Slc26a3

Summary

Gene Symbol: Slc26a3
Description: solute carrier family 26, member 3
Alias: 9030623B18Rik, 9130013M11Rik, AV376035, Dra, chloride anion exchanger, down-regulated in adenoma
Species: mouse
Products:     Slc26a3

Top Publications

  1. Kumar A, Chatterjee I, Gujral T, Alakkam A, Coffing H, Anbazhagan A, et al. Activation of Nuclear Factor-κB by Tumor Necrosis Factor in Intestinal Epithelial Cells and Mouse Intestinal Epithelia Reduces Expression of the Chloride Transporter SLC26A3. Gastroenterology. 2017;153:1338-1350.e3 pubmed publisher
    ..mucosa of patients with inflammatory bowel diseases has reduced expression of solute carrier family 26 member 3 (SLC26A3, also called DRA)...
  2. Whittamore J, Freel R, Hatch M. Sulfate secretion and chloride absorption are mediated by the anion exchanger DRA (Slc26a3) in the mouse cecum. Am J Physiol Gastrointest Liver Physiol. 2013;305:G172-84 pubmed publisher
    ..A leading candidate is the apical chloride/bicarbonate (Cl?/HCO??) exchanger DRA (down-regulated in adenoma; Slc26a3), primarily linked to the Cl? transporting defect in congenital chloride diarrhea...
  3. TALBOT C, Lytle C. Segregation of Na/H exchanger-3 and Cl/HCO3 exchanger SLC26A3 (DRA) in rodent cecum and colon. Am J Physiol Gastrointest Liver Physiol. 2010;299:G358-67 pubmed publisher
    ..is believed to absorb NaCl via the coupled operation of apical Na/H exchanger-3 (NHE3) and Cl/HCO(3) exchanger SLC26A3 (DRA)...
  4. Simpson J, Schweinfest C, Shull G, Gawenis L, Walker N, Boyle K, et al. PAT-1 (Slc26a6) is the predominant apical membrane Cl-/HCO3- exchanger in the upper villous epithelium of the murine duodenum. Am J Physiol Gastrointest Liver Physiol. 2007;292:G1079-88 pubmed
    ..deletion of the following Cl(-)/HCO(3)(-) exchangers localized to the apical membrane of murine duodenal villi: Slc26a3 [down-regulated in adenoma (DRA)], Slc26a6 [putative anion transporter 1 (PAT-1)], and Slc4a9 [anion exchanger 4 (..
  5. Walker N, Simpson J, Yen P, Gill R, Rigsby E, Brazill J, et al. Down-regulated in adenoma Cl/HCO3 exchanger couples with Na/H exchanger 3 for NaCl absorption in murine small intestine. Gastroenterology. 2008;135:1645-1653.e3 pubmed publisher
    ..exchanger(s) was investigated using mice with gene-targeted knockout (KO) of Cl(-)/HCO(3)(-) exchangers: Slc26a3, down-regulated in adenoma (Dra) or Slc26a6, putative anion transporter-1 (Pat-1)...
  6. Bhutia Y, Babu E, Ramachandran S, Yang S, Thangaraju M, Ganapathy V. SLC transporters as a novel class of tumour suppressors: identity, function and molecular mechanisms. Biochem J. 2016;473:1113-24 pubmed publisher
    ..It also links colonic bacteria and dietary fibre to the host. SLC26A3 as a tumour suppressor is restricted to colon; it is a Cl(-)/HCO(-) 3 exchanger, facilitating the efflux of HCO(-)..
  7. Xia W, Yu Q, Riederer B, Singh A, Engelhardt R, Yeruva S, et al. The distinct roles of anion transporters Slc26a3 (DRA) and Slc26a6 (PAT-1) in fluid and electrolyte absorption in the murine small intestine. Pflugers Arch. 2014;466:1541-56 pubmed
    ..We investigated the role of the small intestinal apical anion exchangers PAT-1 (Slc26a6) and DRA (Slc26a3) in basal and CO2/HCO3 ?-stimulated jejunal fluid absorption...
  8. Xiao F, Jurić M, Li J, Riederer B, Yeruva S, Singh A, et al. Loss of downregulated in adenoma (DRA) impairs mucosal HCO3(-) secretion in murine ileocolonic inflammation. Inflamm Bowel Dis. 2012;18:101-11 pubmed publisher
    ..Downregulated in adenoma (DRA) mRNA and protein expression was strongly decreased in TNF(+/?ARE) ileocolon, whereas cystic fibrosis transmembrane ..
  9. Raheja G, Singh V, Ma K, Boumendjel R, Borthakur A, Gill R, et al. Lactobacillus acidophilus stimulates the expression of SLC26A3 via a transcriptional mechanism. Am J Physiol Gastrointest Liver Physiol. 2010;298:G395-401 pubmed publisher
    ..stimulated Cl(-)/OH(-) exchange activity via an increase in the surface levels of the apical anion exchanger SLC26A3 (DRA). However, whether probiotics influence SLC26A3 expression and promoter activity has not been examined...

More Information

Publications36

  1. Schweinfest C, Spyropoulos D, Henderson K, Kim J, Chapman J, Barone S, et al. slc26a3 (dra)-deficient mice display chloride-losing diarrhea, enhanced colonic proliferation, and distinct up-regulation of ion transporters in the colon. J Biol Chem. 2006;281:37962-71 pubmed
    Mutations in the SLC26A3 (DRA (down-regulated in adenoma)) gene constitute the molecular etiology of congenital chloride-losing diarrhea in humans...
  2. Alper S, Stewart A, Vandorpe D, Clark J, Horack R, Simpson J, et al. Native and recombinant Slc26a3 (downregulated in adenoma, Dra) do not exhibit properties of 2Cl-/1HCO3- exchange. Am J Physiol Cell Physiol. 2011;300:C276-86 pubmed publisher
    The recent proposal that Dra/Slc26a3 mediates electrogenic 2Cl(-)/1HCO(3)(-) exchange suggests a required revision of classical concepts of electroneutral Cl(-) transport across epithelia such as the intestine...
  3. Ishiguro H, Namkung W, Yamamoto A, Wang Z, Worrell R, Xu J, et al. Effect of Slc26a6 deletion on apical Cl-/HCO3- exchanger activity and cAMP-stimulated bicarbonate secretion in pancreatic duct. Am J Physiol Gastrointest Liver Physiol. 2007;292:G447-55 pubmed
    ..Semiquantitative RT-PCR demonstrated more than fivefold upregulation in Slc26a3 (DRA) expression in Slc26a6 knockout pancreas...
  4. Freel R, Whittamore J, Hatch M. Transcellular oxalate and Cl- absorption in mouse intestine is mediated by the DRA anion exchanger Slc26a3, and DRA deletion decreases urinary oxalate. Am J Physiol Gastrointest Liver Physiol. 2013;305:G520-7 pubmed publisher
    ..Apical exchangers of the Slc26a family that mediate oxalate absorption have not been established, yet the Slc26a3 [downregulated in adenoma (DRA)] protein is a candidate mediator of oxalate uptake...
  5. Singh A, Liu Y, Riederer B, Engelhardt R, Thakur B, Soleimani M, et al. Molecular transport machinery involved in orchestrating luminal acid-induced duodenal bicarbonate secretion in vivo. J Physiol. 2013;591:5377-91 pubmed publisher
    ..membrane expresses several ion transporters and/or channels, including the solute carrier 26 anion transporters Slc26a3 (DRA) and Slc26a6 (PAT-1), the Na(+)/H(+) exchanger isoform 3 (NHE3), as well as the anion channels cystic ..
  6. Jacob P, Rossmann H, Lamprecht G, Kretz A, Neff C, Lin Wu E, et al. Down-regulated in adenoma mediates apical Cl-/HCO3- exchange in rabbit, rat, and human duodenum. Gastroenterology. 2002;122:709-24 pubmed
    ..The recently discovered dra (down-regulated in adenoma) gene encodes a transport protein (DRA) for SO4(2-), Cl-, and HCO3-...
  7. Chen L, Xu W. [Expression pattern of congenital chloride diarrhea pathogenic gene Slc26a3 in the reproductive tract of male rodents]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2014;31:654-8 pubmed publisher
    To determine the expression pattern of Slc26a3 gene in reproductive tract of male rodents to clarify whether the expression pattern is related to the subfertility observed in congenital chloride diarrhea (CLD) disease...
  8. Ohana E, Shcheynikov N, Yang D, So I, Muallem S. Determinants of coupled transport and uncoupled current by the electrogenic SLC26 transporters. J Gen Physiol. 2011;137:239-51 pubmed publisher
    ..A unique feature of the two members of the family, Slc26a3 and Slc26a6, is that they can function as both obligate coupled and mediate an uncoupled current, in a channel-..
  9. Kunieda T, Takahashi K, Shinkai Y, Tsuji T, Schweinfest C, Katoh H. The down-regulated in adenoma (Dra) gene encoding intestine-specific anion transporter maps to mouse chromosome 12. Exp Anim. 2000;49:67-8 pubmed
  10. Zhao F, Franco H, Rodriguez K, Brown P, Tsai M, Tsai S, et al. Elimination of the male reproductive tract in the female embryo is promoted by COUP-TFII in mice. Science. 2017;357:717-720 pubmed publisher
    ..We thus suggest that elimination of Wolffian ducts in female embryos is actively promoted by COUP-TFII, which suppresses a mesenchyme-epithelium cross-talk responsible for Wolffian duct maintenance. ..
  11. Borenshtein D, Schlieper K, Rickman B, Chapman J, Schweinfest C, Fox J, et al. Decreased expression of colonic Slc26a3 and carbonic anhydrase iv as a cause of fatal infectious diarrhea in mice. Infect Immun. 2009;77:3639-50 pubmed publisher
    ..strains C3H/HeOu and FVB/N was associated with significant fluid loss in feces, a remarkable downregulation of Slc26a3 and carbonic anhydrase IV (CAIV) message and protein expression, retention of chloride in stool, and ..
  12. Kratz C, Emerling B, Donovan S, Laig Webster M, Taylor B, Thompson P, et al. Candidate gene isolation and comparative analysis of a commonly deleted segment of 7q22 implicated in myeloid malignancies. Genomics. 2001;77:171-80 pubmed
    ..These resources should greatly accelerate the pace of candidate gene discovery in this region. ..
  13. Chavez J, Hernández González E, Wertheimer E, Visconti P, Darszon A, Trevino C. Participation of the Cl-/HCO(3)- exchangers SLC26A3 and SLC26A6, the Cl- channel CFTR, and the regulatory factor SLC9A3R1 in mouse sperm capacitation. Biol Reprod. 2012;86:1-14 pubmed publisher
    ..Alternatively, the [Cl(-)](i) increase might be mediated by anion exchangers. Among them, SLC26A3 and SLC26A6 are good candidates, since, in several cell types, they increase [Cl(-)](i) and interact with cystic ..
  14. Singh V, Kumar A, Raheja G, Anbazhagan A, Priyamvada S, Saksena S, et al. Lactobacillus acidophilus attenuates downregulation of DRA function and expression in inflammatory models. Am J Physiol Gastrointest Liver Physiol. 2014;307:G623-31 pubmed publisher
    ..Cl(-)/HCO3 (-) exchange activity, acutely via an increase in the surface levels of downregulated in adenoma (DRA, SLC26A3) and in long-term treatments via increasing its expression involving transcriptional mechanisms...
  15. Xiao F, Yu Q, Li J, Johansson M, Singh A, Xia W, et al. Slc26a3 deficiency is associated with loss of colonic HCO3 (-) secretion, absence of a firm mucus layer and barrier impairment in mice. Acta Physiol (Oxf). 2014;211:161-75 pubmed publisher
    Downregulated in adenoma (DRA, Slc26a3) is a member of the solute carrier family 26 (SLC26), family of anion transporters, which is mutated in familial chloride-losing diarrhoea (CLD)...
  16. Dawson P, Rakoczy J, Simmons D. Placental, renal, and ileal sulfate transporter gene expression in mouse gestation. Biol Reprod. 2012;87:43 pubmed publisher
    ..sulfate transporter mRNA levels were not increased in gestation, but slight decreases (by ?30-40%) were found for Slc26a3 and Slc26a6. In placentae, Slc13a4 and Slc26a2 mRNAs were most abundant, with levels increasing from E10...
  17. Melvin J, Park K, Richardson L, Schultheis P, Shull G. Mouse down-regulated in adenoma (DRA) is an intestinal Cl(-)/HCO(3)(-) exchanger and is up-regulated in colon of mice lacking the NHE3 Na(+)/H(+) exchanger. J Biol Chem. 1999;274:22855-61 pubmed
    Mutations in human DRA cause congenital chloride diarrhea, thereby raising the possibility that it functions as a Cl(-)/HCO(3)(-) exchanger...
  18. Singh A, Riederer B, Chen M, Xiao F, Krabbenhöft A, Engelhardt R, et al. The switch of intestinal Slc26 exchangers from anion absorptive to HCOFormula secretory mode is dependent on CFTR anion channel function. Am J Physiol Cell Physiol. 2010;298:C1057-65 pubmed publisher
    ..Slc26a6 (PAT1) or Slc26a3 (DRA) ablation reduced the S1611-induced DBS increase and reduced fluid absorptive rates, suggesting that the ..
  19. Walker N, Simpson J, Brazill J, Gill R, Dudeja P, Schweinfest C, et al. Role of down-regulated in adenoma anion exchanger in HCO3- secretion across murine duodenum. Gastroenterology. 2009;136:893-901 pubmed publisher
    ..Therefore, the role of down-regulated in adenoma (Dra) in duodenal HCO(3)(-) secretion was investigated using DraKO mice...
  20. Song Y, Yamamoto A, Steward M, Ko S, Stewart A, Soleimani M, et al. Deletion of Slc26a6 alters the stoichiometry of apical Cl-/HCO-3 exchange in mouse pancreatic duct. Am J Physiol Cell Physiol. 2012;303:C815-24 pubmed publisher
    ..7 in wild-type ducts and ?1.4 in Slc26a6(-/-) ducts where a different Cl(-)/HCO(3)(-) exchanger, most likely SLC26A3, was found to be active...
  21. Silberg D, Wang W, Moseley R, Traber P. The Down regulated in Adenoma (dra) gene encodes an intestine-specific membrane sulfate transport protein. J Biol Chem. 1995;270:11897-902 pubmed
    A gene has been described, Down Regulated in Adenoma (dra), which is expressed in normal colon but is absent in the majority of colon adenomas and adenocarcinomas. However, the function of this protein is unknown...
  22. Jalali R, Zandieh Doulabi B, DenBesten P, Seidler U, Riederer B, Wedenoja S, et al. Slc26a3/Dra and Slc26a6 in Murine Ameloblasts. J Dent Res. 2015;94:1732-9 pubmed publisher
    ..Solute carrier (Slc) family 26A encodes different anion exchangers that exchange Cl(-)/HCO3 (-), including Slc26a3/Dra, Slc26a6/Pat-1, and Slc26a4/pendrin...
  23. Anbazhagan A, Priyamvada S, Kumar A, Maher D, Borthakur A, Alrefai W, et al. Translational repression of SLC26A3 by miR-494 in intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2014;306:G123-31 pubmed publisher
    b>SLC26A3 [downregulated in adenoma (DRA)] is a Cl(-)/HCO3(-) exchanger involved in electroneutral NaCl absorption in the mammalian intestine. Altered DRA expression levels are associated with infectious and inflammatory diarrheal diseases...
  24. Kumar A, Hecht C, Priyamvada S, Anbazhagan A, Alakkam A, Borthakur A, et al. Probiotic Bifidobacterium species stimulate human SLC26A3 gene function and expression in intestinal epithelial cells. Am J Physiol Cell Physiol. 2014;307:C1084-92 pubmed publisher
    b>SLC26A3, or downregulated in adenoma (DRA), plays a major role in mediating Cl(-) absorption in the mammalian intestine...
  25. D Angelo A, Bluteau O, Garcia Gonzalez M, Gresh L, Doyen A, Garbay S, et al. Hepatocyte nuclear factor 1alpha and beta control terminal differentiation and cell fate commitment in the gut epithelium. Development. 2010;137:1573-82 pubmed publisher
    ..with a defect in intestinal water absorption and that Hnf1alpha and beta directly control the expression of Slc26a3, a gene whose mutations are associated with chloride diarrhoea in human patients...
  26. Gujral T, Kumar A, Priyamvada S, Saksena S, Gill R, Hodges K, et al. Mechanisms of DRA recycling in intestinal epithelial cells: effect of enteropathogenic E. coli. Am J Physiol Cell Physiol. 2015;309:C835-46 pubmed publisher
    ..EPEC decreases the activity and surface expression of the key intestinal Cl(-)/HCO3(-) exchanger SLC26A3 [downregulated in adenoma (DRA)], contributing to the pathophysiology of early diarrhea...
  27. Whittamore J, Hatch M. Loss of the anion exchanger DRA (Slc26a3), or PAT1 (Slc26a6), alters sulfate transport by the distal ileum and overall sulfate homeostasis. Am J Physiol Gastrointest Liver Physiol. 2017;313:G166-G179 pubmed publisher
    ..of the apical chloride/bicarbonate (Cl-/[Formula: see text]) exchangers downregulated in adenoma (DRA; Slc26a3), and putative anion transporter 1 (PAT1; Slc26a6), to the underlying transport mechanism...