Gene Symbol: Rassf1
Description: Ras association (RalGDS/AF-6) domain family member 1
Alias: 123F2, AA536941, AU044980, NORE2A, RDA32, REH3P21, Rassf1A, Rassf1B, Rassf1C, ras association domain-containing protein 1, 123F protein, RASSF1A tumor suppressor, Ras association (RalGDS/AF-6) domain family 1, protein 123F2, rassf1
Species: mouse
Products:     Rassf1

Top Publications

  1. Thaler S, Hähnel P, Schad A, Dammann R, Schuler M. RASSF1A mediates p21Cip1/Waf1-dependent cell cycle arrest and senescence through modulation of the Raf-MEK-ERK pathway and inhibition of Akt. Cancer Res. 2009;69:1748-57 pubmed publisher
    Promoter hypermethylation preventing expression of the RAS association domain family 1 isoform A (RASSF1A) gene product is among the most abundant epigenetic deregulations in human cancer...
  2. Gordon M, El Kalla M, Zhao Y, Fiteih Y, Law J, Volodko N, et al. The tumor suppressor gene, RASSF1A, is essential for protection against inflammation -induced injury. PLoS ONE. 2013;8:e75483 pubmed publisher
    Ras association domain family protein 1A (RASSF1A) is a tumor suppressor gene silenced in cancer...
  3. Oceandy D, Pickard A, Prehar S, Zi M, Mohamed T, Stanley P, et al. Tumor suppressor Ras-association domain family 1 isoform A is a novel regulator of cardiac hypertrophy. Circulation. 2009;120:607-16 pubmed publisher
    ..Here we investigate the role of Ras effector Ras-association domain family 1 isoform A (RASSF1A) in regulating myocardial hypertrophy...
  4. Tommasi S, Dammann R, Zhang Z, Wang Y, Liu L, Tsark W, et al. Tumor susceptibility of Rassf1a knockout mice. Cancer Res. 2005;65:92-8 pubmed
    ..The two major isoforms of RASSF1, RASSF1A and RASSF1C, are distinguished by alternative NH(2)-terminal exons and the two transcripts initiate in two separate CpG ..
  5. van der Weyden L, Papaspyropoulos A, Poulogiannis G, Rust A, Rashid M, Adams D, et al. Loss of RASSF1A synergizes with deregulated RUNX2 signaling in tumorigenesis. Cancer Res. 2012;72:3817-3827 pubmed publisher
    The tumor suppressor gene RASSF1A is inactivated through point mutation or promoter hypermethylation in many human cancers...
  6. van der Weyden L, Tachibana K, Gonzalez M, Adams D, Ng B, Petty R, et al. The RASSF1A isoform of RASSF1 promotes microtubule stability and suppresses tumorigenesis. Mol Cell Biol. 2005;25:8356-67 pubmed
    The RASSF1A isoform of RASSF1 is frequently inactivated by epigenetic alterations in human cancers, but it remains unclear if and how it acts as a tumor suppressor...
  7. Tommasi S, Besaratinia A, Wilczynski S, Pfeifer G. Loss of Rassf1a enhances p53-mediated tumor predisposition and accelerates progression to aneuploidy. Oncogene. 2011;30:690-700 pubmed publisher
    Loss of RASSF1A leads to several mitotic abnormalities, including cytokinesis failure and tetraploidization...
  8. Del Re D, Matsuda T, Zhai P, Gao S, Clark G, van der Weyden L, et al. Proapoptotic Rassf1A/Mst1 signaling in cardiac fibroblasts is protective against pressure overload in mice. J Clin Invest. 2010;120:3555-67 pubmed publisher
    ..genetically altered mice in which expression of the tumor suppressor Ras-association domain family 1 isoform A (Rassf1A) was modulated in a cell type-specific manner, we demonstrate here that Rassf1A is an endogenous activator of ..
  9. van der Weyden L, Arends M, Dovey O, Harrison H, Lefebvre G, Conte N, et al. Loss of Rassf1a cooperates with Apc(Min) to accelerate intestinal tumourigenesis. Oncogene. 2008;27:4503-8 pubmed publisher
    Promoter methylation of the RAS-association domain family 1, isoform A gene (RASSF1A) is one of the most frequent events found in human tumours...

More Information


  1. Burbee D, Forgacs E, Zochbauer Muller S, Shivakumar L, Fong K, Gao B, et al. Epigenetic inactivation of RASSF1A in lung and breast cancers and malignant phenotype suppression. J Natl Cancer Inst. 2001;93:691-9 pubmed
    ..We assessed expression of two RASSF1 gene products, RASSF1A and RASSF1C, and the methylation status of their respective promoters in 27 non-small-cell lung cancer (NSCLC) ..
  2. Del Re D, Matsuda T, Zhai P, Maejima Y, Jain M, Liu T, et al. Mst1 promotes cardiac myocyte apoptosis through phosphorylation and inhibition of Bcl-xL. Mol Cell. 2014;54:639-50 pubmed publisher
    ..We here identify a signaling cassette in cardiac myocytes consisting of K-Ras, the scaffold RASSF1A, and Mst1 that is localized to mitochondria and promotes Mst1 activation in response to oxidative stress...
  3. Papaspyropoulos A, Bradley L, Thapa A, Leung C, Toskas K, Koennig D, et al. RASSF1A uncouples Wnt from Hippo signalling and promotes YAP mediated differentiation via p73. Nat Commun. 2018;9:424 pubmed publisher
    ..Here, we show that the tumour suppressor RASSF1A is a key player driving the early specification of cell fate...
  4. Jadin L, Wu X, Ding H, Frost G, Onclinx C, Triggs Raine B, et al. Skeletal and hematological anomalies in HYAL2-deficient mice: a second type of mucopolysaccharidosis IX?. FASEB J. 2008;22:4316-26 pubmed publisher
    ..In addition, the viability of HYAL2-deficient mice raises the possibility that a similar defect, defining a new MPS disorder, exists in humans. ..
  5. Schirosi L, Mazzotta A, Opinto G, Pinto R, Graziano G, Tommasi S, et al. β-catenin interaction with NHERF1 and RASSF1A methylation in metastatic colorectal cancer patients. Oncotarget. 2016;7:67841-67850 pubmed publisher
    ..Na+/H+ exchanger regulating factor 1 (NHERF1) and interacts with the RAS-association domain family 1, isoform A (RASSF1A), but the mechanisms of this possible crosstalk are still not fully understood...
  6. Zhu W, Qin W, Hewett J, Sauter E. Quantitative evaluation of DNA hypermethylation in malignant and benign breast tissue and fluids. Int J Cancer. 2010;126:474-82 pubmed publisher
  7. Wang J, Yu C, Hu X, Wu Y. Effects of nickel-smelting fumes on the regulation of NIH/3T3 cell viability, necrosis, and expression of hMLH1 and RASSF1A. J Environ Pathol Toxicol Oncol. 2014;33:1-9 pubmed
    ..of NIH/3T3 cell viability, apoptosis, and necrosis and the expression of the tumor suppressor genes hMLH1 and RASSF1A. Cell viability was determined using a methylthiazolyl tetrazolium colorimetric assay...
  8. Stieglitz B, Bee C, Schwarz D, Yildiz O, Moshnikova A, Khokhlatchev A, et al. Novel type of Ras effector interaction established between tumour suppressor NORE1A and Ras switch II. EMBO J. 2008;27:1995-2005 pubmed publisher
  9. Zhang X, Guo C, Wu X, Li A, Liu L, Tsark W, et al. Analysis of Liver Tumor-Prone Mouse Models of the Hippo Kinase Scaffold Proteins RASSF1A and SAV1. Cancer Res. 2016;76:2824-35 pubmed publisher
    The tumor suppressor gene RASSF1A is epigenetically silenced in most human cancers...
  10. Lerman M, Minna J. The 630-kb lung cancer homozygous deletion region on human chromosome 3p21.3: identification and evaluation of the resident candidate tumor suppressor genes. The International Lung Cancer Chromosome 3p21.3 Tumor Suppressor Gene Consortium. Cancer Res. 2000;60:6116-33 pubmed
  11. Song M, Song S, Kim S, Oh H, Lim D. The tumour suppressor RASSF1A promotes MDM2 self-ubiquitination by disrupting the MDM2-DAXX-HAUSP complex. EMBO J. 2008;27:1863-74 pubmed publisher
    ..MDM2 is also self-ubiquitinated and degraded. Here, we show that the tumour suppressor RASSF1A regulates G(1)-S cell-cycle progression in a p53-dependent manner by promoting MDM2 self-ubiquitination and ..
  12. Sun J, Liu J, Pan X, Quimby D, Zanesi N, Druck T, et al. Effect of zinc supplementation on N-nitrosomethylbenzylamine-induced forestomach tumor development and progression in tumor suppressor-deficient mouse strains. Carcinogenesis. 2011;32:351-8 pubmed publisher
    ..4 versus 9.2 for control mice (P < 0.01) and Zn-supplemented Fhit-/-Rassf1a-/- (the murine gene) mice averaged 5.9 versus 9.1 for control mice (P < 0.01)...
  13. Liu L, Vo A, McKeehan W. Specificity of the methylation-suppressed A isoform of candidate tumor suppressor RASSF1 for microtubule hyperstabilization is determined by cell death inducer C19ORF5. Cancer Res. 2005;65:1830-8 pubmed
    Isoform-specific epigenetic silencing of RASSF1A (3p21.3) by promoter-specific CpG island hypermethylation occurs at high frequency in human tumors, whereas the closely related product of the same gene, RASSF1C, continues to be expressed...
  14. Dallol A, Agathanggelou A, Tommasi S, Pfeifer G, Maher E, Latif F. Involvement of the RASSF1A tumor suppressor gene in controlling cell migration. Cancer Res. 2005;65:7653-9 pubmed
    We have previously shown that RASSF1A associates with the microtubules. This association alters the microtubule dynamics and seems essential for RASSF1A tumor suppressive function...
  15. Guo C, Tommasi S, Liu L, Yee J, Dammann R, Pfeifer G. RASSF1A is part of a complex similar to the Drosophila Hippo/Salvador/Lats tumor-suppressor network. Curr Biol. 2007;17:700-5 pubmed
    The Ras Association Domain Family 1A (RASSF1A) gene is one of the most frequently silenced genes in human cancer. RASSF1A has been shown to interact with the proapoptotic kinase MST1...
  16. Tommasi S, Dammann R, Jin S, Zhang X, Avruch J, Pfeifer G. RASSF3 and NORE1: identification and cloning of two human homologues of the putative tumor suppressor gene RASSF1. Oncogene. 2002;21:2713-20 pubmed
    ..RASSF3 and NORE1B are very similar, at the N-terminus, to the splice variant C of RASSF1 (RASSF1C), which does not seem to be involved in tumorigenesis...
  17. Mohamed T, Zi M, Prehar S, Maqsood A, Abou Leisa R, Nguyen L, et al. The tumour suppressor Ras-association domain family protein 1A (RASSF1A) regulates TNF-? signalling in cardiomyocytes. Cardiovasc Res. 2014;103:47-59 pubmed publisher
    ..of this study was to investigate whether the tumour suppressor Ras-association domain family protein 1 isoform A (RASSF1A) modulates TNF-? signalling in cardiomyocytes...
  18. Dmitriev R, Korneenko T, Bessonov A, Shakhparonov M, Modyanov N, Pestov N. Characterization of hampin/MSL1 as a node in the nuclear interactome. Biochem Biophys Res Commun. 2007;355:1051-7 pubmed
    ..these proteins were used as baits in library screenings and more new interactions were found: tumor suppressor RASSF1C and spliceosome component PRP3 for KIAA0103, ring finger RNF10 for RASSF1C, and RNA polymerase II regulator NELF-..
  19. Chamberlain C, Scheel D, McGlynn K, Kim H, Miyatsuka T, Wang J, et al. Menin determines K-RAS proliferative outputs in endocrine cells. J Clin Invest. 2014;124:4093-101 pubmed publisher
    ..Inhibition of proliferation by K-RAS depended on antiproliferative RAS effector RASSF1A and blockade of the RAS-activated proproliferative RAF/MAPK pathway by tumor suppressor menin...
  20. Pefani D, Latusek R, Pires I, Grawenda A, Yee K, Hamilton G, et al. RASSF1A-LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2. Nat Cell Biol. 2014;16:962-71, 1-8 pubmed publisher
    ..show that LATS1 forms part of an ATR-mediated response to replication stress that requires the tumour suppressor RASSF1A. Importantly, perturbation of the ATR-RASSF1A-LATS1 signalling axis leads to genomic defects associated with loss ..
  21. Donninger H, Clark J, Rinaldo F, Nelson N, Barnoud T, Schmidt M, et al. The RASSF1A tumor suppressor regulates XPA-mediated DNA repair. Mol Cell Biol. 2015;35:277-87 pubmed publisher
    b>RASSF1A may be the most frequently inactivated tumor suppressor identified in human cancer so far. It is a proapoptotic Ras effector and plays an important role in the apoptotic DNA damage response (DDR)...