Rag2

Summary

Gene Symbol: Rag2
Description: recombination activating gene 2
Alias: Rag-2, V(D)J recombination-activating protein 2, recombinase activating gene 2, recombination activating protein 2
Species: mouse

Top Publications

  1. pmc Overlapping signals for protein degradation and nuclear localization define a role for intrinsic RAG-2 nuclear uptake in dividing cells
    Ashley E Ross
    Department of Molecular Biology and Genetics and Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Mol Cell Biol 23:5308-19. 2003
  2. ncbi Identification of a third evolutionarily conserved gene within the RAG locus and its RAG1-dependent and -independent regulation
    Malgorzata Cebrat
    Department of Tumor Immunology, Laboratory of Transgenesis and Lymphocyte Biology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
    Eur J Immunol 35:2230-8. 2005
  3. pmc Foxo1 directly regulates the transcription of recombination-activating genes during B cell development
    Rupesh H Amin
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
    Nat Immunol 9:613-22. 2008
  4. ncbi RAG-1-deficient mice have no mature B and T lymphocytes
    P Mombaerts
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139
    Cell 68:869-77. 1992
  5. ncbi RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement
    Y Shinkai
    Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    Cell 68:855-67. 1992
  6. ncbi RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination
    M A Oettinger
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142
    Science 248:1517-23. 1990
  7. pmc Cutting edge: basophils are transiently recruited into the draining lymph nodes during helminth infection via IL-3, but infection-induced Th2 immunity can develop without basophil lymph node recruitment or IL-3
    Sohee Kim
    Department of Immunology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Immunol 184:1143-7. 2010
  8. pmc Thymic emigration revisited
    Tom M McCaughtry
    Center for Immunology, Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455
    J Exp Med 204:2513-20. 2007
  9. ncbi Positive selection through a motif in the alphabeta T cell receptor
    B T Backstrom
    Basel Institute for Immunology, CH 4005 Basel, Switzerland
    Science 281:835-8. 1998
  10. pmc No requirement for V(D)J recombination in p53-deficient thymic lymphoma
    M J Liao
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill Medical School, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 18:3495-501. 1998

Detail Information

Publications150 found, 100 shown here

  1. pmc Overlapping signals for protein degradation and nuclear localization define a role for intrinsic RAG-2 nuclear uptake in dividing cells
    Ashley E Ross
    Department of Molecular Biology and Genetics and Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Mol Cell Biol 23:5308-19. 2003
    ..These results suggest that the intrinsic RAG-2 NLS functions in the nuclear uptake of RAG-2 following its reexpression in cycling cells...
  2. ncbi Identification of a third evolutionarily conserved gene within the RAG locus and its RAG1-dependent and -independent regulation
    Malgorzata Cebrat
    Department of Tumor Immunology, Laboratory of Transgenesis and Lymphocyte Biology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
    Eur J Immunol 35:2230-8. 2005
    Recombination-activating gene (RAG)1 and RAG2 encode T and B lymphocyte-specific endonucleases indispensable for rearrangements of antigen-receptor gene segments but also capable of causing deleterious chromosome rearrangements...
  3. pmc Foxo1 directly regulates the transcription of recombination-activating genes during B cell development
    Rupesh H Amin
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
    Nat Immunol 9:613-22. 2008
    ..Foxo1 directly activated transcription of the Rag1-Rag2 locus throughout early B cell development, and a decrease in Foxo1 protein diminished the induction of Rag1 and ..
  4. ncbi RAG-1-deficient mice have no mature B and T lymphocytes
    P Mombaerts
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139
    Cell 68:869-77. 1992
    ..Although RAG-1 expression has been reported in the central nervous system of the mouse, no obvious neuroanatomical or behavioral abnormalities have been found in the RAG-1-deficient mice...
  5. ncbi RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement
    Y Shinkai
    Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    Cell 68:855-67. 1992
    ..Because the SCID phenotype was the only obvious abnormality detected in RAG-2 mutant mice, RAG-2 function and V(D)J recombinase activity, per se, are not required for development of cells other than lymphocytes...
  6. ncbi RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination
    M A Oettinger
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142
    Science 248:1517-23. 1990
    ..RAG-1 and RAG-2 might activate the expression of the V(D)J recombinase but, more likely, they directly participate in the recombination reaction...
  7. pmc Cutting edge: basophils are transiently recruited into the draining lymph nodes during helminth infection via IL-3, but infection-induced Th2 immunity can develop without basophil lymph node recruitment or IL-3
    Sohee Kim
    Department of Immunology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Immunol 184:1143-7. 2010
    ..Our results reveal a previously unknown role of IL-3 in recruiting basophils to the LN and demonstrate that basophils are not necessarily associated with the development of Th2 immunity during parasite infection...
  8. pmc Thymic emigration revisited
    Tom M McCaughtry
    Center for Immunology, Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455
    J Exp Med 204:2513-20. 2007
    ..Furthermore, emigration occurs via a strict "conveyor belt" mechanism, where the oldest thymocytes leave first...
  9. ncbi Positive selection through a motif in the alphabeta T cell receptor
    B T Backstrom
    Basel Institute for Immunology, CH 4005 Basel, Switzerland
    Science 281:835-8. 1998
    ..Thus, the alpha-CPM seems to participate in the generation of signals required for positive selection...
  10. pmc No requirement for V(D)J recombination in p53-deficient thymic lymphoma
    M J Liao
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill Medical School, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 18:3495-501. 1998
    ....
  11. ncbi CTLA-4 regulates induction of anergy in vivo
    R J Greenwald
    Immunology Research Division, Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, 02115, USA
    Immunity 14:145-55. 2001
    ..Thus, CTLA-4 has an essential role in determining the outcome of T cell encounter with a tolerogenic stimulus...
  12. pmc The RAG2 C terminus suppresses genomic instability and lymphomagenesis
    Ludovic Deriano
    Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
    Nature 471:119-23. 2011
    Misrepair of DNA double-strand breaks produced by the V(D)J recombinase (the RAG1/RAG2 proteins) at immunoglobulin (Ig) and T cell receptor (Tcr) loci has been implicated in pathogenesis of lymphoid malignancies in humans and in mice...
  13. ncbi Adaptive immunity maintains occult cancer in an equilibrium state
    Catherine M Koebel
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 450:903-7. 2007
    ..These results reveal that, in addition to destroying tumour cells and sculpting tumour immunogenicity, the immune system of a naive mouse can also restrain cancer growth for extended time periods...
  14. pmc Interferon gamma contributes to initiation of uterine vascular modification, decidual integrity, and uterine natural killer cell maturation during normal murine pregnancy
    A A Ashkar
    Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    J Exp Med 192:259-70. 2000
    ....
  15. pmc Distinct functions for the transcription factors GATA-3 and ThPOK during intrathymic differentiation of CD4(+) T cells
    Lie Wang
    Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Nat Immunol 9:1122-30. 2008
    ..We propose that GATA-3 acts as a specification factor for the CD4(+) lineage 'upstream' of the ThPOK-controlled CD4(+) commitment checkpoint...
  16. ncbi Polymorphism in Sirpa modulates engraftment of human hematopoietic stem cells
    Katsuto Takenaka
    Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada
    Nat Immunol 8:1313-23. 2007
    ..Thus, we have identified Sirpa polymorphism as a potent genetic determinant of the engraftment of human hematopoietic stem cells...
  17. ncbi Quantitative assessment of human T lymphocytes in RAG2(-/-)gammac(-/-) mice: the impact of ex vivo manipulation on in vivo functionality
    Rozemarijn S van Rijn
    Jordan Laboratory for Hemato Oncology, Department of Hematology, University Medical Center Utrecht, Utrecht, The Netherlands
    Exp Hematol 35:117-27. 2007
    ..In this study, we investigated the feasibility of using the huPBMC- RAG2(-/-)gammac(-/-) xenogeneic mouse model...
  18. pmc T-bet plays a key role in NK-mediated control of melanoma metastatic disease
    Miriam B F Werneck
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    J Immunol 180:8004-10. 2008
    ..Taken together, these results show for the first time an irreplaceable role for T-bet in the NK-mediated cross-talk between innate and adaptive immune responses to metastatic disease...
  19. pmc Human IL-3/GM-CSF knock-in mice support human alveolar macrophage development and human immune responses in the lung
    Tim Willinger
    Department of Immunobiology, The Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 108:2390-5. 2011
    ..The hIL-3/GM-CSF KI mice represent a unique mouse model that permits the study of human mucosal immune responses to lung pathogens...
  20. doi The distal V(H) gene cluster of the Igh locus contains distinct regulatory elements with Pax5 transcription factor-dependent activity in pro-B cells
    Anja Ebert
    Research Institute of Molecular Pathology, Vienna Biocenter, Dr Bohr Gasse 7, Vienna, Austria
    Immunity 34:175-87. 2011
    ..The pro-B cell-specific and Pax5-dependent activity of the PAIR elements suggests that they are involved in the regulation of distal V(H)-DJ(H) recombination at the Igh locus...
  21. ncbi The recombination activating gene-1 (RAG-1) transcript is present in the murine central nervous system
    J J Chun
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
    Cell 64:189-200. 1991
    ..The results suggest that RAG-1 functions in neurons, where its role might be to recombine elements of the neuronal genome site-specifically, or to prevent detrimental alterations of the genome in these long-lived cells...
  22. ncbi Commitment to the B-lymphoid lineage depends on the transcription factor Pax5
    S L Nutt
    Research Institute of Molecular Pathology, Vienna, Austria
    Nature 401:556-62. 1999
    ..Pax5 therefore plays an essential role in B-lineage commitment by suppressing alternative lineage choices...
  23. ncbi A conserved degradation signal regulates RAG-2 accumulation during cell division and links V(D)J recombination to the cell cycle
    Z Li
    Department of Molecular Biology and Genetics, John Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Immunity 5:575-89. 1996
    ..Using transgenic mice expressing the T490A RAG-2 mutant and a functional T cell receptor beta chain, we demonstrate that coupling of V(D)J recombination to the cell cycle is not essential for enforcement of allelic exclusion...
  24. ncbi Influence of immunoglobulin heavy- and light-chain expression on B-cell differentiation
    F Young
    Howard Hughes Medical Institute, Children s Hospital, Boston, Massachusetts 02115
    Genes Dev 8:1043-57. 1994
    ....
  25. ncbi The V(D)J recombination activating gene, RAG-1
    D G Schatz
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts 02142
    Cell 59:1035-48. 1989
    ..RAG-1 may activate V(D)J recombination indirectly, or it may encode the V(D)J recombinase itself...
  26. pmc An improved model to study tumor cell autonomous metastasis programs using MTLn3 cells and the Rag2(-/-) gammac (-/-) mouse
    Sylvia E Le Dévédec
    Division of Toxicology, Leiden Amsterdam Center for Drug Research LACDR, Leiden University, RA, Leiden, The Netherlands
    Clin Exp Metastasis 26:673-84. 2009
    ..Using the highly metastatic EGFR-overexpressing MTLn3 cell-line, we report that only Rag2(-/-)gammac(-/-) mice, which lack NK cells, allow efficient lung metastasis from primary tumors in the mammary gland...
  27. doi CX3CR1 defines functionally distinct intestinal mononuclear phagocyte subsets which maintain their respective functions during homeostatic and inflammatory conditions
    Benjamin Weber
    Division of Experimental Pathology, Institute of Pathology, University of Bern, Bern, Switzerland
    Eur J Immunol 41:773-9. 2011
    ..CX3CR1(+) iMNP can further be subdivided based on differential expression of CX3CR1. Using CX3CR1(GFP/+) ×RAG2(-/-) mice, we demonstrate that CX3CR1(hi) and CX3CR1(lo) iMNP clearly differ with respect to their morphological ..
  28. pmc B lineage-specific regulation of V(D)J recombinase activity is established in common lymphoid progenitors
    Lisa Borghesi
    Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester 01655, USA
    J Exp Med 199:491-502. 2004
    ..Evidence of this recombinase activity is detectable in all four progeny lineages (B, T, and NK, and DC), and rag2 levels are the highest in progenitor subsets immediately downstream of the CLP...
  29. pmc Role of STAT5 in controlling cell survival and immunoglobulin gene recombination during pro-B cell development
    Stephen Malin
    Research Institute of Molecular Pathology, Vienna Biocenter, Vienna, Austria
    Nat Immunol 11:171-9. 2010
    ..Hence, STAT5 and IL-7 signaling control cell survival and the developmental ordering of immunoglobulin gene rearrangements by suppressing premature Igk recombination in pro-B cells...
  30. pmc Osteoclast-derived matrix metalloproteinase-7, but not matrix metalloproteinase-9, contributes to tumor-induced osteolysis
    Sophie Thiolloy
    Department of Cancer Biology, Vanderbilt University, Nashville, Tennessee, USA
    Cancer Res 69:6747-55. 2009
    ..Our studies indicate that the selective inhibition of MMP-7 in the tumor-bone microenvironment may be of benefit for the treatment of lytic breast-to-bone metastases...
  31. pmc Cancer immunoediting of the NK group 2D ligand H60a
    Timothy O'Sullivan
    Department of Pathology, University of California at San Diego, La Jolla, CA 92093, USA
    J Immunol 187:3538-45. 2011
    ..In one of these cell lines, the H60a-hi cells could be removed by passaging the cells through RAG2(-/-) mice, resulting in edited cell lines that were poor targets for NK cells and that displayed progressive tumor ..
  32. ncbi IL-2 receptor alpha chain expression during early B lymphocyte differentiation
    J Chen
    Howard Hughes Medical Institute, Boston, MA
    Int Immunol 6:1265-8. 1994
    ..Its potential function in early B cell differentiation is discussed in comparison with its role in thymocyte differentiation...
  33. pmc Complexity of transcriptional regulation within the Rag locus: identification of a second Nwc promoter region within the Rag2 intron
    Agnieszka Laszkiewicz
    Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland
    Immunogenetics 63:183-7. 2011
    ..Here, we analyzed the phenotype of Nwc(tmpro1) mice, in which the Rag2 intragenic region containing the previously identified promoter responsible for initiating transcription of Nwc in ..
  34. ncbi A regulatory role for recombinase activating genes, RAG-1 and RAG-2, in T cell development
    J Wayne
    Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021
    Immunity 1:95-107. 1994
    ..We propose that in addition to a previously documented role in V(D)J recombination, RAG-1 and RAG-2 expression must be properly regulated for completion of normal T cell development..
  35. pmc The gene encoding early growth response 2, a target of the transcription factor NFAT, is required for the development and maturation of natural killer T cells
    Vanja Lazarevic
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Nat Immunol 10:306-13. 2009
    ..We found that Egr2 was important in the selection, survival and maturation of NKT cells. Our findings emphasize the importance of the calcineurin-NFAT-Egr2 pathway in the development of the NKT lymphocyte lineage...
  36. pmc The in vivo pattern of binding of RAG1 and RAG2 to antigen receptor loci
    Yanhong Ji
    Department of Immunobiology, Yale University School of Medicine, 300 Cedar Street, Box 208011, New Haven, CT 06520 8011, USA
    Cell 141:419-31. 2010
    The critical initial step in V(D)J recombination, binding of RAG1 and RAG2 to recombination signal sequences flanking antigen receptor V, D, and J gene segments, has not previously been characterized in vivo...
  37. doi The IL-7 signaling pathway regulates lymph node development independent of peripheral lymphocytes
    Stephane Chappaz
    Department of Biomedicine, University of Basel, Basel, Switzerland
    J Immunol 184:3562-9. 2010
    ..of thymic stromal lymphopoietin (TSLP) increased LTi cell numbers and restored LN development in IL-7(-/-) and RAG2(-/-) gamma(c)(-/-) mice...
  38. pmc RAG2's non-core domain contributes to the ordered regulation of V(D)J recombination
    John D Curry
    Division of Immunology, Department of Molecular and Cell Biology, University of California at Berkeley, 439 Life Sciences Addition, Berkeley, CA 94720 3200, USA
    Nucleic Acids Res 36:5750-62. 2008
    ..In mice lacking the non-core domain of RAG2 the ordered rearrangement is disturbed and direct V to D rearrangements are 10- to 1000-times increased in tri-..
  39. ncbi IL-7 Is essential for the development and the persistence of chronic colitis
    Teruji Totsuka
    Department of Gastroenterology and Hepatology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan
    J Immunol 178:4737-48. 2007
    ....
  40. doi Mechanism of lymphocyte-specific inactivation of RAG-2 intragenic promoter of NWC: implications for epigenetic control of RAG locus
    Malgorzata Cebrat
    Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Weigla 12, 53 114 Wroclaw, Poland
    Mol Immunol 45:2297-306. 2008
    ..The implications of our findings for understanding mechanisms regulating transcription within RAG/NWC locus in different cells are discussed and the model of epigenetic control of this locus is proposed...
  41. doi Cutting Edge: a thymocyte-thymic epithelial cell cross-talk dynamically regulates intrathymic IL-7 expression in vivo
    Nuno L Alves
    Cytokines and Lymphoid Development Unit, Institut Pasteur, Paris, France
    J Immunol 184:5949-53. 2010
    ..Although Il7(+) TECs normally diminish with age, we found that Il7(+) TECs are markedly retained in alymphoid Rag2(-/-)Il2rg(-/-) IL-7 reporter mice that manifest a profound thymopoietic arrest...
  42. pmc Conventional T-bet(+)Foxp3(-) Th1 cells are the major source of host-protective regulatory IL-10 during intracellular protozoan infection
    Dragana Jankovic
    Immunobiology Section and 2Immunopathogenesis Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 204:273-83. 2007
    ..These findings indicate that IL-10 production by CD4(+) T lymphocytes need not involve a distinct regulatory Th cell subset but can be generated in Th1 cells as part of the effector response to intracellular pathogens...
  43. ncbi Roles for common cytokine receptor gamma-chain-dependent cytokines in the generation, differentiation, and maturation of NK cell precursors and peripheral NK cells in vivo
    Christian A J Vosshenrich
    Unité des Cytokines et Développement Lymphoide, Institut Nationale de la Santé et Recherche Médicale Equipe 101, Institut Pasteur, Paris, France
    J Immunol 174:1213-21. 2005
    ..Surprisingly, the few residual NK cells generated in absence of IL-15 appear relatively mature, expressing a variety of Ly49 receptors and demonstrating lytic and cytokine production capacity...
  44. ncbi Elimination of colon cancer in germ-free transforming growth factor beta 1-deficient mice
    Sandra J Engle
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0524, USA
    Cancer Res 62:6362-6. 2002
    ..This animal model should provide insight into the protective role of TGF-beta1 in early stages of ulcerative colitis-associated human colon cancer...
  45. pmc RAG-1 and ATM coordinate monoallelic recombination and nuclear positioning of immunoglobulin loci
    Susannah L Hewitt
    Department of Pathology, New York University School of Medicine, New York, New York, USA
    Nat Immunol 10:655-64. 2009
    ..ATM activated by the cleaved allele acts in trans on the uncleaved allele to prevent biallelic recombination and chromosome breaks or translocations...
  46. ncbi Impaired B cell development and proliferation in absence of phosphoinositide 3-kinase p85alpha
    D A Fruman
    Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Science 283:393-7. 1999
    ..Lymphocyte development and function was studied with the use of the RAG2-deficient blastocyst complementation system...
  47. pmc Chimeric IgH-TCRalpha/delta translocations in T lymphocytes mediated by RAG
    Elsa Callen
    Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Cycle 8:2408-12. 2009
    ..We suggest a new model for how oncogenic translocations can arise from two non-concerted physiological DSBs...
  48. pmc Opposing regulation of T cell function by Egr-1/NAB2 and Egr-2/Egr-3
    Sam Collins
    The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Eur J Immunol 38:528-36. 2008
    ..Overall, our data suggest that just as the Egr/NAB network of genes control cell fate in other systems, TCR-induced Egr-1, 2, 3 and NAB2 control the fate of antigen recognition in T cells...
  49. ncbi Characterization of chromatin structure and enhancer elements for murine recombination activating gene-2
    Xing Cheng Wei
    Department of Immunology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Toyama, Japan
    J Immunol 169:873-81. 2002
    ..These results show that the tissue- and stage-specific expression of murine RAG-2 is regulated by alteration of the chromatin structure as well as cis-regulatory enhancer elements...
  50. pmc Interleukin 7 receptor control of T cell receptor gamma gene rearrangement: role of receptor-associated chains and locus accessibility
    S K Durum
    Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, Maryland 21702, USA
    J Exp Med 188:2233-41. 1998
    ..This data supports the model that IL-7R promotes TCR-gamma gene rearrangement by regulating accessibility of the locus via demethylation and histone acetylation of the locus...
  51. ncbi CD8+ T cells maintain tolerance to myelin basic protein by 'epitope theft'
    Antoine Perchellet
    Department of Immunology, University of Washington, Box 357650, Seattle, Washington 98195, USA
    Nat Immunol 5:606-14. 2004
    ..This form of immune regulation could limit responses of self-reactive T cells that escape other tolerance mechanisms...
  52. pmc Two distinct steps during thymocyte maturation from CD4-CD8- to CD4+CD8+ distinguished in the early growth response (Egr)-1 transgenic mice with a recombinase-activating gene-deficient background
    T Miyazaki
    Basel Institute for Immunology, Postfach CH 4005, Basel, Switzerland
    J Exp Med 186:877-85. 1997
    ..The first step, from the CD25+CD44- DN to the ISP stage, can be entirely promoted by overexpression of Egr-1...
  53. pmc B-Raf-mediated signaling pathway regulates T cell development
    Hirotake Tsukamoto
    Department of Immunogenetics, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan
    Eur J Immunol 38:518-27. 2008
    ..In conclusion, we present first evidence for the important role of B-Raf-mediated signaling in T cell development...
  54. pmc A 220-nucleotide deletion of the intronic enhancer reveals an epigenetic hierarchy in immunoglobulin heavy chain locus activation
    Tirtha Chakraborty
    Laboratory of Cellular and Molecular Biology, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
    J Exp Med 206:1019-27. 2009
    ..These observations define Emu-independent and Emu-dependent phases of locus activation that reveal an unappreciated epigenetic hierarchy in tissue-specific gene expression...
  55. ncbi Impact of negative selection on the T cell repertoire reactive to a self-peptide: a large fraction of T cell clones escapes clonal deletion
    C Bouneaud
    Unité de Biologie Moléculaire du Gène, INSERM U277, Institut Pasteur, Paris, France
    Immunity 13:829-40. 2000
    ..These results suggest that numerous low-affinity self-specific T cells persist in the periphery and show that a deleting ligand can specifically narrow the structural diversity of the TCR repertoire...
  56. ncbi A non-B-DNA structure at the Bcl-2 major breakpoint region is cleaved by the RAG complex
    Sathees C Raghavan
    Norris Comprehensive Cancer Center, Room 5428, University of Southern California Keck School of Medicine, 1441 Eastlake Ave, MC9176, Los Angeles, California 90033, USA
    Nature 428:88-93. 2004
    ..Hence, a stable non-B-DNA structure in the human genome appears to be the basis for the fragility of the Bcl-2 Mbr, and the RAG complex is able to cleave this structure...
  57. pmc Role of the individual interferon systems and specific immunity in mice in controlling systemic dissemination of attenuated pseudorabies virus infection
    P Grob
    Institute of Virology, University of Zurich, CH 8057 Zurich, Switzerland
    J Virol 73:4748-54. 1999
    ..We present evidence that mice with an intact gamma IFN system but without mature B and T cells were able to prevent systemic dissemination of gE-TK-PRV...
  58. ncbi A direct interaction between the RAG2 C terminus and the core histones is required for efficient V(D)J recombination
    Kelly L West
    Immunobiology Center, Mount Sinai School of Medicine, New York, New York 10029, USA
    Immunity 23:203-12. 2005
    ..Here, we report that RAG2 binds directly to the core histone proteins...
  59. pmc The loss of PTEN allows TCR alphabeta lineage thymocytes to bypass IL-7 and Pre-TCR-mediated signaling
    Thijs J Hagenbeek
    Department of Immunology, Netherlands Cancer Institute, Amsterdam, The Netherlands
    J Exp Med 200:883-94. 2004
    ..Analysis of mice deficient for Pten and CD3gamma; Pten and gammac; or Pten, gammac, and Rag2 revealed that deletion of PTEN can substitute for both IL-7 and pre-TCR signals...
  60. pmc Elucidation of IgH intronic enhancer functions via germ-line deletion
    Thomas Perlot
    The CBR Institute for Biomedical Research, Inc, and Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:14362-7. 2005
    ..The availability of mice with the iEmu mutation in their germ line will facilitate future studies to elucidate the roles of iEmu in V(H)(D)J(H) recombination in the context of IgH chromatin structure and germ-line transcription...
  61. ncbi Regulation of IgH gene assembly: role of the intronic enhancer and 5'DQ52 region in targeting DHJH recombination
    Roshi Afshar
    Department of Microbiology Immunology, Vanderbilt University Medical School, Nashville, TN 37232, USA
    J Immunol 176:2439-47. 2006
    ....
  62. ncbi Coordinate regulation of RAG1 and RAG2 by cell type-specific DNA elements 5' of RAG2
    W Yu
    Laboratory of Molecular Immunology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 285:1080-4. 1999
    RAG1 and RAG2 are essential for V(D)J recombination and lymphocyte development. These genes are thought to encode a transposase derived from a mobile genetic element that was inserted into the vertebrate genome 450 million years ago...
  63. ncbi Toll-like receptor 7-dependent loss of B cell tolerance in pathogenic autoantibody knockin mice
    Robert Berland
    Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Immunity 25:429-40. 2006
    ..These results demonstrate that the particular threat of nucleic acid-containing autoantigens lies in their ability to bind both antigen receptor and TLR7...
  64. pmc Roles for NBS1 in alternative nonhomologous end-joining of V(D)J recombination intermediates
    Ludovic Deriano
    Department of Pathology, The Helen L and Martin S Kimmel Center for Biology and Medicine at the Skirball Institute for Biomolecular Medicine and, New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 34:13-25. 2009
    ..These data demonstrate that the MRE11 complex functions at two distinct levels, regulating repair pathway choice (likely through enhancing the stability of DNA end complexes) and participating in alternative NHEJ of coding ends...
  65. pmc A plant homeodomain in RAG-2 that binds Hypermethylated lysine 4 of histone H3 is necessary for efficient antigen-receptor-gene rearrangement
    Yun Liu
    Department of Molecular Biology and Genetics, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Immunity 27:561-71. 2007
    ..Together, these findings are consistent with the interpretation that recognition of hypermethylated histone H3 K4 promotes efficient V(D)J recombination in vivo...
  66. pmc Development of regulatory T cells requires IL-7Ralpha stimulation by IL-7 or TSLP
    Renata Mazzucchelli
    Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute NCI, National Institutes of Health NIH, Frederick, MD, USA
    Blood 112:3283-92. 2008
    ....
  67. ncbi Rag mutations reveal robust alternative end joining
    Barbara Corneo
    The Kimmel Center for Biology and Medicine of the Skirball Institute and Department of Pathology, New York University School of Medicine, New York, New York 10016, USA
    Nature 449:483-6. 2007
    ..mechanism for generating diversity in antigen receptors, relies on NHEJ for repairing DSBs introduced by the Rag1-Rag2 protein complex. Animals lacking any of the seven known NHEJ factors are therefore immunodeficient...
  68. pmc Chromosomal reinsertion of broken RSS ends during T cell development
    John D Curry
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    J Exp Med 204:2293-303. 2007
    ..wild-type, p53, p53 x scid, H2Ax, and ATM mutant thymocytes all showed similar levels of RSS end insertions, core-RAG2 mutant thymocytes showed a sevenfold greater frequency of such events...
  69. pmc The RhoA transcriptional program in pre-T cells
    M Mullin
    Samuel Lunenfeld Research Institute, 600 University Avenue, Toronto, Ontario, Canada
    FEBS Lett 581:4309-17. 2007
    ..causes a developmental block that pheno-copies the consequence of losing pre-TCR expression in Recombinase gene 2 (Rag2) null mice...
  70. ncbi Unequal contribution of Akt isoforms in the double-negative to double-positive thymocyte transition
    Changchuin Mao
    Molecular Oncology Research Institute, Tufts New England Medical Center, 750 Washington Street, Boston, MA 02111, USA
    J Immunol 178:5443-53. 2007
    ..Akt1 and Akt2 also contribute to the differentiation process by promoting the survival of the DP thymocytes...
  71. pmc Pre-B cell receptor-mediated cell cycle arrest in Philadelphia chromosome-positive acute lymphoblastic leukemia requires IKAROS function
    Daniel Trageser
    Leukemia and Lymphoma Program, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90027, USA
    J Exp Med 206:1739-53. 2009
    ....
  72. ncbi ATM prevents the persistence and propagation of chromosome breaks in lymphocytes
    Elsa Callen
    Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 1360, USA
    Cell 130:63-75. 2007
    ..Silencing this checkpoint permits DNA ends produced by V(D)J recombination in a lymphoid precursor to serve as substrates for translocations with chromosomes subsequently damaged by other means in mature cells...
  73. ncbi Continued RAG expression in late stages of B cell development and no apparent re-induction after immunization
    W Yu
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA
    Nature 400:682-7. 1999
    ..mice carrying a bacterial artificial chromosome that encodes a green fluorescent protein reporter instead of RAG2. We find that the reporter is expressed in all immature B cells in the bone marrow and spleen...
  74. ncbi Localization, interaction, and RNA binding properties of the V(D)J recombination-activating proteins RAG1 and RAG2
    E Spanopoulou
    Rockefeller University, New York, New York 10021, USA
    Immunity 3:715-26. 1995
    The RAG1 and RAG2 gene products are indispensable for activating somatic rearrangement of antigen receptor gene segments. The two proteins form a stable complex in primary thymocytes as well as when expressed in adherent cells...
  75. pmc Deletion of the RAG2 C terminus leads to impaired lymphoid development in mice
    Yoshiko Akamatsu
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 100:1209-14. 2003
    The recombination-activating gene (RAG)1 and RAG2 proteins comprise the lymphocyte-specific components of the V(D)J recombinase and are required for the assembly of antigen-receptor variable-region genes...
  76. ncbi PU.1 regulates expression of the interleukin-7 receptor in lymphoid progenitors
    Rodney P DeKoter
    Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, 5841 S Maryland Ave, MC1028, Chicago, IL 60637, USA
    Immunity 16:297-309. 2002
    ..1 in early B cell development, it is not required. These results demonstrate that PU.1 functions to regulate early B cell development in part by controlling the expression of the IL-7Ralpha gene...
  77. ncbi Enhanced human cell engraftment in mice deficient in RAG2 and the common cytokine receptor gamma chain
    J P Goldman
    Molecular Immunology Unit, Institute of Child Health, London, UK
    Br J Haematol 103:335-42. 1998
    ..A genetic cross with a recombinase activating gene 2 (RAG2)-deficient strain produced mice doubly homozygous for the gamma c and RAG2 null alleles (gamma c-..
  78. ncbi Modulation of the IL-7 dose-response threshold during pro-B cell differentiation is dependent on pre-B cell receptor expression
    A J Marshall
    Ontario Cancer Institute and Department of Immunology, University of Toronto, Canada
    J Immunol 161:6038-45. 1998
    ..Our results suggest that positive selection of pre-BCR+ pro-B cells may be achieved through the fine tuning of IL-7 responses...
  79. ncbi Increased accumulation of hybrid V(D)J joins in cells expressing truncated versus full-length RAGs
    J A Sekiguchi
    Howard Hughes Medical Institute, Harvard University Medical School, Boston, MA 02115, USA
    Mol Cell 8:1383-90. 2001
    RAG1 and RAG2 (RAGs) initiate V(D)J recombination by introducing breaks between two coding segments and flanking recombination signals (RSs). Nonhomologous end-joining (NHEJ) proteins then join the coding segments and join the RSs...
  80. ncbi Cell-fate decisions in early T cell development: regulation by cytokine receptors and the pre-TCR
    M C Haks
    Division of Immunology, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Huis, Plesmanlaan 121, Amsterdam, CX, 1066, The Netherlands
    Semin Immunol 11:23-37. 1999
    ....
  81. pmc Induction of pre-B cell proliferation after de novo synthesis of the pre-B cell receptor
    J Hess
    Institut für Medizinische Strahlenkunde und Zellforschung MSZ, University of Wurzburg, Versbacher Strasse 5, D 97078 Wurzburg, Germany
    Proc Natl Acad Sci U S A 98:1745-50. 2001
    ..We conclude that pre-BCR signaling induces clonal expansion of early pre-B cells...
  82. ncbi Dendritic cells directly trigger NK cell functions: cross-talk relevant in innate anti-tumor immune responses in vivo
    N C Fernandez
    Departement de Biologie Clinique, Institut Gustave Roussy, Villejuif, France
    Nat Med 5:405-11. 1999
    ..Thus, DC are involved in the interaction between innate and adaptive immune responses...
  83. pmc Recombinase-activating gene (RAG) 2-mediated V(D)J recombination is not essential for tumorigenesis in Atm-deficient mice
    L K Petiniot
    Experimental Immunology Branch, Genetics Department, Biostatistics and Data Management Section, Office of the Director of the Division of Clinical Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Proc Natl Acad Sci U S A 97:6664-9. 2000
    ..Since V(D)J recombination is a recombinase-activating gene (RAG)-dependent process, we generated Rag2(-/-)Atm(-/-) mice to assess the requirement for RAG-dependent recombination in thymic lymphomagenesis...
  84. pmc B cell development in the spleen takes place in discrete steps and is determined by the quality of B cell receptor-derived signals
    F Loder
    Department of Molecular Immunology, University of Freiburg, Germany
    J Exp Med 190:75-89. 1999
    ....
  85. ncbi RAG2:GFP knockin mice reveal novel aspects of RAG2 expression in primary and peripheral lymphoid tissues
    R J Monroe
    Howard Hughes Medical Institute, The Children s Hospital, The Center for Blood Research and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Immunity 11:201-12. 1999
    We generated mice in which a functional RAG2:GFP fusion gene is knocked in to the endogenous RAG2 locus...
  86. ncbi The earliest T lineage-committed cells depend on IL-7 for Bcl-2 expression and normal cell cycle progression
    U von Freeden-Jeffry
    Department of Immunobiology, DNAX Research Institute, Palo Alto, California 94304, USA
    Immunity 7:147-54. 1997
    ..These data specify a T cell lineage developmental transition point, prior to T cell antigen receptor rearrangement, where IL-7 signal transduction is linked to an anti-apoptosis mechanism and the cell cycle...
  87. pmc Cell cycle regulation of V(D)J recombination-activating protein RAG-2
    W C Lin
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205
    Proc Natl Acad Sci U S A 91:2733-7. 1994
    ..The variation in RAG-2 protein is likely to be established, at least in part, by a posttranscriptional mechanism. These observations suggest that V(D)J rearrangement occurs entirely or preferentially within G0/G1...
  88. ncbi Restoration of T cell development in RAG-2-deficient mice by functional TCR transgenes
    Y Shinkai
    Howard Hughes Medical Institute, Children s Hospital, Boston, MA 02115
    Science 259:822-5. 1993
    ..RAG-2-/- mice that expressed alpha and beta TCR transgenes developed both DP and single positive thymocytes. Thus, the TCR beta subunit, possibly in association with a novel CD3 complex, participates in the DN to the DP transition...
  89. ncbi Essential role of LAT in T cell development
    W Zhang
    Section on Lymphocyte Signaling, Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Immunity 10:323-32. 1999
    ..Intrathymic development was blocked within the CD4- CD8- stage. No gross abnormality of NK or platelet function was observed. LAT is thus critical to both T cell activation and development...
  90. pmc T cell receptor-independent basal signaling via Erk and Abl kinases suppresses RAG gene expression
    Jeroen P Roose
    Department of Medicine, University of California, San Francisco, USA
    PLoS Biol 1:E53. 2003
    ..Our data suggest that physiologic gene expression programs depend upon tonic activity of signaling pathways independent of receptor ligation...
  91. ncbi A conserved transcriptional enhancer regulates RAG gene expression in developing B cells
    Lih Yun Hsu
    Division of Immunology, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Immunity 19:105-17. 2003
    Although expression of the RAG1 and RAG2 genes is essential for lymphocyte development, the mechanisms responsible for the lymphoid- and developmental stage-specific regulation of these genes are poorly understood...
  92. pmc DNA sequence and structure requirements for cleavage of V(D)J recombination signal sequences
    C A Cuomo
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 16:5683-90. 1996
    Purified RAG1 and RAG2 proteins can cleave DNA at V(D)J recombination signals...
  93. ncbi Down-regulation of RAG1 and RAG2 gene expression in preB cells after functional immunoglobulin heavy chain rearrangement
    U Grawunder
    Basel Institute for Immunology, Switzerland
    Immunity 3:601-8. 1995
    ..The gene products of two recombination activating genes, RAG1 and RAG2 are crucial for this rearrangement process...
  94. ncbi Intradermal administration of thymic stromal lymphopoietin induces a T cell- and eosinophil-dependent systemic Th2 inflammatory response
    Heidi K Jessup
    Inflammation Research, Amgen, Seattle, WA 98119, USA
    J Immunol 181:4311-9. 2008
    ..TSLP-induced responses were significantly, but not completely reduced in IL-4- and IL-13-deficient mice. These results shed light on the pathways and cell types involved in TSLP-induced inflammation...
  95. pmc T cell receptor contact to restricting MHC molecules is a prerequisite for peripheral interclonal T cell competition
    Fabien Agenes
    Institut National de la Santé et de la Recherche Médicale U548, Grenoble, Cedex 9, France
    J Exp Med 205:2735-43. 2008
    ..This mechanism allows a local competitiveness that extends beyond a T cell's specificity...
  96. ncbi Impaired expansion of mouse B cell progenitors lacking Btk
    J D Kerner
    Department of Immunology, University of Washington, Seattle 98195, USA
    Immunity 3:301-12. 1995
    ..a targeted disruption of the btk gene and examined their potential to produce lymphocytes in both C57BL/6 and RAG2-/- host chimeric animals...
  97. pmc Bystander activation of cytotoxic T cells: studies on the mechanism and evaluation of in vivo significance in a transgenic mouse model
    S Ehl
    Institute of Experimental Immunology, Department of Pathology, University of Zurich, Switzerland
    J Exp Med 185:1241-51. 1997
    ..We conclude that although bystander activation via cytokines may generate cytolytically active CTL from naive precursors, quantitative considerations suggest that this is usually not of major biological consequence...
  98. pmc Deletion of Shp2 tyrosine phosphatase in muscle leads to dilated cardiomyopathy, insulin resistance, and premature death
    Frederic Princen
    Burnham Institute for Medical Research, 10901 N Torrey Pines Rd, La Jolla, CA 92037, USA
    Mol Cell Biol 29:378-88. 2009
    ....
  99. pmc CTCF regulates cell cycle progression of alphabeta T cells in the thymus
    Helen Heath
    Department of Cell Biology and Genetics, Erasmus MC, Rotterdam, The Netherlands
    EMBO J 27:2839-50. 2008
    ..We propose that CTCF positively regulates cell growth in rapidly dividing thymocytes so that appropriate number of cells are generated before positive and negative selection in the thymus...
  100. pmc T cells, but not B cells, are required for bowel inflammation in interleukin 2-deficient mice
    A Ma
    Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Exp Med 182:1567-72. 1995
    ..IL-2-/-, JH-/- mice do not, however, succumb to anemia. Thus, spontaneous intestinal inflammation in IL-2-/- mice requires mature T cells, not B cells, while anemia is dependent on B cells...
  101. pmc Development of CD4+CD8+ thymocytes in RAG-deficient mice through a T cell receptor beta chain-independent pathway
    C J Guidos
    Division of Immunology and Cancer, Hospital for Sick Children Research Institute, Toronto, Canada
    J Exp Med 181:1187-95. 1995
    ..These results suggest an in vivo pathway for DP thymocyte development which is TCR beta chain independent...

Research Grants62

  1. An Animal Model of Hemophagocytic Lymphohistiocytosis
    Michael Jordan; Fiscal Year: 2009
    ..This project will lead to better understanding of how cytotoxic function regulates the immune response and lead to improved therapies for patients with HLH and perhaps many other immunopathologic disorders. ..
  2. Role of IL10 and TGFb1 in colon cancer
    Susan E Erdman; Fiscal Year: 2010
    ..We propose to build upon our recent scientific breakthroughs and test strategies in mice that will stimulate potent and highly beneficial Treg to prevent cancer and impart healthful longevity in humans. ..
  3. Role of IL10 and TGFb1 in colon cancer
    SUSAN ERDMAN; Fiscal Year: 2007
    ..Information gained from these studies may elucidate pivotal mechanisms and uncover novel strategies for prevention and treatment of human colon cancer. ..
  4. The role of T-cells and Th 1 immunity in cholesterol gallstones
    KIRK MAURER; Fiscal Year: 2007
    ..To accomplish this aim T cell subsets (effector T-cells and regulatory T cells) will be transferred to Rag 2-/- mice...
  5. An Animal Model of Hemophagocytic Lymphohistiocytosis
    Michael Jordan; Fiscal Year: 2007
    ..This project will lead to better understanding of how cytotoxic function regulates the immune response and lead to improved therapies for patients with HLH and perhaps many other immunopathologic disorders. ..
  6. CIS-REGULATORY ANALYSIS OF PU.1 BY IN VITRO ES CELL DIFFERENTIATION
    ELLEN ROTHENBERG; Fiscal Year: 2007
    ..This should permit faster and cheaper experiments to reveal how powerful regulatory genes are themselves controlled. ..
  7. Clonal analysis of joint-infiltrating natural Killer cells in chronic arthritis
    Lisa Borghesi; Fiscal Year: 2007
    ..Characterizing the developmental dynamics and functional activities of recombination* inflammatory NK cells could provide insights into the development of alternative therapies for RA. ..
  8. Mouse Models of Tumor Progression and Therapy Response
    CHRISTOPHER KEMP; Fiscal Year: 2007
    ..These experiments will reveal the molecular and cellular basis for the poor clinical outcome associated with p27 deficient tumors. This knowledge can then be applied to improve treatment design and regimen in the clinical setting. ..
  9. Experimental transfusion-related acute lung injury
    Mark Looney; Fiscal Year: 2007
    ..Looney, and at the end of the grant period, Dr. Looney will be well equipped to embark on a successful career as an independent investigator in the field of acute lung injury. (End of Abstract) ..
  10. GATA-3 in precursor commitment to the T lineage
    ELLEN ROTHENBERG; Fiscal Year: 2007
    ..Definition of mechanistic requirements for GATA-3 perturbation of T-cell development 3. Attempted rescue of GATA-3 overexpression effects by cotransduction with targets of specific repression ..
  11. ANALYSIS OF THE MURINE ANTIBODY REPERTOIRE
    Garnett Kelsoe; Fiscal Year: 2007
    ..These immature cells proliferate and express the RAG2 recombinase protein but not BCL-6, a trans- criptional repressor thought to be absolutely required for the GC ..
  12. An Animal Model of Hemophagocytic Lymphohistiocytosis
    Michael Jordan; Fiscal Year: 2010
    ..This project will lead to better understanding of how cytotoxic function regulates the immune response and lead to improved therapies for patients with HLH and perhaps many other immunopathologic disorders. ..
  13. B-cell Tolerance and Humoral Immunity to HIV-1
    GARNETT H KELSOE; Fiscal Year: 2010
    ..abstract_text> ..
  14. ANALYSIS OF THE MURINE ANTIBODY REPERTOIRE
    GARNETT H KELSOE; Fiscal Year: 2010
    ..Qom cap mop axiom 0-c 0opccc woo'- t00 0co -td ..
  15. An Animal Model of Hemophagocytic Lymphohistiocytosis
    Michael Jordan; Fiscal Year: 2010
    ..This project will lead to better understanding of how cytotoxic function regulates the immune response and lead to improved therapies for patients with HLH and perhaps many other immunopathologic disorders. ..
  16. Mechanisms of Pulmonary Mast Cell Recruitment and Maturation
    Michael Gurish; Fiscal Year: 2010
    ....
  17. LINEAGE COMMITMENT MECHANISMS IN LYMPHOID PRECURSORS
    Ellen V Rothenberg; Fiscal Year: 2010
    ..The mechanisms studied here imply that mixed-lineage thymic lymphomas may also result from primary defects of a natural T/myeloid lineage choice. ..
  18. Basal Ig Signaling and Receptor Editing
    Michael Farrar; Fiscal Year: 2009
    ..abstract_text> ..
  19. Factors that modulate thymic output
    Thomas Serwold; Fiscal Year: 2006
    ..In addition, it will be directly relevant to therapies that would involve skewing of T cell development to favor T cells with desired specificities. ..
  20. ANALYSIS OF THE MURINE ANTIBODY REPERTOIRE
    Garnett Kelsoe; Fiscal Year: 2003
    ..These immature cells proliferate and express the RAG2 recombinase protein but not BCL-6, a transcriptional repressor thought to be absolutely required for the GC ..
  21. GATA-3 in precursor commitment to the T lineage
    ELLEN ROTHENBERG; Fiscal Year: 2003
    ..Definition of mechanistic requirements for GATA-3 perturbation of T-cell development 3. Attempted rescue of GATA-3 overexpression effects by cotransduction with targets of specific repression. ..
  22. Application for a Fluorescence-Activated Cell Sorter
    ELLEN ROTHENBERG; Fiscal Year: 2003
    ..This application is to replace the old sorter with a new sorter that will improve service to existing clients and permit the Facility to carry out new flow cytometric applications that have not been available to the user group before. ..
  23. GATA-3 in precursor commitment to the T lineage
    ELLEN ROTHENBERG; Fiscal Year: 2006
    ..Definition of mechanistic requirements for GATA-3 perturbation of T-cell development 3. Attempted rescue of GATA-3 overexpression effects by cotransduction with targets of specific repression. ..
  24. B Cell Expansion After Dendritic Cell Immunization
    Michael Jordan; Fiscal Year: 2004
    ..My near term career path will involve ongoing basic research and study in order to improve my ultimate scientific productivity. ..
  25. LINEAGE COMMITMENT MECHANISMS IN LYMPHOID PRECURSORS
    ELLEN ROTHENBERG; Fiscal Year: 2005
    ..1 itself. ..