang K, Tang H, Chan D, Cheah K. Hypertrophic chondrocytes can become osteoblasts and osteocytes in endochondral bone formation. Proc Natl Acad Sci U S A. 2014;111:12097-102 pubmed publisher
  31. Guo X, Mak K, Taketo M, Yang Y. The Wnt/beta-catenin pathway interacts differentially with PTHrP signaling to control chondrocyte hypertrophy and final maturation. PLoS ONE. 2009;4:e6067 pubmed publisher
    ..Furthermore, Wnt/beta-catenin signaling also controls final maturation of hypertrophic chondrocytes, but such regulation is PTHrP signaling-independent. ..
  32. Ehlen H, Chinenkova M, Moser M, Munter H, Krause Y, Gross S, et al. Inactivation of anoctamin-6/Tmem16f, a regulator of phosphatidylserine scrambling in osteoblasts, leads to decreased mineral deposition in skeletal tissues. J Bone Miner Res. 2013;28:246-59 pubmed publisher
    ..Our study is the first to our knowledge to reveal the requirement of Ano6 in PS scrambling in osteoblasts, supporting a function of PS exposure in the deposition of hydroxyapatite. ..
  33. Gámez B, Rodríguez Carballo E, Bartrons R, Rosa J, Ventura F. MicroRNA-322 (miR-322) and its target protein Tob2 modulate Osterix (Osx) mRNA stability. J Biol Chem. 2013;288:14264-75 pubmed publisher
    ..We demonstrate that overexpression of miR-322 enhances BMP-2 response, increasing the expression of Osx and other osteogenic genes...
  34. Xing W, Cheng S, Wergedal J, Mohan S. Epiphyseal chondrocyte secondary ossification centers require thyroid hormone activation of Indian hedgehog and osterix signaling. J Bone Miner Res. 2014;29:2262-75 pubmed publisher
    ..of thyroid stimulating hormone receptor mutant (Tshr(-/-) ) mice induced expression of Indian hedgehog (Ihh) and Osx in type 2 collagen (Col2)-expressing chondrocytes in the SOC at day 7, which subsequently differentiate into type ..
  35. Okamura H, Yoshida K, Ochiai K, Haneji T. Reduction of protein phosphatase 2A C? enhances bone formation and osteoblast differentiation through the expression of bone-specific transcription factor Osterix. Bone. 2011;49:368-75 pubmed publisher
    ..Our data indicate that PP2A C? plays an important role in the regulation of bone formation and osteoblast differentiation through the bone-related genes. ..
  36. Gu S, Wei N, Yu L, Fei J, Chen Y. Shox2-deficiency leads to dysplasia and ankylosis of the temporomandibular joint in mice. Mech Dev. 2008;125:729-42 pubmed publisher
    ..Shox2 appears to regulate the expression of osteogenic genes and is essential for the development and function of the TMJ. The Shox2 conditional mutant thus provides a unique animal model of TMJ ankylosis. ..
  37. Artigas N, Ureña C, Rodríguez Carballo E, Rosa J, Ventura F. Mitogen-activated protein kinase (MAPK)-regulated interactions between Osterix and Runx2 are critical for the transcriptional osteogenic program. J Biol Chem. 2014;289:27105-17 pubmed publisher
    The transcription factors Runx2 and Osx (Osterix) are required for osteoblast differentiation and bone formation...
  38. Choi H, Ahn Y, Kim T, Bae C, Lee J, You H, et al. TGF-β Signaling Regulates Cementum Formation through Osterix Expression. Sci Rep. 2016;6:26046 pubmed publisher
    ..Interestingly, the expression of osterix (Osx), one of the major regulators of cellular cementum formation, was largely decreased in OCCM-30 cells lacking Tβ..
  39. Elefteriou F, Benson M, Sowa H, Starbuck M, Liu X, Ron D, et al. ATF4 mediation of NF1 functions in osteoblast reveals a nutritional basis for congenital skeletal dysplasiae. Cell Metab. 2006;4:441-51 pubmed
    ..By showing that ATF4-dependent skeletal dysplasiae are treatable by dietary manipulations, this study reveals a molecular connection between nutrition and skeletal development...
  40. Choi Y, Gu Y, Oh J, Lee K. Osterix is regulated by Erk1/2 during osteoblast differentiation. Biochem Biophys Res Commun. 2011;415:472-8 pubmed publisher
    Osterix (Osx) is a novel zinc finger-containing transcription factor that is essential for osteoblast differentiation and bone formation in bone homeostasis...
  41. Lin S, Lee Y, Yu G, Cheng C, Zhou X, Chu K, et al. Endothelial-to-Osteoblast Conversion Generates Osteoblastic Metastasis of Prostate Cancer. Dev Cell. 2017;41:467-480.e3 pubmed publisher
    ..Tumor-induced bone was reduced in trigenic mice (Tie2cre/Osxf/f/SCID) with endothelial-specific deletion of osteoblast cell-fate determinant OSX compared with ..
  42. Ohyama Y, Nifuji A, Maeda Y, Amagasa T, Noda M. Spaciotemporal association and bone morphogenetic protein regulation of sclerostin and osterix expression during embryonic osteogenesis. Endocrinology. 2004;145:4685-92 pubmed
    ..These temporal and spacial expression patterns in vivo and in vitro were in parallel to those of osterix (Osx), which is a critical transcriptional factor for bone formation...
  43. Holm E, Aubin J, Hunter G, Beier F, Goldberg H. Loss of bone sialoprotein leads to impaired endochondral bone development and mineralization. Bone. 2015;71:145-54 pubmed publisher
    ..Expression of the osteogenic markers Alp1, Col1a1, Sp7, Runx2, and Bglap was reduced in the endochondral bone of the neonatal Bsp(-/-) compared to WT tibiae...
  44. Tu Q, Valverde P, Li S, Zhang J, Yang P, Chen J. Osterix overexpression in mesenchymal stem cells stimulates healing of critical-sized defects in murine calvarial bone. Tissue Eng. 2007;13:2431-40 pubmed
    Osterix (Osx) is a zinc-finger-containing transcription factor that is expressed in osteoblasts of all endochondral and membranous bones. In Osx null mice, osteoblast differentiation is impaired, and bone formation is absent...
  45. Khavandgar Z, Poirier C, Clarke C, Li J, WANG N, McKee M, et al. A cell-autonomous requirement for neutral sphingomyelinase 2 in bone mineralization. J Cell Biol. 2011;194:277-89 pubmed publisher
    ..Our data suggest tissue-specific roles for nSMase2 in skeletal tissues. ..
  46. Kaback L, Soung D, Naik A, Geneau G, Schwarz E, Rosier R, et al. Teriparatide (1-34 human PTH) regulation of osterix during fracture repair. J Cell Biochem. 2008;105:219-26 pubmed publisher
    ..Bone marrow cells from 7 days 40 microg/kg of teriparatide treated or saline control mice were cultured and Osx and osteoblast phenotypic gene expression assessed by real-time RT-PCR in these cells...
  47. Liu H, Lin H, Zhang L, Sun Q, Yuan G, Zhang L, et al. miR-145 and miR-143 regulate odontoblast differentiation through targeting Klf4 and Osx genes in a feedback loop. J Biol Chem. 2013;288:9261-71 pubmed publisher
    ..First, KLF4 and OSX bind to their motifs in Dspp and Dmp1 gene promoters and up-regulate their transcription thereby inducing ..
  48. Tian Y, Xu Y, Fu Q, Dong Y. Osterix is required for Sonic hedgehog-induced osteoblastic MC3T3-E1 cell differentiation. Cell Biochem Biophys. 2012;64:169-76 pubmed publisher
    ..Shh) affects osteoblast differentiation in osteoblastic MC3T3-E1 cells and determine the exact role of osterix (Osx) involved in Hh-induced osteoblast differentiation...
  49. Parada C, Han D, Grimaldi A, Sarrión P, Park S, Pelikan R, et al. Disruption of the ERK/MAPK pathway in neural crest cells as a potential cause of Pierre Robin sequence. Development. 2015;142:3734-45 pubmed publisher
    ..Because the ERK pathway serves as a crucial point of convergence for multiple signaling pathways, our study will facilitate a better understanding of the molecular regulatory mechanisms of craniofacial development. ..
  50. He F, Soriano P. Dysregulated PDGFRα signaling alters coronal suture morphogenesis and leads to craniosynostosis through endochondral ossification. Development. 2017;144:4026-4036 pubmed publisher
    ..Our results thus identify a novel mechanism underlying calvarial development in craniosynostosis. ..
  51. Oka K, Oka S, Sasaki T, Ito Y, Bringas P, Nonaka K, et al. The role of TGF-beta signaling in regulating chondrogenesis and osteogenesis during mandibular development. Dev Biol. 2007;303:391-404 pubmed
    ..Collectively, our data suggest that there are differential signal cascades in response to TGF-beta to control chondrogenesis and osteogenesis during mandibular development. ..
  52. Choi Y, Han Y, Lee S, Cheong H, Chun K, Yeo C, et al. Src enhances osteogenic differentiation through phosphorylation of Osterix. Mol Cell Endocrinol. 2015;407:85-97 pubmed publisher
    ..The c-Src interacts with and phosphorylates Osterix. These results suggest that c-Src signaling modulates osteoblast differentiation at least in part through Osterix. ..
  53. Okamura H, Yoshida K, Yang D, Haneji T. Protein phosphatase 2A C? regulates osteoblast differentiation and the expressions of bone sialoprotein and osteocalcin via osterix transcription factor. J Cell Physiol. 2013;228:1031-7 pubmed publisher
  54. Yagi K, Tsuji K, Nifuji A, Shinomiya K, Nakashima K, DeCrombrugghe B, et al. Bone morphogenetic protein-2 enhances osterix gene expression in chondrocytes. J Cell Biochem. 2003;88:1077-83 pubmed
    ..These results indicated that osterix gene is expressed in the primary cultures of chondrocytes and its expression is under the control of BMP-2. ..
  55. González Martín M, Mallo M, Ros M. Long bone development requires a threshold of Hox function. Dev Biol. 2014;392:454-65 pubmed publisher
    ..Our results support Hox genes being required in a dose-dependent manner for long bone cartilage maturation and suggest that and excess of Gli3R mediates a significant part of the Hoxd(Del(11-13)) chondrogenic phenotype. ..
  56. Sakisaka Y, Kanaya S, Nakamura T, Tamura M, Shimauchi H, Nemoto E. p38 MAP kinase is required for Wnt3a-mediated osterix expression independently of Wnt-LRP5/6-GSK3? signaling axis in dental follicle cells. Biochem Biophys Res Commun. 2016;478:527-32 pubmed publisher
    ..Recently, we have reported that Wnt3a increases alkaline phosphatase expression through the induction of osterix (Osx) expression in dental follicle cells, a precursor of cementoblasts...
  57. Hojo H, Ohba S, Yano F, Saito T, Ikeda T, Nakajima K, et al. Gli1 protein participates in Hedgehog-mediated specification of osteoblast lineage during endochondral ossification. J Biol Chem. 2012;287:17860-9 pubmed publisher
    ..On the basis of these findings, we propose that upon Hedgehog input, Gli1 functions collectively with Gli2 and Gli3 in osteogenesis. ..
  58. Kieslinger M, Folberth S, Dobreva G, Dorn T, Croci L, Erben R, et al. EBF2 regulates osteoblast-dependent differentiation of osteoclasts. Dev Cell. 2005;9:757-67 pubmed
    ..Taken together, these data identify EBF2 as a regulator of RANK-RANKL signaling and osteoblast-dependent differentiation of osteoclasts. ..
  59. Zhou Y, Shi H, Li X, Lv P, Li G, Liu Q, et al. Role of endoplasmic reticulum stress in aberrant activation of fluoride-treated osteoblasts. Biol Trace Elem Res. 2013;154:448-56 pubmed publisher
    ..These data indicated that ER stress and UPR were possibly involved in the action of fluoride on osteoblasts. ..
  60. Lu C, Wan Y, Cao J, Zhu X, Yu J, Zhou R, et al. Wnt-mediated reciprocal regulation between cartilage and bone development during endochondral ossification. Bone. 2013;53:566-74 pubmed publisher
  61. Jia J, Tian Q, Ling S, Liu Y, Yang S, Shao Z. miR-145 suppresses osteogenic differentiation by targeting Sp7. FEBS Lett. 2013;587:3027-31 pubmed publisher
    Osteogenesis depends on a coordinated network of transcription factors including Sp7. Emerging evidence indicates that microRNAs (miRNAs) act as pivotal regulators in various biological processes including osteoblast proliferation and ..
  62. Sasagawa S, Takemori H, Uebi T, Ikegami D, Hiramatsu K, Ikegawa S, et al. SIK3 is essential for chondrocyte hypertrophy during skeletal development in mice. Development. 2012;139:1153-63 pubmed publisher
    ..These results demonstrate an essential role for SIK3 in facilitating chondrocyte hypertrophy during skeletogenesis and growth plate maintenance. ..
  63. Tan S, Senarath Yapa K, Chung M, Longaker M, WU J, Nusse R. Wnts produced by Osterix-expressing osteolineage cells regulate their proliferation and differentiation. Proc Natl Acad Sci U S A. 2014;111:E5262-71 pubmed publisher
    ..Therefore, Osterix-expressing cells produce their own Wnts that in turn induce Wnt signaling response, thereby regulating their proliferation and differentiation. ..
  64. Goodnough L, Chang A, Treloar C, Yang J, Scacheri P, ATIT R. Twist1 mediates repression of chondrogenesis by ?-catenin to promote cranial bone progenitor specification. Development. 2012;139:4428-38 pubmed publisher
    ..These findings provide insight into how ?-catenin signaling via Twist1 actively suppresses the formation of cartilage and promotes intramembranous ossification in the skull. ..
  65. Lu X, Ding Y, Niu Q, Xuan S, Yang Y, Jin Y, et al. ClC-3 chloride channel mediates the role of parathyroid hormone [1-34] on osteogenic differentiation of osteoblasts. PLoS ONE. 2017;12:e0176196 pubmed publisher
    ..and we found an obvious decrease in the expression of bone sialoprotein (Ibsp), osteocalcin (Bglap), osterix (Sp7), Alpl and Runx2, the formation of mineralization nodules as well...
  66. Bandyopadhyay A, Tsuji K, Cox K, Harfe B, Rosen V, Tabin C. Genetic analysis of the roles of BMP2, BMP4, and BMP7 in limb patterning and skeletogenesis. PLoS Genet. 2006;2:e216 pubmed
    ..In contrast, we find that the loss of both BMP2 and BMP4 results in a severe impairment of osteogenesis. ..
  67. Chen S, Gluhak Heinrich J, Wang Y, Wu Y, Chuang H, Chen L, et al. Runx2, osx, and dspp in tooth development. J Dent Res. 2009;88:904-9 pubmed publisher
    The transcription factors Runx2 and Osx are necessary for osteoblast and odontoblast differentiation, while Dspp is important for odontoblast differentiation...
  68. Swinehart I, Schlientz A, Quintanilla C, Mortlock D, Wellik D. Hox11 genes are required for regional patterning and integration of muscle, tendon and bone. Development. 2013;140:4574-82 pubmed publisher
    ..Thus, Hox genes are not simply regulators of skeletal morphology as previously thought, but are key factors that regulate regional patterning and integration of the musculoskeletal system. ..
  69. Ortuño M, Susperregui A, Artigas N, Rosa J, Ventura F. Osterix induces Col1a1 gene expression through binding to Sp1 sites in the bone enhancer and proximal promoter regions. Bone. 2013;52:548-56 pubmed publisher
    ..These effects mainly affect the long isoform of Osterix which suggest that the two Osterix isoforms might display some differential effects on the transactivation of bone-specific genes. ..
  70. Chen J, Long F. mTORC1 Signaling Promotes Osteoblast Differentiation from Preosteoblasts. PLoS ONE. 2015;10:e0130627 pubmed publisher
    ..for osteoblast differentiation (Bglap, Ibsp, and Col1a), while slightly increasing early osteoblast markers (Runx2, Sp7, and Alpl)...
  71. Hsiao E, Boudignon B, Halloran B, Nissenson R, Conklin B. Gs G protein-coupled receptor signaling in osteoblasts elicits age-dependent effects on bone formation. J Bone Miner Res. 2010;25:584-93 pubmed publisher
    ..Finally, our model provides a powerful tool for investigating the effects of continuous G(s)-GPCR signaling on dynamic bone growth and remodeling. ..
  72. Mirando A, Maruyama T, Fu J, Yu H, Hsu W. ?-catenin/cyclin D1 mediated development of suture mesenchyme in calvarial morphogenesis. BMC Dev Biol. 2010;10:116 pubmed publisher
    ..These findings advance our knowledge base of Wnt signaling in calvarial morphogenesis, suggesting a key regulatory pathway of Axin2/?-catenin/cyclin D1 in development of the suture mesenchyme. ..
  73. Liu J, Liang C, Guo B, Wu X, Li D, Zhang Z, et al. Increased PLEKHO1 within osteoblasts suppresses Smad-dependent BMP signaling to inhibit bone formation during aging. Aging Cell. 2017;16:360-376 pubmed publisher
  74. Yoshida C, Komori H, Maruyama Z, Miyazaki T, Kawasaki K, Furuichi T, et al. SP7 inhibits osteoblast differentiation at a late stage in mice. PLoS ONE. 2012;7:e32364 pubmed publisher
    RUNX2 and SP7 are essential transcription factors for osteoblast differentiation at an early stage...