Slc5a7

Summary

Gene Symbol: Slc5a7
Description: solute carrier family 5 (choline transporter), member 7
Alias: CHT1, high affinity choline transporter 1, CHT, hemicholinium-3-sensitive choline transporter, solute carrier family 5 member 7
Species: mouse
Products:     Slc5a7

Top Publications

  1. Bazalakova M, Wright J, Schneble E, McDonald M, Heilman C, Levey A, et al. Deficits in acetylcholine homeostasis, receptors and behaviors in choline transporter heterozygous mice. Genes Brain Behav. 2007;6:411-24 pubmed
    Cholinergic neurons elaborate a hemicholinium-3 (HC-3) sensitive choline transporter (CHT) that mediates presynaptic, high-affinity choline uptake (HACU) in support of acetylcholine (ACh) synthesis and release...
  2. Ferguson S, Bazalakova M, Savchenko V, Tapia J, Wright J, Blakely R. Lethal impairment of cholinergic neurotransmission in hemicholinium-3-sensitive choline transporter knockout mice. Proc Natl Acad Sci U S A. 2004;101:8762-7 pubmed
    ..ACh) synthesis and release is thought to be sustained by a hemicholinium-3-sensitive choline transporter (CHT)...
  3. Wang Y, Cao Z, Newkirk R, Ivy M, Townsel J. Molecular cloning of a cDNA for a putative choline co-transporter from Limulus CNS. Gene. 2001;268:123-31 pubmed
    ..recently reported the successful cloning of the choline co-transporter in Caenorhabditis elegans (CHO-1) and rat (CHT1). We report herein the cloning of the choline co-transporter in the horseshoe crab, Limulus polyphemus...
  4. Miura E, Fukaya M, Sato T, Sugihara K, Asano M, Yoshioka K, et al. Expression and distribution of JNK/SAPK-associated scaffold protein JSAP1 in developing and adult mouse brain. J Neurochem. 2006;97:1431-46 pubmed
    ..Therefore, the characteristic cellular expression and subcellular distribution of JSAP1 might be beneficial for cells to efficiently link external stimuli to the JNK MAPK pathway and other intracellular machineries. ..
  5. Zurkovsky L, Bychkov E, Tsakem E, Siedlecki C, Blakely R, Gurevich E. Cognitive effects of dopamine depletion in the context of diminished acetylcholine signaling capacity in mice. Dis Model Mech. 2013;6:171-83 pubmed publisher
    ..Mice carrying heterozygous deletion of the high-affinity, hemicholinium-3-sensitive choline transporter (CHT(HET)) show reduced levels of acetylcholine throughout the brain...
  6. Cho H, Cargnin F, Kim Y, Lee B, Kwon R, Nam H, et al. Isl1 directly controls a cholinergic neuronal identity in the developing forebrain and spinal cord by forming cell type-specific complexes. PLoS Genet. 2014;10:e1004280 pubmed publisher
  7. Lips K, Luhrmann A, Tschernig T, Stoeger T, Alessandrini F, Grau V, et al. Down-regulation of the non-neuronal acetylcholine synthesis and release machinery in acute allergic airway inflammation of rat and mouse. Life Sci. 2007;80:2263-9 pubmed
    ..Targets of investigation were the high-affinity choline transporter-1 (CHT1), that mediates cellular uptake of choline, the ACh-synthesizing enzyme choline acetyltransferase (ChAT), the ..
  8. Brock M, Nickel A, Madziar B, Blusztajn J, Berse B. Differential regulation of the high affinity choline transporter and the cholinergic locus by cAMP signaling pathways. Brain Res. 2007;1145:1-10 pubmed
    ..acetyltransferase (ChAT), vesicular acetylcholine transporter (VAChT) and the high-affinity choline transporter (CHT). Extracellular factors that regulate CHT expression and their signaling pathways remain poorly characterized...
  9. Xie J, Guo Q. Par-4 inhibits choline uptake by interacting with CHT1 and reducing its incorporation on the plasma membrane. J Biol Chem. 2004;279:28266-75 pubmed
    b>CHT1 is a Na(+)- and Cl(-)-dependent, hemicholinium-3 (HC-3)-sensitive, high affinity choline transporter...

More Information

Publications31

  1. Guermonprez L, O Regan S, Meunier F, Morot Gaudry Talarmain Y. The neuronal choline transporter CHT1 is regulated by immunosuppressor-sensitive pathways. J Neurochem. 2002;82:874-84 pubmed
    ..The Torpedo homologue of the high affinity choline transporter CHT1 was cloned and its activity was reconstituted in Xenopus oocytes...
  2. Okuda T, Okamura M, Kaitsuka C, Haga T, Gurwitz D. Single nucleotide polymorphism of the human high affinity choline transporter alters transport rate. J Biol Chem. 2002;277:45315-22 pubmed
    ..Recently, we succeeded in molecular cloning of the high affinity choline transporter (CHT1), which is specifically expressed in cholinergic neurons...
  3. Berse B, Szczecinska W, Lopez Coviella I, Madziar B, Zemelko V, Kaminski R, et al. Expression of high affinity choline transporter during mouse development in vivo and its upregulation by NGF and BMP-4 in vitro. Brain Res Dev Brain Res. 2005;157:132-40 pubmed
    ..The choline transporter, designated CHT, was recently cloned...
  4. Rassadi S, Krishnaswamy A, PiƩ B, McConnell R, Jacob M, Cooper E. A null mutation for the alpha3 nicotinic acetylcholine (ACh) receptor gene abolishes fast synaptic activity in sympathetic ganglia and reveals that ACh output from developing preganglionic terminals is regulated in an activity-dependent retrograde ma. J Neurosci. 2005;25:8555-66 pubmed
  5. Nassenstein C, Wiegand S, Lips K, Li G, Klein J, Kummer W. Cholinergic activation of the murine trachealis muscle via non-vesicular acetylcholine release involving low-affinity choline transporters. Int Immunopharmacol. 2015;29:173-80 pubmed publisher
    ..Hemicholinium-3, in low concentrations an inhibitor of the high-affinity choline transporter-1 (CHT1), completely abrogated the eserine effects when applied in high concentrations (10(-4)M) pointing towards an ..
  6. Marshall K, Godden E, Yang F, Burgers S, Buck K, Sikela J. In silico discovery of gene-coding variants in murine quantitative trait loci using strain-specific genome sequence databases. Genome Biol. 2002;3:RESEARCH0078 pubmed
    ..As a test of this approach we focused on two QTLs on mouse chromosomes 1 and 13 that are involved in physical dependence on alcohol...
  7. Holmstrand E, Lund D, Cherian A, Wright J, Martin R, Ennis E, et al. Transgenic overexpression of the presynaptic choline transporter elevates acetylcholine levels and augments motor endurance. Neurochem Int. 2014;73:217-28 pubmed publisher
    ..chromosome (BAC)-mediated transgenic methods, we generated mice with integrated additional copies of the mouse Slc5a7 gene. BAC-CHT mice are viable, appear to develop normally, and breed at wild-type (WT) rates...
  8. Cuddy L, Winick Ng W, Rylett R. Regulation of the high-affinity choline transporter activity and trafficking by its association with cholesterol-rich lipid rafts. J Neurochem. 2014;128:725-40 pubmed publisher
    The sodium-coupled, hemicholinium-3-sensitive, high-affinity choline transporter (CHT) is responsible for transport of choline into cholinergic nerve terminals from the synaptic cleft following acetylcholine release and hydrolysis...
  9. Apparsundaram S, Ferguson S, Blakely R. Molecular cloning and characterization of a murine hemicholinium-3-sensitive choline transporter. Biochem Soc Trans. 2001;29:711-6 pubmed
    ..The mCHT cDNA encodes a protein of 580 amino acids with 93% and 98% amino acid identity to human CHT and rat CHT1, respectively...
  10. Iwamoto H, Calcutt M, Blakely R. Differential impact of genetically modulated choline transporter expression on the release of endogenous versus newly synthesized acetylcholine. Neurochem Int. 2016;98:138-45 pubmed publisher
    ..import of choline into cholinergic nerve terminals by the presynaptic, high-affinity choline transporter (CHT, SLC5A7) dictates the capacity for acetylcholine (ACh) synthesis and release...
  11. Dong Y, Dani J, Blakely R. Choline transporter hemizygosity results in diminished basal extracellular dopamine levels in nucleus accumbens and blunts dopamine elevations following cocaine or nicotine. Biochem Pharmacol. 2013;86:1084-8 pubmed publisher
    ..is dictated by the availability and activity of the presynaptic, high-affinity, choline transporter (CHT, SLC5A7) that acquires choline in an activity-dependent matter to sustain ACh synthesis...
  12. Parikh V, St Peters M, Blakely R, Sarter M. The presynaptic choline transporter imposes limits on sustained cortical acetylcholine release and attention. J Neurosci. 2013;33:2326-37 pubmed publisher
    Functional variation in the gene encoding the presynaptic choline transporter (CHT) has been linked to attention-deficit/hyperactivity disorder...
  13. Lund D, Ruggiero A, Ferguson S, Wright J, English B, Reisz P, et al. Motor neuron-specific overexpression of the presynaptic choline transporter: impact on motor endurance and evoked muscle activity. Neuroscience. 2010;171:1041-53 pubmed publisher
    The presynaptic, hemicholinium-3 sensitive, high-affinity choline transporter (CHT) supplies choline for acetylcholine (ACh) synthesis...
  14. Wang Z, Wang B, Yang L, Guo Q, Aithmitti N, Songyang Z, et al. Presynaptic and postsynaptic interaction of the amyloid precursor protein promotes peripheral and central synaptogenesis. J Neurosci. 2009;29:10788-801 pubmed publisher
    ..We postulate that transsynaptic APP interaction modulates its synaptic function and that perturbed APP synaptic adhesion activity may contribute to synaptic dysfunction and AD pathogenesis. ..
  15. Krishnaswamy A, Cooper E. An activity-dependent retrograde signal induces the expression of the high-affinity choline transporter in cholinergic neurons. Neuron. 2009;61:272-86 pubmed publisher
    ..Moreover, we demonstrate with rescue experiments that CHT is induced by signals downstream of postsynaptic activity, converting immature terminals to mature terminals ..
  16. Misawa H, Fujigaya H, Nishimura T, Moriwaki Y, Okuda T, Kawashima K, et al. Aberrant trafficking of the high-affinity choline transporter in AP-3-deficient mice. Eur J Neurosci. 2008;27:3109-17 pubmed publisher
    The high-affinity choline transporter (CHT) is expressed in cholinergic neurons and efficiently transported to axon terminals where it controls the rate-limiting step in acetylcholine synthesis...
  17. Wang B, Yang L, Wang Z, Zheng H. Amyolid precursor protein mediates presynaptic localization and activity of the high-affinity choline transporter. Proc Natl Acad Sci U S A. 2007;104:14140-5 pubmed
    ..APP is essential in regulating the presynaptic expression and activity of the high-affinity choline transporter (CHT), a molecule that mediates the rate-limiting step of cholinergic synaptic transmission in both the neuromuscular ..
  18. Payette D, Xie J, Guo Q. Reduction in CHT1-mediated choline uptake in primary neurons from presenilin-1 M146V mutant knock-in mice. Brain Res. 2007;1135:12-21 pubmed
    ..Neuronal uptake of choline via the high affinity choline transporter (CHT1) is essential for cholinergic neurotransmission...
  19. Gates J, Ferguson S, Blakely R, Apparsundaram S. Regulation of choline transporter surface expression and phosphorylation by protein kinase C and protein phosphatase 1/2A. J Pharmacol Exp Ther. 2004;310:536-45 pubmed
    The Na(+)/Cl(-)-dependent, hemicholinium-3-sensitive choline transporter (CHT) provides choline for acetylcholine biosynthesis. Recent studies show that CHT contains canonical protein kinase C (PKC) serine and threonine residues...
  20. English B, Appalsamy M, Diedrich A, RUGGIERO A, Lund D, Wright J, et al. Tachycardia, reduced vagal capacity, and age-dependent ventricular dysfunction arising from diminished expression of the presynaptic choline transporter. Am J Physiol Heart Circ Physiol. 2010;299:H799-810 pubmed publisher
    ..To date, the cardiovascular impact of diminished choline transporter (CHT) expression has not been directly examined, largely due to the transporter's inaccessibility in vivo...
  21. de Castro B, De Jaeger X, Martins Silva C, Lima R, Amaral E, Menezes C, et al. The vesicular acetylcholine transporter is required for neuromuscular development and function. Mol Cell Biol. 2009;29:5238-50 pubmed publisher
    ..The abnormalities are similar to those of mice that cannot synthesize ACh due to a lack of choline acetyltransferase. Our results indicate that VAChT is essential to the normal development of motor neurons and the release of ACh. ..
  22. Paolone G, Mallory C, Cherian A, Miller T, Blakely R, Sarter M. Monitoring cholinergic activity during attentional performance in mice heterozygous for the choline transporter: a model of cholinergic capacity limits. Neuropharmacology. 2013;75:274-85 pubmed publisher
    Reductions in the capacity of the human choline transporter (SLC5A7, CHT) have been hypothesized to diminish cortical cholinergic neurotransmission, leading to risk for cognitive and mood disorders...