Runx1

Summary

Gene Symbol: Runx1
Description: runt related transcription factor 1
Alias: AML1, CBF-alpha-2, Cbfa2, Pebp2a2, Pebpa2b, runt-related transcription factor 1, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit, acute myeloid leukemia 1 protein, core binding factor alpha 2, core-binding factor subunit alpha-2, oncogene AML-1, polyomavirus enhancer-binding protein 2 alpha B subunit, runt domain, alpha subunit 2
Species: mouse
Products:     Runx1

Top Publications

  1. Lian J, Balint E, Javed A, Drissi H, Vitti R, Quinlan E, et al. Runx1/AML1 hematopoietic transcription factor contributes to skeletal development in vivo. J Cell Physiol. 2003;196:301-11 pubmed
    ..Here we addressed expression of the hematopoietic family member Runx1 (AML1/Cbfa2) in relation to skeletal development using a LacZ knock-in mouse model (Runx1(lz/+))...
  2. Rudra D, Egawa T, Chong M, Treuting P, Littman D, Rudensky A. Runx-CBFbeta complexes control expression of the transcription factor Foxp3 in regulatory T cells. Nat Immunol. 2009;10:1170-7 pubmed publisher
    ..Thus, Runx-CBFbeta complexes maintain stable high expression of Foxp3 and serve as an essential determinant of T(reg) cell lineage stability. ..
  3. Chen C, Broom D, Liu Y, de Nooij J, Li Z, Cen C, et al. Runx1 determines nociceptive sensory neuron phenotype and is required for thermal and neuropathic pain. Neuron. 2006;49:365-77 pubmed
    ..We show here that Runx1, a Runt domain transcription factor, is expressed in most nociceptors during embryonic development but in adult ..
  4. Wong W, Kurokawa M, Satake M, Kohu K. Down-regulation of Runx1 expression by TCR signal involves an autoregulatory mechanism and contributes to IL-2 production. J Biol Chem. 2011;286:11110-8 pubmed publisher
    b>Runx1 transcription factor plays multiple roles in T cell development, differentiation, and function...
  5. Swiers G, de Bruijn M, Speck N. Hematopoietic stem cell emergence in the conceptus and the role of Runx1. Int J Dev Biol. 2010;54:1151-63 pubmed publisher
    ..One molecule that has been a particularly useful marker of this developmental event in multiple species is Runx1 (also known as AML1, Pebp2alpha)...
  6. Motoda L, Osato M, Yamashita N, Jacob B, Chen L, Yanagida M, et al. Runx1 protects hematopoietic stem/progenitor cells from oncogenic insult. Stem Cells. 2007;25:2976-86 pubmed
    The RUNX1/AML1 gene encodes a transcription factor essential for the generation of hematopoietic stem cells and is frequently targeted in human leukemia...
  7. Okada H, Watanabe T, Niki M, Takano H, Chiba N, Yanai N, et al. AML1(-/-) embryos do not express certain hematopoiesis-related gene transcripts including those of the PU.1 gene. Oncogene. 1998;17:2287-93 pubmed
    The AML1 and PEBP2beta/CBFbeta genes encode the DNA-binding and non-binding subunits, respectively, of the heterodimeric transcription factor, PEBP2/CBF...
  8. Jo M, Curry T. Luteinizing hormone-induced RUNX1 regulates the expression of genes in granulosa cells of rat periovulatory follicles. Mol Endocrinol. 2006;20:2156-72 pubmed
    ..The present study determined 1) the localization of RUNX1, a nuclear transcription factor, 2) regulation of Runx1 mRNA expression, and 3) its potential function in rat ..
  9. Dykes I, Lanier J, Eng S, Turner E. Brn3a regulates neuronal subtype specification in the trigeminal ganglion by promoting Runx expression during sensory differentiation. Neural Dev. 2010;5:3 pubmed publisher
    ..In embryonic Brn3a-/- ganglia, the normal expression of Runx3 is never initiated in TrkC+ neurons, and Runx1 expression is greatly attenuated in TrkA+ nociceptors...

More Information

Publications95

  1. Liakhovitskaia A, Gribi R, Stamateris E, Villain G, Jaffredo T, Wilkie R, et al. Restoration of Runx1 expression in the Tie2 cell compartment rescues definitive hematopoietic stem cells and extends life of Runx1 knockout animals until birth. Stem Cells. 2009;27:1616-24 pubmed publisher
    Mice deficient in the runt homology domain transcription factor Runx1/AML1 fail to generate functional clonogenic hematopoietic cells and die in utero by embryonic day 12.5...
  2. Levanon D, Negreanu V, Bernstein Y, Bar Am I, Avivi L, Groner Y. AML1, AML2, and AML3, the human members of the runt domain gene-family: cDNA structure, expression, and chromosomal localization. Genomics. 1994;23:425-32 pubmed
    cDNAs corresponding to three human runt domain containing genes, AML1, AML2, and AML3, were isolated and characterized...
  3. Tanaka Y, Hayashi M, Kubota Y, Nagai H, Sheng G, Nishikawa S, et al. Early ontogenic origin of the hematopoietic stem cell lineage. Proc Natl Acad Sci U S A. 2012;109:4515-20 pubmed publisher
    ..To deconvolute hematopoietic ontogeny, we designed an embryo-rescue system in which the key hematopoietic factor Runx1 is reactivated in Runx1-null conceptuses at specific developmental stages...
  4. Bae S, Ogawa E, Maruyama M, Oka H, Satake M, Shigesada K, et al. PEBP2 alpha B/mouse AML1 consists of multiple isoforms that possess differential transactivation potentials. Mol Cell Biol. 1994;14:3242-52 pubmed
    ..The last two genes are syntenic with the human AML1 on chromosome 21q22 and PEBP2 beta/CBF beta on 16q22 detected at the breakpoints of characteristic chromosome ..
  5. Sroczynska P, Lancrin C, Kouskoff V, Lacaud G. The differential activities of Runx1 promoters define milestones during embryonic hematopoiesis. Blood. 2009;114:5279-89 pubmed publisher
    The transcription factor RUNX1/AML1 is a master regulator of hematopoietic development...
  6. Chakraborty S, Sinha K, Senyuk V, Nucifora G. SUV39H1 interacts with AML1 and abrogates AML1 transactivity. AML1 is methylated in vivo. Oncogene. 2003;22:5229-37 pubmed
    Acute myeloid leukemia 1 (AML1) belongs to a family of DNA-binding proteins highly conserved through evolution. AML1 regulates the expression of several hematopoietic genes and is essential for murine fetal liver hematopoiesis...
  7. Raveh E, Cohen S, Levanon D, Negreanu V, Groner Y, Gat U. Dynamic expression of Runx1 in skin affects hair structure. Mech Dev. 2006;123:842-50 pubmed
    ..Here, we report the expression of Runx1 during the development of hair and other skin appendages in the mouse and describe the effect of Runx1 on the ..
  8. Hoi C, Lee S, Lu S, McDermitt D, Osorio K, Piskun C, et al. Runx1 directly promotes proliferation of hair follicle stem cells and epithelial tumor formation in mouse skin. Mol Cell Biol. 2010;30:2518-36 pubmed publisher
    b>Runx1/AML1 is a transcription factor implicated in tissue stem cell regulation and belongs to the small Runx family of cancer genes...
  9. Kim W, Sieweke M, Ogawa E, Wee H, Englmeier U, Graf T, et al. Mutual activation of Ets-1 and AML1 DNA binding by direct interaction of their autoinhibitory domains. EMBO J. 1999;18:1609-20 pubmed
    The transcription factors Ets-1 and AML1 (the alphaBl subunit of PEBP2/CBF) play critical roles in hematopoiesis and leukemogenesis, and cooperate in the transactivation of the T cell receptor (TCR) beta chain enhancer...
  10. Giese K, Kingsley C, Kirshner J, Grosschedl R. Assembly and function of a TCR alpha enhancer complex is dependent on LEF-1-induced DNA bending and multiple protein-protein interactions. Genes Dev. 1995;9:995-1008 pubmed
  11. Buchholz F, Refaeli Y, Trumpp A, Bishop J. Inducible chromosomal translocation of AML1 and ETO genes through Cre/loxP-mediated recombination in the mouse. EMBO Rep. 2000;1:133-9 pubmed
    ..As in the human tumors, the murine translocation fuses the genes AML1 and ETO...
  12. Lichtinger M, Ingram R, Hannah R, Müller D, Clarke D, Assi S, et al. RUNX1 reshapes the epigenetic landscape at the onset of haematopoiesis. EMBO J. 2012;31:4318-33 pubmed publisher
    Cell fate decisions during haematopoiesis are governed by lineage-specific transcription factors, such as RUNX1, SCL/TAL1, FLI1 and C/EBP family members...
  13. Zovein A, Hofmann J, Lynch M, French W, Turlo K, Yang Y, et al. Fate tracing reveals the endothelial origin of hematopoietic stem cells. Cell Stem Cell. 2008;3:625-36 pubmed publisher
    ..Moreover, AGM mesenchyme, as selectively traced via a myocardin Cre line, is incapable of hematopoiesis. Our genetic tracing strategy therefore reveals an endothelial origin of HSCs...
  14. Lorsbach R, Moore J, Ang S, Sun W, Lenny N, Downing J. Role of RUNX1 in adult hematopoiesis: analysis of RUNX1-IRES-GFP knock-in mice reveals differential lineage expression. Blood. 2004;103:2522-9 pubmed
    The Runx1/core binding factor-beta (CBFbeta) transcriptional complex is required for the establishment of hematopoiesis during development...
  15. Theriault F, Roy P, Stifani S. AML1/Runx1 is important for the development of hindbrain cholinergic branchiovisceral motor neurons and selected cranial sensory neurons. Proc Natl Acad Sci U S A. 2004;101:10343-8 pubmed
    ..It is shown here that the mammalian runt-related gene Runx1 is expressed in selected populations of postmitotic neurons of the embryonic central and peripheral nervous ..
  16. Tsuzuki S, Hong D, Gupta R, Matsuo K, Seto M, Enver T. Isoform-specific potentiation of stem and progenitor cell engraftment by AML1/RUNX1. PLoS Med. 2007;4:e172 pubmed
    AML1/RUNX1 is the most frequently mutated gene in leukaemia and is central to the normal biology of hematopoietic stem and progenitor cells. However, the role of different AML1 isoforms within these primitive compartments is unclear...
  17. de Guzman C, Johnson A, Klug C. The ETO domain is necessary for the developmental abnormalities associated with AML1-ETO expression in the hematopoietic stem cell compartment in vivo. Blood Cells Mol Dis. 2003;30:201-6 pubmed
    Translocation of the ETO gene on human chromosome 8 with the AML1 gene on chromosome 21 (AML1-ETO) is a recurrent cytogenetic abnormality associated with approximately 12% of acute myelogenous leukemia (AML) cases...
  18. Levanon D, Bettoun D, Harris Cerruti C, Woolf E, Negreanu V, Eilam R, et al. The Runx3 transcription factor regulates development and survival of TrkC dorsal root ganglia neurons. EMBO J. 2002;21:3454-63 pubmed
    ..The data provide new genetic insights into the neurogenesis of DRGs and may help elucidate the molecular mechanisms underlying somatosensory-related ataxia in humans. ..
  19. Fujita Y, Nishimura M, Taniwaki M, Abe T, Okuda T. Identification of an alternatively spliced form of the mouse AML1/RUNX1 gene transcript AML1c and its expression in early hematopoietic development. Biochem Biophys Res Commun. 2001;281:1248-55 pubmed
    Acute myeloid leukemia 1 (AML1: or runt-related transcription factor, RUNX1) encodes the DNA binding subunit of the heterodimering transcription factor complex PEBP2 (CBF), which plays an essential role for definitive hematopoiesis...
  20. North T, Gu T, Stacy T, Wang Q, Howard L, Binder M, et al. Cbfa2 is required for the formation of intra-aortic hematopoietic clusters. Development. 1999;126:2563-75 pubmed
    b>Cbfa2 (AML1) encodes the DNA-binding subunit of a transcription factor in the small family of core-binding factors (CBFs)...
  21. Hirai H, Samokhvalov I, Fujimoto T, Nishikawa S, Imanishi J, Nishikawa S. Involvement of Runx1 in the down-regulation of fetal liver kinase-1 expression during transition of endothelial cells to hematopoietic cells. Blood. 2005;106:1948-55 pubmed
    ..early mouse embryogenesis, fetal liver kinase-1 (Flk-1), a receptor for vascular endothelial growth factor, and Runx1, a runt domain transcription factor, have prerequisite roles in the generation of hematopoietic lineages...
  22. Landry J, Kinston S, Knezevic K, de Bruijn M, Wilson N, Nottingham W, et al. Runx genes are direct targets of Scl/Tal1 in the yolk sac and fetal liver. Blood. 2008;111:3005-14 pubmed publisher
    Transcription factors such as Scl/Tal1, Lmo2, and Runx1 are essential for the development of hematopoietic stem cells (HSCs)...
  23. Bee T, Swiers G, Muroi S, Pozner A, Nottingham W, Santos A, et al. Nonredundant roles for Runx1 alternative promoters reflect their activity at discrete stages of developmental hematopoiesis. Blood. 2010;115:3042-50 pubmed publisher
    The transcription factor Runx1 is a pivotal regulator of definitive hematopoiesis in mouse ontogeny...
  24. Cai Z, de Bruijn M, Ma X, Dortland B, Luteijn T, Downing R, et al. Haploinsufficiency of AML1 affects the temporal and spatial generation of hematopoietic stem cells in the mouse embryo. Immunity. 2000;13:423-31 pubmed
    The AML1:CBFbeta transcription factor complex is essential for definitive hematopoiesis. Null mutations in mouse AML1 result in midgestational lethality with a complete lack of fetal liver hematopoiesis...
  25. Lancrin C, Sroczynska P, Stephenson C, Allen T, Kouskoff V, Lacaud G. The haemangioblast generates haematopoietic cells through a haemogenic endothelium stage. Nature. 2009;457:892-5 pubmed publisher
    ..is indispensable for the establishment of this haemogenic endothelium population whereas the core binding factor Runx1 (also known as AML1; ref...
  26. Luo W, Wickramasinghe S, Savitt J, Griffin J, Dawson T, Ginty D. A hierarchical NGF signaling cascade controls Ret-dependent and Ret-independent events during development of nonpeptidergic DRG neurons. Neuron. 2007;54:739-54 pubmed
    ..other genes characteristic of nonpeptidergic neurons, such as TrpC3, TrpM8, MrgD, and the transcription factor Runx1, via a Ret-independent signaling pathway...
  27. Naoe Y, Setoguchi R, Akiyama K, Muroi S, Kuroda M, Hatam F, et al. Repression of interleukin-4 in T helper type 1 cells by Runx/Cbf beta binding to the Il4 silencer. J Exp Med. 2007;204:1749-55 pubmed
    ..These results demonstrate critical roles of Runx complexes in regulating immune responses, at least in part, through the repression of the Il4 gene. ..
  28. Egawa T, Tillman R, Naoe Y, Taniuchi I, Littman D. The role of the Runx transcription factors in thymocyte differentiation and in homeostasis of naive T cells. J Exp Med. 2007;204:1945-57 pubmed
    ..We used a strategy to conditionally inactivate the genes encoding Runx1 or Runx3 at different stages of thymocyte development, demonstrating that Runx1 regulates the transitions of ..
  29. Challen G, Goodell M. Runx1 isoforms show differential expression patterns during hematopoietic development but have similar functional effects in adult hematopoietic stem cells. Exp Hematol. 2010;38:403-16 pubmed publisher
    b>RUNX1 (also known as acute myeloid leukemia 1) is an essential regulator of hematopoiesis and has multiple isoforms arising from differential splicing and utilization of two promoters...
  30. Schindler J, Van Buren D, Foudi A, Krejci O, Qin J, Orkin S, et al. TEL-AML1 corrupts hematopoietic stem cells to persist in the bone marrow and initiate leukemia. Cell Stem Cell. 2009;5:43-53 pubmed publisher
    The initial steps in the pathogenesis of acute leukemia remain incompletely understood. The TEL-AML1 gene fusion, the hallmark translocation in Childhood Acute Lymphoblastic Leukemia and the first hit, occurs years before the clinical ..
  31. Rhodes K, Gekas C, Wang Y, Lux C, Francis C, Chan D, et al. The emergence of hematopoietic stem cells is initiated in the placental vasculature in the absence of circulation. Cell Stem Cell. 2008;2:252-63 pubmed publisher
    ..By tracking developing HSCs by expression of Runx1-lacZ and CD41, we have found that HSCs emerge in large vessels in the placenta...
  32. Matsuura S, Komeno Y, Stevenson K, Biggs J, Lam K, Tang T, et al. Expression of the runt homology domain of RUNX1 disrupts homeostasis of hematopoietic stem cells and induces progression to myelodysplastic syndrome. Blood. 2012;120:4028-37 pubmed publisher
    Mutations of RUNX1 are detected in patients with myelodysplastic syndrome (MDS). In particular, C-terminal truncation mutations lack a transcription regulatory domain and have increased DNA binding through the runt homology domain...
  33. Swiers G, Baumann C, O Rourke J, Giannoulatou E, Taylor S, Joshi A, et al. Early dynamic fate changes in haemogenic endothelium characterized at the single-cell level. Nat Commun. 2013;4:2924 pubmed publisher
    ..We have generated a Runx1 +23 enhancer-reporter transgenic mouse (23GFP) for the prospective isolation of HE throughout embryonic ..
  34. Hayashi K, Abe N, Watanabe T, Obinata M, Ito M, Sato T, et al. Overexpression of AML1 transcription factor drives thymocytes into the CD8 single-positive lineage. J Immunol. 2001;167:4957-65 pubmed
    ..involved in the development of single-positive (SP) thymocytes, we generated transgenic mice in which the AML1 transcription factor is overexpressed...
  35. Sun W, Graves B, Speck N. Transactivation of the Moloney murine leukemia virus and T-cell receptor beta-chain enhancers by cbf and ets requires intact binding sites for both proteins. J Virol. 1995;69:4941-9 pubmed
    ..Moreover, we show that transactivation by Cbf alpha 2 requires both intact ets and cbf binding sites...
  36. Simeone A, Daga A, Calabi F. Expression of runt in the mouse embryo. Dev Dyn. 1995;203:61-70 pubmed
    The Drosophila runt locus controls early events in embryogenesis. A human homologue (CBFA2) was originally identified because of its involvement in the t(8;21) associated with a subtype of acute myeloid leukaemia...
  37. Sun W, Downing J. Haploinsufficiency of AML1 results in a decrease in the number of LTR-HSCs while simultaneously inducing an increase in more mature progenitors. Blood. 2004;104:3565-72 pubmed
    The AML1/CBFbeta transcriptional complex is essential for the formation of definitive hematopoietic stem cells (HSCs). Moreover, development of the hematopoietic system is exquisitely sensitive to the level of this complex...
  38. Ichikawa M, Goyama S, Asai T, Kawazu M, Nakagawa M, Takeshita M, et al. AML1/Runx1 negatively regulates quiescent hematopoietic stem cells in adult hematopoiesis. J Immunol. 2008;180:4402-8 pubmed
    Transcription factor AML1/Runx1, initially isolated from the t(8;21) chromosomal translocation in human leukemia, is essential for the development of multilineage hematopoiesis in mouse embryos...
  39. North T, de Bruijn M, Stacy T, Talebian L, Lind E, Robin C, et al. Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo. Immunity. 2002;16:661-72 pubmed
    ..b>Runx1 (AML1), the DNA binding subunit of a core binding factor, is required for the emergence and/or subsequent function ..
  40. Taniuchi I, Osato M, Egawa T, Sunshine M, Bae S, Komori T, et al. Differential requirements for Runx proteins in CD4 repression and epigenetic silencing during T lymphocyte development. Cell. 2002;111:621-33 pubmed
    ..b>Runx1 is required for active repression in CD4(-)CD8(-) thymocytes whereas Runx3 is required for establishing epigenetic ..
  41. Nagamachi A, Htun P, Ma F, Miyazaki K, Yamasaki N, Kanno M, et al. A 5' untranslated region containing the IRES element in the Runx1 gene is required for angiogenesis, hematopoiesis and leukemogenesis in a knock-in mouse model. Dev Biol. 2010;345:226-36 pubmed publisher
    ..b>Runx1 gene, which encodes a transcription factor implicated in hematopoiesis, angiogenesis, and leukemogenesis, contains ..
  42. Zovein A, Turlo K, Ponec R, Lynch M, Chen K, Hofmann J, et al. Vascular remodeling of the vitelline artery initiates extravascular emergence of hematopoietic clusters. Blood. 2010;116:3435-44 pubmed publisher
    ..75 in the caudal half of the midgut mesentery, but by embryonic day 11.0 are sporadically found on the cranial side of the midgut, thus suggesting possible extravascular migration aided by midgut rotation...
  43. Gascon E, Gaillard S, Malapert P, Liu Y, Rodat Despoix L, Samokhvalov I, et al. Hepatocyte growth factor-Met signaling is required for Runx1 extinction and peptidergic differentiation in primary nociceptive neurons. J Neurosci. 2010;30:12414-23 pubmed publisher
    ..They can be divided into the following two major classes: peptidergic and nonpeptidergic neurons. Although RUNX1 has been shown to play a pivotal role in the specification of nonpeptidergic neurons, the mechanisms driving ..
  44. Tachibana M, Tenno M, Tezuka C, Sugiyama M, Yoshida H, Taniuchi I. Runx1/Cbf?2 complexes are required for lymphoid tissue inducer cell differentiation at two developmental stages. J Immunol. 2011;186:1450-7 pubmed publisher
    ..In this study, we report impaired lymphoid tissue organogenesis in mice in which the function of Runx1/Cbf? transcription factor complexes was attenuated by the loss of either the distal promoter-derived Runx1 or Cbf?..
  45. Kataoka H, Hayashi M, Nakagawa R, Tanaka Y, Izumi N, Nishikawa S, et al. Etv2/ER71 induces vascular mesoderm from Flk1+PDGFR?+ primitive mesoderm. Blood. 2011;118:6975-86 pubmed publisher
    ..We propose that Flk-1(+)/PDGFR?(+) primitive mesoderm is committed into Flk-1(+)/PDGFR?(-) vascular mesoderm through Etv2 and that up-regulation of Etv2 by VEGF promotes this commitment...
  46. Abdel Samad O, Liu Y, Yang F, Kramer I, Arber S, Ma Q. Characterization of two Runx1-dependent nociceptor differentiation programs necessary for inflammatory versus neuropathic pain. Mol Pain. 2010;6:45 pubmed publisher
    ..We reported previously that the runt domain transcription factor Runx1 is initially expressed in most nociceptors and controls sensory neuron phenotypes necessary for inflammatory and ..
  47. Pozner A, Lotem J, Xiao C, Goldenberg D, Brenner O, Negreanu V, et al. Developmentally regulated promoter-switch transcriptionally controls Runx1 function during embryonic hematopoiesis. BMC Dev Biol. 2007;7:84 pubmed
    ..b>Runx1 transcription factor is a key regulator of early hematopoiesis and a frequent target of chromosomal translocations ..
  48. Huang G, Shigesada K, Ito K, Wee H, Yokomizo T, Ito Y. Dimerization with PEBP2beta protects RUNX1/AML1 from ubiquitin-proteasome-mediated degradation. EMBO J. 2001;20:723-33 pubmed
    ..The beta subunit consists of the non-RUNX protein PEBP2beta. We found that RUNX1/AML1, which is essential for hematopoiesis, is continuously subjected to proteolytic degradation mediated by the ..
  49. Wotton S, Stewart M, Blyth K, Vaillant F, Kilbey A, Neil J, et al. Proviral insertion indicates a dominant oncogenic role for Runx1/AML-1 in T-cell lymphoma. Cancer Res. 2002;62:7181-5 pubmed
    The RUNX1/AML1 gene is a frequent target for chromosomal translocations in human leukemia. The biological properties of the resulting fusion products and the finding that haploinsufficiency increases the risk of developing leukemia (W-J...
  50. Wong W, Nakazato M, Watanabe T, Kohu K, Ogata T, Yoshida N, et al. Over-expression of Runx1 transcription factor impairs the development of thymocytes from the double-negative to double-positive stages. Immunology. 2010;130:243-53 pubmed publisher
    b>Runx1 transcription factor is highly expressed at a CD4/CD8-double-negative (DN) stage of thymocyte development but is down-regulated when cells proceed to the double-positive (DP) stage...
  51. Yoshikawa M, Senzaki K, Yokomizo T, Takahashi S, Ozaki S, Shiga T. Runx1 selectively regulates cell fate specification and axonal projections of dorsal root ganglion neurons. Dev Biol. 2007;303:663-74 pubmed
    b>Runx1-deficient mice die around embryonic day 11.5 due to impaired hematopoiesis. This early death prevents the analysis of the role of Runx1 in the development of sensory ganglia...
  52. Levanon D, Brenner O, Negreanu V, Bettoun D, Woolf E, Eilam R, et al. Spatial and temporal expression pattern of Runx3 (Aml2) and Runx1 (Aml1) indicates non-redundant functions during mouse embryogenesis. Mech Dev. 2001;109:413-7 pubmed
    ..Here, we describe the expression pattern of Runx3 during mouse embryogenesis compared to the expression pattern of Runx1. E10.5 and E14.5-E16...
  53. Yokomizo T, Takahashi S, Mochizuki N, Kuroha T, Ema M, Wakamatsu A, et al. Characterization of GATA-1(+) hemangioblastic cells in the mouse embryo. EMBO J. 2007;26:184-96 pubmed
    ..a transgenic complementation rescue approach, GATA-1(+) cell-derived progenitors were selectively restored in Runx1-deficient mice...
  54. Ginhoux F, Greter M, Leboeuf M, Nandi S, See P, Gokhan S, et al. Fate mapping analysis reveals that adult microglia derive from primitive macrophages. Science. 2010;330:841-5 pubmed publisher
    ..These results identify microglia as an ontogenically distinct population in the mononuclear phagocyte system and have implications for the use of embryonically derived microglial progenitors for the treatment of various brain disorders...
  55. Wang Q, Stacy T, Miller J, Lewis A, Gu T, Huang X, et al. The CBFbeta subunit is essential for CBFalpha2 (AML1) function in vivo. Cell. 1996;87:697-708 pubmed
    ..Genes encoding the CBFbeta subunit (CBFB) and one of the CBFalpha subunits (CBFA2, otherwise known as AML1) are the most frequent targets of chromosomal translocations in acute leukemias in humans...
  56. Miyoshi H, Shimizu K, Kozu T, Maseki N, Kaneko Y, Ohki M. t(8;21) breakpoints on chromosome 21 in acute myeloid leukemia are clustered within a limited region of a single gene, AML1. Proc Natl Acad Sci U S A. 1991;88:10431-4 pubmed
    ..We report here the cloning of a gene, named AML1, on chromosome 21 that was found to be rearranged in the leukemic cell DNAs from t(8;21) AML patients...
  57. Hayashi K, Natsume W, Watanabe T, Abe N, Iwai N, Okada H, et al. Diminution of the AML1 transcription factor function causes differential effects on the fates of CD4 and CD8 single-positive T cells. J Immunol. 2000;165:6816-24 pubmed
    ..We examined the role of AML1, the gene encoding a transcription factor, in the above processes by using the transgenic mice expressing a ..
  58. Klunker S, Chong M, Mantel P, Palomares O, Bassin C, Ziegler M, et al. Transcription factors RUNX1 and RUNX3 in the induction and suppressive function of Foxp3+ inducible regulatory T cells. J Exp Med. 2009;206:2701-15 pubmed publisher
    ..the transforming growth factor-beta (TGF-beta) induces the expression of the Runt-related transcription factors RUNX1 and RUNX3 in CD4(+) T cells...
  59. Wang S, Wang Q, Crute B, Melnikova I, Keller S, Speck N. Cloning and characterization of subunits of the T-cell receptor and murine leukemia virus enhancer core-binding factor. Mol Cell Biol. 1993;13:3324-39 pubmed
    ..Association of the CBF beta subunits does not extend the phosphate contacts in the binding site. We propose that CBF beta is a non-DNA-binding subunit of CBF and does not contact DNA directly. ..
  60. Hoogenkamp M, Lichtinger M, Krysinska H, Lancrin C, Clarke D, Williamson A, et al. Early chromatin unfolding by RUNX1: a molecular explanation for differential requirements during specification versus maintenance of the hematopoietic gene expression program. Blood. 2009;114:299-309 pubmed publisher
    ..1, its target gene colony-stimulating-factor 1 receptor (Csf1r), and key upstream regulators such as RUNX1. We find that during ontogeny, chromatin unfolding precedes the establishment of active histone marks and the ..
  61. Yokomizo T, Hasegawa K, Ishitobi H, Osato M, Ema M, Ito Y, et al. Runx1 is involved in primitive erythropoiesis in the mouse. Blood. 2008;111:4075-80 pubmed publisher
    Targeted disruption of the Runx1/ AML1 gene in mice has demonstrated that it is required for the emergence of definitive hematopoietic cells but that it is not essential for the formation of primitive erythrocytes...
  62. Tahirov T, Inoue Bungo T, Morii H, Fujikawa A, Sasaki M, Kimura K, et al. Structural analyses of DNA recognition by the AML1/Runx-1 Runt domain and its allosteric control by CBFbeta. Cell. 2001;104:755-67 pubmed
    ..Here, we present the crystal structures of the AML1/Runx-1/CBFalpha(Runt domain)-CBFbeta(core domain)-C/EBPbeta(bZip)-DNA, AML1/Runx-1/CBFalpha(Runt domain)-C/EBPbeta(..
  63. Wang X, Blagden C, Fan J, Nowak S, Taniuchi I, Littman D, et al. Runx1 prevents wasting, myofibrillar disorganization, and autophagy of skeletal muscle. Genes Dev. 2005;19:1715-22 pubmed
    ..Previously, we found that Runx1 (AML1), a DNA-binding protein that is homologous to Drosophila Runt and has critical roles in hematopoiesis and ..
  64. Yanagida M, Osato M, Yamashita N, Liqun H, Jacob B, Wu F, et al. Increased dosage of Runx1/AML1 acts as a positive modulator of myeloid leukemogenesis in BXH2 mice. Oncogene. 2005;24:4477-85 pubmed
    The RUNX1/AML1 gene on chromosome 21 is most frequently inactivated in human leukemias. In addition, an increased dose of RUNX1 is suggested as a basis for several kinds of leukemias...
  65. Theriault F, Nuthall H, Dong Z, Lo R, Barnabe Heider F, Miller F, et al. Role for Runx1 in the proliferation and neuronal differentiation of selected progenitor cells in the mammalian nervous system. J Neurosci. 2005;25:2050-61 pubmed
    ..It is shown here that the murine runt-related gene Runx1 is expressed in proliferating cells on the basal side of the olfactory epithelium...
  66. Iwatsuki K, Tanaka K, Kaneko T, Kazama R, Okamoto S, Nakayama Y, et al. Runx1 promotes angiogenesis by downregulation of insulin-like growth factor-binding protein-3. Oncogene. 2005;24:1129-37 pubmed
    Mouse embryos lacking the Runx1 transcription factor exhibit an angiogenic defect accompanied by the absence of hematopoietic stem cells (HSCs)...
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    The transcription factor Runx1 plays a pivotal role in hematopoietic stem cell (HSC) emergence, and studies into its transcriptional regulation should give insight into the critical steps of HSC specification...
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    ..Our results suggest that MHC class II-selected thymocytes are directed toward the CD4(+) lineage independently of ThPOK but require ThPOK to prevent Runx-dependent differentiation toward the CD8(+) lineage. ..
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    ..We propose mechanisms by which mutations in the Runt domain found in hematopoietic and bone disorders affect its affinity for DNA. ..
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    As reported previously, AML1-ETO knock-in mice were generated to investigate the role of AML1-ETO in leukemogenesis and to mimic the progression of t(8;21) leukemia...
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    AML1/RUNX1, a member of the core binding factor (CBF) family stimulates myelopoiesis and lymphopoiesis by activating lineage-specific genes...
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    ..In this regard, the gene encoding the transcription factor Runx1 has emerged as a specific marker of restricted neuronal populations in the murine central and peripheral nervous ..
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    ..We conclude that the mouse embryonic head is a previously unappreciated site for HSC emergence within the developing embryo...
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    ..EBF function was enhanced by interaction with the transcription factor Runx1. These data suggest a molecular basis for the hierarchical dependence of Pax5 function on EBF and E2A in B ..
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    ..The Runt-related transcription factors, Runx1 and Runx3, are expressed in DRG neuronal subpopulations, suggesting that they might regulate the cell ..
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    Core Binding Factor or CBF is a transcription factor composed of two subunits, Runx1/AML-1 and CBF beta or CBFbeta. CBF was originally described as a regulator of hematopoiesis...
  78. Van Handel B, Montel Hagen A, Sasidharan R, Nakano H, Ferrari R, Boogerd C, et al. Scl represses cardiomyogenesis in prospective hemogenic endothelium and endocardium. Cell. 2012;150:590-605 pubmed publisher
    ..These results reveal unexpected plasticity in embryonic endothelium such that loss of a single master regulator can induce ectopic cardiomyogenesis from endothelial cells. ..
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    ..Here we show that the mouse transcription factor Runx1, a key regulator of myeloid cell proliferation and differentiation, is expressed in forebrain amoeboid microglia ..
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    The Runt family transcription factor CBFalpha2 (AML1, PEBP2alphaB, or Runx1) is required by hematopoietic stem cells and expressed at high levels in T-lineage cells...
  81. Ono M, Yaguchi H, Ohkura N, Kitabayashi I, Nagamura Y, Nomura T, et al. Foxp3 controls regulatory T-cell function by interacting with AML1/Runx1. Nature. 2007;446:685-9 pubmed
    ..Here we show that the transcription factor AML1 (acute myeloid leukaemia 1)/Runx1 (Runt-related transcription factor 1), which is crucially required for normal haematopoiesis including thymic T-..
  82. Levantini E, Lee S, Radomska H, Hetherington C, Alberich Jorda M, Amabile G, et al. RUNX1 regulates the CD34 gene in haematopoietic stem cells by mediating interactions with a distal regulatory element. EMBO J. 2011;30:4059-70 pubmed publisher
    The transcription factor RUNX1 is essential to establish the haematopoietic gene expression programme; however, the mechanism of how it activates transcription of haematopoietic stem cell (HSC) genes is still elusive...
  83. Tober J, Yzaguirre A, Piwarzyk E, Speck N. Distinct temporal requirements for Runx1 in hematopoietic progenitors and stem cells. Development. 2013;140:3765-76 pubmed publisher
    The transcription factor Runx1 is essential for the formation of yolk sac-derived erythroid/myeloid progenitors (EMPs) and hematopoietic stem cells (HSCs) from hemogenic endothelium during embryogenesis...
  84. Nottingham W, Jarratt A, Burgess M, Speck C, Cheng J, Prabhakar S, et al. Runx1-mediated hematopoietic stem-cell emergence is controlled by a Gata/Ets/SCL-regulated enhancer. Blood. 2007;110:4188-97 pubmed
    The transcription factor Runx1/AML1 is an important regulator of hematopoiesis and is critically required for the generation of the first definitive hematopoietic stem cells (HSCs) in the major vasculature of the mouse embryo...
  85. Zhang F, Meng G, Strober W. Interactions among the transcription factors Runx1, RORgammat and Foxp3 regulate the differentiation of interleukin 17-producing T cells. Nat Immunol. 2008;9:1297-306 pubmed publisher
    ..Both cis-regulatory elements contained regions that bound the transcription factors RORgammat and Runx1. Runx1 influenced T(H)-17 differentiation by inducing RORgammat expression and by binding to and acting together ..
  86. Nishimura M, Fukushima Nakase Y, Fujita Y, Nakao M, Toda S, Kitamura N, et al. VWRPY motif-dependent and -independent roles of AML1/Runx1 transcription factor in murine hematopoietic development. Blood. 2004;103:562-70 pubmed
    AML1/Runx1 is a frequent target of leukemia-associated gene aberration, and it encodes a transcription factor essential for definitive hematopoiesis...