Rad51

Summary

Gene Symbol: Rad51
Description: RAD51 recombinase
Alias: AV304093, Rad51a, Reca, DNA repair protein RAD51 homolog 1, RAD51 homolog A
Species: mouse
Products:     Rad51

Top Publications

  1. Daniel K, Lange J, Hached K, Fu J, Anastassiadis K, Roig I, et al. Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1. Nat Cell Biol. 2011;13:599-610 pubmed publisher
  2. Petukhova G, Pezza R, Vanevski F, Ploquin M, Masson J, Camerini Otero R. The Hop2 and Mnd1 proteins act in concert with Rad51 and Dmc1 in meiotic recombination. Nat Struct Mol Biol. 2005;12:449-53 pubmed
    ..b>Rad51 and Dmc1 recombinases are the major players in these processes...
  3. Barlow A, Benson F, West S, Hulten M. Distribution of the Rad51 recombinase in human and mouse spermatocytes. EMBO J. 1997;16:5207-15 pubmed
    In vitro, the human Rad51 protein (hRad51) promotes homologous pairing and strand exchange reactions suggestive of a key role in genetic recombination...
  4. Soper S, van der Heijden G, Hardiman T, Goodheart M, Martin S, de Boer P, et al. Mouse maelstrom, a component of nuage, is essential for spermatogenesis and transposon repression in meiosis. Dev Cell. 2008;15:285-97 pubmed publisher
    ..This study demonstrates that MAEL, a nuage component, is indispensable for the silencing of TEs and identifies the initiation of meiosis as an important step in TE control in the male germline. ..
  5. Tachibana M, Nozaki M, Takeda N, Shinkai Y. Functional dynamics of H3K9 methylation during meiotic prophase progression. EMBO J. 2007;26:3346-59 pubmed
    ..This genetic and biochemical evidence strongly suggests that a specific set of H3K9 methyltransferase(s) and demethylase(s) coordinately regulate gametogenesis. ..
  6. Wong J, Alon N, McKerlie C, Huang J, Meyn M, Buchwald M. Targeted disruption of exons 1 to 6 of the Fanconi Anemia group A gene leads to growth retardation, strain-specific microphthalmia, meiotic defects and primordial germ cell hypoplasia. Hum Mol Genet. 2003;12:2063-76 pubmed
    ..However, the localization of Rad51, Brca1, Fancd2 and Mlh1 appeared normal on Fanca(tm1Hsc) homozygous meiotic chromosomes...
  7. Houghtaling S, Timmers C, Noll M, Finegold M, Jones S, Meyn M, et al. Epithelial cancer in Fanconi anemia complementation group D2 (Fancd2) knockout mice. Genes Dev. 2003;17:2021-35 pubmed
    ..The phenotypic overlap between Fancd2-null and Brca2/Fancd1 hypomorphic mice is consistent with a common function for both proteins in the same pathway, regulating genomic stability. ..
  8. Holloway J, Mohan S, Balmus G, Sun X, Modzelewski A, Borst P, et al. Mammalian BTBD12 (SLX4) protects against genomic instability during mammalian spermatogenesis. PLoS Genet. 2011;7:e1002094 pubmed publisher
    ..However, DSB repair is delayed or impeded, resulting in persistent ?H2AX and RAD51, and the choice of repair pathway may be altered, resulting in elevated MLH1/MLH3 focus numbers at pachynema...
  9. Yang F, Eckardt S, Leu N, McLaughlin K, Wang P. Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis. J Cell Biol. 2008;180:673-9 pubmed publisher
    ..mutant spermatocytes, DNA double-strand breaks (DSBs) are formed, but localization of the recombination proteins RAD51 and DMC1 to meiotic chromosomes is severely impaired...

More Information

Publications79

  1. Cole F, Kauppi L, Lange J, Roig I, Wang R, Keeney S, et al. Homeostatic control of recombination is implemented progressively in mouse meiosis. Nat Cell Biol. 2012;14:424-30 pubmed publisher
    ..In wild-type mouse spermatocytes, focus numbers for early recombination proteins (RAD51, DMC1) were highly variable from cell to cell, whereas foci of the crossover marker MLH1 showed little variability...
  2. Shinohara A, Ogawa H, Matsuda Y, Ushio N, Ikeo K, Ogawa T. Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA. Nat Genet. 1993;4:239-43 pubmed
    b>Rad51, of Saccharomyces cerevisiae, is a homologue of recA of Escherichia coli and plays crucial roles in both mitotic and meiotic recombination and in repair of double-strand breaks of DNA...
  3. Schramm S, Fraune J, Naumann R, Hernández Hernández A, Hoog C, Cooke H, et al. A novel mouse synaptonemal complex protein is essential for loading of central element proteins, recombination, and fertility. PLoS Genet. 2011;7:e1002088 pubmed publisher
    ..We conclude that SYCE3 enables chromosome loading of the other CE-specific proteins, which in turn would promote synapsis between homologous chromosomes...
  4. Kouznetsova A, Benavente R, Pastink A, Hoog C. Meiosis in mice without a synaptonemal complex. PLoS ONE. 2011;6:e28255 pubmed publisher
  5. Stark J, Pierce A, Oh J, Pastink A, Jasin M. Genetic steps of mammalian homologous repair with distinct mutagenic consequences. Mol Cell Biol. 2004;24:9305-16 pubmed
    ..We observed an inverse relationship between HDR and SSA when RAD51 or BRCA2 was impaired, i.e., HDR was reduced but SSA was increased...
  6. Stark J, Hu P, Pierce A, Moynahan M, Ellis N, Jasin M. ATP hydrolysis by mammalian RAD51 has a key role during homology-directed DNA repair. J Biol Chem. 2002;277:20185-94 pubmed
    Disruption of the gene encoding RAD51, the protein that catalyzes strand exchange during homologous recombination, leads to the accumulation of chromosome breaks and lethality in vertebrate cells...
  7. Lim D, Hasty P. A mutation in mouse rad51 results in an early embryonic lethal that is suppressed by a mutation in p53. Mol Cell Biol. 1996;16:7133-43 pubmed
    b>RecA in Escherichia coli and its homolog, ScRad51 in Saccharomyces cerevisiae, are known to be essential for recombinational repair. The homolog of RecA and ScRad51 in mice, MmRad51, was mutated to determine its function...
  8. Tsuzuki T, Fujii Y, Sakumi K, Tominaga Y, Nakao K, Sekiguchi M, et al. Targeted disruption of the Rad51 gene leads to lethality in embryonic mice. Proc Natl Acad Sci U S A. 1996;93:6236-40 pubmed
    The mouse Rad51 gene is a mammalian homologue of the Escherichia coli recA and yeast RAD51 genes, both of which are involved in homologous recombination and DNA repair...
  9. Sengupta S, Robles A, Linke S, Sinogeeva N, Zhang R, Pedeux R, et al. Functional interaction between BLM helicase and 53BP1 in a Chk1-mediated pathway during S-phase arrest. J Cell Biol. 2004;166:801-13 pubmed
    ..Once the ATR/Chk1- and 53BP1-mediated signal from replicational stress is received, BLM functions in multiple downstream repair processes, thereby fulfilling its role as a caretaker tumor suppressor. ..
  10. Libby B, Reinholdt L, Schimenti J. Positional cloning and characterization of Mei1, a vertebrate-specific gene required for normal meiotic chromosome synapsis in mice. Proc Natl Acad Sci U S A. 2003;100:15706-11 pubmed
    ..Homozygotes of both sexes are sterile due to meiotic arrest caused by defects in chromosome synapsis. Notably, RAD51 protein does not load onto Mei1 mutant meiotic chromosomes, suggesting that there is a defect in either ..
  11. Adelman C, Lolo R, Birkbak N, Murina O, Matsuzaki K, Horejsi Z, et al. HELQ promotes RAD51 paralogue-dependent repair to avert germ cell loss and tumorigenesis. Nature. 2013;502:381-4 pubmed publisher
    ..We establish that HELQ interacts directly with the RAD51 paralogue complex BCDX2 and functions in parallel to the Fanconi anaemia pathway to promote efficient homologous ..
  12. Herrán Y, Gutiérrez Caballero C, Sanchez Martin M, Hernandez T, Viera A, Barbero J, et al. The cohesin subunit RAD21L functions in meiotic synapsis and exhibits sexual dimorphism in fertility. EMBO J. 2011;30:3091-105 pubmed publisher
    ..Thus, our results provide in vivo evidence that RAD21L is essential for male fertility and in females for the maintenance of fertility during natural aging. ..
  13. Lipkin S, Moens P, Wang V, Lenzi M, Shanmugarajah D, Gilgeous A, et al. Meiotic arrest and aneuploidy in MLH3-deficient mice. Nat Genet. 2002;31:385-90 pubmed
    ..Mlh3(-/-) spermatocytes reach metaphase before succumbing to apoptosis, but oocytes fail to complete meiosis I after fertilization. Our results show that Mlh3 has an essential and distinct role in mammalian meiosis. ..
  14. Bellani M, Boateng K, McLeod D, Camerini Otero R. The expression profile of the major mouse SPO11 isoforms indicates that SPO11beta introduces double strand breaks and suggests that SPO11alpha has an additional role in prophase in both spermatocytes and oocytes. Mol Cell Biol. 2010;30:4391-403 pubmed publisher
    ..Altogether, our data support a role for SPO11alpha in mid- to late prophase, presumably acting as a topoisomerase, that would be conserved in male and female meiocytes. ..
  15. Kneitz B, Cohen P, Avdievich E, Zhu L, Kane M, Hou H, et al. MutS homolog 4 localization to meiotic chromosomes is required for chromosome pairing during meiosis in male and female mice. Genes Dev. 2000;14:1085-97 pubmed
    ..Although meiosis is initiated in Msh4 mutant male and female mice, as indicated by the chromosomal localization of RAD51 and COR1 during leptonema/zygonema, the chromosomes fail to undergo normal pairing...
  16. Akamatsu Y, Jasin M. Role for the mammalian Swi5-Sfr1 complex in DNA strand break repair through homologous recombination. PLoS Genet. 2010;6:e1001160 pubmed publisher
    ..Mouse Swi5 and Sfr1 are nuclear proteins that form a complex in vivo and in vitro. Swi5 interacts in vitro with Rad51, the DNA strand-exchange protein which functions during HR...
  17. Wesoly J, Agarwal S, Sigurdsson S, Bussen W, Van Komen S, Qin J, et al. Differential contributions of mammalian Rad54 paralogs to recombination, DNA damage repair, and meiosis. Mol Cell Biol. 2006;26:976-89 pubmed
    Homologous recombination is a versatile DNA damage repair pathway requiring Rad51 and Rad54...
  18. Lee E, Won Y, Kim B, Park D, Bae J, Park S, et al. Low-dose irradiation promotes Rad51 expression by down-regulating miR-193b-3p in hepatocytes. Sci Rep. 2016;6:25723 pubmed publisher
    ..01 Gy. However, the alteration of histone deacetylation and miR-193b-3p and Rad51 expression in response to LDIR was restored by pretreatment with N-acetyl-cyctein...
  19. Depienne C, Bouteiller D, Méneret A, Billot S, Groppa S, Klebe S, et al. RAD51 haploinsufficiency causes congenital mirror movements in humans. Am J Hum Genet. 2012;90:301-7 pubmed publisher
    ..analysis and exome sequencing, we identified heterozygous mutations introducing premature termination codons in RAD51 in two families with CMM...
  20. Slupianek A, Dasgupta Y, Ren S, Gurdek E, Donlin M, Nieborowska Skorska M, et al. Targeting RAD51 phosphotyrosine-315 to prevent unfaithful recombination repair in BCR-ABL1 leukemia. Blood. 2011;118:1062-8 pubmed publisher
    ..Here we show that BCR-ABL1 (nonmutated and T315I mutant) promoted RAD51 recombinase-mediated unfaithful homeologous recombination repair (HomeoRR) in a dosage-dependent manner...
  21. Kitao H, Yuan Z. Regulation of ionizing radiation-induced Rad52 nuclear foci formation by c-Abl-mediated phosphorylation. J Biol Chem. 2002;277:48944-8 pubmed
    The RAD52 epistasis group of proteins, including Rad51, Rad52, and Rad54, plays an important role in the homologous recombination repair of double strand breaks...
  22. Kawabata M, Saeki K. Sequence analysis and expression of a novel mouse homolog of Escherichia coli recA gene. Biochim Biophys Acta. 1998;1398:353-8 pubmed
    Escherichia coli recA and its yeast homologs RAD51 and DMC1 play crucial roles in mitotic and/or meiotic recombination and in repair of double-strand DNA breaks. We have identified a murine novel recA-like gene (MmTRAD)...
  23. Kim T, Ko J, Hu L, Kim S, Bishop A, Vijg J, et al. RAD51 mutants cause replication defects and chromosomal instability. Mol Cell Biol. 2012;32:3663-80 pubmed publisher
    b>RAD51 is important for restarting stalled replication forks and for repairing DNA double-strand breaks (DSBs) through a pathway called homology-directed repair (HDR)...
  24. Tambini C, Spink K, Ross C, Hill M, Thacker J. The importance of XRCC2 in RAD51-related DNA damage repair. DNA Repair (Amst). 2010;9:517-25 pubmed publisher
    ..The central HR protein is RAD51, which ensures high fidelity DNA repair by facilitating strand exchange between damaged and undamaged homologous ..
  25. Kwan K, Moens P, Wang J. Infertility and aneuploidy in mice lacking a type IA DNA topoisomerase III beta. Proc Natl Acad Sci U S A. 2003;100:2526-31 pubmed
    ..pachytene, and that there is a time lag between the appearance of chromosome-bound DNA topoisomerase III beta and Rad51, a protein known to be involved in an early step of homologous recombination...
  26. Morita T, Yoshimura Y, Yamamoto A, Murata K, Mori M, Yamamoto H, et al. A mouse homolog of the Escherichia coli recA and Saccharomyces cerevisiae RAD51 genes. Proc Natl Acad Sci U S A. 1993;90:6577-80 pubmed
    ..In budding yeast, Saccharomyces cerevisiae, the RAD51 gene is essential along with other genes of the RAD52 epistasis group for mitotic and meiotic recombination and ..
  27. Boichuk S, Hu L, Makielski K, Pandolfi P, Gjoerup O. Functional connection between Rad51 and PML in homology-directed repair. PLoS ONE. 2011;6:e25814 pubmed publisher
    ..antigen (LT) induces DNA damage and, consequently, nuclear foci of the key homologous recombination repair protein Rad51 that colocalize with PML. PML depletion abrogates LT-induced Rad51 foci...
  28. Berkowitz K, Sowash A, Koenig L, Urcuyo D, Khan F, Yang F, et al. Disruption of CHTF18 causes defective meiotic recombination in male mice. PLoS Genet. 2012;8:e1002996 pubmed publisher
    ..Repair of DNA double-strand breaks is delayed and incomplete; both RAD51 and ?H2AX persist in prophase I. In addition, MLH1 foci are decreased in pachynema...
  29. Urbanska K, Pannizzo P, Lassak A, Gualco E, Surmacz E, Croul S, et al. Estrogen receptor beta-mediated nuclear interaction between IRS-1 and Rad51 inhibits homologous recombination directed DNA repair in medulloblastoma. J Cell Physiol. 2009;219:392-401 pubmed publisher
    ..Following cisplatin-induced DNA damage, nuclear IRS-1 localized at the sites of damaged DNA, and interacted with Rad51--an enzymatic component of homologous recombination directed DNA repair (HRR)...
  30. Sharan S, Morimatsu M, Albrecht U, Lim D, Regel E, Dinh C, et al. Embryonic lethality and radiation hypersensitivity mediated by Rad51 in mice lacking Brca2. Nature. 1997;386:804-10 pubmed
    ..the mouse Brca2 gene is now defined and an interaction identified of the Brca2 protein with the DNA-repair protein Rad51. Developmental arrest in Brca2-deficient embryos, their radiation sensitivity, and the association of Brca2 with ..
  31. Simandlova J, Zagelbaum J, Payne M, Chu W, Shevelev I, Hanada K, et al. FBH1 helicase disrupts RAD51 filaments in vitro and modulates homologous recombination in mammalian cells. J Biol Chem. 2013;288:34168-80 pubmed publisher
    ..A key player in HR is RAD51, the eukaryotic ortholog of bacterial RecA protein...
  32. Bugreev D, Huang F, Mazina O, Pezza R, Voloshin O, Camerini Otero R, et al. HOP2-MND1 modulates RAD51 binding to nucleotides and DNA. Nat Commun. 2014;5:4198 pubmed publisher
    ..In vitro, HOP2-MND1 stimulates the DNA strand exchange activities of RAD51 and DMC1...
  33. Holloway J, Sun X, Yokoo R, Villeneuve A, Cohen P. Mammalian CNTD1 is critical for meiotic crossover maturation and deselection of excess precrossover sites. J Cell Biol. 2014;205:633-41 pubmed publisher
  34. Yamaguchi S, Hong K, Liu R, Shen L, Inoue A, Diep D, et al. Tet1 controls meiosis by regulating meiotic gene expression. Nature. 2012;492:443-7 pubmed publisher
    ..Our study thus establishes a function for Tet1 in meiosis and meiotic gene activation in female germ cells. ..
  35. Sioftanos G, Ismail A, Föhse L, Shanley S, Worku M, Short S. BRCA1 and BRCA2 heterozygosity in embryonic stem cells reduces radiation-induced Rad51 focus formation but is not associated with radiosensitivity. Int J Radiat Biol. 2010;86:1095-105 pubmed publisher
    ..break (DSB) repair, whose functions include facilitating homologous recombination through interactions with Rad51, the human homologue of bacterial RecA...
  36. Cherry S, Adelman C, Theunissen J, Hassold T, Hunt P, Petrini J. The Mre11 complex influences DNA repair, synapsis, and crossing over in murine meiosis. Curr Biol. 2007;17:373-8 pubmed
    ..Thus, our findings implicate the Mre11 complex in meiotic DNA repair and synapsis in mammals and indicate that the complex may contribute to the establishment of normal sex-specific differences in meiosis...
  37. Badie S, Escandell J, Bouwman P, Carlos A, Thanasoula M, Gallardo M, et al. BRCA2 acts as a RAD51 loader to facilitate telomere replication and capping. Nat Struct Mol Biol. 2010;17:1461-9 pubmed publisher
    ..protein BRCA2 is a key component of the homologous recombination pathway of DNA repair, acting as the loader of RAD51 recombinase at sites of double-strand breaks...
  38. Sun X, Brieño Enríquez M, Cornelius A, Modzelewski A, Maley T, Campbell Peterson K, et al. FancJ (Brip1) loss-of-function allele results in spermatogonial cell depletion during embryogenesis and altered processing of crossover sites during meiotic prophase I in mice. Chromosoma. 2016;125:237-52 pubmed publisher
  39. Buonomo S, Wu Y, Ferguson D, de Lange T. Mammalian Rif1 contributes to replication stress survival and homology-directed repair. J Cell Biol. 2009;187:385-98 pubmed publisher
    ..efficiency of homology-directed repair (HDR), and Rif1 deficiency results in aberrant aggregates of the HDR factor Rad51. Consistent with a role in S-phase progression, Rif1 accumulates at stalled replication forks, preferentially ..
  40. Reuter M, Zelensky A, Smal I, Meijering E, van Cappellen W, de Gruiter H, et al. BRCA2 diffuses as oligomeric clusters with RAD51 and changes mobility after DNA damage in live cells. J Cell Biol. 2014;207:599-613 pubmed publisher
    ..Diffusive behavior of fluorescent RAD51 and RAD54 was determined for comparison. All fluorescent proteins were expressed from endogenous loci...
  41. Sarkisian C, Master S, Huber L, Ha S, Chodosh L. Analysis of murine Brca2 reveals conservation of protein-protein interactions but differences in nuclear localization signals. J Biol Chem. 2001;276:37640-8 pubmed
    ..BRCA2 including its regulation during the cell cycle, localization to nuclear foci, and interaction with Brca1 and Rad51. Murine Brca2 stably interacts with human BRCA1, and the amino terminus of Brca2 is sufficient for this ..
  42. Liu K, Sun X, Feng Y, Cheng S, Li B, Li Y, et al. The impact of Zearalenone on the meiotic progression and primordial follicle assembly during early oogenesis. Toxicol Appl Pharmacol. 2017;329:9-17 pubmed publisher
    ..ZEA exposure significantly increased DNA-DSBs at the diplotene stage. Meanwhile, DNA damage repair genes such as RAD51 and BRCA1 were activated...
  43. Fukuda T, Fukuda N, Agostinho A, Hernández Hernández A, Kouznetsova A, Hoog C. STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis. EMBO J. 2014;33:1243-55 pubmed publisher
  44. Takahashi E, Matsuda Y, Hori T, Yasuda N, Tsuji S, Mori M, et al. Chromosome mapping of the human (RECA) and mouse (Reca) homologs of the yeast RAD51 and Escherichia coli recA genes to human (15q15.1) and mouse (2F1) chromosomes by direct R-banding fluorescence in situ hybridization. Genomics. 1994;19:376-8 pubmed
    We mapped the human (RECA) and mouse (Reca) homologs of the yeast RAD51 and Escherichia coli recA genes to human and mouse chromosomes by direct R-banding fluorescence in situ hybridization...
  45. Li M, Peakman M, Golub E, Reddy G, Ward D, Radding C, et al. Rad51 expression and localization in B cells carrying out class switch recombination. Proc Natl Acad Sci U S A. 1996;93:10222-7 pubmed
    b>Rad51 is a highly conserved eukaryotic homolog of the prokaryotic recombination protein RecA, which has been shown to function in both recombinational repair of DNA damage and meiotic recombination in yeast...
  46. Kakarougkas A, Ismail A, Klement K, Goodarzi A, Conrad S, Freire R, et al. Opposing roles for 53BP1 during homologous recombination. Nucleic Acids Res. 2013;41:9719-31 pubmed publisher
    ..In contrast to this role for 53BP1 in HR in G2 phase, we show that it is dispensable for HR in S phase, where HC regions are likely relaxed during replication. ..
  47. Jamsai D, O Connor A, Deboer K, Clark B, Smith S, Browne C, et al. Loss of GGN leads to pre-implantation embryonic lethality and compromised male meiotic DNA double strand break repair in the mouse. PLoS ONE. 2013;8:e56955 pubmed publisher
    ..Together, our results suggest that GGN plays a role in male meiotic DSB repair and is absolutely required for the survival of pre-implantation embryos. ..
  48. Cartwright R, Dunn A, Simpson P, Tambini C, Thacker J. Isolation of novel human and mouse genes of the recA/RAD51 recombination-repair gene family. Nucleic Acids Res. 1998;26:1653-9 pubmed
    Genes from the recA/RAD51 family play essential roles in homologous recombination in all organisms...
  49. Chen J, Silver D, Walpita D, Cantor S, Gazdar A, Tomlinson G, et al. Stable interaction between the products of the BRCA1 and BRCA2 tumor suppressor genes in mitotic and meiotic cells. Mol Cell. 1998;2:317-28 pubmed
    ..Like BRCA1 and RAD51, BRCA2 relocates to PCNA+ replication sites following exposure of S phase cells to hydroxyurea or UV irradiation...
  50. Alavattam K, Kato Y, Sin H, Maezawa S, Kowalski I, Zhang F, et al. Elucidation of the Fanconi Anemia Protein Network in Meiosis and Its Function in the Regulation of Histone Modifications. Cell Rep. 2016;17:1141-1157 pubmed publisher
    ..Our data suggest that RNF8 integrates the FA-BRCA pathway. Taken together, our study reveals distinct functions for FA proteins and illuminates the male sex chromosomes as a model to dissect the function of the FA-BRCA pathway. ..
  51. Yoshimura T, Toyoda S, Kuramochi Miyagawa S, Miyazaki T, Miyazaki S, Tashiro F, et al. Gtsf1/Cue110, a gene encoding a protein with two copies of a CHHC Zn-finger motif, is involved in spermatogenesis and retrotransposon suppression in murine testes. Dev Biol. 2009;335:216-27 pubmed publisher
    ..These observations indicate that Gtsf1/Cue110 is required for spermatogenesis and involved in retrotransposon suppression in male germ cells. ..
  52. Essers J, Hendriks R, Wesoly J, Beerens C, Smit B, Hoeijmakers J, et al. Analysis of mouse Rad54 expression and its implications for homologous recombination. DNA Repair (Amst). 2002;1:779-93 pubmed
    ..Important proteins in this pathway are Rad51 and Rad54...
  53. Hu Y, Raynard S, Sehorn M, Lu X, Bussen W, Zheng L, et al. RECQL5/Recql5 helicase regulates homologous recombination and suppresses tumor formation via disruption of Rad51 presynaptic filaments. Genes Dev. 2007;21:3073-84 pubmed
    ..To understand how RECQL5 regulates HR, we use purified proteins to demonstrate that human RECQL5 binds the Rad51 recombinase and inhibits Rad51-mediated D-loop formation...
  54. Shoji M, Tanaka T, Hosokawa M, Reuter M, Stark A, Kato Y, et al. The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline. Dev Cell. 2009;17:775-87 pubmed publisher
  55. Lee S, Roques C, Magwood A, Masson J, Baker M. Recovery of deficient homologous recombination in Brca2-depleted mouse cells by wild-type Rad51 expression. DNA Repair (Amst). 2009;8:170-81 pubmed publisher
    ..the availability, cellular localization and DNA binding activity of the central homologous recombination protein, RAD51, with loss of BRCA2 resulting in defective homologous recombination...
  56. Gudmundsdottir K, Lord C, Witt E, Tutt A, Ashworth A. DSS1 is required for RAD51 focus formation and genomic stability in mammalian cells. EMBO Rep. 2004;5:989-93 pubmed
    ..cancer susceptibility gene implicated in the repair of double-strand breaks by homologous recombination with RAD51. BRCA2 associates with a 70-amino-acid protein, DSS1, but the functional significance of this interaction has ..
  57. Uringa E, Baldeyron C, Odijk H, Wassenaar E, van Cappellen W, Maas A, et al. A mRad51-GFP antimorphic allele affects homologous recombination and DNA damage sensitivity. DNA Repair (Amst). 2015;25:27-40 pubmed publisher
    ..Disruption of the gene encoding RAD51, the protein that catalyzes DNA strand exchange during homologous recombination, results in lethality of mammalian ..
  58. Wang X, Chan K, Ming X, Lui V, Poon R, Lo C, et al. G1 checkpoint establishment in vivo during embryonic liver development. BMC Dev Biol. 2014;14:23 pubmed publisher
    ..Our study is the first to demonstrate the establishment of the DNA damage-mediated G1 cell cycle checkpoint in liver cells during embryogenesis and its in vivo biological effects during embryonic liver development. ..
  59. Urtishak K, Smith K, Chanoux R, Greenberg R, Johnson F, Brown E. Timeless Maintains Genomic Stability and Suppresses Sister Chromatid Exchange during Unperturbed DNA Replication. J Biol Chem. 2009;284:8777-85 pubmed publisher
    ..Tim deficiency led to increased H2AX phosphorylation and Rad51 and Rad52 foci formation selectively during DNA synthesis and caused a 3-4-fold increase in sister chromatid ..
  60. Ahuja A, Jodkowska K, Teloni F, Bizard A, Zellweger R, Herrador R, et al. A short G1 phase imposes constitutive replication stress and fork remodelling in mouse embryonic stem cells. Nat Commun. 2016;7:10660 pubmed publisher
    ..and Rad3 related (ATR) and is associated with chromatin loading of the ssDNA-binding proteins RPA and RAD51. Single-molecule analysis of replication intermediates reveals massive ssDNA gap accumulation, reduced fork speed ..
  61. Holt J, Pye V, Boon E, Stewart J, Garcia Higuera I, Moreno S, et al. The APC/C activator FZR1 is essential for meiotic prophase I in mice. Development. 2014;141:1354-65 pubmed publisher
  62. Souquet B, Abby E, Hervé R, Finsterbusch F, Tourpin S, Le Bouffant R, et al. MEIOB targets single-strand DNA and is necessary for meiotic recombination. PLoS Genet. 2013;9:e1003784 pubmed publisher
    ..appeared to be dispensable for the initial loading of recombinases but was required to maintain a proper number of RAD51 and DMC1 foci beyond the zygotene stage...
  63. Trojanek J, Ho T, Del Valle L, Nowicki M, Wang J, Lassak A, et al. Role of the insulin-like growth factor I/insulin receptor substrate 1 axis in Rad51 trafficking and DNA repair by homologous recombination. Mol Cell Biol. 2003;23:7510-24 pubmed
    ..This effect involves an interaction between Rad51 and the major IGF-IR signaling molecule, insulin receptor substrate 1 (IRS-1)...
  64. Dominguez Bendala J, Priddle H, Clarke A, McWhir J. Elevated expression of exogenous Rad51 leads to identical increases in gene-targeting frequency in murine embryonic stem (ES) cells with both functional and dysfunctional p53 genes. Exp Cell Res. 2003;286:298-307 pubmed
    The Rad51 gene is the mammalian homologue of the bacterial RecA gene and catalyses homologous recombination in mammalian cells. In some cell types Rad51 has been shown to interact with p53, leading to inhibition of Rad51 activity...
  65. Mizuta R, LaSalle J, Cheng H, Shinohara A, Ogawa H, Copeland N, et al. RAB22 and RAB163/mouse BRCA2: proteins that specifically interact with the RAD51 protein. Proc Natl Acad Sci U S A. 1997;94:6927-32 pubmed
    The human RAD51 protein is a homologue of the bacteria RecA and yeast RAD51 proteins that are involved in homologous recombination and DNA repair...
  66. Ganapathipillai S, Medová M, Aebersold D, Manley P, Berthou S, Streit B, et al. Coupling of mutated Met variants to DNA repair via Abl and Rad51. Cancer Res. 2008;68:5769-77 pubmed publisher
    ..growth factor Met, but not the wild-type form of the receptor, directly couple to the Abl tyrosine kinase and the Rad51 recombinase, two key signaling elements of homologous recombination-based DNA repair...
  67. Kumar R, Bourbon H, de Massy B. Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice. Genes Dev. 2010;24:1266-80 pubmed publisher
  68. Su G, Yeh H, Lin S, Chung C, Huang Y, Liu Y, et al. Role of the RAD51-SWI5-SFR1 Ensemble in homologous recombination. Nucleic Acids Res. 2016;44:6242-51 pubmed publisher
    During DNA double-strand break and replication fork repair by homologous recombination, the RAD51 recombinase catalyzes the DNA strand exchange reaction via a helical polymer assembled on single-stranded DNA, termed the presynaptic ..
  69. Suzuki A, de la Pompa J, Hakem R, Elia A, Yoshida R, Mo R, et al. Brca2 is required for embryonic cellular proliferation in the mouse. Genes Dev. 1997;11:1242-52 pubmed
    ..The similarity in phenotype between Brca1 and Brca2 mutants suggests that these genes may have cooperative roles or convergent functions during embryogenesis. ..
  70. Li M, Maizels N. Nuclear Rad51 foci induced by DNA damage are distinct from Rad51 foci associated with B cell activation and recombination. Exp Cell Res. 1997;237:93-100 pubmed
    ..previously reported that LPS-activated primary murine B cells contain nuclear foci that stain brightly with anti-Rad51 antibodies (Li et al. (1996) Proc. Natl. Acad. Sci. USA 93, 10222-10227)...