Gene Symbol: Rev1
Description: REV1, DNA directed polymerase
Alias: 1110027I23Rik, AU022044, Rev1l, DNA repair protein REV1, REV1 homolog, rev1-like terminal deoxycytidyl transferase
Species: mouse
Products:     Rev1

Top Publications

  1. Guo C, Fischhaber P, Luk Paszyc M, Masuda Y, Zhou J, Kamiya K, et al. Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis. EMBO J. 2003;22:6621-30 pubmed
    Pol kappa and Rev1 are members of the Y family of DNA polymerases involved in tolerance to DNA damage by replicative bypass [translesion DNA synthesis (TLS)]. We demonstrate that mouse Rev1 protein physically associates with Pol kappa...
  2. Masuda Y, Takahashi M, Fukuda S, Sumii M, Kamiya K. Mechanisms of dCMP transferase reactions catalyzed by mouse Rev1 protein. J Biol Chem. 2002;277:3040-6 pubmed
    The Rev1 protein, a member of a large family of translesion DNA polymerases, catalyzes a dCMP transfer reaction...
  3. Guo C, Tang T, Bienko M, Parker J, Bielen A, Sonoda E, et al. Ubiquitin-binding motifs in REV1 protein are required for its role in the tolerance of DNA damage. Mol Cell Biol. 2006;26:8892-900 pubmed
    b>REV1 protein is a eukaryotic member of the Y family of DNA polymerases involved in the tolerance of DNA damage by replicative bypass. The precise role(s) of REV1 in this process is not known...
  4. Jansen J, Tsaalbi Shtylik A, Langerak P, Calléja F, Meijers C, Jacobs H, et al. The BRCT domain of mammalian Rev1 is involved in regulating DNA translesion synthesis. Nucleic Acids Res. 2005;33:356-65 pubmed
    b>Rev1 is a deoxycytidyl transferase associated with DNA translesion synthesis (TLS). In addition to its catalytic domain, Rev1 possesses a so-called BRCA1 C-terminal (BRCT) domain...
  5. Jansen J, Langerak P, Tsaalbi Shtylik A, van den Berk P, Jacobs H, de Wind N. Strand-biased defect in C/G transversions in hypermutating immunoglobulin genes in Rev1-deficient mice. J Exp Med. 2006;203:319-23 pubmed
    ..b>Rev1 is a translesion synthesis polymerase that in vitro incorporates uniquely deoxycytidine opposite deoxyuridine and ..
  6. Shen X, Jun S, O Neal L, Sonoda E, Bemark M, Sale J, et al. REV3 and REV1 play major roles in recombination-independent repair of DNA interstrand cross-links mediated by monoubiquitinated proliferating cell nuclear antigen (PCNA). J Biol Chem. 2006;281:13869-72 pubmed
    ..of lesion bypass polymerases in ICL repair, we investigated recombination-independent repair of ICLs in REV3 and REV1 deletion mutants constructed in avian DT40 cells and mouse embryonic fibroblast cells...
  7. Guo C, Sonoda E, Tang T, Parker J, Bielen A, Takeda S, et al. REV1 protein interacts with PCNA: significance of the REV1 BRCT domain in vitro and in vivo. Mol Cell. 2006;23:265-71 pubmed
    b>REV1 protein, a eukaryotic member of the Y family of DNA polymerases, is involved in the tolerance of DNA damage by translesion DNA synthesis. It is unclear how REV1 is recruited to replication foci in cells...
  8. Dumstorf C, Mukhopadhyay S, Krishnan E, Haribabu B, McGregor W. REV1 is implicated in the development of carcinogen-induced lung cancer. Mol Cancer Res. 2009;7:247-54 pubmed publisher
    ..The expression of this protein, REV1, was reduced in mouse cells using a vector encoding a gene-specific targeting ribozyme...
  9. Masuda K, Ouchida R, Li Y, Gao X, Mori H, Wang J. A critical role for REV1 in regulating the induction of C:G transitions and A:T mutations during Ig gene hypermutation. J Immunol. 2009;183:1846-50 pubmed publisher
    b>REV1 is a deoxycytidyl transferase that catalyzes the incorporation of deoxycytidines opposite deoxyguanines and abasic sites...

More Information


  1. Jansen J, Tsaalbi Shtylik A, Hendriks G, Gali H, Hendel A, Johansson F, et al. Separate domains of Rev1 mediate two modes of DNA damage bypass in mammalian cells. Mol Cell Biol. 2009;29:3113-23 pubmed publisher
    The Y family DNA polymerase Rev1 has been proposed to play a regulatory role in the replication of damaged templates...
  2. Kano C, Hanaoka F, Wang J. Analysis of mice deficient in both REV1 catalytic activity and POLH reveals an unexpected role for POLH in the generation of C to G and G to C transversions during Ig gene hypermutation. Int Immunol. 2012;24:169-74 pubmed publisher
    ..Mice expressing a catalytically inactive REV1 (REV1AA) exhibit reduction of both C to G and G to C transversions and moderate decrease of A/T mutations, whereas ..
  3. Enoiu M, Jiricny J, SCHARER O. Repair of cisplatin-induced DNA interstrand crosslinks by a replication-independent pathway involving transcription-coupled repair and translesion synthesis. Nucleic Acids Res. 2012;40:8953-64 pubmed publisher
    ..In this pathway, Rev1 and Pol? are involved in the error-free bypass of cisplatin ICLs...
  4. Hashimoto K, Cho Y, Yang I, Akagi J, Ohashi E, Tateishi S, et al. The vital role of polymerase ? and REV1 in mutagenic, but not correct, DNA synthesis across benzo[a]pyrene-dG and recruitment of polymerase ? by REV1 to replication-stalled site. J Biol Chem. 2012;287:9613-22 pubmed publisher
    ..Knockout of the Rev1 gene decreased both the TLS efficiency and the miscoding frequency...
  5. Wojtaszek J, Liu J, D Souza S, Wang S, Xue Y, Walker G, et al. Multifaceted recognition of vertebrate Rev1 by translesion polymerases ? and ?. J Biol Chem. 2012;287:26400-8 pubmed publisher
    ..The eukaryotic Y-family polymerase Rev1 is an essential scaffolding protein in translesion synthesis...
  6. Kim H, Yang K, Dejsuphong D, D Andrea A. Regulation of Rev1 by the Fanconi anemia core complex. Nat Struct Mol Biol. 2012;19:164-70 pubmed publisher
    ..Recent studies indicate that the Fanconi anemia pathway also controls Rev1-mediated translesion DNA synthesis (TLS)...
  7. Wang Z, Huang M, Ma X, Li H, Tang T, Guo C. REV1 promotes PCNA monoubiquitylation through interacting with ubiquitylated RAD18. J Cell Sci. 2016;129:1223-33 pubmed publisher
    ..So far, it remains unclear how the TLS pathway is elaborately regulated. Here, we report that TLS polymerase REV1 can promote PCNA monoubiquitylation after UV radiation...
  8. Krijger P, Tsaalbi Shtylik A, Wit N, van den Berk P, de Wind N, Jacobs H. Rev1 is essential in generating G to C transversions downstream of the Ung2 pathway but not the Msh2+Ung2 hybrid pathway. Eur J Immunol. 2013;43:2765-70 pubmed publisher
    ..Besides Ung2, the mismatch repair protein Msh2 and the translesion synthesis (TLS) DNA polymerase (Pol) Rev1 are implicated in SHM and CSR...
  9. Prasad R, Poltoratsky V, Hou E, Wilson S. Rev1 is a base excision repair enzyme with 5'-deoxyribose phosphate lyase activity. Nucleic Acids Res. 2016;44:10824-10833 pubmed
    b>Rev1 is a member of the Y-family of DNA polymerases and is known for its deoxycytidyl transferase activity that incorporates dCMP into DNA and its ability to function as a scaffold factor for other Y-family polymerases in translesion ..
  10. Ito W, Yokoi M, Sakayoshi N, Sakurai Y, Akagi J, Mitani H, et al. Stalled Pol? at its cognate substrate initiates an alternative translesion synthesis pathway via interaction with REV1. Genes Cells. 2012;17:98-108 pubmed publisher
    ..As Pol? interacts with REV1, and REV1 interacts with other TLS polymerases including Pol?, Pol? and Pol?, Pol? may play a role in recruitment ..
  11. Tsaalbi Shtylik A, Verspuy J, Jansen J, Rebel H, Carlée L, van der Valk M, et al. Error-prone translesion replication of damaged DNA suppresses skin carcinogenesis by controlling inflammatory hyperplasia. Proc Natl Acad Sci U S A. 2009;106:21836-41 pubmed publisher
    ..nucleotide excision repair-deficient mouse model that carries a hypomorphic allele of the error-prone TLS gene Rev1. Despite a defect in UV-induced mutagenesis, skin carcinogenesis was accelerated in these mice...
  12. Ohashi E, Hanafusa T, Kamei K, Song I, Tomida J, Hashimoto H, et al. Identification of a novel REV1-interacting motif necessary for DNA polymerase kappa function. Genes Cells. 2009;14:101-11 pubmed publisher
    ..cells have several TLS DNA polymerases, including the four Y-family enzymes (Poleta, Poliota, Polkappa and REV1) that share multiple primary sequence motifs, but show preferential bypass of different DNA lesions...
  13. Lv L, Wang F, Ma X, Yang Y, Wang Z, Liu H, et al. Mismatch repair protein MSH2 regulates translesion DNA synthesis following exposure of cells to UV radiation. Nucleic Acids Res. 2013;41:10312-22 pubmed publisher
    ..Interestingly, expression of MSH2 in Rad18-deficient cells increased UV-induced Pol? and REV1 focus formation without detectable changes in PCNA monoubiquitination, indicating that MSH2 can regulate post-UV ..
  14. Shim H, Wei M, Brandhorst S, Longo V. Starvation promotes REV1 SUMOylation and p53-dependent sensitization of melanoma and breast cancer cells. Cancer Res. 2015;75:1056-67 pubmed publisher
    ..Here, we describe the role of REV1, a specialized DNA polymerase involved in DNA repair, as an important signaling node linking nutrient sensing and ..
  15. Yoon J, Park J, Conde J, Wakamiya M, Prakash L, Prakash S. Rev1 promotes replication through UV lesions in conjunction with DNA polymerases η, ι, and κ but not DNA polymerase ζ. Genes Dev. 2015;29:2588-602 pubmed publisher
    ..In yeast, Rev1 plays a noncatalytic role as an indispensable component of Polζ, and Polζ together with Rev1 mediates a ..
  16. Poltoratsky V, Horton J, Prasad R, Wilson S. REV1 mediated mutagenesis in base excision repair deficient mouse fibroblast. DNA Repair (Amst). 2005;4:1182-8 pubmed
    ..We studied the effect of RNAi mediated down-regulation of the lesion bypass factor REV1. The steady-state level of REV1 protein was reduced by more than 95% using stable expression of a siRNA construct ..
  17. Wojtaszek J, Lee C, D Souza S, Minesinger B, Kim H, D Andrea A, et al. Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric polymerase (Pol) ?, and Pol ?. J Biol Chem. 2012;287:33836-46 pubmed
    ..synthesis in mammalian cells is achieved in two sequential steps, with a Y-family DNA polymerase (?, ?, ?, or Rev1) inserting a nucleotide opposite the lesion and with the heterodimeric B-family polymerase ?, consisting of the ..
  18. Zan H, White C, Thomas L, Mai T, Li G, Xu Z, et al. Rev1 recruits ung to switch regions and enhances du glycosylation for immunoglobulin class switch DNA recombination. Cell Rep. 2012;2:1220-32 pubmed publisher
    ..Here we showed that the translesion DNA synthesis polymerase Rev1 directly interacted with Ung and targeted in an AID-dependent and Ung-independent fashion the S regions undergoing ..
  19. Oberbeck N, Langevin F, King G, de Wind N, Crossan G, Patel K. Maternal aldehyde elimination during pregnancy preserves the fetal genome. Mol Cell. 2014;55:807-817 pubmed publisher
  20. Hashimoto K, Bonala R, Johnson F, Grollman A, Moriya M. Y-family DNA polymerase-independent gap-filling translesion synthesis across aristolochic acid-derived adenine adducts in mouse cells. DNA Repair (Amst). 2016;46:55-60 pubmed publisher
    ..Similarly, knockout of the Rev1 gene did not significantly affect TLS...
  21. Sasatani M, Xi Y, Kajimura J, Kawamura T, Piao J, Masuda Y, et al. Overexpression of Rev1 promotes the development of carcinogen-induced intestinal adenomas via accumulation of point mutation and suppression of apoptosis proportionally to the Rev1 expression level. Carcinogenesis. 2017;38:570-578 pubmed publisher
    ..Aberrant activation of this pathway plays a role in tumorigenesis by promoting genetic mutations. Rev1 controls the function of the TLS pathway, and Rev1 expression levels are associated with DNA damage induced ..