Runx2

Summary

Gene Symbol: Runx2
Description: runt related transcription factor 2
Alias: AML3, Cbf, Cbfa-1, Cbfa1, LS3, Osf2, PEBP2aA, Pebp2a1, Pebpa2a, runt-related transcription factor 2, AKV core binding factor, CBF-alpha-1, PEA2-alpha A, PEBP2 alpha A, SL3-3 enhancer factor 1 alpha A subunit, acute myeloid leukemia 3 protein, core binding factor alpha 1, osteoblast-specific transcription factor 2, polyomavirus enhancer-binding protein 2 alpha A subunit, runt domain, alpha subunit 1
Species: mouse
Products:     Runx2

Top Publications

  1. Lou Y, Javed A, Hussain S, Colby J, Frederick D, Pratap J, et al. A Runx2 threshold for the cleidocranial dysplasia phenotype. Hum Mol Genet. 2009;18:556-68 pubmed publisher
    ..autosomal-dominant skeletal disease that results from mutations in the bone-specific transcription factor RUNX2 (CBFA1/AML3). However, distinct RUNX2 mutations in CCD do not correlate with the severity of the disease...
  2. Tamiya H, Ikeda T, Jeong J, Saito T, Yano F, Jung Y, et al. Analysis of the Runx2 promoter in osseous and non-osseous cells and identification of HIF2A as a potent transcription activator. Gene. 2008;416:53-60 pubmed publisher
    Little is known about the upstream regulator of Runx2, a master regulator of osteoblast differentiation in bone tissues...
  3. Vitolo M, Anglin I, Mahoney W, Renoud K, Gartenhaus R, Bachman K, et al. The RUNX2 transcription factor cooperates with the YES-associated protein, YAP65, to promote cell transformation. Cancer Biol Ther. 2007;6:856-63 pubmed
    ..Although RUNX2 is an essential bone maturation factor, it has also been found to promote transformation in vivo and cell ..
  4. Ting M, Wu N, Roybal P, Sun J, Liu L, Yen Y, et al. EphA4 as an effector of Twist1 in the guidance of osteogenic precursor cells during calvarial bone growth and in craniosynostosis. Development. 2009;136:855-64 pubmed publisher
    ..We suggest that the failure of this process in Twist1 and EphA4 mutants is the cause of craniosynostosis. ..
  5. Tao J, Chen S, Yang T, Dawson B, Munivez E, Bertin T, et al. Osteosclerosis owing to Notch gain of function is solely Rbpj-dependent. J Bone Miner Res. 2010;25:2175-83 pubmed publisher
    ..As such, it identifies Rbpj as a specific target for manipulating Notch signaling in a cell-autonomous fashion in osteoblasts in bone diseases where Notch may be dysregulated...
  6. Ding M, Lu Y, Abbassi S, Li F, Li X, Song Y, et al. Targeting Runx2 expression in hypertrophic chondrocytes impairs endochondral ossification during early skeletal development. J Cell Physiol. 2012;227:3446-56 pubmed publisher
    b>Runx2 is a known master transcription factor for osteoblast differentiation, as well as an essential regulator for chondrocyte maturation...
  7. Wu M, Hesse E, Morvan F, Zhang J, Correa D, Rowe G, et al. Zfp521 antagonizes Runx2, delays osteoblast differentiation in vitro, and promotes bone formation in vivo. Bone. 2009;44:528-36 pubmed publisher
    ..Zfp521 binds Runx2 and represses its transcriptional activity, and Runx2 dose-dependently rescues Zfp521's inhibition of osteoblast ..
  8. Kwon T, Zhao X, Yang Q, Li Y, Ge C, Zhao G, et al. Physical and functional interactions between Runx2 and HIF-1? induce vascular endothelial growth factor gene expression. J Cell Biochem. 2011;112:3582-93 pubmed publisher
    ..On the other hand, deficiency of Runx2, a key osteogenic transcription factor, prevents vascular invasion of bone and VEGF expression...
  9. Young D, Hassan M, Pratap J, Galindo M, Zaidi S, Lee S, et al. Mitotic occupancy and lineage-specific transcriptional control of rRNA genes by Runx2. Nature. 2007;445:442-6 pubmed
    ..Runt-related transcription factors Runx1, Runx2 and Runx3 establish and maintain cell identity, and convey phenotypic information through successive cell ..

More Information

Publications79

  1. Komori T. Regulation of bone development and extracellular matrix protein genes by RUNX2. Cell Tissue Res. 2010;339:189-95 pubmed publisher
    b>RUNX2 is a multifunctional transcription factor that controls skeletal development by regulating the differentiation of chondrocytes and osteoblasts and the expression of many extracellular matrix protein genes during chondrocyte and ..
  2. Sun Y, Byon C, Yuan K, Chen J, Mao X, Heath J, et al. Smooth muscle cell-specific runx2 deficiency inhibits vascular calcification. Circ Res. 2012;111:543-52 pubmed publisher
    ..We have previously reported that the osteogenic transcription factor Runx2 is an essential and sufficient regulator of calcification of vascular smooth muscle cells (VSMC) in vitro...
  3. Hattori T, Müller C, Gebhard S, Bauer E, Pausch F, Schlund B, et al. SOX9 is a major negative regulator of cartilage vascularization, bone marrow formation and endochondral ossification. Development. 2010;137:901-11 pubmed publisher
    ..These findings imply that downregulation of Sox9 in the hypertrophic zone of the normal growth plate is essential for allowing vascular invasion, bone marrow formation and endochondral ossification. ..
  4. Selvamurugan N, Shimizu E, Lee M, Liu T, Li H, Partridge N. Identification and characterization of Runx2 phosphorylation sites involved in matrix metalloproteinase-13 promoter activation. FEBS Lett. 2009;583:1141-6 pubmed publisher
    ..PTH acts via protein kinase A (PKA) to phosphorylate and stimulate the transactivation of Runx2 for MMP-13 promoter activation. We show here that PTH stimulated Runx2 phosphorylation in rat osteoblastic cells...
  5. Zhang C, Tang W, Li Y, Yang F, Dowd D, MacDonald P. Osteoblast-specific transcription factor Osterix increases vitamin D receptor gene expression in osteoblasts. PLoS ONE. 2011;6:e26504 pubmed publisher
    ..They further provide new insight into potential mechanisms and pathways that Osx controls in osteoblasts and during the process of osteoblastic cell differentiation. ..
  6. Zaidi S, Pande S, Pratap J, Gaur T, Grigoriu S, Ali S, et al. Runx2 deficiency and defective subnuclear targeting bypass senescence to promote immortalization and tumorigenic potential. Proc Natl Acad Sci U S A. 2007;104:19861-6 pubmed
    The osteogenic Runt-related (Runx2) transcription factor negatively regulates proliferation and ribosomal gene expression in normal diploid osteoblasts, but is up-regulated in metastatic breast and prostate cancer cells...
  7. Joeng K, Schumacher C, Zylstra Diegel C, Long F, Williams B. Lrp5 and Lrp6 redundantly control skeletal development in the mouse embryo. Dev Biol. 2011;359:222-9 pubmed publisher
    ..Thus, Lrp5 and 6 redundantly control embryonic skeletal development, likely through ?-catenin signaling. ..
  8. Roca H, Franceschi R. Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation. Nucleic Acids Res. 2008;36:1723-30 pubmed publisher
    ..Using this approach, we were able to discriminate between individual binding sites for the Runx2 transcription factor in the osteocalcin and bone sialoprotein genes that cannot be resolved by traditional X-ChIP...
  9. Takarada T, Yoneda Y. Transactivation by Runt related factor-2 of matrix metalloproteinase-13 in astrocytes. Neurosci Lett. 2009;451:99-104 pubmed publisher
    ..expression was invariably seen for the master regulator of osteoblastic differentiation Runt related factor-2 (Runx2), in addition to the partner protein core binding factor-beta and their targets such as osteopontin (OPN) and ..
  10. Hu R, Liu W, Li H, Yang L, Chen C, Xia Z, et al. A Runx2/miR-3960/miR-2861 regulatory feedback loop during mouse osteoblast differentiation. J Biol Chem. 2011;286:12328-39 pubmed publisher
    ..differentiation by targeting histone deacetylase 5, resulting in increased runt-related transcription factor 2 (Runx2) protein production...
  11. Oh J, Seo J, Yoon W, Cho J, Baek J, Ryoo H, et al. Suppression of Runx2 protein degradation by fibrous engineered matrix. Biomaterials. 2011;32:5826-36 pubmed publisher
    ..In this study, we demonstrate that fibre structure of an engineered matrix suppresses the degradation of Runx2, a master transcription factor that can turn on to osteoblast differentiation...
  12. Teplyuk N, Haupt L, Ling L, Dombrowski C, Mun F, Nathan S, et al. The osteogenic transcription factor Runx2 regulates components of the fibroblast growth factor/proteoglycan signaling axis in osteoblasts. J Cell Biochem. 2009;107:144-54 pubmed publisher
    ..FGF2 signaling modulates the expression and activity of Runt-related transcription factor 2 (Runx2/Cbfa1), a key regulator of osteoblast proliferation and maturation...
  13. Yu S, Geng Q, Sun F, Yu Y, Pan Q, Hong A. Osteogenic differentiation of C2C12 myogenic progenitor cells requires the Fos-related antigen Fra-1 - a novel target of Runx2. Biochem Biophys Res Commun. 2013;430:173-8 pubmed publisher
    ..It is established that Runx2 tightly regulates osteoblast differentiation and bone formation...
  14. Khalid O, Baniwal S, Purcell D, Leclerc N, Gabet Y, Stallcup M, et al. Modulation of Runx2 activity by estrogen receptor-alpha: implications for osteoporosis and breast cancer. Endocrinology. 2008;149:5984-95 pubmed publisher
    The transcription factors Runx2 and estrogen receptor-alpha (ERalpha) are involved in numerous normal and disease processes, including postmenopausal osteoporosis and breast cancer...
  15. Liao X, Zhang Z, Yuan K, Liu Y, Feng X, Cui R, et al. MiR-133a modulates osteogenic differentiation of vascular smooth muscle cells. Endocrinology. 2013;154:3344-52 pubmed publisher
    ..into osteoblast-like cells as evidenced by a decrease in alkaline phosphatase activity, osteocalcin secretion, Runx2 expression, and mineralized nodule formation...
  16. Jeong J, Jin J, Kim H, Kang S, Liu J, Lengner C, et al. Expression of Runx2 transcription factor in non-skeletal tissues, sperm and brain. J Cell Physiol. 2008;217:511-7 pubmed publisher
    b>Runx2 is a master transcription factor for chondrocyte and osteoblast differentiation and bone formation. However expression of Runx2 (by RT-PCR), has been reported in non-skeletal tissues such as breast, T cells and testis...
  17. Kimura A, Inose H, Yano F, Fujita K, Ikeda T, Sato S, et al. Runx1 and Runx2 cooperate during sternal morphogenesis. Development. 2010;137:1159-67 pubmed publisher
    Chondrocyte differentiation is strictly regulated by various transcription factors, including Runx2 and Runx3; however, the physiological role of Runx1 in chondrocyte differentiation remains unknown...
  18. Liu J, Lengner C, Gaur T, Lou Y, Hussain S, Jones M, et al. Runx2 protein expression utilizes the Runx2 P1 promoter to establish osteoprogenitor cell number for normal bone formation. J Biol Chem. 2011;286:30057-70 pubmed publisher
    The Runt-related transcription factor, Runx2, is essential for osteogenesis and is controlled by both distal (P1) and proximal (P2) promoters...
  19. Luan Y, Yu X, Xu K, Ding B, Yu J, Huang Y, et al. The retinoblastoma protein is an essential mediator of osteogenesis that links the p204 protein to the Cbfa1 transcription factor thereby increasing its activity. J Biol Chem. 2007;282:16860-70 pubmed
    ..the coordinated activity of numerous proteins including the transcription factor core-binding factor alpha1 (Cbfa1). Deregulation of Cbfa1 results in metabolic bone diseases including osteoporosis and osteopetrosis...
  20. Li X, Huang M, Zheng H, Wang Y, Ren F, Shang Y, et al. CHIP promotes Runx2 degradation and negatively regulates osteoblast differentiation. J Cell Biol. 2008;181:959-72 pubmed publisher
    b>Runx2, an essential transactivator for osteoblast differentiation, is tightly regulated at both the transcriptional and posttranslational levels...
  21. Galindo M, Kahler R, Teplyuk N, Stein J, Lian J, Stein G, et al. Cell cycle related modulations in Runx2 protein levels are independent of lymphocyte enhancer-binding factor 1 (Lef1) in proliferating osteoblasts. J Mol Histol. 2007;38:501-6 pubmed
    Runt-related transcription factor Runx2 regulates osteogenic phenotype commitment and attenuates osteoblast growth. Runx2 levels are cell cycle regulated and maximal in the G1 phase of proliferating osteoblasts and during quiescence...
  22. Jensen E, Niu L, Caretti G, Nicol S, Teplyuk N, Stein G, et al. p68 (Ddx5) interacts with Runx2 and regulates osteoblast differentiation. J Cell Biochem. 2008;103:1438-51 pubmed
    b>Runx2 is an essential transcription factor for osteoblast development from mesenchymal progenitors...
  23. Tanaka T, Sato H, Doi H, Yoshida C, Shimizu T, Matsui H, et al. Runx2 represses myocardin-mediated differentiation and facilitates osteogenic conversion of vascular smooth muscle cells. Mol Cell Biol. 2008;28:1147-60 pubmed
    ..Although Runx2, a key osteogenic transcription factor, is expressed in atherosclerotic plaques, the molecular mechanisms by which ..
  24. Chandler R, Chandler K, McFarland K, Mortlock D. Bmp2 transcription in osteoblast progenitors is regulated by a distant 3' enhancer located 156.3 kilobases from the promoter. Mol Cell Biol. 2007;27:2934-51 pubmed
  25. Kimura H, Akiyama H, Nakamura T, de Crombrugghe B. Tenascin-W inhibits proliferation and differentiation of preosteoblasts during endochondral bone formation. Biochem Biophys Res Commun. 2007;356:935-41 pubmed
    ..Furthermore, TN-W was detectable in perichondrium/periosteum of Runx2-null and Osterix-null embryos, indicating that TN-W is expressed in preosteoblasts...
  26. Shimoyama A, Wada M, Ikeda F, Hata K, Matsubara T, Nifuji A, et al. Ihh/Gli2 signaling promotes osteoblast differentiation by regulating Runx2 expression and function. Mol Biol Cell. 2007;18:2411-8 pubmed
    ..Ihh treatment or Gli2 overexpression also up-regulated the expression of Runx2, an essential transcription factor for osteoblastogenesis, and enhanced the transcriptional activity and ..
  27. Zhang Y, Xie R, Croce C, Stein J, Lian J, Van Wijnen A, et al. A program of microRNAs controls osteogenic lineage progression by targeting transcription factor Runx2. Proc Natl Acad Sci U S A. 2011;108:9863-8 pubmed publisher
    ..in osteoblasts and chondrocytes is stringently controlled by the cell-fate-determining transcription factor Runx2. In this study, we directly addressed whether microRNAs (miRNAs) can control the osteogenic activity of Runx2 and ..
  28. Coré N, Caubit X, Metchat A, Boned A, Djabali M, Fasano L. Tshz1 is required for axial skeleton, soft palate and middle ear development in mice. Dev Biol. 2007;308:407-20 pubmed
    ..Finally, we demonstrate that Tshz1 is required for the development of the soft palate...
  29. Zhu J, Shimizu E, Zhang X, Partridge N, Qin L. EGFR signaling suppresses osteoblast differentiation and inhibits expression of master osteoblastic transcription factors Runx2 and Osterix. J Cell Biochem. 2011;112:1749-60 pubmed publisher
    ..we demonstrated that EGFR signaling reduced the expression of two major osteoblastic transcription factors Runx2 (type II) and Osterix in osteoblast differentiating cells...
  30. Zheng L, Baek H, Karsenty G, Justice M. Filamin B represses chondrocyte hypertrophy in a Runx2/Smad3-dependent manner. J Cell Biol. 2007;178:121-8 pubmed
    ..This aberrant mineralization is due to ectopic chondrocyte hypertrophy similar to that seen in mice expressing Runx2 in chondrocytes...
  31. Sugito H, Shibukawa Y, Kinumatsu T, Yasuda T, Nagayama M, Yamada S, et al. Ihh signaling regulates mandibular symphysis development and growth. J Dent Res. 2011;90:625-31 pubmed publisher
    ..By embryonic days 16.5 to 18.5, Sox9-expressing chondrocytes formed within condensed Tgf?-1/Runx2-expressing mesenchymal cells at the prospective symphyseal joint site, and established a growth-plate-like ..
  32. Hilton M, Tu X, Wu X, Bai S, Zhao H, Kobayashi T, et al. Notch signaling maintains bone marrow mesenchymal progenitors by suppressing osteoblast differentiation. Nat Med. 2008;14:306-14 pubmed publisher
    ..Notch signaling seemed to inhibit osteoblast differentiation through Hes or Hey proteins, which diminished Runx2 transcriptional activity via physical interaction...
  33. Fischer A, Steidl C, Wagner T, Lang E, Jakob P, Friedl P, et al. Combined loss of Hey1 and HeyL causes congenital heart defects because of impaired epithelial to mesenchymal transition. Circ Res. 2007;100:856-63 pubmed
    ..Thus, the Hey gene family shows overlap in controlling Notch induced endocardial epithelial to mesenchymal transition, a process critical for valve and septum formation. ..
  34. Zhang X, Ting K, Bessette C, Culiat C, Sung S, Lee H, et al. Nell-1, a key functional mediator of Runx2, partially rescues calvarial defects in Runx2(+/-) mice. J Bone Miner Res. 2011;26:777-91 pubmed publisher
    Mesenchymal stem cell commitment to an osteoprogenitor lineage requires the activity of Runx2, a molecule implicated in the etiopathology of multiple congenital craniofacial anomalies...
  35. Nishikawa K, Nakashima T, Takeda S, Isogai M, Hamada M, Kimura A, et al. Maf promotes osteoblast differentiation in mice by mediating the age-related switch in mesenchymal cell differentiation. J Clin Invest. 2010;120:3455-65 pubmed publisher
    ..cell bifurcation into osteoblasts and adipocytes by cooperating with the osteogenic transcription factor Runx2 and inhibiting the expression of the adipogenic transcription factor Pparg...
  36. Ikegami D, Akiyama H, Suzuki A, Nakamura T, Nakano T, Yoshikawa H, et al. Sox9 sustains chondrocyte survival and hypertrophy in part through Pik3ca-Akt pathways. Development. 2011;138:1507-19 pubmed publisher
    ..Thus, continued expression of Sox9 in differentiated chondrocytes is essential for subsequent hypertrophy and sustains chondrocyte-specific survival mechanisms by binding to the Pik3ca promoter, inducing Akt phosphorylation. ..
  37. Govoni K, Linares G, Chen S, Pourteymoor S, Mohan S. T-box 3 negatively regulates osteoblast differentiation by inhibiting expression of osterix and runx2. J Cell Biochem. 2009;106:482-90 pubmed publisher
    ..In addition, Tbx3 overexpression abolished increased osterix and runx2 expression observed during normal osteoblast differentiation, but the change in Msx1 and Msx2 expression over time ..
  38. Sato S, Kimura A, Ozdemir J, Asou Y, Miyazaki M, Jinno T, et al. The distinct role of the Runx proteins in chondrocyte differentiation and intervertebral disc degeneration: findings in murine models and in human disease. Arthritis Rheum. 2008;58:2764-75 pubmed publisher
    b>Runx2 is a transcription factor that regulates chondrocyte differentiation...
  39. Tu Q, Zhang J, Paz J, Wade K, Yang P, Chen J. Haploinsufficiency of Runx2 results in bone formation decrease and different BSP expression pattern changes in two transgenic mouse models. J Cell Physiol. 2008;217:40-7 pubmed publisher
    b>Runx2 has been identified as "a master gene" for the differentiation of osteoblasts and Runx2-deficient mice has demonstrated a complete absence of mature osteoblast and ossification...
  40. Rath Deschner B, Daratsianos N, Dühr S, Girmann N, Winter J, Kroll F, et al. The significance of RUNX2 in postnatal development of the mandibular condyle. J Orofac Orthop. 2010;71:17-31 pubmed publisher
    b>RUNX2, in the Runt gene family, is one of the most important transcription factors in the development of the skeletal system...
  41. Maes C, Goossens S, Bartunkova S, Drogat B, Coenegrachts L, Stockmans I, et al. Increased skeletal VEGF enhances beta-catenin activity and results in excessively ossified bones. EMBO J. 2010;29:424-41 pubmed publisher
    ..Moreover, the finding that VEGF can modulate beta-catenin activity may have widespread physiological and clinical ramifications. ..
  42. Matsubara T, Kida K, Yamaguchi A, Hata K, Ichida F, Meguro H, et al. BMP2 regulates Osterix through Msx2 and Runx2 during osteoblast differentiation. J Biol Chem. 2008;283:29119-25 pubmed publisher
    ..Considering that Runx2 overexpression induces Osterix, these results suggest that Osterix functions as downstream of Runx2...
  43. Arnold M, Kim Y, Czubryt M, Phan D, McAnally J, Qi X, et al. MEF2C transcription factor controls chondrocyte hypertrophy and bone development. Dev Cell. 2007;12:377-89 pubmed
    ..These findings reveal unexpected commonalities in the mechanisms governing muscle, cardiovascular, and bone development with respect to their regulation by MEF2 and class II HDACs. ..
  44. Nishimura R, Wakabayashi M, Hata K, Matsubara T, Honma S, Wakisaka S, et al. Osterix regulates calcification and degradation of chondrogenic matrices through matrix metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2 during endochondral ossification. J Biol Chem. 2012;287:33179-90 pubmed
    ..ossification is temporally and spatially regulated by several critical transcription factors, including Sox9, Runx2, and Runx3. Although the molecular mechanisms that control the late stages of endochondral ossification (e.g...
  45. Shibata S, Yokohama Tamaki T. An in situ hybridization study of Runx2, Osterix, and Sox9 in the anlagen of mouse mandibular condylar cartilage in the early stages of embryogenesis. J Anat. 2008;213:274-83 pubmed publisher
    ..We previously demonstrated that three transcription factors related to bone and cartilage formation, namely Runx2, Osterix and Sox9, are simultaneously expressed in the anlage of mandibular condylar cartilage (condylar anlage) ..
  46. Javed A, Afzal F, Bae J, Gutierrez S, Zaidi K, Pratap J, et al. Specific residues of RUNX2 are obligatory for formation of BMP2-induced RUNX2-SMAD complex to promote osteoblast differentiation. Cells Tissues Organs. 2009;189:133-7 pubmed publisher
    BMP2 signaling and RUNX2 regulatory pathways converge for transcriptional control of bone formation in vivo...
  47. Watanabe T, Nakano N, Muraoka R, Shimizu T, Okafuji N, Kurihara S, et al. Role of Msx2 as a promoting factor for Runx2 at the periodontal tension sides elicited by mechanical stress. Eur J Med Res. 2008;13:425-31 pubmed
    Early changes of Runx2 and Msx2 expressions were examined by immunohistochemistry in mouse periodontal ligament exposed to mechanical stress. 8-week-old ddY mouse was used as experimental animal...
  48. Javed A, Bae J, Afzal F, Gutierrez S, Pratap J, Zaidi S, et al. Structural coupling of Smad and Runx2 for execution of the BMP2 osteogenic signal. J Biol Chem. 2008;283:8412-22 pubmed publisher
    ..pathways, bone morphogenetic protein (BMP)/transforming growth factor-beta (TGFbeta) and the transcription factor RUNX2, are required for bone formation in vivo...
  49. Hesse E, Saito H, Kiviranta R, Correa D, Yamana K, Neff L, et al. Zfp521 controls bone mass by HDAC3-dependent attenuation of Runx2 activity. J Cell Biol. 2010;191:1271-83 pubmed publisher
    b>Runx2 is indispensable for osteoblast lineage commitment and early differentiation but also blocks osteoblast maturation, thereby causing bone loss in Runx2 transgenic mice. Zinc finger protein 521 (Zfp521) antagonizes Runx2 in vivo...
  50. Hassan M, Gordon J, Beloti M, Croce C, Van Wijnen A, Stein J, et al. A network connecting Runx2, SATB2, and the miR-23a~27a~24-2 cluster regulates the osteoblast differentiation program. Proc Natl Acad Sci U S A. 2010;107:19879-84 pubmed publisher
    ..How expression of bone-related miRs is regulated remains a compelling question. Here we report that Runx2, a transcription factor essential for osteoblastogenesis, negatively regulates expression of the miR cluster 23a?..
  51. Joeng K, Long F. The Gli2 transcriptional activator is a crucial effector for Ihh signaling in osteoblast development and cartilage vascularization. Development. 2009;136:4177-85 pubmed publisher
    ..These results therefore provide direct genetic evidence that Gli2 and Gli3 collectively mediate all major aspects of Ihh function during endochondral skeletal development. ..
  52. Teplyuk N, Galindo M, Teplyuk V, Pratap J, Young D, Lapointe D, et al. Runx2 regulates G protein-coupled signaling pathways to control growth of osteoblast progenitors. J Biol Chem. 2008;283:27585-97 pubmed publisher
    Runt-related transcription factor 2 (Runx2) controls lineage commitment, proliferation, and anabolic functions of osteoblasts as the subnuclear effector of multiple signaling axes (e.g...
  53. Leong D, Lim J, Goh X, Pratap J, Pereira B, Kwok H, et al. Cancer-related ectopic expression of the bone-related transcription factor RUNX2 in non-osseous metastatic tumor cells is linked to cell proliferation and motility. Breast Cancer Res. 2010;12:R89 pubmed publisher
    Metastatic breast cancer cells frequently and ectopically express the transcription factor RUNX2, which normally attenuates proliferation and promotes maturation of osteoblasts...
  54. Wang C, Yang S, Wang Z, Tan J, Xing S, Chen D, et al. PCAF acetylates Runx2 and promotes osteoblast differentiation. J Bone Miner Metab. 2013;31:381-9 pubmed publisher
    ..Runt-related gene 2 (Runx2) is a master transcriptional factor for osteoblast differentiation...
  55. Holmes G, Rothschild G, Roy U, Deng C, Mansukhani A, Basilico C. Early onset of craniosynostosis in an Apert mouse model reveals critical features of this pathology. Dev Biol. 2009;328:273-84 pubmed publisher
  56. Wang Y, Sun M, Uhlhorn V, Zhou X, Peter I, Martinez Abadias N, et al. Activation of p38 MAPK pathway in the skull abnormalities of Apert syndrome Fgfr2(+P253R) mice. BMC Dev Biol. 2010;10:22 pubmed publisher
    ..We previously reported an inbred transgenic mouse model with the Fgfr2 +/S252W mutation on the C57BL/6J background for Apert syndrome. Here we present a mouse model for the Fgfr2+/P253R mutation...
  57. Yu L, Liu H, Yan M, Yang J, Long F, Muneoka K, et al. Shox2 is required for chondrocyte proliferation and maturation in proximal limb skeleton. Dev Biol. 2007;306:549-59 pubmed
    ..A dramatic down-regulation of Runx2 and Runx3 could account for the lack of chondrocyte hypertrophy, while a down-regulation of Ihh expression may be ..
  58. Sierra O, Towler D. Runx2 trans-activation mediated by the MSX2-interacting nuclear target requires homeodomain interacting protein kinase-3. Mol Endocrinol. 2010;24:1478-97 pubmed publisher
    Runt-related transcription factor 2 (Runx2) and muscle segment homeobox homolog 2-interacting nuclear target (MINT) (Spen homolog) are transcriptional regulators critical for mammalian development...
  59. Saito T, Fukai A, Mabuchi A, Ikeda T, Yano F, Ohba S, et al. Transcriptional regulation of endochondral ossification by HIF-2alpha during skeletal growth and osteoarthritis development. Nat Med. 2010;16:678-86 pubmed publisher
    ..Hence, HIF-2alpha is a central transactivator that targets several crucial genes for endochondral ossification and may represent a therapeutic target for osteoarthritis. ..
  60. Deepak V, Zhang Z, Meng L, Zeng X, Liu W. Reduced activity and cytoplasmic localization of Runx2 is observed in C3h10t1/2 cells overexpressing Tbx3. Cell Biochem Funct. 2011;29:348-50 pubmed publisher
    ..Here, we studied the effects mediated by Tbx3 on the master osteogenic transcription factor Runx2 in C3h10t1/2 cells...
  61. Miyazaki T, Kanatani N, Rokutanda S, Yoshida C, Toyosawa S, Nakamura R, et al. Inhibition of the terminal differentiation of odontoblasts and their transdifferentiation into osteoblasts in Runx2 transgenic mice. Arch Histol Cytol. 2008;71:131-46 pubmed
    b>Runx2 is an essential transcription factor for bone and tooth development whose function in odontoblast differentiation remains to be clarified...
  62. Yang F, Tang W, So S, de Crombrugghe B, Zhang C. Sclerostin is a direct target of osteoblast-specific transcription factor osterix. Biochem Biophys Res Commun. 2010;400:684-8 pubmed publisher
    ..Taken together, these findings support our hypothesis that Sost is a direct target of Osx. This provides a new additional mechanism through which Osx inhibits Wnt signaling during bone formation. ..
  63. Levi B, James A, Nelson E, Brugmann S, Sorkin M, Manu A, et al. Role of Indian hedgehog signaling in palatal osteogenesis. Plast Reconstr Surg. 2011;127:1182-90 pubmed publisher
    ..gene expression during osteogenic differentiation and cellular differentiation (Shh, Ihh, Ptc1, Gli1, Gli2, Gli3, Runx2, Alp, and Col1a1)...
  64. Zhang Y, Hassan M, Xie R, Hawse J, Spelsberg T, Montecino M, et al. Co-stimulation of the bone-related Runx2 P1 promoter in mesenchymal cells by SP1 and ETS transcription factors at polymorphic purine-rich DNA sequences (Y-repeats). J Biol Chem. 2009;284:3125-35 pubmed publisher
    Transcriptional control of Runx2 gene expression through two alternative promoters (P1 and P2) is critical for the execution of its function as an osteogenic cell fate determining factor...
  65. Maruyama Z, Yoshida C, Furuichi T, Amizuka N, Ito M, Fukuyama R, et al. Runx2 determines bone maturity and turnover rate in postnatal bone development and is involved in bone loss in estrogen deficiency. Dev Dyn. 2007;236:1876-90 pubmed
    b>Runx2 is an essential transcription factor for osteoblast differentiation. However, the functions of Runx2 in postnatal bone development remain to be clarified...
  66. Komori T. Regulation of bone development and maintenance by Runx2. Front Biosci. 2008;13:898-903 pubmed
    b>Runx2 and Sp7/Osterix determine the osteoblast lineage from mesenchymal stem cells with canonical Wnt signaling...
  67. Byon C, Sun Y, Chen J, Yuan K, Mao X, Heath J, et al. Runx2-upregulated receptor activator of nuclear factor ?B ligand in calcifying smooth muscle cells promotes migration and osteoclastic differentiation of macrophages. Arterioscler Thromb Vasc Biol. 2011;31:1387-96 pubmed publisher
    ..We have previously determined that the osteogenic transcription factor Runx2 is essential for VSMC calcification...
  68. de Frutos C, Dacquin R, Vega S, Jurdic P, Machuca Gayet I, Nieto M. Snail1 controls bone mass by regulating Runx2 and VDR expression during osteoblast differentiation. EMBO J. 2009;28:686-96 pubmed publisher
    ..level, Snail1 controls bone mass by repressing the transcription of both the osteoblast differentiation factor Runx2 and the vitamin D receptor (VDR) genes in osteoblasts...
  69. Cui R, Li S, Liu L, Yi L, Liang Q, Zhu X, et al. MicroRNA-204 regulates vascular smooth muscle cell calcification in vitro and in vivo. Cardiovasc Res. 2012;96:320-9 pubmed publisher
    ..found that miR-204 was suppressed in mouse aortic VSMCs during ?-glycerophosphate-induced calcification, whereas Runx2 protein levels were elevated...
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