Rex 1

Summary

Gene Symbol: Rex 1
Description: zinc finger protein 42
Alias: Rex-1, Rex1, Zfp-42, zinc finger protein 42, mREX-1, reduced expression protein 1
Species: mouse
Products:     Rex 1

Top Publications

  1. Hosler B, LaRosa G, Grippo J, Gudas L. Expression of REX-1, a gene containing zinc finger motifs, is rapidly reduced by retinoic acid in F9 teratocarcinoma cells. Mol Cell Biol. 1989;9:5623-9 pubmed
    ..The REX-1 gene is not identical to the previously reported murine genes that encode zinc finger-containing proteins. ..
  2. Rosfjord E, Rizzino A. The octamer motif present in the Rex-1 promoter binds Oct-1 and Oct-3 expressed by EC cells and ES cells. Biochem Biophys Res Commun. 1994;203:1795-802 pubmed
    ..In contrast, the other DNA/protein complexes, including the DNA/protein complex containing Oct-1, can still be detected. These results provide support for a role of Oct-3 in the transcription of the Rex-1 gene. ..
  3. Hosler B, Rogers M, Kozak C, Gudas L. An octamer motif contributes to the expression of the retinoic acid-regulated zinc finger gene Rex-1 (Zfp-42) in F9 teratocarcinoma cells. Mol Cell Biol. 1993;13:2919-28 pubmed
  4. Thompson J, Gudas L. Retinoic acid induces parietal endoderm but not primitive endoderm and visceral endoderm differentiation in F9 teratocarcinoma stem cells with a targeted deletion of the Rex-1 (Zfp-42) gene. Mol Cell Endocrinol. 2002;195:119-33 pubmed
    ..These results indicate that the Rex-1 transcription factor regulates the differentiation of F9 stem cells along several distinct cell lineages found in the early embryo...
  5. García Tuñón I, Guallar D, Alonso Martin S, Benito A, Benítez Lázaro A, Pérez Palacios R, et al. Association of Rex-1 to target genes supports its interaction with Polycomb function. Stem Cell Res. 2011;7:1-16 pubmed publisher
    ..Rex-1 might fine-tune pluripotency in ES cells by modulating Polycomb-mediated gene regulation. ..
  6. Borgel J, Guibert S, Li Y, Chiba H, Schübeler D, Sasaki H, et al. Targets and dynamics of promoter DNA methylation during early mouse development. Nat Genet. 2010;42:1093-100 pubmed publisher
    ..Finally, we identify nonimprinted genes that inherit promoter DNA methylation from parental gametes, suggesting that escape of post-fertilization DNA methylation reprogramming is prevalent in the mouse genome. ..
  7. Masui S, Ohtsuka S, Yagi R, Takahashi K, Ko M, Niwa H. Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells. BMC Dev Biol. 2008;8:45 pubmed publisher
    b>Rex1/Zfp42 has been extensively used as a marker for the undifferentiated state of pluripotent stem cells...
  8. Pelton T, Sharma S, Schulz T, Rathjen J, Rathjen P. Transient pluripotent cell populations during primitive ectoderm formation: correlation of in vivo and in vitro pluripotent cell development. J Cell Sci. 2002;115:329-39 pubmed
    ..Detailed mapping of these genes with Oct4, Rex1 and Fgf5 on pregastrulation embryos provided the first molecular evidence for the existence of successive, ..
  9. Rogers M, Hosler B, Gudas L. Specific expression of a retinoic acid-regulated, zinc-finger gene, Rex-1, in preimplantation embryos, trophoblast and spermatocytes. Development. 1991;113:815-24 pubmed
    ..These results suggest that Rex-1 is involved in trophoblast development and spermatogenesis, and is a useful marker for studies of early cell fate determination in the ICM. ..

Scientific Experts

More Information

Publications33

  1. Navarro P, Oldfield A, Legoupi J, Festuccia N, Dubois A, Attia M, et al. Molecular coupling of Tsix regulation and pluripotency. Nature. 2010;468:457-60 pubmed publisher
    ..Here we show that Tsix upregulation in embryonic stem cells depends on the recruitment of the pluripotent marker Rex1, and of the reprogramming-associated factors Klf4 and c-Myc, by the DXPas34 minisatellite associated with the Tsix ..
  2. Kim J, Faulk C, Kim J. Retroposition and evolution of the DNA-binding motifs of YY1, YY2 and REX1. Nucleic Acids Res. 2007;35:3442-52 pubmed
    ..one duplicate in flies, PHOL from PHO, and two duplicates in placental mammals, YY2 and Reduced Expression 1 (REX1) from YY1...
  3. Scotland K, Chen S, Sylvester R, Gudas L. Analysis of Rex1 (zfp42) function in embryonic stem cell differentiation. Dev Dyn. 2009;238:1863-77 pubmed publisher
    b>Rex1 (zfp42) is a zinc finger protein expressed primarily in undifferentiated stem cells, both in the embryo and the adult...
  4. Cormier J, zur Nieden N, Rancourt D, Kallos M. Expansion of undifferentiated murine embryonic stem cells as aggregates in suspension culture bioreactors. Tissue Eng. 2006;12:3233-45 pubmed
    ..Overcoming a major impediment in the area of ESC expansion, this study describes a successful process for the controlled and reproducible largescale expansion of ESCs using suspension culture bioreactors...
  5. Cook M, Munger S, Nadeau J, Capel B. Regulation of male germ cell cycle arrest and differentiation by DND1 is modulated by genetic background. Development. 2011;138:23-32 pubmed publisher
  6. Guallar D, Pérez Palacios R, Climent M, Martínez Abadía I, Larraga A, Fernández Juan M, et al. Expression of endogenous retroviruses is negatively regulated by the pluripotency marker Rex1/Zfp42. Nucleic Acids Res. 2012;40:8993-9007 pubmed publisher
    b>Rex1/Zfp42 is a Yy1-related zinc-finger protein whose expression is frequently used to identify pluripotent stem cells...
  7. Nishioka N, Yamamoto S, Kiyonari H, Sato H, Sawada A, Ota M, et al. Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos. Mech Dev. 2008;125:270-83 pubmed
  8. Xu J, Sylvester R, Tighe A, Chen S, Gudas L. Transcriptional activation of the suppressor of cytokine signaling-3 (SOCS-3) gene via STAT3 is increased in F9 REX1 (ZFP-42) knockout teratocarcinoma stem cells relative to wild-type cells. J Mol Biol. 2008;377:28-46 pubmed publisher
    b>Rex1 (Zfp42), first identified as a gene that is transcriptionally repressed by retinoic acid (RA), encodes a zinc finger transcription factor expressed at high levels in F9 teratocarcinoma stem cells, embryonic stem cells, and other stem ..
  9. Climent M, Alonso Martin S, Pérez Palacios R, Guallar D, Benito A, Larraga A, et al. Functional analysis of Rex1 during preimplantation development. Stem Cells Dev. 2013;22:459-72 pubmed publisher
    b>Rex1/Zfp42 is a nuclear protein that is highly conserved in mammals, and widely used as an embryonic stem (ES) cell marker...
  10. Han D, Tapia N, Joo J, Greber B, Arauzo Bravo M, Bernemann C, et al. Epiblast stem cell subpopulations represent mouse embryos of distinct pregastrulation stages. Cell. 2010;143:617-27 pubmed publisher
    ..Our findings suggest that the rare ability of EpiSCs to contribute to chimeras is due to the presence of the minor EpiSC fraction representing the early epiblast. ..
  11. Son M, Choi H, Han Y, Cho Y. Unveiling the critical role of REX1 in the regulation of human stem cell pluripotency. Stem Cells. 2013;31:2374-87 pubmed publisher
    Reduced expression 1 (REX1) is a widely used pluripotency marker, but little is known about its roles in pluripotency. Here, we show that REX1 is functionally important in the reacquisition and maintenance of pluripotency...
  12. Hamatani T, Carter M, Sharov A, Ko M. Dynamics of global gene expression changes during mouse preimplantation development. Dev Cell. 2004;6:117-31 pubmed
    ..The large number of genes identified as involved in each phase is a first step toward analysis of the complex gene regulatory networks. ..
  13. Oda M, Oxley D, Dean W, Reik W. Regulation of lineage specific DNA hypomethylation in mouse trophectoderm. PLoS ONE. 2013;8:e68846 pubmed publisher
    ..This resistance may be based on transcription factors or on global differences in chromatin structure. ..
  14. Wu G, Han D, Gong Y, Sebastiano V, Gentile L, Singhal N, et al. Establishment of totipotency does not depend on Oct4A. Nat Cell Biol. 2013;15:1089-97 pubmed publisher
  15. Nakai Futatsugi Y, Niwa H. Zscan4 Is Activated after Telomere Shortening in Mouse Embryonic Stem Cells. Stem Cell Reports. 2016;6:483-495 pubmed publisher
    ..Using live cell imaging, we monitored the activity of Zscan4 together with the pluripotency marker Rex1. The lengths of the cell cycles in ESCs were diverse...
  16. Rieger C, Poppino R, Sheridan R, Moley K, Mitra R, Gottlieb D. Polony analysis of gene expression in ES cells and blastocysts. Nucleic Acids Res. 2007;35:e151 pubmed
    ..Genes assayed include Oct3, Rex1, Nanog, Cdx2 and GLUT-1...
  17. Das P, Doyle T, Liu D, Kochar J, Kim K, Rogers M. Retinoic acid regulation of eye and testis-specific transcripts within a complex locus. Mech Dev. 2007;124:137-45 pubmed
    ..Furthermore, our analyses of transcript levels in eyes and testes isolated from vitamin A deficient mice or from mice with defects in retinoid storage or signaling indicate that retinoids are required for expression in vivo. ..
  18. Lamoury F, Croitoru Lamoury J, Brew B. Undifferentiated mouse mesenchymal stem cells spontaneously express neural and stem cell markers Oct-4 and Rex-1. Cytotherapy. 2006;8:228-42 pubmed
    ..These results should facilitate the optimal timing of harvesting a neurally fated subpopulation of mMSC for transplantation into animal models of human brain diseases. ..
  19. Gratsch T, O Shea K. Noggin and chordin have distinct activities in promoting lineage commitment of mouse embryonic stem (ES) cells. Dev Biol. 2002;245:83-94 pubmed
    ..These data suggest that, consistent with their different expression fields, noggin and chordin may play distinct roles in patterning the early mouse embryo. ..
  20. Miyanari Y, Torres Padilla M. Control of ground-state pluripotency by allelic regulation of Nanog. Nature. 2012;483:470-3 pubmed publisher
    ..Our data highlight an unexpected role for allelic expression in controlling the dose of pluripotency factors in vivo, adding an extra level to the regulation of reprogramming. ..
  21. Gontan C, Achame E, Demmers J, Barakat T, Rentmeester E, van Ijcken W, et al. RNF12 initiates X-chromosome inactivation by targeting REX1 for degradation. Nature. 2012;485:386-90 pubmed publisher
    ..Here we identify the pluripotency factor REX1 as a key target of RNF12 in the mechanism of X-chromosome inactivation...
  22. Rezende N, Lee M, Monette S, Mark W, Lu A, Gudas L. Rex1 (Zfp42) null mice show impaired testicular function, abnormal testis morphology, and aberrant gene expression. Dev Biol. 2011;356:370-82 pubmed publisher
    b>Rex1 (Zfp42), GeneID 132625, is a gene whose expression is closely associated with pluripotency/multipotency in both mouse and human embryonic stem cells...
  23. van der Stoop P, Boutsma E, Hulsman D, Noback S, Heimerikx M, Kerkhoven R, et al. Ubiquitin E3 ligase Ring1b/Rnf2 of polycomb repressive complex 1 contributes to stable maintenance of mouse embryonic stem cells. PLoS ONE. 2008;3:e2235 pubmed publisher
    ..These genes are characterized by high CpG content promoters and bivalent histone marks or the active H3K4me3 histone mark alone. ..
  24. Alexandrova S, Kalkan T, Humphreys P, Riddell A, Scognamiglio R, Trumpp A, et al. Selection and dynamics of embryonic stem cell integration into early mouse embryos. Development. 2016;143:24-34 pubmed publisher
    ..Using a fluorescent reporter for naive pluripotency (Rex1-GFP), we established that the acutely eliminated serum/LIF ESCs had started to differentiate...