Gene Symbol: Slc9a3
Description: solute carrier family 9 (sodium/hydrogen exchanger), member 3
Alias: 9030624O13Rik, AI930210, NHE-3, NHE3, sodium/hydrogen exchanger 3
Species: mouse
Products:     Slc9a3

Top Publications

  1. du Cheyron D, Chalumeau C, Defontaine N, Klein C, Kellermann O, Paillard M, et al. Angiotensin II stimulates NHE3 activity by exocytic insertion of the transporter: role of PI 3-kinase. Kidney Int. 2003;64:939-49 pubmed
    Low-concentration angiotensin II (Ang II) stimulates Na+/H+ exchanger 3 (NHE3) activity in renal proximal tubule mainly via angiotensin II type 1 (AT1) receptors...
  2. Schultheis P, Clarke L, Meneton P, Miller M, Soleimani M, Gawenis L, et al. Renal and intestinal absorptive defects in mice lacking the NHE3 Na+/H+ exchanger. Nat Genet. 1998;19:282-5 pubmed
    NHE3 is one of five plasma membrane Na+/H+ exchangers and is encoded by the mouse gene Slc9a3. It is expressed on apical membranes of renal proximal tubule and intestinal epithelial cells and is thought to play a major role in NaCl and ..
  3. Saifur Rohman M, Emoto N, Nonaka H, Okura R, Nishimura M, Yagita K, et al. Circadian clock genes directly regulate expression of the Na(+)/H(+) exchanger NHE3 in the kidney. Kidney Int. 2005;67:1410-9 pubmed
    ..The mRNA expression profiles of NHE3 and clock genes were examined in mice and rat kidneys...
  4. Brooks H, Sorensen A, Terris J, Schultheis P, Lorenz J, Shull G, et al. Profiling of renal tubule Na+ transporter abundances in NHE3 and NCC null mice using targeted proteomics. J Physiol. 2001;530:359-66 pubmed
    The Na+-H+ exchanger NHE3 and the thiazide-sensitive Na+-Cl- cotransporter NCC are the major apical sodium transporters in the proximal convoluted tubule and the distal convoluted tubule of the kidney, respectively...
  5. Noonan W, Woo A, Nieman M, Prasad V, Schultheis P, Shull G, et al. Blood pressure maintenance in NHE3-deficient mice with transgenic expression of NHE3 in small intestine. Am J Physiol Regul Integr Comp Physiol. 2005;288:R685-91 pubmed
    b>NHE3 Na(+)/H(+) exchanger knockout (Nhe3(-/-)) mice have severe absorptive deficits in the kidney proximal tubule and intestinal tract...
  6. Amlal H, Ledoussal C, Sheriff S, Shull G, Soleimani M. Downregulation of renal AQP2 water channel and NKCC2 in mice lacking the apical Na+-H+ exchanger NHE3. J Physiol. 2003;553:511-22 pubmed
    The apical Na+-H+ exchanger NHE3 plays an important role in fluid reabsorption in the proximal tubule. However, whether its deletion alters the salt and water transport in the distal nephron remains unknown...
  7. Luo X, Choi J, Ko S, Pushkin A, Kurtz I, Ahn W, et al. HCO3- salvage mechanisms in the submandibular gland acinar and duct cells. J Biol Chem. 2001;276:9808-16 pubmed
    ..H(+) and HCO3- transport mechanisms in the submandibular salivary gland (SMG) ducts of wild type, NHE2-/-, NHE3-/-, and NHE2-/-;NHE3-/- double knock-out mice...
  8. Chou S, Hsu K, Otsu W, Hsu Y, Luo Y, Yeh C, et al. CLIC4 regulates apical exocytosis and renal tube luminogenesis through retromer- and actin-mediated endocytic trafficking. Nat Commun. 2016;7:10412 pubmed publisher
    ..CLIC4 selectively modulates retromer-mediated apical transport by negatively regulating the formation of branched actin on early endosomes. ..
  9. Gisler S, Stagljar I, Traebert M, Bacic D, Biber J, Murer H. Interaction of the type IIa Na/Pi cotransporter with PDZ proteins. J Biol Chem. 2001;276:9206-13 pubmed

More Information


  1. Hatanaka M, Kaimori J, Yamamoto S, Matsui I, Hamano T, Takabatake Y, et al. Azilsartan Improves Salt Sensitivity by Modulating the Proximal Tubular Na+-H+ Exchanger-3 in Mice. PLoS ONE. 2016;11:e0147786 pubmed publisher
    ..Immunofluorescence and western blotting showed lower levels of Na+-H+ exchanger-3 (NHE3) protein (the major sodium transporter in renal proximal tubules) in the azilsartan group, but not in the ..
  2. Bailey M, Giebisch G, Abbiati T, Aronson P, Gawenis L, Shull G, et al. NHE2-mediated bicarbonate reabsorption in the distal tubule of NHE3 null mice. J Physiol. 2004;561:765-75 pubmed
    b>NHE3(-/-) mice display a profound defect in proximal tubule bicarbonate reabsorption but are only mildly acidotic owing to reduced glomerular filtration rate and enhanced H(+) secretion in distal nephron segments...
  3. Chen Y, Wu S, Tian Y, Kong J. Phosphorylation and subcellular localization of Na+/H+ exchanger isoform 3 (NHE3) are associated with altered gallbladder absorptive function after formation of cholesterol gallstones. J Physiol Biochem. 2017;73:133-139 pubmed publisher
    ..We conclude that increased phosphorylation of NHE3 following gallstone formation leads to turnover of the exchanger, resulting in decreased gallbladder concentrating function. ..
  4. Chen T, Kocinsky H, Cha B, Murtazina R, Yang J, Tse C, et al. Cyclic GMP kinase II (cGKII) inhibits NHE3 by altering its trafficking and phosphorylating NHE3 at three required sites: identification of a multifunctional phosphorylation site. J Biol Chem. 2015;290:1952-65 pubmed publisher
    The epithelial brush-border Na(+)/H(+) exchanger NHE3 is acutely inhibited by cGKII/cGMP, but how cGKII inhibits NHE3 is unknown...
  5. Du Z, Yan Q, Duan Y, Weinbaum S, Weinstein A, Wang T. Axial flow modulates proximal tubule NHE3 and H-ATPase activities by changing microvillus bending moments. Am J Physiol Renal Physiol. 2006;290:F289-96 pubmed flow-dependent variations in microvillous torque (bending moment); 2) both luminal membrane Na(+)/H+ exchange (NHE3) and H(+)-ATPase activity are modulated by axial flow; and 3) paracellular permeabilities contribute to the flux ..
  6. Walker N, Simpson J, Yen P, Gill R, Rigsby E, Brazill J, et al. Down-regulated in adenoma Cl/HCO3 exchanger couples with Na/H exchanger 3 for NaCl absorption in murine small intestine. Gastroenterology. 2008;135:1645-1653.e3 pubmed publisher
    ..NaCl absorption involves coupling of Cl(-)/HCO(3)(-) exchanger(s) primarily with Na(+)/H(+) exchanger 3 (Nhe3) at the apical membrane of intestinal epithelia...
  7. Li J, Hatano R, Xu S, Wan L, Yang L, Weinstein A, et al. Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl- cotransporter activity and expression in male and female mice. Am J Physiol Renal Physiol. 2017;313:F505-F513 pubmed publisher
    ..There were no gender differences in the expression of the Na+/H+ exchanger (NHE3) in WT; NHE3 protein decreased to similar extents in male and female KO animals, suggesting AT1a-..
  8. Li H, Du Z, Barone S, Rubera I, McDonough A, Tauc M, et al. Proximal tubule specific knockout of the Na?/H? exchanger NHE3: effects on bicarbonate absorption and ammonium excretion. J Mol Med (Berl). 2013;91:951-63 pubmed publisher
    The existing NHE3 knockout mouse has significant intestinal electrolyte absorption defects, making this model unsuitable for the examination of the role of proximal tubule NHE3 in pathophysiologic states in vivo...
  9. Rievaj J, Pan W, Cordat E, Alexander R. The Na?/H? exchanger isoform 3 is required for active paracellular and transcellular Ca²? transport across murine cecum. Am J Physiol Gastrointest Liver Physiol. 2013;305:G303-13 pubmed publisher
    ..Consequently, we hypothesized that Na? absorption mediates Ca²? flux. NHE3 is central to intestinal Na? absorption...
  10. Ledoussal C, Woo A, Miller M, Shull G. Loss of the NHE2 Na(+)/H(+) exchanger has no apparent effect on diarrheal state of NHE3-deficient mice. Am J Physiol Gastrointest Liver Physiol. 2001;281:G1385-96 pubmed
    The expression of NHE2 and NHE3 on intestinal-brush border membranes suggests that both Na(+)/H(+) exchangers serve absorptive functions...
  11. Gawenis L, Stien X, Shull G, Schultheis P, Woo A, Walker N, et al. Intestinal NaCl transport in NHE2 and NHE3 knockout mice. Am J Physiol Gastrointest Liver Physiol. 2002;282:G776-84 pubmed
    ..To investigate the contribution of the apical membrane NHEs, NHE2 and NHE3, to electroneutral NaCl absorption, we measured radioisotopic Na(+) and Cl(-) flux across isolated jejuna from wild-..
  12. Xu H, Li J, Chen R, Zhang B, Wang C, King N, et al. NHE2X3 DKO mice exhibit gender-specific NHE8 compensation. Am J Physiol Gastrointest Liver Physiol. 2011;300:G647-53 pubmed publisher
    ..Intestinal expression of NHE8 is significantly higher than that of NHE2 and NHE3 at a young age, suggesting that NHE8 is an important player for intestinal sodium absorption during early ..
  13. Charoenphandhu N, Kraidith K, Lertsuwan K, Sripong C, Suntornsaratoon P, Svasti S, et al. Na+/H+ exchanger 3 inhibitor diminishes hepcidin-enhanced duodenal calcium transport in hemizygous ?-globin knockout thalassemic mice. Mol Cell Biochem. 2017;427:201-208 pubmed publisher
    ..Interestingly, the selective NHE3 inhibitor, 100 nM tenapanor, markedly inhibited hepcidin-enhanced calcium transport...
  14. Melvin J, Park K, Richardson L, Schultheis P, Shull G. Mouse down-regulated in adenoma (DRA) is an intestinal Cl(-)/HCO(3)(-) exchanger and is up-regulated in colon of mice lacking the NHE3 Na(+)/H(+) exchanger. J Biol Chem. 1999;274:22855-61 pubmed
    ..The expression of mDRA mRNA was modestly up-regulated in the colon of mice lacking the NHE3 Na(+)/H(+) exchanger...
  15. Engevik M, Aihara E, Montrose M, Shull G, Hassett D, Worrell R. Loss of NHE3 alters gut microbiota composition and influences Bacteroides thetaiotaomicron growth. Am J Physiol Gastrointest Liver Physiol. 2013;305:G697-711 pubmed publisher
    ..Na(+)/H(+)-exchanger isoform 3 (NHE3), a target of C. difficile toxin B, plays an integral role in intestinal Na(+) absorption...
  16. Weis V, Knowles B, Choi E, Goldstein A, Williams J, Manning E, et al. Loss of MYO5B in mice recapitulates Microvillus Inclusion Disease and reveals an apical trafficking pathway distinct to neonatal duodenum. Cell Mol Gastroenterol Hepatol. 2016;2:131-157 pubmed
    ..However, the diarrheal pathology after MYO5B loss is due to deficits in transporter presentation at the apical membrane in duodenal enterocytes. ..
  17. Murtazina R, Kovbasnjuk O, Chen T, Zachos N, Chen Y, Kocinsky H, et al. NHERF2 is necessary for basal activity, second messenger inhibition, and LPA stimulation of NHE3 in mouse distal ileum. Am J Physiol Cell Physiol. 2011;301:C126-36 pubmed publisher
    ..Na(+)/H(+) exchanger (NHE) regulatory factor 2 (NHERF2) is necessary for multiple aspects of acute regulation of NHE3 in intact mouse small intestine, distal ileal NHE3 activity was determined using two-photon microscopy/SNARF-4F in ..
  18. Pan W, Borovac J, Spicer Z, Hoenderop J, Bindels R, Shull G, et al. The epithelial sodium/proton exchanger, NHE3, is necessary for renal and intestinal calcium (re)absorption. Am J Physiol Renal Physiol. 2012;302:F943-56 pubmed publisher
    ..Although the epithelial sodium/proton exchanger, NHE3, mediates apical sodium entry at both these sites, its role in Ca(2+) homeostasis remains unclear...
  19. Choijookhuu N, Sato Y, Nishino T, Endo D, Hishikawa Y, Koji T. Estrogen-dependent regulation of sodium/hydrogen exchanger-3 (NHE3) expression via estrogen receptor ? in proximal colon of pregnant mice. Histochem Cell Biol. 2012;137:575-87 pubmed publisher
    ..Expression of NHE3, the gene that harbors estrogen response element, examined by immunohistochemistry and western blotting, was ..
  20. Singh V, Raheja G, Borthakur A, Kumar A, Gill R, Alakkam A, et al. Lactobacillus acidophilus upregulates intestinal NHE3 expression and function. Am J Physiol Gastrointest Liver Physiol. 2012;303:G1393-401 pubmed publisher
    ..However, the effects of LA on NHE3, the Na(+)/H(+) exchanger involved in the coupled electroneutral NaCl absorption, are not known...
  21. Quigley R, Chakravarty S, Zhao X, Imig J, Capdevila J. Increased renal proximal convoluted tubule transport contributes to hypertension in Cyp4a14 knockout mice. Nephron Physiol. 2009;113:p23-8 pubmed publisher
    ..Expression of the sodium-hydrogen exchanger 3 (NHE3), the predominant transporter responsible for sodium transport in this segment, was measured by immunoblot...
  22. Duan Y, Weinstein A, Weinbaum S, Wang T. Shear stress-induced changes of membrane transporter localization and expression in mouse proximal tubule cells. Proc Natl Acad Sci U S A. 2010;107:21860-5 pubmed publisher
    ..single proximal tubule showed that flow-dependent Na(+) and HCO(3)(-) reabsorption is due to a modulation of both NHE3 and vacuolar H(+)-ATPase (V-ATPase) activity...
  23. Murtazina R, Kovbasnjuk O, Zachos N, Li X, Chen Y, Hubbard A, et al. Tissue-specific regulation of sodium/proton exchanger isoform 3 activity in Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) null mice. cAMP inhibition is differentially dependent on NHERF1 and exchange protein directly activated by cAMP in ileum ve. J Biol Chem. 2007;282:25141-51 pubmed
    ..domain containing protein Na(+)/H(+) Exchanger Regulatory Factor 1 (NHERF1) binds to Na(+)/H(+) exchanger 3 (NHE3) and is associated with the brush border (BB) membrane of murine kidney and small intestine...
  24. Sandu C, Artunc F, Palmada M, Rexhepaj R, Grahammer F, Hussain A, et al. Impaired intestinal NHE3 activity in the PDK1 hypomorphic mouse. Am J Physiol Gastrointest Liver Physiol. 2006;291:G868-76 pubmed
    ..stimulating effect of serum- and glucocorticoid-inducible kinase (SGK)1 on the activity of the Na+/H+ exchanger (NHE3)...
  25. Evans R, Bell S, Schultheis P, Shull G, Melvin J. Targeted disruption of the Nhe1 gene prevents muscarinic agonist-induced up-regulation of Na(+)/H(+) exchange in mouse parotid acinar cells. J Biol Chem. 1999;274:29025-30 pubmed
    ..Although multiple NHE isoforms (NHE1, NHE2, and NHE3) have been identified in salivary glands, little is known about their specific function(s) in resting and secreting ..
  26. Fenton R, Poulsen S, de la Mora Chavez S, Soleimani M, Dominguez Rieg J, Rieg T. Renal tubular NHE3 is required in the maintenance of water and sodium chloride homeostasis. Kidney Int. 2017;92:397-414 pubmed publisher
    The sodium/proton exchanger isoform 3 (NHE3) is expressed in the intestine and the kidney, where it facilitates sodium (re)absorption and proton secretion...
  27. Thomson R, Wang T, Thomson B, Tarrats L, Girardi A, Mentone S, et al. Role of PDZK1 in membrane expression of renal brush border ion exchangers. Proc Natl Acad Sci U S A. 2005;102:13331-6 pubmed
    Na-H exchanger NHE3 and Cl-anion exchanger CFEX (SLC26A6, PAT1) play principal roles in the reabsorption of Na and Cl in the proximal tubule of the mammalian kidney...
  28. Grahammer F, Henke G, Sandu C, Rexhepaj R, Hussain A, Friedrich B, et al. Intestinal function of gene-targeted mice lacking serum- and glucocorticoid-inducible kinase 1. Am J Physiol Gastrointest Liver Physiol. 2006;290:G1114-23 pubmed
    ..SGK1) to stimulate intestinal Na(+)-coupled glucose cotransporter 1 (SGLT1) and intestinal Na(+)/H(+) exchanger 3 (NHE3). The present study explored the contribution of SGK1 to the regulation of intestinal transport in vivo...
  29. Teng C, Li Y, Stockton P, Foley J. Fasting induces the expression of PGC-1? and ERR isoforms in the outer stripe of the outer medulla (OSOM) of the mouse kidney. PLoS ONE. 2011;6:e26961 pubmed publisher
    ..Taken together, our data suggest that fasting activates the energy balance program in the OSOM of the kidney. ..
  30. Choi J, Shah M, Lee M, Schultheis P, Shull G, Muallem S, et al. Novel amiloride-sensitive sodium-dependent proton secretion in the mouse proximal convoluted tubule. J Clin Invest. 2000;105:1141-6 pubmed
    ..Several NHE isoforms have been cloned, but only NHE3 and NHE2 are known to be present on the apical membrane of the PCT...
  31. Woo A, Noonan W, Schultheis P, Neumann J, Manning P, Lorenz J, et al. Renal function in NHE3-deficient mice with transgenic rescue of small intestinal absorptive defect. Am J Physiol Renal Physiol. 2003;284:F1190-8 pubmed
    The degree to which loss of the NHE3 Na(+)/H(+) exchanger in the kidney contributes to impaired Na(+)-fluid volume homeostasis in NHE3-deficient (Nhe3(-/-)) mice is unclear because of the coexisting intestinal absorptive defect...
  32. Zhou Q, Clarke L, Nie R, Carnes K, Lai L, Lien Y, et al. Estrogen action and male fertility: roles of the sodium/hydrogen exchanger-3 and fluid reabsorption in reproductive tract function. Proc Natl Acad Sci U S A. 2001;98:14132-7 pubmed
    ..It is shown that estrogen regulates expression of the Na(+)/H(+) exchanger-3 (NHE3) and the rate of (22)Na(+) transport, sensitive to an NHE3 inhibitor...
  33. Yeruva S, Chodisetti G, Luo M, Chen M, Cinar A, Ludolph L, et al. Evidence for a causal link between adaptor protein PDZK1 downregulation and Na⁺/H⁺ exchanger NHE3 dysfunction in human and murine colitis. Pflugers Arch. 2015;467:1795-807 pubmed publisher
    A dysfunction of the Na(+)/H(+) exchanger isoform 3 (NHE3) significantly contributes to the reduced salt absorptive capacity of the inflamed intestine...
  34. Li X, Shull G, Miguel Qin E, Zhuo J. Role of the Na+/H+ exchanger 3 in angiotensin II-induced hypertension. Physiol Genomics. 2015;47:479-87 pubmed publisher
    ..The present study tested the hypothesis that the Na(+)/H(+) exchanger 3 (NHE3) is required for ANG II-induced hypertension in mice...
  35. Clayburgh D, Musch M, Leitges M, Fu Y, Turner J. Coordinated epithelial NHE3 inhibition and barrier dysfunction are required for TNF-mediated diarrhea in vivo. J Clin Invest. 2006;116:2682-94 pubmed
    ..but not LIGHT, inhibited Na+ absorption due to TNF-induced internalization of the brush border Na+/H+ exchanger NHE3. LIGHT did not cause NHE3 internalization...
  36. Hillesheim J, Riederer B, Tuo B, Chen M, Manns M, Biber J, et al. Down regulation of small intestinal ion transport in PDZK1- (CAP70/NHERF3) deficient mice. Pflugers Arch. 2007;454:575-86 pubmed
    ..transmembrane conductance regulator (CFTR), the anion exchangers SLC26A3 and SLC26A6 and the Na(+)/H(+) exchanger NHE3, all of which are major transport proteins for intestinal anion secretion and salt absorption...
  37. Laubitz D, Larmonier C, Bai A, Midura Kiela M, Lipko M, Thurston R, et al. Colonic gene expression profile in NHE3-deficient mice: evidence for spontaneous distal colitis. Am J Physiol Gastrointest Liver Physiol. 2008;295:G63-G77 pubmed publisher
    Na+/H+ exchanger 3 (NHE3) provides a major route for intestinal Na+ absorption...
  38. Singh A, Liu Y, Riederer B, Engelhardt R, Thakur B, Soleimani M, et al. Molecular transport machinery involved in orchestrating luminal acid-induced duodenal bicarbonate secretion in vivo. J Physiol. 2013;591:5377-91 pubmed publisher
    ..Using genetically engineered mouse models lacking Scl26a3, Slc26a6, Slc26a9 or Slc9a3 (NHE3), the study was carried out to assess the role of these transporters in mediating the protective duodenal ..
  39. Xia W, Yu Q, Riederer B, Singh A, Engelhardt R, Yeruva S, et al. The distinct roles of anion transporters Slc26a3 (DRA) and Slc26a6 (PAT-1) in fluid and electrolyte absorption in the murine small intestine. Pflugers Arch. 2014;466:1541-56 pubmed
    ..was performed in anaesthetised wild type (WT) as well as in mice deficient in DRA, PAT-1, Na+/H+ exchanger 3 (NHE3) or NHE2, and in carbonic anhydrase II (CAII). Unbuffered saline (pH 7...
  40. Magalhães D, Cabral J, Soares da Silva P, Magro F. Role of epithelial ion transports in inflammatory bowel disease. Am J Physiol Gastrointest Liver Physiol. 2016;310:G460-76 pubmed publisher
    ..In addition, the downregulation of apical NHE and ENaC and the upregulation of apical large-conductance KC all contribute to the IBD-associated diarrhea by lowering sodium absorption and/or increasing potassium secretion. ..
  41. Bacic D, Kaissling B, McLeroy P, Zou L, Baum M, Moe O. Dopamine acutely decreases apical membrane Na/H exchanger NHE3 protein in mouse renal proximal tubule. Kidney Int. 2003;64:2133-41 pubmed
    ..Proximal tubule natriuresis is effected through inhibition of the apical membrane Na/H exchanger NHE3. We examined whether dopamine directly and acutely decreases apical membrane NHE3 protein using renal tissue in two ..
  42. Pathak B, Shull G, Jenkins N, Copeland N. Mouse chromosomal location of four Na/H exchanger isoform genes. Genomics. 1996;31:261-3 pubmed
    The Na/H exchanger genes Slc9a1, Slc9a2, Slc9a3, and Slc9a4 have been mapped in the mouse using an interspecific backcross panel...
  43. Pasham V, Rotte A, Gu S, Yang W, Bhandaru M, Rexhepaj R, et al. Upregulation of intestinal NHE3 following saline ingestion. Kidney Blood Press Res. 2013;37:48-57 pubmed publisher
    ..SGK1 is in turn a powerful stimulator of the intestinal Na(+)/H(+) exchanger NHE3. The present study was thus performed to elucidate, whether the NaCl content of beverages influences NHE3 activity...
  44. Larmonier C, Laubitz D, Thurston R, Bucknam A, Hill F, Midura Kiela M, et al. NHE3 modulates the severity of colitis in IL-10-deficient mice. Am J Physiol Gastrointest Liver Physiol. 2011;300:G998-G1009 pubmed publisher
    b>NHE3, the major intestinal Na(+)/H(+) exchanger, was shown to be downregulated and/or inhibited in patients with inflammatory bowel disease (IBD), a phenomenon believed to contribute to inflammation-associated diarrhea...
  45. Mennone A, Biemesderfer D, Negoianu D, Yang C, Abbiati T, Schultheis P, et al. Role of sodium/hydrogen exchanger isoform NHE3 in fluid secretion and absorption in mouse and rat cholangiocytes. Am J Physiol Gastrointest Liver Physiol. 2001;280:G247-54 pubmed
    ..The isoform NHE3 has been localized to apical surfaces of epithelia and in some tissues may facilitate the absorption of NaCl...
  46. Tamimi R, Steingrimsson E, Montgomery Dyer K, Copeland N, Jenkins N, Tapscott S. NEUROD2 and NEUROD3 genes map to human chromosomes 17q12 and 5q23-q31 and mouse chromosomes 11 and 13, respectively. Genomics. 1997;40:355-7 pubmed
    ..NEUROD2 maps to human chromosome 17q12 and mouse chromosome 11. NEUROD3 maps to human chromosome 5q23-q31 and mouse chromosome 13. ..
  47. Schultheis P, Clarke L, Meneton P, Harline M, Boivin G, Stemmermann G, et al. Targeted disruption of the murine Na+/H+ exchanger isoform 2 gene causes reduced viability of gastric parietal cells and loss of net acid secretion. J Clin Invest. 1998;101:1243-53 pubmed
  48. Wang Y, Lin Y, Wu Y, Chen Y, Lin Y, Cheng C, et al. Loss of SLC9A3 decreases CFTR protein and causes obstructed azoospermia in mice. PLoS Genet. 2017;13:e1006715 pubmed publisher
    ..Some patients have a single copy deletion of the solute carrier family 9 isoform 3 (SLC9A3) gene...
  49. Lee M, Ahn W, Choi J, Luo X, Seo J, Schultheis P, et al. Na(+)-dependent transporters mediate HCO(3)(-) salvage across the luminal membrane of the main pancreatic duct. J Clin Invest. 2000;105:1651-8 pubmed
    ..H(+) transporters, we characterized H(+) efflux mechanisms in the pancreatic duct in wild-type, NHE2(-/-), and NHE3(-/-) mice...
  50. Nishinaga H, Komatsu R, Doi M, Fustin J, Yamada H, Okura R, et al. Circadian expression of the Na+/H+ exchanger NHE3 in the mouse renal medulla. Biomed Res. 2009;30:87-93 pubmed
    Renal tubular NHE3, the Na+/H+ exchanger, is a critical enzyme for electrolyte and acid-base homeostasis in the kidney...
  51. Bosse A, Stoykova A, Nieselt Struwe K, Chowdhury K, Copeland N, Jenkins N, et al. Identification of a novel mouse Iroquois homeobox gene, Irx5, and chromosomal localisation of all members of the mouse Iroquois gene family. Dev Dyn. 2000;218:160-74 pubmed
    ..Together, with the data obtained from the chromosomal mapping analysis, the results indicate that these genes have appeared in vertebrates during evolution as a result of gene duplication. ..
  52. Petrovic S, Barone S, Wang Z, McDonough A, Amlal H, Soleimani M. Slc26a6 (PAT1) deletion downregulates the apical Na+/H+ exchanger in the straight segment of the proximal tubule. Am J Nephrol. 2008;28:330-8 pubmed
    ..The purpose of the present study was to examine the effect of Slc26a6 deletion on the apical Na+/H+ exchanger 3 (NHE3) in the straight segment (S3) of the proximal tubule, which is the major site for the reabsorption of filtered ..
  53. Wang T, Yang C, Abbiati T, Shull G, Giebisch G, Aronson P. Essential role of NHE3 in facilitating formate-dependent NaCl absorption in the proximal tubule. Am J Physiol Renal Physiol. 2001;281:F288-92 pubmed
    ..The formate recycling process requires H(+) secretion. Although Na(+)-H(+) exchanger isoform NHE3 accounts for the largest component of H(+) secretion in the proximal tubule, 40-50% of the rates of HCO absorption ..
  54. Park J, Ryu J, Yun S, Kim M, Han H. Fibronectin stimulates migration through lipid raft dependent NHE-1 activation in mouse embryonic stem cells: involvement of RhoA, Ca(2+)/CaM, and ERK. Biochim Biophys Acta. 2012;1820:1618-27 pubmed publisher
    ..Thus, this study analyzed the relationship between FN and NHE in regulating the migration of mouse ESCs and their related signaling pathways. ..
  55. Shawki A, Engevik M, Kim R, Knight P, Baik R, Anthony S, et al. Intestinal brush-border Na+/H+ exchanger-3 drives H+-coupled iron absorption in the mouse. Am J Physiol Gastrointest Liver Physiol. 2016;311:G423-30 pubmed publisher
    ..exchanger (NHE) isoforms by examining iron homeostasis and intestinal iron handling in mice lacking NHE2 or NHE3. We observed modestly depleted liver iron stores in NHE2-null (NHE2(-/-)) mice stressed on a low-iron diet but no ..
  56. Sultan A, Luo M, Yu Q, Riederer B, Xia W, Chen M, et al. Differential association of the Na+/H+ Exchanger Regulatory Factor (NHERF) family of adaptor proteins with the raft- and the non-raft brush border membrane fractions of NHE3. Cell Physiol Biochem. 2013;32:1386-402 pubmed
    Trafficking, brush border membrane (BBM) retention, and signal-specific regulation of the Na+/H+ exchanger NHE3 is regulated by the Na+/H+ Exchanger Regulatory Factor (NHERF) family of PDZ-adaptor proteins, which enable the formation of ..
  57. Gawenis L, Hut H, Bot A, Shull G, de Jonge H, Stien X, et al. Electroneutral sodium absorption and electrogenic anion secretion across murine small intestine are regulated in parallel. Am J Physiol Gastrointest Liver Physiol. 2004;287:G1140-9 pubmed
    ..Under basal conditions, electroneutral Na(+) absorption mediated by Na(+)/H(+) exchanger 3 (NHE3) predominates; under stimulated conditions, increased anion secretion mediated by CFTR occurs concurrently with ..
  58. Shull G, Miller M, Schultheis P. Lessons from genetically engineered animal models VIII. Absorption and secretion of ions in the gastrointestinal tract. Am J Physiol Gastrointest Liver Physiol. 2000;278:G185-90 pubmed
    ..This approach is leading to a clearer understanding of the functions of these transporters in the living animal...
  59. Boedtkjer E, Praetorius J, Matchkov V, Stankevicius E, Mogensen S, Füchtbauer A, et al. Disruption of Na+,HCO?? cotransporter NBCn1 (slc4a7) inhibits NO-mediated vasorelaxation, smooth muscle Ca²? sensitivity, and hypertension development in mice. Circulation. 2011;124:1819-29 pubmed publisher
    ..Intracellular acidification of smooth muscle and endothelial cells after knockout of NBCn1 inhibits NO-mediated and rho-kinase-dependent signaling in isolated arteries and perturbs blood pressure regulation. ..
  60. Praetorius J, Andreasen D, Jensen B, Ainsworth M, Friis U, Johansen T. NHE1, NHE2, and NHE3 contribute to regulation of intracellular pH in murine duodenal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2000;278:G197-206 pubmed
    ..NHE1, NHE2, and NHE3 were expressed at the mRNA level (RT-PCR) as well as at the protein level (Western blot analysis)...
  61. Siyanov V, Baltz J. NHE1 is the sodium-hydrogen exchanger active in acute intracellular pH regulation in preimplantation mouse embryos. Biol Reprod. 2013;88:157 pubmed publisher
    ..membrane NHE isoforms, mouse oocytes and preimplantation embryos express mRNAs encoding NHE1 (SLC9A1), NHE3 (SLC9A3), and NHE4 (SLC9A4), with higher mRNA levels for each in oocytes through one-cell stage embryos and lower levels ..
  62. Baum M, Twombley K, Gattineni J, Joseph C, Wang L, Zhang Q, et al. Proximal tubule Na+/H+ exchanger activity in adult NHE8-/-, NHE3-/-, and NHE3-/-/NHE8-/- mice. Am J Physiol Renal Physiol. 2012;303:F1495-502 pubmed publisher
    b>NHE3 is the predominant Na(+)/H(+) exchanger on the brush-border membrane (BBM) of the proximal tubule in adults. However, NHE3 null mice still have significant renal BBM Na(+)/H(+) activity...
  63. Lorenz J, Schultheis P, Traynor T, Shull G, Schnermann J. Micropuncture analysis of single-nephron function in NHE3-deficient mice. Am J Physiol. 1999;277:F447-53 pubmed
    The Na/H exchanger isoform 3 (NHE3) is expressed in the proximal tubule and thick ascending limb and contributes to the reabsorption of fluid and electrolytes in these segments...
  64. Catalán M, Nakamoto T, Gonzalez Begne M, Camden J, Wall S, Clarke L, et al. Cftr and ENaC ion channels mediate NaCl absorption in the mouse submandibular gland. J Physiol. 2010;588:713-24 pubmed publisher
    ..In summary, our results demonstrate that NaCl absorption requires and is likely to be mediated by functionally dependent Cftr and ENaC channels localized to the apical membranes of mouse salivary gland duct cells. ..
  65. Good D, George T, Watts B. Nongenomic regulation by aldosterone of the epithelial NHE3 Na(+)/H(+) exchanger. Am J Physiol Cell Physiol. 2006;290:C757-63 pubmed
    ..In the MTAL, apical NHE3 mediates H(+) secretion necessary for HCO(3)(-) absorption; basolateral NHE1 influences HCO(3)(-) absorption by ..
  66. Jeong H, Jeon U, Koo B, Kim W, Im S, Shin J, et al. Inactivation of Notch signaling in the renal collecting duct causes nephrogenic diabetes insipidus in mice. J Clin Invest. 2009;119:3290-300 pubmed publisher
  67. Lin Z, Jin S, Duan X, Wang T, Martini S, Hulamm P, et al. Chloride channel (Clc)-5 is necessary for exocytic trafficking of Na+/H+ exchanger 3 (NHE3). J Biol Chem. 2011;286:22833-45 pubmed publisher
    ..Absence of ClC-5 in mice is associated with reduced surface expression of NHE3 in proximal tubules. The molecular basis for this change is not fully understood...
  68. Cinar A, Chen M, Riederer B, Bachmann O, Wiemann M, Manns M, et al. NHE3 inhibition by cAMP and Ca2+ is abolished in PDZ-domain protein PDZK1-deficient murine enterocytes. J Physiol. 2007;581:1235-46 pubmed
    The PDZ-binding protein PDZK1 (NHERF3/CAP70/PDZ-dc-1) in vitro binds to NHE3, but its role in the regulation of NHE3 activity in native enterocytes is unknown...
  69. He P, Zhao L, Zhu L, Weinman E, De Giorgio R, Koval M, et al. Restoration of Na+/H+ exchanger NHE3-containing macrocomplexes ameliorates diabetes-associated fluid loss. J Clin Invest. 2015;125:3519-31 pubmed publisher
    ..We found that the expression of Na+/H+ exchanger NHE3 and several scaffold proteins, including NHE3 regulatory factors (NHERFs), inositol trisphosphate (IP₃) ..
  70. Nakamura K, Stokes J, McCray P. Endogenous and exogenous glucocorticoid regulation of ENaC mRNA expression in developing kidney and lung. Am J Physiol Cell Physiol. 2002;283:C762-72 pubmed
  71. Shenolikar S, Voltz J, Minkoff C, Wade J, Weinman E. Targeted disruption of the mouse NHERF-1 gene promotes internalization of proximal tubule sodium-phosphate cotransporter type IIa and renal phosphate wasting. Proc Natl Acad Sci U S A. 2002;99:11470-5 pubmed
    ..were identified as essential factors for protein kinase A-mediated inhibition of the sodium-hydrogen exchanger, NHE3. NHERF-1 and NHERF-2 also bound other cellular targets including the sodium-phosphate cotransporter type IIa ..
  72. Larmonier C, Laubitz D, Hill F, Shehab K, Lipinski L, Midura Kiela M, et al. Reduced colonic microbial diversity is associated with colitis in NHE3-deficient mice. Am J Physiol Gastrointest Liver Physiol. 2013;305:G667-77 pubmed publisher
    ..b>NHE3 expression is essential for the maintenance of the epithelial barrier function...
  73. Singh A, Riederer B, Chen M, Xiao F, Krabbenhöft A, Engelhardt R, et al. The switch of intestinal Slc26 exchangers from anion absorptive to HCOFormula secretory mode is dependent on CFTR anion channel function. Am J Physiol Cell Physiol. 2010;298:C1057-65 pubmed publisher
    ..The duodena of anesthetized CFTR-, NHE3-, Slc26a6-, and Scl26a3-deficient mice and wild-type (WT) littermates were perfused, and duodenal bicarbonate (HCO(..
  74. Beck F, Neuhofer W, Dörge A, Giebisch G, Wang T. Intracellular Na concentration and Rb uptake in proximal convoluted tubule cells and abundance of Na/K-ATPase alpha1-subunit in NHE3-/- mice. Pflugers Arch. 2003;446:100-5 pubmed
    ..absorption is greatly reduced in mice in which the gene encoding the Na/H exchanger isoform 3 has been ablated (NHE3-/-)...
  75. Lin S, Yeruva S, He P, Singh A, Zhang H, Chen M, et al. Lysophosphatidic acid stimulates the intestinal brush border Na(+)/H(+) exchanger 3 and fluid absorption via LPA(5) and NHERF2. Gastroenterology. 2010;138:649-58 pubmed publisher
    ..The bulk of sodium and water absorption in the intestine is mediated by Na(+)/H(+) exchanger 3 (NHE3), located in the luminal membrane of enterocytes...