Gene Symbol: Shroom3
Description: shroom family member 3
Alias: AL022960, D5Ertd287e, Shrm, Shrm3, protein Shroom3
Species: mouse
Products:     Shroom3

Top Publications

  1. Taylor J, Chung K, Figueroa C, Zurawski J, Dickson H, Brace E, et al. The scaffold protein POSH regulates axon outgrowth. Mol Biol Cell. 2008;19:5181-92 pubmed publisher
    ..a requirement for the third Src homology (SH) 3 domain of POSH, and we find that the actomyosin regulatory protein Shroom3 interacts with this domain of POSH...
  2. Hildebrand J, Soriano P. Shroom, a PDZ domain-containing actin-binding protein, is required for neural tube morphogenesis in mice. Cell. 1999;99:485-97 pubmed
    ..This mutation is designated shroom (shrm) because the neural folds "mushroom" outward and do not converge at the dorsal midline...
  3. Grosse A, Pressprich M, Curley L, Hamilton K, Margolis B, Hildebrand J, et al. Cell dynamics in fetal intestinal epithelium: implications for intestinal growth and morphogenesis. Development. 2011;138:4423-32 pubmed publisher
    ..Finally, we show that the actin-binding protein Shroom3 is crucial for the maintenance of the single-layered pseudostratified epithelium...
  4. Plageman T, Zacharias A, Gage P, Lang R. Shroom3 and a Pitx2-N-cadherin pathway function cooperatively to generate asymmetric cell shape changes during gut morphogenesis. Dev Biol. 2011;357:227-34 pubmed publisher
    The cytoskeletal protein Shroom3 is a potent inducer of epithelial cell shape change and is required for lens and neural plate morphogenesis...
  5. Marean A, Graf A, Zhang Y, Niswander L. Folic acid supplementation can adversely affect murine neural tube closure and embryonic survival. Hum Mol Genet. 2011;20:3678-83 pubmed publisher
    ..the relationship between genetics and responsiveness to FA supplementation, we used five mouse NTDs models-Zic2, Shroom3, Frem2, Grhl2 (Grainyhead-like 2) and L3P (Line3P)-and a long-term generational FA supplementation scheme...
  6. Plageman T, Chung M, Lou M, Smith A, Hildebrand J, Wallingford J, et al. Pax6-dependent Shroom3 expression regulates apical constriction during lens placode invagination. Development. 2010;137:405-15 pubmed publisher
    ..apical constriction of lens epithelial cells that accompanies invagination of the lens placode is dependent on Shroom3, a molecule previously associated with apical constriction during morphogenesis of the neural plate...
  7. Nishimura T, Takeichi M. Shroom3-mediated recruitment of Rho kinases to the apical cell junctions regulates epithelial and neuroepithelial planar remodeling. Development. 2008;135:1493-502 pubmed publisher
    Remodeling of epithelial sheets plays important roles in animal morphogenesis. Shroom3 is known to regulate the apical constriction of epithelial cells...
  8. Hildebrand J. Shroom regulates epithelial cell shape via the apical positioning of an actomyosin network. J Cell Sci. 2005;118:5191-203 pubmed
    ..Thus, Shroom likely facilitates neural tube closure by regulating cell shape changes via the apical positioning of an actomyosin network in the neurepithelium. ..
  9. Mohan S, Rizaldy R, Das D, Bauer R, Heroux A, Trakselis M, et al. Structure of Shroom domain 2 reveals a three-segmented coiled-coil required for dimerization, Rock binding, and apical constriction. Mol Biol Cell. 2012;23:2131-42 pubmed publisher
    Shroom (Shrm) proteins are essential regulators of cell shape and tissue morpho-logy during animal development that function by interacting directly with the coiled-coil region of Rho kinase (Rock)...

More Information


  1. Massarwa R, Niswander L. In toto live imaging of mouse morphogenesis and new insights into neural tube closure. Development. 2013;140:226-36 pubmed publisher
    ..We demonstrate the advantages of this imaging system by following the dynamics of neural tube closure during mouse embryogenesis and reveal extensive movements of the cranial neural tissue that are independent of neural fold zipping. ..
  2. Lang R, Herman K, Reynolds A, Hildebrand J, Plageman T. p120-catenin-dependent junctional recruitment of Shroom3 is required for apical constriction during lens pit morphogenesis. Development. 2014;141:3177-87 pubmed publisher
    ..In addition we found that, among several junctional components, p120-catenin genetically interacts with Shroom3, a protein required for AC during embryonic morphogenesis...
  3. Etournay R, Zwaenepoel I, Perfettini I, Legrain P, Petit C, El Amraoui A. Shroom2, a myosin-VIIa- and actin-binding protein, directly interacts with ZO-1 at tight junctions. J Cell Sci. 2007;120:2838-50 pubmed
  4. Dickson H, Zurawski J, Zhang H, Turner D, Vojtek A. POSH is an intracellular signal transducer for the axon outgrowth inhibitor Nogo66. J Neurosci. 2010;30:13319-25 pubmed publisher
    ..Blocking the function of POSH, or two POSH-associated proteins, leucine zipper kinase (LZK) and Shroom3, with RNAi in cortical neurons leads to release from myelin and Nogo66 inhibition...
  5. Seedorf H, Springer I, Grundner Culemann E, Albers H, Reis A, Fuchs H, et al. Amelogenesis imperfecta in a new animal model--a mutation in chromosome 5 (human 4q21). J Dent Res. 2004;83:608-12 pubmed
    ..We were able to demonstrate that a mutation on chromosome 5 corresponding to human chromosome 4q21 can cause amelogenesis imperfecta and changes in dentin composition. ..
  6. Klezovitch O, Fernandez T, Tapscott S, Vasioukhin V. Loss of cell polarity causes severe brain dysplasia in Lgl1 knockout mice. Genes Dev. 2004;18:559-71 pubmed
  7. Muccioli M, Qaisi D, Herman K, Plageman T. Lens placode planar cell polarity is dependent on Cdc42-mediated junctional contraction inhibition. Dev Biol. 2016;412:32-43 pubmed publisher
    ..localization of actomyosin and their contraction is dependent on the F-actin and Rho-kinase binding protein, Shroom3. Elongation of circumferential junctions is dependent upon Cdc42, a Rho-GTPase known to regulate polarity via the ..
  8. Davidson C, Li Q, Churchill G, Osborne L, McDermid H. Modifier locus for exencephaly in Cecr2 mutant mice is syntenic to the 10q25.3 region associated with neural tube defects in humans. Physiol Genomics. 2007;31:244-51 pubmed
  9. Esterbauer H, Oberkofler H, Krempler F, Patsch W. Human peroxisome proliferator activated receptor gamma coactivator 1 (PPARGC1) gene: cDNA sequence, genomic organization, chromosomal localization, and tissue expression. Genomics. 1999;62:98-102 pubmed
    ..Hence, PPARGC1 expression might influence insulin sensitivity as well as energy expenditure, thereby contributing to the development and pathophysiology of human obesity. ..
  10. Chu C, Gerstenzang E, Ossipova O, Sokol S. Lulu regulates Shroom-induced apical constriction during neural tube closure. PLoS ONE. 2013;8:e81854 pubmed publisher
    ..Depletion of endogenous Lulu disrupted the localization and activity of Shroom3, a PDZ-containing actin-binding protein that has also been implicated in apical constriction...
  11. Das D, Zalewski J, Mohan S, Plageman T, VanDemark A, Hildebrand J. The interaction between Shroom3 and Rho-kinase is required for neural tube morphogenesis in mice. Biol Open. 2014;3:850-60 pubmed publisher
    b>Shroom3 is an actin-associated regulator of cell morphology that is required for neural tube closure, formation of the lens placode, and gut morphogenesis in mice and has been linked to chronic kidney disease and directional heart ..
  12. McGreevy E, Vijayraghavan D, Davidson L, Hildebrand J. Shroom3 functions downstream of planar cell polarity to regulate myosin II distribution and cellular organization during neural tube closure. Biol Open. 2015;4:186-96 pubmed publisher
    ..Here we investigate the role of Shroom3 as a direct linker between PCP and actomyosin contractility during mouse neural tube morphogenesis...
  13. Loebel D, Plageman T, Tang T, Jones V, Muccioli M, Tam P. Thyroid bud morphogenesis requires CDC42- and SHROOM3-dependent apical constriction. Biol Open. 2016;5:130-9 pubmed publisher
    ..b>SHROOM3, a protein that interacts with Rho GTPases and promotes apical constriction, was strongly expressed in the ..