Slc9a3r1

Summary

Gene Symbol: Slc9a3r1
Description: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
Alias: EBP-50, NHE-RF, NHERF-1, NHERF1, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, EBP50, ezrin-radixin-moesin-binding phosphoprotein 50, regulatory cofactor of Na(+)/H(+) exchanger, sodium-hydrogen exchanger regulatory factor 1, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1, solute carrier family 9 isoform A3 regulatory factor 1
Species: mouse
Products:     Slc9a3r1

Top Publications

  1. Hall R, Premont R, Chow C, Blitzer J, Pitcher J, Claing A, et al. The beta2-adrenergic receptor interacts with the Na+/H+-exchanger regulatory factor to control Na+/H+ exchange. Nature. 1998;392:626-30 pubmed
    ..Our findings indicate that agonist-dependent beta2-adrenergic receptor binding of NHERF plays a role in beta2-adrenergic receptor-mediated regulation of Na+/H+ exchange. ..
  2. Kreimann E, Morales F, de Orbeta Cruz J, Takahashi Y, Adams H, Liu T, et al. Cortical stabilization of beta-catenin contributes to NHERF1/EBP50 tumor suppressor function. Oncogene. 2007;26:5290-9 pubmed
    ..By using gene-deficient mouse embryonic fibroblasts (MEFs), we showed for the first time that NHERF1/EBP50 (Na/H exchanger regulator factor 1/ezrin-radixin-moesin binding phosphoprotein 50), an adapter protein with ..
  3. Shenolikar S, Voltz J, Minkoff C, Wade J, Weinman E. Targeted disruption of the mouse NHERF-1 gene promotes internalization of proximal tubule sodium-phosphate cotransporter type IIa and renal phosphate wasting. Proc Natl Acad Sci U S A. 2002;99:11470-5 pubmed
    ..Phosphate wasting seen in the NHERF-1(-/-) null mice provided a new experimental system for defining the role of PDZ adapters in the hormonal control of ion transport and renal disease. ..
  4. Guerra L, Fanelli T, Favia M, Riccardi S, Busco G, Cardone R, et al. Na+/H+ exchanger regulatory factor isoform 1 overexpression modulates cystic fibrosis transmembrane conductance regulator (CFTR) expression and activity in human airway 16HBE14o- cells and rescues DeltaF508 CFTR functional expression in cystic fibros. J Biol Chem. 2005;280:40925-33 pubmed
    ..The Na(+)/H(+) exchanger regulatory factor isoform 1 (NHERF1) was the first identified CFTR-binding protein...
  5. Itoh K, Sakakibara M, Yamasaki S, Takeuchi A, Arase H, Miyazaki M, et al. Cutting edge: negative regulation of immune synapse formation by anchoring lipid raft to cytoskeleton through Cbp-EBP50-ERM assembly. J Immunol. 2002;168:541-4 pubmed
    ..Cbp/PAG is a Csk-associated membrane adapter protein exclusively localized in a lipid raft. We identified NHERF/EBP50 as a Cbp-binding molecule, which connects the membrane raft and cytoskeleton by binding to both Cbp through its ..
  6. He J, Lau A, Yaffe M, Hall R. Phosphorylation and cell cycle-dependent regulation of Na+/H+ exchanger regulatory factor-1 by Cdc2 kinase. J Biol Chem. 2001;276:41559-65 pubmed
    ..These data reveal that cellular NHERF-1 is phosphorylated during mitosis phase by Cdc2 at Ser(279) and Ser(301) and that this phosphorylation regulates NHERF-1 oligomerization and association with Pin1. ..
  7. Pan Y, Weinman E, Dai J. Na+/H+ exchanger regulatory factor 1 inhibits platelet-derived growth factor signaling in breast cancer cells. Breast Cancer Res. 2008;10:R5 pubmed publisher
    The gene encoding Na+/H+ exchanger regulatory factor 1 (NHERF1) is a putative tumor suppressor gene that harbors frequent loss of heterozygosity (LOH) and intragenic mutations in breast carcinoma...
  8. Takahashi Y, Morales F, Kreimann E, Georgescu M. PTEN tumor suppressor associates with NHERF proteins to attenuate PDGF receptor signaling. EMBO J. 2006;25:910-20 pubmed
    ..We show here that PTEN interacts directly with the NHERF1 and NHERF2 (Na+/H+ exchanger regulatory factor) homologous adaptor proteins through the PDZ motif of PTEN and the ..
  9. Morales F, Takahashi Y, Kreimann E, Georgescu M. Ezrin-radixin-moesin (ERM)-binding phosphoprotein 50 organizes ERM proteins at the apical membrane of polarized epithelia. Proc Natl Acad Sci U S A. 2004;101:17705-10 pubmed
    ..By generating mice deficient for ERM-binding phosphoprotein 50/Na(+)/H(+) exchanger regulatory factor 1 (EBP50/NHERF1), which binds the N-terminal domain of ERM proteins, we found that EBP50 is required for the maintenance of active ..

More Information

Publications75

  1. Chavez J, Hernández González E, Wertheimer E, Visconti P, Darszon A, Trevino C. Participation of the Cl-/HCO(3)- exchangers SLC26A3 and SLC26A6, the Cl- channel CFTR, and the regulatory factor SLC9A3R1 in mouse sperm capacitation. Biol Reprod. 2012;86:1-14 pubmed publisher
    ..is known to be mediated and probably regulated by the Na(+)/H(+) regulatory factor-1 (official symbol, SLC9A3R1)...
  2. Leslie K, Song G, Barrick S, Wehbi V, Vilardaga J, Bauer P, et al. Ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) and nuclear factor-?B (NF-?B): a feed-forward loop for systemic and vascular inflammation. J Biol Chem. 2013;288:36426-36 pubmed publisher
    ..Here we report that the scaffolding protein, ezrin-binding phosphoprotein 50 (EBP50), is a central regulator of macrophage and vascular smooth muscle cells (VSMC) function...
  3. Sultan A, Luo M, Yu Q, Riederer B, Xia W, Chen M, et al. Differential association of the Na+/H+ Exchanger Regulatory Factor (NHERF) family of adaptor proteins with the raft- and the non-raft brush border membrane fractions of NHE3. Cell Physiol Biochem. 2013;32:1386-402 pubmed
    ..Thus, we studied the association of NHE3, NHERF1 (EBP50), NHERF2 (E3KARP), and NHERF3 (PDZK1) with lipid rafts in murine small intestinal BBM...
  4. Cunningham R, Steplock D, Wang F, Huang H, E X, Shenolikar S, et al. Defective parathyroid hormone regulation of NHE3 activity and phosphate adaptation in cultured NHERF-1-/- renal proximal tubule cells. J Biol Chem. 2004;279:37815-21 pubmed
    ..NHERF-1(-/-) cells also do not adapt to alterations in the phosphate content of the media indicating that the defect resides within the cells of the proximal tubule and is not dependent on systemic factors. ..
  5. Lazar C, Cresson C, Lauffenburger D, Gill G. The Na+/H+ exchanger regulatory factor stabilizes epidermal growth factor receptors at the cell surface. Mol Biol Cell. 2004;15:5470-80 pubmed
    ..Although the mechanisms differ, for both RTKs and G protein-coupled receptors, the overall effect of NHERF is to enhance the fraction of receptors present at the cell surface. ..
  6. He P, Zhao L, No Y, Karvar S, Yun C. The NHERF1 PDZ1 domain and IRBIT interact and mediate the activation of Na+/H+ exchanger 3 by ANG II. Am J Physiol Renal Physiol. 2016;311:F343-51 pubmed publisher
    ..We found that NHERF1 was required for ANG II-mediated forward trafficking and activation of NHE3 in cultured cells...
  7. Park M, Ko S, Choi J, Muallem G, Thomas P, Pushkin A, et al. The cystic fibrosis transmembrane conductance regulator interacts with and regulates the activity of the HCO3- salvage transporter human Na+-HCO3- cotransport isoform 3. J Biol Chem. 2002;277:50503-9 pubmed
    ..NBC3 or CFTR from transfected HEK293 cells and from the pancreas and submandibular gland also coimmunoprecipitated EBP50. Glutathione S-transferase-EBP50 pulled down CFTR and hNBC3 from cell lysates when expressed individually and as a ..
  8. Seidler U, Singh A, Chen M, Cinar A, Bachmann O, Zheng W, et al. Knockout mouse models for intestinal electrolyte transporters and regulatory PDZ adaptors: new insights into cystic fibrosis, secretory diarrhoea and fructose-induced hypertension. Exp Physiol. 2009;94:175-9 pubmed publisher
    ..mice for three members of the sodium-hydrogen regulatory factor (NHERF) family of PDZ adaptor proteins, namely NHERF1 (EBP50), NHERF2 (E3KARP) and NHERF3 (PDZK1), has helped to explain why NHERF1 is essential for both normal and ..
  9. Tan P, Furness S, Merkens H, Lin S, McCoy M, Roskelley C, et al. Na+/H+ exchanger regulatory factor-1 is a hematopoietic ligand for a subset of the CD34 family of stem cell surface proteins. Stem Cells. 2006;24:1150-61 pubmed
    ..The results suggest that this cytosolic adaptor protein may be important for mobilization of CD34-type proteins in the plasma membrane and may thereby regulate their ability to block or enhance hematopoietic cell adhesion. ..
  10. Lederer E, Khundmiri S, Weinman E. Role of NHERF-1 in regulation of the activity of Na-K ATPase and sodium-phosphate co-transport in epithelial cells. J Am Soc Nephrol. 2003;14:1711-9 pubmed
    ..These experiments also suggest that the mechanism for retrieval of NaPi IIa transporters from the apical membrane in response to cAMP does not require NHERF. ..
  11. Henkel B, Bintig W, Bhat S, Spehr M, Neuhaus E. NHERF1 in Microvilli of Vomeronasal Sensory Neurons. Chem Senses. 2017;42:25-35 pubmed
    ..We identified the PDZ domain-containing protein, Na+/H+ exchanger regulatory factor-1 (NHERF1), as putative molecular organizer of signal transduction in vomeronasal neurons...
  12. Stokka A, Mosenden R, Ruppelt A, Lygren B, Tasken K. The adaptor protein EBP50 is important for localization of the protein kinase A-Ezrin complex in T-cells and the immunomodulating effect of cAMP. Biochem J. 2009;425:381-8 pubmed publisher
    ..and, together with PAG (phosphoprotein associated with glycosphingolipid-enriched membrane microdomains) and EBP50 [ERM (Ezrin/Radixin/Moesin)-binding phosphoprotein 50], forms a scaffold that positions PKA close to its substrate,..
  13. Giral H, Cranston D, Lanzano L, Caldas Y, Sutherland E, Rachelson J, et al. NHE3 regulatory factor 1 (NHERF1) modulates intestinal sodium-dependent phosphate transporter (NaPi-2b) expression in apical microvilli. J Biol Chem. 2012;287:35047-56 pubmed publisher
    ..Immunofluorescence imaging shows NaPi-2b and two PDZ domain containing proteins, NHERF1 and PDZK1, are expressed in the apical microvillar domain of rat small intestine enterocytes...
  14. Georgescu M, Gagea M, Cote G. NHERF1/EBP50 Suppresses Wnt-?-Catenin Pathway-Driven Intestinal Neoplasia. Neoplasia. 2016;18:512-23 pubmed publisher
    b>NHERF1/EBP50, an adaptor molecule that interacts with ?-catenin, YAP, and PTEN, has been recently implicated in the progression of various human malignancies, including colorectal cancer...
  15. Cunningham R, Esmaili A, Brown E, Biswas R, Murtazina R, Donowitz M, et al. Urine electrolyte, mineral, and protein excretion in NHERF-2 and NHERF-1 null mice. Am J Physiol Renal Physiol. 2008;294:F1001-7 pubmed publisher
  16. Weinman E, Mohanlal V, Stoycheff N, Wang F, Steplock D, Shenolikar S, et al. Longitudinal study of urinary excretion of phosphate, calcium, and uric acid in mutant NHERF-1 null mice. Am J Physiol Renal Physiol. 2006;290:F838-43 pubmed
    ..These studies indicate defects in the renal tubule transport of phosphate, calcium, and uric acid in NHERF-1(-/-) male and female mice that could account for the increased deposition of calcium in the papilla of null mice. ..
  17. Broere N, Chen M, Cinar A, Singh A, Hillesheim J, Riederer B, et al. Defective jejunal and colonic salt absorption and alteredNa(+)/H (+) exchanger 3 (NHE3) activity in NHE regulatory factor 1 (NHERF1) adaptor protein-deficient mice. Pflugers Arch. 2009;457:1079-91 pubmed publisher
    We investigated the role of the Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) on intestinal salt and water absorption, brush border membrane (BBM) morphology, and on the NHE3 mRNA expression, protein abundance, and transport activity ..
  18. Wang X, Willen R, Svensson M, Ljungh A, Wadstrom T. Two-year follow-up of Helicobacter pylori infection in C57BL/6 and Balb/cA mice. APMIS. 2003;111:514-22 pubmed
    ..A reproducible animal model for H. pylori-induced lymphoma and possibly hepatocellular carcinoma is described. Strain diversity may lead to different outcomes of H. pylori infection. ..
  19. Al Ghouleh I, Meijles D, Mutchler S, Zhang Q, Sahoo S, Gorelova A, et al. Binding of EBP50 to Nox organizing subunit p47phox is pivotal to cellular reactive species generation and altered vascular phenotype. Proc Natl Acad Sci U S A. 2016;113:E5308-17 pubmed publisher
    ..In this manuscript, we identify critical requirement of ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50; aka NHERF1) for Nox1 activation and downstream responses...
  20. Tandon C, De Lisle R, Boulatnikov I, Naik P. Interaction of carboxyl-terminal peptides of cytosolic-tail of apactin with PDZ domains of NHERF/EBP50 and PDZK-1/CAP70. Mol Cell Biochem. 2007;302:157-67 pubmed
    ..structures in polarized epithelial cells by connecting filamentous (F)-actin to plasma membrane proteins through EBP50. Previous work showed that the membrane phosphoprotein apactin (an 80-kDa type I membrane protein derived from pro-..
  21. Weinman E, Steplock D, Shenolikar S, Biswas R. Fibroblast growth factor-23-mediated inhibition of renal phosphate transport in mice requires sodium-hydrogen exchanger regulatory factor-1 (NHERF-1) and synergizes with parathyroid hormone. J Biol Chem. 2011;286:37216-21 pubmed publisher
    ..In addition, FGF-23 synergizes with PTH to inhibit phosphate transport by facilitating the activation of the PTH signal transduction pathway. ..
  22. Yang Z, Zimmerman S, Tsunezumi J, Braitsch C, Trent C, Bryant D, et al. Role of CD34 family members in lumen formation in the developing kidney. Dev Biol. 2016;418:66-74 pubmed publisher
    ..Despite this, Endoglycan/Podocalyxin is required for apical recruitment of the adaptor protein NHERF1, but not Ezrin, in podocyte precursors, a subset of the epithelia...
  23. Li M, Wang W, Soroka C, Mennone A, Harry K, Weinman E, et al. NHERF-1 binds to Mrp2 and regulates hepatic Mrp2 expression and function. J Biol Chem. 2010;285:19299-307 pubmed publisher
    ..These findings strongly suggest that NHERF-1 binds to Mrp2, and plays a critical role in the canalicular expression of Mrp2 and its function as a determinant of glutathione-dependent, bile acid-independent bile flow. ..
  24. Evan A, Weinman E, Wu X, Lingeman J, Worcester E, Coe F. Comparison of the pathology of interstitial plaque in human ICSF stone patients to NHERF-1 and THP-null mice. Urol Res. 2010;38:439-52 pubmed publisher
    ..This may be due to the different morphology of the human versus mouse papillary region. Both mouse models appear to be important to characterize further in order to determine how well they mimic human kidney stone disease. ..
  25. Donowitz M, Singh S, Singh P, Salahuddin F, Chen Y, Chakraborty M, et al. Alterations in the proteome of the NHERF1 knockout mouse jejunal brush border membrane vesicles. Physiol Genomics. 2010;42A:200-10 pubmed publisher
    Na/H exchanger regulatory factor 1 (NHERF1) is a scaffold protein made up of two PDZ domains and an ERM binding domain...
  26. Favia M, Guerra L, Fanelli T, Cardone R, Monterisi S, Di Sole F, et al. Na+/H+ exchanger regulatory factor 1 overexpression-dependent increase of cytoskeleton organization is fundamental in the rescue of F508del cystic fibrosis transmembrane conductance regulator in human airway CFBE41o- cells. Mol Biol Cell. 2010;21:73-86 pubmed publisher
    We have demonstrated that Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) overexpression in CFBE41o- cells induces a significant redistribution of F508del cystic fibrosis transmembrane conductance regulator (CFTR) from the cytoplasm to ..
  27. Gomperts B, Gong Cooper X, Hackett B. Foxj1 regulates basal body anchoring to the cytoskeleton of ciliated pulmonary epithelial cells. J Cell Sci. 2004;117:1329-37 pubmed
    ..In the absence of Foxj1, decreased calpastatin expression with decreased ezrin and EBP-50 results in an inability of basal bodies to anchor to the apical cytoskeleton and subsequent failure of axonemal formation. ..
  28. Jin W, Dai J, Zhou H, Xia Q, Zou H, Zeng R. Phosphoproteome analysis of mouse liver using immobilized metal affinity purification and linear ion trap mass spectrometry. Rapid Commun Mass Spectrom. 2004;18:2169-76 pubmed
    ..The use of the linear ion trap considerably enabled exploitation of nanoflow-HPLC/MS/MS, and in addition MS/MS/MS has great potential in phosphoproteome research of relatively complex samples. ..
  29. Treat A, Wheeler D, Stolz D, Tsang M, Friedman P, Romero G. The PDZ Protein Na+/H+ Exchanger Regulatory Factor-1 (NHERF1) Regulates Planar Cell Polarity and Motile Cilia Organization. PLoS ONE. 2016;11:e0153144 pubmed publisher
    ..Here we report that mice lacking the Na+/H+ Exchanger Regulatory Factor 1 (NHERF1/Slc9a3r1, also known as EBP50) develop profound communicating hydrocephalus associated with fewer and disorganized ..
  30. Li X, Xu W, Yin T, Zhao Q, Peng L, Yang J. Temporal and spatial regulation of ezrin-radixin-moesin-binding phosphoprotein-50-kDa (EBP50) during embryo implantation in mouse uterus. Int J Mol Sci. 2012;13:16418-29 pubmed publisher
    ..To date, the mechanism of embryo implantation remains unclear. Ezrin-radixin-moesin-binding protein-50-kDa (EBP50) is a scaffold protein, which has been shown to play an important role in cancer development...
  31. Loughran P, Stolz D, Barrick S, Wheeler D, Friedman P, Rachubinski R, et al. PEX7 and EBP50 target iNOS to the peroxisome in hepatocytes. Nitric Oxide. 2013;31:9-19 pubmed publisher
    ..Proteomic studies using MALDI-MS identified iNOS association with the 50-kD ezrin binding PDZ protein (EBP50)...
  32. Gisler S, Pribanic S, Bacic D, Forrer P, Gantenbein A, Sabourin L, et al. PDZK1: I. a major scaffolder in brush borders of proximal tubular cells. Kidney Int. 2003;64:1733-45 pubmed
    ..We hypothesize that PDZK1 and NHERF-1 establish an extended network beneath the apical membrane to which membrane proteins and regulatory components are anchored. ..
  33. Weinman E, Steplock D, Shenolikar S. NHERF-1 uniquely transduces the cAMP signals that inhibit sodium-hydrogen exchange in mouse renal apical membranes. FEBS Lett. 2003;536:141-4 pubmed
    ..As the protein levels for NHE3, NHERF-2, PKA and ezrin were not changed in the NHERF-1 (-/-) BBMs, the data suggest a unique role for NHERF-1 in cAMP-mediated inhibition of NHE3 activity in the renal proximal tubule of the mouse. ..
  34. Voltz J, Brush M, Sikes S, Steplock D, Weinman E, Shenolikar S. Phosphorylation of PDZ1 domain attenuates NHERF-1 binding to cellular targets. J Biol Chem. 2007;282:33879-87 pubmed
    NHERF-1 (Na(+)-H(+) exchanger regulatory factor 1, also known as EBP50 ezrin-binding protein of 50 kDa) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain...
  35. Weinman E, Biswas R, Peng G, Peng Q, Shen L, Turner C, et al. Parathyroid hormone inhibits renal phosphate transport by phosphorylation of serine 77 of sodium-hydrogen exchanger regulatory factor-1. J Clin Invest. 2007;117:3412-20 pubmed
    ..These results indicate that PTH-mediated inhibition of renal phosphate transport involves phosphorylation of S77 of the NHERF-1 PDZ I domain and the dissociation of NHERF-1/Npt2a complexes. ..
  36. Giral H, Lanzano L, Caldas Y, Blaine J, Verlander J, LEI T, et al. Role of PDZK1 protein in apical membrane expression of renal sodium-coupled phosphate transporters. J Biol Chem. 2011;286:15032-42 pubmed publisher
    ..In this regard, direct interaction between NaPi-2c and PDZK1 seems to play an important role in the physiological regulation of NaPi-2c. ..
  37. Kamiya K, Michel V, Giraudet F, Riederer B, Foucher I, Papal S, et al. An unusually powerful mode of low-frequency sound interference due to defective hair bundles of the auditory outer hair cells. Proc Natl Acad Sci U S A. 2014;111:9307-12 pubmed publisher
    ..Here, we identified the submembrane scaffold protein Nherf1 as a hair-bundle component of the differentiating outer hair cells (OHCs)...
  38. Capuano P, Bacic D, Roos M, Gisler S, Stange G, Biber J, et al. Defective coupling of apical PTH receptors to phospholipase C prevents internalization of the Na+-phosphate cotransporter NaPi-IIa in Nherf1-deficient mice. Am J Physiol Cell Physiol. 2007;292:C927-34 pubmed
    ..three COOH-terminal amino acids with the PDZ protein Na(+)/H(+)-exchanger isoform 3 regulatory factor (NHERF)-1 (NHERF1)...
  39. Xue J, Douglas R, Zhou D, Lim J, Boron W, Haddad G. Expression of Na+/H+ and HCO3- -dependent transporters in Na+/H+ exchanger isoform 1 null mutant mouse brain. Neuroscience. 2003;122:37-46 pubmed
    ..The alteration is region-specific. An increase in acid extruders (e.g. NHE3) and a decrease in acid loaders (e.g. AE3) suggest that there are some compensatory mechanisms that occur in NHE1 null mutant mice. ..
  40. Li M, Soroka C, Harry K, Boyer J. CFTR-associated ligand is a negative regulator of Mrp2 expression. Am J Physiol Cell Physiol. 2017;312:C40-C46 pubmed publisher
    ..Our findings suggest that CAL interacts with Mrp2 and is a negative regulator of Mrp2 expression. ..
  41. Murtazina R, Kovbasnjuk O, Zachos N, Li X, Chen Y, Hubbard A, et al. Tissue-specific regulation of sodium/proton exchanger isoform 3 activity in Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) null mice. cAMP inhibition is differentially dependent on NHERF1 and exchange protein directly activated by cAMP in ileum ve. J Biol Chem. 2007;282:25141-51 pubmed
    The multi-PDZ domain containing protein Na(+)/H(+) Exchanger Regulatory Factor 1 (NHERF1) binds to Na(+)/H(+) exchanger 3 (NHE3) and is associated with the brush border (BB) membrane of murine kidney and small intestine...
  42. Wang B, Yang Y, Liu L, Blair H, Friedman P. NHERF1 regulation of PTH-dependent bimodal Pi transport in osteoblasts. Bone. 2013;52:268-77 pubmed publisher
    ..the activity of the type 2 sodium-phosphate cotransporter (Npt2), and this action requires the PDZ protein NHERF1. Osteoblasts express Npt2 and in response to PTH enhance osteogenesis by increasing mineralized matrix...
  43. Lee Y, Ouyang Y, Giffard R. Regulation of the rat brain Na+ -driven Cl-/HCO3 - exchanger involves protein kinase A and a multiprotein signaling complex. FEBS Lett. 2006;580:4865-71 pubmed
    ..Disruption of actin filaments also decreased rb2NCBE activity. EBP50 and FLAG-rb2NCBE were reciprocally co-immunoprecipitated from rb2NCBE transfected cells...
  44. Liu L, Alonso V, Guo L, Tourkova I, Henderson S, Almarza A, et al. Na+/H+ exchanger regulatory factor 1 (NHERF1) directly regulates osteogenesis. J Biol Chem. 2012;287:43312-21 pubmed publisher
    ..The absence of the PDZ domain protein Na(+)/H(+) exchange regulatory factor 1 (NHERF1) in mice, or its mutation in humans, causes osteomalacia believed to reflect renal phosphate wasting...
  45. Francis S, Sfakianos J, Lo B, Mellman I. A hierarchy of signals regulates entry of membrane proteins into the ciliary membrane domain in epithelial cells. J Cell Biol. 2011;193:219-33 pubmed publisher
    ..Podocalyxin is immobilized by its PDZ interaction motif binding to NHERF1 and thereby to the apical actin network via ERM family members...
  46. Sadakata H, Okazawa H, Sato T, Supriatna Y, Ohnishi H, Kusakari S, et al. SAP-1 is a microvillus-specific protein tyrosine phosphatase that modulates intestinal tumorigenesis. Genes Cells. 2009;14:295-308 pubmed publisher
    ..These results thus suggest that SAP-1 is a microvillus-specific RPTP that regulates intestinal tumorigenesis. ..
  47. Luo J, Chen H, Kintner D, Shull G, Sun D. Decreased neuronal death in Na+/H+ exchanger isoform 1-null mice after in vitro and in vivo ischemia. J Neurosci. 2005;25:11256-68 pubmed
    ..05). These results imply that NHE1 activity disrupts Na+ and Ca2+ homeostasis and contributes to ischemic neuronal damage. ..
  48. Weinman E, Wang Y, Wang F, Greer C, Steplock D, Shenolikar S. A C-terminal PDZ motif in NHE3 binds NHERF-1 and enhances cAMP inhibition of sodium-hydrogen exchange. Biochemistry. 2003;42:12662-8 pubmed
    ..Together, the data suggested that the PDZ interaction between the NHE3 C-terminus and a NHERF-1 PDZ domain enhanced the regulation of sodium-hydrogen exchange by cAMP-elevating hormones. ..
  49. Gisler S, Stagljar I, Traebert M, Bacic D, Biber J, Murer H. Interaction of the type IIa Na/Pi cotransporter with PDZ proteins. J Biol Chem. 2001;276:9206-13 pubmed
  50. Song G, Leslie K, Barrick S, Bougoin S, Taboas J, Bisello A. EBP50 promotes focal adhesion turnover and vascular smooth muscle cells migration. J Mol Cell Cardiol. 2012;53:809-19 pubmed publisher
    The ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) is a PDZ-containing scaffolding protein that regulates a variety of physiological functions. In the vasculature, EBP50 promotes neointima formation following arterial injury...
  51. Ritter Makinson S, Paquet M, Bogenpohl J, Rodin R, Chris Yun C, Weinman E, et al. Group II metabotropic glutamate receptor interactions with NHERF scaffold proteins: Implications for receptor localization in brain. Neuroscience. 2017;353:58-75 pubmed publisher
  52. Malmberg E, Pelaseyed T, Petersson A, Seidler U, De Jonge H, Riordan J, et al. The C-terminus of the transmembrane mucin MUC17 binds to the scaffold protein PDZK1 that stably localizes it to the enterocyte apical membrane in the small intestine. Biochem J. 2008;410:283-9 pubmed
    ..Furthermore, we showed weak binding of MUC12 to PDZK1, NHERF1 and NHERF2...
  53. Cunningham R, Steplock D, E X, Biswas R, Wang F, Shenolikar S, et al. Adenoviral expression of NHERF-1 in NHERF-1 null mouse renal proximal tubule cells restores Npt2a regulation by low phosphate media and parathyroid hormone. Am J Physiol Renal Physiol. 2006;291:F896-901 pubmed
    ..Together, these results highlight the requirement for NHERF-1 for physiological control of Npt2a trafficking and suggest that the Npt2a/NHERF-1 complex represents a unique PTH-responsive pool of Npt2a in renal microvilli. ..
  54. Li N, Qin J, Lan L, Zhang H, Liu F, Wu Z, et al. PTEN inhibits macrophage polarization from M1 to M2 through CCL2 and VEGF-A reduction and NHERF-1 synergism. Cancer Biol Ther. 2015;16:297-306 pubmed publisher
    ..The results suggest that the activation of PTEN and NHERF-1 may impede the evolution of macrophages beyond the M1 into M2 phenotype in tumor microenvironment. ..
  55. Cunningham R, Brazie M, Kanumuru S, E X, Biswas R, Wang F, et al. Sodium-hydrogen exchanger regulatory factor-1 interacts with mouse urate transporter 1 to regulate renal proximal tubule uric acid transport. J Am Soc Nephrol. 2007;18:1419-25 pubmed
    ..These findings indicate that NHERF-1 exerts a significant effect on the renal tubular reabsorption of uric acid in the mouse by modulating the BBM abundance of mURAT1 and possibly other BBM uric acid transporters. ..
  56. Singh A, Riederer B, Krabbenhöft A, Rausch B, Bonhagen J, Lehmann U, et al. Differential roles of NHERF1, NHERF2, and PDZK1 in regulating CFTR-mediated intestinal anion secretion in mice. J Clin Invest. 2009;119:540-50 pubmed publisher
    ..Heterologous expression studies have demonstrated that the Na+/H+ exchanger regulatory factors, NHERF1, NHERF2, and PDZK1 (NHERF3), modulate CFTR membrane retention, conductivity, and interactions with other ..
  57. Terawaki S, Maesaki R, Hakoshima T. Structural basis for NHERF recognition by ERM proteins. Structure. 2006;14:777-89 pubmed
    ..Our studies provide structural paradigms for versatile ERM linkages between membrane proteins and the cytoskeleton. ..
  58. Wheeler D, Barrick S, Grubisha M, Brufsky A, Friedman P, Romero G. Direct interaction between NHERF1 and Frizzled regulates ?-catenin signaling. Oncogene. 2011;30:32-42 pubmed publisher
    ..We show here that the adaptor protein Na(+)/H(+) exchange regulatory factor1 (NHERF1), a protein abundantly expressed in normal mammary epithelium, regulates Wnt signaling, maintaining low levels of ?..
  59. Xing W, Kim J, Wergedal J, Chen S, Mohan S. Ephrin B1 regulates bone marrow stromal cell differentiation and bone formation by influencing TAZ transactivation via complex formation with NHERF1. Mol Cell Biol. 2010;30:711-21 pubmed publisher
    ..of the TAZ complex with TAZ-specific antibody revealed a protein complex containing ephrin B1, PTPN13, NHERF1, and TAZ in bone marrow stromal (BMS) cells...
  60. Weinman E, Steplock D, Zhang X, Akhter S, Shenolikar S. Molecular cloning of the cDNA and promoter sequences for the mouse sodium-hydrogen exchanger regulatory factor. Biochim Biophys Acta. 1999;1447:71-6 pubmed
  61. Morales F, Hayashi Y, Van Pelt C, Georgescu M. NHERF1/EBP50 controls lactation by establishing basal membrane polarity complexes with prolactin receptor. Cell Death Dis. 2012;3:e391 pubmed publisher
    ..We show here that this process requires NHERF1/EBP50 (Na/H exchanger regulatory factor 1/ERM-binding phosphoprotein 50) expression and that successful lactation ..
  62. Broere N, Hillesheim J, Tuo B, Jorna H, Houtsmuller A, Shenolikar S, et al. Cystic fibrosis transmembrane conductance regulator activation is reduced in the small intestine of Na+/H+ exchanger 3 regulatory factor 1 (NHERF-1)- but Not NHERF-2-deficient mice. J Biol Chem. 2007;282:37575-84 pubmed
  63. Song G, Barrick S, Leslie K, Bauer P, Alonso V, Friedman P, et al. The scaffolding protein EBP50 promotes vascular smooth muscle cell proliferation and neointima formation by regulating Skp2 and p21(cip1). Arterioscler Thromb Vasc Biol. 2012;32:33-41 pubmed publisher
    The Ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) is a scaffolding protein known to regulate ion homeostasis in the kidney and intestine. Previous work showed that EBP50 expression increases after balloon injury in rat carotids...
  64. Aicart Ramos C, Rodríguez Crespo I. Binding of PDZ domains to the carboxy terminus of inducible nitric oxide synthase boosts electron transfer and NO synthesis. FEBS Lett. 2015;589:2207-12 pubmed publisher
    ..Moreover, the interaction of the iNOS C-terminus with the PDZ domains of EBP50 or CAP70 resulted not only in augmented reductase activity and greater NO synthesis but also anticipated the ..
  65. Weinman E, Biswas R, Steplock D, Douglass T, Cunningham R, Shenolikar S. Sodium-hydrogen exchanger regulatory factor 1 (NHERF-1) transduces signals that mediate dopamine inhibition of sodium-phosphate co-transport in mouse kidney. J Biol Chem. 2010;285:13454-60 pubmed publisher
    ..These studies suggest a novel role for the PDZ adapter protein NHERF-1 in coordinating dopamine signals that inhibit renal phosphate transport. ..
  66. Saotome I, Curto M, McClatchey A. Ezrin is essential for epithelial organization and villus morphogenesis in the developing intestine. Dev Cell. 2004;6:855-64 pubmed
    ..These in vivo studies highlight the critical function of Ezrin in the formation of a multicellular epithelium rather than an individual epithelial cell. ..