Rec8

Summary

Gene Symbol: Rec8
Description: REC8 meiotic recombination protein
Alias: Rec8L1, mrec, meiotic recombination protein REC8 homolog, REC8-like 1, cohesin Rec8p, meiotic cohesion Rec8
Species: mouse
Products:     Rec8

Top Publications

  1. Parisi S, McKay M, Molnar M, Thompson M, van der Spek P, van Drunen Schoenmaker E, et al. Rec8p, a meiotic recombination and sister chromatid cohesion phosphoprotein of the Rad21p family conserved from fission yeast to humans. Mol Cell Biol. 1999;19:3515-28 pubmed
    Our work and that of others defined mitosis-specific (Rad21 subfamily) and meiosis-specific (Rec8 subfamily) proteins involved in sister chromatid cohesion in several eukaryotes, including humans...
  2. Baltus A, Menke D, Hu Y, Goodheart M, Carpenter A, de Rooij D, et al. In germ cells of mouse embryonic ovaries, the decision to enter meiosis precedes premeiotic DNA replication. Nat Genet. 2006;38:1430-4 pubmed
    ..Combined with previous findings, these genetic data suggest that active differentiation of ovarian germ cells commences at a regulatory point upstream of premeiotic DNA replication. ..
  3. Lee J, Hirano T. RAD21L, a novel cohesin subunit implicated in linking homologous chromosomes in mammalian meiosis. J Cell Biol. 2011;192:263-76 pubmed publisher
    ..RAD21L then disappears, and is replaced with RAD21. This behavior of RAD21L is unique and distinct from that of REC8, another meiosis-specific kleisin subunit...
  4. Lee J, Iwai T, Yokota T, Yamashita M. Temporally and spatially selective loss of Rec8 protein from meiotic chromosomes during mammalian meiosis. J Cell Sci. 2003;116:2781-90 pubmed publisher
    ..We report here the first evidence of the involvement of Rec8 protein, a mammalian homolog of yeast Rec8p, in meiosis-specific chromosome behavior in mammals...
  5. Jordan P, Karppinen J, Handel M. Polo-like kinase is required for synaptonemal complex disassembly and phosphorylation in mouse spermatocytes. J Cell Sci. 2012;125:5061-72 pubmed publisher
    ..These findings provide mechanistic details of the first stage of SC disassembly in mammalian spermatocytes, and reveal that PLK-mediated phosphorylation of central element proteins is required for meiotic prophase exit...
  6. Kouznetsova A, Novak I, Jessberger R, Hoog C. SYCP2 and SYCP3 are required for cohesin core integrity at diplotene but not for centromere cohesion at the first meiotic division. J Cell Sci. 2005;118:2271-8 pubmed
    ..This finding supports a model in which the removal of bulk cohesin from paired sister chromatids at late prophase in both meiotic and mitotic cells ensures proper chromosome compaction and segregation. ..
  7. Novak I, Wang H, Revenkova E, Jessberger R, Scherthan H, Hoog C. Cohesin Smc1beta determines meiotic chromatin axis loop organization. J Cell Biol. 2008;180:83-90 pubmed publisher
    ..Our results suggest that in addition to its role in sister chromatid cohesion, Smc1beta determines meiotic chromatin loop organization. ..
  8. Bannister L, Reinholdt L, Munroe R, Schimenti J. Positional cloning and characterization of mouse mei8, a disrupted allelle of the meiotic cohesin Rec8. Genesis. 2004;40:184-94 pubmed
    ..Genetic mapping and positional cloning of mei8 led to the identification of a mutation in Rec8, a homolog of the yeast meiosis-specific cohesin gene REC8...
  9. Xu H, Beasley M, Warren W, van der Horst G, McKay M. Absence of mouse REC8 cohesin promotes synapsis of sister chromatids in meiosis. Dev Cell. 2005;8:949-61 pubmed
    b>REC8 is a key component of the meiotic cohesin complex...

More Information

Publications55

  1. Li X, Li X, Schimenti J. Mouse pachytene checkpoint 2 (trip13) is required for completing meiotic recombination but not synapsis. PLoS Genet. 2007;3:e130 pubmed
    ..Double mutant analysis demonstrated that the recombination and synapsis genes Spo11, Mei1, Rec8, and Dmc1 are all epistatic to Trip13, suggesting that TRIP13 does not have meiotic checkpoint function in mice...
  2. Prieto I, Pezzi N, Buesa J, Kremer L, Barthelemy I, Carreiro C, et al. STAG2 and Rad21 mammalian mitotic cohesins are implicated in meiosis. EMBO Rep. 2002;3:543-50 pubmed
    ..Immunofluorescence results suggest that a cohesin complex containing Rad21 and STAG2 cooperates with a STAG3-specific complex to maintain sister chromatid cohesion during the diplotene stage of meiosis. ..
  3. Llano E, Herrán Y, García Tuñón I, Gutiérrez Caballero C, de Alava E, Barbero J, et al. Meiotic cohesin complexes are essential for the formation of the axial element in mice. J Cell Biol. 2012;197:877-85 pubmed publisher
    ..Yeast, but not mice, depleted of the cohesin subunit Rec8 are defective in the formation of the axial elements (AEs) of the SC, suggesting that, in mammals, this function is ..
  4. Murdoch B, Owen N, Stevense M, Smith H, Nagaoka S, Hassold T, et al. Altered cohesin gene dosage affects Mammalian meiotic chromosome structure and behavior. PLoS Genet. 2013;9:e1003241 pubmed publisher
    ..We report here evidence from the mouse that partial loss of gene function for either Smc1b or Rec8 causes perturbations in the formation of the synaptonemal complex (SC) and affects both synapsis and recombination ..
  5. Lee J, Kitajima T, Tanno Y, Yoshida K, Morita T, Miyano T, et al. Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells. Nat Cell Biol. 2008;10:42-52 pubmed
    ..in germ cells and is solely responsible for the centromeric localization of PP2A and the protection of cohesin Rec8 in oocytes, proving conservation of the mechanism from yeast to mammals...
  6. Ishiguro K, Kim J, Shibuya H, Hern ndez Hern ndez A, Suzuki A, Fukagawa T, et al. Meiosis-specific cohesin mediates homolog recognition in mouse spermatocytes. Genes Dev. 2014;28:594-607 pubmed publisher
    ..These findings suggest the intriguing possibility that homolog recognition is achieved primarily by searching for homology in the chromosome architecture as defined by meiosis-specific cohesin rather than in the DNA sequence itself...
  7. Llano E, Gómez R, Gutiérrez Caballero C, Herrán Y, Sanchez Martin M, Vázquez Quiñones L, et al. Shugoshin-2 is essential for the completion of meiosis but not for mitotic cell division in mice. Genes Dev. 2008;22:2400-13 pubmed publisher
    ..In vivo, the loss of SGOL2 promotes a premature release of the meiosis-specific REC8 cohesin complexes from anaphase I centromeres...
  8. Lin Y, Gill M, Koubova J, Page D. Germ cell-intrinsic and -extrinsic factors govern meiotic initiation in mouse embryos. Science. 2008;322:1685-7 pubmed publisher
    ..Within a brief developmental window, Dazl-expressing germ cells in both XX and XY embryos actively acquire the ability to interpret RA as a meiosis-inducing signal. ..
  9. Lee J, Okada K, Ogushi S, Miyano T, Miyake M, Yamashita M. Loss of Rec8 from chromosome arm and centromere region is required for homologous chromosome separation and sister chromatid separation, respectively, in mammalian meiosis. Cell Cycle. 2006;5:1448-55 pubmed
    ..We show here that meiosis-specific cohesin subunit Rec8 in mouse oocytes shows essentially the same pattern of localization to those reported in yeasts and mammalian ..
  10. Shibuya H, Ishiguro K, Watanabe Y. The TRF1-binding protein TERB1 promotes chromosome movement and telomere rigidity in meiosis. Nat Cell Biol. 2014;16:145-56 pubmed publisher
    ..Our study suggests that TERB1 acts as a central hub for the assembly of a conserved meiotic telomere complex required for chromosome movements. ..
  11. Lee J, Yokota T, Yamashita M. Analyses of mRNA expression patterns of cohesin subunits Rad21 and Rec8 in mice: germ cell-specific expression of rec8 mRNA in both male and female mice. Zoolog Sci. 2002;19:539-44 pubmed
    ..here the mRNA expression patterns of mouse homologues of the yeast mitotic cohesin rad21 and the meiotic cohesin rec8 in various organs, with special attention to their expression in gonads...
  12. Tachibana Konwalski K, Godwin J, van der Weyden L, Champion L, Kudo N, Adams D, et al. Rec8-containing cohesin maintains bivalents without turnover during the growing phase of mouse oocytes. Genes Dev. 2010;24:2505-16 pubmed publisher
    ..by sister chromatid cohesion mediated by cohesin complexes whose ring structure depends on kleisin subunits, either Rec8 or Scc1...
  13. Kouznetsova A, Benavente R, Pastink A, Hoog C. Meiosis in mice without a synaptonemal complex. PLoS ONE. 2011;6:e28255 pubmed publisher
  14. Herrán Y, Gutiérrez Caballero C, Sanchez Martin M, Hernandez T, Viera A, Barbero J, et al. The cohesin subunit RAD21L functions in meiotic synapsis and exhibits sexual dimorphism in fertility. EMBO J. 2011;30:3091-105 pubmed publisher
    ..onset of anaphase when the ring is opened by proteolytic cleavage of its ?-kleisin subunit (RAD21 at mitosis and REC8 at meiosis) by separase...
  15. Agostinho A, Manneberg O, van Schendel R, Hernández Hernández A, Kouznetsova A, Blom H, et al. High density of REC8 constrains sister chromatid axes and prevents illegitimate synaptonemal complex formation. EMBO Rep. 2016;17:901-13 pubmed publisher
    ..We show that REC8 foci are separated from each other by a distance smaller than 15% of the total chromosome axis length in wild-type ..
  16. Chiang T, Schultz R, Lampson M. Age-dependent susceptibility of chromosome cohesion to premature separase activation in mouse oocytes. Biol Reprod. 2011;85:1279-83 pubmed publisher
    ..To directly target cohesion, we increased the activity of separase, the protease that cleaves the meiotic cohesin REC8, in oocytes...
  17. Jordan P, Eyster C, Chen J, Pezza R, Rankin S. Sororin is enriched at the central region of synapsed meiotic chromosomes. Chromosome Res. 2017;25:115-128 pubmed publisher
    ..These results indicate that Sororin regulation during meiosis is distinct from its regulation in mitotic cells and may suggest that it interacts with a distinctly different partner to ensure proper chromosome dynamics in meiosis. ..
  18. Hopkins J, Hwang G, Jacob J, Sapp N, Bedigian R, Oka K, et al. Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes. PLoS Genet. 2014;10:e1004413 pubmed publisher
    ..Previous studies in mouse have shown that there is one SMC1 protein (SMC1?), two ?-kleisins (RAD21L and REC8) and one STAG protein (STAG3) that are meiosis-specific...
  19. Shin Y, Ren Y, Suzuki H, Golnoski K, Ahn H, Mico V, et al. Transcription factors SOHLH1 and SOHLH2 coordinate oocyte differentiation without affecting meiosis I. J Clin Invest. 2017;127:2106-2117 pubmed publisher
    ..Our results indicate that Sohlh1 and Sohlh2 are essential regulators of oocyte differentiation but do not affect meiosis I. ..
  20. Huo L, Zhong Z, Liang C, Wang Q, Yin S, Ai J, et al. Degradation of securin in mouse and pig oocytes is dependent on ubiquitin-proteasome pathway and is required for proteolysis of the cohesion subunit, Rec8, at the metaphase-to-anaphase transition. Front Biosci. 2006;11:2193-202 pubmed
    ..In the present study, we investigated the quantity of securin and Rec8 protein and the localization of securin, a cohesion subunit, during oocyte meiosis providing data in support of the ..
  21. Hwang G, Sun F, O Brien M, Eppig J, Handel M, Jordan P. SMC5/6 is required for the formation of segregation-competent bivalent chromosomes during meiosis I in mouse oocytes. Development. 2017;144:1648-1660 pubmed publisher
  22. Kudo N, Anger M, Peters A, Stemmann O, Theussl H, Helmhart W, et al. Role of cleavage by separase of the Rec8 kleisin subunit of cohesin during mammalian meiosis I. J Cell Sci. 2009;122:2686-98 pubmed publisher
    Proteolytic activity of separase is required for chiasma resolution during meiosis I in mouse oocytes. Rec8, the meiosis-specific alpha-kleisin subunit of cohesin, is a key target of separase in yeast...
  23. Soh Y, Junker J, Gill M, Mueller J, van Oudenaarden A, Page D. A Gene Regulatory Program for Meiotic Prophase in the Fetal Ovary. PLoS Genet. 2015;11:e1005531 pubmed publisher
    ..Unexpectedly, we discovered that Stra8 is required for prompt down-regulation of itself and Rec8. Germ cells that have expressed and down-regulated Stra8 are refractory to further Stra8 expression...
  24. Fukuda T, Fukuda N, Agostinho A, Hernández Hernández A, Kouznetsova A, Hoog C. STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis. EMBO J. 2014;33:1243-55 pubmed publisher
    ..find that the three different ?-kleisins present in meiotic cells show different dosage-dependent requirements for STAG3 and that STAG3-REC8 cohesin complexes have a critical role in supporting meiotic chromosome structure and functions.
  25. Liu Y, Liu C, Chang Z, Wadas B, Brower C, Song Z, et al. Degradation of the Separase-cleaved Rec8, a Meiotic Cohesin Subunit, by the N-end Rule Pathway. J Biol Chem. 2016;291:7426-38 pubmed publisher
    ..In contrast, the separase-mediated cleavage of Rec8, the mammalian meiotic cohesin subunit, yields a fragment bearing N-terminal Glu, a substrate of the Ate1 R-..
  26. Biswas U, Hempel K, Llano E, Pendas A, Jessberger R. Distinct Roles of Meiosis-Specific Cohesin Complexes in Mammalian Spermatogenesis. PLoS Genet. 2016;12:e1006389 pubmed publisher
    ..To decipher the functions of the two meiosis-specific kleisins, REC8 or RAD21L, together with the only meiosis-specific SMC protein SMC1?, we generated Smc1?-/-Rec8-/- and Smc1?-/-..
  27. Soh Y, Mikedis M, Kojima M, Godfrey A, de Rooij D, Page D. Meioc maintains an extended meiotic prophase I in mice. PLoS Genet. 2017;13:e1006704 pubmed publisher
    ..Specifically, MEIOC, together with YTHDC2, promotes a meiotic (as opposed to mitotic) cell cycle program via post-transcriptional control of their target transcripts. ..
  28. Wang R, Payseur B. Genetics of Genome-Wide Recombination Rate Evolution in Mice from an Isolated Island. Genetics. 2017;206:1841-1852 pubmed publisher
    ..Combined with existing studies, our findings suggest that genome-wide recombination rate divergence is not directional and its evolution within and between subspecies proceeds from distinct genetic loci. ..
  29. Gómez R, Jordan P, Viera A, Alsheimer M, Fukuda T, Jessberger R, et al. Dynamic localization of SMC5/6 complex proteins during mammalian meiosis and mitosis suggests functions in distinct chromosome processes. J Cell Sci. 2013;126:4239-52 pubmed publisher
  30. Strauss T, Castrillon D, Hammes S. GATA-like protein-1 (GLP-1) is required for normal germ cell development during embryonic oogenesis. Reproduction. 2011;141:173-81 pubmed publisher
    ..Together, these data imply that the somatic cell protein GLP-1 is not necessary for many pregranulosa cell functions but is required for germ cell survival. ..
  31. Koubova J, Hu Y, Bhattacharyya T, Soh Y, Gill M, Goodheart M, et al. Retinoic acid activates two pathways required for meiosis in mice. PLoS Genet. 2014;10:e1004541 pubmed publisher
    ..Here we report that RA also activates transcription of Rec8, which encodes a component of the cohesin complex that accumulates during meiotic S phase, and which is essential ..
  32. Du J, Cao Y, Wang Q, Zhang N, Liu X, Chen D, et al. Unique subcellular distribution of phosphorylated Plk1 (Ser137 and Thr210) in mouse oocytes during meiotic division and pPlk1(Ser137) involvement in spindle formation and REC8 cleavage. Cell Cycle. 2015;14:3566-79 pubmed publisher
    ..disturbed γ-tubulin and pericentrin recruiting to MTOCs, destroyed meiotic spindle formation, and delayed REC8 cleavage, therefore arresting oocytes at metaphase I (MI) with chromosome misalignment...
  33. Shum E, Jones S, Shao A, Dumdie J, Krause M, Chan W, et al. The Antagonistic Gene Paralogs Upf3a and Upf3b Govern Nonsense-Mediated RNA Decay. Cell. 2016;165:382-95 pubmed publisher
    ..Mice conditionally lacking UPF3A exhibit "hyper" NMD and display defects in embryogenesis and gametogenesis. Our results support a model in which UPF3A serves as a molecular rheostat that directs developmental events. ..
  34. Bailey A, Batista P, Gold R, Chen Y, de Rooij D, Chang H, et al. The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline. elife. 2017;6: pubmed publisher
  35. Lee J, Ogushi S, Saitou M, Hirano T. Condensins I and II are essential for construction of bivalent chromosomes in mouse oocytes. Mol Biol Cell. 2011;22:3465-77 pubmed publisher
    ..b>REC8 "glues" chromosome arms along their lengths...
  36. Kudo N, Wassmann K, Anger M, Schuh M, Wirth K, Xu H, et al. Resolution of chiasmata in oocytes requires separase-mediated proteolysis. Cell. 2006;126:135-46 pubmed
    In yeast, resolution of chiasmata in meiosis I requires proteolytic cleavage along chromosome arms of cohesin's Rec8 subunit by separase...
  37. Seitan V, Faure A, Zhan Y, McCord R, Lajoie B, Ing Simmons E, et al. Cohesin-based chromatin interactions enable regulated gene expression within preexisting architectural compartments. Genome Res. 2013;23:2066-77 pubmed publisher
    ..Our findings indicate that cohesin-mediated long-range interactions facilitate discrete gene expression states within preexisting chromosomal compartments. ..
  38. Ward A, Hopkins J, McKay M, Murray S, Jordan P. Genetic Interactions Between the Meiosis-Specific Cohesin Components, STAG3, REC8, and RAD21L. G3 (Bethesda). 2016;6:1713-24 pubmed publisher
    ..maintenance of chromosomes proteins (SMC1α/SMC1β and SMC3), an α-kleisin protein (RAD21, RAD21L, or REC8), and a stromal antigen protein (STAG1, 2, or 3)...
  39. Gómez H L, Felipe Medina N, Sanchez Martin M, Davies O, Ramos I, García Tuñón I, et al. C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility. Nat Commun. 2016;7:13298 pubmed publisher
    ..In accordance with its role as a modifier of the human recombination rate, SIX6OS1 is essential for the appropriate processing of intermediate recombination nodules before crossover formation. ..
  40. Burkhardt S, Borsos M, Szydlowska A, Godwin J, Williams S, Cohen P, et al. Chromosome Cohesion Established by Rec8-Cohesin in Fetal Oocytes Is Maintained without Detectable Turnover in Oocytes Arrested for Months in Mice. Curr Biol. 2016;26:678-85 pubmed publisher
    ..b>Rec8-containing cohesin, bound to Smc3/Smc1α or Smc3/Smc1β, maintains bivalent cohesion in mammalian meiosis [2-6]...
  41. Kogo H, Kowa Sugiyama H, Yamada K, Bolor H, Tsutsumi M, Ohye T, et al. Screening of genes involved in chromosome segregation during meiosis I: toward the identification of genes responsible for infertility in humans. J Hum Genet. 2010;55:293-9 pubmed publisher
    ..These 726 candidates for human infertility genes might serve as a useful resource for next-generation sequencing combined with exon capture by microarray...
  42. Eto K, Shiotsuki M, Abe S. Nociceptin induces Rec8 phosphorylation and meiosis in postnatal murine testes. Endocrinology. 2013;154:2891-9 pubmed publisher
    Phosphorylated Rec8, a key component of cohesin, mediates the association and disassociation, "dynamics," of chromosomes occurring in synaptonemal complex formation, crossover recombination, and sister chromatid cohesion during ..
  43. Koch B, Kueng S, Ruckenbauer C, Wendt K, Peters J. The Suv39h-HP1 histone methylation pathway is dispensable for enrichment and protection of cohesin at centromeres in mammalian cells. Chromosoma. 2008;117:199-210 pubmed
    ..Our data indicate that the Suv39h-HP1 pathway is not essential for enrichment and mitotic protection of cohesin at centromeres in mammalian cells. ..
  44. Kumar R, Ghyselinck N, Ishiguro K, Watanabe Y, Kouznetsova A, Höög C, et al. MEI4 – a central player in the regulation of meiotic DNA double-strand break formation in the mouse. J Cell Sci. 2015;128:1800-11 pubmed publisher
    ..We also show that MEI1, REC8 and RAD21L are important for proper MEI4 localization...
  45. Fukuda T, Pratto F, Schimenti J, Turner J, Camerini Otero R, Hoog C. Phosphorylation of chromosome core components may serve as axis marks for the status of chromosomal events during mammalian meiosis. PLoS Genet. 2012;8:e1002485 pubmed publisher
    ..many protein components of the meiotic chromosome axis, including SYCP2, SYCP3, HORMAD1, HORMAD2, SMC3, STAG3, and REC8, become post-translationally modified by phosphorylation during the prophase I stage...
  46. Guerquin M, Duquenne C, Lahaye J, Tourpin S, Habert R, Livera G. New testicular mechanisms involved in the prevention of fetal meiotic initiation in mice. Dev Biol. 2010;346:320-30 pubmed publisher