Sp1

Summary

Gene Symbol: Sp1
Description: trans-acting transcription factor 1
Alias: 1110003E12Rik, AA450830, AI845540, Sp1-1, transcription factor Sp1
Species: mouse
Products:     Sp1

Top Publications

  1. Yang J, Qin S, Yi C, Ma G, Zhu H, Zhou W, et al. MiR-140 is co-expressed with Wwp2-C transcript and activated by Sox9 to target Sp1 in maintaining the chondrocyte proliferation. FEBS Lett. 2011;585:2992-7 pubmed publisher
    ..Knockdown of miR-140 in limb bud micromass cultures resulted in arrest of chondrogenic proliferation. Sp1, the activator of the cell cycle regulator p15(INK4b), was identified as a target of miR-140 in maintaining the ..
  2. Ma W, Horvath G, Kistler M, Kistler W. Expression patterns of SP1 and SP3 during mouse spermatogenesis: SP1 down-regulation correlates with two successive promoter changes and translationally compromised transcripts. Biol Reprod. 2008;79:289-300 pubmed publisher
    ..in regulation of gene expression, it is important to understand how levels of Krüpple-like transcription factors SP1 and SP3 change in germ cells during spermatogenesis...
  3. Marin M, Karis A, Visser P, Grosveld F, Philipsen S. Transcription factor Sp1 is essential for early embryonic development but dispensable for cell growth and differentiation. Cell. 1997;89:619-28 pubmed
    b>Transcription factor Sp1 has been implicated in the expression of many genes...
  4. Pohlers M, Truss M, Frede U, Scholz A, Strehle M, Kuban R, et al. A role for E2F6 in the restriction of male-germ-cell-specific gene expression. Curr Biol. 2005;15:1051-7 pubmed
    ..Thus, E2F6 is essential for the long-term somatic silencing of certain male-germ-cell-specific genes, but it is dispensable for cell-cycle regulation. ..
  5. Tone Y, Kojima Y, Furuuchi K, Brady M, Yashiro Ohtani Y, Tykocinski M, et al. OX40 gene expression is up-regulated by chromatin remodeling in its promoter region containing Sp1/Sp3, YY1, and NF-kappa B binding sites. J Immunol. 2007;179:1760-7 pubmed
    ..We have shown in this study that basal OX40 promoter activity is regulated by constitutively expressed Sp1/Sp3 and YY1 transcription factors...
  6. Krüger I, Vollmer M, Simmons D, Simmons D, Elsässer H, Philipsen S, et al. Sp1/Sp3 compound heterozygous mice are not viable: impaired erythropoiesis and severe placental defects. Dev Dyn. 2007;236:2235-44 pubmed
    The ubiquitously expressed zinc finger transcription factors Sp1 and Sp3 play critical roles in embryonic development. Sp1 knockout mice die around embryonic day 10.5. Mice lacking Sp3 are postnatal lethal...
  7. Qiu Z, Norflus F, Singh B, Swindell M, Buzescu R, Bejarano M, et al. Sp1 is up-regulated in cellular and transgenic models of Huntington disease, and its reduction is neuroprotective. J Biol Chem. 2006;281:16672-80 pubmed
    ..Our data suggest that enhancement of transcription factor Sp1 contributes to the pathology of HD and demonstrates that its suppression is beneficial.
  8. Bu Y, Gelman I. v-Src-mediated down-regulation of SSeCKS metastasis suppressor gene promoter by the recruitment of HDAC1 into a USF1-Sp1-Sp3 complex. J Biol Chem. 2007;282:26725-39 pubmed
    ..alpha proximal promoter as the minimal v-Src-responsive element, which contains E- and GC-boxes bound by USF1 and Sp1/Sp3, respectively. Both E- and GC-boxes are crucial for v-Src-responsive and basal promoter activities...
  9. Yi T, Tan K, Cho S, Wang Y, Luo J, Zhang W, et al. Regulation of embryonic kidney branching morphogenesis and glomerular development by KISS1 receptor (Gpr54) through NFAT2- and Sp1-mediated Bmp7 expression. J Biol Chem. 2010;285:17811-20 pubmed publisher
    ..Using chromatin immunoprecipitation and luciferase assays, we demonstrate that Gpr54 regulates NFAT2- and Sp1-mediated Bmp7 transcription...
  10. Worrad D, Schultz R. Regulation of gene expression in the preimplantation mouse embryo: temporal and spatial patterns of expression of the transcription factor Sp1. Mol Reprod Dev. 1997;46:268-77 pubmed
    ..We and others have demonstrated that transcription factor Sp1 is present in the mouse oocyte and early cleavage stage preimplantation embryo...

Detail Information

Publications100

  1. Yang J, Qin S, Yi C, Ma G, Zhu H, Zhou W, et al. MiR-140 is co-expressed with Wwp2-C transcript and activated by Sox9 to target Sp1 in maintaining the chondrocyte proliferation. FEBS Lett. 2011;585:2992-7 pubmed publisher
    ..Knockdown of miR-140 in limb bud micromass cultures resulted in arrest of chondrogenic proliferation. Sp1, the activator of the cell cycle regulator p15(INK4b), was identified as a target of miR-140 in maintaining the ..
  2. Ma W, Horvath G, Kistler M, Kistler W. Expression patterns of SP1 and SP3 during mouse spermatogenesis: SP1 down-regulation correlates with two successive promoter changes and translationally compromised transcripts. Biol Reprod. 2008;79:289-300 pubmed publisher
    ..in regulation of gene expression, it is important to understand how levels of Krüpple-like transcription factors SP1 and SP3 change in germ cells during spermatogenesis...
  3. Marin M, Karis A, Visser P, Grosveld F, Philipsen S. Transcription factor Sp1 is essential for early embryonic development but dispensable for cell growth and differentiation. Cell. 1997;89:619-28 pubmed
    b>Transcription factor Sp1 has been implicated in the expression of many genes...
  4. Pohlers M, Truss M, Frede U, Scholz A, Strehle M, Kuban R, et al. A role for E2F6 in the restriction of male-germ-cell-specific gene expression. Curr Biol. 2005;15:1051-7 pubmed
    ..Thus, E2F6 is essential for the long-term somatic silencing of certain male-germ-cell-specific genes, but it is dispensable for cell-cycle regulation. ..
  5. Tone Y, Kojima Y, Furuuchi K, Brady M, Yashiro Ohtani Y, Tykocinski M, et al. OX40 gene expression is up-regulated by chromatin remodeling in its promoter region containing Sp1/Sp3, YY1, and NF-kappa B binding sites. J Immunol. 2007;179:1760-7 pubmed
    ..We have shown in this study that basal OX40 promoter activity is regulated by constitutively expressed Sp1/Sp3 and YY1 transcription factors...
  6. Krüger I, Vollmer M, Simmons D, Simmons D, Elsässer H, Philipsen S, et al. Sp1/Sp3 compound heterozygous mice are not viable: impaired erythropoiesis and severe placental defects. Dev Dyn. 2007;236:2235-44 pubmed
    The ubiquitously expressed zinc finger transcription factors Sp1 and Sp3 play critical roles in embryonic development. Sp1 knockout mice die around embryonic day 10.5. Mice lacking Sp3 are postnatal lethal...
  7. Qiu Z, Norflus F, Singh B, Swindell M, Buzescu R, Bejarano M, et al. Sp1 is up-regulated in cellular and transgenic models of Huntington disease, and its reduction is neuroprotective. J Biol Chem. 2006;281:16672-80 pubmed
    ..Our data suggest that enhancement of transcription factor Sp1 contributes to the pathology of HD and demonstrates that its suppression is beneficial.
  8. Bu Y, Gelman I. v-Src-mediated down-regulation of SSeCKS metastasis suppressor gene promoter by the recruitment of HDAC1 into a USF1-Sp1-Sp3 complex. J Biol Chem. 2007;282:26725-39 pubmed
    ..alpha proximal promoter as the minimal v-Src-responsive element, which contains E- and GC-boxes bound by USF1 and Sp1/Sp3, respectively. Both E- and GC-boxes are crucial for v-Src-responsive and basal promoter activities...
  9. Yi T, Tan K, Cho S, Wang Y, Luo J, Zhang W, et al. Regulation of embryonic kidney branching morphogenesis and glomerular development by KISS1 receptor (Gpr54) through NFAT2- and Sp1-mediated Bmp7 expression. J Biol Chem. 2010;285:17811-20 pubmed publisher
    ..Using chromatin immunoprecipitation and luciferase assays, we demonstrate that Gpr54 regulates NFAT2- and Sp1-mediated Bmp7 transcription...
  10. Worrad D, Schultz R. Regulation of gene expression in the preimplantation mouse embryo: temporal and spatial patterns of expression of the transcription factor Sp1. Mol Reprod Dev. 1997;46:268-77 pubmed
    ..We and others have demonstrated that transcription factor Sp1 is present in the mouse oocyte and early cleavage stage preimplantation embryo...
  11. Doetzlhofer A, Rotheneder H, Lagger G, Koranda M, Kurtev V, Brosch G, et al. Histone deacetylase 1 can repress transcription by binding to Sp1. Mol Cell Biol. 1999;19:5504-11 pubmed
    The members of the Sp1 transcription factor family can act as both negative and positive regulators of gene expression. Here we show that Sp1 can be a target for histone deacetylase 1 (HDAC1)-mediated transcriptional repression...
  12. DeFino M, Wacker J, Lyssand J, Wang E, Hague C. Differential regulation of GPR54 transcription by specificity protein-1 and partial estrogen response element in mouse pituitary cells. Biochem Biophys Res Commun. 2010;393:603-8 pubmed publisher
    ..Supporting experiments show transcription factor SP1 binds directly to the mGPR54 promoter region and activates gene expression...
  13. Rivero S, Ruiz García A, Díaz Guerra M, Laborda J, Garcia Ramirez J. Characterization of a proximal Sp1 response element in the mouse Dlk2 gene promoter. BMC Mol Biol. 2011;12:52 pubmed publisher
    ..Besides, it possesses six GC-boxes, considered as consensus sites for the transcription factor Sp1. Indeed, we report that Sp1 directly binds to the Dlk2 promoter, activates its transcription, and ..
  14. Lomberk G, Urrutia R. The family feud: turning off Sp1 by Sp1-like KLF proteins. Biochem J. 2005;392:1-11 pubmed
    b>Sp1 is one of the best characterized transcriptional activators. The biological importance of Sp1 is underscored by the fact that several hundreds of genes are thought to be regulated by this protein...
  15. Plaisance V, Niederhauser G, Azzouz F, Lenain V, Haefliger J, Waeber G, et al. The repressor element silencing transcription factor (REST)-mediated transcriptional repression requires the inhibition of Sp1. J Biol Chem. 2005;280:401-7 pubmed
    ..Herein, we showed that the transcriptional factor Sp1 was required for expression of most REST target genes both in insulin-secreting cells and neuronal-like cells where ..
  16. Xu J, Rogers M. Modulation of Bone Morphogenetic Protein (BMP) 2 gene expression by Sp1 transcription factors. Gene. 2007;392:221-9 pubmed
    ..BMP2 gene polymorphisms have been linked to osteoporosis and osteoarthritis. Sp1 and related proteins are widely expressed regulators of gene expression whose transcription activating abilities ..
  17. Tang T, Lin X, Yang H, Zhou L, Wang Z, Shan G, et al. Overexpression of antioxidant enzymes upregulates aryl hydrocarbon receptor expression via increased Sp1 DNA-binding activity. Free Radic Biol Med. 2010;49:487-92 pubmed publisher
    ..The objective of this study was to investigate the regulatory role of specificity protein-1 (Sp1) in AhR expression in MAECs that overexpress Cu/Zn-SOD and/or catalase...
  18. Lecuyer E, Herblot S, Saint Denis M, Martin R, Begley C, Porcher C, et al. The SCL complex regulates c-kit expression in hematopoietic cells through functional interaction with Sp1. Blood. 2002;100:2430-40 pubmed
    ..ubiquitous (E2A, LIM- domain binding protein 1 [Ldb-1]) factors, is tethered to DNA via a specificity protein 1 (Sp1) motif, through direct interactions between elements of the SCL complex and the Sp1 zinc finger protein...
  19. Kumar P, Garg R, Bolden G, Pandey K. Interactive roles of Ets-1, Sp1, and acetylated histones in the retinoic acid-dependent activation of guanylyl cyclase/atrial natriuretic peptide receptor-A gene transcription. J Biol Chem. 2010;285:37521-30 pubmed publisher
    ..The present study was conducted to examine the interactive roles of all-trans retinoic acid (ATRA), Ets-1, Sp1, and histone acetylation on the transcriptional regulation and function of the Npr1 gene...
  20. Richardson R, Alekseev O, Alekseev O, O Rand M. Characterization of the NASP promoter in 3T3 fibroblasts and mouse spermatogenic cells. Gene. 2006;371:52-8 pubmed
    ..In the region immediately upstream of the transcription initiation site we identified two closely associated Sp1 binding sites and a binding site for an Ets family member...
  21. Hirasawa N, Torigoe M, Kano K, Ohuchi K. Involvement of Sp1 in lipopolysaccharide-induced expression of HDC mRNA in RAW 264 cells. Biochem Biophys Res Commun. 2006;349:833-7 pubmed
    The involvement of Sp1 in the lipopolysaccharide (LPS)-induced transcription of HDC mRNA in the mouse macrophage-like cell line RAW 264 was analyzed...
  22. Wilmanski J, Siddiqi M, Deitch E, Spolarics Z. Augmented IL-10 production and redox-dependent signaling pathways in glucose-6-phosphate dehydrogenase-deficient mouse peritoneal macrophages. J Leukoc Biol. 2005;78:85-94 pubmed
    ..Alterations in signaling pathways and associated changes in cytokine production may play a role in modulating the inflammatory responses following bacterial or malarial infections in G6PD deficiency. ..
  23. Luk J, Tong M, Mok B, Tam P, Yeung W, Lee K. Sp1 site is crucial for the mouse claudin-19 gene expression in the kidney cells. FEBS Lett. 2004;578:251-6 pubmed
    ..resided in the -39 to -108 region, which contained a number of binding sites for transcription factors including Sp1. Site-directed mutagenesis using specific oligo probes confirmed that Sp1 was crucial for Cldn19 transactivation in ..
  24. de León M, Montañez C, Gomez P, Morales Lázaro S, Tapia Ramírez V, Valadez Graham V, et al. Dystrophin Dp71 expression is down-regulated during myogenesis: role of Sp1 and Sp3 on the Dp71 promoter activity. J Biol Chem. 2005;280:5290-9 pubmed
    ..Electrophoretic mobility shift and chromatin immunoprecipitation assays indicated that Sp1 and Sp3 transcription factors specifically bind to the Sp-binding sites in the minimal Dp71 promoter region...
  25. Walter L, Marynen P, Szpirer J, Levan G, Gunther E. Identification of a novel conserved human gene, TEGT. Genomics. 1995;28:301-4 pubmed
    ..The presence of a nuclear targeting motif indicates that the gene product might localize to the nucleus. The TEGT gene maps to human chromosome 12q12-q13 and belongs to a syntenic group, which is conserved in human, mouse, and rat. ..
  26. Subramanian S, Polikandriotis J, Kelm R, David J, Orosz C, Strauch A. Induction of vascular smooth muscle alpha-actin gene transcription in transforming growth factor beta1-activated myofibroblasts mediated by dynamic interplay between the Pur repressor proteins and Sp1/Smad coactivators. Mol Biol Cell. 2004;15:4532-43 pubmed
    ..The SMA enhancer contains tandem sites for the Sp1 transcriptional activator protein and Puralpha and beta repressor proteins...
  27. Zhu L, Michel V, Bakovic M. Regulation of the mouse CTP: phosphoethanolamine cytidylyltransferase gene Pcyt2 during myogenesis. Gene. 2009;447:51-9 pubmed publisher
    ..Transcription factors Sp1 and Sp3 bind to regions A (-508/-378 bp) and C (-157/-111 bp), and muscle-specific differentiation factor MyoD ..
  28. Li M, Kellems R. Sp1 and Sp3 Are important regulators of AP-2gamma gene transcription. Biol Reprod. 2003;69:1220-30 pubmed
    ..Electrophoretic mobility shift assays demonstrated that Sp1 and Sp3 bind to three sites in the promoter region of the mouse AP-2gamma gene...
  29. Morales Lázaro S, González Ramírez R, Gomez P, Tapia Ramírez V, de León M, Cisneros B. Induction of dystrophin Dp71 expression during neuronal differentiation: opposite roles of Sp1 and AP2alpha in Dp71 promoter activity. J Neurochem. 2010;112:474-85 pubmed publisher
    ..Functional deletion analysis of the Dp71 promoter revealed that a 5'-flanking 159-bp DNA fragment that contains Sp1 and AP2 binding sites is necessary and sufficient for basal expression of this TATA-less promoter, as well as for ..
  30. Rudge T, Johnson L. Synergistic activation of the TATA-less mouse thymidylate synthase promoter by the Ets transcription factor GABP and Sp1. Exp Cell Res. 2002;274:45-55 pubmed
    ..two potential binding sites for members of the Ets family of transcription factors as well as a binding site for Sp1. Promoter mutation analyses revealed that all three of these sites are important for promoter activity...
  31. Zerrouqi A, Pyrzynska B, Febbraio M, Brat D, Van Meir E. P14ARF inhibits human glioblastoma-induced angiogenesis by upregulating the expression of TIMP3. J Clin Invest. 2012;122:1283-95 pubmed publisher
    ..Mechanistically, this regulation occurred at the gene transcription level and was controlled by HDM2-SP1 interplay, where P14ARF relieved a dominant negative interaction of HDM2 with SP1...
  32. Takahara T, Kanazu S, Yanagisawa S, Akanuma H. Heterogeneous Sp1 mRNAs in human HepG2 cells include a product of homotypic trans-splicing. J Biol Chem. 2000;275:38067-72 pubmed
    b>Sp1 is one of the well documented transcription factors, but the whole structure of human Sp1 has not been determined yet...
  33. Ta A, Thakur B, Dutta P, Sinha R, Koley H, Das S. Double-stranded RNA induces cathelicidin expression in the intestinal epithelial cells through phosphatidylinositol 3-kinase-protein kinase Cζ-Sp1 pathway and ameliorates shigellosis in mice. Cell Signal. 2017;35:140-153 pubmed publisher
    ..that poly(I:C) transcriptionally regulates murine cathelicidin-related antimicrobial peptide (mCRAMP) by recruiting Sp1 transcription factor to the GC-box cis-regulatory element at -71bp of the mCRAMP putative promoter...
  34. Saffer J, Thurston S, Annarella M, Compton J. Localization of the gene for the trans-acting transcription factor Sp1 to the distal end of mouse chromosome 15. Genomics. 1990;8:571-4 pubmed
    The mouse chromosomal location for the gene (Sp1-1) encoding the trans-acting transcription factor Sp1 has been determined...
  35. Yang G, Lim C, Li C, Xiao X, Radda G, Li C, et al. FoxO1 inhibits leptin regulation of pro-opiomelanocortin promoter activity by blocking STAT3 interaction with specificity protein 1. J Biol Chem. 2009;284:3719-27 pubmed publisher
    ..that phospho-STAT3 activates POMC promoter in response to leptin signaling through a mechanism that requires an SP1-binding site in the POMC promoter...
  36. Pinacho R, Valdizán E, Pilar Cuéllar F, Prades R, Tarrago T, Haro J, et al. Increased SP4 and SP1 transcription factor expression in the postmortem hippocampus of chronic schizophrenia. J Psychiatr Res. 2014;58:189-96 pubmed publisher
    Altered levels of transcription factor specificity protein 4 (SP4) and 1 (SP1) in the cerebellum, prefrontal cortex and/or lymphocytes have been reported in severe psychiatric disorders, including early psychosis, bipolar disorder, and ..
  37. Kobayashi S, Tanaka T, Moue M, Ohashi S, Nishikawa T. YB-1 gene expression is kept constant during myocyte differentiation through replacement of different transcription factors and then falls gradually under the control of neural activity. Int J Biochem Cell Biol. 2015;68:1-8 pubmed publisher
    ..that in the initial stage of myocyte differentiation, transcription of the YB-1 gene was regulated by E2F1 and Sp1, and was then gradually replaced under the control of both MyoD and myogenin through an E-box sequence in the ..
  38. Chang K, Huang C, Hsu T, Hsu C, Liu J, Chuang C, et al. Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma. Biochem Biophys Res Commun. 2017;493:14-19 pubmed publisher
    ..Here, we investigate whether Sp1, a stress-responsive factor, regulates stemness gene expression and if its inhibition sensitizes cancer cells to ..
  39. Douet V, Heller M, Le Saux O. DNA methylation and Sp1 binding determine the tissue-specific transcriptional activity of the mouse Abcc6 promoter. Biochem Biophys Res Commun. 2007;354:66-71 pubmed
    ..Thus, our data provide the first direct evidence that an epigenetic mechanism regulates the binding of transcription factor Sp1 to the proximal promoter and participates in the tissue-specific expression control of the mouse Abcc6 ..
  40. Ferretti E, Schulz H, Talarico D, Blasi F, Berthelsen J. The PBX-regulating protein PREP1 is present in different PBX-complexed forms in mouse. Mech Dev. 1999;83:53-64 pubmed
  41. Xiao S, Marshak Rothstein A, Ju S. Sp1 is the major fasl gene activator in abnormal CD4(-)CD8(-)B220(+) T cells of lpr and gld mice. Eur J Immunol. 2001;31:3339-48 pubmed
    ..Specific binding sites for Sp1, NFAT, Egr, and NF-kappaB have been identified in the promoter region of the fasl gene...
  42. Wu Y, Matsuo N, Sumiyoshi H, Yoshioka H. The Sp1 and CBF/NF-Y transcription factors cooperatively regulate the mouse pro-alpha3(V) collagen gene (Col5a3) in osteoblastic cells. Acta Med Okayama. 2010;64:95-108 pubmed
    ..An electrophoretic mobility shift assay showed that Sp1/Sp3 and CBF/NF-Y bound to a GC-rich domain (194/186) and a CCAAT box (134/130) in the Col5a3 gene, respectively...
  43. Hadri L, Ozog A, Soncin F, Lompre A. Basal transcription of the mouse sarco(endo)plasmic reticulum Ca2+-ATPase type 3 gene in endothelial cells is controlled by Ets-1 and Sp1. J Biol Chem. 2002;277:36471-8 pubmed
    ..of a minimal promoter region located between bases -97 and +153 that contains one ETS-binding site (EBS) and two Sp1 elements that are essential for basal transcription...
  44. Dhar S, Xu Y, Noel T, St Clair D. Chronic exposure to 12-O-tetradecanoylphorbol-13-acetate represses sod2 induction in vivo: the negative role of p50. Carcinogenesis. 2007;28:2605-13 pubmed
    ..During the activation phase, the levels of p50, p65, specificity protein 1 (Sp1) and nucleophosmin (NPM) increase after TPA treatments...
  45. Zellmer S, Schmidt Heck W, Godoy P, Weng H, Meyer C, Lehmann T, et al. Transcription factors ETF, E2F, and SP-1 are involved in cytokine-independent proliferation of murine hepatocytes. Hepatology. 2010;52:2127-36 pubmed publisher
    ..factor binding sites (TFBS) for ETF (TEA domain family member 2), E2F1 (E2F transcription factor 1), and SP-1 (Sp1 transcription factor) (P < 0.001), all depending on MAPK signaling...
  46. Suttamanatwong S, Jensen E, Schilling J, Franceschi R, Carlson A, Mansky K, et al. Sp proteins and Runx2 mediate regulation of matrix gla protein (MGP) expression by parathyroid hormone. J Cell Biochem. 2009;107:284-92 pubmed publisher
    ..Using gel-mobility shift assays we found that Sp1/Sp3, and Runx2 bind to distinct sites within this region...
  47. Negoro H, Okinami T, Kanematsu A, Imamura M, Tabata Y, Ogawa O. Role of Rev-erb? domains for transactivation of the connexin43 promoter with Sp1. FEBS Lett. 2013;587:98-103 pubmed publisher
    ..However, we recently reported that Rev-erb? activates connexin43 transcription by forming a complex with Sp1. Here we show that heme, a REV-ERB ligand, is dispensable for this novel mechanism and that Rev-erb?, having ..
  48. Gollner H, Bouwman P, Mangold M, Karis A, Braun H, Rohner I, et al. Complex phenotype of mice homozygous for a null mutation in the Sp4 transcription factor gene. Genes Cells. 2001;6:689-97 pubmed
    Sp4 is a zinc finger transcription factor which is closely related to Sp1 and Sp3. All three proteins recognize the same DNA elements and can act as transcriptional activators through glutamine-rich activation domains...
  49. Niger C, Lima F, Yoo D, Gupta R, Buo A, Hebert C, et al. The transcriptional activity of osterix requires the recruitment of Sp1 to the osteocalcin proximal promoter. Bone. 2011;49:683-92 pubmed publisher
    ..While Osx can act at canonical Sp1 DNA-binding sites and/or interact with NFATc1 to cooperatively regulate transcription in some osteoblast promoters, ..
  50. Bondeva T, Wolf G. Advanced glycation end products suppress neuropilin-1 expression in podocytes by a reduction in Sp1-dependent transcriptional activity. Am J Nephrol. 2009;30:336-45 pubmed publisher
    ..Point mutations of proximal and distal Sp1-like sites inhibited basal NRP1 promoter activity...
  51. Banchio C, Schang L, Vance D. Phosphorylation of Sp1 by cyclin-dependent kinase 2 modulates the role of Sp1 in CTP:phosphocholine cytidylyltransferase alpha regulation during the S phase of the cell cycle. J Biol Chem. 2004;279:40220-6 pubmed
    ..E. (2001) J. Biol. Chem. 276, 43688-43692) and that this activation is driven by increased binding of Sp1 to the CTalpha promoter (Banchio, C., Schang, L. M., and Vance, D. E. (2003) J. Biol. Chem. 278, 32457-32464)...
  52. Azahri N, Di Bartolo B, Khachigian L, Kavurma M. Sp1, acetylated histone-3 and p300 regulate TRAIL transcription: mechanisms of PDGF-BB-mediated VSMC proliferation and migration. J Cell Biochem. 2012;113:2597-606 pubmed publisher
    ..was increased by PDGF-BB and subsequently inhibited by dominant-negative Sp1, suggesting that the transcription factor Sp1 plays a role...
  53. Carver B, Plosa E, Stinnett A, Blackwell T, Prince L. Interactions between NF-?B and SP3 connect inflammatory signaling with reduced FGF-10 expression. J Biol Chem. 2013;288:15318-25 pubmed publisher
    ..Here, we show that interactions between the RELA subunit of NF-?B and SP3 suppress SP1-mediated FGF-10 expression...
  54. Dong Z, Wan L, Wang R, Shi Y, Liu G, Zheng S, et al. (-)-Epicatechin Suppresses Angiotensin II-induced Cardiac Hypertrophy via the Activation of the SP1/SIRT1 Signaling Pathway. Cell Physiol Biochem. 2017;41:2004-2015 pubmed publisher
    ..The effects of EPI were accompanied with an up-regulation of SP1 and SIRT1, and were abolished by SP1 inhibition...
  55. Okamoto K, Okamoto Y, Kawakubo T, Iwata J, Yasuda Y, Tsukuba T, et al. Role of the transcription factor Sp1 in regulating the expression of the murine cathepsin E gene. J Biochem. 2012;151:263-72 pubmed publisher
    ..in upstream of exon 1 revealed that this site contained putative binding regions for the transcription factors Sp1, AP-1 and cEts-1 essential for the expression of CE gene...
  56. Kang Q, Chen A. Curcumin inhibits srebp-2 expression in activated hepatic stellate cells in vitro by reducing the activity of specificity protein-1. Endocrinology. 2009;150:5384-94 pubmed publisher
    ..These results provide novel insights into molecular mechanisms by which curcumin inhibits LDL-induced HSC activation. ..
  57. Chung S, Choi H, Cho Y, Lee H, Park K. Sp1 mediates repression of the resistin gene by PPARgamma agonists in 3T3-L1 adipocytes. Biochem Biophys Res Commun. 2006;348:253-8 pubmed
    ..TZD could not repress the expression of the resistin gene in the presence of mithramycin A (an Sp1 binding inhibitor). Sp1 binding site of the resistin promoter (-122/-114bp) was necessary for the repression...
  58. Yao D, Taguchi T, Matsumura T, Pestell R, Edelstein D, Giardino I, et al. High glucose increases angiopoietin-2 transcription in microvascular endothelial cells through methylglyoxal modification of mSin3A. J Biol Chem. 2007;282:31038-45 pubmed
    ..This novel mechanism for regulating gene expression may play a role in the pathobiology of diabetic vascular disease. ..
  59. Sohl M, Lanner F, Farnebo F. Sp1 mediate hypoxia induced ephrinB2 expression via a hypoxia-inducible factor independent mechanism. Biochem Biophys Res Commun. 2010;391:24-7 pubmed publisher
    ..MAZ and Sp1 are known to regulate transcription together and have been shown to bind to the same sites within promoters...
  60. Yajima S, Lee S, Minowa T, Mouradian M. Sp family transcription factors regulate expression of rat D2 dopamine receptor gene. DNA Cell Biol. 1998;17:471-9 pubmed
    ..receptor gene is transcribed from a TATA-less promoter that has an initiator-like sequence and several putative Sp1 binding sites...
  61. Chang A, Garside V, Fournier M, Smrz J, Vrljicak P, Umlandt P, et al. A Notch-dependent transcriptional hierarchy promotes mesenchymal transdifferentiation in the cardiac cushion. Dev Dyn. 2014;243:894-905 pubmed publisher
    ..Functionally, we show these 4 TFs regulate EMT in AVC explant assays. These novel signaling pathways downstream of Notch are potentially relevant to valve development. ..
  62. Botella L, Sanchez Elsner T, Sanz Rodriguez F, Kojima S, Shimada J, Guerrero Esteo M, et al. Transcriptional activation of endoglin and transforming growth factor-beta signaling components by cooperative interaction between Sp1 and KLF6: their potential role in the response to vascular injury. Blood. 2002;100:4001-10 pubmed
    ..a GC-rich tract required for basal activity of the endoglin promoter driven by the related GC box binding protein, Sp1. In cells lacking Sp1 and KLF6, transfected KLF6 and Sp1 cooperatively transactivated the endoglin promoter and ..
  63. Vorovich E, Ratovitski E. Dual regulation of TERT activity through transcription and splicing by DeltaNP63alpha. Aging (Albany NY). 2008;1:58-67 pubmed
    ..through the down regulation of the SIRT1 protein levels, inactivation of p53 deacetylation, decrease of the p53/Sp1 protein-protein interaction, and the overall induction of mTERT transcription regulation...
  64. Moreno Aliaga M, Swarbrick M, Lorente Cebrián S, Stanhope K, Havel P, Martinez J. Sp1-mediated transcription is involved in the induction of leptin by insulin-stimulated glucose metabolism. J Mol Endocrinol. 2007;38:537-46 pubmed
    ..effect of insulin-mediated glucose metabolism on leptin transcription was mediated by a previously identified Sp1 site...
  65. Dreveny I, Kondo H, Uchiyama K, Shaw A, Zhang X, Freemont P. Structural basis of the interaction between the AAA ATPase p97/VCP and its adaptor protein p47. EMBO J. 2004;23:1030-9 pubmed
    ..We also propose a classification for ubiquitin-like domains containing or lacking a longer S3/S4 loop. ..
  66. Hart M, Gorzolla I, Schittenhelm J, Robson S, Eltzschig H. SP1-dependent induction of CD39 facilitates hepatic ischemic preconditioning. J Immunol. 2010;184:4017-24 pubmed publisher
    ..In additional in vivo studies, small interfering RNA treatment was used to achieve repression of the transcription factor Sp1, known to be implicated in CD39 transcriptional regulation...
  67. Heibel S, Lopez G, Panglao M, Sodha S, Mariño Ramírez L, Tuchman M, et al. Transcriptional regulation of N-acetylglutamate synthase. PLoS ONE. 2012;7:e29527 pubmed publisher
    ..DNA-protein pull-down assays and chromatin immunoprecipitation confirmed binding of Sp1 and CREB, but not C/EBP in the promoter and HNF-1 and NF-Y, but not SMAD3 or AP-2 in the enhancer...
  68. Dang X, Raffler N, Ley K. Transcriptional regulation of mouse L-selectin. Biochim Biophys Acta. 2009;1789:146-52 pubmed publisher
    ..Site-directed mutagenesis and chromatin immunoprecipitation showed that Mzf1, Klf2, Sp1, Ets1, and Irf1 bind to and activate the mouse sell promoter...
  69. Bierne H, Tham T, Batsche E, Dumay A, Leguillou M, Kernéis Golsteyn S, et al. Human BAHD1 promotes heterochromatic gene silencing. Proc Natl Acad Sci U S A. 2009;106:13826-31 pubmed publisher
    ..This region contains DNA-binding sequences for the transcription factor SP1, with which BAHD1 coimmunoprecipitates...
  70. Ueno Shuto K, Kato K, Tasaki Y, Sato M, Sato K, Uchida Y, et al. Lipopolysaccharide decreases single immunoglobulin interleukin-1 receptor-related molecule (SIGIRR) expression by suppressing specificity protein 1 (Sp1) via the Toll-like receptor 4 (TLR4)-p38 pathway in monocytes and neutrophils. J Biol Chem. 2014;289:18097-109 pubmed publisher
    ..Finally, reporter gene and chromatin immunoprecipitation assays demonstrated that Sp1 is a key factor that directly binds to the proximal promoter of SIGIRR gene and consequently regulates basal SIGIRR ..
  71. Maeda K, Nishiyama C, Ogawa H, Okumura K. GATA2 and Sp1 positively regulate the c-kit promoter in mast cells. J Immunol. 2010;185:4252-60 pubmed publisher
    ..that a GC-box in the c-kit promoter was critical for transcriptional activity and was bound to the transcription factor Sp1 as assessed using reporter assay and electrophoretic mobility assay...
  72. Andrews G, Lee D, Ravindra R, Lichtlen P, Sirito M, Sawadogo M, et al. The transcription factors MTF-1 and USF1 cooperate to regulate mouse metallothionein-I expression in response to the essential metal zinc in visceral endoderm cells during early development. EMBO J. 2001;20:1114-22 pubmed
    ..Expression of MT-I in visceral endoderm cells was dependent on maternal dietary zinc. Thus, the essential metal, zinc, apparently provides the signaling ligand that activates cell-specific MT-I expression in visceral endoderm cells. ..
  73. Gaur P, Prasad S. Alterations in the Sp1 binding and Fmr-1 gene expression in the cortex of the brain during maturation and aging of mouse. Mol Biol Rep. 2014;41:6855-63 pubmed publisher
    ..Our data suggests that age-dependent alteration in the Fmr-1 gene expression is associated with Sp1 interaction with Fmr-1 promoter which in turn might be related with cognitive development during brain maturation ..
  74. Bhattacharyya S, Feferman L, Tobacman J. Increased expression of colonic Wnt9A through Sp1-mediated transcriptional effects involving arylsulfatase B, chondroitin 4-sulfate, and galectin-3. J Biol Chem. 2014;289:17564-75 pubmed publisher
    ..Nuclear galectin-3 increased and mediated increased binding of Sp1 to the Sp1 consensus sequence in the Wnt9A promoter, shown by oligonucleotide-binding assay and by chromatin ..
  75. Zaniolo K, Desnoyers S, Leclerc S, Guérin S. Regulation of poly(ADP-ribose) polymerase-1 (PARP-1) gene expression through the post-translational modification of Sp1: a nuclear target protein of PARP-1. BMC Mol Biol. 2007;8:96 pubmed
    ..of the PARP-1 gene essentially rely on the opposite regulatory actions of two distinct transcription factors, Sp1 and NFI...
  76. Stevens H, Cham K, Hughes D, Sun R, Sample J, Bubb V, et al. CTCF and Sp1 interact with the Murine gammaherpesvirus 68 internal repeat elements. Virus Genes. 2012;45:265-73 pubmed
    ..This cell line model was used to investigate transcription factor occupancy (CCCTC binding factor [CTCF] and Sp1) of the two internal repeat elements in the viral genome during latency and reactivation using chromatin ..
  77. Lim K, Chang H. O-GlcNAc modification of Sp1 inhibits the functional interaction between Sp1 and Oct1. FEBS Lett. 2009;583:512-20 pubmed publisher
    b>Sp1 is a ubiquitous transcription factor that is modified by multiple O-linked N-acetylglucosamines (O-GlcNAc). Previously, O-GlcNAcylation of a specific site of Sp1 was shown to inhibit Sp1 transcriptional activity...
  78. Speranza F, Mahankali M, Gomez Cambronero J. Macrophage migration arrest due to a winning balance of Rac2/Sp1 repression over ?-catenin-induced PLD expression. J Leukoc Biol. 2013;94:953-62 pubmed publisher
    ..links the Rac2-dependent arrest of chemotaxis to decreased expression of PLD2 through the transcription regulator Sp1. Prolonged Rac2 activity leads to nuclear overactivation of Sp1, which acts as a repressor for PLD2...
  79. Wegener A, Küspert M, Sock E, Philipsen S, Suske G, Wegner M. Sp2 is the only glutamine-rich specificity protein with minor impact on development and differentiation in myelinating glia. J Neurochem. 2017;140:245-256 pubmed publisher
    ..Previous in vitro work had pointed to a role of the zinc finger containing specificity proteins Sp1 and Sp3 as major regulators of glial differentiation and myelination...
  80. Eltzschig H, Kohler D, Eckle T, Kong T, Robson S, Colgan S. Central role of Sp1-regulated CD39 in hypoxia/ischemia protection. Blood. 2009;113:224-32 pubmed publisher
    ..chromatin immunoprecipitation, and inhibition by antisense, revealed a critical role for transcription-factor Sp1 in hypoxia-induction of CD39...
  81. Devi Y, Shehu A, Stocco C, Halperin J, Le J, Seibold A, et al. Regulation of transcription factors and repression of Sp1 by prolactin signaling through the short isoform of its cognate receptor. Endocrinology. 2009;150:3327-35 pubmed publisher
    ..that signaling through PRL-RS does not activate the JaK/Stat in vivo but leads to severe down-regulation of Sp1 expression, DNA binding activity, and nuclear localization, events that appear to involve the calmodulin-dependent ..
  82. Schaffler A, Ehling A, Neumann E, Herfarth H, Paul G, Tarner I, et al. Role of specificity protein-1, PPARgamma, and pituitary protein transcription factor-1 in transcriptional regulation of the murine CORS-26 promoter. Biochim Biophys Acta. 2004;1678:150-6 pubmed
    ..Pit-1 and PPARgamma can bind specifically to the promoter and might play an additive functional role in gene regulation of murine CORS-26. ..
  83. Niu Y, Mo D, Qin L, Wang C, Li A, Zhao X, et al. Lipopolysaccharide-induced miR-1224 negatively regulates tumour necrosis factor-? gene expression by modulating Sp1. Immunology. 2011;133:8-20 pubmed publisher
    ..With public databases of miRNA target prediction, miR-1224 was shown to bind to the 3' untranslated region (UTR) of Sp1 mRNA, whose coding product controls TNF-? expression at the transcriptional level...
  84. Izumi M, Yokoi M, Nishikawa N, Miyazawa H, Sugino A, Yamagishi M, et al. Transcription of the catalytic 180-kDa subunit gene of mouse DNA polymerase alpha is controlled by E2F, an Ets-related transcription factor, and Sp1. Biochim Biophys Acta. 2000;1492:341-52 pubmed
    ..Electrophoretic mobility shift assays showed that Sp1 binds to the GC box...
  85. Xing X, Manske P, Li Y, Lou J. The role of Sp1 in BMP2-up-regulated Erk2 gene expression. Biochem Biophys Res Commun. 2002;297:116-24 pubmed
    ..increases the binding of cell nuclear extracts to the -148/-42 fragment, and the BMP2-enhanced binding bands are Sp1 transcription factors...
  86. Banchio C, Lingrell S, Vance D. Role of histone deacetylase in the expression of CTP:phosphocholine cytidylyltransferase alpha. J Biol Chem. 2006;281:10010-5 pubmed
    ..We have examined the contributions of the Sp1 and E2F binding sites in the repression of CTalpha gene expression...
  87. Park G, Horie T, Kanayama T, Fukasawa K, Iezaki T, Onishi Y, et al. The transcriptional modulator Ifrd1 controls PGC-1? expression under short-term adrenergic stimulation in brown adipocytes. FEBS J. 2017;284:784-795 pubmed publisher
    ..Pgc1a promoter activity enhanced by the transcription factor specificity protein 1 (Sp1) was markedly repressed by co-introduction of Ifrd1 in brown adipocytes, whereas the repression was markedly ..
  88. Chuang J, Kao T, Lin S, Wu A, Lee P, SU T, et al. Specificity protein 1-zinc finger protein 179 pathway is involved in the attenuation of oxidative stress following brain injury. Redox Biol. 2017;11:135-143 pubmed publisher
    ..that oxidative stress increases Znf179 nuclear shuttling and its interaction with specificity protein 1 (Sp1)...
  89. Peñuelas Urquides K, Becerril Esquivel C, Mendoza de León L, Silva Ramirez B, Davila Velderrain J, Cisneros B, et al. Transcription factors YY1, Sp1 and Sp3 modulate dystrophin Dp71 gene expression in hepatic cells. Biochem J. 2016;473:1967-76 pubmed publisher
    ..Using EMSAs and ChIP, we showed that the Sp1 (specificity protein 1), Sp3 (specificity protein 3) and YY1 (Yin and Yang 1) transcription factors bind to the ..
  90. Ito T, Williams Nate Y, Iwai M, Tsuboi Y, Hagiyama M, Ito A, et al. Transcriptional regulation of the CADM1 gene by retinoic acid during the neural differentiation of murine embryonal carcinoma P19 cells. Genes Cells. 2011;16:791-802 pubmed publisher
    ..This element contains a putative binding site for transcription factor Sp1, named Sp1-binding site-1 (Sp1BS-1)...
  91. Miura A, Kambe Y, Inoue K, Tatsukawa H, Kurihara T, Griffin M, et al. Pituitary adenylate cyclase-activating polypeptide type 1 receptor (PAC1) gene is suppressed by transglutaminase 2 activation. J Biol Chem. 2013;288:32720-30 pubmed publisher
    ..a previous work, we demonstrated that nerve growth factor augmented PAC1 gene expression through the activation of Sp1 via the Ras/MAPK pathway...
  92. González Ramírez R, Martínez Hernández E, Sandoval A, Felix R. Transcription factor Sp1 regulates T-type Ca(2+) channel CaV 3.1 gene expression. J Cell Physiol. 2014;229:551-60 pubmed publisher
    ..minimal promoter that includes two potential transcription start sites and four binding sites for the transcription factor Sp1. The ability of one of these sites to interact with the transcription factor was confirmed by ..
  93. Prasanna Kumar S, Thippeswamy G, Sheela M, Prabhakar B, Salimath B. Butyrate-induced phosphatase regulates VEGF and angiogenesis via Sp1. Arch Biochem Biophys. 2008;478:85-95 pubmed publisher
    b>Sp1 is a ubiquitous transcription factor and master regulator of various eukaryotic gene expression...
  94. Liu M, Leibowitz J, Clark D, Mendicino M, Ning Q, Ding J, et al. Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3. Eur J Biochem. 2003;270:2274-86 pubmed
    ..cells revealed that the nucleoprotein complexes that form on this positive regulatory domain (PRD) contain Sp1/Sp3 family members, Oct-1, and Ets-1...
  95. Isono K, Mizutani Koseki Y, Komori T, Schmidt Zachmann M, Koseki H. Mammalian polycomb-mediated repression of Hox genes requires the essential spliceosomal protein Sf3b1. Genes Dev. 2005;19:536-41 pubmed
    ..Importantly, the levels of Sf3b1 in PcG complexes were decreased in Sf3b1-heterozygous embryos. These findings suggest that Sf3b1-PcG protein interaction is essential for true PcG-mediated repression of Hox genes. ..
  96. Necela B, Su W, Thompson E. Peroxisome proliferator-activated receptor gamma down-regulates follistatin in intestinal epithelial cells through SP1. J Biol Chem. 2008;283:29784-94 pubmed publisher
    ..Analysis of the follistatin promoter revealed consensus binding sites for AP-1, AP-2, and Sp1. Targeting the AP-1 pathway with SP600125, an inhibitor of JNK, and TAM67, a dominant negative c-Jun, had no effect ..
  97. Luzina I, Tsymbalyuk N, Choi J, Hasday J, Atamas S. CCL18-stimulated upregulation of collagen production in lung fibroblasts requires Sp1 signaling and basal Smad3 activity. J Cell Physiol. 2006;206:221-8 pubmed
    ..In this study, involvement of Sp1 and Smad3 in CCL18 signaling in primary human pulmonary fibroblast cultures was investigated...