Gene Symbol: Rpa1
Description: replication protein A1
Alias: 5031405K23Rik, 70kDa, AA589576, AW557552, RF-A, RP-A, Rpa, replication protein A 70 kDa DNA-binding subunit, RF-A protein 1, RP-A p70, replication factor A protein 1
Species: mouse
Products:     Rpa1

Top Publications

  1. Hamilton B, Smith D, Mueller K, Kerrebrock A, Bronson R, van Berkel V, et al. The vibrator mutation causes neurodegeneration via reduced expression of PITP alpha: positional complementation cloning and extragenic suppression. Neuron. 1997;18:711-22 pubmed
    ..The vibrator phenotype is suppressed in one intercross. We performed a complete genome scan and mapped a major suppressor locus (Mvb-1) to proximal chromosome 19. ..
  2. Lipkin S, Moens P, Wang V, Lenzi M, Shanmugarajah D, Gilgeous A, et al. Meiotic arrest and aneuploidy in MLH3-deficient mice. Nat Genet. 2002;31:385-90 pubmed
    ..Mlh3(-/-) spermatocytes reach metaphase before succumbing to apoptosis, but oocytes fail to complete meiosis I after fertilization. Our results show that Mlh3 has an essential and distinct role in mammalian meiosis. ..
  3. Li X, Li X, Schimenti J. Mouse pachytene checkpoint 2 (trip13) is required for completing meiotic recombination but not synapsis. PLoS Genet. 2007;3:e130 pubmed
    ..spermatocytes synapse fully, yet retain several markers of recombination intermediates, including RAD51, BLM, and RPA. These chromosomes also exhibited the chiasmata markers MLH1 and MLH3, and okadaic acid treatment of mutant ..
  4. Kouznetsova A, Benavente R, Pastink A, Hoog C. Meiosis in mice without a synaptonemal complex. PLoS ONE. 2011;6:e28255 pubmed publisher
  5. Herrán Y, Gutiérrez Caballero C, Sanchez Martin M, Hernandez T, Viera A, Barbero J, et al. The cohesin subunit RAD21L functions in meiotic synapsis and exhibits sexual dimorphism in fertility. EMBO J. 2011;30:3091-105 pubmed publisher
    ..Thus, our results provide in vivo evidence that RAD21L is essential for male fertility and in females for the maintenance of fertility during natural aging. ..
  6. Daniel K, Lange J, Hached K, Fu J, Anastassiadis K, Roig I, et al. Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1. Nat Cell Biol. 2011;13:599-610 pubmed publisher
  7. Yang F, Eckardt S, Leu N, McLaughlin K, Wang P. Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis. J Cell Biol. 2008;180:673-9 pubmed publisher
    ..Based on these data, we propose that TEX15 regulates the loading of DNA repair proteins onto sites of DSBs and, thus, its absence causes a failure in meiotic recombination. ..
  8. Fukuda T, Fukuda N, Agostinho A, Hernández Hernández A, Kouznetsova A, Hoog C. STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis. EMBO J. 2014;33:1243-55 pubmed publisher
  9. Dear T, Hainzl T, Follo M, Nehls M, Wilmore H, Matena K, et al. Identification of interaction partners for the basic-helix-loop-helix protein E47. Oncogene. 1997;14:891-8 pubmed
    ..A cDNA encoding part of the nucleolin protein sequence interacted with the E47 basic-helix-loop-helix only when fused to a beta-galactosidase tag. ..

More Information


  1. Kumar R, Bourbon H, de Massy B. Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice. Genes Dev. 2010;24:1266-80 pubmed publisher
    ..in mice, MEI4 is localized in discrete foci on the axes of meiotic chromosomes that do not overlap with DMC1 and RPA foci...
  2. Gong Y, de Lange T. A Shld1-controlled POT1a provides support for repression of ATR signaling at telomeres through RPA exclusion. Mol Cell. 2010;40:377-87 pubmed publisher
    We previously proposed that POT1 prevents ATR signaling at telomeres by excluding RPA from the single-stranded TTAGGG repeats. Here, we use a Shld1-stabilized degron-POT1a fusion (DD-POT1a) to study the telomeric ATR kinase response...
  3. Liu Y, Kvaratskhelia M, Hess S, Qu Y, Zou Y. Modulation of replication protein A function by its hyperphosphorylation-induced conformational change involving DNA binding domain B. J Biol Chem. 2005;280:32775-83 pubmed
    Human replication protein A (RPA), composed of RPA70, RPA32, and RPA14 subunits, undergoes hyperphosphorylation in cells in response to DNA damage...
  4. Wang Y, Putnam C, Kane M, Zhang W, Edelmann L, Russell R, et al. Mutation in Rpa1 results in defective DNA double-strand break repair, chromosomal instability and cancer in mice. Nat Genet. 2005;37:750-5 pubmed
    ..Mice carrying a heterozygous missense change in one of the DNA-binding domains of Rpa1 develop lymphoid tumors, and their homozygous littermates succumb to early embryonic lethality...
  5. Gotter A, Suppa C, Emanuel B. Mammalian TIMELESS and Tipin are evolutionarily conserved replication fork-associated factors. J Mol Biol. 2007;366:36-52 pubmed
  6. Prasad R, Williams J, Hou E, Wilson S. Pol ? associated complex and base excision repair factors in mouse fibroblasts. Nucleic Acids Res. 2012;40:11571-82 pubmed publisher
  7. Taniguchi J, Kondo K, Fujita K, Chen X, Homma H, Sudo T, et al. RpA1 ameliorates symptoms of mutant ataxin-1 knock-in mice and enhances DNA damage repair. Hum Mol Genet. 2016;25:4432-4447 pubmed publisher
    DNA damage and repair is a critical domain of many neurodegenerative diseases. In this study, we focused on RpA1, a candidate key molecule in polyQ disease pathologies, and tested the therapeutic effect of adeno-associated virus (AAV) ..
  8. Yang F, Gell K, van der Heijden G, Eckardt S, Leu N, Page D, et al. Meiotic failure in male mice lacking an X-linked factor. Genes Dev. 2008;22:682-91 pubmed publisher
    ..Thus, TEX11 promotes initiation and/or maintenance of synapsis and formation of crossovers, and may provide a physical link between these two meiotic processes. ..
  9. Yamane A, Resch W, Kuo N, Kuchen S, Li Z, Sun H, et al. Deep-sequencing identification of the genomic targets of the cytidine deaminase AID and its cofactor RPA in B lymphocytes. Nat Immunol. 2011;12:62-9 pubmed publisher
    ..In contrast, genomic occupancy of replication protein A (RPA), an AID cofactor, was restricted to immunoglobulin genes...
  10. Llano E, Herrán Y, García Tuñón I, Gutiérrez Caballero C, de Alava E, Barbero J, et al. Meiotic cohesin complexes are essential for the formation of the axial element in mice. J Cell Biol. 2012;197:877-85 pubmed publisher
  11. Banerjee P, Dejesus R, Gjoerup O, Schaffhausen B. Viral interference with DNA repair by targeting of the single-stranded DNA binding protein RPA. PLoS Pathog. 2013;9:e1003725 pubmed publisher
    ..the binding of its origin-binding domain (OBD) to the single-stranded DNA binding protein replication protein A (RPA). Overexpression of RPA protects cells expressing OBD from damage, and knockdown of RPA mimics the LT phenotype...
  12. Robert T, Nore A, Brun C, Maffre C, Crimi B, Bourbon H, et al. The TopoVIB-Like protein family is required for meiotic DNA double-strand break formation. Science. 2016;351:943-9 pubmed publisher
    ..We demonstrate that the mouse TOPOVIBL protein interacts and forms a complex with SPO11 and is required for meiotic DSB formation. We conclude that meiotic DSBs are catalyzed by a complex involving SPO11 and TOPOVIBL. ..
  13. Liu J, Kuo S, Melendy T. DNA damage-induced RPA focalization is independent of gamma-H2AX and RPA hyper-phosphorylation. J Cell Biochem. 2006;99:1452-62 pubmed
    Replication protein A (RPA) is the major eukaryotic single stranded DNA binding protein that plays a central role in DNA replication, repair and recombination...
  14. Kruger M, Ruschke K, Braun T. NSCL-1 and NSCL-2 synergistically determine the fate of GnRH-1 neurons and control necdin gene expression. EMBO J. 2004;23:4353-64 pubmed
    ..NSCL-dependent control of necdin expression might be instrumental for proper neuronal cell differentiation and enable GnRH-1 neurons to migrate. ..
  15. Kotani J, Avallone N, Lin E, Goshima M, Gandhi K, Lowry S, et al. Fas-mediated neutrophil apoptosis and associated A1 protein expression during systemic inflammation are regulated independently of both tumor necrosis factor receptors. Shock. 2003;19:201-7 pubmed
  16. Zhou C, Pourmal S, Pavletich N. Dna2 nuclease-helicase structure, mechanism and regulation by Rpa. elife. 2015;4: pubmed publisher
    ..Dna2 requires ssDNA ends, and is dependent on the ssDNA-binding protein Rpa, which controls cleavage polarity. Here we present the 2...
  17. Wang Y, Zhang W, Edelmann L, Kolodner R, Kucherlapati R, Edelmann W. Cis lethal genetic interactions attenuate and alter p53 tumorigenesis. Proc Natl Acad Sci U S A. 2010;107:5511-5 pubmed publisher
    b>Rpa1, an essential gene involved in DNA replication and genome maintenance, is syntenic and linked to Trp53 in mice and humans...
  18. Yamane A, Robbiani D, Resch W, Bothmer A, Nakahashi H, Oliveira T, et al. RPA accumulation during class switch recombination represents 5'-3' DNA-end resection during the S-G2/M phase of the cell cycle. Cell Rep. 2013;3:138-47 pubmed publisher
    ..b>RPA is a single-stranded DNA (ssDNA)-binding protein that associates with resected DSBs in the S phase and facilitates ..